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Affinity Chromatography-Biomolecules GE Healthcare Affinity Chromatography Affinity Chromatography – Vol. 3: Specific Groups of Biomolecules 3: Specific Groups Vol. www.gelifesciences.com GE, GE monogram, Amersham, ÄKTA, Biacore, BioProcess, Capto, Cy, CyDye, ECL, ECL Plex, ECL Select, ExcelGel, GSTrap, HiPrep, HiScale, HiScreen, HiTrap, ImageQuant, Hybond, MiniTrap, MabSelect, MiniTrap, MultiTrap, PhastGel, PhastSystem, PrimeView, Protran, Sephadex, Sepharose, SPARTAN, SpinTrap, Superdex, Typhoon, Tricorn, UNICORN, and Whatman are trademarks of General Electric Company. Coomassie is a trademark of Thermo Fisher Scientific LLC. Neutravidin is a trademark of Pierce Biotechnology, Inc. Nonidet is a trademark of Air Products and Chemicals, Inc. Pefabloc is a trademark of DSM IP Assets B.V. Tween is a trademark of Croda Group of Companies. All other third-party trademarks are the property of their respective owners. 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GE Healthcare UK Ltd, Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK GE Healthcare Bio-Sciences Corp. 100 Results Way, Marlborough, MA 01752, USA GE Healthcare Dharmacon, Inc., 2650 Crescent Dr., Lafayette, CO 80026, USA HyClone Laboratories, Inc., 925 W 1800 S, Logan, UT 84321, USA GE Healthcare Europe GmbH, Munzinger Strasse 5, D-79111 Freiburg, Germany imagination atGE work Healthcare Japan Corporation, Sanken Bldg. 3-25-1, Hyakunincho, Shinjuku-ku, Tokyo 169-0073, Japan imagination at work For local office contact information, visit www.gelifesciences.com/contact imagination at work 18102229 AF 03/2016 Handbooks from GE Healthcare Life Sciences For more information refer to www.gelifesciences.com/handbooks GE Healthcare GE Healthcare GE Healthcare Affinity Chromatography Life Sciences GST Gene Fusion System Life Sciences Nucleic Acid Sample Vol. 1: Antibodies Handbook Preparation for 18103746 18115758 Downstream Analyses Principles and Methods Nucleic Acid Sample GST Gene Preparation for 28962400 Affinity Chromatography Fusion System Downstream Analyses Vol. 1: Antibodies Handbook Principles and Methods GE Healthcare GE Healthcare GE Healthcare Affinity Chromatography Life Sciences High-throughput Process Life Sciences Protein Sample Vol. 2: Tagged Proteins High-throughput Development with Preparation Process Development with PreDictor™ Plates 18114275 Principles and Methods PreDictor Plates Handbook Principles and Methods 28988741 28940358 Protein Sample Affinity Chromatography Preparation Vol. 2: Tagged Proteins Handbook GE Healthcare GE Healthcare GE Healthcare Affinity Chromatography Life Sciences Hydrophobic Interaction Life Sciences Purifying Challenging Vol. 3: Specific Groups of and Reversed Phase Proteins Biomolecules Chromatography Principles and Methods 18102229 Principles and Methods 28909531 Hydrophobic Interaction Purifying and Reversed Phase 11001269 Challenging Proteins Chromatography Affinity Chromatography Principles and Methods 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Blotting Biacore Handbook Mononuclear Cells Principles and Methods Sensor Surface Handbook BR100571 Methodology and 28999897 Applications Isolation of 18115269 mononuclear cells Methodology and applications Western Blotting Principles and Methods GE Healthcare GE Healthcare GE Healthcare Life Sciences Cell Separation Media Life Sciences Microcarrier Cell Culture Life Sciences 2-D Electrophoresis using Methodology and Principles and Methods Immobilized pH Gradients Applications 18114062 Principles and Methods 18111569 80642960 Cell Separation Media 2-D Electrophoresis Principles and Methods Methodology and applications Microcarrier Cell Culture Principles and Methods GE Healthcare GE Healthcare Life Sciences Size Exclusion Life Sciences Multimodal Chromatography Chromatography Principles and Methods Handbook 18102218 29054808 Size Exclusion Chromatography Multimodal Principles and Methods Chromatography Handbook imagination at work Affinity Chromatography Vol. 3: Specific Groups of Biomolecules Content Introduction.........................................................................................................................................9 Symbols .............................................................................................................................................................................10 Common acronyms and abbreviations .............................................................................................................10 Chapter 1 Principles of affinity chromatography ........................................................................................ 13 Components of an affinity chromatography medium ...............................................................................15 Matrix ..........................................................................................................................................................................15 Ligand .........................................................................................................................................................................16 Spacer arms ............................................................................................................................................................17 Chapter 2 Affinity chromatography in practice ........................................................................................... 19 Selection of chromatography media .................................................................................................................19 Selection of format ......................................................................................................................................................19 Selection of equipment ...........................................................................................................................................20 Selection of purification method ..........................................................................................................................21 Preparation of sample and buffers ..............................................................................................................21 Flow rates .................................................................................................................................................................21 Equilibration .............................................................................................................................................................21 Sample application and wash ........................................................................................................................21 Elution .........................................................................................................................................................................22
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