2005-2016 [CURRICULUM VITAE]
Name: Khosravi Ayyoob Birth Date: 1980, march 21 Marital status: Married Affiliation: Golestan University of Medical Sciences, Faculty of Advanced Medical Technologies, department of Molecular Medicine E-mail address: [email protected] Telephone: Office: +98-17-32425995 Education: Degree Date Major University Ph.D 2011- 2016 molecular medicine Golestan University of Medical Sciences, Gorgan M.Sc 2002-2005 Cell and Molecular Biology Imam Hussein University, Tehran B.Sc 1998-2001 Biology Shahid Beheshti University, Tehran
Position 2006-2009 Instructor in Shiraz University of Medical Sciences 2010-2011 Instructor in Golestan University of Medical Sciences 2016- … Assistant professor in Golestan University of Medical Sciences Research interests Molecular Medicine (Cancer biology, Human Cancer Stem cell and Human genetic) Medical biotechnology (Cell Line Development & Engineering) Specialized techniques: 1-Molecular biology: - DNA and RNA extraction (from Humans, Bacteria and Viruses) - PCR (Nested PCR, RT-PCR, ARMS-PCR and Real time-PCR) -Horizontal (agarose) and vertical Electrophoresis (PAGE) - SDS-PAGE -SSCP (single strand conformational polymorphism) -Gel extraction and purification of PCR product
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- DNA cloning - RFLP (restriction fragment length polymorphism) - DNA typing by Short Tandem Repeats (STRs) -construction of DNA typing ladders 2- Molecular virology: - Quantification of HIV, HBV and HCV genes by Real-time PCR - PCR detection and genotyping of HIV, HBV and HCV viruses 3-Immunology: - ELISA (Enzyme-linked immunosorbent assay) - Western blot - Indirect Immunofluorescent Assay (IFA) - Immunocytochemistry (ICC) 4- Cell Culture: - Basic animal cell culture - Primary culture of Human Cancer Cells - Cancer stem cell biology Teaching experiences Course Location (University) Level Date cell and molecular biology Shiraz university of medical B.Sc of experimental 2008 sciences sciences Biosafety principles in Shiraz university of medical B.Sc of experimental 2008 laboratory practices sciences sciences cell and molecular biology Shiraz university of medical B.Sc of experimental 2009 sciences sciences Biosafety principles in Shiraz university of medical B.Sc of experimental 2009 laboratory practices sciences sciences cell and molecular biology Shiraz university of medical B.Sc of experimental 2010 sciences sciences Biosafety principles in Shiraz university of medical B.Sc of experimental 2010 laboratory practices sciences sciences Medical genetics Shiraz university of medical B.Sc of experimental 2010
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sciences sciences Molecular genetics methods Golestan University of MSc of medical genetic 2011 Medical Sciences cell and molecular biology Golestan University of B.Sc of experimental 2011 Medical Sciences sciences Molecular diagnosis of Golestan University of Ph.D of molecular 2011 Medical Sciences diseases medicine genetic engineering Golestan University of MSc of medical genetic 2011 Medical Sciences Molecular genetics methods Golestan University of MSc of medical genetic 2012 Medical Sciences cell and molecular biology Golestan University of B.Sc of experimental 2012 Medical Sciences sciences genetic engineering Golestan University of MSc of medical genetic 2013 Medical Sciences genetic engineering Golestan University of MSc of medical 2013 Medical Sciences biotechnology genetic engineering Golestan University of MSc of medical genetic 2013 Medical Sciences
Language Skills Persian and English Computer Knowledge Well in science-related softwares such as DNAsis, Oligo, GENE RUNNER, SPSS, PHYLIP, Endnote, SNPStats, ImageJ and... Research projects: 1- Title of the Project: characterization of human immunodeficiency virus type-1-infected plasma by near-infrared Spectroscopy Kind of Cooperation: as main co-worker Year: 2006-2007 2- Title of the Project: Analysis of the correlation between IL-18 promoter polymorphisms, IL-18 and IgE serum levels in asthmatic patients. Kind of Cooperation: as main co-worker Year: 2007-2009
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3- Title of the Project: Investigation of matrix metalloproteinase polymorphisms and relation to common cancers in Fars province. Kind of Cooperation: as main co-worker Year: 2008-2009 4- Title of the Project: Interleukin-1α (IL-1 α) and interleukin-1 receptor antagonist (IL- 1RN) gene polymorphisms in MS patients and healthy individuals Kind of Cooperation: as executive of project Year: 2011-2012 5- Title of the Project: Association of torsade de pointes with Allelic variants of long QT syndrome genes in Iranian population Kind of Cooperation: as executive of project Year: 2011-2012 6- Title of the Project: Computational prediction of the effects of coding single nucleotide polymprphisms in human endothelial nitric oxide synthase gene Kind of Cooperation: as executive of project Year: 2011-2012 7- Title of the Project: Association of polymorphisms in MCP-1 promoter variant –2518 and Interferon gama +874 T/A with suscep bility to pulmonary tuberculosis in Golestan province, Iran Kind of Cooperation: as executive of project Year: 2011-2012 8- Title of the Project: Characterization of transcriptional co-activator (TAZ) as a candidate cancer stem cell marker in esophageal Squamous Cell Carcinoma (ESCC) Kind of Cooperation: as executive of project Year: 2013-2015 9-Title of the Project: Inves ga on of Th1, Th17 and T reg cells frequency in esophageal cancer patients and their association with mir155, mir21, mir106a, mir29b and TGF-β plasma level Kind of Cooperation: as executive of project Year: 2016-2017
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Publications: 1- Mirza Khalil Bahmani, Ayyoob Khosravi, Fereidoun Mahboodi, Ramin Sarrami- Forooshani.Genotypic correlates of a virologic response to Lamivudine, Stavudine and Nevirapine in patients whom combination therapy had failed. Iranian journal of Infec ous Disease, 2008; 3(4):215-219. 2- Mirza Khalil Bahmani, Ayyoob Khosravi, Ramin Miri, Yukie Iwabu, Kazuyoshi Ikuta and Akikazu Sakudo. Spectroscopic characterization of human immunodeficiency virus type-1-infected plasma by principal component analysis and soft independent modeling of class analogy of visible and near-infrared spectra. Molecular Medicine REPORTS, 2009; 2: 805-809. 3- Mirza Khalil Bahmani, Ayyoob Khosravy, Adel Mobasser, Islam Ghezelsofla. Seroprevalence of Hepatitis B Virus (HBV) Infection and Vaccination Compliance among Health Care Workers (HCWs) in Fars Province, Iran. Iranian journal of Infectious Disease, 2010; 5(1):45-50. 4- Rahimi A. A, Bahmani M. Kh, Masoudnejad A, Sa’adati M, Khosravi A. Shafi’khani A, Mohammadzade J. Application of near-infrared spectroscopy and support vector machine in detection of HIV-1 infec on. Kowsar Medical Journal 2010; 15 (1): 23-29. 5- Ayyoob Khosravi, Mirzakhalil Bahmani, Islam Ghezel-Sofla. Co-Infection by Hepatitis C Virus in human immunodeficiency virus Infected Patients in southwest of Iran. Iranian journal of Infectious Disease. 2010; 5 (4):223-227. 6- Samaneh Hajihoseini, Mirza Khalil Bahmani, Ayyoob Khosravi. Eslam Ghezelsofla, Abbas Ghaderi. Prognostic significance of MMP2 and MMP9 functional promoter single nucleotide polymorphisms in Head and Neck squamous cell carcinoma. Iranian Journal of Basic Medical Sciences. 2011; 14(2):137-144. 7- Faranak Mahmoudi Alami, Mehran Ahmadi, Hamidreza Bazrafshan, Alijan Tabarraei, Ayyoob Khosravi, Mohammad Amin Tabatabaiefar and Nader Mansour Samaei. Association of the TCF7L2 rs12255372 (G/T) variant with type 2 diabetes mellitus in an Iranian population. Genet Mol Biol, 2012; 35(2): 413–417. 8- Faranak Mahmoudi Alami, Nader Mansour Samaei, Mehran Ahmadi, Alijan Tabarraei, Ayyoob Khosravi, Mohammad Amin Tabatabaiefar and Seyyede Naemeh Javid. Associa on of Transcrip on Factor 7-Like 2 (Tcf7l2) Gene Haplotypes with
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the Risk of Type 2 Diabetes Mellitus in Iran. Advances in Biological Research 7 (5): 145-150, 2013. 9- Ghezelsofla E, Tabaraee B, Aghamolaee H, Harzandi N, Sabokbar A, Zamani M, Khosravi A. Optimization of PCR assay for artificially contaminated serum samples as a model for Rapid laboratory confirmation of human brucellosis. Iranian Journal of Infectious Diseases and Tropical Medicine. 2013; 18(61):15-20. 10- Ayyoob Khosravi, Bita Javan ,MohammadAminTabatabaiefar, Hamid Ebadi, Davood Fathi, Majid Shahbazi. Association of interleukin-1 gene cluster polymorphisms and haplotypes with multiple sclerosis in an Iranian population. Journal of Neuroimmunology 288 (2015) 114–119. 11- Ayyoob Khosravi, Masoud Khoshnia, Vahideh Kazeminejad, Jahanbakhsh, Asadi (2016). Authentication of newly established human esophageal squamous cell carcinoma cell line (YM-1) using short tandem repeat (STR) profiling method. Tumor Biology: 37 (3), pp3197-3204. 12- Pedram Torabian, Ayyoob Khosravi, Mehdi Zahedi, Haghjoo Majid, Mehdi Gholizadeh , Vahid Khori. Lack of associa on between KCNE2 gene variants and the congenital long QT syndrome in Iranian population. Physiol Pharmacol 2016, 20(3): 172-178. 13- Reza Abdolmohammadi, Saleh Shahbazi Azar, Ayyoob Khosravi, Majid Shahbazi (2016). CCR5 Polymorphism as a Protec ve Factor for Hepatocellular Carcinoma in Hepatitis B Virus-Infected Iranian Patients. Asian Pac J Cancer Prev, 17 (10), 4643- 4646. 14- Esmaeil Samadian, Ayyoob Khosravi, Roghaye Gharae, Mostafa Mir, Seyed Ahmad
Sajjadi, Fahimeh Mohammad Abadi, Nader Hashemi, Hamid Reza Joshaghani (2016).
Computational Prediction of the Effects of Single Nucleotide Polymorphisms of the Gene
Encoding Human Endothelial Nitric Oxide Synthase. Medical Laboratory Journal. 17 (3), 1- 5.
International conference abstracts: 1- Khosravi Ayyoob, Tavallaii Mahmood, Zeinali Sirus, Sajjadian Abdollah, 2005. Constructing VWA allelic ladder by molecular cloning method for DNA typing purpose. Seoul Korea, ICML 2005.
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2- Sajjadian Abdollah, Khosravi Ayyoob, Tavallaii Mahmood, Zeinali Sirus, 2005. Construc ng THO1 allelic ladder by molecular cloning method for DNA typing purpose. 12th European congress on Biotechnology, August 21-24, 2005. 3- Mirza Khalil Bahmani, Ayyoob Khosravi, Rahele Sheikhi, Fereidoun Mahboodi, Ramin Sarrami-Forooshani. Molecular investigation of anti-retroviral drug resistance in HIV-positive patients from Fars province, Iran. American society for virology, 26th annual mee ng Oregon state university, July 14-18, 2007. 4- Mirza Khalil Bahmani, Ayyoob Khosravi, Fatemeh Saadat. Prevalence and risk factors of hepatitis B virus, hepatitis C virus and human immunodeficiency virus infections among health personnel’s in Gerash hospital, Iran. American society for virology, 27th annual mee ng Cornell University Ithaca New York, July 12-16, 2008. 5- Mirza Khalil Bahmani, Ayyoob Khosravi, Fatemeh Saadat. Hepatitis C Prevalence among patients infected with human immunodeficiency virus infections from Fars Province, southwest of Iran. American society for virology, 28th annual meeting, University of British Columbia Vancouver, BC, Canada, July 11-15, 2009. 6- Mirbehbahani N.B, Rashidbaghan A, Livani S, Khosravi A. Relation between inhibitor produc on and BCLI/Intron 18 and VNTR ST14 polymorphisms in an Iranian population with hemophilia A. 20th Congress of the European Hematology Associa on (Vienna). Hematologica 2015; 100 (s1) abstract n, PB1690.
Domestic Conference abstracts: 1- Ayyoob Khosravi, Mirza Khalil Bahmani, Rahele Sheikhi, 2007. Detection of HIV-1 virus using an indirect immunoflourescence assay (IFA). First Iranian congress of clinical microbiology, May 6-10 2007, Shiraz -Iran. 2- Ayyoob Khosravi ,Mirzakhalil Bahmani, Adel Mobasser, Islam Ghezel Sofla, Fatemeh Saadat. Seroprevalence of Antibodies to Hepatitis B core Antigen (HBc-Antibody) in vaccinated healthcare workers (HCWs). Second congress of clinical microbiology, Oct 7-9 2008, Shiraz-Iran. 3- Mirzakhalil Bahmani, Ayyoob Khosravi, Adel Mobasser, Islam Ghezel Sofla, Mahdi Mohsenzade, Fatemeh Saadat. Detection of Hepatitis B serological markers among anti-HBs negative fully vaccinated health care workers (HCWs). Second congress of clinical microbiology, Oct 7-9 2008, Shiraz-Iran.
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4- Mirzakhalil Bahmani, Ayyoob Khosravi, Adel Mobasser, Islam Ghezel Sofla, Kamran Mobassheri.Hepatitis B Virus Infection among Nurses in Gerash and Evaz Hospitals, Fars Province. Second congress of clinical microbiology, Oct 7-9 2008, Shiraz-Iran. 5- Mirzakhalil Bahmani, Ayyoob Khosravi, Adel Mobasser, Islam Ghezel Sofla, Ghavam Ansari, Ali-Housene Naserinejad. Prevalence of hepatitis C virus (HCV) in human immunodeficiency virus infected. First International congress of AIDS, Nov 12-13 2008, Tehran, Iran. 6- Bahmani M, Hajihoseini S, Khosravi A and Ghezelsofla I .Increased risk of Head and Neck squamous cell carcinoma metastasis in carriers of 5A allele of MMP3 gene promoter. J. Iran. Chem. Soc., Vol. 6, Suppl., November 2009, pp. S203-S229. 7- Abutaleb Mehrabi, Ayyoob Khosravi, Mirza Khalil Bahmani. The prevalence of human immunodeficiency virus (HIV) in cases referred from their physician or health centers to a private clinic in Fars Province.Third congress of clinical microbiology, Oct 6-8 2009, Shiraz-Iran. 8- Ayyoob Khosravi, Mohammad Razeghi, Mirza Khalil Bahmani, Farnaz Farmani. HIV/AIDS -related knowledge and attitudes among students in Shiraz University of medical sciences. Third congress of clinical microbiology, Oct 6-8 2009, Shiraz-Iran. 9- Mirza Khalil Bahmani, Ayyoob Khosravi, Adel Mobasser, Islam Qhezelsofla, Fatemeh Saadat, Kamran Mobasheri. Hepatitis B Virus Vaccination Compliance among Health Care Workers of Gerash and Evaz hospitals, Fars Province. Third congress of clinical microbiology, Oct 6-8 2009, Shiraz-Iran. 10- Ayyoob Khosravi, Mohammad Ali Okhovat. Islam (Shia) and self-plagiarism of academic publishing. 3rd interna onal & 8th national congress on quality improvement in clinical laboratories. Aprill 21-23 2010, Tehran, Iran. 11- Khosravi Ayyoob, Mohammad Ali Okhovat, Zahra Mansourabadi, Mahsa Noori_Madavani, Elham Heidari, Farideh Mohammadian. The Effect of Working and Family Socio-Psychological Environment on Psychological Health of hospital Personnel: Male Subject. Annual research congress of Iranian medical sciences student. Aprill 20-23 2010, Bandar Abbas, Iran. 12- Khosravi Ayyoob, Bahmani Mirza Khalil, Ghezelsofla Eslam, Hajihoseini Samaneh, Piroozi Ali-Yar. Seroprevalence of Hepatitis delta virus (HDV) Infection in HBV-HIV
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co-infected patients of Fars province. 4th Iranian congress of clinical microbiology. november 9-11 2010, Isfahan, Iran. 13- Abdolmohammadi Reza, Ayyoob khosravi, Maryam Mansoori, Bita Javan, Majid Shahbazi. Association between CCR5 polymorphisms and suscep bility to HBV infection in Iranian population. First International congress of molecular medicine 16-19 February 2011, Shiraz, Iran. 14- Javan Bita, Kalani Mohammad Reza, Khosravi Ayyoob, Shahbazi Majid. Polymorphisms of interleukine-1 alpha genes in pa ents with chronic Hepa s B virus. First International congress of molecular medicine 16-19 February 2011, Shiraz, Iran. Oral presentation in international Conference: 1- Khosravi Ayyoob, Bita Javan, Mehrnaz Izadpanah, Majid Shahbazi. Association of IL- 1β gene polymorphisms with mul ple sclerosis in Iranian popula on. Iran MS society, the 9th international MS congress of Iran, Tehran, October 31- November 2, 2012. Oral presentation in Domestic Conference: 1- Ayyoob Khosravi, Mirza Khalil Bahmani, Samaneh Hajihoseini, Aliyar Piroozi, Eslam GhezelSofla. Molecular and phylogenetic analysis of HIV-1 variants circula ng among Fars province, south of Iran. Third congress of clinical microbiology, 6-8 Oct 2009, Shiraz-Iran.
Book translation (English to Farsi): 1- English version: Butler, M. Michael. Animal cell culture and technology. 2ed ed 2004.
ﺗﺮﺟﻤﻪ ﮐﺘﺎب ﻓﺎرﺳﯽ ﺑﺎ ﻋﻨﻮان : ﻓﻨﺎوري ﮐﺸﺖ ﺳﻠﻮل ﻫﺎي ﺣﯿﻮاﻧﯽ، اﻧﺘﺸﺎرات داﻧﺸﮕﺎه اﻣﺎم ﺣﺴﯿﻦ (ع)، 1385.
Gene recording in: National Center for Biotechnology Information, NCBI: Address: http://www.ncbi.nlm.nih.gov→nucleo de→Accession number Number Env Gene of Code of genes genotype Accession year HIV-1 virus number in NCBI
1 c2v3c3 region HIV-1 isolate A1 Subtype A (A1) EU881931 23-JUL-2008 2 √ HIVseq-2 Subtype A (A1) FJ178375 29-SEP-2008
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3 √ HIVseq-3 Subtype A (A1) FJ178376 29-SEP-2008 4 √ HIVseq-4 Subtype A (A1) FJ807630 04-MAY-2009 5 √ HIVseq-5 Subtype A (A1) FJ790242 09-MAY-2009 6 √ HIVseq-6 Subtype A (A1) FJ392755 04-MAY-2009 7 √ HIVseq-7 Subtype A (A1) GQ243705 28-JUL-2009 8 √ HIVseq-8 Subtype A (A1) GQ243706 28-JUL-2009 9 √ HIVseq-9 Subtype A (A1) GQ243707 28-JUL-2009 10 √ HIVseq-10 Subtype A (A1) GQ243708 28-JUL-2009 11 √ HIVseq-11 Subtype A (A1) GQ853425 26-OCT-2009 12 √ HIVseq-12 Subtype A (A1) GQ853426 26-OCT-2009 13 √ HIVseq-13 Subtype A (A1) GQ853427 26-OCT-2009 14 √ HIVseq-14 Subtype A (A1) GQ853428 26-OCT-2009
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