DOCSLIB.ORG

United States Patent (10) Patent No.: US 7,897,791 B2 Seeram Et Al

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
United States Patent (10) Patent No.: US 7,897,791 B2 Seeram Et Al US007897791 B2 (12) United States Patent (10) Patent No.: US 7,897,791 B2 Seeram et al. (45) Date of Patent: *Mar. 1, 2011 (54) PURIFICATIONS OF POMEGRANATE OTHER PUBLICATIONS ELLAGTANNINS AND THEIR USES Aviram, M., et al., “Pomegranate juice consumption inhibits serum THEREOF angiotensin converting enzyme activity and reduces systolic blood pressure.” (2001) Atherosclerosis, 158: 195-198. (75) Inventors: Navindra P. Seeram, Kingston, RI (US); Cerda, B., et al., “Evaluation of bioavailability and metabolism in the David Heber, Los Angeles, CA (US) rat of punicalagin, an antioxidant polyphenol from pomegranate juice.” (2003) Eur, J. Nutr., 42:18-28. (73) Assignee: The Regents of the University of Cerda, B., et al., “Repeated oral administration of high doses of the California, Oakland, CA (US) pomegranate elagitannin punicalaginto rats for 37 days is not toxic.” (2003) J. Agric. Food Chem. 51:3493-3501. (*) Notice: Subject to any disclaimer, the term of this Doig, A., et al., “Isolation and structure elucidation of punicalagin, a patent is extended or adjusted under 35 toxic hydrolysable tannin, from Terminalia oblongata.” (1990) J. U.S.C. 154(b) by 0 days. Chem. Soc. Perkin Trans. I, 2317-2321. EL-Toumy, S., et al., “Two ellagitannins from Punica granatum This patent is Subject to a terminal dis heartwood.” (2002) Phytochemistry, 61:971-974. claimer. Filippich, L., et al., “Hepatotoxic and nephrotoxic principles in Terminalia oblongata.” (1991) Research in Veterinary Science, Appl. No.: 12/647,229 50:17O-177. (21) Gil, M., et al., “Antioxidant activity of pomegranate juice and its Filed: Dec. 24, 2009 relationship with phenolic composition and processing.” (2000) J. (22) Agric. Food Chem., 48:4581-4589. Mayer, et al., “Punicalagin and punicalin, two tannins from pome (65) Prior Publication Data granate peel.” (1977) Liebigs Ann. Chem, 1976-1986. US 2010/O1 7386O A1 Jul. 8, 2010 Negi, P. et al., “Antioxidant and antimutagenic activities of pome granate peel extracts.” (2003) Food Chemistry, 80:393-397. Related U.S. Application Data Scalbert, A., et al., “Absorption and metabolism of polyphenols in the gut and impact on health.” (2002) Biomed. Pharmacother, 56:276 (60) Division of application No. 1 1/395,447, filed on Mar. 282. 30, 2006, now Pat. No. 7,638,640, which is a continu Tanaka; et al., “Punicafolin, an Ellagitannin from the Leaves of ation-in-part of application No. PCT/US2005/009337, Punica granatum'. Phytochemistry (1985), 24 (9): 2075-2078. filed on Mar. 21, 2005. Tanaka, T., et al., “Tannins and related compounds XL. Revision of the structures of punicalin and punicalagin, and isolation and (60) Provisional application No. 60/556,322, filed on Mar. characterizatin of 2-O-Galloylpunacalin from the bark of Punica 24, 2004. granatum L.” (1986) Chem. Pharm. Bull., 34(2): 650-655. Tanaka, T., et al., “Tannins and related compounds. XLL. Isolation (51) Int. C. and characterization of novel ellagitannins, punicacorteins A, B, C CO7D 49.3/00 (2006.01) and D, and punigluconin from the bark of punica granatum L.” AOIK 43/04 (2006.01) (1986) Chem. Pharm. Bull., 34 (2):656-663. AOIN 43/42 (2006.01) Vidal, A., et al., “Studies on the toxicity of Punica granatum . AOIN 43/16 (2006.01) (Punicaceae) whole fruit extracts.” (2003) Journal of (52) U.S. Cl. ....................... 549/278: 514/292; 514/453; Ethnopharmacology, 89:295-300. 514/456; 514/27 * cited by examiner (58) Field of Classification Search ................. 549/278; 514/27, 292, 453, 456 Primary Examiner Nizal S Chandrakumar See application file for complete search history. (74) Attorney, Agent, or Firm—Bozicevic, Field & Francis LLP: Pamela J. Sherwood (56) References Cited (57) ABSTRACT U.S. PATENT DOCUMENTS 6,387,418 B1 5, 2002 Aviram et al. Compositions of purified and biologically active ellagitan 6,641,850 B1 1 1/2003 Aviram et al. nins are provided by separation from pomegranate husk using 6,977,089 B1 12/2005 Aviram et al. a method of extraction and purification using a solid poly 7,638,640 B2 * 12/2009 Seeram et al. .............. 549,278 meric adsorbent and the uses of the said compounds. FOREIGN PATENT DOCUMENTS JP 61100517 A 5, 1986 13 Claims, 4 Drawing Sheets U.S. Patent Mar. 1, 2011 Sheet 1 of 4 US 7,897,791 B2 5.0 100 15.0 0.00 25.0 30,0 350 4.O.O 45 Minute 5.0 O.O 15.0 2O.O 25.0 3O.O 35.0 400 45 Minute 5.O 10.0 15,0 20.0 25.0 3O.O 35.0 40.O 45 Minute U.S. Patent Mar. 1, 2011 Sheet 2 of 4 US 7,897,791 B2 U.S. Patent Mar. 1, 2011 Sheet 3 of 4 US 7,897,791 B2 Elagic acid (4) (mz301) Punicalagin (1) (miz1083) Gallagic acid (3) (miz601) Punicalin (2) (mz781) Figure 3A U.S. Patent Mar. 1, 2011 Sheet 4 of 4 US 7,897,791 B2 7812 | 5 O 30 a. 20 5 549.1 575.2 538 a 3932 1064.5 3374 930.9 988.9 O -- el, DeCD D D O D D 300 400 500 600 700 800 900 1000 1100 1200 Figure 3B US 7,897,791 B2 1. 2 PURIFICATIONS OF POMEGRANATE pounds, the development of inexpensive, high throughput ELLAGTANNINS AND THEIR USES methods of purificationis of particular interest. THEREOF SUMMARY OF THE INVENTION CROSS REFERENCE TO RELATED Compositions of purified and biologically active ellagitan APPLICATION(S) nins, specifically including punicalagin and gallagic acid are provided, as well as methods for producing Such composi This application is a divisional non-provisional patent tions and their uses. Ellagitannins are separated from other application of Ser. No. 1 1/395,447 (now allowed) filed before 10 pomegranate natural products by a method of extraction and the U.S. Patent & Trademark Office (USPTO) on Mar. 30, purification using a series of solvent-solvent extractions and 2006, which is in turn a continuation-in-part application of chromatography with solid polymeric adsorbents. Included PCT application No. PCT/US2005/009337 filed before the in the compositions of the invention are extracts comprising USPTO receiving office on Mar. 21, 2005, which claims gallagic acidata concentration of at least about 0.1% or more, 15 up to Substantially pure gallagic acid. Also included in the priority to U.S. provisional patent application of Ser. No. compositions of the invention are extracts comprising puni 60/556,322 filed before the USPTO on Mar. 24, 2004. This calagin at a concentration of at least about 0.1% or more, up divisional application incorporates by reference in its entirety to Substantially pure punicalagin. Compositions are also pro each of the foregoing patent applications (i.e., non-provi vided comprising elagic acid and ellagic acid glycosides at a sional patent application of Ser. No. 1 1/395,447; continua concentration of at least about 5% or more. In another tion-in-part application of PCT application no. PCT/US05/ embodiment, compositions are provided comprising antho 09337; and U.S. provisional patent application of Ser. No. cyanins and anthocyanidins at a concentration of at least 60/556,322. about 0.1%, about 5%, or more. Extracts comprising these compounds, and extracts comprising total pomegranate tan GOVERNMENT RIGHT(S) 25 nins, are useful for a variety of applications, including phar maceutical, neutraceutical, cosmetic, and food uses. In one embodiment of the invention, dried pomegranate This invention was made with Government support of peels are ground and Suspended with an alcohol Such as Grant No. AT000151 awarded by the National Institutes of methanol. A rapid polymeric adsorbent column is used to Health. The government has certain rights in this invention. 30 purify pomegranate tannins from an aqueous or alcoholic Recent research has shown that pomegranate (Punica extract, which extract is derived from a source of elagitan granatum L.) juice and pomegranate extracts exhibit potent nins such as a variety of plant extracts, including Punica biological properties attributable to the presence of polyphe granatum L., and the like. The tannins are adsorbed to the nols known as elagitannins. These hydrolysable tannins are columns, and followed by cleansing, gravity evacuation, and present in high levels in pomegranates and include punicala 35 vacuum aspiration of fluid from the column, the adsorbed gin anomers commonly referred to as punicalagins punicalin tannins are eluted from the adsorbent solid and the solvent gallagic acid and elagic acid Pomegranate elagitannins have removed in vacuo to yield a powder of highly concentrated been identified as the active antioxidant compounds respon total tannins comprising a high percentage (e.g., 80%) of sible for protecting low-density lipoprotein cholesterol from punicalagin anomers, as well as a significant percentage of oxidation in Vivo, a key step in the pathogenesis of athero 40 ellagic acid. Additional chromatography steps may be per Sclerosis. The punicalagins are key compounds responsible formed to provide for highly purified compositions of gal for the antioxidant properties of pomegranate extracts. How lagic acid or punicalagin. ever, research has shown that the synergistic combination of The present invention also provides a method for produc punicalagins with other minor pomegranate elagitannins, ing a mixture comprising ellagitannins from pomegranate. 45 The method may include the use of enzymes Such as extrac specifically punicalin and gallagic acid, results in Superior tase and pectinase. The method may include the steps of biological activities. Unfortunately, there are no methods cur providing an aqueous solution containing the ellagitannins rently available for rapid and large scale production of these from the pomegranate; removing the elagitannins onto a minor pomegranate elagitannins. This invention proposes a resin Surface from the aqueous solution; eluting the resin method to produce ellagitannins from pomegranates and out 50 Surface with a eluant to remove the ellagitannins from the lines their uses.
Load more

Recommended publications
  • (12) United States Patent (10) Patent No.: US 7,919,636 B2 Seeram Et Al
    USOO7919636B2 (12) United States Patent (10) Patent No.: US 7,919,636 B2 Seeram et al. (45) Date of Patent: Apr. 5, 2011 (54) PURIFICATIONS OF POMEGRANATE Aviram, M., et al., “Pomegranate juice consumption inhibits serum ELLAGTANNINS AND THEIR USES angiotensin converting enzyme activity and reduces systolic blood THEREOF pressure.” (2001) Atherosclerosis, 158: 195-198. Cerda, B., et al., “Evaluation of bioavailability and metabolism in the (75) Inventors: Navindra P. Seeram, Los Angeles, CA rat of punicalagin, an antioxidant polyphenol from pomegranate juice.” (2003) Eur, J. Nutr., 42:18-28. (US); David Heber, Los Angeles, CA Cerda, B., et al., “Repeated oral administration of high doses of the (US) pomegranate elagitannin punicalaginto rats for 37 days is not toxic.” (2003) J. Agric. Food Chem. 51:3493-3501. (73) Assignee: The Regents of the University of Doig, A., et al., “Isolation and structure elucidation of punicalagin, a California, Oakland, CA (US) toxic hydrolysable tannin, from Terminalia oblongata.” (1990) J. Chem. Soc. Perkin Trans. I, 2317-2321. (*) Notice: Subject to any disclaimer, the term of this El-Toumy, S., et al., “Two ellagitannins from Punica granatum patent is extended or adjusted under 35 heartwood.” (2002) Phytochemistry, 61:971-974. U.S.C. 154(b) by 248 days. Filippich, L., et al., “Hepatotoxic and nephrotoxic principles in Terminalia oblongata.” (1991) Research in Veterinary Science, (21) Appl. No.: 12/143,657 50:17O-177. Gil, M., et al., “Antioxidant activity of pomegranate juice and its (22) Filed: Jun. 20, 2008 relationship with phenolic composition and processing.” (2000) J. Agric. Food Chem., 48:4581-4589.
    [Show full text]
  • In Pomegranates – J
    OPTIONS OPTIONS méditerranéennes méditerranéennes II International Symposium SERIES A: Mediterranean Seminars 2012 – Number 103 CIHEAM on the Pomegranate II International Symposium Edited by: P. Melgarejo, D. Valero on the Pomegranate Edited by: P. Melgarejo, D. Valero These Proceedings include 63 scientific papers presented at the II International Symposium on the Pomegranate, held in Madrid (Spain) from 19 to 21 October 2011. Production and commerce of pomegranate and its derived products have experienced significant growth everywhere in the world. Progress has been made in cultivation techniques and some traditional problems have been solved, although new problems such as Xanthomonas axonopodis attacks constitute a present threat. New public and private institutions in different countries are working to improve the on the Pomegranate production and quality of the pomegranate, and new varieties are being commercialized. The cultivar is a determining factor in issues related to nutritional composition and bioactive compounds with II International Symposium antioxidant activity. At the same time, significant advances in postharvest technologies have been made in the past years, specially focused on the use of technologies with natural compounds of great efficiency in maintaining OPTIONS OPTIONS méditerranéennes the quality and safety of pomegranate fruit during prolonged storage periods. méditerranéennes Special importance is attached to the advance in the industrialization processes through the technologies for processing ready-to-eat arils. In the past years, new protocols for quality evaluation using sensory analysis have been developed. With respect to the relationship between pomegranate and health, recent clinical studies report that pomegranate juice or extract can reduce the progression of prostate cancer, although more studies are needed to confirm these experimental results.
    [Show full text]
  • Journal of Drug Delivery and Therapeutics Punica Granatum L
    Kumari et al Journal of Drug Delivery & Therapeutics. 2021; 11(3):113-121 Available online on 15.05.2021 at http://jddtonline.info Journal of Drug Delivery and Therapeutics Open Access to Pharmaceutical and Medical Research © 2011-21, publisher and licensee JDDT, This is an Open Access article which permits unrestricted non-commercial use(CC By-NC), provided the original work is properly cited Open Access Full Text Article Review Article Punica granatum L. (Dadim), Therapeutic Importance of World’s Most Ancient Fruit Plant Kumari Isha, Kaurav Hemlata, Chaudhary Gitika* Shuddhi Ayurveda, Jeena Sikho Lifecare Pvt. Ltd. Zirakpur, 140603, Punjab, India Article Info: Abstract ___________________________________________ ______________________________________________________________________________________________________ Article History: The custom of using plants for the therapeutic and dietary practices is as old as origin of Received 23 March 2021; humanity on the earth. One of the most ancient fruit plant is Punica granatum L., Review Completed 20 April 2021 pomegranate belongs to Lythraceae family. The plant has a very rich ethnic history of its Accepted 26 April 2021; utilization around the world. The plant was used to symbolize prosperity, life, happiness, Available online 15 May 2021 fertility etc. Apart from the ethnic beliefs associated with the plant, it is a well-considered ______________________________________________________________ plant based remedy used in treatment of many diseases in traditional system like Ayurveda Cite this article as: and folk system of medicine. In Ayurveda it is esteemed as a Rasayana. It is used in many Ayurvedic polyherbal formulations which are used against many diseases. The plant Kumari I, Kaurav H, Chaudhary G, Punica granatum L. (Dadim), Therapeutic Importance of World’s Most consists of numerous phytochemical constituents in it such as polysaccharides, minerals, Ancient Fruit Plant, Journal of Drug Delivery and polyphenols, tannins, saponins, quinones, alkaloids, glycosides, coumarins, terpenoids, Therapeutics.
    [Show full text]
  • Thowiba Mohammed Eltayeb Mohammed
    Phytochemical Analysis and Antimicrobial Activities of Garad (Acacia nilotica L.) and Rumman (Punica granatum L.) Plants Thowiba Mohammed Eltayeb Mohammed B. Sc.( Honor) in Biology and Chemistry, Faculty of Education- Elhasahiesa, University of Gezira (2002) Post- graduate Diploma in Biosciences and Biotechnology, Center of Biosciences and Biotechnology, Faculty of Engineering and Technology (2004) M.sc in Biosciences and Biotechnology, Center of Biosciences and Biotechnology, Faculty of Engineering and Technology (Biosciences) (2009) A Thesis Submitted to the University of Gezira in Fulfillment of the Requirement for the Award of the Degree of Doctor of Philosophy in Biosciences and Biotechnology, (Microbiology) Center of Biosciences and Biotechnology Faculty of Engineering and Technology August 2015 1 Phytochemical Analysis and Antimicrobial Activities of Garad (Acacia nilotica L.) and Rumman (Punica granatum L.) Plants Thowiba Mohammed Eltayeb Mohammed Supervision Committee: Name Position Signature Prof / Awad Mohamed Abdel-Rahim Main Supervisor ………………. Dr. Mutaman Ali Abdelgadir Kehail Co – Supervisor ……………… Date: August, 2015 2 Phytochemical Analysis and Antimicrobial Activities of Garad (Acacia nilotica L) and Rumman (Punica granatum L.) Plants Thowiba Mohammed Eltayeb Mohammed Examination Committee: Name Position Signature Prof/ Awad Mohamed Abdel-Rahim Chairman --------------- Hassan Beshir ElAmin External Examiner -------------- Dr. Zakaria Ahmed Salih Internal Examiner --------------- Date of Examination 10 /08 /2015 3 بسم هللا
    [Show full text]
  • Pomegranate (Punica Granatum)
    Functional Foods in Health and Disease 2016; 6(12):769-787 Page 769 of 787 Research Article Open Access Pomegranate (Punica granatum): a natural source for the development of therapeutic compositions of food supplements with anticancer activities based on electron acceptor molecular characteristics Veljko Veljkovic1,2, Sanja Glisic2, Vladimir Perovic2, Nevena Veljkovic2, Garth L Nicolson3 1Biomed Protection, Galveston, TX, USA; 2Center for Multidisciplinary Research, University of Belgrade, Institute of Nuclear Sciences VINCA, P.O. Box 522, 11001 Belgrade, Serbia; 3Department of Molecular Pathology, The Institute for Molecular Medicine, Huntington Beach, CA 92647 USA Corresponding author: Garth L Nicolson, PhD, MD (H), Department of Molecular Pathology, The Institute for Molecular Medicine, Huntington Beach, CA 92647 USA Submission Date: October 3, 2016, Accepted Date: December 18, 2016, Publication Date: December 30, 2016 Citation: Veljkovic V.V., Glisic S., Perovic V., Veljkovic N., Nicolson G.L.. Pomegranate (Punica granatum): a natural source for the development of therapeutic compositions of food supplements with anticancer activities based on electron acceptor molecular characteristics. Functional Foods in Health and Disease 2016; 6(12):769-787 ABSTRACT Background: Numerous in vitro and in vivo studies, in addition to clinical data, demonstrate that pomegranate juice can prevent or slow-down the progression of some types of cancers. Despite the well-documented effect of pomegranate ingredients on neoplastic changes, the molecular mechanism(s) underlying this phenomenon remains elusive. Methods: For the study of pomegranate ingredients the electron-ion interaction potential (EIIP) and the average quasi valence number (AQVN) were used. These molecular descriptors can be used to describe the long-range intermolecular interactions in biological systems and can identify substances with strong electron-acceptor properties.
    [Show full text]
  • Antioxidant Activity of Pomegranate Juice and Its Relationship with Phenolic Composition and Processing
    J. Agric. Food Chem. 2000, 48, 4581−4589 4581 Antioxidant Activity of Pomegranate Juice and Its Relationship with Phenolic Composition and Processing Marı´a I. Gil,† Francisco A. Toma´s-Barbera´n,† Betty Hess-Pierce,‡ Deirdre M. Holcroft,§ and Adel A. Kader*,‡ Department of Pomology, University of California, Davis, California 95616, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 4195, Murcia 30080, Spain, and Department of Horticultural Science, Private Bag X1, Matieland 7602, South Africa The antioxidant activity of pomegranate juices was evaluated by four different methods (ABTS, DPPH, DMPD, and FRAP) and compared to those of red wine and a green tea infusion. Commercial pomegranate juices showed an antioxidant activity (18-20 TEAC) three times higher than those of red wine and green tea (6-8 TEAC). The activity was higher in commercial juices extracted from whole pomegranates than in experimental juices obtained from the arils only (12-14 TEAC). HPLC- DAD and HPLC-MS analyses of the juices revealed that commercial juices contained the pomegranate tannin punicalagin (1500-1900 mg/L) while only traces of this compound were detected in the experimental juice obtained from arils in the laboratory. This shows that pomegranate industrial processing extracts some of the hydrolyzable tannins present in the fruit rind. This could account for the higher antioxidant activity of commercial juices compared to the experimental ones. In addition, anthocyanins, ellagic acid derivatives, and hydrolyzable tannins were detected and quantified in the pomegranate juices. Keywords: Pomegranate; Punica granatum; Punicaceae; juice; phenolics; anthocyanins; ellagic acid; punicalagin; tannins; antioxidant activity; ABTS; DPPH; DMPD; FRAP INTRODUCTION 1986b), leaf (Tanaka et al., 1985; Nawwar et al., 1994b), and the fruit husk (Mayer et al., 1977) are very rich in Epidemiological studies show that consumption of ellagitannins and gallotannins.
    [Show full text]
  • Pomegranate's Ellagitannins: Metabolism and Mechanisms Of
    Mini review Nutri Food Sci Int J Volume 9 Issue 4 - October 2019 Copyright © All rights are reserved by Paolo Silacci DOI: 10.19080/NFSIJ.2019.09.555766 Pomegranate’s Ellagitannins: Metabolism and Mechanisms of Health Promoting Properties Paolo Silacci* and Marco Tretola Agroscope, 1725 Posieux, Switzerland Submission: October 21, 2019; Published: October 30, 2019 *Corresponding author: Paolo Silacci, Animal Biology Research group, Agroscope, 1725 Posieux, Switzerland Abstract The health benefits of pomegranate consumption have been known since the antiquity. More recently, the biological activities of pomegranate, including its anti-bacterial, antioxidant, anti-inflammatory and anticarcinogenic properties, have raised great interests in the scientific community and they have been the subject of a large number of scientific studies. Several phenolic compounds are present in various parts of the fruit, of which ellagitannins, hydrolysable tannins considered responsible for most health benefits of pomegranate. Despite all these efforts, the actual effectors remain unidentified and the mechanisms of action only partially elucidated. This is probably due to the complex catabolic pathway of these molecules that takes place in the gastrointestinal tract, involving both microbial and cellular modifications before entering the bloodstream. The diversity of the intestinal microbiota in the human population leads to the production of different metabolites in individuals, contributing to the heterogeneity of the response to dietary pomegranate assumption.
    [Show full text]
  • Compositions and Methods for Improving
    (19) TZZ¥ ZZ_T (11) EP 3 278 800 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: A61K 31/352 (2006.01) A61K 36/00 (2006.01) 10.04.2019 Bulletin 2019/15 A61K 36/185 (2006.01) (21) Application number: 17186188.3 (22) Date of filing: 23.12.2011 (54) COMPOSITIONS AND METHODS FOR IMPROVING MITOCHONDRIAL FUNCTION AND TREATING MUSCLE-RELATED PATHOLOGICAL CONDITIONS ZUSAMMENSETZUNGEN UND VERFAHREN ZUR VERBESSERUNG DER MITOCHONDRIENFUNKTION UND BEHANDLUNG VON PATHOLOGISCHEN MUSKULÄREN ERKRANKUNGEN COMPOSITIONS ET PROCÉDÉS POUR AMÉLIORER LA FONCTION MITOCHONDRIALE ET TRAITER DES CONDITIONS PATHOLOGIQUES MUSCULAIRES (84) Designated Contracting States: • PIRINEN, Eija AL AT BE BG CH CY CZ DE DK EE ES FI FR GB 00240 Helsinki (FI) GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO •THOMAS,Charles PL PT RO RS SE SI SK SM TR 21000 Dijon (FR) • HOUTKOOPER, Richardus (30) Priority: 23.12.2010 US 201061426957 P Weesp (NL) • BLANCO-BOSE, William (43) Date of publication of application: CH-1090 La Croix (Lutry) (CH) 07.02.2018 Bulletin 2018/06 • MOUCHIROUD, Laurent 1110 Morges (CH) (60) Divisional application: • GENOUX, David 18166896.3 / 3 372 228 CH-1000 Lausanne (CH) 18166897.1 / 3 369 420 (74) Representative: Abel & Imray (62) Document number(s) of the earlier application(s) in Westpoint Building accordance with Art. 76 EPC: James Street West 11808119.9 / 2 654 461 Bath BA1 2DA (GB) (73) Proprietor: Amazentis SA (56) References cited: 1015 Lausanne (CH) US-A1- 2003 078 212 US-A1- 2009 326 057 (72) Inventors: • JUSTIN R.
    [Show full text]
  • Pomegranate: Constituents, Bioactivities and Pharmacokinetics
    ® Fruit, Vegetable and Cereal Science and Biotechnology ©2010 Global Science Books Pomegranate: Constituents, Bioactivities and Pharmacokinetics Rufeng Wang1,2 • Yi Ding2 • Ruining Liu3 • Lan Xiang4 • Lijun Du2* 1 School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100102, China 2 Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China 3 Department of Pharmacy, First Teaching Hospital of Tsinghua University, Beijing 100016, China 4 School of Pharmacy, Shandong University, Jinan 250012, China Corresponding author : * [email protected] ABSTRACT Pomegranate (Punica granatum L.), a species of Punicaceae, has recently become of great interest to the scientists who engage themselves in pharmaceutical, nutriological and pharmacological research, and new drug development, due to its distinctive multiple officinal parts and multiple bioactivities such as hypolipidemic, antioxidant, antiviral, anti-neoplastic, antibacterial, anti-diabetic, anti- diarrheal, and helminthic effects. In the present review, we reported the research on pomegranate as to chemical constituents, bioactivities and pharmacokinetics in combination with the recent publications. The constituents of this plant were thoroughly reviewed, and the biosynthetic pathways of major compounds were discussed based on our research results and those reported in the literatures. The bioactivities categorized by vascular protection, digestive protection, anti-pathogenic microbes, anticancer,
    [Show full text]
  • Solvent Influence on the Antioxidant Activity of Pomegranate Extracts Corina Iuliana Costescu 1, Daniela Rus 1, Nicoleta G
    Available online at http://journal-of-agroalimentary.ro Journal of Agroalimentary Processes and Journal of Agroalimentary Processes and Technologies Technologies 2015, 21(1), 21-27 Solvent influence on the antioxidant activity of pomegranate extracts Corina Iuliana Costescu 1, Daniela Rus 1, Nicoleta G. Hădărugă 1, Veronica Pogor 1, Mădălina Badea 1, Evelyn Mateican 1, Raymond Szakal 1, Petru Olaru 1, Daniel Ioan Hădărugă 2* 1Department of Food Science, Banat's University of Agricultural Sciences and Veterinary Medicine “King Michael Iof Romania”-Timisoara, Calea Aradului 119, 300645 – Timisoara, Romania 2Department of Applied Chemistry, Organic and Natural Compounds Engineering, Polytechnic University of Timisoara, Carol Telbisz 6, 300001-Timisoara, Romania Received: 16 Novmber 2014; Accepted: 15 December 2014 ____________________________________________________________________________________ Abstract In this study we tried to assess the antioxidant activity of different parts of pomegranate fruit extracts (Punica granatum ). In order to determine the antioxidant activity it was used the 2,2-diphenyl-1-picryl- hydrazyl method and were studied seeds or whole fruits of pomegranate, respectively crushed seeds. The solvent used for the extraction was ethanol-water solution of various concentrations, including distilled water . Keywords : pomegranate fruit (Punica granatum ), antioxidant activity, DPPH ______________________________________________________________________________________ 1. Introduction Pomegranate ( Punica granatum ), is a fruit that From the chemical point of view, pomegranate seeds grows on small trees and it’s widely used in contain C and K vitamins, as well as polyphenols various food products, juices and alcoholic (such as ellagitanins) and flavonoids, which are an beverages. It has it’s origins in Iran and is important source of fiber and micronutrients. It also cultivated since antiquity.
    [Show full text]
  • 2018032952430781.Pdf
    The Journal of the Science of Food and Agriculture, 2018,2(3), 71-83 http://www.hillpublisher.com/journals/jsfa Review of the Phytochemical, Pharmacological and Toxicological Properties of Punica granatum L., (Lythraceae) Plant Anna Muriel Talavera Jacinto College of Pharmacy / University of Perpetual Help System DALTA Las Piñas City Campus Alabang – Zapote Road, Pamplona 3, Las Piñas City, Metro Manila 1740, Philippines How to cite this paper: Jacinto, A.M.T. Abstract (2018) Review of the Phytochemical, Pharmacological and Toxicological Pomegranate is considered as “wonder fruit” because of its various therapeutic effects. Properties of Punica granatum L., This paper aims to provide an up-to-date overview of the phytochemical, pharmaco- (Lythraceae) Plant. The Journal of the logical and toxicological properties. The research strategy includes review of the exist- Science of Food and Agriculture, 2(3), ing literature in the ISI Web of Knowledge (Thomson Reuters), textbooks and Phar- 71-83. macopeias from year 1978 to 2016. Phytochemical screening shows that its fruit, juice, http://dx.doi.org/10.26855/jsfa.2018.03. 004 seeds, flowers, leaves, root, bark and fruit peel contains the following; gallic acid, ellagic acid, punicalin, punicalagin, caffeic acid, citric acid, malic acid, succinic acid, Corresponding author: Anna Muriel tartaric acid, acetic acid, oxalic acid, shikimik acid, maleic acid, furamic acid Talavera Jacinto, College of ellagitannins, pelletierine alkaloids, piperidine alkaloid, isopelletierine, me- Pharmacy / University
    [Show full text]
  • Pomegranate: a Promising Avenue Against the Most Common Chronic Diseases and Their Associated Risk Factors (Review)
    INTERNATIONAL JOURNAL OF FUNCTIONAL NUTRITION 2: 6, 2021 Pomegranate: A promising avenue against the most common chronic diseases and their associated risk factors (Review) ALESSANDRO LAVORO1, LUCA FALZONE2, GIUSEPPE GATTUSO1, ROSSELLA SALEMI1, GIOVANNI CULTRERA1, GIAN MARCO LEONE1, GIUSEPPA SCANDURRA3, SAVERIO CANDIDO1,4 and MASSIMO LIBRA1,4 1Department of Biomedical and Biotechnological Sciences, University of Catania, I‑95123 Catania; 2Epidemiology and Biostatistics Unit, National Cancer Institute ‑ IRCCS ‘Fondazione G. Pascale’, I‑80131 Naples; 3Medical Oncology Unit, Cannizzaro Hospital, I‑95126 Catania; 4Research Center for the Prevention, Diagnosis and Treatment of Tumors, University of Catania, I‑95123 Catania, Italy Received March 6, 2021; Accepted April 20, 2021 DOI: 10.3892/ijfn.2021.16 Abstract. Pomegranate is an important source of several 6. Protective role of pomegranate in cardiovascular diseases bioactive compounds with beneficial properties. As widely 7. Prebiotic properties described in the literature, pomegranate can be considered as 8. Anticancer effects a functional food able to promote the maintenance of a good 9. Conclusions health status. The present review article summarizes the prop‑ erties of pomegranate, such as antioxidant, anti‑inflammatory, anti‑aging, prebiotic and anticancer effects, as well as its 1. Introduction protective role against metabolic disorders and cardiovascular diseases. Pomegranate and its phytocompounds can prevent Pomegranate, botanical name Punica granatum L., is a largely the development of several chronic diseases. Additionally, consumed fruit deriving from the deciduous tree of Punica L. pomegranate can be considered an adjunct for current genus, Punicaceae family (1). This fruit inherits its name from therapeutic strategies able to improve the standard treatment the Latin words ‘pomus’ and ‘granum’ (apple with grains), which efficacy and reduce side‑effects.
    [Show full text]