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Report on the Workshop in Diagnosis of the Exotic Fish Diseases EHN and EUS and the 12Th Annual Meeting of the National Reference Laboratories for Fish Diseases

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Report on the Workshop in Diagnosis of the Exotic Fish Diseases EHN and EUS and the 12Th Annual Meeting of the National Reference Laboratories for Fish Diseases Report on the Workshop in diagnosis of the exotic fish diseases EHN and EUS and the 12th Annual Meeting of the National Reference Laboratories for Fish Diseases Aarhus, Denmark June 17-20, 2008 Organised by the Community Reference Laboratory for Fish Diseases National Veterinary Institute, Technical University of Denmark Report from the 12th Annual Meeting of the National Reference Laboratories for fish diseases, 17-20 June 2008, Aarhus, Denmark Contents Introduction and short summary ............................................................................................................4 Programme .............................................................................................................................................5 Programme .............................................................................................................................................6 SESSION IA: Epizootic haematopoietic necrosis virus (EHNV)..........................................................9 Epizootic haematopoietic necrosis (EHN) disease – a review...........................................................9 Comparison of isolation procedures for ranavirus in fish organs. ...................................................10 How to detect EHNV and related ranaviruses using IHC and IFAT ..............................................11 Ranaviruses - Molecular detection and differentiation ....................................................................12 SESSION IB: Epizootic Ulcerative Syndrome (EUS).........................................................................13 Introduction to Epizootic Ulcerative Syndrome - EUS....................................................................13 EUS Outbreak Situations and Recent Outbreaks in Africa..............................................................15 Morphological differences between Aphanomyces invadans and closely related Oomycetes ........16 Susceptibility of three European freshwater fish species to Aphanomyces invadans......................17 Comparative pathology of fungal and fungal-like infections of fish ...............................................18 Field and Laboratory Investigation of EUS Outbreak .....................................................................19 Guidelines - Isolation and identification of Aphanomyces invadans from EUS affected fish......20 SESSION II: Procedures for diagnosing EHN and EUS – a practical approach.................................22 Platform 1: EUS histological slides examination and Video...........................................................22 Platform 5: Immunological methods used for diagnosis of EHNV. ................................................24 Platform 5: Immunological methods used for diagnosis of EHNV. ................................................25 SESSION III: Update on important fish diseases in Europe and their control ....................................27 Survey & Diagnosis of listed fish diseases in the European Community 2007...............................27 Outbreak of viral haemorrhagic septicaemia (VHS) in sea farmed rainbow trout in Norway ........29 VHS outbreak in Slovenia................................................................................................................30 Eradication of VHS in Denmark......................................................................................................31 Surveillance and eradication of fish diseases...................................................................................32 Prevalence of low pathogenic Infectious Salmon Anaemia Virus of the HPR0 subtype in Farmed Atlantic Salmon (Salmo Salar L) in the Faroes ...............................................................................33 The Health situation in Farmed Fish in Norway 2007.....................................................................34 PD update from Norway ..................................................................................................................36 Fish diseases situation in Spain........................................................................................................37 The isolation of Spring Viraemia of carp virus, in Romania, in a clinical outbreak of disease in carp...................................................................................................................................................38 First outbreak of IHN in the Netherlands.........................................................................................39 SESSION IV: Technical issues related to sampling and diagnosis .....................................................39 Risk Based surveillance ...................................................................................................................39 Establishment of a new Commission Decision on sampling and diagnostic procedures of listed diseases.............................................................................................................................................40 Sampling procedures for molecular based methods, as PCR, in practical fish diagnostics.............41 Development and application of real-time PCR assays for the detection of viral haemorrhagic septicaemia virus (VHSV). ..............................................................................................................42 Serological methods recommended for detection of antibodies against VHSV and IHNV ............43 2 Report from the 12th Annual Meeting of the National Reference Laboratories for fish diseases, 17-20 June 2008, Aarhus, Denmark Experiences of a micro-dilution method for testing antimicrobial susceptibility of bacteria from fish....................................................................................................................................................43 OIE recommended procedures for KHV diagnosis and the KHV PCR ring-trial 2007 ..................47 Infectious salmon aneamia as a non-exotic disease in the EU: Consequences for active surveillance ..........................................................................................................................................................48 Health categorisation and implementation of risk based surveillance in Denmark.........................49 Health categorisation and implementation of risk based surveillance in Italy.................................49 SESSION V: Scientific research update ..............................................................................................50 Establishment of database for fish pathogenic viruses ....................................................................50 ICES – Working Group on Pathology and Diseases of Marine Organisms (WGPDMO)...............52 Recent advances in fish immunology and virology .........................................................................54 KHV diagnostics and vaccination against KHVD...........................................................................55 SESSION VI: Update from the CRL ...................................................................................................56 Presentation of new CRL-fish web page..........................................................................................56 The CRL Inter-laboratory Proficiency tests 2007............................................................................57 A comparative sequence study launched in association with the proficiency test 2007..................58 Report from Year 2007 and work plan for 2008 and 2009..............................................................60 Next meeting and end of 12th Annual Meeting ................................................................................61 3 Report from the 12th Annual Meeting of the National Reference Laboratories for fish diseases, 17-20 June 2008, Aarhus, Denmark Introduction and short summary In June 17th-20th 2008, the 12th annual meeting of the National Reference Laboratories for fish diseases was held back-to-back with a workshop in diagnosis of the exotic fish diseases Epizootic hematopoietic necrosis (EHN) and Epizootic ulcerative necrosis (EUS) at the National Veterinary Institute in Aarhus, Denmark. A total number of 64 participants from 32 countries attended over the four day period. There were a total of five theoretical sessions and one practical session. In total, there were 40 presentations, 13 of which were given by invited speakers and five working platforms, of which four were run by invited instructors. All theoretical sessions were held at Aarhus University close by our laboratory as the number of participants have grown to a size that we do no more have facilities for housing all in-house. The practical session and meals where, however, organised in the laboratory making a very crowded and lively day for all! At the workshop, the first session focused on providing the participants with a theoretical introduction to the two diseases EHN and EUS that have been listed as exotic diseases in the new Council Directive 2006/88/EC. The introduction to EHNV was provided by four experts from three different laboratories in Europe. The EUS part was organised with the help from two experts from the OIE reference laboratory, in Bangkok, Thailand for EUS and two European experts. At session II, the practical part of the workshop, five working platforms were designed. Two platforms concerned EHN diagnosis, two concerned EUS diagnosis and one were common for the two diseases. Participants were divided
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