(12) United States Patent (10) Patent No.: US 7,919,636 B2 Seeram Et Al
USOO7919636B2 (12) United States Patent (10) Patent No.: US 7,919,636 B2 Seeram et al. (45) Date of Patent: Apr. 5, 2011 (54) PURIFICATIONS OF POMEGRANATE Aviram, M., et al., “Pomegranate juice consumption inhibits serum ELLAGTANNINS AND THEIR USES angiotensin converting enzyme activity and reduces systolic blood THEREOF pressure.” (2001) Atherosclerosis, 158: 195-198. Cerda, B., et al., “Evaluation of bioavailability and metabolism in the (75) Inventors: Navindra P. Seeram, Los Angeles, CA rat of punicalagin, an antioxidant polyphenol from pomegranate juice.” (2003) Eur, J. Nutr., 42:18-28. (US); David Heber, Los Angeles, CA Cerda, B., et al., “Repeated oral administration of high doses of the (US) pomegranate elagitannin punicalaginto rats for 37 days is not toxic.” (2003) J. Agric. Food Chem. 51:3493-3501. (73) Assignee: The Regents of the University of Doig, A., et al., “Isolation and structure elucidation of punicalagin, a California, Oakland, CA (US) toxic hydrolysable tannin, from Terminalia oblongata.” (1990) J. Chem. Soc. Perkin Trans. I, 2317-2321. (*) Notice: Subject to any disclaimer, the term of this El-Toumy, S., et al., “Two ellagitannins from Punica granatum patent is extended or adjusted under 35 heartwood.” (2002) Phytochemistry, 61:971-974. U.S.C. 154(b) by 248 days. Filippich, L., et al., “Hepatotoxic and nephrotoxic principles in Terminalia oblongata.” (1991) Research in Veterinary Science, (21) Appl. No.: 12/143,657 50:17O-177. Gil, M., et al., “Antioxidant activity of pomegranate juice and its (22) Filed: Jun. 20, 2008 relationship with phenolic composition and processing.” (2000) J. Agric. Food Chem., 48:4581-4589.
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