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ID Name Description Score P-Value Table S1. Escherichia coli gallium resistant and sensitive gene hits determined using a chemical genetic screen. Each individual score represents the mean of 9-12 trials. ID Name Description Score P-value Bifunctional aspartokinase/homoserine JW0001 thrA 0.0472 0.4974 dehydrogenase 1 JW0002 thrB Homoserine kinase 0.4535 0.0103 JW0003 thrC L-threonine synthase 0.0791 0.0185 JW0004 yaaX DUF2502 family putative periplasmic protein 0.0959 0.0095 Peroxide resistance protein, lowers intracellular JW0005 yaaA 0.0345 0.4262 iron JW0006 yaaJ Putative transporter 0.0623 0.1141 Molybdochelatase incorporating molybdenum JW0008 mog 0.0269 0.1935 into molybdopterin JW0009 yaaH Succinate-acetate transporter 0.0876 0.0017 JW0010 yaaW UPF0174 family protein 0.0694 0.2053 JW0012 yaaI UPF0412 family protein -0.0435 0.1445 JW0018 nhaA Sodium-proton antiporter -0.0091 0.7726 JW0019 nhaR Transcriptional activator of nhaa -0.0725 0.0417 JW0022 rpsT 30S ribosomal subunit protein S20 0.0020 0.9553 JW0024 ileS Isoleucyl-trna synthetase -0.1268 0.1086 FKBP-type peptidyl-prolyl cis-trans isomerase JW0026 fkpB 0.0334 0.5347 (rotamase) JW0028 rihC Ribonucleoside hydrolase 3 0.0069 0.9094 Carbamoyl phosphate synthetase small subunit, JW0030 carA 0.0893 0.1038 glutamine amidotransferase JW0031 carB Carbamoyl-phosphate synthase large subunit 0.0651 0.1219 JW0033 caiF Cai operon transcriptional activator 0.1140 0.0898 JW0035 caiD Carnitinyl-coa dehydratase 0.0250 0.5492 JW0037 caiB Crotonobetainyl coa:carnitine coa transferase 0.0036 0.9101 Crotonobetaine reductase subunit II, FAD- JW0038 caiA -0.0047 0.9263 binding JW0039 caiT Putative transporter 0.4032 0.0002 Anaerobic carnitine reduction putative electron JW0040 fixA 0.0580 0.1061 transfer flavoprotein subunit Putative electron transfer flavoprotein, JW0041 fixB NAD/FAD-binding domain and ETFP adenine -0.0146 0.6336 nucleotide-binding domain-like protein JW0042 fixC Putative oxidoreductase -0.0065 0.8901 JW0043 fixX Putative 4Fe-4S ferredoxin-type protein 0.0114 0.6153 Putative MFS sugar transporter; membrane JW0044 yaaU 0.2370 0.0040 protein Potassium-efflux system ancillary protein for kefc, JW0045 kefF glutathione-regulated; quinone oxidoreductase, 0.0365 0.4077 FMN-dependent JW0046 kefC Potassium:proton antiporter 0.0821 0.1587 JW0048 apaH Diadenosine tetraphosphatase 0.0322 0.4279 JW0049 apaG Protein associated with Co2+ and Mg2+ efflux 0.0018 0.9319 16S rrna m(6)A1518, m(6)A1519 JW0050 ksgA 0.0183 0.4308 dimethyltransferase, SAM-dependent 4-hydroxy-L-threonine phosphate JW0051 pdxA -0.0399 0.2976 dehydrogenase, NAD-dependent JW0052 surA Peptidyl-prolyl cis-trans isomerase (ppiase) 0.0615 0.0760 Membrane-anchored dnak co-chaperone, DNA- JW0054 djlA -0.0886 0.0213 binding protein JW0055 yabP Pseudogene, pentapeptide repeats-containing -0.0110 0.7437 Dual specificity 23S rrna pseudouridine(746), trna JW0057 rluA -0.0337 0.3005 pseudouridine(32) synthase, SAM-dependent RNA polymerase remodeling/recycling factor JW0058 hepA atpase; RNA polymerase-associated, ATP- -0.0350 0.0106 dependent RNA translocase JW0059 polB DNA polymerase II -0.1108 0.0259 Ara regulon transcriptional activator; JW0063 araC -0.0071 0.6309 autorepressor Thiamine/thiamine pyrophosphate ABC JW0065 thiQ -0.0016 0.9684 transporter atpase Thiamine/thiamine pyrophosphate ABC JW0066 thiP -0.0074 0.7744 transporter permease Thiamine/thiamine pyrophosphate/thiamine JW0067 tbpA monophosphate ABC transporter periplasmic -0.0807 0.0846 binding protein Transcriptional DNA-binding transcriptional JW0068 sgrR 0.0142 0.5347 activator of sgrs srna JW0069 setA Broad specificity sugar efflux system 0.1591 0.0062 JW0070 leuD 3-isopropylmalate dehydratase small subunit 0.1276 0.0071 JW0071 leuC 3-isopropylmalate dehydratase large subunit 0.2046 0.0029 JW0073 leuA 2-isopropylmalate synthase 0.3018 0.0007 JW0074 leuL Leu operon leader peptide 0.1839 0.0154 JW0075 leuO Global transcription factor -0.0954 0.0058 JW0076 ilvI Acetolactate synthase 3 large subunit -0.0369 0.3710 Acetolactate synthase 3, small subunit, valine- JW0077 ilvH -0.0466 0.2660 sensitive JW0079 mraZ Rsmh methytransferase inhibitor 0.0949 0.0169 16S rrna m(4)C1402 methyltransferase, SAM- JW0080 mraW -0.0108 0.7186 dependent JW0090 ddlB D-alanine:D-alanine ligase 0.0217 0.6523 Dgtp-preferring nucleoside triphosphate JW0097 mutT 0.0637 0.0378 pyrophosphohydrolase JW0099 yacF Ftsz stabilizer 0.0369 0.3961 JW0100 coaE Dephospho-coa kinase -0.0713 0.2217 JW0101 guaC GMP reductase -0.0906 0.0906 Assembly protein in type IV pilin biogenesis, JW0102 hofC 0.0423 0.2413 transmembrane protein T2SE secretion family protein; P-loop atpase JW0103 hofB 0.1302 0.2282 superfamily protein JW0104 ppdD Putative prepilin peptidase-dependent pilin 0.0307 0.2973 JW0105 nadC Quinolinate phosphoribosyltransferase 0.0531 0.2658 1,6-anhydro-N-acetylmuramyl-L-alanine JW0106 ampD -0.0325 0.2875 amidase, Zn-dependent; murein amidase Ampicillin resistance inner membrane protein; JW0107 ampE putative signaling protein in beta-lactamase 0.0507 0.1490 regulation JW0108 aroP Aromatic amino acid transporter -0.0040 0.9494 Pyruvate dehydrogenase complex repressor; JW0109 pdhR 0.3527 0.0006 autorepressor Pyruvate dehydrogenase, decarboxylase JW0110 aceE 0.0231 0.6135 component E1, thiamine triphosphate-binding Pyruvate dehydrogenase, JW0111 aceF -0.0074 0.9198 dihydrolipoyltransacetylase component E2 Dihydrolipoyl dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases JW0112 lpd 0.0169 0.6226 complexes; glycine cleavage system L protein; dihydrolipoamide dehydrogenase Aconitate hydratase 2; aconitase B; 2-methyl-cis- JW0114 acnB 0.0773 0.0493 aconitate hydratase JW0115 yacL UPF0231 family protein -0.0274 0.5653 JW0116 speD S-adenosylmethionine decarboxylase 0.0504 0.1356 Spermidine synthase (putrescine JW0117 speE -0.0934 0.0296 aminopropyltransferase) JW0118 yacC Puls_outs family protein 0.0396 0.0607 JW0119 cueO Multicopper oxidase (laccase) -0.0318 0.2142 JW0120 gcd Glucose dehydrogenase -0.0276 0.4399 JW0123 yadG Putative ABC transporter atpase -0.0423 0.1017 JW0124 yadH Putative ABC transporter permease -0.0854 0.0743 JW0125 yadI Putative PTS Enzyme IIA 0.0623 0.1383 JW0126 yadE Putative polysaccharide deacetylase lipoprotein 0.0522 0.0587 JW0127 panD Aspartate 1-decarboxylase -0.0540 0.3328 JW0129 panC Pantothenate synthetase 0.0956 0.0027 3-methyl-2-oxobutanoate JW0130 panB -0.0001 0.9988 hydroxymethyltransferase JW0131 yadC Putative fimbrial-like adhesin protein -0.0385 0.2861 JW0132 yadK Putative fimbrial-like adhesin protein 0.0591 0.0284 JW0133 yadL Putative fimbrial-like adhesin protein -0.0544 0.3251 JW0134 yadM Putative fimbrial-like adhesin protein -0.0475 0.1698 JW0135 htrE Putative outer membrane usher protein 0.0886 0.0250 JW0136 ecpD Putative periplasmic pilin chaperone 0.0361 0.0565 JW0137 yadN Putative fimbrial-like adhesin protein 0.0044 0.9023 Transcriptional regulator of rrna transcription; JW0141 dksA 0.0751 0.1690 dnak suppressor protein JW0142 sfsA Sugar fermentation stimulation protein A 0.0127 0.7298 JW0144 hrpB Putative ATP-dependent helicase -0.0254 0.2078 JW0145 mrcB Fused glycosyl transferase and transpeptidase 0.2493 0.0002 Iron(3+)-hydroxamate import ABC transporter JW0147 fhuC 0.0110 0.7885 atpase Iron(3+)-hydroxamate import ABC transporter JW0148 fhuD -0.0405 0.6255 periplasmic binding protein Iron(3+)-hydroxamate import ABC transporter JW0149 fhuB 0.0109 0.8317 permease JW0153 yadS UPF0126 family inner membrane protein 0.0828 0.0008 Vitamin B12 ABC transporter periplasmic binding JW0154 btuF 0.0151 0.5763 protein 5'-methylthioadenosine/S-adenosylhomocysteine JW0155 pfs -0.3293 0.0001 nucleosidase Deoxyguanosine triphosphate JW0156 dgt 0.0022 0.9574 triphosphohydrolase Serine endoprotease (protease Do), membrane- JW0157 degP 0.0039 0.9025 associated JW0159 yaeH UPF0325 family protein 0.0036 0.9317 JW0162 glnD Uridylyltransferase -0.0336 0.2002 JW0173 hlpA Periplasmic chaperone 0.0900 0.0279 Ribonuclease HII, degrades RNA of DNA-RNA JW0178 rnhB 0.0321 0.2498 hybrids JW0181 ldcC Lysine decarboxylase 2, constitutive -0.0490 0.2259 JW0182 yaeR Putative lyase -0.0432 0.3720 JW0185 yaeP UPF0253 family protein 0.0589 0.0006 JW0186 yaeQ PDDEXK superfamily protein 0.0076 0.8627 Alternative stalled-ribosome rescue factor B; JW0187 yaeJ 0.0106 0.8532 peptidyl-trna hydrolase, ribosome-attached Lipoprotein involved with copper homeostasis JW0188 nlpE -0.0371 0.3837 and adhesion Trna-Thr(GGU) m(6)t(6)A37 methyltransferase, JW0191 yaeB -0.0365 0.3118 SAM-dependent JW0192 rcsF Putative outer membrane protein 0.0082 0.7331 JW0193 metQ DL-methionine transporter subunit 0.0465 0.1112 JW0194 metI DL-methionine transporter subunit -0.0544 0.2145 JW0195 metN DL-methionine transporter subunit 0.0282 0.4134 JW0196 gmhB D,D-heptose 1,7-bisphosphate phosphatase -0.0387 0.2674 JW0197 dkgB 2,5-diketo-D-gluconate reductase B -0.0265 0.4403 JW0198 yafC Lysr family putative transcriptional regulator -0.0307 0.5736 Putative S-adenosyl-L-methionine-dependent JW0200 yafE -0.0890 0.1394 methyltransferase JW0202 gloB Hydroxyacylglutathione hydrolase -0.2974 0.0001 Putative S-adenosyl-L-methionine-dependent JW0203 yafS 0.0639 0.0796 methyltransferase Ribonuclease HI, degrades RNA of DNA-RNA JW0204 rnhA -0.0779 0.0007 hybrids JW0205 dnaQ DNA polymerase III epsilon subunit 0.0716 0.1749 JW0206 yafT Lipoprotein -0.0496 0.3501 JW0207 yafU Pseudogene 0.2145 0.0000 JW0210 ivy Inhibitor of c-type lysozyme, periplasmic 0.0009 0.0055 JW0212 lpcA D-sedoheptulose 7-phosphate isomerase -0.0396 0.3807 Type 2
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