Molecular Analysis of the Genetic Diversity of Chinese Hami Melon and Its Relationship to the Melon Germplasm from Central and South Asia
J. Japan. Soc. Hort. Sci. 80 (1): 52–65. 2011. Available online at www.jstage.jst.go.jp/browse/jjshs1 JSHS © 2011 Molecular Analysis of the Genetic Diversity of Chinese Hami Melon and Its Relationship to the Melon Germplasm from Central and South Asia Yasheng Aierken1,2, Yukari Akashi1, Phan Thi Phuong Nhi1, Yikeremu Halidan1, Katsunori Tanaka3, Bo Long4, Hidetaka Nishida1, Chunlin Long4, Min Zhu Wu2 and Kenji Kato1* 1Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan 2Hami Melon Research Center, Xinjiang Academy of Agricultural Science, Urumuqi 830000, China 3Research Institute for Humanity and Nature, Kyoto 603-8047, Japan 4Kunming Institute of Botany, CAS, Heilongtan, Kunming, Yunnan 650204, China Chinese Hami melon consists of the varieties cassaba, chandalak, ameri, and zard. To show their genetic diversity, 120 melon accessions, including 24 accessions of Hami melon, were analyzed using molecular markers of nuclear and cytoplasmic genomes. All Hami melon accessions were classified as the large-seed type with seed length longer than 9 mm, like US and Spanish Inodorus melon. Conomon accessions grown in east China were all the small- seed type. Both large- and small-seed types were in landraces from Iran, Afghanistan, Pakistan, and Central Asia. Analysis of an SNP in the PS-ID region (Rpl16-Rpl14) and size polymorphism of ccSSR7 showed that the melon accessions consisted of three chloroplast genome types, that is, maternal lineages. Hami melon accessions were T/338 bp type, which differed from Spanish melon and US Honey Dew (T/333 bp type), indicating a different maternal lineage within group Inodorus.
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