DOCSLIB.ORG

Communication 0100

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Communication 0100 COMMUNICATIONUS009731012B2 0100 (12 ) United States Patent (10 ) Patent No. : US 9 , 731, 012 B2 Arany et al. ( 45) Date of Patent: Aug . 15 , 2017 ( 54 ) LASER -ACTUATED THERAPEUTIC .. .. .. .. .. .. .. .. .. .. .. .. 424 / 400 ; 514 / 1 . 1 , 8 . 9 NANOPARTICLES See application file for complete search history . (71 ) Applicant : President and Fellows of Harvard (56 ) References Cited College, Cambridge , MA (US ) U . S . PATENT DOCUMENTS (72 ) Inventors: Praveen Arany , Cambridge , MA (US ) ; 2010 /0183728 Al * 7 /2010 Desai .. .. .. A61K 9 /0019 David J . Mooney , Sudbury , MA (US ) 424 /489 2010/ 0196490 A1 * 8 / 2010 Desai .. A61K 9 /0019 (73 ) Assignee : President and Fellows of Harvard 424 / 489 College , Cambridge , MA (US ) 2010 /0226996 A1* 9 / 2010 Desai A61K 9 /0019 424 /499 2012 /0076862 A1* 3 /2012 Desai . .. .. .. .. A61K 31 / 337 ( * ) Notice : Subject to any disclaimer , the term of this 424 / 491 patent is extended or adjusted under 35 2013 /0231287 A1 * 9 /2013 Nacharaju . .. .. A61K 47 /48215 U . S . C . 154 ( b ) by 98 days. 514 / 15 . 2 (21 ) Appl. No .: 14 / 389 , 136 OTHER PUBLICATIONS (22 ) PCT Filed : Mar . 12 , 2013 Arany et al. , Wound Repair Regen , 15 ( 6 ) :866 - 74 . (2007 ) “ Activa tion of latent TGF- betal by low -power laser in vitro correlates with ( 86 ) PCT No. : PCT/ US2013 / 030493 increased TGF -betal levels in laser -enhanced oral wound healing .” Arany , P . R ., Proceedings of Light- Activated Tissue Regeneration $ 371 ( c ) ( 1 ) , and Therapy Conference Lecture Notes in Electrical Engineering ( 2 ) Date : Sep . 29 , 2014 vol . 12 , pp . 207 - 212 . ( 2008 ) “ Photobiomodulation by Low Power Laser Irradiation Involves Activation of Latent TGF -B1 ” . (87 ) PCT Pub. No. : W02013 / 148158 Jeong et al ., Theranostics , 1 : 230 -239 , ( 2011) . “ Photosensitizer Conjugated human serum albumin nanoparticles for effective PCT Pub . Date : Oct . 3 , 2013 photodynamic therapy . ” Kratz et al. , J Control Release 132 ( 3 ) : 171 -83 . ( 2008 ) . “ Albumin as (65 ) Prior Publication Data a drug carrier : design of prodrugs , drug conjugates and nanoparticles .” US 2015 /0065685 A1 Mar. 5 , 2015 Lu et al. , J Control Release , 107 ( 3 ) :428 -48 . ( 2005 ) " Cationic albumin - conjugated pegylated nanoparticles as novel drug carrier Related U . S . Application Data for brain delivery. ” ( 60 ) Provisional application No . 61/ 617 ,822 , filed on Mar . 30 , 2012 . * cited by examiner (51 ) Int. CI. Primary Examiner — David Lukton A61K 47 / 48 ( 2006 .01 ) (74 ) Attorney, Agent, or Firm - McCarter & English , A6IK 41/ 00 ( 2006 . 01 ) LLP ; Maria Laccotripe Zacharakis ; Deborah L . Nagle A61K 38 / 18 ( 2006 .01 ) A61K 38 /00 ( 2006 .01 ) (57 ) ABSTRACT (52 ) U . S . CI. The invention provides compositions and methods for laser CPC . .. .. A61K 41/ 0042 ( 2013 .01 ) ; A61K 38 / 1841 actuated drug delivery . Compositions comprise serum albu (2013 . 01 ) ; A61K 47 /48284 (2013 .01 ) ; A61K min based particles conjugated with therapeutic agents 47/ 48884 ( 2013 .01 ) ; A61K 38 / 00 (2013 .01 ) which cab become bioavailable upon actuation of the par (58 ) Field of Classification Search ticles by light , e . g . low power laser . CPC .. A61K 47 /48884 ; A61K 41/ 0042 ; A61K 38 / 00 ; A61K 38 / 1841 ; A61K 47/ 48284 17 Claims, 24 Drawing Sheets atent Aug . 15 , 2017 Sheet 1 of 24 US 9 ,731 , 012 B2 mgWater • BSA CONTROL ABSA LASER hobi A mDeg N2 teelDDIDDIWDLIDATI XXX << < < < < < << < << < < < < < < << < < < < < < < << < < ** * * * * 3 modae con 190 200 210 220 230 240 250 260 WAVELENGTH (nm ) A FIG . 1 LASER 9. 9903 INFLAMMATION 80FIG . 2 U . S . Patent Aug . 15, 2017 Sheet 2 of 24 US 9 ,731 , 012 B2 HSA DRUGHSACONJUGATE HSA ANTIBODY-BINDING COMPLEXES >=PEPTIDE DRUG=O ODXC drugsasconjugates,complexesornanoparticles.FiguetakenfromKratzetal2011[1] Applicationsofalbuminasadrugcarrierfordeveloped-peptide,antibodyorbased FIG.3 HSA=HUMAN SERUMALBUMIN HSA Albuminasadrugcarrier HSAODvd DRUGHSACOMPLEX DRUGHSANANOPARTICLES o=PEPTIDE O=DRUG U . S . Patent Aug . 15, 2017 Sheet 3 of 24 US 9 ,731 , 012 B2 EFFECTORFUNCTIONS EFFECTORFUNCTION CHEMOTAXIS #SURVIVAL OPROLIFERATION ODIFFERENTIATION GENERATIONINTHEPERIPHERY EFFECTORFUNCTIONS I FoxP3EXPRESSION CHEMOTAXIS TCELL OHOMEOSTASIS 70°°°\ PMN Treg FOR EFFECTORFUNCTIONS EO NKCELL HOMEOSTASISOCYTOKINEPRODUCTION CYTOTOXICITY FIG.4 CHEMOTAXIS 008 BCELL MO/PRESENTATION)ANTIGENO DO SWITCHINGIgA EFFECTORFUNCTIONS zn OPROLIFERATION OACTIVATION OSURVIVAL +CHEMOTAXISOSCAVENGERFUNCTION LANGERHANSCELLDEVELOPMENT ANTIGENPRESENTATION FUNCTIONSOFTGF-B OMATURATION OANTIGENPRESENTATION STIMULATES?MAINTAINS OINHIBITS UNCLEAR U . S . Patent Aug . 15, 2017 Sheet 4 of 24 US 9 , 731, 012 B2 - 1 . , ' . : / FIG . 5 INITIAL CONDITIONIN BSABSA NPNP LOADING FRACTION 1ST ( CONJUGATION SOLUTION ) CENTRIFUGE TGF- B1 - ( LUTE - - ( UNCONJUGATED FRACTION ) ACTIVATION CONTROL LASER BOUND FRACTION ACTIVATION (CONTROL )( LASER H202/ 0TT (BOUND BIOAVAILABILITY ) FIG . 6 U . S . Patent Aug . 15 , 2017 Sheet 5 of 24 US 9 . 731 , 012 B2 4 1 ? LaLau 1 . : FIG.7 . .”: . ? , " : s : : * : : : HLP ' rik . ru U . S . Patent Aug . 15, 2017 Sheet 6 of 24 US 9 ,731 , 012 B2 ?? ? 0PTICALDENSITY(570nm) ? ? ? ? mg 7? _ 30 _ 80 _ 80 120 150 TIME ( IN) FIG. 8 - * - ' . ' * . " . ' . ' , ' , ' , ' , ' . ' . FIG. 9 atent Aug . 15 , 2017 Sheet 7 of 24 US 9 , 731 , 012 B2 ??? FIG.11D FIG.11c ?????? FIG.10 FIG.11B ??FIG.11A U . S . Patent Aug . 15, 2017 -Sheet 8 of -24 US 9 ,731 , 012 B2 INTENSITY(%) ttt -- w 7. mm LLLLLLLLLLL kuLL Ik LILI OL 100 0001 00001 SIZE (d . nm ) FIG . 12A INTENSITY(%)2 WILL - 10 001 0001 00001 SIZE ( d. nm )Oh . .. oo .. FIG . 12B INTENSITY(%) 5.749- .. .. .. .. .. .vara LLLL . .. .. .. .. .. .. .. L 100 0001 00001 SIZE (d .nm ) FIG . 120 atent Aug . 15 , 2017 Sheet 9 of 24 US 9 ,731 , 012 B2 100 um FIG . 13A PROFILE EXTRACTION 1 www - * - ultimatethatthetitleoftwoother - ! ALTITUDE(nm) * than * - - - - - the ASUSUNANISASIUMISMASNENUSTASSNIUS - -77777777777777777777F7777777 - siwww - wwwwwwwwwwwww il why DISTANCE: 789. 68 um . MINIMUM ALTITUDE: - 3. 3 nm - - - - - - - - - - - - - - - - - - - MAXIMUM ALTITUDE: 31. 0 nm AVERAGE ALTITUDE : 2 . 9 nm MTWITTTTTTTTTTTTTTTTTT0 100 200 300 400 500 600 700 DISTANCE (um ) FIG . 13B FIG . 14 U . S . Patent Aug . 15, 2017 Sheet 10 of 24 US 9 ,731 ,012 B2 CARBON 80% SULFUR FIG.15B 8% SODIUM y CHLORINE 11% .111 IM 52% " 7. CHLORINE W SULFUR 0% FIG.15A SODIUM 27% ** 21% * CARBON U . S . Patent Aug . 15, 2017 Sheet 11 of 24 US 9 ,731 , 012 B2 MAANAAAAAAAAHHH FIG . 16A FIG . 16B FIG . 16C * UM)CYSTEINE(FREE =???? CONTROL H202 BSA FIG . 17 U . S . Patent Aug . 15 , 2017 Sheet 12 of 24 US 9 ,731 , 012 B2 * FREECYSTEINES(UM) q????? CONTROL H202 CONTROL BSABC NPS H202 FIG . 18 FOLDINCREASEOVERCONTROL(RFU) 105 CONTROL LASER BSABSA MO NPS LASER FIG . 19A * FOLDINCREASEOVERCONTROL(UM) po-op CONTROL LASER BSA NPS FIG . 19B U . S . Patent Aug . 15, 2017 Sheet 13 of 24 US 9 ,731 , 012 B2 INTENSITY(%) 27.42 TT .. .. TITIITTIT TTTTTTTTTTI 10001000 1000010000 FIG SIZE. ( d.20A nm ) INTENSITY(%) TTC TTTTTTT TTTTTTT TTTTTTT TTTTT T TTTTTTT 10 100100 1000100001000 10000 SIZE (d . nm ) FIG . 20B INTENSITY(%) 1 27.9 TIT TTTTT .. quam TIIM 10 100 1000 10000 SIZE ( d .nm ) FIG . 200 INTENSITY(%) 27.9?š TTTTTTTTTTTTTTTT TTTTTTT . 100 1000 10000 SIZE ( d .nm ) FIG . 20D U . S . Patent Aug . 15, 2017 Sheet 14 of 24 US 9 ,731 , 012 B2 FOLDINCREASE(DECREASE) OVERCONTROL(RFUS) * -02 CONTROL CONJUGATED BSA - EMBEDDED BSA TGF- b1 NP TGF- b1 NP FIG . 21 FOLDINCREASEOVERCONTROL(RFUS) CONTROL LASER CONJUGATED BSA - TGF- b1 NPS FIG . 22 U . S . Patent Aug . 15, 2017 Sheet 15 of 24 US 9 ,731 , 012 B2 BSA NPS BSA -TGF -B1 NPS CONTROL LASER CONTROL LASER FIG . 23 TGFb-1(pg/mL) § CONTROL LASER CONJUGATED BSA - TGFb - 1 NANOPARTICLES FIG . 24 na manatent Augwo . 15 , 2017 menSheet 16 of 24 US 9 ,731 , 012 B2 FOLDINCREASEOVERCONTROLS (LUCIFERASEACTIVITY) anon CONTROL 3J/ cm2 30J / cm2 LASER CONJUGATED BSA - TGFb- 1 NANOPARTICLES buFIG . 25 12000 10000 8000 LUCIFERASEACTIVITY 6000 4000 D 2000 Do ZŠ Š 10 TGF- B1 CONCENTRATION (ng /mL ) FIG . 26 U . S . Patent Aug. 15 , 2017 Sheet 17 of 24 US 9 ,731 , 012 B2 SEAP(WU/RFU*106 Incopara CONTROL LPSLPS LPS + TGF -b1 FIG . 27 SEAP(WU/RFU*106) qon# CONTROL LPS LASER + LPS CONJUGATED BSA - TGF- b1 NPS FIG . 28 centeU . S . Patent -Aug . 15, 2017 -Sheet 18 of 24 US 9 ,731 ,012 B2 LPS +TGF-b1 LASER+LPS CONTROLLPS3J/cm230J CONJUGATEDBSA-TGFb1NPS LPS FIG.29B FIG.30B ND CONTROLCONTROL ND 450 40043507 300 250 200 1507-4100 50 0+ 900 800 700 600500 400 300 200 100- )104 * RFU- mL / pg ( 1b- IL ) 104 * RFU- mL / pg ( 1b -IL LPS +TGF-b1 LASER+LPS CONTROLLPS3J/cm230J CONJUGATEDBSA-TGFb1NPS LPSLPS FIG.29A FIG.30A CONTROL 800, 700-4 600 500 300 200 100 3000 2500 2000 1500 000 66)104 * RFU- mL / pg ( a- TNF ) 104 * RFU -mL / pg ( a -TNF U . S . Patent Aug . 15, 2017 Sheet 19 of 24 US 9 ,731 , 012 B2 100mg/ml 10mg/ml 2000FIG.31 atent Aug . 15 , 2017 Sheet 20 of 24 US 9 ,731 , 012 B2 NPPBS HOURS24 HOURS5 MIN90 MIN60 MIN45 MIN30 MIN25 MIN20 ??????? MIN15 MIN405 WNIWO BASELINE FIG.32A 2.50E+08 2.00E+08 0850E+.1 1.00E+08 5.00E+07 0.00E+00 )sr /cm2 /s /p ( RADIANCE AVG CONTROL:LATEXBEADS CARRAGEENAN 09710e+1.ROI2-09621e+ROI-5.1HOURS4 U . S . Patent Aug . 15, 2017 Sheet 21 of 24 US 9 ,731 ,012 B2 NP aPBS 7DAY 5DAY 3DAY 1DAY 0.6DAY FIG.32B ) sr/ cm2 /s / p( RADIANCE AVG CARRAGEENAN CONTROL:SELECTIN-BASED 4HOURS U . S . Patent Aug . 15, 2017 Sheet 22 of 24 US 9 ,731 ,012 B2 PBS-NP 7DAY 5DAY 3DAY 1DAY 0.6DAY 07 FIG.32C )sr / cm2 / s / p ( RADIANCE AVG NOTEXIN CONTROL:MPO-BASED 3DAYS atent Aug . 15 , 2017 Sheet 23 of 24 US 9 ,731 , 012 B2 DAYO DAY2 + 4HRS + 15MIN DAY3 SEED IMPLANT & INJECT LASER LUCIFERASE HIIIISCAFFOLD CARREGENNAN B1: BSANP IMAGING FIG . 33A FIG . 33B e TGFR1 BSA NP FIG . 33C Us Patentatent soAug . 15 , 2017we Sheetmus 24 of 24 US 9 ,731 , 012 B2 DAYO DAY5 DAY10 DAY12 IMPLANT INJECT B1- BSA NP OR PBS INJECT B1- BSA NP OR PBS SACRIFICE & PEI SCAFFOLD & LASER TREATMENT & LASER TREATMENT ANALYZE - FIG .
Load more

Recommended publications
  • NNT '09 Program
    NNT '09 Program 9-Nov Wednesday, November 11 12:00 Exhibit set-up, Room J2/J3, San Jose Convention Center 12:00 Registration 17:00 Welcome Reception and Equipment Exhibit, Room J2/J3, San Jose Convention Center Thursday, November 12, Room J1/J4 , San Jose Convention Center Plenary Session - Session Chairs: S. Chou (Princeton) & L. Montelius (Lund) 8:15 Welcome: Stephen Chou and Christie Marrian 8:30 Plenary NNT is Losing the Propaganda War Fabian Pease Stanford University 9:00 Invited 1 Template Infrastructure for Nanoimprint Lithography Nobuhito Toyama Dai Nippon Printing 9:20 Invited 2 NaPANIL: Consolidation of Nanoimprinting for Production Jouni Ahopelto VTT Microsystems and Nanoelectronics 9:40 Invited 3 Shrink-Induced Nanostructures Michelle Khine University of California, Irvine 10:00 Break Magnetics/Biology/Solar - Session Chairs: G. Willson (U. Texas) & H. Schift (PSI) 10:30 Invited 4 The Nano-imprinting Process towards Patterned Media Manufacturing Tsai-Wei Wu HGST 10:50 c1 Large Scale Fabrication of Nanoimprinted Magnetic Nanoparticles with Self-Assembled Templates Wei Hu Stanford University 11:05 c2 Photocatalytic Nanolithography: An Emergent Patterning Technique Relevant to Biotechnology Jane P Bearinger LLNL 11:20 c3 Fabrication of 3D Cell Containers with Integrated Topography by Combined Microscale Arne Schleunitz Paul Scherrer Institut Thermoforming and Thermal Nanoimprint 11:35 c4 Nanoimprinting of Subphthalocyanines for Photovoltaic Applications Xiaogan Liang Lawrence Berkeley National Laboratory 11:50 c5 Nanopatterned anode for organic solar cell by nanoimprint Dae-Geun Choi Korea Institute of Machinery & Materials 12:05 Lunch Electronics/Optoelectronics - Session Chairs: J. Randall (Zyvex) and S. Matsui (Hyogo) 13:30 Invited 5 Nanoimprint lithography for organic thin film transistors Barbara Stadlober Joanneum Research 13:50 c6 Fabrication of organic TFT arrays on an A4-sized flexible sheet using microcontact printing Hiroshi Fujita Dai Nippon Printing Co., Ltd.
    [Show full text]
  • Etude Des Techniques De Super-Résolution Latérale En Nanoscopie Et Développement D’Un Système Interférométrique Nano-3D Audrey Leong-Hoï
    Etude des techniques de super-résolution latérale en nanoscopie et développement d’un système interférométrique nano-3D Audrey Leong-Hoï To cite this version: Audrey Leong-Hoï. Etude des techniques de super-résolution latérale en nanoscopie et développement d’un système interférométrique nano-3D. Micro et nanotechnologies/Microélectronique. Université de Strasbourg, 2016. Français. NNT : 2016STRAD048. tel-02003485 HAL Id: tel-02003485 https://tel.archives-ouvertes.fr/tel-02003485 Submitted on 1 Feb 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. UNIVERSITÉ DE STRASBOURG ÉCOLE DOCTORALE MATHEMATIQUES, SCIENCES DE L'INFORMATION ET DE L'INGENIEUR (MSII) – ED 269 LABORATOIRE DES SCIENCES DE L'INGENIEUR, DE L'INFORMATIQUE ET DE L'IMAGERIE (ICUBE UMR 7357) THÈSE présentée par : Audrey LEONG-HOI soutenue le : 2 DÉCEMBRE 2016 pour obtenir le grade de : Docteur de l’université de Strasbourg Discipline / Spécialité : Electronique, microélectronique, photonique Étude des techniques de super-résolution latérale en nanoscopie et développement d'un système interférométrique nano-3D THÈSE dirigée par : Dr. MONTGOMERY Paul Directeur de recherche, CNRS, ICube (Strasbourg) Pr. SERIO Bruno Professeur des Universités, Université Paris Ouest, LEME (Paris) RAPPORTEURS : Dr. GORECKI Christophe Directeur de recherche, CNRS, FEMTO-ST (Besançon) Pr.
    [Show full text]
  • Multiplexed Biomimetic Lipid Membranes on Graphene by Dip-Pen Nanolithography
    ARTICLE Received 17 May 2013 | Accepted 10 Sep 2013 | Published 10 Oct 2013 DOI: 10.1038/ncomms3591 OPEN Multiplexed biomimetic lipid membranes on graphene by dip-pen nanolithography Michael Hirtz1,*, Antonios Oikonomou2,*, Thanasis Georgiou3, Harald Fuchs1,4 & Aravind Vijayaraghavan5 The application of graphene in sensor devices depends on the ability to appropriately func- tionalize the pristine graphene. Here we show the direct writing of tailored phospholipid membranes on graphene using dip-pen nanolithography. Phospholipids exhibit higher mobility on graphene compared with the commonly used silicon dioxide substrate, leading to well-spread uniform membranes. Dip-pen nanolithography allows for multiplexed assembly of phospholipid membranes of different functionalities in close proximity to each other. The membranes are stable in aqueous environments and we observe electronic doping of graphene by charged phospholipids. On the basis of these results, we propose phospholipid membranes as a route for non-covalent immobilization of various functional groups on graphene for applications in biosensing and biocatalysis. As a proof of principle, we demonstrate the specific binding of streptavidin to biotin-functionalized membranes. The combination of atomic force microscopy and binding experiments yields a consistent model for the layer organization within phospholipid stacks on graphene. 1 Institute of Nanotechnology (INT) and Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany. 2 School of Computer Science and Centre for Mesoscience and Nanotechnology, The University of Manchester, Manchester M13 9PL, UK. 3 School of Physics and Astronomy, The University of Manchester, Manchester M13 9PL, UK. 4 Physical Institute and Center for Nanotechnology (CeNTech), University of Mu¨nster, Wilhelm-Klemm-Str.
    [Show full text]
  • (12) United States Patent (10) Patent No.: US 8,088,615 B2 Ausserre (45) Date of Patent: Jan
    USOO8088615B2 (12) United States Patent (10) Patent No.: US 8,088,615 B2 Ausserre (45) Date of Patent: Jan. 3, 2012 (54) OPTICAL COMPONENT FOR OBSERVINGA (56) References Cited NANOMETRIC SAMPLE, SYSTEM COMPRISING SAME, ANALYSIS METHOD U.S. PATENT DOCUMENTS USING SAME, AND USES THEREOF 5,639,671 A * 6/1997 Bogartet al. ................. 436,518 (76) Inventor: Dominique Ausserre, Soulitre (FR) 5,962,114 A * 10/1999 Jonza et al. ........ ... 428.212 6,034,775 A * 3/2000 McFarland et al. ............. 506/12 (*) Notice: Subject to any disclaimer, the term of this 6,312,691 B1 * 1 1/2001 Browning et al. ......... 424,143.1 patent is extended or adjusted under 35 6,493.423 B1* 12/2002 Bisschops .............. ... 378,119 U.S.C. 154(b) by 777 days. 6,697,194 B2 * 2/2004 Kuschnereitet al. ... 359,359 6,710,875 B1* 3/2004 Zavislan ............ ... 356,364 (21) Appl. No.: 11/631,846 6,718,053 B1 * 4/2004 Ellis et al. ... ... 38.2/128 Jul. 6, 2005 6,853,455 B1* 2/2005 Dixon et al. ... 356/453 (22) PCT Filed: 6,934,068 B2 * 8/2005 Kochergin ... ... 359,280 (Under 37 CFR 1.47) 7.209,232 B2 * 4/2007 Ausserre et al. 356,369 7,258,838 B2* 8/2007 Lietal. .......... ... 422.68.1 (86). PCT No.: PCT/FR2OOS/OO1746 7,265,845 B2 * 9/2007 Kochergin . ... 356,445 S371 (c)(1), * cited by examiner (2), (4) Date: Aug. 12, 2008 Primary Examiner — Sang Nguyen (87) PCT Pub. No.: WO2006/013287 (74) Attorney, Agent, or Firm — Blank Rome LLP PCT Pub.
    [Show full text]
  • Impact of Nanotechnology on New Instrumentation
    Impact of Nanotechnology on New Instrumentation Barbara Foster The Microscopy & Imaging Place, Inc Microscopy/Microscopy Education (MME) www.MicroscopyEducation.com [email protected] • Foster, B., "Focus on Microscopy: Pittcon Gives New Eyes to Chemistry," Am Lab, April 2008 • Foster, B., "Focus on Microscopy: Faster, Smaller, More Economical Imaging Tools at SPIE 2007, The Effect of Nanotechnology,“ Am Lab News, Nov/Dec 2007 • Caveats: No commercial interest Apologies to anyone we missed (PDFs of articles and this paper available in The Library at www.MicroscopyEducation.com) 2 Three key topics • Desktop SEMs – a new product category • Collision between spectroscopy & imaging • Extension of light microscopy into the nano world 3 General trends • Smaller footprints • Simpler design • Easier to use Lab-to-Fab/research to routine transition Ideal for students Routine work: QA/QC, quick check in manufacturing • Expanded analytical capabilities •Muchmore economical 4 Desktop SEMs 1. Evex MiniSEM 2. FEI Phenom 3. Hitachi T1000* 4. Jeol/Nikon NeoScope 5. NovelX MySEM 5 Desktop SEMs– a new product category • Fills the magnification gap between LM and SEM LM – up to about 1000x useful magnification EM – 20,000X and higher • Adds 3-dimensionality L: Light microscopy, Diatome R: SEM, Diatome (14,000x) Images courtesy: FEI 6 Desktop SEMs • Magnifications from 20x to 120,000x • 3D imaging • Easy to use Great for students, quick checks in QA/QC • Economical $50-80K, on average No need for highly trained operators or special EM suites • “Horses for courses” Expect reasonable imaging but not all the bells and whistles Clarify your requirements before you purchase 7 Desktop SEMs Evex FEI Hitachi JEOL/Nikon NovelX MiniSEM Phenom T1000 NeoScope MySEM Mag.
    [Show full text]
  • Focus on Microscopy Faster, Smaller, More Economical Imaging Tools at SPIE 2007: the Effect of Nanotech by Barbara Foster
    Volume 39, Number 20 Focus on Microscopy Faster, Smaller, More Economical Imaging Tools at SPIE 2007: The Effect of Nanotech by Barbara Foster Nanotechnology is having a big effect highlights some of the new technolo- trum from 280 to 720 nm, and provides on imaging equipment. In semiconduc- gies on display during the SPIE exhibit a 20,000-fold light boost. Fast image tor parlance, it is driving a strong “lab to that embody this trend. collection is only part of the story, how- fab” transition: Equipment once found ever. The second half is the challenge of only in the research and development Cameras lead the way, from transferring a quarter of a megabyte of laboratory is now finding its way into data that is being collected 2000 times production. While the more sophisti- 60 to 2000 fps per second. Photron’s solution is 2.6 GB cated laboratory versions retain their Collecting low-light-level images at high of onboard recording memory, enabling role for high precision and applicability speed has long been a challenge for cam- a recording time of 8.2 sec for 1000 fps. to multiple applications, the production era manufacturers. Historically, shutters versions are becoming faster, smaller, on the cameras have been left open to sleeker, and more economical. collect images from dim samples over a New ways to image surface period of time and integrate the result- texture, profi les, and This trend was highly visible at the ing data. By its very nature, however, recent International Society for Opti- this process is slow, and longer integra- roughness cal Engineering (SPIE) meeting, held tion times also result in the collection In the crowded and surging field of August 26–30, 2007, in San Diego, of more stray light and electronic noise.
    [Show full text]
  • Microscopes UMDNS #: 12536 Date of Creation: October 29, 2015 Creator: Complied by Cassandra Stanco for Engineering World Health (EWH)
    Equipment Packet: Microscopes UMDNS #: 12536 Date of Creation: October 29, 2015 Creator: Complied by Cassandra Stanco for Engineering World Health (EWH) Equipment Packet Contents: This packet contains information about the operation, maintenance, and repair of laboratory microscopes. Part I: External From the Packet: 1. An Introduction to Microscopes: PowerPoint Part II: Included in this Packet: 1. Operation and Use: a. Introduction to Optical Microscopes (p. 3-12) b. Operation and Use of Microscopes (p. 13-20) 2. Diagrams and Schematics: a. Figure 1: Diagram of a Light Microscope (p. 22) b. Figure 2: Diagram and Parts of a Compound Light Microscope (p. 23) 3. Preventative Maintenance and Safety: a. Microscope Preventative Maintenance (p. 25) 4. Troubleshooting and Repair: a. Microscope Repair and Troubleshooting Flowchart (p. 27-29) 5. Resources for More Information a. Resources for More Information (p. 31) b. Bibliography (p. 32) * * * * 1.*Operation*and*Use*of*Microscopes* * * * Featured*in*this*Section:* * * * WHO.)“Learning)Unit)6:)The)Microscope.”)From)the)Publication:)Basic(Malaria(Microscopy:(part(1.(A( Learner’s(Guide.)WHO:)Switzerland)(2010),)p.)37_44.)Retrieved)from:) http://apps.who.int/iris/bitstream/10665/44208/1/9789241547826_eng.pdfhttp://apps.who. int/iris/bitstream/10665/44208/1/9789241547826_eng.pdf) * * * ) * * * * Optical microscope 1 Optical microscope The optical microscope, often referred to as the "light microscope", is a type of microscope which uses visible light and a system of lenses to magnify images of small samples. Optical microscopes are the oldest and simplest of the microscopes. Digital microscopes are now available which use a CCD camera to examine a sample, and the image is shown directly on a computer screen without the need for optics such as eye-pieces.
    [Show full text]
  • Emerging Optical Nanoscopy Techniques
    Nanotechnology, Science and Applications Dovepress open access to scientific and medical research Open Access Full Text Article REVIEW Emerging optical nanoscopy techniques Paul C Montgomery Abstract: To face the challenges of modern health care, new imaging techniques with subcel- Audrey Leong-Hoi lular resolution or detection over wide fields are required. Far field optical nanoscopy presents many new solutions, providing high resolution or detection at high speed. We present a new Laboratoire des Sciences de l’Ingénieur, de l’Informatique et de classification scheme to help appreciate the growing number of optical nanoscopy techniques. l’Imagerie (ICube), Unistra-CNRS, We underline an important distinction between superresolution techniques that provide improved Strasbourg, France resolving power and nanodetection techniques for characterizing unresolved nanostructures. Some of the emerging techniques within these two categories are highlighted with applica- tions in biophysics and medicine. Recent techniques employing wider angle imaging by digital holography and scattering lens microscopy allow superresolution to be achieved for subcellular For personal use only. and even in vivo, imaging without labeling. Nanodetection techniques are divided into four subcategories using contrast, phase, deconvolution, and nanomarkers. Contrast enhancement is illustrated by means of a polarized light-based technique and with strobed phase-contrast Video abstract microscopy to reveal nanostructures. Very high sensitivity phase measurement using interfer-
    [Show full text]
  • The Joint Nanotec 04/ GDR-E Meeting Has Been Organized Under the Authority of the CNRS (Centre National De La Recherche Scientifique) and the University of Nantes
    The joint Nanotec 04/ GDR-E meeting has been organized under the authority of the CNRS (Centre National de la Recherche Scientifique) and the University of Nantes. Their contribution is greatly acknowledged. We would like also to thank the Institutions and the companies for their financial support: - Conseil Régional des Pays de la Loire - Conseil Général de Loire Atlantique - DGA (Direction générale pour l'Armement) - Bruker Biospin SA - Leica Microsystems - Jobin Yvon-Horiba - Nanocyl - ADB (Atlantique Dessin Bureau Saint Nazaire) - Snecma - Timcal - SGL Carbon All the work of the members of the organizing committee has been highly appreciated and more specially the contribution of: - Annie Simon (secretary) - Jean-Charles Ricquier (Web Master) - Jean-Pierre Buisson (abstract book) Serge Lefrant Local chairman 4th meeting NanoteC 04 Batz-sur-Mer, 2004 October 10-13 NANOTEC 04 PLANNING 4th meeting NanoteC 04 Batz-sur-Mer, 2004 October 10-13 Sunday, October 10 13h 30 – 17h 45 Registration 17h 45 NanoteC’04 Welcome 18h 00 – 19h 30 : Plenary Lectures (PL) Chairman : F. Béguin 18h 00 – 18h 45 : Invited Large scale synthesis, selective fabrication and applications of carbon nanotubes Morinobu Endo 18h 45 – 19h 30 : Invited New Directions of Carbon Nanotube Science : Importance of Defects and Doping M. Terrones, J.-C. Charlier, V. Meunier, E. Hernández, J. Rodríguez-Manzo, F. López-Urías, A.H. Romero, M. Reyes-Reyes, M. S. Dresselhaus, H. Terrones 19h 30 Welcome Party 20h 00 – 21h 30 Dinner : “Buffet de la Presqu’ile” Monday, October 11 8h 30 – 10h 30 : Session O1 Control and Synthesis of Nanomaterials (I) Chairman : S. Lefrant 8h 30 – 9h 00 : Invited Plasma technology: from carbon blacks to fullerenes and nanotubes E.
    [Show full text]
  • Study of Optical Metallurgical Microscope Pdf
    Study of optical metallurgical microscope pdf Continue INDIAN INSTITUTE OF TECHNOLOGY KANPUR Division of Materials and Metallurgical Engineering Virtual Laboratories for Thermal Processing and Materials Characteristics Experiment 1 Microstructure Observation in Light-Optical Microscope Targets Of this laboratory is (i) familiarized with the functioning of the metallurgical microscope, and (ii) observe and interpret the microstructures of these samples. The theory of the microscope function is to turn an object into an image that usually increases to varying degrees. There are many complex techniques (such as electron microscopy) available to perform this transformation. However, the principles involved are just like those developed for light microscopes as far back as 4 centuries ago. The basic concept of any visualization system can be understood from the point of view of a light-optical microscope. The simplest optical microscope is a single convex lens or magnifying lens. There are two important classes of optical microscopes: the type of transmission and the type of reflection of microscopes. Optical mechanisms for these two classes of microscopes are shown in drawings 1.1a and 1.1b. The same two types occur in electron microscopy, which leads to the transmission of electron microscopy and scanning electron microscopy. An integral part of the optical microscope is the lighting system, which consists of a light source and a capacitor lens. The purpose of the capacitor is to focus the diverging beam of light from the source on a small portion of the sample (object A) studied. Most light microscopes use a two- lens system: the lens lens and the eyepiece (often referred to as the projector lens).
    [Show full text]
  • Fingering Instabilities in Dewetting Nanofluids
    PHYSICAL REVIEW LETTERS week ending PRL 100, 176102 (2008) 2 MAY 2008 Fingering Instabilities in Dewetting Nanofluids E. Pauliac-Vaujour, A. Stannard, C. P. Martin, M. O. Blunt, I. Notingher, and P.J. Moriarty The School of Physics and Astronomy, The University of Nottingham, Nottingham NG7 2RD, United Kingdom I. Vancea and U. Thiele School of Mathematics, Loughborough University, Leicestershire LE11 3TU, United Kingdom Max-Planck-Institut fu¨r Physik komplexer Systeme, No¨thnitzer Strasse 38, 01187 Dresden, Germany (Received 18 January 2008; published 30 April 2008) The growth of fingering patterns in dewetting nanofluids (colloidal solutions of thiol-passivated gold nanoparticles) has been followed in real time using contrast-enhanced video microscopy. The fingering instability on which we focus here arises from evaporatively driven nucleation and growth in a nano- scopically thin precursor solvent film behind the macroscopic contact line. We find that well-developed isotropic fingering structures only form for a narrow range of experimental parameters. Numerical simulations, based on a modification of the Monte Carlo approach introduced by Rabani et al. [Nature (London) 426, 271 (2003)], reproduce the patterns we observe experimentally. DOI: 10.1103/PhysRevLett.100.176102 PACS numbers: 68.05.Cf, 61.46.Df, 68.08.Bc A remarkable variety of systems in nature form striking substrates. Following solvent evaporation, the nanopar- branched structures which exhibit fractal properties [1,2]. ticles form a broad variety of self-organized patterns on Key examples include river beds [3], snowflakes [4], bac- the surface [15–18]. We carried out the deposition using a terial populations [2], and the ‘‘viscous fingering’’ patterns simple strategy which exploits the affinity of the solvent which form at the interface of fluids of different viscosity with a Teflon ring to form a meniscus of solution [19].
    [Show full text]
  • Optical Microscope.Pdf
    Optical microscope 1 Optical microscope The optical microscope, often referred to as the "light microscope", is a type of microscope which uses visible light and a system of lenses to magnify images of small samples. Optical microscopes are the oldest and simplest of the microscopes. Digital microscopes are now available which use a CCD camera to examine a sample, and the image is shown directly on a computer screen without the need for optics such as eye-pieces. Other microscopic methods which do not use visible light include scanning electron microscopy and transmission electron microscopy. Optical configurations There are two basic configurations of the conventional optical microscope in use, the simple (one lens) and compound (many lenses). Digital microscopes are based on an entirely different system of collecting the reflected light from a sample. Light microscope A simple microscope is a microscope that uses only one lens for magnification, and is the original light microscope. Van Leeuwenhoek's microscopes consisted of a small, single converging lens mounted on a brass plate, with a screw mechanism to hold the sample or specimen to be examined. Demonstrations [1] by British microscopist have images from such basic instruments. Though now considered primitive, the use of a single, convex lens for viewing is still found in simple magnification devices, such as the magnifying glass, and the loupe. Light microscopes are able to view specimens in color, an important advantage when compared with electron microscopes, especially for forensic analysis, where blood traces may be important, for example. History It is difficult to say who invented the compound microscope.
    [Show full text]