m

Product Specifications m 4 mm (Analytical + Guard) quadruple-pulse waveform 8. 8. Verbascose 19. 4 mm (Analytical + Guard) quadruple-pulse waveform

8. 8. Galactose Verbascose 19.

20 20 Column: Column: µ Dionex CarboPac PA210-Fast-4 Eluent: 500 KOH cartridge Dionex EGC Eluent Source: Flow Rate: 12 mM KOH Volume: Inj. 0.8 mL/min 10 µL 0.002" Gasket: Temperature: 30 ˚C Detection: Integrated Amperometry Electrodes: Working: Reference: Au (-Disposable) Ag/AgCl Standard 5 ppm (mg/L) Concentration: Peaks: Xylitol 1. 12. Sorbitol 2. Perseitol 3. 13. 4. Glucoheptose 14. Maltitol 5. 15. 6. 17. Raffinose 16. 7. Gentibiose 18. 9. 10. 20. 11. 20 Eluent: Dionex EGC 500 KOH cartridge Eluent Source: Flow Rate: 12 mM KOH Volume: Inj. 0.8 mL/min 10 µL 0.002" Gasket: Temperature: 30 ˚C Detection: Integrated Amperometry Electrodes: Working: Reference: Au (Carbohydrate-Disposable) Ag/AgCl Standard 5 ppm (mg/L) Concentration: Peaks: Xylitol 1. Xylose 12. Sorbitol 2. Perseitol 3. Ribose 13. Fucose 4. Glucoheptose 14. Maltitol 5. Lactose 15. Rhamnose 6. Stachyose 17. Raffinose 16. Arabinose 7. Gentibiose 18. Glucose 9. Sucrose 10. Cellobiose 20. Fructose 11. Column: Column: µ Dionex CarboPac PA210-Fast-4 20 19 19 18 15 Format: 20 sugars 17 min—Whammogram Format: 20 sugars 17 min—Whammogram and verbascose are not digested human by gastrointestinal enzymes the small in intestine, and are poorly absorbed most by people. As such, they are referred to as prebiotic . Interest in raffinoseoligosaccharides and (RFOs) family has increased during the last decade due to selectively to abilities health-promoting their stimulate beneficial bacteria. Aside from their prebiotic characteristics, fructans and RFOs are also emergingas important immune stimulators animals in and humans. 18 15 Format: 20 sugars 17 min—Whammogram Format: 20 sugars 17 17 17 4 × 150 16 4 × 150 15 16 m 10 µ 15 m Minutes 10 µ Minutes 14 14 13 12 13 11 10 12 11 9 10 5 8 9 5 7 8 6 7 5 6 5 4

3 4 2 3 1 2 1 4 PA210-Fast- CarboPac

0 4 PA210-Fast- CarboPac

0 0 60 nC 1 0 60 nC 1 Increased interest in small Numerous oligosaccharides, consisting of 3–5 units, occur in foods. These oligosaccharides generally are The nondigestible. considered (RFOs) oligosaccharides include family stachyose ), (a raffinose (a verbascose and ), (a pentasaccharide). They are found in vegetables, such as peas, beans, and lentils. Unlike other sugars, raffinose, stachyose, .

™ CarboPac ™ Dionex ™

™

CarboPac ™ of this packing allows eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes. fast, high-resolution separations for most most for separations fast, high-resolution mono- through pentasaccharides a variety in of applications includingfood and beverage analyses. The columns are packed with polymeric, microporous hydrophobic, a anion exchange resin stable over entire range The of pH unique pH-stability 0–14. food matrices The Thermo Scientific PA210-Fast-4µm (2 columns and mm 4 mm) are the latest additions to the Dionex CarboPac separations carbohydrate for columns of family The new column was developed to provide Predictable, high-resolution separations of simple sugars in Column Thermo ScientificThermo PA210-Fast-4µm CarboPac Dionex Dionex ™ CHROMATOGRAPHY rhamnose, stachyose, raffinose, and raffinose, stachyose, rhamnose, verbascose in food and beverage samples in ≤ 20 minutes efficiency separations of food sugars separations efficiency sucrose, fructose, galactose, Resolve and quantify glucose, Resolve and quantify glucose, Predictable, high-resolution, high high-resolution, Predictable, • • of food samples. pentasaccharides in various types in various pentasaccharides columns for high resolution analysis analysis columns for high resolution and tetra- tri-, di-, of mono-, Thermo Scientific Column Test Chromatogram: CarboPac PA210-Fast-4 m 2 mm –Figure 1 2 Isocratic separation of mono and Column: See Chromatogram (2 mm) standards (the 5 most Eluent: 12 mM KOH AnalyticalAnalytical Column Column Source: Dionex EGC KOH Cartridge common sugars of interest) Temperature: 30 °C Flow Rate: 0.20 mL/min The Dionex CarboPac PA210-Fast-4µm Inj Volume: 2.5 µL

column has been designed to provide nC Gasket: 0.002" 5 Detection: Integrated Amperometry fast separations of common mono- and quadruple-pulse waveform Electrodes: , and also oligosaccharides up Working: Au (Carbohydrate-Disposable) to five units long. Using 12 mM KOH, Reference: Ag/AgCl Standard The five sugars can be separated within Concentration: 5 ppm Analytical Guard Column 8 min on the 150 mm long columns on both Analytical Guard Column Peaks: 1. Fucose 2. Arabinose the 2 mm ID and 4 mm ID column formats 3. Glucose 4. Sucrose (Figure 1). nC 5 5. Fructose Analysis of Sake Sake is a product prepared by microbial fermentation of polished rice grains. Eighteen mono- and di-saccharides are Minutes reported to be present in Sake. A standard Figure 1. Separation of a five-carbohydrate standard using the Dionex CarboPac with known concentrations of these PA210-Fast-4µm, 2 mm column. 18 sugars was prepared. The bottom panel Sake Glycans on CarboPac PA210-Fast-4 m 4mm of Figure 2 shows the separation of the Standards, Sweet Cooking Sake – Figure 3

standards. The top panel of Figure 2 shows Column: Dionex CarboPac A210-Fast-4µm 4 mm (Analytical + Guard) the separation of a 1000-fold diluted Sake 5 Sweet Cooking Sake Eluent: 14 mM KOH sample. The separation reveals significant Source: Dionex EGC KOH Cartridge Temperature: 30 °C amounts of glucose, sucrose, fructose, and Flow Rate: 0.80 mL/min nC Inj Volume: 10 µL , and smaller amounts of kojibiose, Samples: A. Standards gentibiose, cellobiose, and sakebiose. B. Sweet Cooking Sake Gasket: 0.002" Detection: Integrated Amperometry quadruple-pulse waveform Electrodes: Working: Au (Carbohydrate-Disposable) Reference: Ag/AgCl 5 5 Standards Standard Peaks: Name Concentration (ppm): 1. Fucose 2 2. 3 3. Rhamnose 2 nC 4. Arabinose 2 5. Galactose 2 5 6. Glucose 2 7. 2 8. Sucrose 5 9. Fructose 5 10. Ribose 3 11. Lactose 4 Minutes 12. 5 13. Raffinose 6 14. Kojibiose 2 15. Gentibiose 10 16. Cellobiose 14 17. Sakebiose 3 18. Maltose 15

Figure 2. Separation of Sake carbohydrates using the Dionex CarboPac PA210-Fast-4µm, 4 mm column. Product Specifications

m 088955 088956 088953 088954

Part Number

 m Fast 4mm m Fast

Maltose 13. 15 Nigerose 12. 3 1-Kestose 11. 2 Gentibiose 10. 8 9. 7 Kojibiose 8. 1 Sucrose 7. 5 Fructose 6. 3 Glucose 5. 2 Rhamnose 4. 2 Fucose 3. 2 Trehalose 2. 2 Fucitol 1. 1 Peaks: Peaks: Name Concentration (ppm): Standard Reference: Reference: Ag/AgCl Working: Working: Au (Carbohydrate-Disposable) Electrodes: quadruple-pulse waveform Detection: Detection: Integrated Amperometry Gasket: 0.002" Gasket: Samples: Samples: Chromatogram See Inj Volume: Inj Volume: 10 µL Flow Rate: Flow Rate: 0.80 mL/min Temperature: Temperature: 30 °C Source: Source: Dionex EGC KOH Cartridge Column: Column: µ PA210-Fast-4 Dionex CarboPac Eluent: 48 mM KOH Guard) 4 mm (Analytical + Thermo Fisher Scientific, CA USA is Sunnyvale, ISO 9001 Certified. +41 61 716 77 00 +43 1 333 50 34 0 +44 1442 233555

. +65 6289 1190 +46 8 556 468 00 +886 2 8751 6655 Honey Standards +1 800 532 4752 Cloer Honey New Zealand dilution dilution dilution Manuka Honey Russia/CIS Singapore Sweden Switzerland Taiwan UK/Ireland USA Unprocessed Honey Unprocessed Honey   SPECIFICATIONS

2 mm/4 mm × 150 mm × 150 mm 2 mm/4 (55% cross-linking) agglomerated with 43 MicroBead nm 6% quaternarycross-linked latex amine-functionalized 4.0 μm diameter ethylvinylbenzene/divinylbenzene substrate ethylvinylbenzene/divinylbenzene μm diameter 4.0 66 μeq/column 80% compatible withpH 0-14, common HPLC Solvents 60 °C 4,000 psi at 0.2 mL/0.8 mL per minute (guard and analytical) 30 °C 0.2 mL/min 0.8 mL/min 2 mm; 4 mm Potassium or Sodium Hydroxide

. +1 561 688 8700 +64 9 980 6700 +31 76 579 55 55 Minutes +43 1 333 50 34 0

+46 8 556 468 00 +81 6 6885 1213 +82 2 3420 8600 Korea Latin America Middle East Netherlands New Zealand Norway Japan

5 Standards, and three different Honeys – Figure 4 Figure – Honeys different and three Standards,



. Comparison of Honey Glycans on CarboPac PA210-4 on CarboPac Glycans of Honey Comparison nC 4 mm column. Figure 3. Comparison of honey carbohydrates on a Dionex CarboPac PA210-Fast-4µm, Dionex CarboPac PA210-Fast-4µm Analytical Column mm) (2 × 150 Dionex CarboPac PA210-Fast-4µm Guard Column × 30 (4 mm) Dionex CarboPac PA210-Fast-4µm Guard Column (2 × 30 mm) Recommended Operating Temperature: Recommended Flow Rate: Ionic Forms Eluents: Ordering Information For more information contact or to place an order, the Thermo Scientific Dionex Products office nearest you or your local distributor. Phone numbers and addresses for worldwide subsidiaries can be found the in About Us section of www.thermoscientific.com. Description Dionex CarboPac PA210-Fast-4µm Analytical mm) Column × 150 (4 Resin Composition: Anion Exchange Capacity: Chemical Compatibility: Limit:Temperature OperatingTypical Conditions: 5 +43 1 333 50 34 0

+45 70 23 62 60 +49 6103 408 1014 +358 10 3292 200 +33 1 60 92 48 00 +91 22 6742 9494 +39 02 950 591 Europe-Other Finland France Germany India Italy Denmark

(free call domestic) +61 3 9757 4300 +32 53 73 42 41 +1 800 530 8447 +43 1 333 50 34 0 +43 810 282 206 800 810 5118 400 650 5118

+55 11 2730 3006 

Dionex CarboPacPA210-Fast-4µm column delivers reproducible performance. is carefully controlled to ensure that every (resin synthesis, amination, and packing and testingof the chromatographic columns) structure ensures long column life and easy cleanup. The entire manufacturing process the entire pH range. Its highly cross-linked CarboPac PA210-Fast-4µm columns offers selectivity stability and over exceptional The unique pellicular resin of the Dionex determining the source of the honey. Performance Guaranteed is a little different which can be valuable in The carbohydrate profiles of each of the honeys maltose, nigerose and kojibiose are present concentrations. lower at manuka honey containglucose and fructose as the most abundant sugars, and sucrose, clover honey, beekeeper’s honey, and beekeeper’s theclover honey, honey, All the honey types namely supermarket in waterin and compared against a known standard sugars. of 13 All the honey types were diluted 1000-fold analysed along with a known a standard in known standard in a a with along analysed Figure 3. local beekeeper and from New Zealand were from the source flowersby thehive. Honey preparations from a local supermarket, a carbohydrates found the in nectarretrieved Analysis of Honey Honeyis natural product, and tends to contain PS71994-EN 0316S Canada China Austria Belgium Brazil Africa Australia manners that might infringe the intellectual property rights of others. Specifications,Not terms all products and pricing are are available subject in allto change. countries. Please consult your local sales representative for details. ©2016 Thermo Fisher Scientific Inc. All rights reserved. ISO is a trademark of the InternationalAll other trademarksStandards areOrganization. the property of Thermo Fisher Scientific and its subsidiaries.example This ofinformation the capabilities is presented of Thermo as an Fisher Scientific products. It is not intended to encourage use of these products in any www.thermofisher.com/chromatography