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New Occurrence of B Chromosomes in Rheum Tanguticum Maxim. Ex Balf. (Polygonaceae)

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New Occurrence of B Chromosomes in Rheum Tanguticum Maxim. Ex Balf. (Polygonaceae) CARYOLOGIA Vol. 64, no. 3: 320-324, 2011 New occurrence of B chromosomes in Rheum tanguticum Maxim. ex Balf. (Polygonaceae) HU YANPING, LI WANG and YI LI* Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, Qinghai, China. E-mail HU YANPING: [email protected]. Abstract — A cytogenetic study was conducted on Rheum tanguticum populations from Huangnan Tibetan Au- tonomous Prefecture, China. The results revealed the chromosome numbers 2n=22 and the karyotype formulae are 2n=22=22m or 2n=22=2sm+20m, belonging to Stebbins 1A type. However, it was interesting to observe metaphases with variable B chromosomes (1, 2, 3, 4, 6, 7) in a few specimens. For the fi rst time, the occurrence of B chromosomes was reported in natural populations of R. tanguticum. The number of B chromosomes in R. tanguticum varied not only among individuals but also within the same root tip. Key words: B chromosomes; karyotype; Rheum tanguticum Maxim. ex Balf. INTRODUCTION chromosome, which is called the supernumerary chromosome, is additional and mostly dispensa- The genus Rheum, a member of the fam- ble chromosome found in nearly 1300 plants and ily Polygonaceae, including about 60 species, over 500 animals, as well as 10 species of fungi is mainly distributed in the mountainous and (JONES 1975; JONES and REES 1982; MIAO et al. desert regions of the Qinghai-Tibetan Plateau 1991; JONES 1995; LECLAIR et al. 1996; CAMACHO areas and subtropical Asia (LI 1998). Rheum tan- et al. 2000; JONES and DIEZ 2004; CAMACHO 2005; guticum Maxim. ex Balf., the root and rhizome S-GARMAROODI and TAGA 2007; VOLTOLIN et al. of which is commonly called rhubarb, is a widely 2010). B chromosomes share a number of com- used traditional Chinese medicinal herb and one mon features as follows (BEUKEBOOM 1994; JONES of the important ingredients in Chinese tradi- and HOUBEN 2003; JONES et al. 2008): (1) they are tional prescriptions (YANG et al. 2001; WANG often morphologically distinct, usually smaller and REN 2009). Rhubarb has been used to treat than the autosomes (standard A chromosomes); various syndromes caused by the obstruction of (2) they are generally heterochromatic and vary in blood circulation (such as dysmenorrhoea, hy- number among individuals from the same popu- permenorrhea, hematemesis, lower abdominal lation; (3) their inheritance is non-Mendelian; (4) pain, etc), jaundice, diarrhea, as well as constipa- they are regarded as inert material, although a tion (KOMATSU et al. 2006; LI et al. 2006; ZHANG few active genes in some B chromosomes have ac- et al. 2009; CHINESE PHARMACOPOEIA COMMIS- tually been found, especially for ribosomal genes SION 2010). (MIAO et al. 1991; JAMILENA et al. 1994; VANETTEN Cytological data are fundamental to studies et al. 1994; DONALD et al. 1995; JONES 1995; CO- of plant taxonomy, evolution, diversifi cation and VERT et al. 1996; LECLAIR et al. 1996; FUNNELL and breeding (STEBBINS 1971; STACE 2000). The B- VANETTEN 2002); (5) they rarely share any linkage homology with A chromosomes; (6) they are rich in highly repetitive sequences. Previous chromosome studies on Rheum showed a consistent basic chromosome number (X=11) (DARLINGTON and WYLIE 1955; HU et al. *Corresponding author: phone (fax): +86 971 6101454; 2007; LIU et al. 2010), without any B chromo- e-mail: [email protected] somes reported. In this paper, the karyotypes of NEW OCCURRENCE OF B CHROMOSOMES IN RHEUM TANGUTICUM MAXIM. EX BALF. (POLYGONACEAE) 321 wild R. tanguticum in their genuine producing for observation. The chromosome number was area were carried out and B chromosomes were determined under a light microscope (Nikon fi rstly reported. Eclipse 50i) and photomicrographs were made with an optical camera (Nikon DS-U2). The metaphase chromosomes of at least 5 cells of MATERIAL AND METHODS different plant roots were measured. Karyotype analysis was followed by LI and CHEN (1985), Seeds of R. tanguticum were collected and karyotype classifi cation was performed as from two populations (MX: 101°56′38.1″E, the method described by STEBBINS (1971). 35°16′35.6″N; NX: 100°51′6.4″E, 35°11′27.3″N) of Huangnan Tibetan Autonomous Prefecture, Qinghai, China. They were germinated in petri RESULTS dishes lined on moist fi lter paper. The root tips were pretreated in distilled water at 0°C for 24h Our results showed a diploid chromosome when they grow up to a length of 1cm, then fi xed number equal to 2n=22 and the karyotype was in Carnoy (absolute alcohol: glacial acetic acid mainly composed of metacentric (m) chro- 3:1) at 4°C for 24h, and transferred to 70% etha- mosomes and submetacentric (sm) chromo- nol for storage at 4°C until required. The fi xed somes. Karyotypic formulae were 2n=22=22m roots were hydrolyzed in 1mol/L hydrochloric for MX population (Figure 1A and 1A`) and acid at 60°C for 10 min, then washed with dis- 20=22=2sm+20m for NX population (Figure 1B tilled water. Finally, samples were stained with and 1B`), both belonging to 1A type according carbol fuchsin and squashed in 45% acetic acid to STEBBINS (1971). However, in some specimens Fig. 1 — Cytological features of R. tanguticum. A-B, Chromosomes of metaphases. Aʹ–Bʹ, Karyotypes. A, Aʹ, MX population. B, Bʹ, NX population. Bars=10µm. TABLE 1 — The frequency of B chromosomes in R. tanguticum from Huangnan Tibetan Autonomous Prefecture. 0B 1B 2B 3B 4B 6B 7B Analyzed cells MX 6743547921 183 NX 17662720 40 Total number of cells 84 49 60 9 16 4 1 223 % 37.67 21.97 26.91 4.04 7.17 1.79 0.45 100 322 YANPING, WANG and LI it was also possible to observe metaphases with (YANG and GONG 1995), Schismatoglottis irrorata 1, 2, 3, 4, 6 or 7 B chromosomes (Table 1, Figure (OKADA 1992) and Crepis capillaris (BROWN and 2). Among all the cells that analyzed, the propor- JONES 1976; MALUSZYNSKA 1997; LEACH et al. tion of 1B and 2B chromosomes was similar and 2005). Additionally, in Polygonaceae, only two high (Table 1). Only one cell was found contain- Rumex species including Rumex thyrsifl orus and ing 7B chromosomes (fi gure 2C). Besides, most Rumex acetosa were found with B chromosomes of the B chromosomes in R. tanguticum were (ZUK 1969; PARKER and WILBY 1989). In Rumex small metacentric chromosomes. thyrsifl orus the B chromosome was large, and equaled in size to the X sex chromosome (ZUK 1969). However, B chromosomes in Rumex ac- DISCUSSION etosa were small telocentric and the number of B chromosomes varied between cells, tissues B chromosomes have already been described and organs of the same plant (PARKER and WILBY in many other perennial plants, such as Allium 1989). Nevertheless, there are no reports about B prattii (XUE et al. 2000), Aconitum tuguancunense chromosomes in Rheum species, and this repre- Fig. 2 — Metaphases of R. tanguticum with B chromosomes. Arrows indicate B chromosomes. Bars=10µm. NEW OCCURRENCE OF B CHROMOSOMES IN RHEUM TANGUTICUM MAXIM. EX BALF. (POLYGONACEAE) 323 sents the fi rst occurrence reported for this genus. macopoeia of the People’s Republic of China. p. 22- According to JONES (1995), B chromosomes 23. China Medical Science Press, Beijing. are restricted to outbreeders and the probable COVERT S.F., ENKERL I.J., MIAO V.P.W. and VANETTEN reason for this is that selfi ng would not facilitate H.D., 1996 — A gene for maackiain detoxifi cation from a dispensable chromosome of Nectria haema- the spread and accumulation in populations by tococca. Molecular and General Genetics, 251: mitotic drive. Therefore, the appearance of B 397-406. chromosomes in R. tanguticum further confi rmed DARLINGTON C.D. and WYLIE A.P., 1955 — Chromo- the outcrossing breeding system of R. tanguticum. some atlas of fl owering plants. George Allen and This was consistent with our previous studies by Unwin Ltd., London. molecular markers (HU et al. 2010). DONALD T.M., LEACH C.R., CLOUGH A. and TIMMIS The number of B chromosomes in R. tan- J.N., 1995 — Ribosomal RNA genes and the B guticum varied not only among individuals but chromosome of Brachycome dichromosomatica. Heredity, 74: 556-561. also within the same root tip. This variation of FUNNELL D.L. and VANETTEN H.D., 2002 — Pisatin B chromosomes was also found in other species demethylase genes are on dispensable chromo- like Aconitum tuguancunense (YANG and GONG somes while genes for pathogenicity on carrot and 1995), Schismatoglottis irrorata (OKADA 1992), ripe tomato are on other chromosomes in Nectria Allium mairei (CHEN et al. 2005) and Ornithoga- haematococca. Molecular Plant-Microbe Interac- lum Caudatum (CAI et al. 2007). tions, 15: 840-846. Although the presence of B chromosomes HU Y.P., XIE X.L., WEN Q., ZHAO X.D., WANG L. and LI Y., 2007 — Studies on karyotypes of fi ve popula- has been discovered in different populations tions of Rheum tanguticum (Polygonaceae). Acta of R. tanguticum, further studies on B chromo- Botanica Yunnanica, 29: 429-433. somes is still required in order to provide a bet- HU Y.P., WANG L., XIE X.L., YANG J., LI Y. and ZHANG ter understanding of chromosomal evolution in H.G., 2010 — Genetic diversity of wild popula- Rheum species. tions of Rheum tanguticum endemic to China as revealed by ISSR analysis. Biochemical Systemat- Acknowledgements — This work was supported ics and Ecology, 38: 264-274. by West Light Foundation of the Chinese Academy JAMILENA M., REJON C.R. and REJON M.R., 1994 — A of Sciences (2009), Natural Science Foundation of molecular analysis of the origin of the Crepis-capil- Qinghai Province of China (2011-Z-921Q) and Fron- laris B-chromosome.
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