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(11) EP 3 156 793 A1

(12) EUROPEAN PATENT APPLICATION

(43) Date of publication: (51) Int Cl.: 19.04.2017 Bulletin 2017/16 G01N 31/22 (2006.01)

(21) Application number: 15190253.3

(22) Date of filing: 16.10.2015

(84) Designated Contracting States: (72) Inventors: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB • TOMCZAK, Douglas Charles GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO Trumbull, Connecticut, 06611 (US) PL PT RO RS SE SI SK SM TR • AGRAWAL, Nikhil Designated Extension States: Trumbull, Connecticut, 06477 (US) BA ME • MARRIOTT, Robert Edward Designated Validation States: Bebington, Wirral, Merseyside, MA CH63 3JW (GB) • GIBSON, Oliver Lawrence (71) Applicants: Leeds, West Yorkshire, LS14 2AR (GB) • Unilever PLC London, Greater London EC4Y 0DY (GB) (74) Representative: McHugh, Paul Edward Designated Contracting States: Unilever PLC CY GB IE MT Unilever Patent Group • Unilever N.V. Colworth House 3013 AL Rotterdam (NL) Sharnbrook Designated Contracting States: Bedford AL AT BE BG CH CZ DE DK EE ES FI FR GR HR Bedfordshire MK44 1LQ (GB) HU IS IT LI LT LU LV MC MK NL NO PL PT RO RS SE SI SK SM TR

(54) TESTING METHOD FOR SPECIFIC INGREDIENTS IN ANTIPERSPIRANT AND HUMECTANT COSMETIC COMPOSITIONS

(57) A method of demonstrating the potential for a um-containingantiperspirant salt and/or moisturiser, said moisturisation and/or antiperspirancy effect from a cos- detector substance generating a visible colour change metic composition, said method comprising contacting to indicate the presence of aluminium-containing antiper- the composition with a detector substance for alumini- spirant salt and/or moisturiser. EP 3 156 793 A1

Printed by Jouve, 75001 PARIS (FR) 1 EP 3 156 793 A1 2

Description example, with reference to the following figures in which:

[0001] The invention concerns a method for demon- FIGURE 1 is a photograph of three cosmetic com- strating the potential for a moisturisation and/or antiper- positions and a substrate prior to application, spirancy effect from a cosmetic composition. 5 [0002] Cosmetic compositions contain a number of dif- FIGURE 2 is a photograph of the three cosmetic ferent ingredients to perform various functions. Some compositions immediately after application to the cosmetic compositions are intended to provide a mois- substrate, turisation and/or antiperspirancy effect to the user. It is beneficial to be able to demonstrate the potential for a 10 FIGURE 3 is a photograph three minutes after ap- moisturisation and/or antiperspirancy effect from a cos- plication of the three cosmetic compositions to the metic composition. The present invention addresses that substrate, goal. [0003] According to the present invention, there is pro- FIGURE 4 is a photograph showing a positive result vided a method of demonstrating the potential for a mois- 15 from the application of a cosmetic composition to a turisation and/or antiperspirancy effect from a cosmetic test substrate, composition, said method comprising contacting the composition with a detector substance for aluminium- FIGURE 5 is a photograph of a negative result of the containing antiperspirant salt and/or moisturiser, said de- application of a comparative cosmetic product to a tector substance generating a visible colour change to 20 test substrate, indicate the presence of aluminium-containing antiper- spirant salt and/or moisturiser. FIGURE 6 is a photograph of PEG-8 applied to a [0004] Preferably, the moisturiser is a humectant. test substrate under ambient conditions, [0005] Conveniently, the step of contacting the com- position with the detector substance comprises the ap- 25 FIGURE 7 is a photograph of PEG-8 applied to a plication of the composition to a fibrous substrate com- test substrate within a desiccator, prising the detector substance. [0006] Advantageously, the fibrous substrate is paper. FIGURE 8 is a photograph showing the results of [0007] Preferably, the cosmetic composition is an an- the application of the surfactant Tween-20 and PEG- tiperspirant composition. 30 8, [0008] Advantageously, the cosmetic composition is in the form of a stick, aerosol, roll-on or cream. FIGURE 9 is a photograph showing the results of [0009] Preferably, the cosmetic composition is in the the application of two cosmetic compositions to a form of a stick test substrate with two different dilutions of a test [0010] Conveniently, the detector substance is for an 35 substance, and aluminium-containing antiperspirant salt. [0011] Advantageously, the detector substance com- FIGURE 10 is a photograph showing the results of prises Chromeazurol S or xylenol orange. the application of four cosmetic compositions to test [0012] Preferably, the detector substance is for a hu- substrates for the detection of glycerol and alumini- mectant. 40 um. [0013] Conveniently, the detector substance is for glycerol. [0019] Aluminium compounds are used in many cos- [0014] Advantageously, the detector substance com- metic compositions to provide antiperspirancy effects. It prises enzymes. would be beneficial to be able to demonstrate such an- [0015] Preferably, the detector substance is for poly- 45 tiperspirancy effects in a simple and quick way. In one propylene glycol or polyethylene glycol. aspect, the present invention relates to a method for dem- [0016] Conveniently, the detector substance compris- onstrating the potential for an antiperspirancy effect from es an inorganic salt and a pH indicator dye. a cosmetic composition comprising contacting the com- [0017] According to the invention, there is provided a position with a detector substance for aluminium contain- method of distinguishing a cosmetic composition having 50 ing antiperspirant salt, said detector substance generat- a moisturisation and/or antiperspirancy effect from an- ing a visible colour change to indicate the presence of other cosmetic composition, said method comprising an aluminium-containing antiperspirant salt. contacting the composition with a detector substance for [0020] To perform the method, the cosmetic composi- aluminium-containing antiperspirant salt and/or moistur- tion, which may for example be in the form of a stick, is iser, said detector substance generating a visible colour 55 contacted with the detector substance. This may be per- change to indicate the presence of aluminium-containing formed, for example, by mixing the composition with the antiperspirant salt and/or moisturiser. detector substance on a substrate surface, such as in a [0018] The invention will now be described, by way of container or vessel. Alternatively, the detector substance

2 3 EP 3 156 793 A1 4 may be present in or on a fibrous substrate, and the com- able from the Sigma Aldrich company. This particular re- position may be applied to the fibrous substrate. So, for agent contains a number of enzymes and other compo- example, the composition can be applied to the fibrous nents which can provide a visible colour change as an material, for example by applying a smear, and then the indication for the presence of glycerol in a test composi- substrate can be monitored to see whether there is a 5 tion. For this particular reagent, a colour change to purple colour change. indicates a positive result. [0021] Advantageously the method may, at least with [0027] In one aspect, a fibrous substrate can be used respect to the detection of moisturiser, be carried out in the test which may be, for example, paper or card. The ambient humidity conditions: in particular at relative hu- method can be used for compositions in the form of a midity of less than 80%, more particularly less than 70%. 10 stick and for compositions in non-stick forms, such as In one or more embodiments of particular interest the roll-on, aerosol or cream compositions. This is especially method is carried out at relative humidity of from 20 to the case for methods relating to humectants and antiper- 60%. spirant effects, particularly for glycerol and aluminium [0022] A test for the presence of aluminium can provide salts. a visible colour change to indicate the antiperspirancy 15 [0028] Other preferred moisturisers are triglycerides, potential of a product. A suitable test is the reagent such as those in sunflower seed oil. The present method Chromeazurol S obtainable from the Sigma Aldrich com- also relates to demonstrating the potential for moisturi- pany. For this particular reagent, a colour change from sation from cosmetic compositions containing such mois- orange to purple indicates a positive result. Another suit- turisers. A suitable detector substance is a reagent for able reagent is xylenol orange. 20 the presence of triglycerides. One such reagent is the [0023] Compounds such as some polypropylene gly- Serum Triglyceride Determination Kit available from the cols and polyethylene glycols are used in cosmetic com- Sigma Aldrich company. The kit contains enzymes and positions to provide moisturising properties. These par- other compounds. ticular materials are humectants. In other words, such [0029] The procedure involves enzymatic hydrolysis compounds help to retain moisture when applied to the 25 by lipase of the triglycerides to glycerol and free fatty skin of a user. The method relates to the demonstration acids. The glycerol produced is then measured by cou- of the presence of a range of moisturisers, inlcuding hu- pled enzyme reactions. mectants, such as polyethylene glycols, polypropylene [0030] Triglycerides are first hydrolyzed by lipoprotein glycols, butylene glycols, pentylene glycols, hexylene lipase to glycerol and free fatty acids. Glycerol is then glycols, isomers, dimers and trimers thereof, and other 30 phosphorylated by adenosine-5’-triphosphate (ATP) liquid "hydroxyl" humectants such as diglycerol and pol- forming glycerol-1-phosphate (G-1-P) and adenosine-5’- yglycerol. Preferably, the method involves the detection diphosphate (ADP) in the reaction catalyzed by glycerol of a polyethylene glycol, such as PEG-4, PEG-6, PEG- kinase (GK). G-1-P is then oxidized by glycerol phos- 8 and PEG-10. A preferred humectant is PEG-8. phate oxidase (GPO) to dihydroxy-acetone phosphate 35 [0024] It would be beneficial to be able to provide a (DAP) and hydrogen peroxide (H 2O2). Peroxidase (POD) method of demonstrating the potential for a moisturisa- catalyzes the reaction of H2O2 with 4-aminoantipyrine tion effect from a cosmetic composition comprising the (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisi- application of the composition to a fibrous substrate com- dine (ESPA) to produce a quinoneimine dye that shows prising a detector substance for a moisturiser, said de- an absorbance maximum at 540 nm. The increase in tector substance generating a visible colour change to 40 absorbance at 540 nm is directly proportional to triglyc- indicate the presence of a moisturiser, preferably a hu- eride concentration of the sample. mectant. A preferred method of doing so comprises ap- [0031] In some cases, it is beneficial to dilute or soften plying a cosmetic composition to a fibrous substrate com- the cosmetic composition for use in the method. For ex- prising thedetector substance and monitoringfor a colour ample, the composition may be mixed with a material to change. 45 make it more fluid to facilitate the interaction with the [0025] One preferred method of testing for the pres- detector substance. One material that can be used for ence of a humectant such as PEG-8 is the use of a colour this purpose is a volatile silicone such as cyclopentasi- changing indicating paper. Particularly preferred meth- loxane. ods use indicator papers comprising an inorganic salt [0032] Whilst the invention has been described in re- and a pH indicator dye. The application of a material that 50 lation to a fibrous substrate, the method could be per- contains moisture or a humectant causes a pH change formed without such a substrate. So, for example, the resulting in the colour change which can be observed as cosmetic composition could be mixed with a detector a positive result. A particularly preferred test substrate is substance on another surface or in a vessel or container. "WatorPaper" obtainable from the Macherey-Nagel com- The invention also relates to a method of demonstrating pany (Reference: 90610). 55 the potential for a moisturisation and/or antiperspirancy [0026] Another preferred humectant is glycerol. The effect from a cosmetic composition, said method com- presence of glycerol as humectant can be detected by a prising the mixing of the composition with a detector sub- suitable test, such as the "free glycerol reagent" obtain- stance for aluminium-containing antiperspirant salt

3 5 EP 3 156 793 A1 6 and/or moisturiser, said detector substance generating sition, and there is no colour change (negative result) a visible colour change to indicate the presence of alu- from the Secret Outlast Completely Clean Invisible Solid minium-containing antiperspirant salt and/or moisturiser. cosmetic composition and the Lady Speed Stick Invisible Preferably, the moisturiser is a humectant. Dry Powder Fresh Stick cosmetic composition. The pos- [0033] The method of the invention was exemplified in 5 itive result is believed to be due to the inclusion of PEG- relation to the presence of a humectant. Three different 8 in the Dove Nourished Beauty Stick. The test demon- cosmetic compositions were assessed in the method. strates the potential for a moisturisation effect from the The components of each of the three cosmetic compo- cosmetic composition. sitions that were used are set out below: [0038] The test protocol in general terms is set out be- 10 low, and is described with reference to Figures 4 and 5. • Dove Nourished Beauty Stick [0039] The test comprises taking a piece of the test Aluminium Zirconium Tetrachlorohydrex GLY, Cy- paper from its protective packaging immediately before clopentasiloxane, Stearyl Alcohol, C12-15 Alkyl the test. Before the test, the paper is kept in the packaging Benzoate, PPG-14 Butyl Ether, Hydrogenated Cas- to avoid contact with environmental moisture, which tor Oil, PEG-8, Dimethicone, Fragrance (Parfum), 15 could affect the results. Next, the cosmetic product to be Silica, Polyethylene, Helianthus Annuus (sunflower) tested is used to deposit material on the test substrate. Seed Oil, Steareth-100, BHT, Hydroxyethyl Urea This can either be in the form of a smear, or could also take the form of either text or a picture applied to the • Secret Outlast Completely Clean Invisible Solid surface of the paper. After some time has elapsed, typ- Aluminium Zirconium Tetrachlorohydrex GLY, Cy- 20 ically around three minutes, a visible colour change can clopentasiloxane, Stearyl Alcohol, C12-15 Alkyl be seen for cosmetic compositions having the potential Benzoate, PPG-14 Butyl Ether, Cyclodextrin, Petro- for moisturisation. In particular, a positive test result can latum, Phenyl Trimethicone, Hydrogenated Castor be seen for cosmetic composition containing PEG-8. Oil, Talc, Fragrance, Ozokerite, Behenyl Alcohol, [0040] The method can be performed simultaneously Panthenyl Triacetate, Tocopheryl Acetate, Acetyl 25 with a cosmetic composition which does produce a pos- Glucosamine itive result and also using a cosmetic composition which does not produce a positive result. FIGURE 4 shows the • Lady Speed Stick Invisible Dry Powder Fresh Stick positive result resulting from the application of a cosmetic Aluminium Zirconium Tetrachlorohydrex GLY, composition (Dove) which does contain the humectant Elaeis Guineensis (palm) Kernel Oil, Stearyl Alcohol, 30 PEG-8, whereas FIGURE 5 shows the negative result Cyclomethicone, C12-15 Alkyl Benzoate, PPG-14 from the application of a comparative product which does Butyl Ether, Hydrogenated Castor Oil, Hydrogenat- not contain PEG-8. There is a visible colour change to ed Soybean Oil, PEG-8 Distearate, Fragrance, Hy- indicate a positive result for the cosmetic composition drolyzed Corn Starch, Behenyl Alcohol that does contain the humectant PEG-8. 35 [0041] The "Wator" test paper is known to produce a [0034] Each of the cosmetic compositions were as- positive result in the presence of moisture (water). To sessed according to the method of the invention in rela- assess whether the positive result seen for cosmetic tion to demonstrating the potential for a moisturisation compositions containing a humectant such as PEG-8 is and/or antiperspirancy effect. The cosmetic composi- due to the presence of water, or the attraction of water tions were applied to a fibrous substrate comprising a 40 from ambient environments, a further test was carried detector substance for moisture. In particular, the fibrous out. substrate used in the test was the "Wator" test paper [0042] In the test, two pieces of the test substrate paper obtainable from the Machery-Nagel company. were used. One of the pieces of paper was placed in a [0035] FIGURE 1 shows the three cosmetic composi- desiccator whilst the other was left out on a bench sur- tions and a sheet of the fibrous test substrate prior to45 face, in ambient conditions. To each piece, one drop of application. PEG-8 liquid was added and the paper was subsequently [0036] FIGURE 2 shows the situation where the cos- monitored for any positive reactions. metic compositions, which were each in the form of a [0043] FIGURES 6 and 7 show the result of the test. stick, have been applied to the fibrous substrate by draw- Both papers reacted positively with PEG-8. FIGURE 6 ing the sticks over the surface of the fibrous substrate. 50 shows the positive result from the addition of a drop of FIGURE 2 shows the situation immediately after appli- PEG-8 material to the test paper left open in ambient cation, with a smear of each product being visible on the conditions. FIGURE 7 shows the positive result obtained test substrate. No colour change has yet occurred. from the addition of a drop of PEG-8 liquid to the test [0037] FIGURE 3 shows the situation three minutes paper which was then kept within a desiccator. The test after application. Only one of the three cosmetic compo- 55 results indicate that the positive colour change is due to sitions has produced a visible colour change, where the the addition of the PEG-8 liquid itself, rather than the streak has turned blue. There is a positive colour change attraction of ambient moisture to the test paper by the from the Dove Nourished Beauty Stick cosmetic compo- presence of the humectant in the cosmetic composition.

4 7 EP 3 156 793 A1 8

[0044] Commercially-available PEG-8 liquid, that is Ether, Steareth - 2, Steareth - 21, Parfum, Octyldo- typically used in the preparation of cosmetic composi- decanol, Persea Gratissima Oil, Glycerin, Panthe- tions, such as the Dove Advanced Care Stick range, con- nol,Trisodium EDTA,Geranil, Limonene, Citronellol, tains a small amount of moisture. The water content of Butylphenyl Methylpropional, Alpha-Isomethyl the PEG-8 liquid used is no more than 1%. A further test 5 lonone, Linalool was performed using the PEG-8 liquid alongside the commercially-available surfactant Tween-20. The com- [0048] Another aspect of the invention is a method of mercially-available Tween-20 liquid contains a maximum demonstrating the potential for an antiperspirancy effect moisturevalue of 3% water.The test wasperformed com- from a cosmetic composition. One commonly used form prising addition a drop of each of the liquids to a test10 ofantiperspirant agent is aluminiumcompounds, and alu- substrate and monitoring the results. Two pieces of mois- minium salts, such as aluminium chlorohydrate, alumin- ture paper were placed on a bench top surface. To one ium zirconium chlorohydrates and aluminium zirconium piece of paper, one drop of PEG-8 was added. To the chlorohydrate glycine complexes. A test for the presence other piece of paper, one drop of Tween-20 was added. of aluminium compounds could indicate the potential for The products were then left to react with the paper. 15 an antiperspirancy effect from a cosmetic composition. [0045] As shown in FIGURE 8, PEG-8 reacted strongly Also, more than one test can be performed at the same with the "Wator paper", whereas Tween-20 failed to pro- time. duce a positive test result. This seems to indicate that [0049] FIGURE 10 shows the results of tests for dem- the presence of PEG-8 itself was producing the positive onstrating the potential for a moisturisation and antiper- result, rather than the moisture present in either of the 20 spirancy effect from four different cosmetic compositions. two compositions. The components of the four different cosmetic composi- [0046] Another humectant used in commercial cos- tions tested are set out below: metic compositions is glycerol. The method of the inven- tion relates to a method of demonstrating the potential • Dove Purely Pampering Moisturising Lotion for a moisturisation effect from a cosmetic compositions, 25 Aqua, Glycerin, Stearic Acid, Caprylic/Capric Trig- using a fibrous substrate comprising a testing substance lyceride, Dimethicone, Glycol Stearate, PEG-100 for a humectant. This humectant may be glycerol. The Stearate, Cyclopentasiloxane, Petrolatum, Butyros- presence of glycerol can be detected, for example, by permum Parkii Butter, Tocopheryl Acetate, Potassi- the use of a glycerol assay kit obtainable from the Sigma um Lactate, Sodium PCA, Helianthus Annuus Hybrid Aldrich company. The commercially-available kit pro- 30 Oil (Hybrid), Isomerized Linoleic Acid (Isomerized), vides a means to determine glycerol concentrations by Urea, Collagen Amino Acids, Lactic Acid, Glyceryl a coupled enzyme assay comprising glycerol kinase and Stearate, Stearamide AMP, Triethanolamine, Cetyl glycerol phosphate oxidase, producing a colorimetric re- Alcohol, Acrylates/C10-30 Alkyl Acrylate Crosspol- sult. FIGURE 9 shows the results of an assay involving ymer, Carbomer, Disodium EDTA, parfum, Phenox- the detection of the humectant glycerol in a cosmetic35 yethanol, Methylparaben, Propylparaben, Cou- composition. marin, Hydroxyisohexyl 3-cyclohexene Carboxalde- [0047] The commercially-available glycerol assay test hyde, Titanium Dioxide substance was used at dilution factors 0.5 and 0.3, where the detector substance was diluted using de-mineralised • Dove Original RO water. The results in FIGURE 9 show that the Dove com- 40 Aqua, AluminiumChlorohydrate, Helianthus Annuus position produces a positive result for indicating the po- Seed Oil (Seed), Glycerin, Steareth-2, parfum, tential for a moisturisation effect from a cosmetic com- Steareth-20, Disodium EDTA, Tocopheryl Acetate, position at dilution factors of 0.5 and 0.3. The Nivea prod- Alpha-isomethyl lonone, Benzyl Benzoate, Benzyl uct does not produce such a visible colour change at Salicylate, Butylphenyl Methylpropional, Citronellol, either the 0.3 or the 0.5 dilution factors. The composition 45 Geraniol, Hexyl Cinnamal, Hydroxycitronellal, Lina- of the two materials used in the test are set out below. lool

• Dove INCI • Garnier Mineral InvisiDry RO Aqua, Aluminium Chlorohydrate, Helianthus Annuus Aqua, Aluminium Chlorohydrate, Cetearyl Alcohol, Seed Oil (Seed), Glycerin, Steareth-2, Parfum,50 Ceteareth-33, parfum (fragrance), Perlite, Phenox- Steareth-20, Disodium EDTA, Tocopheryl Acetate, yethanol, Dimethicone, L-limonene, Benzyl Sali- Alpha-isomethyl lonone, Benzyl Benzoate, Benzyl cylate, Benzyl Alcohol, Linalool, Geraniol, Citronel- Salicylate, Butylphenyl Methylpropional, Citronellol, lol, Methylisothiazolinone, Hexyl Cinnamal, F.I.L. Geraniol, Hexyl Cinnamal, Hydroxycitronellal, Lina- C38465/1 lool 55 • Nivea Powder Touch RO • Nivea INCI Aqua, Aluminium Chlorohydrate, PPG-15 Stearyl Aqua, Aluminium Chlorohydrate, PPG-15 Stearyl Ether, Steareth-2, Steareth-21, parfum, Kaolin, Per-

5 9 EP 3 156 793 A1 10

sea Gratissima Oil, Trisodium EDTA, L-limonene, Li- 6. A method according to any of the preceding claims nalool, Alpha-isomethyl lonone, Geraniol, Benzyl Al- wherein the cosmetic composition is in the form of a cohol, Coumarin stick, aerosol, roll-on or cream.

[0050] The four different cosmetic compositions were 5 7. A method according to any of the preceding claims, assessed using a method of demonstrating the potential wherein the cosmetic composition is in the form of a for moisturisation and an antiperspirancy effect. The first stick composition tested (Dove Purely Pampering) contains a humectant (glycerol) but no antiperspirant aluminium 8. A method according to any of the preceding claims, salts. As shown in FIGURE 10, this product therefore 10 wherein the detector substance is for an aluminium- shows a positive change in relation to "care", in other containing antiperspirant salt. words moisturisation but a negative result for an antiper- spirancy effect. 9. A method according to claim 8, wherein the detector [0051] The Garnier Mineral product shows a positive substance comprises Chromeazurol S or xylenol or- result for "protection", demonstrating the presence of alu- 15 ange. minium salts for an antiperspirant effect. However, there is a negative result in terms of "care" or moisturisation 10. A method according to any of the preceding claims, for this product. The same is true of the Nivea product wherein the detector substance is for a humectant. which again demonstrates an antiperspirant effect, but no moisturisation. 20 11. A methodaccording toclaim 10, whereinthe detector [0052] The last product (Dove Original) has a positive substance is for glycerol. result for "care", demonstrating the presence of the hu- mectant glycerol. The Dove Original product also dem- 12. A methodaccording toclaim 11, whereinthe detector onstrates the potential for an antiperspirant effect, by the substance comprises enzymes. positive colour change for the presence of antiperspirant 25 aluminium salts. The aluminium test results are due to 13. A methodaccording toclaim 10, whereinthe detector the presence of the Chromeazurol S within the test sub- substance is for polypropylene glycol or polyethyl- strate. ene glycol.

30 14. A methodaccording toclaim 13, whereinthe detector Claims substance comprises an inorganic salt and a pH in- dicator dye. 1. A method of demonstrating the potential for a mois- turisation and/or antiperspirancy effect from a cos- metic composition, said method comprising contact- 35 ing the composition with a detector substance for aluminium-containing antiperspirant salt and/or moisturiser, said detector substance generating a visible colour change to directly indicate the pres- ence of aluminium-containing antiperspirant salt40 and/or moisturiser, wherein such method is carried out at relative humidity of less than 80%.

2. A method according to claim 1 wherein the moistur- iser is a humectant. 45

3. A method according to claim 1 or 2 wherein the step of contacting the composition with the detector sub- stance comprises the application of the composition to a fibrous substrate comprising the detector sub- 50 stance.

4. A method according to claim 3, wherein in the fibrous substrate is paper. 55 5. A method according to any preceding of the preced- ing claims, wherein the cosmetic composition is an antiperspirant composition.

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