Building a Glycosylation Platform in E. Co/I Through Metabolic Engineering Frederik De Bruyn
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Enzymatic Encoding Methods for Efficient Synthesis Of
(19) TZZ__T (11) EP 1 957 644 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: C12N 15/10 (2006.01) C12Q 1/68 (2006.01) 01.12.2010 Bulletin 2010/48 C40B 40/06 (2006.01) C40B 50/06 (2006.01) (21) Application number: 06818144.5 (86) International application number: PCT/DK2006/000685 (22) Date of filing: 01.12.2006 (87) International publication number: WO 2007/062664 (07.06.2007 Gazette 2007/23) (54) ENZYMATIC ENCODING METHODS FOR EFFICIENT SYNTHESIS OF LARGE LIBRARIES ENZYMVERMITTELNDE KODIERUNGSMETHODEN FÜR EINE EFFIZIENTE SYNTHESE VON GROSSEN BIBLIOTHEKEN PROCEDES DE CODAGE ENZYMATIQUE DESTINES A LA SYNTHESE EFFICACE DE BIBLIOTHEQUES IMPORTANTES (84) Designated Contracting States: • GOLDBECH, Anne AT BE BG CH CY CZ DE DK EE ES FI FR GB GR DK-2200 Copenhagen N (DK) HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI • DE LEON, Daen SK TR DK-2300 Copenhagen S (DK) Designated Extension States: • KALDOR, Ditte Kievsmose AL BA HR MK RS DK-2880 Bagsvaerd (DK) • SLØK, Frank Abilgaard (30) Priority: 01.12.2005 DK 200501704 DK-3450 Allerød (DK) 02.12.2005 US 741490 P • HUSEMOEN, Birgitte Nystrup DK-2500 Valby (DK) (43) Date of publication of application: • DOLBERG, Johannes 20.08.2008 Bulletin 2008/34 DK-1674 Copenhagen V (DK) • JENSEN, Kim Birkebæk (73) Proprietor: Nuevolution A/S DK-2610 Rødovre (DK) 2100 Copenhagen 0 (DK) • PETERSEN, Lene DK-2100 Copenhagen Ø (DK) (72) Inventors: • NØRREGAARD-MADSEN, Mads • FRANCH, Thomas DK-3460 Birkerød (DK) DK-3070 Snekkersten (DK) • GODSKESEN, -
Bioactive Components and Health Effects of Pecan Nuts and Their By- Products: a Review
Journal of International Society for Food Bioactives Nutraceuticals and Functional Foods Review J. Food Bioact. 2018;1:56–92 Bioactive components and health effects of pecan nuts and their by- products: a review Emilio Alvarez-Parrillaa, Rafael Urrea-Lópezb and Laura A. de la Rosaa* aDepartment of Chemical Biological Sciences, Universidad Autónoma de Ciudad Juárez, AnilloEnvolvente del Pronaf y Estocolmo, s/n, Cd, 32310 Juárez, Chihuahua, Mexico bCIATEJ, UnidadNoreste, Autopista Monterrey-Aeropuerto km 10.Parque PIIT. Vía de Innovación 404. Apodaca, N.L. México *Corresponding author: Laura A. de la Rosa, Department of Chemical Biological Sciences, Universidad Autónoma de Ciudad Juárez, AnilloEnvolvente del Pronaf y Estocolmo, s/n, Cd, 32310 Juárez, Chihuahua, Mexico. Tel: (+52) 656-688-1800 ext 1563; E-mail: ldelaros@ uacj.mx DOI: 10.31665/JFB.2018.1127 Received: January 18, 2018; Revised received & accepted: January 21, 2018 Citation: Alvarez-Parrilla, E., Urrea-López, R., and de la Rosa, L.A. (2018). Bioactive components and health effects of pecan nuts and their by-products: a review. J. Food Bioact. 1: 56–92. Abstract Pecan is a North American native tree that produces a stone fruit or kernel, commonly known as pecan nut,which is highly valuable worldwide due to its sensory quality, and health promoting properties derived from the pres- ence of mono- and polyunsaturated fatty acids, tocopherols and monomeric and polymeric polyphenolic com- pounds. The increase in the demand for pecan nut leads to an increase in by-products such as leaves, cake and principally nutshell, which have high contents of bioactive components, making them interesting raw materials to produce nutraceuticals with health benefits. -
Chondroprotective Agents
Europaisches Patentamt J European Patent Office © Publication number: 0 633 022 A2 Office europeen des brevets EUROPEAN PATENT APPLICATION © Application number: 94109872.5 © Int. CI.6: A61K 31/365, A61 K 31/70 @ Date of filing: 27.06.94 © Priority: 09.07.93 JP 194182/93 Saitama 350-02 (JP) Inventor: Niimura, Koichi @ Date of publication of application: Rune Warabi 1-718, 11.01.95 Bulletin 95/02 1-17-30, Chuo Warabi-shi, 0 Designated Contracting States: Saitama 335 (JP) CH DE FR GB IT LI SE Inventor: Umekawa, Kiyonori 5-4-309, Mihama © Applicant: KUREHA CHEMICAL INDUSTRY CO., Urayasu-shi, LTD. Chiba 279 (JP) 9-11, Horidome-cho, 1-chome Nihonbashi Chuo-ku © Representative: Minderop, Ralph H. Dr. rer.nat. Tokyo 103 (JP) et al Cohausz & Florack @ Inventor: Watanabe, Koju Patentanwalte 2-5-7, Tsurumai Bergiusstrasse 2 b Sakado-shi, D-30655 Hannover (DE) © Chondroprotective agents. © A chondroprotective agent comprising a flavonoid compound of the general formula (I): (I) CM < CM CM wherein R1 to R9 are, independently, a hydrogen atom, hydroxyl group, or methoxyl group and X is a single bond or a double bond, or a stereoisomer thereof, or a naturally occurring glycoside thereof is disclosed. The 00 00 above compound strongly inhibits proteoglycan depletion from the chondrocyte matrix and exhibits a function to (Q protect cartilage, and thus, is extremely effective for the treatment of arthropathy. Rank Xerox (UK) Business Services (3. 10/3.09/3.3.4) EP 0 633 022 A2 BACKGROUND OF THE INVENTION 1 . Field of the Invention 5 The present invention relates to an agent for protecting cartilage, i.e., a chondroprotective agent, more particularly, a chondroprotective agent containing a flavonoid compound or a stereoisomer thereof, or a naturally occurring glycoside thereof. -
Metabolites OH
H OH metabolites OH Article Genomic Survey, Transcriptome, and Metabolome Analysis of Apocynum venetum and Apocynum hendersonii to Reveal Major Flavonoid Biosynthesis Pathways Gang Gao , Ping Chen, Jikang Chen , Kunmei Chen, Xiaofei Wang, Aminu Shehu Abubakar, Ning Liu, Chunming Yu * and Aiguo Zhu * Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China; [email protected] (G.G.); [email protected] (P.C.); [email protected] (J.C.); [email protected] (K.C.); [email protected] (X.W.); [email protected] (A.S.A.); [email protected] (N.L.) * Correspondence: [email protected] (C.Y.); [email protected] (A.Z.); Tel.: +86-0731-8899-8507 (C.Y. & A.Z.) Received: 8 October 2019; Accepted: 2 December 2019; Published: 5 December 2019 Abstract: Apocynum plants, especially A. venetum and A. hendersonii, are rich in flavonoids. In the present study, a whole genome survey of the two species was initially carried out to optimize the flavonoid biosynthesis-correlated gene mining. Then, the metabolome and transcriptome analyses were combined to elucidate the flavonoid biosynthesis pathways. Both species have small genome sizes of 232.80 Mb (A. venetum) and 233.74 Mb (A. hendersonii) and showed similar metabolite profiles with flavonols being the main differentiated flavonoids between the two specie. Positive correlation of gene expression levels (flavonone-3 hydroxylase, anthocyanidin reductase, and flavonoid 3-O-glucosyltransferase) and total flavonoid content were observed. The contents of quercitrin, hyperoside, and total anthocyanin in A. venetum were found to be much higher than in A. hendersonii, and such was thought to be the reason for the morphological difference in color of A. -
Flavonol Glycosides from Clematis Cultivars and Taxa, and Their Contribution to Yellow and White Flower Colors
Bull. Natl. Mus. Nat. Sci., Ser. B, 34(3), pp. 127–134, September 22, 2008 Flavonol Glycosides from Clematis Cultivars and Taxa, and Their Contribution to Yellow and White Flower Colors Masanori Hashimoto1, Tsukasa Iwashina1,2,*, Junichi Kitajima3 and Sadamu Matsumoto2 1 Graduate School of Agriculture, Ibaraki University, Ami 300–0393, Japan 2 Department of Botany, National Museum of Nature and Science, Amakubo 4–1–1, Tsukuba 305–0005, Japan 3 Laboratory of Pharmacognosy, Showa Pharmaceutical University, Higashi-tamagawagakuen 3, Machida, Tokyo 194–8543, Japan * Corresponding author: E-mail: [email protected] Abstract The flower pigments in two yellow Clematis cultivars, “Gekkyuden” and “Manshu-ki”, and a yellow flower type of C. patens collected in Korea, were characterized. They were compared with those of three white Clematis florida varieties, var. florida, var. florepleno and var. sieboidiana. It was shown by UV-visible spectral survey of crude MeOH extract of their sepals that carotenoid pigment is apparently absent from yellow flowers. High performance liquid chromato- graphical and paper chromatographical survey of the flower pigments showed the presence of the flavonol glycosides. They were isolated and characterized by UV spectroscopy, acid hydrolysis, LC-MS, and direct HPLC and TLC comparisons with authentic samples. Quercetin 3-O-galacto- side (6) and 3-O-glucoside (7) were isolated from two yellow cultivars and a yellow flower type of C. patens as major components together with minor quercetin 3-O-rutinoside (8). On the other hand, kaempferol 3-O-rutinoside (9) and 3-O-glucoside (4) were detected in the white C. florida varieties as major compounds. -
Figure S1. Heat Map of R (Pearson's Correlation Coefficient)
Figure S1. Heat map of r (Pearson’s correlation coefficient) value among different samples including replicates. The color represented the r value. Figure S2. Distributions of accumulation profiles of lipids, nucleotides, and vitamins detected by widely-targeted UPLC-MC during four fruit developmental stages. The colors indicate the proportional content of each identified metabolites as determined by the average peak response area with R scale normalization. PS1, 2, 3, and 4 represents fruit samples collected at 27, 84, 125, 165 Days After Anthesis (DAA), respectively. Three independent replicates were performed for each stages. Figure S3. Differential metabolites of PS2 vs PS1 group in flavonoid biosynthesis pathway. Figure S4. Differential metabolites of PS2 vs PS1 group in phenylpropanoid biosynthesis pathway. Figure S5. Differential metabolites of PS3 vs PS2 group in flavonoid biosynthesis pathway. Figure S6. Differential metabolites of PS3 vs PS2 group in phenylpropanoid biosynthesis pathway. Figure S7. Differential metabolites of PS4 vs PS3 group in biosynthesis of phenylpropanoids pathway. Figure S8. Differential metabolites of PS2 vs PS1 group in flavonoid biosynthesis pathway and phenylpropanoid biosynthesis pathway combined with RNA-seq results. Table S1. A total of 462 detected metabolites in this study and their peak response areas along the developmental stages of apple fruit. mix0 mix0 mix0 Index Compounds Class PS1a PS1b PS1c PS2a PS2b PS2c PS3a PS3b PS3c PS4a PS4b PS4c ID 1 2 3 Alcohols and 5.25E 7.57E 5.27E 4.24E 5.20E -
Phenolic Constituentswith Promising Antioxidant and Hepatoprotective
id27907328 pdfMachine by Broadgun Software - a great PDF writer! - a great PDF creator! - http://www.pdfmachine.com http://www.broadgun.com December 2007 Volume 3 Issue 3 NNaattuurraall PPrrAoon dIdnduuian ccJotutrnssal Trade Science Inc. Full Paper NPAIJ, 3(3), 2007 [151-158] Phenolic constituents with promising antioxidant and hepatoprotective activities from the leaves extract of Carya illinoinensis Haidy A.Gad, Nahla A.Ayoub*, Mohamed M.Al-Azizi Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, (EGYPT) E-mail: [email protected] Received: 15th November, 2007 ; Accepted: 20th November, 2007 ABSTRACT KEYWORDS The aqueous ethanolic leaf extract of Carya illinoinensis Wangenh. K.Koch Carya illinoinensis; (Juglandaceae) showed a significant antioxidant and hepatoprotective Juglandaceae; activities in a dose of 100 mg/ kg body weight. Fifteen phenolic compounds Phenolic compounds; were isolated from the active extract among which ten were identified for Hepatoprotective activity. the first time from Carya illinoinensis . Their structures were elucidated to be gallic acid(1), methyl gallate(2), P-hydroxy benzoic acid(3), 2,3-digalloyl- 4 â 4 -D- C1-glucopyranoside(4), kaempferol-3-O- -D- C1-galactopyranoside, ’-O-galloyl)- 4 trifolin(8), querectin-3-O-(6' -D- C1-galactopyranoside(9), ’-O-galloyl)- 4 kaempferol-3-O-(6' -D- C1-galactopyranoside(10), ellagic acid(11), 3,3' dimethoxyellagic acid(12), epigallocatechin-3-O-gallate(13). Establishment of all structures were based on the conventional methods of analysis and confirmed by NMR spectral analysis. 2007 Trade Science Inc. - INDIA INTRODUCTION dition, caryatin(quercetin-3,5-dimethyl ether) , caryatin glucoside and rhamnoglucoside were also isolated from Family Juglandaceae includes the deciduous gen- the bark[4], while, quercetin glycoside, galactoside, rham- era, Juglans(walnuts) and Carya(hickories). -
Nanor:Noles C/Mg Protein
Lawrence Berkeley National Laboratory Recent Work Title INTERRELATIONSHIP OF GLYCOGEN METABOLISM AND LACTOSE SYNTHESIS IN MAMMARY EPITHELIAL CELLS OF MICE Permalink https://escholarship.org/uc/item/2r05952r Author Emerman, J.T. Publication Date 1979-10-01 eScholarship.org Powered by the California Digital Library University of California LBL-9894~. d. {/ Preprint ITlI Lawrence Berkeley Laboratory .-;:I UNIVERSITY OF CALIFORNIA CHEMICAL BIODYNAMICS DIVISION Submitted to the Journal of Biological Chemistry -- INTERRELATIONSHIP OF GLYCOGEN METABOLISM AND LACTOSE SYNTHESIS IN MAMMARY EPITHELIAL CELLS OF MICE Joanne .T. Emerman, Jack C. Bartley, and Mina J. Bissell October 1979 RECEIVED LAWRENCE TWO-W E,K LOAN COpy LIBRARY )lIND DOCUMENT.S SEeT!G This is a Library Circulatin9 Copy which may be borrowed for two weeks. For a personal retention" ~, copy, call Tech. Info. Diuision, "Ext. .. 6782 . - "'; . , f.·l~ " /(, Prepared for the U.S. Department of Energy under Contract W-7405-ENG-48 DISCLAIMER This document was prepared as an account of work sponsored by the United States Government. While this document is believed to contain correct information, neither the United States Government nor any agency thereof, nor the Regents of the University of California, nor any of their employees, makes any warranty, express or implied, or assumes any legal responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by its trade name, trademark, manufacturer, or otherwise, does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof, or the Regents of the University of California. -
Phytochemical Constituents and Pharmacological Activities of Plants from the Genus Adiantum : a Review
Pan et al Tropical Journal of Pharmaceutical Research October 2011; 10 (5): 681-692 © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved . Available online at http://www.tjpr.org http://dx.doi.org/10.4314/tjpr.v10i5.18 Review Article Phytochemical Constituents and Pharmacological Activities of Plants from the Genus Adiantum : A Review 1 1 1 1 1 2 C Pan , YG Chen *, XY Ma , JH Jiang , F He and Y Zhang 1Department of Chemistry, Yunnan Normal University, Kunming 650500, 2School of Pharmacy, Kunming Medical College, Kunming 650031, China. Abstract Adiantum is a genus of ca. 200 species in the family Adiantaceae, distributed extensively across the world from cool temperate zones to hot tropical regions. A lot of Adiantum species have been used in traditional Chinese medicine to cure human and animal diseases including relief of internal heat or fever, enhancement of urination, removal of urinary calculus, and sundry other curative claims. Chemical studies have shown the presence of various classes of compounds, the main ones being triterpenoids, flavonoids, phenyl propanoids, steroids, alicyclic acids, lipids and long-chain compounds. The extract of this genus as well as pure compounds isolated from it have been demonstrated to possess multiple pharmacological activities such as analgesic, antinociceptive, anti-implantation, and antimicrobial activities. In this review, we have addressed the phytochemistry and pharmacological activities of the Adiantum species in order to collate existing information on this plant as well as highlight its multi-activity properties as a medicinal agent. Keywords: Adiantum species, Adiantaceae, Phytochemical constituents, Pharmacological activities. -
Analysis of the Binding and Interaction Patterns of 100 Flavonoids with the Pneumococcal Virulent Protein Pneumolysin: an in Silico Virtual Screening Approach
Available online a t www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2016, 8 (16):40-51 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4 Analysis of the binding and interaction patterns of 100 flavonoids with the Pneumococcal virulent protein pneumolysin: An in silico virtual screening approach Udhaya Lavinya B., Manisha P., Sangeetha N., Premkumar N., Asha Devi S., Gunaseelan D. and Sabina E. P.* 1School of Biosciences and Technology, VIT University, Vellore - 632014, Tamilnadu, India 2Department of Computer Science, College of Computer Science & Information Systems, JAZAN University, JAZAN-82822-6694, Kingdom of Saudi Arabia. _____________________________________________________________________________________________ ABSTRACT Pneumococcal infection is one of the major causes of morbidity and mortality among children below 2 years of age in under-developed countries. Current study involves the screening and identification of potent inhibitors of the pneumococcal virulence factor pneumolysin. About 100 flavonoids were chosen from scientific literature and docked with pnuemolysin (PDB Id.: 4QQA) using Patch Dockprogram for molecular docking. The results obtained were analysed and the docked structures visualized using LigPlus software. It was found that flavonoids amurensin, diosmin, robinin, rutin, sophoroflavonoloside, spiraeoside and icariin had hydrogen bond interactions with the receptor protein pneumolysin (4QQA). Among others, robinin had the highest score (7710) revealing that it had the best geometrical fit to the receptor molecule forming 12 hydrogen bonds ranging from 0.8-3.3 Å. Keywords : Pneumococci, pneumolysin, flavonoids, antimicrobial, virtual screening _____________________________________________________________________________________________ INTRODUCTION Streptococcus pneumoniae is a gram positive pathogenic bacterium causing opportunistic infections that may be life-threating[1]. Pneumococcus is the causative agent of pneumonia and is the most common agent causing meningitis. -
(12) STANDARD PATENT (11) Application No. AU 2015215937 B2 (19) AUSTRALIAN PATENT OFFICE
(12) STANDARD PATENT (11) Application No. AU 2015215937 B2 (19) AUSTRALIAN PATENT OFFICE (54) Title Metabolically engineered organisms for the production of added value bio-products (51) International Patent Classification(s) C12N 15/52 (2006.01) C12P 19/26 (2006.01) C12P 19/18 (2006.01) C12P 19/30 (2006.01) (21) Application No: 2015215937 (22) Date of Filing: 2015.08.21 (43) Publication Date: 2015.09.10 (43) Publication Journal Date: 2015.09.10 (44) Accepted Journal Date: 2017.03.16 (62) Divisional of: 2011278315 (71) Applicant(s) Universiteit Gent (72) Inventor(s) MAERTENS, Jo;BEAUPREZ, Joeri;DE MEY, Marjan (74) Agent / Attorney Griffith Hack, GPO Box 3125, Brisbane, QLD, 4001, AU (56) Related Art Trinchera, M. et al., 'Dictyostelium cytosolic fucosyltransferase synthesizes H type 1 trisaccharide in vitro', FEBS Letters, 1996, Vol. 395, pages 68-72 GenBank accession no. AF279134, 6 May 2002 van der Wel, H. et al., 'A bifunctional diglycosyltransferase forms the Fuc#1,2Gal#1,3-disaccharide on Skpl in the cytoplasm of Dictyostelium', The Journal of Biological Chemistry, 2002, Vol. 277, No. 48, pages 46527-46534 WO 2010/070104 Al Abstract The present invention relates to genetically engineered organisms, especially microorganisms such as bacteria and yeasts, for the production of added value bio-products such as specialty saccharide, activated saccharide, nucleoside, glycoside, glycolipid or glycoprotein. More specifically, the present invention relates to host cells that are metabolically engineered so that they can produce said valuable specialty products in large quantities and at a high rate by bypassing classical technical problems that occur in biocatalytical or fermentative production processes. -
Epithelial Cells of Mice
Biochem. J. (1980) 192, 695-702 695 Printed in Great Britain Interrelationship of glycogen metabolism and lactose synthesis in mammary epithelial cells of mice Joanne T. EMERMAN*, Jack C. BARTLEYt and Mina J. BISSELL Laboratory ofChemical Biodynamics, Lawrence Berkeley Laboratory, University ofCalifornia, Berkeley, CA 94720, U.S.A.t (Received 9 May 1980/Accepted 21 July 1980) Glycogen metabolism in mammary epithelial cells was investigated (i) by studying the conversion of glucose into glycogen and other cellular products in these cells from virgin, pregnant and lactating mice and (ii) by assaying the enzymes directly involved with glycogen metabolism. We find that: (1) mammary epithelial cells synthesized glycogen at rates up to over 60% that of the whole gland; (2) the rate of this synthesis was modulated greatly during the reproductive cycle, reaching a peak in late pregnancy and decreasing rapidly at parturition, when abundant synthesis of lactose was initiated; (3) glycogen synthase and phosphorylase activities reflected this modulation in glycogen metabolism; (4) lactose synthesis reached a plateau during late pregnancy, even though lactose synthase is reported to increase in the mouse mammary gland at this time. We propose that glycogen synthesis restricts lactose synthesis during late pregnancy by competing successfully for the shared UDP-glucose pool. The physiological advantage of glycogen accumulation during late pregnancy is discussed. The significance of glycogen metabolism in possibly because the cultures were known to be mammary epithelial cells has not been considered contaminated with other types of cells. seriously. Putative glycogen granules have been In a preliminary study on the metabolic patterns identified in these cells in the dog (Sekri & Faulkin, of the mammary gland at different stages of the 1967), cow (Reid & Chandler, 1973) and human reproductive cycle, we found that glands from (Sterling & Chandler, 1977).