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Fluka/Chemfile Vol 3 No 4E.Qxd Sigma-Aldrich Worldwide Locations Argentina Germany Korea South Africa SIGMA-ALDRICH DE ARGENTINA, S.A. SIGMA-ALDRICH CHEMIE GmbH SIGMA-ALDRICH KOREA SIGMA-ALDRICH Tel: 54 11 4556 1472 Free Tel: 0800/51 55 000 Tel: 031-329-9000 Fax: 54 11 4552 1698 SOUTH AFRICA (PTY) LTD. Free Fax: 0800/64 90 000 Fax: 031-329-9090 Vol. 3 No. 6 Tel: 27 11 979 1188 Australia Greece SIGMA-ALDRICH PTY., LIMITED Malaysia Fax: 27 11 979 1119 SIGMA-ALDRICH (O.M.) LTD Free Tel: 1-800 800 097 SIGMA-ALDRICH (M) SDN. BHD Free Fax: 1-800 800 096 Tel: 210 9948010 Tel: 603-56353321 Spain Tel: (612) 9841 0555 Fax: 210 9943831 Fax: 603-56354116 Enzymes in SIGMA-ALDRICH QUÍMICA S.A. Fax: (612) 9841 0500 Hungary Mexico Free Tel: 900-101376 Austria SIGMA-ALDRICH Kft SIGMA-ALDRICH QUÍMICA, S.A. de C.V. 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Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip. SIGMA and - are registered trademarks of Sigma-Aldrich Co. and its division ® FVQ Sigma-Aldrich Biotechnology LP. Riedel-de Haën : trademark under license from Riedel-de Haën GmbH. 2 3 CUSTOM MANUFACTURING AT FLUKA 1. NEW! GLYCOSYLTRANSFERASE KITS FROM FLUKA Biocatalysts and Biotransformations → Are you working on glycosylations and differentiation, development and viral infection and are synthesis of oligosaccharides and diagnostic in certain cancers.[14] Oligosaccharides and The recognition of biocatalysts as important manufacturing bed adsorption, two-phase separation and membrane glycoconjugates? glycoconjugates, which serve as competitive ligands, represent tools has increased within the chemical and pharmaceutical chromatography. With these technologies the reproducible → valuable tools in biological studies and potential drug targets in industries in recent years. Biocatalysts can simplify, or in some production of biocatalysts is achieved in highest yields. Do you like to complement your synthetic techniques using biocatalysts? infectious deseases, inflammation and cancer. Glycosylation of instances even enable, the production of complex chemicals In order to fully satisfy the customer's demands, the production proteins and other bioactive molecules may serve in site specific → Do you like to avoid laborious multistep and drug intermediates. They can add stereospecificity to the of the biocatalyst can be combined with its direct application in and controlled drug delivery, to increase solubility of process, eliminating the need for complicated separation and procedures? a biotransformation. We have experience in routinely hydrophobic molecules,[15,16] alter uptake and residency time in purification steps. → performing more than a hundred biocatalytic processes and are Are you interested in high stereo- and vivo [17,18] and decrease antigenicity.[19] regioselectivity? Within Sigma-Aldrich's array of organic and biochemical ready for large-scale production using reactors with total The growing recognition of the roles of carbohydrates in capabilities, fermentation has had a long tradition with volumes of up to 1600 L. fundamental biological processes and their potential as new locations in the USA, Israel, and Switzerland. At our Fluka The techniques we use on the application side vary widely from The unique Fluka Glycosyltransferase Kits therapeutics has accentuated the requirement for a general facility located in Buchs, Switzerland, a wide range of chemical simple batch biotransformations over membrane reactors to include sufficient amounts of enzymes, availability of larger amounts of varying carbohydrate knowledge is combined with biotechnological expertise. Fluka whole-cell biotransformations. nucleotide sugar donors, buffers and reagents, structures. both develops and produces biocatalysts on small and large necessary for successful glycosylations on scales, and can subsequently perform the synthesis of the preparative scale. The isolation of glycoconjugates from natural sources provides only minute quantities, limiting carbohydrate structure and target molecule via biotransformation in-house. As part of our commitment to the progress of biocatalysis in function studies to the characterisation of glycan chains from Fluka synthetic chemistry, Fluka has, in the past few years, developed isolated from glycoproteins.[10] Moreover, it is nearly impossible and produced new glycosyltransferases for preparative to obtain homogeneous glycoproteins from overexpression Biocatalysts and carbohydrate synthesis as part of our commitment to the systems.[20,21] progress of biocatalysis in synthetic chemistry. Several Biotransformations recombinant glycosyltransferases are now available from well- The presence of multiple functional groups and stereocenters in established fermentation processes and can be offered for carbohydrates makes them challenging targets for the organic synthetic applications. Employing metabolic pathway chemist. Decades of synthetic research have not yielded robust, engineering researchers at Kyowa Hakko Kogyo Inc. (Tokyo, automated protocols comparable to those developed for the Galactosyltransferase Kits Japan) recently developed a large scale production system for preparation of peptides and oligonucleotides. Major issues for many nucleoside mono-and diphosphate sugar donors.[41-44] This the economic, large-scale, chemical synthesis of carbohydrates [1,6,22-24] technological breakthrough can be expected to enable and glycoconjugates are: industrial scale economic synthesis of oligosaccharides and → Multiple hydroxyl functionalities, which exhibit similar glycoconjugates in the near future.[7] reactivities, must be suitably differentiated in order to obtain In order to support and stimulate scientific research in the desired glycosidic linkages with suitable
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