Stable Isotopes and Gut Contents Indicate Differential Resource Use by Coexisting Asp (Leuciscus Aspius) and Pikeperch (Sander Lucioperca)
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Received: 21 December 2017 | Revised: 11 April 2018 | Accepted: 13 April 2018 DOI: 10.1111/eff.12414 ORIGINAL ARTICLE Stable isotopes and gut contents indicate differential resource use by coexisting asp (Leuciscus aspius) and pikeperch (Sander lucioperca) Mojmír Vašek1 | Antti P. Eloranta2 | Ivana Vejříková1 | Petr Blabolil1 | Milan Říha1 | Tomáš Jůza1 | Marek Šmejkal1 | Josef Matěna1 | Jan Kubečka1 | Jiří Peterka1 1Institute of Hydrobiology, Biology Centre of the Czech Academy of Sciences, České Abstract Budějovice, Czech Republic Differential use of habitat and prey resources is an important mechanism that may 2 Norwegian Institute for Nature Research, allow coexistence of sympatric species. Unlike interactions between smaller cyprinid Trondheim, Norway and percid fishes, the resource use by coexisting predatory asp (Leuciscus aspius) and Correspondence pikeperch (Sander lucioperca) is relatively unknown. Here, gut content and stable iso- Mojmír Vašek, Institute of Hydrobiology, Biology Centre of the Czech Academy of tope analyses were used to study ontogenetic dietary shifts and interspecific trophic Sciences, České Budějovice, Czech Republic. niche overlap between asp and pikeperch coexisting in two reservoirs. The hypoth- Email: [email protected] esis that both species show an ontogenetic dietary shift from small invertebrates to Funding information large fish prey, but at the same time use different prey resources to reduce potential Norwegian Financial Mechanism 2009– 2014, Grant/Award Number: 7F14316; competitive interactions, was validated. The isotopic niches of the two predators Grantová agentura České republiky, Grant/ showed no, or only a moderate, degree of overlap (0%–65%). The ontogenetic Award Number: 15-01625S; Horizon 2020 Framework Programme, Grant/Award changes in the degree of interspecific isotopic niche overlap were different in the Number: 677039; Norwegian Institute for two reservoirs, suggesting that trophic segregation can be dynamic and variable Nature Research among systems. Gut contents revealed that small (<100 mm standard length) asp consumed mostly terrestrial invertebrates and emerged aquatic insects, whereas small pikeperch foraged on zooplankton, larval and pupal stages of aquatic insects and fish. Larger individuals (>100 mm) of both species were predominantly piscivo- rous, with asp consuming more cyprinid prey and pikeperch more percid prey. Coexisting asp and pikeperch populations are able to utilise different prey resources, thereby reducing potential negative competitive interactions. KEYWORDS dietary ontogeny, foraging strategy, interspecific competition, piscivory, stable isotopes 1 | INTRODUCTION time of activity, the habitat used or the type of prey eaten (Schoener, 1986). A comprehensive review of resource use in fish communities Differential resource use is perceived as an important mechanism by Ross (1986) suggested that niche segregation among coexisting allowing the coexistence of species within ecological communi- species is mainly driven by partitioning of available food resources ties (Chase & Leibold, 2003; Chesson, 2000; Schoener, 1986). This rather than habitat or time segregation. Species coexistence can, view is based on the competitive exclusion principle (Hardin, 1960), however, be influenced also by other mechanisms. For example, sto- which states that species cannot stably coexist unless the utilisa- chastic events (e.g. unpredictable environmental fluctuations) that tion of limiting resources is well differentiated. The segregation of affect demographic attributes of species may result in their coexis- coexisting species can occur along various dimensions such as the tence (Grossman, Ratajczak, Crawford, & Freeman, 1998; Sale, 1978; Ecol Freshw Fish. 2018;1–12. wileyonlinelibrary.com/journal/eff © 2018 John Wiley & Sons A/S. | 1 Published by John Wiley & Sons Ltd 2 | VAŠEK ET AL. Warner & Chesson, 1985). Therefore, one approach to improve our the summer season. In contrast, Římov Reservoir (hereafter Římov; understanding of the ecological mechanisms that determine the co- 48°51′00″N, 14°29′28″E), situated on the Malše River, is a deep existence of species is to examine resource overlap among potential canyon- type lake (Table 1) that is strongly thermally stratified during competitors. Most studies of dietary segregation between coex- the summer season. Both reservoirs have similar water clarity and a isting fish species have focused on adult life stages (e.g. Hodgson, moderately eutrophic trophic status (Table 1). Schindler, & Kitchell, 1997; Schulze, Dörner, Baade, & Hölker, 2012; Due to seasonal water level fluctuations, the littoral zone vegeta- Walker, Kluender, Inebnit, & Adams, 2013; Zaia Alves et al., 2017). tion is poorly developed and submerged macrophytes are practically Ontogenetic variations in the resource use among potential com- missing in both reservoirs. The adult fish community compositions petitors have been examined less frequently (Amundsen et al., are similar in Lipno and Římov, with a dominance of cyprinid species 2003; Davis, Blanchette, Pusey, Jardine, & Pearson, 2012; Werner & (mostly roach Rutilus rutilus, bleak Alburnus alburnus, bream Abramis Gilliam, 1984) although this knowledge is important to fully under- brama and white bream Blicca bjoerkna) accompanied by perch Perca stand the structure and functioning of fish communities. fluviatilis and ruffe Gymnocephalus cernua (Čech et al., 2009; Vašek Asp (Cyprinidae, Leuciscus aspius) and pikeperch (Percidae, Sander et al., 2016). Asp and pikeperch naturally reproduce in both res- lucioperca) are important piscivorous fishes in freshwater communi- ervoirs (Blabolil et al., 2016; Jůza et al., 2013). In Římov, however, ties of western Eurasia where they naturally coexist in large rivers, populations of the two predators are also regularly supported by lakes and reservoirs (Kottelat & Freyhof, 2007; Vašek et al., 2013). stocking with pond- reared fingerlings in autumn (Vašek et al., 2013). Adults reach similar sizes (usually up to 1,000 mm in total length) and prey on small fish (Baruš & Oliva, 1995; Mittelbach & Persson, 1998). 2.2 | Sample collection Juveniles of pikeperch forage on aquatic invertebrates, whereas juveniles of asp may also feed on terrestrial insects fallen on the Fish sampling and treatment were conducted in compliance with water surface (Baruš & Oliva, 1995). The feeding ecology of pike- guidelines from the Experimental Animal Welfare Commission under perch has been explored extensively and thus it is well known that the Ministry of Agriculture of the Czech Republic. Asp, pikeperch this species usually shifts to piscivory in the first summer of its life and their fish prey were sampled from Lipno in August/September (Buijse & Houthuijzen, 1992; van Densen, Ligtvoet, & Roozen, 1996; 2012 and 2013 and from Římov in August 2013 and 2014. Sampling Mittelbach & Persson, 1998). Less is known, however, about the size was carried out with multimesh survey gillnets set overnight in lit- and age at which asp become piscivorous. Moreover, only limited toral, profundal and pelagic zones at four to five different stations attempts have been made to quantitatively characterise the diets of within each reservoir (for details of the gillnet sampling, see Vašek coexisting asp and pikeperch populations (Specziár & Rezsu, 2009). et al., 2016). Additional samples of young- of- the- year (YOY) asp and In general, similar feeding habits (i.e. invertivory followed by pisciv- pikeperch, as well as prey fish, were collected from the littoral and ory) suggest that the two species may interact strongly. Sympatric pelagic zones of both reservoirs using a beach seine net and a trawl populations of asp and pikeperch thus provide a good opportunity respectively (for details of these sampling methods, see Jůza et al., to investigate whether and how the two predators differ in resource 2014). use throughout their lives. Each fish was measured for standard length (mm), and a sam- In this study, gut content (GCA) and stable isotope (SIA) analyses ple of dorsal muscle was dissected and stored at −20°C until pro- were used to explore ontogenetic dietary shifts and niche segrega- cessed for stable isotope analysis. The analysed prey fish included tion between asp and pikeperch co- occurring in two artificial lakes YOY perch, ruffe and roach, and 1- year- old bleak. The digestive (i.e. reservoirs). It was expected that both species undergo an onto- genetic dietary shift from invertebrates to fish prey, but this shift occurs later (i.e. at a larger body size) for asp due to its higher ten- TABLE 1 Basic environmental characteristics of the two reservoirs studied. Mean values for the growing season (May– dency to feed on invertebrates. It was also hypothesised that coex- September) are shown for Secchi depth, total phosphorus and isting asp and pikeperch use different prey resources, but the degree chlorophyll a of trophic segregation diminishes with increasing body size, that is when both species become piscivorous. Characteristic Lipno Římov Year of filling 1960 1978 2 | METHODS Surface altitude (m a.s.l.) 725 471 Surface area (km2) 48.7 2.1 2.1 | Study sites Mean depth (m) 6 16 Maximum depth (m) 22 43 The study was carried out in two reservoirs located in South Bohemia, Hydraulic retention time (days) 244 85 Czech Republic. Lipno Reservoir (hereafter Lipno; 48°37′58″N, Secchi depth (m) 1.9 2.6 14°14′13″E), situated on the upper Vltava River, is a relatively shal- Total phosphorus ( g/L) 25 27