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003 ANATOMY Lectures.Qxd Abstracts www.anatomy.org.tr doi:10.2399/ana.10.019x Abstracts for the 2nd International Symposium of Clinical and Applied Anatomy, prof. Josef Stingl JUBILEE, July 9th - 11th, 2010, Prague, Czech Republic Anatomy 2010; 4 Suppl 1: 19-90 © 2010 TSACA Lectures (L-01 — L-96) Saturday, July 10 clinical disciplines, started to develop first professor Karel 08.45: Opening Lecture (Syllaba´s Hall) Weigner during the thirties of the 20th century. In his tradi- tion continued successfully several generations of Czech Moderator: Báča Václav anatomists on all seven Czech medical faculties. The most intensive advancement the Czech clinical anatomy enjoys, as L-01 both scientific as well as pedagogic discipline, during the last 30 History of the Czech clinical anatomy years, above all in connection with the intensive technological Stingl Josef development of most clinical disciplines. Department of Anatomy, Third Faculty of Medicine, Charles University in Prague, Praha, Czech Republic [email protected] 09.15: Honorary Lectures dedicated to prof. Stingl (Syllaba´s Hall) Moderator: Báča Václav The Prague University was founded 1348 as the 27th one in Europe. The Medical Faculty was its part from the very begin, L-02 but the medical curriculum had for several hundreds years a medieval scholastic form, without anatomy. The history of the Scanning electron microscopy of vascular corrosion Czech anatomy started in fact in June 1600, when professor casts: a means to study growth and regression of blood Johannes Jessenius presented the first public anatomical dissec- vessels in normal and diseased tissues and organs? tion in Prague. But the real development of the anatomy as a Lametschwandtner Alois, Bartel Heidi, Tangphokhanon full-value part of the medical curriculum began in the Wasan, Gerlach Nicolas, Minnich Bernd Bohemian Kingdom first after the reforms of the Empress Department of Organismic Biology, University of Salzburg, Maria Theresia and Emperor Josef the Second during the sec- Salzburg, Austria ond half of the 18th century. Into a real scientific discipline it [email protected] was fluently changed during the 19th century, when in Prag were acting many outstanding anatomists like Prochaska, Purkinje, Ilg, Hyrtl, Gruber, Bochdalek and others. The clini- As the transport capacity of the blood vascular system is great- cal anatomy proper, substantially oriented on the needs of the ly defined by the numbers, the dimensions, the hierarchical Copyright © 2010 Turkish Society of Anatomy and Clinical Anatomy (TSACA). All rights reserved. Published by Deomed Medical Publishing, Istanbul. 20 2nd International Symposium of Clinical and Applied Anatomy, July 9th - 11th, 2010, Prague, Czech Republic order, and the three-dimensional arrangement of its compo- dents includes demonstrations of arthroscopies, laparoscopies, nents, i.e. arteries, arterioles, capillaries, venules, and veins, bronchoscopies and gastroscopies. For young physicians the quantification of vessel diameters, vessel lengths, interbranch- centre provides an advanced approach to the practical training ing distances, and branching angles is of utmost importance. In in the field of mini-invasive and endoscopic methods. The 1971 the scanning electron microscope was introduced to the Centre represents the maximum progress achievable in the study of vascular corrosion casts made from a polymerizing clinical anatomy teaching for students and physicians. E-learn- resin and thus vascular beds could be described qualitatively ing creates a possibility of distant theoretical education before from the macroscopical to the microscopical level with high practical experience with cadaver “hands-on” courses. spatial resolution. Since then new resins with refined physico- Grant support: MŠMT 14/71/6b/B/2010 chemical properties and improved casting protocols, together with the availability of computer-based 3D-morphometry sys- tems, enabled comprehensive spatial measurements of the above mentioned variables from real existing vascular networks 09.45: Session 1 - Invited Lectures (Jonáš´s Hall) from stereopaired scanning electron micrographs. Finally, by Moderator: Tonar Zbyněk, Kluchová Darina, Krivokuća Dragan careful observations and time course studies of growing and regression vascular beds of normal tissues and organs by SEM L-04 of vascular corrosion casts in combination with correlative light Quantification of microvessels in normal and tumorous microscopy sprouting and non-sprouting angiogenesis (= intus- organs susceptive microvascular growth; IMG) as well as vascular 1 2 3 regression could be resolved and demonstrated with unsur- Tonar Zbyněk , Witter Kirsti , Kochová Petra , 4 passed clarity. The lecture will give examples and discuss Boudová Ludmila potentials and limitations of SEM of vascular corrosion casting. 1Department of Histology and Embryology, Faculty of Medicine in 2 A grant from the Fonds zur Förderung der Wissenschaftlichen Pilsen, Charles University in Prague, Pilsen, Czech Republic; Institute of Forschung, Project 19050 is greatly acknowledged. Histology and Embryology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria; 3Department of Mechanics, Faculty of Applied Sciences, University of West Bohemia in Pilsen, Pilsen, Czech Republic; 4Department of Pathology, Faculty of Medicine L-03 in Pilsen, Charles University in Prague, Pilsen, Czech Republic Educational centre for mini-invasive techniques at the [email protected] Department of Anatomy, Third Faculty of Medicine, Charles University in Prague: the example of clinical In both normal and pathological histology, microvascular bed is anatomy teaching in practice often investigated in order to assess the quality of perfusion of Báča Václav, Doubková Alena, Kachlík David various organs, to devise models of vascular network, to detect Department of Anatomy, Third Faculty of Medicine, Charles age-related changes, to assess angiogenesis and its relation to University in Prague, Praha, Czech Republic inflammation and growth of tumours. Our aim is to review stereological methods available for unbiased quantification of [email protected] microscopic blood vessels in histological sections. The first step is to perform sufficient multistage systematic uniform random During last ten years a unique multimedia educational centre sampling of tissue blocks, histological sections and microscopic for topographic and clinical anatomy, endoscopy and micro- fields of view. The second step is to visualize the microvessels surgery was developed at our Department of Anatomy. A new with histochemistry against basal or reticular lamina or, prefer- method of impregnation allowing a broader use of cadavers in ably, with endothelial markers, which are often organ- and interventional medicine has been initiated in 2002. This species-specific. The third step is to find a quantitative parame- method was developed by Walter Thiel from Graz. One room ter useful for relevant biological question, such as length densi- of our dissection tract has been converted into an operating ty, tortuosity, surface density or volume fraction of the theatre with full equipment for mini-invasive techniques – rigid microvessels. In series of sections, the orientation and and flexible endoscopic towers with instruments, operative anisotropy can be statistically assessed. When quantifying the table, operative microscope, X-ray C-arm, etc. The premises of valence and numerical density of branching nodes, capillary the dissection wing were interconnected by an audio-video net- density can be computed. Microvessels are often routinely work with a wireless connection to the Internet between 2003 quantified in individual histological sections as number of pro- and 2006. There is also a graphic studio for creation of materi- files per section area (“microvessel density”). All the steps are als for instruction. E-learning module project for the Centre illustrated with examples of analysis of microvessels in human started in 2008. Recently, dissection training of medical stu- brain, in tumorous lymph nodes and quantification of vasa vaso- Anatomy 2010; 4 Suppl 1 Abstracts 21 rum in large blood vessels. Describing morphological properties Exhibition of plastinated human bodies and body parts, which of microvessels with use of continuous variables permits us to has been touring around the world with constantly updated apply standard statistical procedures and tests to morphometric collection, shocks and fascinates people worldwide. There is a data. hard legal and ethical debate on somebody’s right to display The study was supported by the Project MSM4977751303, the objects that used to be living persons, and who are (according GACR Project No. 106/09/0740 and the Project KONTAKT to the author himself) »stopped somewhere between death and ME09090. final decomposition« in such manner. The conservatives claim about blasphemy, mockery of the divine and the human, whereas the more liberal ones consider his work a »facing with the reality in a radical way and overcoming the death-corps L-05 taboo«. Thousands of people decided to allow plastination of Effects of prenatal application of retinoic acid their bodies in a manner presented at this exhibition. The idea of everybody’s right to dedicate ones body to whatever purpose Kluchová Darina he wants poses the question, whether plastination, primarily Department of Anatomy, Faculty of Medicine, P.J. Safarik University, focused on scientific benefit, will become a mass production
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