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GE Healthcare Affinity Chromatography Affinity Chromatography – Vol. 1: Antibodies Vol. www.gelifesciences.com GE, GE monogram, ÄKTA, ÄKTApilot , ÄKTAprocess, Amersham, AxiChrom, Biacore, BioProcess, Capto, Cy, ECL, ECL Plex, ECL Select, ExcelGel, GraviTrap, HiLoad, HiPrep, HiScale, HiScreen, HiTrap, Hybond, MabSelect, MabSelect SuRe, MabSelect Xtra, MabTrap, MidiTrap, MiniTrap, MultiTrap, PhastGel, PhastSystem, PreDictor, PrimeView, Protran, ReadyToProcess, Sephadex, Sepharose, SpinTrap, Superdex, Superloop, Tricorn, and UNICORN are trademarks of General Electric Company. Coomassie is a trademark of Thermo Fisher Scientific LLC. IPEGAL is a trademark of Rhodia. RoboColumn is a trademark of Atoll GmbH. TEFZEL is a trademark of E.I. du Pont de Nemours and Company. Tween is a trademark of Croda Group of Companies. All other third-party trademarks are the property of their respective owners. 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Use of this material to perform services for a fee for third parties, including contract research and drug screening. Björkgatan 30 If you require a commercial license to use the Cy and CyDye trademarks please contact [email protected]. 751 84 Uppsala © 2014–2016 General Electric Company. Previously published Jan. 2014. Sweden All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information. GE Healthcare UK Ltd, Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK GE Healthcare Bio-Sciences Corp. 100 Results Way, Marlborough, MA 01752, USA GE Healthcare Dharmacon, Inc., 2650 Crescent Dr., Lafayette, CO 80026, USA HyClone Laboratories, Inc., 925 W 1800 S, Logan, UT 84321, USA GE Healthcare Europe GmbH, Munzinger Strasse 5, D-79111 Freiburg, Germany imagination atGE workHealthcare Japan Corporation, Sanken Bldg. 3-25-1, Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan imagination at work For local office contact information, visit www.gelifesciences.com/contact 18103746 AF 04/2016 Handbooks from GE Healthcare Life Sciences For more information refer to www.gelifesciences.com/handbooks GE Healthcare GE Healthcare GE Healthcare Affinity Chromatography Life Sciences High-throughput Process Life Sciences Protein Sample Vol. 1: Antibodies High-throughput Development with Preparation Process Development with PreDictor™ Plates 18103746 Principles and Methods PreDictor Plates Handbook Principles and Methods 28988741 28940358 Protein Sample Affinity Chromatography Preparation Vol. 1: Antibodies Handbook GE Healthcare GE Healthcare GE Healthcare Affinity Chromatography Life Sciences Hydrophobic Interaction Life Sciences Purifying Challenging Vol. 2: Tagged Proteins and Reversed Phase Proteins 18114275 Chromatography Principles and Methods Principles and Methods 28909531 Hydrophobic Interaction Purifying and Reversed Phase 11001269 Challenging Proteins Chromatography Affinity Chromatography Principles and Methods Principles and Methods Vol. 2: Tagged Proteins GE Healthcare GE Healthcare GE Healthcare Affinity Chromatography Life Sciences Imaging Life Sciences Size Exclusion Vol. 3: Specific Groups of 473 532 635 650 685 785 Principles and Methods Chromatography Laser UV IR epi Biomolecules trans 29020301 Principles and Methods 312 CCD UV IR W 365 460 520 630 710 18102229 epi 18102218 Imaging Principles and Methods Size Exclusion Chromatography Affinity 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Blotting Biacore Handbook Principles and Methods Principles and Methods Sensor Surface Handbook BR100571 18114062 28999897 Microcarrier Cell Culture Western Blotting Principles and Methods Principles and Methods GE Healthcare GE Healthcare GE Healthcare Life Sciences Cell Separation Media Life Sciences Multimodal Life Sciences 2-D Electrophoresis using Methodology and Chromatography Immobilized pH Gradients Applications Handbook Principles and Methods 18111569 29054808 80642960 Cell Separation Media 2-D Electrophoresis Principles and Methods Methodology and applications Multimodal Chromatography Handbook imagination at work GE Healthcare GE Healthcare Life Sciences GST Gene Fusion System Life Sciences Nucleic Acid Sample Handbook Preparation for 18115758 Downstream Analyses Principles and Methods Nucleic Acid Sample GST Gene Preparation for 28962400 Fusion System Downstream Analyses Handbook Principles and Methods Affinity Chromatography Vol. 1: Antibodies Contents Introduction ........................................................................................................................................7 Symbols ...............................................................................................................................................................................8 Common acronyms and abbreviations ...............................................................................................................8 Chromatography terminology ................................................................................................................................. 9 Chapter 1 Antibody structure, classification, and production ...................................................................13 Native sources ...............................................................................................................................................................13 Immunoglobulins ..................................................................................................................................................13 IgY immunoglobulin ............................................................................................................................................15 Antibody fragments ............................................................................................................................................15 Polyclonal antibodies ..........................................................................................................................................16 Monoclonal antibodies ......................................................................................................................................16 Genetically engineered sources ............................................................................................................................17 Antibody fragments ............................................................................................................................................17 Recombinant antibodies ...................................................................................................................................17 References .......................................................................................................................................................................18 Chapter 2 Sample preparation ........................................................................................................................19 Sources and their associated contaminants ..................................................................................................19 Extraction of recombinant antibodies and antibody fragments ...........................................................20 Centrifugation and filtration ............................................................................................................................22 Sample preparation before purification ............................................................................................................23 Removal of specific impurities before purification ...............................................................................23
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