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Phenolic Constituentswith Promising Antioxidant and Hepatoprotective id27907328 pdfMachine by Broadgun Software - a great PDF writer! - a great PDF creator! - http://www.pdfmachine.com http://www.broadgun.com December 2007 Volume 3 Issue 3 NNaattuurraall PPrrAoon dIdnduuian ccJotutrnssal Trade Science Inc. Full Paper NPAIJ, 3(3), 2007 [151-158] Phenolic constituents with promising antioxidant and hepatoprotective activities from the leaves extract of Carya illinoinensis Haidy A.Gad, Nahla A.Ayoub*, Mohamed M.Al-Azizi Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, (EGYPT) E-mail: [email protected] Received: 15th November, 2007 ; Accepted: 20th November, 2007 ABSTRACT KEYWORDS The aqueous ethanolic leaf extract of Carya illinoinensis Wangenh. K.Koch Carya illinoinensis; (Juglandaceae) showed a significant antioxidant and hepatoprotective Juglandaceae; activities in a dose of 100 mg/ kg body weight. Fifteen phenolic compounds Phenolic compounds; were isolated from the active extract among which ten were identified for Hepatoprotective activity. the first time from Carya illinoinensis . Their structures were elucidated to be gallic acid(1), methyl gallate(2), P-hydroxy benzoic acid(3), 2,3-digalloyl- 4 â 4 -D- C1-glucopyranoside(4), kaempferol-3-O- -D- C1-galactopyranoside, ’-O-galloyl)- 4 trifolin(8), querectin-3-O-(6' -D- C1-galactopyranoside(9), ’-O-galloyl)- 4 kaempferol-3-O-(6' -D- C1-galactopyranoside(10), ellagic acid(11), 3,3' dimethoxyellagic acid(12), epigallocatechin-3-O-gallate(13). Establishment of all structures were based on the conventional methods of analysis and confirmed by NMR spectral analysis. 2007 Trade Science Inc. - INDIA INTRODUCTION dition, caryatin(quercetin-3,5-dimethyl ether) , caryatin glucoside and rhamnoglucoside were also isolated from Family Juglandaceae includes the deciduous gen- the bark[4], while, quercetin glycoside, galactoside, rham- era, Juglans(walnuts) and Carya(hickories). The spe- noside, arabinoside and kaempferol-3-monomethyl cies Carya illinoinensis Wangenh. K.Koch, known in ether were identified in the leaves. The present study English as Pecan, is a large, vase-shaped tree of a 25- describes the isolation and structure elucidation of phe- 45m tall and a trunk of 2.1m in diameter. The leaves are nolic metabolites from the aqueous alcohol extract of alternate, pinnately compound, 10 to 20 inches long, the leaves Carya illinoinensis(Juglandaceae) of prom- 11 to 17 leaflets that are 4 to 8 inches long[1]. Pecan ising hepatoprotective activity. trees produce edible nuts that have a high percentage of fat and are used extensively in candies and cook- EXPERIMENTAL ies[2]. It was used by the Comanche as a treatment for ringworm[3]. The Kiowa would consume a concentrate Plant materials [3] made from the bark of pecan for tuberculosis . Among Fresh leaves of Carya illinoinensis Wangenh. the previously identified phenolics of C. illinoinensis, K.Koch(Juglandaceae) were collected on May, 2005, azaleatin(quercetin-5-methyl ether) and azaleatin glu- from a mature tree at the yellow mountain area, Cairo, coside, arabinoside, rutinoside and rhamnoside . In ad- Egypt and identified by Professor Dr.Abdel Salam 152 Phenolic constituents with promisin. g antioxidant and hepatoprotective NPAIJ, 3(3) December 2007 Full Paper Mohamed AL-Nowiahai, Professor of Taxonomy, Fac- acid)] to form a stable yellow colour of 5-thio-2- ulty of Science, Ain Shams University. The fresh leaves nitrobenzoic acid, which can be measured at 412nm. were collected at(May-August). A voucher specimen In order to determine the glutathione level in the blood, has been deposited at the herbarium of the department precipitation of protein SH-groups was necessary be- ’s reagent. Blood sample(0.1 of pharmacognosy, faculty of pharmacy, Ain-Shams Uni- fore the addition of Ellman versity, Cairo, Egypt. ml) was haemolysed by the addition of 0.9ml bidistilled Plant extract water. To the haemolysate, 1.5ml of the precipitating solution was added, mixed and allowed to stand for The fresh leaves were air-dried in shade, reduced 5minutes. Centrifugation at 3000 rpm was carried out to fine powder. The powdered leaves(500g) were ex- for 15min. To 1ml of the resulting supernatant, 4ml of haustively extracted with water. The extract was filtered phosphate buffer solution was added followed by 0.5ml 0 ’s reagent. The optical density was measured and evaporated in vacuo at 45 C till dryness. The dried of Ellman material was extracted with ethanol(3 times, each with within 5 minutes at 412nm using the Shimadzu double 500ml ethanol for 8hrs, under reflux over hot water beam spectrophotometer(UV-150-02). The blank so- bath). The collected ethanol portions were evaporated lution for the samples was prepared with 4ml phos- in vacuum till dryness. Then the dry residual powder(total phate solution, 1ml dilute precipitating solution(3:2) and ’s reagent. To 1ml standard glutathione extract, 55g) were then subjected to phytochemical and 0.5ml Ellman biological investigations. solution, 4ml phosphate buffer solution and 0.5ml ’s reagent were added and the optical density Biological assays Ellman 1. Experimental animals was measured at 412nm against blank containing 1ml bidistilled water instead of the standard solution using Experimental animals used consisted of albino mice the following equation: of 25-30g body weight and adult male albino rats of absorbanceof sample 37.5 2.5 sprange dawely strain of 130-150g body weight. Doses GSH mg% 100 absorbanceof thestandard 1000 0.1 of the drugs used were calculated according to Paget ’s All statistical analyses were performed using the stu- and Barne [5] and were administered orally by gastric “t” test as described by Spedecor and Cochran dent [8]. tubes. 4. Hepatoprotective activity 2. Determination of the median lethal dose(LD50) Liver damage in rats was induced according to the The LD of the aqueous-ethanol extract of 50 method of Klassen and Plaa[9] by intraperitoneal injec- C.Illinoinensis was estimated according to the Karber tion of 5ml/Kg of 25% carbon tetrachloride(CCl ) in procedure[6]. Preliminary experiments were done to de- 4 liquid paraffin. Animals were randomly divided into three termine the minimal dose that kills all animals(LD ) 100 groups each of 10 rats: and the maximal dose that did not kill any animal. Sev- 1. First group: Represented control group, received a eral doses at equal logarithmic intervals were chosen in daily oral dose of 1ml saline for one month before between, each dose was injected in a group of 6 ani- and after liver damage. mals by subcutaneous injection. The mice were then 2. Second group: Liver damaged rats were pretreated observed for 24 hours and symptoms of toxicity and with a daily oral dose of 100mg/Kg body weight of mortality rates in each group were recorded and the the extract for one month. Administration of the LD was calculated. 50 extract was continued for one more month. 3. Antioxidant activity 3. Third group: Liver damaged rats were pretreated This method depends on measuring the blood glu- with a daily oral dose of 25mg/Kg body weight of tathione level and was determined according to the silymarin. Administration of the drug(silymarin) was method of Beutler et al.[7]. The method depends on the continued after liver damage for one more month. fact that both protein and non protein SH-groups react The whole blood was obtained from the retro-or- ’s reagent[5,5-dithiobis-(2-nitrobenzoic with Ellman bital venous plexus through the eye canthus of anaes- NaturaAln PInrdioand Jouurcnatl s NPAIJ, 3(3) December 2007 Nahla A.Ayoub et al. 153 Full Paper thetized rat after overnight fasting. Blood samples were Methyl 3,4,5-trihydroxybenzoate, Methyl gallate(2) was collected at zero time, one month and 72hrs after CCl obtained as an off-white amorphous powder(26mg); 4 injection and after one month intervals. Serum was iso- Rf values: 48(HOAc-6%) and 73(BAW); UV max : [10] [11] lated by centrifugation(Serum ALT, AST and ALP) . MeOH(279nm), MeOH/KOH(236, 278, 317), ’s 1 The data obtained were analyzed using the student AlCl3(298sh, nullified by adding HCl). H-NMR data: [9] 1 t test(Snedecor and Cochran 1991) . ppm 6.91(2H, s, H-2, H-6), 3.3(3H, s, -CH3). H- Isolation and structural elucidation NMR data was identical to that reported in literature [14-15]. The prepared extract(55g) was fractionated on a p-Hydroxy benzoic acid(3) was obtained as an off- sephadex LH-20 column,(100cm 5cm) eluted with dis- white amorphous powder(125mg); R values: 36 ë f tilled water, followed by mixtures of water/methanol of (HOAc-6%) and 84(BAW); UV : MeOH (253). äppm MAX decreasing polarities and finally by acetone. Fractions 1H-NMR data 7.8(d, J=9Hz, H-2, H-6), 6.8(d, äppm of 1-2L. were collected and monitored by 2D paper J=9Hz, H-3, H-5). 13C-NMR data : 121.6 (C- chromatography using solvent systems; BAW/6% ace- 1), 131.3(C-2 & C-6), 115.1(C-3 & C-5), 160.6 (C- tic acid. Similar fractions were pooled together to give 4), 167.7(C=O). 1H- and 13C-NMR data were identi- six main fractions(I-VI). 2D-PC, showed that fraction cal to those reported in literature[16]. I consisted mainly of carbohydrates, fraction II-V con- 4 2,3-digalloyl- -D- C1-glucopyranoside, nilocitin tain mainly phenolic constituents and fraction VI con- (4) was obtained as an off-white amorphous powder(26 tained condensed tannins. A combination of column mg); R values: 73(HOAc-6%) and 33(BAW); UV f MAX fractionation and PPC led to isolation of fifteen pure : MeOH(253). 1H-NMR data: ppm -glucose; 5.4(d, phenolics from fractions(II-V). Elution with H O gave 2 J=3.3Hz, H-1), 4.92(dd, J=8, 3.3Hz, H-2), 5.78(t, â rise to fraction I(25g, 4L).
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