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GSTK1 Rabbit pAb

Catalog No.: A5226 1 Publications

Basic Information Background

Catalog No. This encodes a member of the kappa class of the glutathione transferase A5226 superfamily of that function in cellular detoxification. The encoded protein is localized to the and catalyzes the conjugation of glutathione to a wide Observed MW range of hydrophobic substates facilitating the removal of these compounds from cells. 25KDa Alternative splicing results in multiple transcript variants.

Calculated MW 20kDa/24kDa/25kDa/31kDa

Category Primary antibody

Applications WB, IHC, IF

Cross-Reactivity Human, Mouse, Rat

Recommended Dilutions Immunogen Information

WB 1:500 - 1:2000 Gene ID Swiss Prot 373156 Q9Y2Q3 IHC 1:50 - 1:100 Immunogen 1:50 - 1:100 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human GSTK1 (NP_057001.1).

Synonyms GSTK1;GST;GST 13-13;GST13;GST13-13;GSTK1-1;hGSTK1

Contact Product Information

www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification

Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data

Western blot analysis of extracts of various cell lines, using GSTK1 antibody (A5226) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded human lung using embedded human liver cancer using embedded human thyroid cancer using GSTK1 Rabbit pAb (A5226) at dilution of GSTK1 Rabbit pAb (A5226) at dilution of GSTK1 Rabbit pAb (A5226) at dilution of 1:25 (40x lens). 1:25 (40x lens). 1:25 (40x lens).

Immunofluorescence analysis of Hela cells using GSTK1 antibody (A5226) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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