THE MICROSCOPE a Practical Guide
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Use of Laboratory Equipement
USE OF LABORATORY EQUIPEMENT C. Laboratory Thermometers Most thermometers are based upon the principle that liquids expand when heated. Most common thermometers use mercury or colored alcohol as the liquid. These thermometers are constructed as that a uniform-diameter capillary tube surmounts a liquid reservoir. To calibrate a thermometer, one defines two reference points, normally the freezing point of water (0°C, 32°F) and the boiling point of water (100°C, 212°F) at 1 tam of pressure (1 tam = 760 mm Hg). Once these points are marked on the capillary, its length is then subdivided into uniform divisions called degrees. There are 100° between these two points on the Celsius (°C, or centigrade) scale and 180° between those two points on the Fahrenheit (°F) scale. °F = 1.8 °C + 32 The Thermometer and Its Calibration This section describes the proper technique for checking the accuracy of your thermometer. These measurements will show how measured temperatures (read from thermometer) compare with true temperatures (the boiling and freezing points of water). The freezing point of water is 0°C; the boiling point depends upon atmospheric pressure but at sea level it is 100°C. Option 1: Place approximately 50 mL of ice in a 250-mL beaker and cover the ice with distilled water. Allow about 15 min for the mixture to come to equilibrium and then measure and record the temperature of the mixture. Theoretically, this temperature is 0°C. Option 2: Set up a 250-mL beaker on a wire gauze and iron ring. Fill the beaker about half full with distilled water. -
Laboratory Glassware N Edition No
Laboratory Glassware n Edition No. 2 n Index Introduction 3 Ground joint glassware 13 Volumetric glassware 53 General laboratory glassware 65 Alphabetical index 76 Índice alfabético 77 Index Reference index 78 [email protected] Scharlau has been in the scientific glassware business for over 15 years Until now Scharlab S.L. had limited its sales to the Spanish market. However, now, coinciding with the inauguration of the new workshop next to our warehouse in Sentmenat, we are ready to export our scientific glassware to other countries. Standard and made to order Products for which there is regular demand are produced in larger Scharlau glassware quantities and then stocked for almost immediate supply. Other products are either manufactured directly from glass tubing or are constructed from a number of semi-finished products. Quality Even today, scientific glassblowing remains a highly skilled hand craft and the quality of glassware depends on the skill of each blower. Careful selection of the raw glass ensures that our final products are free from imperfections such as air lines, scratches and stones. You will be able to judge for yourself the workmanship of our glassware products. Safety All our glassware is annealed and made stress free to avoid breakage. Fax: +34 93 715 67 25 Scharlab The Lab Sourcing Group 3 www.scharlab.com Glassware Scharlau glassware is made from borosilicate glass that meets the specifications of the following standards: BS ISO 3585, DIN 12217 Type 3.3 Borosilicate glass ASTM E-438 Type 1 Class A Borosilicate glass US Pharmacopoeia Type 1 Borosilicate glass European Pharmacopoeia Type 1 Glass The typical chemical composition of our borosilicate glass is as follows: O Si 2 81% B2O3 13% Na2O 4% Al2O3 2% Glass is an inorganic substance that on cooling becomes rigid without crystallising and therefore it has no melting point as such. -
PML Brochure
PHYSICAL MEASUREMENT LABORATORY Gauging nature on all scales NIST.GOV/PML PHYSICAL MEASUREMENT LABORATORY (PML) The Physical Measurement Laboratory (PML), a major frequency, electricity, temperature, humidity, pressure operating unit of the National Institute of Standards and vacuum, liquid and gas flow, and electromag- and Technology (NIST), sets the definitive U.S. standards netic, optical, acoustic, and ionizing radiation. PML for nearly every kind of measurement in modern life, collaborates directly with industry, universities, profes- sometimes across more than 20 orders of magni- sional and standards-setting organizations, and other tude. PML is a world leader in the science of physical agencies of government to ensure accuracy and to measurement, devising procedures and tools that make solve problems. It also supports research in many continual progress possible. Exact measurements are fields of urgent national importance, such as manu- absolutely essential to industry, medicine, the research facturing, energy, health, law enforcement and community, and government. All of them depend on homeland security, communications, military defense, PML to develop, maintain, and disseminate the official electronics, the environment, lighting and display, standards for a wide range of quantities, including radiation, remote sensing, space exploration, length, mass, force and shock, acceleration, time and and transportation. IMPACTS ❱ Provides 700 kinds of calibration services ❱ Numerous special testing services NIST.GOV/PML | 2 NIST.GOV/PML -
Chemostat Culture for Yeast Experimental Evolution
Downloaded from http://cshprotocols.cshlp.org/ at Cold Spring Harbor Laboratory Library on August 9, 2017 - Published by Cold Spring Harbor Laboratory Press Protocol Chemostat Culture for Yeast Experimental Evolution Celia Payen and Maitreya J. Dunham1 Department of Genome Sciences, University of Washington, Seattle, Washington 98195 Experimental evolution is one approach used to address a broad range of questions related to evolution and adaptation to strong selection pressures. Experimental evolution of diverse microbial and viral systems has routinely been used to study new traits and behaviors and also to dissect mechanisms of rapid evolution. This protocol describes the practical aspects of experimental evolution with yeast grown in chemostats, including the setup of the experiment and sampling methods as well as best laboratory and record-keeping practices. MATERIALS It is essential that you consult the appropriate Material Safety Data Sheets and your institution’s Environmental Health and Safety Office for proper handling of equipment and hazardous material used in this protocol. Reagents Defined minimal medium appropriate for the experiment For examples, see Protocol: Assembly of a Mini-Chemostat Array (Miller et al. 2015). Ethanol (95%) Glycerol (20% and 50%; sterile) Yeast strain of interest Equipment Agar plates (appropriate for chosen strain) Chemostat array Assemble the apparatus as described in Miller et al. (2013) and Protocol: Assembly of a Mini-Chemostat Array (Miller et al. 2015). Cryo deep-freeze labels Cryogenic vials Culture tubes Cytometer (BD Accuri C6) Glass beads, 4 mm (sterile; for plating yeast cells) Glass cylinder Kimwipes 1Correspondence: [email protected] © 2017 Cold Spring Harbor Laboratory Press Cite this protocol as Cold Spring Harb Protoc; doi:10.1101/pdb.prot089011 559 Downloaded from http://cshprotocols.cshlp.org/ at Cold Spring Harbor Laboratory Library on August 9, 2017 - Published by Cold Spring Harbor Laboratory Press C. -
Manufacturing Establishments Under the Fair Labor Standards Act (FLSA)
U.S. Department of Labor Wage and Hour Division (Revised July 2008) Fact Sheet #9: Manufacturing Establishments Under the Fair Labor Standards Act (FLSA) This fact sheet provides general information concerning the application of the FLSA to manufacturers. Characteristics Employees who work in manufacturing, processing, and distributing establishments (including wholesale and retail establishments) that produce, handle, or work on goods for interstate or foreign commerce are included in the category of employees engaged in the production of goods for commerce. The minimum wage and overtime pay provisions of the Act apply to employees so engaged in the production of goods for commerce. Coverage The FLSA applies to employees of a manufacturing business covered either on an "enterprise" basis or by "individual" employee coverage. If the manufacturing business has at least some employees who are "engaged in commerce" and meet the $500,000 annual dollar volume test, then the business is required to pay all employees in the "enterprise" in compliance with the FLSA without regard to whether they are individually covered. A business that does not meet the dollar volume test discussed above may still be required to comply with the FLSA for employees covered on an "individual" basis if any of their work in a workweek involves engagement in interstate commerce or the production of goods for interstate commerce. The concept of individual coverage is indeed broad and extends not only to those employees actually performing work in the production of goods to be directly shipped outside the State, but also applies if the goods are sold to a customer who will ship them across State lines or use them as ingredients of goods that will move in interstate commerce. -
Resolving Power
The 5 I’s of Culturing Microbes • Inoculation • Isolation • Incubation • Inspection • Identification 8/18/12 MDufilho 1 Table 4.1 Metric Units of Length 8/18/12 MDufilho 2 Microscopy • General Principles of Microscopy – Wavelength of radiation – Magnification – Resolution – Contrast 8/18/12 MDufilho 3 Figure 4.1 The electromagnetic spectrum 400 nm 700 nm Visible light X UV Infra- Micro- Radio waves and Gamma rays rays light red wave Television Increasing wavelength 10–12m 10–8m 10–4m 100m 103m Crest One wavelength Trough Increasing resolving power 8/18/12 MDufilho 4 Figure 4.2 Light refraction and image magnification by a convex glass lens-overview Light Air Glass Focal point Specimen Convex Inverted, lens reversed, and enlarged 8/18/12 MDufilho image 5 Principles of Light Microscopy • Magnification occurs in two phases – – The objective lens forms the magnified real image – The real image is projected to the ocular where it is magnified again to form the virtual image • Total magnification of the final image is a product of the separate magnifying powers of the two lenses power of objective x power of ocular = total magnification 8/18/12 MDufilho 6 8/18/12 MDufilho 7 Resolution Resolution defines the capacity to distinguish or separate two adjacent objects – resolving power – Function of wavelength of light that forms the image along with characteristics of objectives • Visible light wavelength is 400 nm–750 nm • Numerical aperture of lens ranges from 0.1 to 1.25 • Oil immersion lens requires the use of oil to prevent refractive loss -
BROOKFIELD DIAL READING VISCOMETER with Electronic Drive
BROOKFIELD DIAL READING VISCOMETER with Electronic Drive Operating Instructions Manual No. M00-151-I0614 SPECIALISTS IN THE MEASUREMENT AND CONTROL OF VISCOSITY with offices in : Boston • Chicago • London • Stuttgart • Guangzhou BROOKFIELD ENGINEERING LABORATORIES, INC. 11 Commerce Boulevard, Middleboro, MA 02346 USA TEL 508-946-6200 or 800-628-8139 (USA excluding MA) FAX 508-946-6262 INTERNET http://www.brookfieldengineering.com TABLE OF CONTENTS I. INTRODUCTION .....................................................................................5 I.1 Components .......................................................................................................5 I.2 Utilities ................................................................................................................6 I.3 Specifications .....................................................................................................6 I.4 Set-Up ................................................................................................................7 I.5 IQ, OQ, PQ .........................................................................................................7 I.6 Safety Symbols and Precautions .......................................................................8 I.7 Cleaning .............................................................................................................8 II. GETTING STARTED ..............................................................................9 II.1 Operation ...........................................................................................................9 -
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position. -
Human Activity Analysis and Recognition from Smartphones Using Machine Learning Techniques
Human Activity Analysis and Recognition from Smartphones using Machine Learning Techniques Jakaria Rabbi, Md. Tahmid Hasan Fuad, Md. Abdul Awal Department of Computer Science and Engineering Khulna University of Engineering & Technology Khulna-9203, Bangladesh jakaria [email protected], [email protected], [email protected] Abstract—Human Activity Recognition (HAR) is considered a HAR is the problem of classifying day-to-day human ac- valuable research topic in the last few decades. Different types tivity using data collected from smartphone sensors. Data are of machine learning models are used for this purpose, and this continuously generated from the accelerometer and gyroscope, is a part of analyzing human behavior through machines. It is not a trivial task to analyze the data from wearable sensors and these data are instrumental in predicting our activities for complex and high dimensions. Nowadays, researchers mostly such as walking or standing. There are lots of datasets and use smartphones or smart home sensors to capture these data. ongoing research on this topic. In [8], the authors discuss In our paper, we analyze these data using machine learning wearable sensor data and related works of predictions with models to recognize human activities, which are now widely machine learning techniques. Wearable devices can predict an used for many purposes such as physical and mental health monitoring. We apply different machine learning models and extensive range of activities using data from various sensors. compare performances. We use Logistic Regression (LR) as the Deep Learning models are also being used to predict various benchmark model for its simplicity and excellent performance on human activities [9]. -
Inside the Video Game Industry
Inside the Video Game Industry GameDevelopersTalkAbout theBusinessofPlay Judd Ethan Ruggill, Ken S. McAllister, Randy Nichols, and Ryan Kaufman Downloaded by [Pennsylvania State University] at 11:09 14 September 2017 First published by Routledge Th ird Avenue, New York, NY and by Routledge Park Square, Milton Park, Abingdon, Oxon OX RN Routledge is an imprint of the Taylor & Francis Group, an Informa business © Taylor & Francis Th e right of Judd Ethan Ruggill, Ken S. McAllister, Randy Nichols, and Ryan Kaufman to be identifi ed as authors of this work has been asserted by them in accordance with sections and of the Copyright, Designs and Patents Act . All rights reserved. No part of this book may be reprinted or reproduced or utilised in any form or by any electronic, mechanical, or other means, now known or hereafter invented, including photocopying and recording, or in any information storage or retrieval system, without permission in writing from the publishers. Trademark notice : Product or corporate names may be trademarks or registered trademarks, and are used only for identifi cation and explanation without intent to infringe. Library of Congress Cataloging in Publication Data Names: Ruggill, Judd Ethan, editor. | McAllister, Ken S., – editor. | Nichols, Randall K., editor. | Kaufman, Ryan, editor. Title: Inside the video game industry : game developers talk about the business of play / edited by Judd Ethan Ruggill, Ken S. McAllister, Randy Nichols, and Ryan Kaufman. Description: New York : Routledge is an imprint of the Taylor & Francis Group, an Informa Business, [] | Includes index. Identifi ers: LCCN | ISBN (hardback) | ISBN (pbk.) | ISBN (ebk) Subjects: LCSH: Video games industry. -
Rhodamine B Lipase/Esterase Agar
ALKEN-MURRAY CORPORATION TI TLE: RHODA M INE B A GA R NO. QC-99 PREPARA TI ON PROCEDURE MICROBIOLOGICAL DIVISION QUALITY CONTROL REV : 3 PAGE 1 OF 4 ALKEN-MURRAY CORPORATION P. O. Box 400, New Hyde Park, NY 11040 TELEPHONE 540-636-1236 - Fax 540-636-1770 QUALITY CONTROL METHOD - 99 Preparation and Use of Rhodamine B Lipase Agar Description: This quality control procedure is designed to verify that Rhodamine B Agar is properly prepared, used and stored. This procedure demonstrates the ability of some bacteria to produce lipase and degrade food oils, such as olive oil, in a clear and easily interpreted manner. We found that the Spirit Blue method (QC-94) was harder to interpret, leading to misinterpreted results. This procedure should be performed by a trained laboratory technician. Ingredients: For lipoidal emulsion (50 ml per 450 ml of base medium) - enough lipoidal emulsion for 1 liter: Olive oil 30 ml Tween 80 250 µl Distilled water 50 ml 1 Rhodamine B 20 mg/20 ml sterile water (0.02% w/v total media prepared) 1. Add filter sterilized Rhodamine B solution, after autoclaving other ingredients For base medium: Nutrient Broth 4.5 g (alternate Peptone water) Yeast extract 1.25 g Agar 10 g Distilled water 450 ml Final pH 7.0 ± 0.2 Equipment and Supplies: 500 ml Graduated cylinder 50 ml Graduated cylinder 1 ml sterile syringe with 18 gauge needle 10 ml sterile syringe with 18 gauge needle 10 ml sterile serological pipet Drummond Pipet-Aid® or rubber bulb 2 - 50 ml sterile serological pipets ALKEN-MURRAY CORPORATION TI TLE: RHODA M INE B A GA R NO. -
Practical Tips for Two-Photon Microscopy
Appendix 1 Practical Tips for Two-Photon Microscopy Mark B. Cannell, Angus McMorland, and Christian Soeller INTRODUCTION blue and green diode lasers. To provide an alignment beam to which the external laser can be aligned, light from this reference As is clear from a number of the chapters in this volume, 2-photon laser needs to be bounced back through the microscope optical microscopy offers many advantages, especially for living-cell train and out through the external coupling port: studies of thick specimens such as brain slices and embryos. CAUTION: Before you switch on the reference laser in this However, these advantages must be balanced against the fact that configuration make sure that all PMTs are protected and/or commercial multiphoton instrumentation is much more costly than turned off. the equipment used for confocal or widefield/deconvolution. Given Place a front-surface mirror on the stage of the microscope and these two facts, it is not surprising that, to an extent much greater focus onto the reflective surface using an air objective for conve- than is true of confocal, many researchers have decided to add a nience (at sharp focus, you should be able to see scratches or other femtosecond (fs) pulsed near-IR laser to a scanner and a micro- mirror defects through the eyepieces). The idea of this method is scope to make their own system (Soeller and Cannell, 1996; Tsai to cause the reference laser beam to bounce back through the et al., 2002; Potter, 2005). Even those who purchase a commercial optical train and emerge from the other laser port.