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Biomolecules Content Introduction.........................................................................................................................................9 Symbols .............................................................................................................................................................................10 Common acronyms and abbreviations .............................................................................................................10 Chapter 1 Principles of affinity chromatography ........................................................................................ 13 Components of an affinity chromatography medium ...............................................................................15 Matrix ..........................................................................................................................................................................15 Ligand .........................................................................................................................................................................16 Spacer arms ............................................................................................................................................................17 Chapter 2 Affinity chromatography in practice ........................................................................................... 19 Selection of chromatography media .................................................................................................................19 Selection of format ......................................................................................................................................................19 Selection of equipment ...........................................................................................................................................20 Selection of purification method ..........................................................................................................................21 Preparation of sample and buffers ..............................................................................................................21 Flow rates .................................................................................................................................................................21 Equilibration .............................................................................................................................................................21 Sample application and wash ........................................................................................................................21 Elution .........................................................................................................................................................................22