US 2016O295860A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2016/0295860 A1 DAGHER et al. (43) Pub. Date: Oct. 13, 2016

(54) A NOVEL COMPOSITION AND METHOD OF AOIN 25/00 (2006.01) USE TO CONTROL PATHOGENS AND AOIN 31/02 (2006.01) PREVENT DISEASES IN SEEDS (52) U.S. Cl. CPC ...... A0IN 37/16 (2013.01); A0IN 25/00 (71) Applicant: AGRI-NEO INC., Toronto (CA) (2013.01): A0IN 31/02 (2013.01); A23B 9/26 (2013.01); A23B 9/30 (2013.01); A23V (72) Inventors: Fadi DAGHER, Laval (CA); Nicholas 2002/00 (2013.01) DILLON, Toronto (CA); Kenneth Sherman UNGAR, Scarborough (CA) (57) ABSTRACT (73) Assignee: AGRI-NEO INC., Toronto, ON (CA) The invention relates to a composition of water-soluble ingredients (CWSI) which when solubilized in water (W) (21) Appl. No.: 15/038,211 and either in the presence of a wetting agent and/or in the presence of at least one agriculturally acceptable solvent, (22) PCT Fed: Nov. 13, 2014 forms a synergistic composition useful for the control of (86) PCT No.: PCT/CA2O14/051088 pathogens and/or the prevention of diseases associated with the presence of said pathogens in and/or on seeds. Said S 371 (c)(1), composition of water-soluble ingredients (CWSI) comprises (2) Date: May 20, 2016 at least one oxidizer in liquid form or solid form, or a precursor thereof in liquid or solid form, said at least one Related U.S. Application Data agriculturally acceptable solvent is soluble in water (W); the (60) Provisional application No. 61/907,560, filed on Nov. water-soluble ingredients (CWSI) is dissolved in the water 22, 2013. (W), in a weight ratio (CWSI)/(W) ranging from 1:100 to 1:4, and when present, said at least one agriculturally Publication Classification acceptable solvent represents from 2% by volume to 70% by Volume of the total Volume of the Synergistic composition to (51) Int. C. be formed. The invention also relates to a synergistic com AOIN 37/16 (2006.01) position for the control of pathogens and/or the prevention A23B 9/30 (2006.01) of diseases associated with the presence of said pathogens in A23B 9/26 (2006.01) and/or on seeds, and a method and a use of the same. Patent Application Publication Oct. 13, 2016 Sheet 1 of 3 US 2016/029586.0 A1

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A NOVEL COMPOSITION AND METHOD OF ent application No. 2,569,025; International PCT patent USE TO CONTROL PATHOGENS AND application WO95/02330 and EP patent application No. 0 PREVENT DISEASES IN SEEDS 648 418). 0005 Also, peracetic acid (also known under the trade FIELD OF THE INVENTION name peracid) is a strong oxidizing agent which is known for having virucidal, bactericidal, fungicidal and algaecidal 0001. The invention relates to the field of agriculture, properties. Peracetic acid was patented in 1950 for the food safety and post-harvest food treatments to address the treatment of raw plant tissue, especially for the treatment of lack of useful methods to prevent bacterial contamination of fruits and vegetables, to reduce spoilage from bacteria and dry raw agricultural commodities. The novel composition fungi destined for processing (U.S. Pat. No. 2,512.640). provides unexpectedly high degree of control required for Nowadays, peracetic acid is commonly use in food process food safety. The invention also relates to a method of use ing and handling as a sanitizer for food contact surfaces and thereof. as a disinfectant for fruits, vegetables, meat and eggs (NOSB TAP Materials database compiled by OMRI, Nov. 3, 2000, 0002 More particularly, the invention relates to a com 7 pages). In the production of fruits and vegetables, per position of water-soluble ingredients, said water-soluble acetic aqueous Solutions have been Suggested to control ingredients comprising at least one oxidizer, at least one pathogenic organisms on growing plants (U.S. Pat. No. lower alcohol and optionally at least one wetting agent, 6,024,986; U.S. Pat. No. 6,165,483; and U.S. Pat. No. which when solubilized in a water-containing solvent forms 6,238,685). an aqueous, Synergistic composition useful for the control of 0006. As per Applicant’s published international patent pathogens and/or the prevention of diseases associated with application WO 2012/051699, a solution of peracetic acid the presence of said pathogens in and/or on seeds, more generated in situ in combination with a plant defence preferably edible seeds such as hemp seeds, flax seeds or enhancer demonstrated excellent anti-bactericidal and anti chia seeds. Also, the invention relates to an aqueous, Syn fungal properties. More particularly, in this international ergistic composition obtained by dissolving the above-men patent application it was evidenced a synergy of peracetic tioned composition in water, and relates to method and use acid and at least one plant defence enhancer for the control of said aqueous, Synergistic composition for the control of of pathogens in and onto growing plants. Furthermore, pathogens and/or the prevention of diseases associated with according to a particularly preferred embodiment of said the presence of said pathogens in and/or on seeds, more published international patent application WO2012/051699, preferably edible seeds such as hemp seeds, flax seeds or the presence of a Surfactant in a synergistic mix of a chia seeds. precursor of peracetic acid and potassium silicate (i.e. a SAR inducer), enhances the release of peracetic acid when BACKGROUND OF THE INVENTION admixed with water. 0007. However, concerning seeds, more particularly 0003 Published international application No. WO2007/ seeds having external shells, especially edible seeds Such as O92180 describes a fertilizer composition for application to hemp seeds, flax seeds and chia seeds, the person skilled in a seed, plant, growth medium or growth solution, said the art notes that existing compositions do not allow an composition comprising an oxidizing agent wherein bio oxidizer to cover the surface of the seeds efficiently in order available oxygen is released upon contact to the composition to kill pathogens present thereon and/or therein. with water, optionally a solvent Such as methyl ketone, 0008. Therefore, there was still a strong need for a methyl isobutyl ketone, cyclohexanonon, Xylenes, toluene, composition allowing to obtain an efficient control and/or chlorobenzene, paraffins, kerosene, white oil, alcohols, treatment of diseases associated with seeds, especially con methylnaphthalene, trimethylbenzene, trichloroethylene, cerning seeds having external shells, especially edible grains N-methyl-2-pyrrolidone and tetrahydrofurfuryl alcohol and seeds Such as hemp seeds. Also, there was a strong need (THFA), and eventually other additives selected from the for method and use allowing to control and/or treat effi group consisting of companion cations, cation reducing ciently seeds having pathogens present thereon. agents, pH modulators, nutrients, organic compounds, pen 0009. Also, it is to be noted that literature did not etrants, microorganisms, pesticides, fungicides, insecticides, recommend using alcohol on seeds because the person nematocides, herbicides, water trapping agents, enzymes, skilled in the art knows that alcohol at low dosage does not Surfactants, wetting agents, spreaders, stickers and growth kill bacteria. In this regard, reference can be made to the hormones. This published international application content of the article of Mena et al., “Influence of Ethanol on WO2007/092180 further relates to a method of use of said Probiotic and Culture Bacteria Lactobacillus bulgaricus and fertilizer composition. However, this published international Streptococcus thermophilus within a Therapeutic Product’. application does not provide a novel Solution to control the Open Journal of Medical Microbiology, 2012, 2, 70-76. In high level of pathogens on edible food commodities. this article Mena et al. showed that ethanol added to yogurt 0004 Peracetic acid (CHO) in an aqueous solution is does not affect the count of Lactobacillus sp. a mixture which is further comprising acetic acid 0010 Also, it is to be noted in addition to the teaching of (CHCOOH) and hydrogen peroxide (HO). Typically, the above-mentioned article of Mena et al. that not only peracetic acid (also identified hereinafter under the acronym alcohol does not kill bacteria at low levels but also it is used PAA) is produced by reacting acetic acid and hydrogen (at concentration of around 1 to 5%) as a carbon source for peroxide. It is also well known to generate a liquid Solution bacteria to promote their growth. In this regard, reference comprising PAA starting from the dissolution of a powdered can be made to the content of the article of Smith et al., mixture (U.S. Pat. No. 7,291.276; UK patent application No. “Microbial Synergia via an Ethanol-Triggered Pathway'. 2.355, 198; FR patent application 2,728,171; Canadian pat Molecular and Cellular Biology, May 2004, p. 3874-3884. US 2016/0295860 A1 Oct. 13, 2016

0011 Surprisingly, the Applicant has recently discovered 0027. Also, it is worth mentioning that said synergistic that when using at least one oxidizer in combination with at composition, method and use is particularly efficient to kill least one agriculturally acceptable solvent and/or wetting human pathogens such as bacteria (e.g. E. coli, Salmonella agent, soluble in water, and much more preferably at least spp., Listeria monocytogenes), coliforms that may be pres one alcohol, optionally with or without at least one wetting ent on the Surface of said seeds, as well as yeast and mould agent (e.g. a surfactant), it is possible to obtain a synergy which may be present on the Surface of said seeds. between above ingredients to provide an aqueous, Synergis tic composition, a method and a use allowing to cover the SUMMARY OF THE INVENTION seeds surfaces efficiently with said at least one oxidizer in order and provide an efficient control and/or treatment of 0028. According to an embodiment, the invention relates seeds having pathogens present thereon. to a first composition of water-soluble ingredients (CWSI) 0012. Furthermore, it is worth mentioning that the Appli which when solubilized in water (W) and either in the cant has surprisingly discovered that the presence of a presence of a wetting agent, or preferably in the presence of wetting agent along with an oxidizer in an aqueous Solution at least one agriculturally acceptable solvent, or more pref of the same: erably in the presence of a wetting agent and at least one 0013 allows a better coverage of the seeds. agriculturally acceptable solvent, forms a synergistic com 0014 Furthermore, it is worth mentioning that the Appli position useful for the control of pathogens and/or the cant has surprisingly discovered that the presence of alcohol prevention of diseases associated with the presence of said along with an oxidizer in an aqueous Solution of the same: pathogens in and/or on seeds, preferably seeds having exter 0015 allows a better coverage of the seeds: nal shells, more preferably edible seeds and much more 0016 helps drying the seeds after having applied the preferably hemp, flax and chia seeds, wherein (i) said aqueous solution; composition of water-soluble ingredients (CWSI) comprises 0017 prevents the release of mucilage from seeds such at least one oxidizer in liquid form or solid form, or a as flax and chia, and thus maintains the nutritional precursor thereof in liquid or solid form, and preferably value and natural composition of the seeds intact; 0029 liquidqu1d ppreformed pperacetic acid and/or in-situ and/or generated peracetic acid from powder precursors; and/ 0018 maintains organoleptic properties of said seeds. O 0019. Furthermore, it is worth mentioning that the Appli 0030 liquid hydrogen peroxide and/or hydrogen per cant has surprisingly discovered that the presence of alcohol oxide released from a powder persalt, and/or and a wetting agent, along with an oxidizer in an aqueous 0.031 other liquid oxidizers and/or powder oxidizers Solution of the same: Such as those generating iodine, chlorine, bromine 0020 allows a better coverage of the seeds: and/or chlorine dioxide; 0021 helps drying the seeds after having applied the 0032 wherein (ii) said at least one agriculturally accept aqueous solution; able solvent is soluble in water (W); 0022 prevents the release of mucilage from seeds such 0033 wherein said at least one agriculturally acceptable as flax and chia, and thus maintains the nutritional Solvent is preferably an agriculturally acceptable alcohol, value and natural composition of the seeds intact; glycol ether, propylene glycol, or ethylene glycol, and much 0023 maintains organoleptic properties of said seeds: more preferably an alcohol of formula ROH where R and/or represents a linear alkyl group having from 1 to 6 carbon 0024 shows an unexpected better efficiency. atoms, or a branched alkyl group having from 3 to 6 carbon 0025. Also, concerning the prevention of mucilage from atoms; wherein the water-soluble ingredients (CWSI) is seeds, it is to be noted that a person skilled in the art, would dissolved in the water (W), in a weight ratio (CWSI)/(W) not have been motivated to use a composition containing ranging from 1:100 to 1:4, and water on seeds Such as flax, which are recognized to produce mucilage when contacted with water. Surprisingly, the aque 0034 wherein when present, said at least one agricultur ous, Synergistic composition according to the invention ally acceptable solvent represents from 2% by volume to prevents mucilage from seeds. Preferably, an extra amount 70% by volume, preferably about 50% by volume, of the of an agriculturally acceptable alcohol may be added to said total Volume of the Synergistic composition to be formed. aqueous, Synergistic composition in order to further reduce 0035. According to another embodiment, the invention the amount of water in Such case. Such an extra amount of relates to a second composition of water-soluble ingredients an agriculturally acceptable alcohol does not affect the (CWSI) which when solubilized in water (W) and in the synergy mentioned hereinabove. presence of a wetting agent, forms a synergistic composition 0026. Also, it is worth mentioning that the Applicant has useful for the control of pathogens and/or the prevention of Surprisingly discovered that contact of the aqueous, Syner diseases associated with the presence of said pathogens in gistic composition with the seeds can be extended up to 12 and/or on seeds, preferably seeds having external shells, to 72 hours, thereby providing a better efficiency in reducing more preferably edible seeds and much more preferably the pathogens. Optionally, in the case of extended contact of hemp, flax and chia seeds, the seeds with the aqueous synergistic composition, it may 0036 wherein (i) said composition of water-soluble be advantageous to lower the humitidy content of the seeds ingredients (CWSI) comprises said wetting agent and at below 10% after the treatment with the synergistic solution least one oxidizer in liquid form or Solid form, or a precursor (in order to prevent regrowth of pathogens in and/or on the thereof in liquid or solid form, and preferably seeds). The regulation of the humidity content of seeds can 0037 liquidqu1d ppreformed pperacetic acid and/or in-situ be achieved by any appropriate means well known to person generated peracetic acid from powder precursors; and/ skilled in the art. O US 2016/0295860 A1 Oct. 13, 2016

0038 liquid hydrogen peroxide and/or hydrogen per 0050 liquid preformed peracetic acid and/or in-situ oxide released from a powder persalt, and/or generated peracetic acid from powder precursors; and/ 0039 other liquid oxidizers and/or powder oxidizers O Such as those generating iodine, chlorine, bromine 0051 liquid hydrogen peroxide and/or hydrogen per and/or chlorine dioxide; oxide released from a powder persalt, and/or wherein the water-soluble ingredients (CWSI) is dissolved 0.052 other liquid oxidizers and/or powder oxidizers in water (W), in a weight ratio (CWSI)/(W) ranging from Such as those generating iodine, chlorine, bromine and/or chlorine dioxide; 1:100 to 1:4. wherein (ii) said at least one agriculturally acceptable sol 0040. According to another embodiment, the invention vent is soluble in water (W); relates to the second composition defined hereinabove, 0053 wherein said at least one agriculturally acceptable wherein the at least one wetting agent is Solvent is preferably an agriculturally acceptable alcohol, 0041 an anionic surfactant selected from the group glycol ether, propylene glycol, or ethylene glycol, and much consisting of carboxylates, Sulfonates, petroleum Sul more preferably an alcohol of formula ROH where R fonates, alkylbenzenesulfonates, naphthalene Sul represents a linear alkyl group having from 1 to 6 carbon fonates, olefin Sulfonates, alkyl sulfates, sulfated natu atoms, or a branched alkyl group having from 3 to 6 carbon ral oils, sulfated natural fats, sulfated esters, sulfated atoms; alkanolamides, alkylphenol ethoxylates and Sulfated 0054 wherein the water-soluble ingredients (CWSI) is alkylphenols; dissolved in the water (W), in a weight ratio (CWSI)/(W) 0042 a non-ionic surfactant selected from the group ranging from 1:100 to 1:4, and consisting of alcohol ethoxylates, ethoxylated aliphatic 0055 wherein said at least one agriculturally acceptable alcohols, polyoxyethylene Surfactants, carboxylic solvent represents from 2% by volume to 70% by volume, esters, polyethylene glycol esters, anhydrosorbitol ester preferably about 50% by volume, of the total volume of the and its ethoxylated derivatives, glycol esters of fatty synergistic composition to be formed. acids, carboxylic amides, monoalkanolamine conden 0056. According to another embodiment, the invention sates and polyoxyethylene fatty acid amides; relates to the third composition defined hereinabove, which 0043 a cationic surfactant selected from the group when solubilized in water (W) and at least one agriculturally consisting of quaternary ammonium salts, amines with acceptable solvent, forms a synergistic composition for the amide linkages, polyoxyethylene alkyl and alicyclic control of pathogens and/or the prevention of diseases amines, 4-N.N.N',N'-tetrakis substituted ethylenedi associated with the presence of said pathogens in and/or on amines and 5.2-alkyl-1-hydroxyethyl 2-imidazolines; seeds, wherein said composition of water-soluble ingredi 0044) an amphoteric surfactant selected from the group ents (CWSI) is to be dissolved in water in a weight ratio consisting of N-coco 3-aminopropionic acid and its (CWSI)/(W) in the range from 1:100 to 1:4: Sodium salt, N-tallow 3-iminodipropionate and its diso 0057 wherein (i) said composition of water-soluble dium salt, N-carboxymethyl N-dimethyl N-9 octadece ingredients (CWSI) is nyl ammonium hydroxide, and N-cocoamidethyl N-hy 0.058 a liquid preformed peracetic acid, or droxyethylglycine and its sodium salt; or 0059 an in situ generated peracetic acid obtained from a 0045 an organic biodegradable surfactant obtained powdered composition of water-soluble ingredients from a natural Source Such as polyglycosides. (PCWSI) defining a peracetic acid precursor and consisting 0046 According to another embodiment, the invention of a dry, mixture of: relates to the second composition defined hereinabove, 0060 (i)-a) 30-60% w/w of a solid hydrogen peroxide wherein the at least one wetting agent is an alkyl polygly precursor, coside surfactant, preferably manufactured from fatty alco 0061 (i)-b) 10-40% w/w of a pH adjusting agent hols and glucose/dextrose, and more preferably Alkyl which is an organic acid or an inorganic acid, and polyglycoside APG 325NR liquid or GLUCOPONR) 50 G 0062 (i)-c) 10-40% w/w of an acetylating agent; and powder. wherein said peracetic acid precursor generates in situ peracetic acid (PAA) when the powdered composition 0047 According to another embodiment, the invention of water-soluble ingredients (PCWSI) is contacted with relates to the second composition defined hereinabove, water (W) in a weight ratio (PCWSI)/(W) ranging from wherein the wetting agent represents up to 25% by weight of 1:100 to 1:4, being understood that when 2 g of said the weight of the oxidizer, preferably about 4% by weight dry, water-soluble mixture of (i)-a), (i)-b), (i)-c) is 0048. According to another embodiment, the invention admixed with 1000 g of water, 100 to 250 ppm of relates to a third composition of water-soluble ingredients peracetic acid (PAA) are generated in situ at pH 9.0+3; (CWSI) which when solubilized in water (W) and at least and one agriculturally acceptable solvent, forms a synergistic 0063 wherein (ii) said at least one agriculturally accept composition useful for the control of pathogens and/or the able solvent is soluble in water (W) selected from the group prevention of diseases associated with the presence of said consisting of an agriculturally acceptable alcohol, glycol pathogens in and/or on seeds, preferably seeds having exter ether, propylene glycol and ethylene glycol, and represents nal shells, more preferably edible seeds and much more from 2% by volume to 70% by volume of the total volume preferably hemp, flax and chia seeds, of the Synergistic composition to be formed. 0049 wherein (i) said composition of water-soluble 0064. According to another embodiment, the invention ingredients (CWSI) comprises at least one oxidizer in liquid relates to the third composition defined hereinabove, form or solid form, or a precursor thereof in liquid or solid wherein the at least one oxidizer is the liquid preformed form, and preferably peracetic acid and/or the in-situ generated peracetic acid US 2016/0295860 A1 Oct. 13, 2016

from the powder precursors, preferably the in-situ generated 0.077 a. According to another embodiment, the inven peracetic acid from the powder precursors. tion relates to the third composition defined herein 0065 According to another embodiment, the invention above, wherein the composition of water-soluble ingre relates to the third composition defined hereinabove, dients (CWSI) is a powdered composition of water wherein the at least one agriculturally acceptable alcohol is soluble ingredients (PCWSI) comprising: further a food-grade alcohol which is listed in FDA's CFR 0078 (i) a peracetic acid precursor comprising: 21 as Generally Regarded as Safe (GRAS) for use in food 0079 a) a solid hydrogen peroxide precursor, (section 184.1293), preferably ethanol, propanol or isopro 0080 b) optionally a pH adjusting agent, and panol, and more preferably a food graded ethanol. 0081 c) an acetylating agent; and 0066. According to another embodiment, the invention wherein said peracetic acid precursor generates in situ relates to the third composition defined hereinabove, peracetic acid (PAA) when contacted water (W) in a weight wherein said composition of water-soluble ingredients fur ratio (PCWSI)/(W) ranging from 1:100 to 1:4, forms a ther comprises: corresponding solution of peracetic acid, the at least one 0067 (iii) at least one wetting agent. agriculturally acceptable solvent forming a mixture with the 0068 According to another embodiment, the invention water (W) or being preferably added to the solution of relates to the third composition defined hereinabove, peracetic acid, to form the synergistic composition. wherein the at least one wetting agent is I0082. According to another embodiment, the invention 0069 an anionic surfactant selected from the group relates to the third composition defined hereinabove, consisting of carboxylates, Sulfonates, petroleum Sul wherein the at least one agriculturally acceptable alcohol is fonates, alkylbenzenesulfonates, naphthalene Sul a food-grade alcohol which is listed in FDA's CFR 21 as fonates, olefin Sulfonates, alkyl sulfates, sulfated natu Generally Regarded as Safe (GRAS) for use in food (section ral oils, sulfated natural fats, sulfated esters, sulfated 184.1293), preferably ethanol, propanol or isopropanol, and alkanolamides, alkylphenol ethoxylates and Sulfated more preferably a food graded ethanol. alkylphenols; I0083. According to another embodiment, the invention 0070 a non-ionic surfactant selected from the group relates to the third composition defined hereinabove, consisting of alcohol ethoxylates, ethoxylated aliphatic wherein said powdered composition composition of water alcohols, polyoxyethylene Surfactants, carboxylic soluble ingredients (PCWSI) further comprises at least one esters, polyethylene glycol esters, anhydrosorbitol ester Wetting agent. and its ethoxylated derivatives, glycol esters of fatty I0084. According to another embodiment, the invention acids, carboxylic amides, monoalkanolamine conden relates to the third composition defined hereinabove, sates and polyoxyethylene fatty acid amides; wherein the wetting agent is 0071 a cationic surfactant selected from the group 0085 an anionic surfactant selected from the group consisting of quaternary ammonium salts, amines with consisting of carboxylates, Sulfonates, petroleum Sul amide linkages, polyoxyethylene alkyl and alicyclic fonates, alkylbenzenesulfonates, naphthalene Sul fonates, olefin Sulfonates, alkyl sulfates, Sulfated natu amines, 4-N.N.N',N'-tetrakis substituted ethylenedi ral oils, sulfated natural fats, sulfated esters, sulfated amines and 5.2-alkyl-1-hydroxyethyl 2-imidazolines; alkanolamides, alkylphenol ethoxylates and Sulfated 0072 an amphoteric surfactant selected from the group alkylphenols; consisting of N-coco 3-aminopropionic acid and its I0086 a non-ionic surfactant selected from the group Sodium salt, N-tallow 3-iminodipropionate and its diso consisting of alcohol-ethoxylates, ethoxylated aliphatic dium salt, N-carboxymethyl N-dimethyl N-9 octadece alcohols, polyoxyethylene Surfactants, carboxylic nyl ammonium hydroxide, and N-cocoamidethyl N-hy esters, polyethylene glycol esters, anhydrosorbitol ester droxyethylglycine and its sodium salt; or and its ethoxylated derivatives, glycol esters of fatty 0073 an organic biodegradable surfactant obtained acids, carboxylic amides, monoalkanolamine conden from a natural Source Such as polyglycosides. sates and polyoxyethylene fatty acid amides; 0074 According to another embodiment, the invention 0.087 a cationic surfactant selected from the group relates to the third composition defined hereinabove, consisting of quaternary ammonium salts, amines with wherein the at least one wetting agent is an alkyl polygly amide linkages, polyoxyethylene alkyl and alicyclic coside surfactant, preferably manufactured from fatty alco amines, 4-N.N.N',N'-tetrakis substituted ethylenedi hols and glucose/dextrose, and more preferably Alkyl amines and 5.2-alkyl-1-hydroxyethyl 2-imidazolines; polyglycoside APG 325NR liquid or GLUCOPONR) 50 G 0088 an amphoteric surfactant selected from the group powder. consisting of N-coco 3-aminopropionic acid and its 0075 According to another embodiment, the invention Sodium salt, N-tallow 3-iminodipropionate and its diso relates to the third composition defined hereinabove, dium salt, N-carboxymethyl N-dimethyl N-9 octadece wherein the wetting agent represents up to 25% by weight of nyl ammonium hydroxide, and N-cocoamidethyl N-hy the weight of the oxidizer, preferably about 4% by weight droxyethylglycine and its sodium salt; or 0076 According to another embodiment, the invention 0089 an organic biodegradable surfactant obtained relates to the third composition defined hereinabove, from natural source Such as polyglycosides. wherein the at least one additional agriculturally acceptable 0090 According to another embodiment, the invention solvent is selected from the group consisting of C-C, relates to the third composition defined hereinabove, alcohols and glycol ethers, more particularly from the group wherein the at least one wetting agent is alkyl polyglycoside consisting of ethanol, isopropanol and monobutyl ether of surfactant, preferably manufactured from fatty alcohols and ethylene glycol, and much more preferably being food grade glucose/dextrose, and more preferably Alkyl polyglycoside ethanol. APG 325NR liquid or GLUCOPONR 50 G powder. US 2016/0295860 A1 Oct. 13, 2016

0091. According to another embodiment, the invention dipicolinic acid, urea.HCl, boric acid, or mixtures thereof, relates to the third composition defined hereinabove, and more preferably citric acid. wherein the wetting agent represents up to 25% by weight of 0105. According to another embodiment, the invention the weight of the peracetic precursor, preferably about 4% relates to a first synergistic composition for the control of by weight pathogens and/or the prevention of diseases associated with 0092. According to another embodiment, the invention the presence of said pathogens in and/or on seeds, preferably relates to the third composition defined hereinabove, seeds having external shells, more preferably edible seeds wherein the peracetic acid precursor is a dry, water-soluble and much more preferably hemp, flax and chia seeds, mixture of: wherein said synergistic composition comprises: (0093 (i)-a) 30-60% w/w of the solid hydrogen perox 0106 water (W), ide precursor, 0.107 the first composition of water-soluble ingredi 0094 (i)-b) 10-40% w/w the pH adjusting agent, and ents (CWSI) defined hereinabove, and 0.108 at least one wetting agent soluble in water (W), 0.095 (i)-c) 10-40% w/w of the acetylating agent; or preferably at least one agriculturally acceptable wherein (i)-a), (i)-b) and (i)-c) represent the peracetic acid solvent which is soluble in water (W), or more prefer precursor, and wherein when 2 g of said dry, water-soluble ably at least one wetting agent soluble in water (W) and mixture of (i)-a), (i)-b), (i)-c) is admixed with 1000 g of preferably at least one agriculturally acceptable solvent water, 100 to 250 ppm of peracetic acid (PAA) are generated which is soluble in water (W); in situ at pH 9.0+3. said at least one agriculturally acceptable solvent being 0096. According to another embodiment, the invention preferably an agriculturally acceptable alcohol, glycol ether, relates to the third composition defined hereinabove, propylene glycol, or ethylene glycol, and much more pref wherein said powdered composition of water-soluble ingre erably an alcohol of formula ROH where R represents a dients (PCWSI) comprises is a dry, water-soluble mixture linear alkyl group having from 1 to 6 carbon atoms, or a of: branched alkyl group having from 3 to 6 carbon atoms; 0097. 1. about 58% w/w of the solid hydrogen peroxide 0109 wherein the composition of water-soluble ingredi precursor, ents (CWSI) is contacted with water (W), in a weight ratio 0098 2. about 18% w/w the pH adjusting agent, (CWSI)/(W) ranging from 1:100 to 1:4, preferably 1:5, 0099 3. about 20% w/w of the acetylating agent; wherein, when present, the at least one agriculturally accept 0100. 4. about 4% w/w of a wetting agent as defined able solvent either forming a mixture with the water (W) or hereinabove; and being preferably added to a solution of peracetic acid wherein (1), (2) and (3) represent the peracetic acid precur resulting from the contact of the composition of water sor; and wherein when 2 g of said dry, water-soluble mixture soluble ingredients (CWSI) with water, to form the syner of (1), (2), (3) and (4) is admixed with 1000 g of water, 100 gistic composition; and to 250 ppm of peracetic acid (PAA) are generated in situ at 0110 wherein when present, the at least one agricultur pH 9.0+3. ally acceptable solvent is present in the synergistic compo 0101. According to another embodiment, the invention sition in Such an amount that it represents from 2% by relates to the third composition defined hereinabove, volume to 70% by volume, preferably about 50% by vol wherein the peracetic acid precursor generates about 200 ume, of the total Volume of the Synergistic composition. ppm of peracetic acid (PAA). 0111. According to another embodiment, the invention 0102) According to another embodiment, the invention relates to a second synergistic composition for the control of relates to any one of the compositions defined hereinabove, pathogens and/or the prevention of diseases associated with wherein the acylating agent is acetylsalicylic acid or tet the presence of said pathogens in and/or on seeds, preferably raacetylethylenediamine (TAED), preferably tetraacetyleth seeds having external shells, more preferably edible seeds ylenediamine (TAED). and much more preferably hemp, flax and chia seeds, 0103) According to another embodiment, the invention wherein said synergistic composition comprises: relates to any one of the compositions defined hereinabove, 0112 water (W), wherein the Solid hydrogen peroxide precursor is a persalt, 0113 the second composition of water-soluble ingre preferably sodium perborate, Sodium percarbonate, ammo dients (CWSI) defined hereinabove, and nium percarbonate, sodium peroxyhydrate, calcium peroX 0114 at least one wetting agent soluble in water (W); ide, sodium peroxide, Sodium perborate monohydrate, wherein the composition of water-soluble ingredients Sodium perborate tetrahydrate, Sodium persulfate, potassium (CWSI) is contacted with water (W), in a weight ratio monopersulfate, perphosphate, magnesium peroxide, Zinc (CWSI)/(W) ranging from 1:100 to 1:4, preferably 1:5. peroxide, urea hydrogen peroxide, perhydrate of urea, thio 0115 According to another embodiment, the invention urea dioxide, or mixtures thereof, and more preferably relates to a third synergistic composition for the control of Sodium percarbonate or ammonium percarbonate, and much pathogens and/or the prevention of diseases associated with more preferably sodium percarbonate. the presence of said pathogens in and/or on seeds, preferably 0104. According to another embodiment, the invention seeds having external shells, more preferably edible seeds relates to any one of the compositions defined hereinabove, and much more preferably hemp, flax and chia seeds, wherein the pH adjusting agent is an organic acid or an wherein said synergistic composition comprises: inorganic acid, preferably Sulfuric acid, citric acid, phos 0.116) water (W), phoric acid, nitric acid, hydrochloric acid, glycolic acid, 0.117 the third composition of water-soluble ingredi formic acid, acetic acid, hydrofluoric acid, nitrous acid, ents (CWSI) defined hereinabove, and hydrocyanic acid, benzoic acid, carboxylic acid, lactic acid, 0118 at least one agriculturally acceptable solvent acetic acid, oxalic acid, Sulfa mic acid, phosphorous acid, which is soluble in water (W), said at least one agri US 2016/0295860 A1 Oct. 13, 2016

culturally acceptable solvent being preferably an agri above, wherein when the at least one additional agricultur culturally acceptable alcohol, glycol ether, propylene ally acceptable solvent is selected from the group consisting glycol, or ethylene glycol, and much more preferably of C-C alcohols and glycol ethers, more particularly from an alcohol of formula ROH where R represents a linear the group consisting of ethanol, isopropanol and monobutyl alkyl group having from 1 to 6 carbon atoms, or a ether of ethylene glycol, and much more preferably being branched alkyl group having from 3 to 6 carbon atoms; food grade ethanol, and said aqueous synergistic composi 0119 wherein the composition of water-soluble ingredi tion further prevents the release of mucilage from seeds, ents (CWSI) is contacted with water (W), in a weight ratio especially on flax seeds and chia seeds. (CWSI)/(W) ranging from 1:100 to 1:4, preferably 1:5, the I0126. According to another embodiment, the invention at least one agriculturally acceptable solvent either forming relates to any one of the Synergistic compositions defined a mixture with the water (W) or being preferably added to hereinabove, wherein said synergistic composition is for an a solution of peracetic acid resulting from the contact of the application to the seeds by spraying, vaporizing, soaking, composition of water-soluble ingredients (CWSI) with fumigating, or electrostatic spraying, preferably spraying. water, to form the synergistic composition; and I0127. According to another embodiment, the invention 0120 wherein the at least one agriculturally acceptable relates to any one of the Synergistic compositions defined Solvent being present in the synergistic composition in Such hereinabove, wherein the pathogens selected from the group an amount that it represents from 2% by volume to 70% by consisting of viruses, bacteria, fungi, yeasts and moulds. volume, preferably about 50% by volume, of the total Volume of the synergistic composition. I0128. According to another embodiment, the invention 0121 According to another embodiment, the invention relates to the third synergistic composition defined herein relates to the third synergistic composition defined herein above, for the further prevention of mucilage from seeds. above, wherein the composition of water-soluble ingredients I0129. According to another embodiment, the invention (CWSI) is contacted with water (W), in a weight ratio relates to any one of the Synergistic compositions defined (CWSI)/(W) ranging from 1:100 to 1:4, preferably 1:5, the hereinabove, wherein pathogens are bacteria, preferably at least one agriculturally acceptable solvent being added to bacteria are selected from the group consisting of E. Coli, a solution of peracetic acid resulting from the contact of the Listeria monocytogenes and Salmonella spp., and more composition of water-soluble ingredients (CWSI) with preferably E. coli in hemp seed and Salmonella in chia water, to form the synergistic composition. seeds. 0122) According to another embodiment, the invention 0.130. According to another embodiment, the invention relates to any one of the Synergistic compositions defined relates to any one of the Synergistic compositions defined hereinabove, wherein the at least one oxidizer is the pre hereinabove, wherein pathogens cause disease in crop formed liquid peracetic acid, wherein the volume ratio of the plants; said pathogens are bacteria (including but not limited preformed liquid peracetic to the water (W) is about 1:5, and to Agrobacterium spp., Burkholderia spp., Clavibacter spp., wherein said synergistic composition has about 5% by Corynebacterium spp., Erwinia spp., Pseudomonas spp., weight of dissolved peracetic acid with respect to the total Ralstonia spp., Rhizomonas spp., Xanthomonas spp., and weight of the synergistic composition. Xylella spp.). 0123. According to another embodiment, the invention I0131. According to another embodiment, the invention relates to any one of the Synergistic compositions defined relates to any one of the Synergistic compositions defined hereinabove, wherein the at least one oxidizer is the pre hereinabove, wherein pathogens cause disease in crop formed liquid peracetic which have the following formula plants; said pathogens are fungi (including but not limited to tion: Albugo spp., Alternaria spp., Armillaria spp., Aspergillus spp., Athelia spp., Bipolaris spp., Botryosphaeria spp., Botryotinia spp., Botrytis spp., Bremia spp., Capnodium Ingredients Percentage (ww) spp., Ceratobasidium spp., Ceratocystis spp., Cercospora Distilled Water 21.0% spp., Choanephora spp., Claviceps spp., CorynespOra spp., Acetic Acid 92% (CAS number: 64-19-7) 13.5% Cronartium spp., Cryphonectria spp., Cylindrocladium spp., Sulphuric Acid 96% (CAS number: 7664-93-9) 1.1% Hydrogen Peroxide 50% (CAS number: 7722-84-1) 62.0% Cytospora spp., Diaporthe spp., Dipodia spp., Dreschlera Citric acid - Food grade (CAS number: 77-92-9) O.9% spp., Elsinoe spp., ErexOhilum spp., Erysiphe spp., Eutylpha APG 325N (R) (Polyglycoside surfactant) 1.5% spp., Exobasidium spp., Fusarium spp., Gaeumannomyces CAS number: 110615-47-9: spp., Gliocladium spp., Gymnosporangium spp., Heteroba sidium spp., Hypoxylon spp., Kutilakesa spp., Lophioder TOTAL: 100% ww. mium spp., Magnaporthe spp., Melampsora spp., Monilinia spp., Mycosphaerella spp., Myrothecia spp., Nectriella spp., 0.124. According to another embodiment, the invention Nematospora spp., Oidium spp., Olipidium spp., Ophiostoma relates to any one of the Synergistic compositions defined spp., Penicillium spp., Peronospora spp., Phakospora spp., hereinabove, wherein the composition of water-soluble Phoma spp., Phomopsis spp., Phragmidium spp., Phyllac ingredients (CWSI) is a powdered composition of water tinia spp., Physoderma spp., Phytophthora spp., Plasmodio soluble ingredients (PCWSI) as defined hereinabove, and phora spp., Plasmopara spp., Pseudoperonospora spp., wherein the powdered composition of water-soluble ingre Puccinia spp., Pythium spp., Rhizoctonia spp., Rhizopus dients (PCWSI) is dissolved in water (W) in the weight ratio spp., Rhytisma spp., Sclerotinia spp., Sclerotium spp., Spon (PCWSI)/(W) ranging from 1:100 to 1:4, preferably about gospora spp., Synchytrium spp., Taphrina spp., Thanatepho 1:10. rus spp., Thielaviopsis spp., Tilletia spp., Uncinula spp., 0.125. According to another embodiment, the invention Urocystis spp., Ustilago spp., Valsa spp., Venturia spp., relates to the third synergistic composition defined herein Verticillium spp., and Xwlaria spp.). US 2016/0295860 A1 Oct. 13, 2016

0.132. According to another embodiment, the invention keluak or kluwak or kepayang, kokam seed, korarima relates to any one of the Synergistic compositions defined or Ethiopian cardamom or false cardamom, mace, hereinabove, wherein the seeds are edible seeds, grains raw mahlab or Saint Lucie cherry, black mustard seed, and/or cooked, seeds of transplantable crops, or seeds for brown mustard seed, white mustard seed, yellow mus Sprouting. Such as: tard seed, nigella or kalonji or black caraway or black 0.133 cereals such as: true cereals which are seeds of onion seed, njangsa or djansang, nutmeg, black pepper certain species of grass: maize, wheat and rice, said seed, green pepper seed, black pepper seed, white cereal including and being not limited to barley, fonio, pepper seed, star anise, Sumac, Szechuan pepper or maize (corn), pearl millet, oats, palmer's grass, rice, Sichuan pepper, Vanilla, wattleseed; rye, Sorghum, spelt, teff, triticale, wheat or wild rice; 0.141 seeds of crops transplantable from greenhouse to I0134) pseudocereals such as breadnut, buckwheat, cat field, including but not limited to basil, bell pepper, tail, chia, flax, grain amaranth, kafiiwa, pitseed goose broccoflower, broccoli, brussels sprouts, cabbage, can foot, quinoa or wattleseed (also called acacia seed); taloupe, cauliflower, celery, cucumber, eggplant, head 0.135 nuts such as for example culinary nuts (including lettuce, honeydew, muskmelon, onion, radicchio, and not limited to almonds, coconuts, peanuts and romaine lettuce, squash, tobacco, tomato, watermelon; cashews) and nuts such as almond, beech, brazil nut, O candlenut, cashew, chestnuts (including and not limited 0.142 medical marijuana. to Chinese chestnut or Sweet chestnut), coconut, colo 0.143 According to another embodiment, the invention cynth, Cucurbita ficifolia, filbert, Gevulina avellana, relates to any one of the Synergistic compositions defined hickory (including and not limited to pecan or shagbark hereinabove, wherein the seeds are edible post-harvest hickory), Terminalia catappa, hazelnut, Indian beech, seeds, spices and grains, and preferably whole dried pear, kola nut, macadamia, Malabar chestnut, pistacia, mam oncillo, maya nut, mongongo, oak acorns, ogbono nut, split pea dried, pea fiber, oregano, Vanilla, basil, black paradise nut, pili nut, walnut (including and not limited pepper, chia, sesame, sprouted flax and chia, flax and hemp, to black walnut) or water caltrop: and more preferably hemp, flax and chia seeds. 0.136 nut-like gymnosperm seeds such as pine nuts are 0144. According to another embodiment, the invention gymnosperm seeds that are edible (including and not relates to a first method for the control of pathogens and/or limited to cycads, ginkgo, Gnetum gnemon, juniper, the prevention of diseases associated with the presence of monkey-puzzle, pine nuts, or podocarps): said pathogens in and/or on seeds, preferably seeds having 0.137 other seeds such as cempedak, coffee, egusi, external shells, more preferably edible seeds such as hemp, euryale feroX (fox nut), fluted pumpkin, hemp seed, flax and chia seeds, said method comprising a step of jackfruit, lotus seed, Malabar gourd, pumpkin seed, contacting said seeds with the first synergistic composition Sunflower seed, sesame seed or Tahini; defined hereinabove, wherein the Synergistic composition is 0.138 beans Such as bambara groundnut, chickpeas, contacted with seeds. cowpeas (including and not limited to black eyed pea), 0145 According to another embodiment, the invention dry beans, fava or broad beans, hyacinth bean, lablab. relates to a second method for the control of pathogens lentils, lupins, Moringa oleifera, peas, peanuts, pigeon and/or the prevention of diseases associated with the pres peas, Sterculia, Velvet beans, winged beans, yam beans ence of said pathogens in and/or on seeds, preferably seeds or soybeans; having external shells, more preferably edible seeds such as 0.139 seeds for sprouting, including, but not limited to, hemp, flax and chia seeds, said method comprising a step of pulses or legumes (such as, but not limited to, alfalfa, contacting said seeds with the second synergistic composi clover, fenugreek, lentil, pea, chickpea, mung bean and tion defined hereinabove, wherein the synergistic composi Soybean; cereals (such as, but not limited to, oat, wheat, tion is contacted with seeds in Such an amount that said at maize (corn), rice, barley, rye, kamut, quinoa, amaranth least one agriculturally acceptable solvent represents less and buckwheat, oilseeds (such as, but not limited to, than 10% by volume, preferably from 1 to 5% by volume, Sesame, Sunflower, almond, hazelnut, linseed, peanut); and more preferably about 2% by volume as final concen brassicas or crucifers or cabbage family (such as, but tration of the at least one agriculturally acceptable solvent not limited to, broccoli, cabbage, watercress, mustard, admixed with seeds. miZuna, radish, daikon (kaiware), rocket (arugula), 0146 According to another embodiment, the invention tatsoi and turnip); umbelliferous vegetables or parsley relates to a third method for the control of pathogens and/or family (such as, but not limited to, carrot, celery, the prevention of diseases associated with the presence of fennel, parsley; alliums or onion family (such as, but said pathogens in and/or on seeds, preferably seeds having not limited to, onion, leek, green onion or me-negi); external shells, more preferably edible seeds such as hemp, other vegetables and herbs (such as, but not limited to, flax and chia seeds, said method comprising a step of spinach, lettuce, milk thistle and lemon grass); contacting said seeds with the third synergistic composition 0140 seed spices including, but not limited to awain defined hereinabove, wherein the Synergistic composition is or carom seeds, alligator pepper or mbongo spice or contacted with seeds in Such an amount that said at least one mbongochobi or hepper pepper, allspice, anise, aniseed agriculturally acceptable solvent represents less than 10% by myrtle, annatto, borage, black cardamom, black mus volume, preferably from 1 to 5% by volume, and more tard, blue fenugreek or blue melilot, brown mustard, preferably about 2% by volume as final concentration of the caraway, cardamom, celery seed, clove, coriander seed, at least one agriculturally acceptable solvent admixed with cumin, dill seed, fennel, fenugreek, grains of paradise, seeds. grains of Selim or Kani pepper, juniper berry, kala 0147 According to another embodiment, the invention Zeera or kala jira or black cumin, kawakawa seeds, relates to the any one of the methods defined hereinabove, US 2016/0295860 A1 Oct. 13, 2016

wherein the pathogens selected from the group consisting of candlenut, cashew, chestnuts (including and not limited viruses, bacteria, fungi, yeasts and moulds. to Chinese chestnut or Sweet chestnut), coconut, colo 0148. According to another embodiment, the invention cynth, Cucurbita ficifolia, filbert, Gevulina avellana, relates to the third method defined hereinabove, wherein the hickory (including and not limited to pecan or shagbark pathogens selected from the group consisting of viruses, hickory), Terminalia catappa, hazelnut, Indian beech, bacteria, fungi, yeasts and moulds, and further for the kola nut, macadamia, Malabar chestnut, pistacia, mam prevention of mucilage from seeds. oncillo, maya nut, mongongo, oak acorns, ogbono nut, 0149 According to another embodiment, the invention paradise nut, pili nut, walnut (including and not limited relates to any one of the methods defined hereinabove, to black walnut) or water caltrop: wherein pathogens are bacteria, preferably bacteria are 0156 nut-like gymnosperm seeds such as pine nuts are selected from the group consisting of E. Coli, Listeria gymnosperm seeds that are edible (including and not monocytogenes and Salmonella spp., and more preferably E. limited to cycads, ginkgo, Gnetum gnemon, juniper, coli on hemp seeds and Salmonella on chia seeds. monkey-puzzle, pine nuts, or podocarps): 0150. According to another embodiment, the invention relates to any one of the methods defined hereinabove, 0157 other seeds such as cempedak, coffee, egusi, wherein pathogens cause disease in crop plants; said patho euryale feroX (fox nut), fluted pumpkin, hemp seed, gens are bacteria (including but not limited to Agrobacte jackfruit, lotus seed, Malabar gourd, pumpkin seed, rium spp., Burkholderia spp., Clavibacter spp., Corynebac Sunflower seed or sesame seed; terium spp., Erwinia spp., Pseudomonas spp., Ralstonia 0158 beans such as bambara groundnut, chickpeas, spp., Rhizomonas spp., Xanthomonas spp., and Xvlella spp.). cowpeas (including and not limited to black eyed pea), 0151. According to another embodiment, the invention dry beans, fava or broad beans, hyacinth bean, lablab. relates to any one of the methods defined hereinabove, lentils, lupins, Moringa oleifera, peas, peanuts, pigeon wherein pathogens cause disease in crop plants; said patho peas, Sterculia, Velvet beans, winged beans, yam beans gens are fungi (including but not limited to Albugo spp., or soybeans; Alternaria spp., Armillaria spp., Aspergillus spp., Athelia spp., Bipolaris spp., Botryosphaeria spp., Botryotinia spp., 0159 seeds for sprouting, including, but not limited to, Botrytis spp., Bremia spp., Capnodium spp., Ceratoba pulses or legumes (such as, but not limited to, alfalfa, sidium spp., Ceratocystis spp., Cercospora spp., Choane clover, fenugreek, lentil, pea, chickpea, mung bean and phora spp., Claviceps spp., Corynespora spp., Cronartium Soybean; cereals (such as, but not limited to, oat, wheat, spp., Cryphonectria spp., Cylindrocladium spp., Cytospora maize (corn), rice, barley, rye, kamut, quinoa, amaranth spp., Diaporthe spp., Dipodia spp., Dreschlera spp., Elsinoe and buckwheat, oilseeds (such as, but not limited to, spp., Erexohilum spp., Erysiphe spp., Eutylpha spp., Exoba Sesame, Sunflower, almond, hazelnut, linseed, peanut); sidium spp., Fusarium spp., Gaeumannomyces spp., Glio brassicas or crucifers or cabbage family (Such as, but cladium spp., Gymnosporangium spp., Heterobasidium spp., not limited to, broccoli, cabbage, watercress, mustard, Hypoxy/on spp., Kutilakesa spp., Lophiodermium spp., miZuna, radish, daikon (kaiware), rocket (arugula), Magnaporthe spp., Melampsora spp., Monilinia spp., tatsoi and turnip); umbelliferous vegetables or parsley Mycosphaerella spp., Myrothecia spp., Nectriella spp., family (such as, but not limited to, carrot, celery, Nematospora spp., Oidium spp., Olipidium spp., Ophiostoma fennel, parsley; alliums or onion family (such as, but spp., Penicillium spp., Peronospora spp., Phakospora spp., not limited to, onion, leek, green onion or me-negi); Phoma spp., Phomopsis spp., Phragmidium spp., Phyllac other vegetables and herbs (such as, but not limited to, tinia spp., Physoderma spp., Phytophthora spp., Plasmodio spinach, lettuce, milk thistle and lemon grass); phora spp., Plasmopara spp., Pseudoperonospora spp., 0.160 seed spices including, but not limited to ajwain Puccinia spp., Pythium spp., Rhizoctonia spp., Rhizopus or carom seeds, alligator pepper or mbongo spice or spp., Rhytisma spp., Sclerotinia spp., Sclerotium spp., Spon mbongochobi or hepper pepper, allspice, anise, aniseed gospora spp., Synchytrium spp., Taphrina spp., Thanatepho myrtle, annatto, borage, black cardamom, black mus rus spp., Thielaviopsis spp., Tilletia spp., Uncinula spp., tard, blue fenugreek or blue melilot, brown mustard, Urocystis spp., Ustilago spp., Valsa spp., Venturia spp., caraway, cardamom, celery seed, clove, coriander seed, Verticillium spp., and Xvlaria spp.). cumin, dill seed, fennel, fenugreek, grains of paradise, 0152. According to another embodiment, the invention grains of Selim or Kani pepper, juniper berry, kala relates to any one of the methods defined hereinabove, Zeera or kala jira or black cumin, kawakawa seeds, wherein the seeds are edible seeds, grains raw and/or keluak or kluwak or kepayang, kokam seed, korarima cooked, seeds of transplantable crops, or seeds for sprouting, or Ethiopian cardamom or false cardamom, mace, Such as: mahlab or Saint Lucie cherry, black mustard seed, 0153 cereals such as: true cereals which are seeds of brown mustard seed, white mustard seed, yellow mus certain species of grass: maize, wheat and rice, said tard seed, nigella or kalonji or black caraway or black cereal including and being not limited to barley, fonio, onion seed, njangsa or djansang, nutmeg, black pepper maize (corn), pearl millet, oats, palmer's grass, rice, seed, green pepper seed, black pepper seed, white rye, Sorghum, spelt, teff, triticale, wheat or wild rice; pepper seed, star anise, Sumac, Szechuan pepper or 0154 pseudocereals such as breadnut, buckwheat, cat Sichuan pepper, Vanilla, wattleseed; tail, chia, flax, grain amaranth, kafiiwa, pitseed goose 0.161 seeds of crops transplantable from greenhouse to foot, quinoa or wattleseed (also called acacia seed); field, including but not limited to basil, bell pepper, 0155 nuts such as for example culinary nuts (including broccoflower, broccoli, brussels sprouts, cabbage, can and not limited to almonds, coconuts, peanuts and taloupe, cauliflower, celery, cucumber, eggplant, head cashews) and nuts such as almond, beech, brazil nut, lettuce, honeydew, muskmelon, onion, radicchio, US 2016/0295860 A1 Oct. 13, 2016

romaine lettuce, squash, tobacco, tomato, watermelon; bacteria (including but not limited to Agrobacterium spp., O Burkholderia spp., Clavibacter spp., Corynebacterium spp., 0162 medical marijuana. Erwinia spp., Pseudomonas spp., Ralstonia spp., Rhizomo 0163 According to another embodiment, the invention nas spp., Xanthomonas spp., and Xylella spp.). relates to any one of the methods defined hereinabove, 0.174. According to another embodiment, the invention wherein the seeds are seeds of whole dried pear, split pea relates to any one of uses defined hereinabove, wherein dried, pea fiber, oregano, Vanilla, basil, chia, Sesame, pathogens cause disease in crop plants; said pathogens are sprouted flax and chia, flax and hemp, and more preferably fungi (including but not limited to Albugo spp., Alternaria hemp seeds, flax seeds and chia seeds. spp., Armillaria spp., Aspergillus spp., Athelia spp., Bipo 0164. According to another embodiment, the invention laris spp., Botryosphaeria spp., Botryotinia spp., Botrytis relates to any one of the methods defined hereinabove, spp., Bremia spp., Capnodium spp., Ceratobasidium spp., wherein said synergistic composition is for an application to Ceratocystis spp., Cercospora spp., Choanephora spp., the seeds by spraying, vaporizing, soaking, fumigating, or Claviceps spp., Corynespora spp., Cronartium spp., Cry electrostatic spraying, preferably spraying. phonectria spp., Cylindrocladium spp., Cytospora spp., Dia 0.165 According to another embodiment, the invention porthe spp., Dipodia spp., Dreschlera spp., Elsinoe spp., relates to any one of the methods defined hereinabove, ErexOhilum spp., Erysiphe spp., Eutylpha spp., Exobasidium wherein said synergistic composition is for an application to spp., Fusarium spp., Gaeumannomyces spp., Gliocladium the seeds by spraying and keeping the synergistic compo spp., Gymnosporangium spp., Heterobasidium spp., sition in contact with the seeds and grains for 2 minutes to Hypoxylon spp., Kutilakesa spp., Lophiodermium spp., 48 hours and preferably 24 hours before drying. Magnaporthe spp., Melampsora spp., Monilinia spp., 0166 According to another embodiment, the invention Mycosphaerella spp., Myrothecia spp., Nectriella spp., relates to any one of the methods defined hereinabove, Nematospora spp., Oidium spp., Olipidium spp., Ophiostoma wherein seeds are dried after contact with the synergistic spp., Penicillium spp., Peronospora spp., Phakospora spp., composition and contact time, to reduce the moisture con Phoma spp., Phomopsis spp., Phragmidium spp., Phyllac tent of the seeds below 10% and further contribute to prevent tinia spp., Physoderma spp., Phytophthora spp., Plasmodio a regrowth of microorganisms. phora spp., Plasmopara spp., Pseudoperonospora spp., 0167 According to another embodiment, the invention Puccinia spp., Pythium spp., Rhizoctonia spp., Rhizopus relates to a first use of the first synergistic composition spp., Rhytisma spp., Sclerotinia spp., Sclerotium spp., Spon defined hereinabove, for the control of pathogens and/or the gospora spp., Synchytrium spp., Taphrina spp., Thanatepho prevention of diseases associated with the presence of said rus spp., Thielaviopsis spp., Tilletia spp., Uncinula spp., pathogens in and/or on seeds, preferably seeds having exter Urocystis spp., Ustilago spp., Valsa spp., Venturia spp., nal shells, more preferably edible seeds Such as hemp seeds, Verticillium spp., and Xwlaria spp.). chia seeds and flax seeds. 0.175. According to another embodiment, the invention 0168 According to another embodiment, the invention relates to any one of uses defined hereinabove, wherein the relates to a second use of the second synergistic composition seeds are edible seeds, grains raw and/or cooked, seeds of defined hereinabove, for the control of pathogens and/or the transplantable crops, or seeds for sprouting, Such as: prevention of diseases associated with the presence of said 0176) cereals such as: true cereals which are seeds of pathogens in and/or on seeds, preferably seeds having exter certain species of grass: maize, wheat and rice, said nal shells, more preferably edible seeds Such as hemp seeds, cereal including and being not limited to barley, fonio, chia seeds and flax seeds. maize (corn), pearl millet, oats, palmer's grass, rice, 0169. According to another embodiment, the invention rye, Sorghum, spelt, teff, triticale, wheat or wild rice; relates to a third use of the third synergistic composition 0.177 pseudocereals such as breadnut, buckwheat, cat defined hereinabove, for the control of pathogens and/or the tail, chia, flax, grain amaranth, kafiiwa, pitseed goose prevention of diseases associated with the presence of said foot, quinoa or wattleseed (also called acacia seed); pathogens in and/or on seeds, preferably seeds having exter 0.178 nuts such as for example culinary nuts (including nal shells, more preferably edible seeds Such as hemp seeds, and not limited to almonds, coconuts, peanuts and chia seeds and flax seeds. cashews) and nuts such as almond, beech, brazil nut, 0170 According to another embodiment, the invention candlenut, cashew, chestnuts (including and not limited relates to any one of uses defined hereinabove, wherein the to Chinese chestnut or Sweet chestnut), coconut, colo pathogens selected from the group consisting of viruses, cynth, Cucurbita ficifolia, filbert, Gevulina avellana, bacteria, fungi, yeasts and moulds. hickory (including and not limited to pecan or shagbark 0171 According to another embodiment, the invention hickory), Terminalia catappa, hazelnut, Indian beech, relates to the third use defined hereinabove, wherein the kola nut, macadamia, Malabar chestnut, pistacia, mam pathogens selected from the group consisting of viruses, oncillo, maya nut, mongongo, oak acorns, ogbono nut, bacteria, fungi, yeasts and moulds, and further for the paradise nut, pili nut, walnut (including and not limited prevention of mucilage from seeds. to black walnut) or water caltrop: 0172 According to another embodiment, the invention 0.179 nut-like gymnosperm seeds such as pine nuts are relates to any one of uses defined hereinabove, wherein gymnosperm seeds that are edible (including and not pathogens are bacteria, preferably bacteria are selected from limited to cycads, ginkgo, Gnetum gnemon, juniper, the group consisting of E. Coli, Listeria monocytogenes and monkey-puzzle, pine nuts, or podocarps): Salmonella spp., and more preferably E. coli. 0180 other seeds such as cempedak, coffee, egusi, 0173 According to another embodiment, the invention euryale feroX (fox nut), fluted pumpkin, hemp seed, relates to any one of uses defined hereinabove, wherein jackfruit, lotus seed, Malabar gourd, pumpkin seed, pathogens cause disease in crop plants; said pathogens are Sunflower seed or sesame seed; US 2016/0295860 A1 Oct. 13, 2016 10

0181 beans Such as bambara groundnut, chickpeas, with the seeds and grains for 2 minutes to 48 hours and cowpeas (including and not limited to black eyed pea), preferably 24 hours before drying. dry beans, fava or broad beans, hyacinth bean, lablab. 0189 According to another embodiment, the invention lentils, lupins, Moringa oleifera, peas, peanuts, pigeon relates to any one of uses defined hereinabove, wherein peas, Sterculia, Velvet beans, winged beans, yam beans seeds are dried after contact with the synergistic composi or soybeans; tion and contact time, to reduce the moisture content of the 0182 seeds for sprouting, including, but not limited to, seeds below 10% and further contribute to prevent a pulses or legumes (such as, but not limited to, alfalfa, regrowth of microorganisms. clover, fenugreek, lentil, pea, chickpea, mung bean and 0190. According to another embodiment of the invention Soybean; cereals (such as, but not limited to, oat, wheat, relates to the use defined hereinabove, wherein said aqueous, maize (corn), rice, barley, rye, kamut, quinoa, amaranth synergistic composition is for an application to the seeds by and buckwheat, oilseeds (such as, but not limited to, spraying, Vaporizing, soaking, fumigating, or electrostatic Sesame, Sunflower, almond, hazelnut, linseed, peanut); spraying, preferably spraying. Of course, said spraying, brassicas or crucifers or cabbage family (such as, but Vaporizing, soaking, fumigating or electrostatic spraying can not limited to, broccoli, cabbage, watercress, mustard, be carried out according to any conventional technics well miZuna, radish, daikon (kaiware), rocket (arugula), known to the person skilled in the art. tatsoi and turnip); umbelliferous vegetables or parsley 0191). According to another embodiment, the composi family (such as, but not limited to, carrot, celery, tion of water-soluble ingredients (CWSI) or the powdered fennel, parsley; alliums or onion family (such as, but composition of water-soluble ingredients (PCWSI), may not limited to, onion, leek, green onion or me-negi); further comprises a plant defence enhancer selected from the other vegetables and herbs (such as, but not limited to, group consisting of at least one pesticide comprising a water spinach, lettuce, milk thistle and lemon grass); soluble silicate salt, at least one biopesticide comprising a 0183 seed spices including, but not limited to awain water soluble silicate salt , acilbenzolar-S-methyl, silica/ or carom seeds, alligator pepper or mbongo spice or silicate, DL-C.-amino-n-butyric acid (AABA), DL-B-amino mbongochobi or hepper pepper, allspice, anise, aniseed n-butyric acid (BABA), Y-amino-n-butyric acid (GABA), myrtle, annatto, borage, black cardamom, black mus p-aminobenzoic acid (PABA), riboflavin, salicylic acid tard, blue fenugreek or blue melilot, brown mustard, (SA), and Harpin protein (messenger). caraway, cardamom, celery seed, clove, coriander seed, 0.192 According to another embodiment, the composi cumin, dill seed, fennel, fenugreek, grains of paradise, tion of water-soluble ingredients (CWSI) or the powdered grains of Selim or Kani pepper, juniper berry, kala composition of water-soluble ingredients (PCWSI), may Zeera or kala jira or black cumin, kawakawa seeds, further comprises a sequestering agent, preferably in keluak or kluwak or kepayang, kokam seed, korarima amounts ranging from 0.01 to 10% w/w, relative to the total or Ethiopian cardamom or false cardamom, mace, weight of said composition. mahlab or Saint Lucie cherry, black mustard seed, brown mustard seed, white mustard seed, yellow mus 0193 According to another embodiment, the invention tard seed, nigella or kalonji or black caraway or black relates to the powdered composition defined hereinabove, onion seed, njangsa or dansang, nutmeg, black pepper wherein the acetylating agent is an organic acid containing seed, green pepper seed, black pepper seed, white at least one acyl group which is susceptible to perhydrolysis. pepper seed, star anise, Sumac, Szechuan pepper or 0194 According to another embodiment, the invention Sichuan pepper, Vanilla, wattleseed; relates to the powdered composition defined hereinabove, 0.184 seeds of crops transplantable from greenhouse to wherein the acetylating agent is a N-acyl compound or a field, including but not limited to basil, bell pepper, O-acyl compound containing an acyl radical R'—CO— broccoflower, broccoli, brussels sprouts, cabbage, can wherein R' is an aliphatic group having from 5 to 18 carbon taloupe, cauliflower, celery, cucumber, eggplant, head atoms, or an alkylaryl group having from 11 to 24 carbon atoms, with 5 to 18 carbon atoms in the alkyl chain. lettuce, honeydew, muskmelon, onion, radicchio, Preferably, Fe is an aliphatic group having from 5 to 12 romaine lettuce, squash, tobacco, tomato, watermelon; carbon atoms. O 0.195 According to another embodiment, the invention 0185 medical marijuana. relates to the wetting agent is Bio-Terge(R). AS-90 (a surfac 0186. According to another embodiment, the invention tant) consisting of an alpha olefin Sulfonate having from 12 relates to any one of uses defined hereinabove, wherein the to 18 carbon atoms. seeds are seeds of whole dried pear, split pea dried, pea fiber, 0196. For example, the powdered composition of water oregano, Vanilla, basil, chia, Sesame, sprouted flax and chia, soluble ingredients defined hereinabove, can be mixed with flax and hemp, and more preferably hemp seeds, flax seeds water at a concentration of 0.4 to 0.6% (4 to 6 Kg of formula and chia seeds. diluted in potable water (i.e. 40L) in order to sanitize 1 ton 0187. According to another embodiment, the invention of edible seeds (i.e. hemp seeds). To do so, according to a relates to any one of uses defined hereinabove, wherein said particularly preferred aspect of the invention, first the above aqueous, Synergistic composition is for an application to the mentioned the powdered composition of water-soluble Seeds by spraying, vaporizing, soaking, fumigating, or elec ingredients was dissolved in water and mixed for at least 10 trostatic spraying, preferably spraying. minutes, and then the solution obtained was applied to the 0188 According to another embodiment, the invention seeds to be treated. Then seeds were kept in contact with the relates to any one of uses defined hereinabove, wherein said solution for 2 to 5 minutes, and preferably then rinsed synergistic composition is for an application to the seeds by thoroughly with abundant potable water and finally the seeds spraying and keeping the Synergistic composition in contact were dried very well. US 2016/0295860 A1 Oct. 13, 2016

0197) It is to be noted that according to another preferred acetic acid and hydrogen peroxide in-situ. It is a non embodiment of the invention, a contact time of 2 minutes foaming solution and free of Surface-active agents. This reveals to be sufficient for reducing the total population of Powdered PAA is equivalent to 10% peracetic acid. bacteria present on seeds surface to below 1 million CFU per gram and reduce the E. coli, coliforms, yeast and mould to Preparation: undetectable levels. 0198 Alternatively, in the case some edible seeds such as (0209 100 g of Powdered PAA was dissolved in 1 L water flax, cannot tolerate water, other types of spraying with no and mixed for 10-15 min until peracetic acid is generated added water can be used. In such case, for example fumi in-situ. Both peracetic acid and hydrogen peroxide can be gation and electrostatic spraying may be preferred. Accord tested via Lamotte test kit code 7191-02. This solution was ing to an embodiment of the invention, fogging and elec to be used within 6 hours to maintain a high concentration trostatic spraying are preferred. Indeed, fogging and of peracetic acid. electrostatic spraying reduce the water particle size to pro duce smaller droplets that do not alter the external shell of 4. Neo Pure Preparation: the seed. Of course, said spraying, vaporizing, soaking, fumigating or electrostatic spraying can be carried out Description: according to any conventional technics well known to the 0210 Neo Pure is a powdered composition that generates person skilled in the art. peracetic acid in-situ via TAED, sodium percarbonate and citric acid mixture. Also, it generates hydrogen peroxide and BRIEF DESCRIPTION OF THE DRAWINGS contains a poylglycoside wetting agent (i.e. a surfactant). (0199 The present invention will be better understood Neo Pure is equivalent to 10% peracetic acid. More particu with reference to the following preferred drawing illustrat larly, the Neo Pure had the following formulation: ing particularly preferred and non-limitative embodiments of the invention. Sodium percarbonate 58% Wiw 0200 FIGS. 1 to 3 represent results obtained according to Tetraacetylethylenediamine (TAED) 20% wiw Example 2, and Citric acid (food-grade) 18% Wiw 0201 FIGS. 4 to 6 represent results obtained according to Glucooon 50 G surfactant (alkylpolyglycoside) 4% Wiw Example 3. (0202 More particularly preferred embodiments of the Total 100% invention will be illustrated in the following examples. EXAMPLE ii.1 Preparation: Protocols for Seeds, Grains and Spices Sanitation 0211 100 g of Neo Pure was dissolved in 1 L water and mixed for 10-15 min until peracetic acid was generated I—Preparation of Different Solutions in-situ. Both peracetic acid and hydrogen peroxide can be 0203 1. Wetting Agent (i.e. Surfactant) Preparation: tested via Lamotte test kit code 7191-02. This solution was to be used within 6 hours to maintain a high concentration Description: of peracetic acid. 0204 APG(R) 325 is a liquid wetting agent (i.e. a surfac 5. Neo Pure Preparation with Alcohol: tant) composed of alkyl polyglycoside and derived from Description: natural sources. It is a foaming Surfactant. 0212 Neo Pure is a powdered composition that generates Preparation: peracetic acid in-situ via TAED, sodium percarbonate and 0205 5g of liquid APG 325 surfactant were diluted in 1 citric acid mixture. Also it generates hydrogen peroxide and L water, and mixed for 5 minutes, to make 0.5% solution of contains a poylglycoside wetting agent (i.e. a surfactant). wetting agent (i.e. Surfactant). Neo Pure is equivalent to 10% peracetic acid. 2. Alcohol Preparation: Preparation: Description: 0213 100 g of Neo Pure were dissolved in 1 L water and mixed for 10-15 min until peracetic acid was generated 0206. A food-grade alcohol based on ethanol at 94% in-situ. Then, 100 ml of the solution so obtained was mixed concentration minimum (provided by Greenfield Ethanol). with 100 ml ethanol 94% for 10 minutes. More particularly, Preparation: the Neo Pure had the following formulation: 0207 100 ml of the above-mentioned ethanol were 6. Liquid Peracetic Acid Preparation Without Wetting Agent: diluted in 100 ml water to a make a 50% food grade ethanol. 3. Powdered Peracetic Acid Preparation without Surfactant: Description: Description: 0214) PERCID is a CFIA approved liquid preformed peracetic acid. PERCID is a concentrated 5% peracetic acid 0208. Ablend of sodium percarbonate (62% w/w), TAED formula composed of mixing liquid acetic acid with liquid (20% w/w) and citric acid (18% w/w) that generates per hydrogen peroxide. US 2016/0295860 A1 Oct. 13, 2016

Preparation: Preparation: 0215 200 ml of PERCID was dissolved in 1 L water and 0221 100 g of Powdered PAA were dissolved in 1 L mixed for 5 minutes. A non-foaming solution free of Surface water, and mixed for 10-15 min until peracetic acid is active agents such as a wetting agent, was obtained. generated in-situ. Then, 100 ml of the solution so obtained 7. Liquid Peracetic Acid Preparation with a Wetting Agent was mixed with 100 ml ethanol 94% for 10 minutes. (i.e. a Surfactant): 10. Liquid Peracetic Acid Preparation with Alcohol: Description: Description: 0216. PERCID is a CFIA approved liquid preformed 0222 PERCID is a CFIA approved liquid preformed peracetic acid. Percid is a concentrated 5% peracetic acid peracetic acid. PERCID is a concentrated 5% peracetic acid formula composed of mixing liquid acetic acid with liquid formula composed of mixing liquid acetic acid with liquid hydrogen peroxide. PERCID solution is mixed a liquid hydrogen peroxide. wetting agent (i.e. surfactant) APG 325. Preparation: Preparation: 0223 200 ml of PERCID were dissolved in 1 L water, 0217 200 ml of PERCID was dissolved in 1 L water and and mixed for 5 minutes. A non-foaming PAA Solution was mixed for 5 minutes. Then, 5 g of APG(R) 325 was added to obtained. Then, 100 ml of this non-foaming PAA was mixed the solution so obtained, and mixed for 5 minutes. A foaming with 100 ml Ethanol 94% for 10 minutes, to provide the PAA solution was obtained. liquid peracetic acid preparation with alcohol. 8. Liquid Peracetic Acid Preparation with Wetting Agent (i.e. Surfactant) and Alcohol: II—Preparation of Different Seeds Grains. Description: 0224 Seeds were mechanically cleaned and spread in stainless steel containers. Each 100 grams seeds were 0218 PERCID is a CFIA approved liquid preformed sprayed with 4 ml total solutions descried above via con peracetic acid. PERCID is a concentrated 5% peracetic acid ventional trigger vaporizer. This solution is equivalent to 40 formula composed of mixing liquid acetic acid with liquid L disinfecting solution total sprayed on 1-ton seeds. Seeds, hydrogen peroxide. PERCID solution is mixed a liquid grains and spices were selected to represent all families and wetting agent (i.e. surfactant) APG(R) 325. types of seeds, grains and spices. Another criteria was to Preparation: select seeds and grains contaminated with a high count of total aerobic bacteria, yeast, mold, E. coli, Salmonella sp. 0219, 200 ml of PERCID was dissolved in 1 L water, and and other pathogenic microorganisms. mixed for 5 minutes. Then, 5g of APG(R) 325 were added to 0225 Seeds, grains and spices treated were: the resulting Solution, and mixed for 5 minutes. A foaming 0226) Whole dried pea, PAA solution was obtained. Then, 100 ml of this foaming 0227 Split pea dried, PAA was mixed with 100 ml ethanol 94% for 10 minutes, to 0228 Pea fiber, provide the liquid peracetic acid preparation with wetting 0229 Vanilla, agent and alcohol. 0230 Chia, 9. Powdered Peracetic Acid Preparation with Alcohol: 0231 Sprouted flax and chia, Description: 0232 Flax, 0233 Hemp, and 0220. A blend of sodium percarbonate (62%), TAED (20%) and citric acid (18%) that generates peracetic acid and 0234 Black pepper seeds hydrogen peroxide in-situ. It is a non-foaming Solution and free of surface active agents. This powdered PAA is equiva III RESULTS on Whole Dried Pea lent to 10% peracetic acid. 0235

Total count Aerobic - Physical Treatments CFUg bacteria Characteristics Organoleptic O- Untreated Av = 800,000 No effect No effect n1 = 700,000 n2 = 890,000 Av = 1,000 Y&M n1 = 1000m2 = 1000 1- Wetting agent APG 325 Av = 925,000 No effect No effect n1 = 850,000 n2 = 1 million Av = 1,000 Y&M n1 = 1000m2 = 1000 2- Alcohol - ethanol Av = 900,000 No effect No effect n1 = 800,000 n2 = 1 million Y&M not detected n1, n2 not detected US 2016/0295860 A1 Oct. 13, 2016 13

-continued

Total count Aerobic - Physical Treatments CFUg bacteria Characteristics Organoleptic 3- Powdered PAA Alone Av = 300,000 No effect No effect 4 kg 40 L n1 = 250,000 n2 = 350,000 Y&M not detected n1, n2 not detected 4- Neo Pure A.y = 150,000 No effect No effect 4 kg 40 L n1 = 170,000 n2 = 130,000 Y&M not detected n1, n2 not detected 5- Neo Pure + alcohol Av = 60,000 No effect No effect 2 kg/20 L + 2.0 L n1 = 60,000 n2 = 60,000 Y&M not detected n1, n2 not detected 6- Percid -Liquid PAA alone Av = 250,000 No effect No effect 8 Lt AOL n1 = 230,000 n2 = 270,000 Y&M not detected n1, n2 not detected 7- Percid -Liquid PAA Av = 50,000 No effect No effect 8 L40 L + wetting agent n1 = 80,000 n2 = 20,000 Y&M not detected n1, n2 not detected 8- Percid -Liquid PAA + Av = 5,000 No effect No effect wetting agent + alcohol (94%) n1 = 5,000 4 Li 20 L + 2.0 L ethanol + n2 = 5,000 wetting agent Y&M not detected n1, n2 not detected 9- Powdered PAA + Av = 70,000 No effect No effect alcohol (94%) n1 = 85,000 2 kg/20 L + 2.0 L ethanol n2 = 55,000 Y&M not detected n1, n2 not detected 10- Percid -Liquid PAA + Av = 60,000 No effect No effect alcohol n1 = 80,000 4 Li 20 L + 2.0 L ethanol n2 = 40,000 (94%) Y&M not detected n1, n2 not detected

0236 Conclusion: 0241 Alcohol (ethanol) combined to peracetic acid 0237) The polyglycoside wetting agent (i.e. Surfactant) formulations increased the efficiency of the oxidizer was not bactericidal and can act as a food source for the and showed to be synergistic with peracetic acid. bacteria 0238 Alcohol (50% concentration) applied at ratio of 0242 Both alcohol and wetting agent (i.e. surfactant) 40 L per 1 ton peas was not a strong bactericidal agent. increase the coverage of peracetic acid and help this 0239) Peracetic acid either preformed via liquid for limited amount of solution (40 L per 1 ton seed) to mulations or in-situ generated via powdered formula cover better the seeds and penetrate the seeds and target tions, showed to be a strong bactericidal agent and microorganisms. They showed a synergistic effect that reduced the level of bacteria, yeast and mold signifi is higher that the one of the peracetic acid with a cantly. wetting agent or the peracetic acid with an alcohol. 0240 Wetting agent (i.e. Surfactant) combined to per acetic acid formulations increased the efficiency of the IV Results on Whole Split Pea oxidizer and showed to be synergistic with peracetic acid. 0243

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic

O- Untreated Av = 800,000 No effect No effect n1 = 750,000 n2 = 850,000 Av = 1,000 Y&M n1 = 1000m2 = 1000 US 2016/0295860 A1 Oct. 13, 2016 14

-continued

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic 1- Wetting agent APG 325 Av = 900,000 No effect No effect O.S9/o n1 = 930,000 n2 = 870,000 Av = 1,000 Y&M n1 = 1000m2 = 1000 2- Alcohol - ethanol Av = 700,000 No effect No effect SO% n1 = 700,000 n2 = 700,000 Y&M not detected n1, n2 not detected 3- Powdered PAA Alone Av = 400,000 No effect No effect 4 kg 40 L n1 = 430,000 n2 = 370,000 Y&M not detected n1, n2 not detected 4- Neo Pure Av = 350,000 No effect No effect 4 kg 40 L n1 = 450,000 n2 = 250,000 Y&M not detected n1, n2 not detected 5- Neo Pure + alcohol Av = 100,000 No effect No effect 2 kg/20 L + 2.0 L ethanol (94%) n1 = 80,000 n2 = 120,000 Y&M not detected n1, n2 not detected 6- Percid -Liquid PAA alone Av = 300,000 No effect No effect 8 Lt AOL n1 = 400,000 n2 = 200,000 Y&M not detected n1, n2 not detected 7- Percid -Liquid PAA Av = 200,000 No effect No effect 8 L40 L + wetting agent n1 = 200,000 n2 = 200,000 Y&M not detected n1, n2 not detected 8- Percid -Liquid PAA + Av = 50,000 No effect No effect wetting agent + alcohol (94%) n1 = 80,000 4 Li 20 L + 2.0 L ethanol + n2 = 30,000 wetting agent Y&M not detected n1, n2 not detected 9- Powdered PAA+ Av = 150,000 No effect No effect alcohol (94%) n1 = 200,000 2 kg/20 L + 2.0 L ethanol n2 = 100,000 Y&M not detected n1, n2 not detected 10- Percid -Liquid PAA + Av = 150,000 No effect No effect alcohol 4 L20 L + n1 = 130,000 20 Lethanol (94%) n2 = 170,000 Y&M not detected n1, n2 not detected

0244 Conclusion: 0248 Wetting agent (i.e. surfactant) combined to per acetic acid formulations increased the efficiency of the 0245 The polyglycoside wetting agent (i.e. Surfactant) oxidizer and showed to be synergistic with peracetic was not bactericidal and can act as a food source for the acid. bacteria 0249 Alcohol (ethanol) combined to peracetic acid formulations increased the efficiency of the oxidizer 0246 Alcohol (50% concentration) applied at ratio of and thus showed to be synergistic with peracetic acid. 40 L per 1 ton peas was not a strong bactericidal agent 0250) Both alcohol and wetting agent (i.e. surfactant) on split pea. increased the coverage of peracetic acid and helped this limited amount of solution (40L per 1 ton seed) to cover 0247 Peracetic acid either preformed via liquid for better the seeds and penetrate the seeds and target mulations or in-situ generated via powdered formula microorganisms. They showed a synergistic effect that tions, was a strong bactericidal agent and reduce the is higher that the one of the peracetic acid with a level of bacteria, yeast and mold significantly. wetting agent or the peracetic acid with an alcohol. US 2016/0295860 A1 Oct. 13, 2016 15

V Results on Pea Fiber 0251

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic O- Untreated Av = 900,000 No effect No effect n1 = 700,000 n2 = 1.1 million Av = 1,000 Y&M n1 = 1000m2 = 1000 1- Surfactant APG 325 Av = 900,000 No effect No effect O.S9/o n1 = 850,000 n2 = 950,000 Av = 1,000 Y&M n1 = 1000m2 = 1000 2- Alcohol - ethanol Av = 700,000 No effect No effect SO% n1 = 500,000 n2 = 900,000 Y&M not detected n1, n2 not detected 3- Powdered PAA Alone Av = 500,000 No effect No effect 4 kg 40 L n1 = 700,000 n2 = 300,000 Y&M not detected n1, n2 not detected 4- Neo Pure Av = 500,000 No effect No effect 4 kg 40 L n1 = 450,000 n2 = 550,000 Y&M not detected n1, n2 not detected 5- Neo Pure + alcohol Av = 250,000 No effect No effect 2 kg/20 L + 2.0 L ethanol (94%) n1 = 200,000 n2 = 300,000 Y&M not detected n1, n2 not detected 6- Percid -Liquid PAA alone Av = 600,000 No effect No effect 8 Lt AOL n1 = 600,000 n2 = 600,000 Y&M not detected n1, n2 not detected 7- Percid -Liquid PAA Av = 400,000 No effect No effect 8 L40 L + surfactant n1 = 500,000 n2 = 300,000 Y&M not detected n1, n2 not detected 8- Percid -Liquid PAA + Av = 300,000 No effect No effect surfactant + alcohol (94%) n1 = 330,000 4 Li 20 L + 2.0 L ethanol + n2 = 270,000 Surfactant Y&M not detected n1, n2 not detected 9- Powdered PAA+ Av = 300,000 No effect No effect alcohol (94%) n1 = 400,000 2 kg/20 L + 2.0 L ethanol n2 = 200,000 Y&M not detected n1, n2 not detected 10- Percid -Liquid PAA + Av = 450,000 No effect No effect alcohol 4 L20 L + n1 = 600,000 20 Lethanol (94%) n2 = 300,000 Y&M not detected n1, n2 not detected

0252 Conclusion: 0256 Peracetic acid either preformed via liquid for (0253) The solution affected the fiber pea size due to mulations or in-situ generated via powdered formula humidity. However, drying can restore the size of fiber tions, was a strong bactericidal agent and reduced the pea as the untreated. level of bacteria, yeast and mold significantly. 0254 The polyglycoside wetting agent (i.e. Surfactant) 0257 Wetting agent (i.e. surfactant) combined to per was not bactericidal and can act as a food source for the acetic acid formulation increased the efficiency of the bacteria oxidizer and thus showed to be synergistic with per 0255 Alcohol (50% concentration) applied at ratio of acetic acid. 40 L per 1 ton peas was not a strong bactericidal agent 0258 Alcohol (ethanol) combined to peracetic acid on pea fiber at used concentration (i.e. 40 L of alcohol formulations increases the efficiency of the oxidizer 50% active per 1 ton). and thus showed to be synergistic with peracetic acid. US 2016/0295860 A1 Oct. 13, 2016 16

0259 Both alcohol and wetting agent (i.e. surfactant) 0263. The polyglycoside wetting agent (i.e. Surfactant) increased the coverage of peracetic acid and helped this was not bactericidal and can act as a food source for the limited amount of solution (40 L per 1 ton seed) to bacteria cover better the seeds and penetrate the seeds and target 0264 Alcohol (50% concentration) applied at ratio of microorganisms. They showed a synergistic effect that 40 L per 1 ton Vanilla was not a strong bactericidal is higher that the one of the peracetic acid with a agent on Vanilla. wetting agent or the peracetic acid with an alcohol. 0265 Peracetic acid either preformed via liquid for mulations or in-situ generated via powdered formula VI—Results on Vanilla tions, was a strong bactericidal agent and reduced the 0260 level of bacteria, yeast and mold significantly.

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic O- Untreated Av = 2 millions No effect No effect n1 = 1.5 millions n2 = 2.5 millions Av = 1,000 Y&M n1 = 1000m2 = 1000 1- Wetting agent APG 325 Av = 2 millions No effect No effect O.S9/o n1 = 1.3 millions n2 = 2.7 millions Av = 1,000 Y&M n1 = 1000m2 = 1000 2- Alcohol - ethanol Av = 1 million No effect No effect SO% n1 = 1 million n2 = 1 million Av = 1,000 Y&M n1 = 1000m2 = 1000 3- Powdered PAA Alone Av = 900,000 No effect No effect 4 kg 40 L n1 = 600,000 n2 = 1.2 millions Y&M not detected n1, n2 not detected 4- Neo Pure Av = 500,000 No effect No effect 4 kg 40 L n1 = 600,000 n2 = 400,000 Y&M not detected n1, n2 not detected 5- Neo Pure + alcohol Av = 200,000 No effect No effect 2 kg/20 L + 2.0 L ethanol (94%) n1 = 150,000 n2 = 250,000 Y&M not detected n1, n2 not detected 6- Percid -Liquid PAA alone Av = 500,000 No effect No effect 8 Lt AOL n1 = 600,000 n2 = 400,000 Y&M not detected n1, n2 not detected 7- Percid -Liquid PAA Av = 300,000 No effect No effect 8 L40 L + wetting agent n1 = 320,000 n2 = 280,000 Y&M not detected n1, n2 not detected 8- Percid -Liquid PAA + Av = 300,000 No effect No effect wetting agent + alcohol (94%) n1 = 310,000 4 Li 20 L + 2.0 L ethanol + n2 = 290,000 wetting agent Y&M not detected n1, n2 not detected 9- Powdered PAA+ Av = 300,000 No effect No effect alcohol (94%) n1 = 300,000 2 kg/20 L + 2.0 L ethanol n2 = 300,000 Y&M not detected n1, n2 not detected 10- Percid -Liquid PAA + Av = 300,000 No effect No effect alcohol 4 L20 L + n1 = 330,000 20 Lethanol (94%) n2 = 270,000 Y&M not detected n1, n2 not detected

0261 Conclusion: 0266 Wetting agent (i.e. surfactant) combined to per 0262 The solutions were sprayed on Vanilla as acetic acid formulations increased the efficiency of the received in rod shaped to reduced the level of total oxidizer and thus showed to be synergistic with per aerobic count. acetic acid. US 2016/0295860 A1 Oct. 13, 2016 17

0267 Alcohol (ethanol) combined to peracetic acid microorganisms. They showed a synergistic effect that formulations increased the efficiency of the oxidizer is higher that the one of the peracetic acid with a and thus showed to be synergistic with peracetic acid. wetting agent or the peracetic acid with an alcohol. 0268. Both alcohol and wetting agent (i.e. surfactant) increased the coverage of peracetic acid and helped this VII—Results on Chia Seeds limited amount of solution (40 L per 1 ton seed) to cover better the seeds and penetrate the seeds and target 0269

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic O- Untreated Av = 1 million Mucilage No effect n1 = 1.1 million observed n2 = 900,000 Av = 10,000 Y&M n1 = 10,000/n2 = 10,000 1- Wetting agent APG 325 Not tested due to Mucilage Mucilage mucilage observed observed 2- Alcohol - ethanol Av = 510,000 No mucilage No mucilage n1 = 520,000 n2 = 500,000 Av = 10,000 Y&M n1 = 10,000/n2 = 10,000 3- Powdered PAA Alone Not tested due to Mucilage Mucilage 4 kg 40 L mucilage observed observed 4- Neo Pure Not tested due to Mucilage Mucilage 4 kg 40 L mucilage observed observed 5- Neo Pure + alcohol Av = 300,000 No mucilage No effect. 2 kg/20 L + 2.0 L ethanol (94%) n1 = 330,000 n2 = 270,000 Av = 1,000 Y&M n1 = 1,000/n2 = 1,000 6- Percid -Liquid PAA alone Not tested due to Mucilage Mucilage mucilage observed observed 7- Percid -Liquid PAA Not tested due to Mucilage Mucilage 8 L40 L + wetting agent mucilage observed observed 8- Percid -Liquid PAA + Av = 300,000 No mucilage No effect. wetting agent + alcohol (94%) n1 = 350,000 4 Li 20 L + 2.0 L ethanol + n2 = 250,000 Surfactant Av = 1,000 Y&M n1 = 1,000/n2 = 1,000 9- Powdered PAA + Av = 400,000 No mucilage No effect. alcohol (94%) n1 = 420,000 2 kg/20 L + 2.0 L ethanol n2 = 380,000 Av = 1,000 Y&M n1 = 1,000/n2 = 1,000 10- Percid -Liquid PAA + Av = 200,000 No mucilage No effect. alcohol 4 L20 L + n1 = 150,000 20 Lethanol (94%) n2 = 250,000 Y&M not detected n1, n2 not detected

0270 Conclusion: 0271 The presence of alcohol prevents the release of mucilage. 0272. The PAA in 50% Alcohol seems efficacious in reducing bacteria and yeast. VIII Results on Flax Seeds 0273

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic

O- Untreated Av = 5 millions Mucilage No effect n1 = 6.5 millions observed n2 = 3.5 millions Av = 1,000 Y&M n1 = 1,000/n2 = 1,000 US 2016/0295860 A1 Oct. 13, 2016 18

-continued

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic 1- Wetting agent APG 325 Not tested due to Mucilage Mucilage O.S9/o mucilage observed observed 2- Alcohol - ethanol Av = 2 millions No mucilage No mucilage SO% n1 = 1.8 millions n2 = 2.2 millions Av = 1,000 Y&M n1 = 1,000/n2 = 1,000 3- Powdered PAA Alone Not tested due to Mucilage Mucilage 4 kg 40 L mucilage observed observed 4- Neo Pure Not tested due to Mucilage Mucilage 4 kg 40 L mucilage observed observed 5- Neo Pure + alcohol Av = 1 million No mucilage No effect. 2 kg/20 L + 2.0 L ethanol (94%) n1 = 1 million n2 = 1 million Y&M not detected n1, n2 not detected 6- Percid -Liquid PAA alone Not tested due to Mucilage Mucilage 8 Lt AOL mucilage observed observed 7- Percid -Liquid PAA Not tested due to Mucilage Mucilage 8 L40 L + wetting agentt mucilage observed observed 8- Percid -Liquid PAA + Av = 700,000 No mucilage No effect. wetting agent + alcohol (94%) n1 = 850,000 4 Li 20 L + 2.0 L ethanol + n2 = 550,000 Surfactant Y&M not detected n1, n2 not detected 9- Powdered PAA+ alcohol (94%) Av = 900,000 No mucilage No effect. 2 kg/20 L + 2.0 L ethanol n1 = 915,000 n2 = 885,000 Y&M not detected n1, n2 not detected 10- Percid -Liquid PAA + alcohol Av = 800,000 No mucilage No effect. 4 L/20 L + 2.0 L ethanol (94%) n1 = 800,000 n2 = 800,000 Y&M not detected n1, n2 not detected

0274 Conclusion: (0277. The PAA in 50%. Alcohol showed to be effica 0275. The presence of alcohol showed to prevent the cious in reducing bacteria and yeast. release of mucilage. 0276 Macroscopically, mucilage was not observed IX—Results on Sprouted Flax and Chia with seeds treated with alcohol. 0278

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic

O- Untreated Av = 2 millions Mucilage No effect n1 = 2.5 millions observed, n2 = 1.5 millions very wet Av = 1,000 Y&M n1 = 1,000/n2 = 1,000 1- Wetting agent APG 325 Not tested due to Mucilage Mucilage O.S9/o mucilage observed observed 2- Alcohol - ethanol Av = 600,000 No mucilage No mucilage n1 = 550,000 n2 = 650,000 Av = 1,000 Y&M n1 = 1,000/n2 = 1,000 3- Powdered PAA Alone Not tested due to Mucilage Mucilage 4 kg 40 L mucilage observed observed 4- Neo Pure Not tested due to Mucilage Mucilage 4 kg 40 L mucilage observed observed 5- Neo Pure + alcohol Av = 200,000 No mucilage No effect. 2 kg/20 L + 2.0 L ethanol (94%) n1 = 220,000 n2 = 180,000 Y&M not detected n1, n2 not detected US 2016/0295860 A1 Oct. 13, 2016 19

-continued

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic 6- Percid -Liquid PAA alone Not tested due to Mucilage Mucilage 8 Lt AOL mucilage observed observed 7- Percid -Liquid PAA Not tested due to Mucilage Mucilage 8 L40 L + wetting agent mucilage observed observed 8- Percid -Liquid PAA + Av = 500,000 No mucilage No effect. wetting agent + alcohol (94%) n1 = 525,000 4 Li 20 L + 2.0 L ethanol + n2 = 475,000 wetting agent Y&M not detected n1, n2 not detected 9- Powdered PAA + alcohol (94%) Av = 600,000 No mucilage No effect. 2 kg/20 L + 2.0 L ethanol n1 = 600,000 n2 = 600,000 Y&M not detected n1, n2 not detected 10- Percid -Liquid PAA + alcohol Av = 600,000 No mucilage No effect. 4 L/20 L + 2.0 L ethanol (94%) n1 = 500,000 n2 = 700,000 Y&M not detected n1, n2 not detected

0279 Conclusion: 0283 The PAA in 50%. Alcohol showed to be effica 0280 Humidity including alcohol solution may affect cious in reducing bacteria and yeast. the sprouted flax and chia. It should be dried well. 0284 Macroscopically, mucilage was not observed (0281 The presence of alcohol showed to prevent the with seeds treated with alcohol. release of mucilage 0282 Alcohol showed to act as a bactericidal agent but X—Results on Hemp not very strong. 0285

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic O- Untreated Av = 2 millions No effect No effect n1 = 1.7 millions n2 = 2.3 millions Av = 1,000 Y&M n1 = 1000m2 = 1000 1- Wetting agent APG 325 Av = 2 millions No effect No effect n1 = 1.5 millions n2 = 2.5 millions Av = 1,000 Y&M n1 = 1000m2 = 1000 2- Alcohol - ethanol Av = 1 million No effect No effect n1 = 800,000 n2 = 1.2 millions Av = 1,000 Y&M n1 = 1000m2 = 1000 3- Powdered PAA Alone Av = 500,000 No effect No effect 4 kg 40 L n1 = 600,000 n2 = 400,000 Y&M not detected n1, n2 not detected 4- Neo Pure Av = 300,000 No effect No effect 4 kg 40 L n1 = 300,000 n2 = 300,000 Y&M not detected n1, n2 not detected 5- Neo Pure + alcohol Av = 100,000 No effect No effect 2 kg/20 L + 2.0 L ethanol (94%) n1 = 120,000 n2 = 80,000 Y&M not detected n1, n2 not detected 6- Percid -Liquid PAA alone A.y = 310,000 No effect No effect 8 Lt AOL n1 = 300,000 n2 = 320,000 Y&M not detected n1, n2 not detected US 2016/0295860 A1 Oct. 13, 2016 20

-continued

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic 7- Percid -Liquid PAA Av = 300,000 No effect No effect 8 L40 L + wetting agent n1 = 320,000 n2 = 280,000 Y&M not detected n1, n2 not detected 8- Percid -Liquid PAA + Av = 200,000 No effect No effect wetting agent + alcohol (94%) n1 = 100,000 4 Li 20 L + 2.0 L ethanol + n2 = 300,000 Surfactant Y&M not detected n1, n2 not detected 9- Powdered PAA+ alcohol (94%) Av = 200,000 No effect No effect 2 kg/20 L + 2.0 L ethanol n1 = 200,000 n2 = 200,000 Y&M not detected n1, n2 not detected 10- Percid -Liquid PAA + alcohol Av = 300,000 No effect No effect 4 L/20 L + 2.0 L ethanol (94%) n1 = 330,000 n2 = 270,000 Y&M not detected n1, n2 not detected

0286 Conclusion: oxidizer and thus showed to be synergistic with per 0287. The above-mentioned solutions were sprayed on acetic acid. hemp seeds to achieve a reduction of the level of total 0292 Alcohol (ethanol) combined to peracetic acid aerobic count. formulations increased the efficiency of the oxidizer 0288 The polyglycoside wetting agent (i.e. surfactant) and thus showed to be synergistic with peracetic acid. was not bactericidal. 0293 Both alcohol and surfactant increase the cover 0289 Alcohol (50% concentration) applied at ratio of age of peracetic acid and help this limited amount of 40 L per 1 ton hemp seed was not a strong bactericidal solution (40 L per 1 ton seed) to cover better the seeds agent on hemp. and penetrate the seeds and target microorganisms. 0290 Peracetic acid either preformed via liquid for They showed a synergistic effect that is higher that the mulations or in-situ generated via powdered formula one of the peracetic acid with a wetting agent or the tions, was a strong bactericidal agent and reduced the peracetic acid with an alcohol. level of bacteria, yeast and mold significantly. 0291 Wetting agent (i.e. surfactant) combined to per XI Results on Black Pepper acetic acid formulations increased the efficiency of the 0294

Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic

O- Untreated Av = 1.5 millions No effec No effec Av = 3,000 Y&M 1- Surfactant APG 325 Av = 1.7 millions No effec No effec O.S90 Av = 3,000 Y&M 2- Alcohol - ethanol Av = 1 million No effec No effec SO% Av = 1,000 Y&M 3- Powdered PAA Alone Av = 500,000 No effec No effec 4 kg/40 L Av = 100 Y&M 4- Neo Pure Av = 480,000 No effec No effec 4 kg/40 L Av = 100 Y&M 5- Neo Pure + alcohol Av = 370,000 No effec No effec 2 kg/20 L + 20 Lethanol (94%) Av = 100 Y&M 6- Percid -Liquid PAA alone Av = 460,000 No effec No effec 8 Lt AOL Y&M not detected 7- Percid -Liquid PAA Av = 400,000 No effec No effec 8 L40 L + surfactant Y&M not detected 8- Percid -Liquid PAA + Av = 380,000 No effec No effec surfactant + alcohol (94%) Y&M not detected 4 Li 20 L + 2.0 L ethanol + Surfactant 9- Powdered PAA+ Av = 400,000 No effec No effec alcohol (94%) Y&M not detected 2 kg/20 L + 2.0 L ethanol US 2016/0295860 A1 Oct. 13, 2016

-continued Total count Aerobic Physical Treatments CFUg bacteria Characteristics Organoleptic 10- Percid -Liquid PAA + Av = 380,000 No effect No effect alcohol 4 L20 L + Y&M not detected 20 Lethanol (94%)

0295 Conclusion: formula 18/18). Said formula 18/18 is powdered formulation 0296. The disinfecting solutions sprayed on black pep is based on 40% sodium percarbonate, mixed with 20% per seeds, can reduce the level of total aerobic count. TAED; mixed with 18% potassium silicate; mixed with 18% 0297. The polyglycoside surfactant is not bactericidal. EDTA acid; and finally mixed with 4% Bioterge AS 90 surfactant. The 4 Kg were diluted in 40 L water and were 0298 Alcohol (50% concentration) applied at ratio of mixed thoroughly for 10 minutes and then applied to treat 1 40 L per 1 ton Black pepper seed is not a strong ton of hemp seed for 30 minutes, then the treated seeds were bactericidal agent on hemp. dried very well as per the grain conditioner process. The 0299 Peracetic acid either preformed via liquid for results showed a reduction in total bacterial count to 54,000 mulations or in-situ generated via powdered formula CFU per gram. These results were satisfactory and complied tions, is a strong bactericidal agent and reduce the level with the market standards. Coliforms, E. coli, yeast and of bacteria, yeast and mold significantly. mould were not detected. 0300 Alcohol (ethanol) combined to peracetic acid formulations increases the efficiency of the oxidizer Conclusions: and thus is synergistic with peracetic acid. 0301 Both alcohol and surfactant increase the cover 0305 Based on the results shown above, there was noted age of peracetic acid and help this limited amount of a synergy between oxidizers (i.e. peracetic acid and hydro solution (40 L per 1 ton seed) to cover better the seeds gen peroxide) and wetting agent (i.e. Surfactant) in reducing and penetrate the seeds and target microorganisms. the populations of human pathogens on edible seeds. EXAMPLE 2 EXAMPLE 3 A Trial Assessing the Efficacy of Powdered Determination of Contact-Time Efficacy of Formula (Powdered PAA with a Wetting Agent) in Powdered PAA Combined with Alcohol and a the Surface Disinfection of Hemp Seeds in a Grain Wetting Agent Conditioning Facility (0306 Objective: The objective of this study is to deter mine the effective contact-time of the sanitizing solutions Protocol: (PAA with an alcohol and a wetting agent) sprayed on hemp 0302 Several tons of hemp seeds were cleaned mechani seeds in controlling pathogens. cally using regular grain conditioning equipment. The total 0307 Protocol: 1 kg of hemp seeds per mix was treated bacterial count was determined to be about 18 million with 50 ml of Solution by applying Small amounts at a time CFU/g (before mechanical cleaning and separation). After using a hand sprayer and mixing thoroughly in between. mechanical cleaning, the total bacterial count was found to Batches were stored in 3.3 L containers at room temperature be about 2 million CFU/g. This microbial load does not with lids on to avoid loss of moisture due to evaporation. comply with the market standard which is 1 million CFU/g. 0308 Solutions Used: Treatment with Peracetic Acid and Hydrogen Peroxide 0309 Neo-Pure (5%), (50 g Neo Pure dissolved in 1 L Without an Alcohol and/or a Wetting Agent: water and mixed for 15 minutes) 0303 Hemp seeds (with a microbial load of about 2 0310 Neo-Pure / Ethanol (5% Neo Pure dissolved in million CFU/g) were sanitized with a powdered product 50% water/50% alcohol), based on sodium percarbonate, TAED and citric acid that 0311 Mock (HO) generates peracetic acid and hydrogen peroxide in situ. An Samples were taken at the indicated time points and plated equivalent of 4 Kg of this formula were dissolved in potable immediately with the exception of the +1 hr time point in the water and mixed thoroughly for 10 minutes and then applied experiment of 8/25 (This sample was taken at +1 hr but to 1 ton of hemp seeds and allowed to remain in contact with stored at 4°C. of n and plated the next morning). them for 30 minutes. The seeds were thoroughly dried after the treatment. The results did not show a significant reduc Results tion in microbial load as compared to untreated seeds (2 million CFU/g). These results were not satisfactory. In 0312 addition, coliforms, E. coli, yeast and mould were detected. The powdered formulation that generates PAA in-situ was Batch treatment 2014 Aug. 25 based on 70% w/w sodium percarbonate, mixed with 20% Triplicates! w/w TAED and mixed with 10% citric acid. Treatment with Formula (Peracetic acid Generated In-Situ +1 hr +21 hrs +48 hrs +72 hrs with a Wetting Agent): UTC 786,000 3,200,000 620,000 890,000 0304 1 ton of cleaned hemp seeds (2 million CFU per mock 701,000 793,000 400,000 765,000 gram) were sanitized with a 0.4% concentration (4 kg of US 2016/0295860 A1 Oct. 13, 2016 22

-continued acetic acid precursor and consisting of a dry, mixture of: Batch treatment 2014 Aug. 25 (i)-a) 30-60% w/w of a solid hydrogen peroxide pre Triplicates! cursor, +1 hr +21 hrs +48 hrs +72 hrs (i)-b) 10-40% w/w of a pH adjusting agent which is an organic acid or an inorganic acid; and Neo-Pure 1,000,000 195,000 272,000 101,000 Neo-Pure EtOH 182,000 23,000 22,000 73,000 (i)-c) 10-40% w/w of an acetylating agent; and wherein said peracetic acid precursor generates in situ UTC means untreated and aforesaid data were reported on FIGS. 1 to 3. peracetic acid (PAA) when the powdered composition of water-soluble ingredients (PCWSI) is contacted with water (W) in a weight ratio (PCWSI)/(W) ranging from Batch treatment 2014 Sep. 10 1:100 to 1:4, being understood that when 2 g of said Pentuplicates dry, water-soluble mixture of (i)-a), (i)-b), (i)-c) is admixed with 1000 g of water, 100 to 250 ppm of starting point +3 hr on +24 hrs +48 hrs peracetic acid (PAA) are generated in situ at pH 9.0+3; UTC 12,700,000 and Neo- 17,700,000 8,400,000 2,660,000 4,520,000 Pure wherein (ii) said at least one agriculturally acceptable Neo- 2,400,000 1,600,000 1,600,000 1480,000 solvent is soluble in water (W) selected from the group Pure consisting of an agriculturally acceptable alcohol, gly EtOH col ether, propylene glycol and ethylene glycol, and represents from 2% by volume to 70% by volume of the UTC means untreated and aforesaid data were reported on FIGS. 4 to 6. total Volume of the Synergistic composition to be 0313 Conclusions: formed. 82. The composition of claim 81, wherein said at least one 0314 Under lab conditions (room temperature: 20° C. agriculturally acceptable solvent is an alcohol of formula to 27° C.) the efficacy of both Neo-Pure only and ROH where R represents a linear alkyl group having from 1 Neo-Pure--EtOH increased significantly with longer to 6 carbon atoms, or a branched alkyl group having from 3 incubation times (>24 hours) to 6 carbon atoms. 0315. This effect was much more prominent at the 83. The composition of claim 82, wherein said composi beginning of the treatment with Neo-Pure only. How tion of water-soluble ingredients further comprises: ever, it was noted that no significant regrowth of (iii) at least one wetting agent, wherein the at least one bacteria was observed within the first 48 hours post Wetting agent is: treatment if EtOH was present. an anionic Surfactant selected from the group consisting 0316. Once seeds were treated with PAA+alcohol and/ of carboxylates, Sulfonates, petroleum Sulfonates, alky or wetting agent, the sanitizing solution continues to Ibenzenesulfonates, naphthalene Sulfonates, olefin Sul work for hours and reduce the population of bacteria. fonates, alkyl sulfates, Sulfated natural oils, Sulfated However, after 48 hrs, seeds had to be dried to reduce natural fats, Sulfated esters, Sulfated alkanolamides, the moisture content below 10% in order to prevent alkylphenol ethoxylates and sulfated alkylphenols; regrowth of microorganisms. A moisture content below a non-ionic Surfactant selected from the group consisting 10% is a usual standard of the industry to prevent a of alcohol ethoxylates, ethoxylated aliphatic alcohols, growth of microorganisms. polyoxyethylene Surfactants, carboxylic esters, poly 0317. It is understood that the examples and embodi ethylene glycol esters, anhydrosorbitol ester and its ments described herein are for illustrative purposes only and ethoxylated derivatives, glycol esters of fatty acids, that various modifications or changes in light thereof will be carboxylic amides, monoalkanolamine condensates Suggested to persons skilled in the art and are to be included and polyoxyethylene fatty acid amides; within the present invention and scope of the appended a cationic Surfactant selected from the group consisting of claims. quaternary ammonium salts, amines with amide link ages, polyoxyethylene alkyl and alicyclic amines, 4-N. 1.-80. (canceled) N,N',N'-tetrakis substituted ethylenediamines and 5.2- 81. A composition of water-soluble ingredients (CWSI) alkyl-1-hydroxyethyl 2-imidazolines: which when solubilized in water (W) and at least one an amphoteric Surfactant selected from the group consist agriculturally acceptable solvent, forms a synergistic com ing of N-coco 3-aminopropionic acid and its Sodium position for the control of pathogens and/or the prevention salt, N-tallow 3-iminodipropionate and its disodium of diseases associated with the presence of said pathogens in salt, N-carboxymethyl N-dimethyl N-9 octadecenyl and/or on seeds, wherein said composition of water-soluble ammonium hydroxide, and N-cocoamidethyl N-hy ingredients (CWSI) is to be dissolved in water in a weight droxyethylglycine and its sodium salt; or ratio (CWSI)/(W) in the range from 1:100 to 1:4: an organic biodegradable and biobased surfactant. wherein (i) said composition of water-soluble ingredients 84. The composition of claim 83, wherein said powdered (CWSI) is: composition of water-soluble ingredients (PCWSI) is a dry, a liquid preformed peracetic acid, or an in situ generated water-soluble mixture of: peracetic acid obtained from a powdered composition i. 58% w/w of the solid hydrogen peroxide precursor: of water-soluble ingredients (PCWSI) defining a per ii. 18% w/w of the pH adjusting agent; US 2016/0295860 A1 Oct. 13, 2016

iii. 20% w/w of the acetylating agent; Sulfated natural fats, Sulfated esters, Sulfated alkano iv. 4% w/w of a wetting agent as defined in claim 3; and lamides, alkylphenol ethoxylates and Sulfated alky wherein (i), (ii) and (iii) represent the peracetic acid lphenols; precursor, and wherein when 2 g of said dry, water a non-ionic Surfactant selected from the group consist soluble mixture of (i), (ii), (iii) and (iv) is admixed with ing of alcohol ethoxylates, ethoxylated aliphatic 1000 g of water, 100 to 250 ppm of peracetic acid alcohols, polyoxyethylene Surfactants, carboxylic (PAA) are generated in situ at pH 9.0+3; esters, polyethylene glycol esters, anhydrosorbitol wherein the acylating agent is acetylsalicylic acid or ester and its ethoxylated derivatives, glycol esters of tetraacetylethylenediamine (TAED); fatty acids, carboxylic amides, monoalkanolamine wherein the Solid hydrogen peroxide precursor is sodium condensates and polyoxyethylene fatty acid amides; perborate, Sodium percarbonate, ammonium percar a cationic Surfactant selected from the group consisting bonate, sodium peroxyhydrate, calcium peroxide, of quaternary ammonium salts, amines with amide Sodium peroxide, Sodium perborate monohydrate, linkages, polyoxyethylene alkyl and alicyclic Sodium perborate tetrahydrate, sodium persulfate, amines, 4-N.N.N',N'-tetrakis substituted ethylenedi potassium monoperSulfate, perphosphate, magnesium amines and 5.2-alkyl-1-hydroxyethyl 2-imidazo peroxide, Zinc peroxide, urea hydrogen peroxide, per lines; hydrate of urea, thiourea dioxide, or a mixture thereof; an amphoteric Surfactant selected from the group con and sisting of N-coco 3-aminopropionic acid and its wherein the pH adjusting agent is Sulfuric acid, citric acid, sodium salt, N-tallow 3-iminodipropionate and its phosphoric acid, nitric acid, hydrochloric acid, glycolic disodium salt, N-carboxymethyl N-dimethyl N-9 acid, formic acid, acetic acid, hydrofluoric acid, nitrous octadecenyl ammonium hydroxide, and N-cocoami acid, hydrocyanic acid, benzoic acid, carboxylic acid, dethyl N-hydroxyethylglycine and its sodium salt; or lactic acid, acetic acid, oxalic acid, Sulfamic acid, an organic biodegradable and biobased Surfactant. phosphorous acid, dipicolinic acid, urea.HCl, boric 88. The synergistic composition of claim 87, wherein said acid, or a mixture thereof. powdered composition of water-soluble ingredients 85. A synergistic composition for the control of pathogens (PCWSI) is a dry, water-soluble mixture of: and/or the prevention of diseases associated with the pres i. 58% w/w of the solid hydrogen peroxide precursor: ence of said pathogens in and/or on seeds wherein said synergistic composition comprises: ii. 18% w/w of the pH adjusting agent; water (W); iii. 20% w/w of the acetylating agent; the composition of water-soluble ingredients (CWSI) iv. 4% w/w of a wetting agent as defined in claim 7; and defined in claim 81; and wherein (i), (ii) and (iii) represent the peracetic acid at least one agriculturally acceptable solvent which is precursor, and wherein when 2 g of said dry, water soluble in water (W) and selected from the group soluble mixture of (i), (ii), (iii) and (iv) is admixed with consisting of an agriculturally acceptable alcohol, gly 1000 g of water, 100 to 250 ppm of peracetic acid col ether, propylene glycol and ethylene glycol, (PAA) are generated in situ at pH 9.0+3; wherein the composition of water-soluble ingredients wherein the acylating agent is acetylsalicylic acid or (CWSI) is contacted with water (W), in a weight ratio tetraacetylethylenediamine (TAED); (CWSI)/(W) ranging from 1:100 to 1:4, the at least one wherein the Solid hydrogen peroxide precursor is: agriculturally acceptable solvent either forming a mix Sodium perborate, sodium percarbonate, ammonium per ture with the water (W) or being added to a solution of carbonate, sodium peroxyhydrate, calcium peroxide, peracetic acid resulting from the contact of the com Sodium peroxide, Sodium perborate monohydrate, position of water-soluble ingredients (CWSI) with Sodium perborate tetrahydrate, Sodium persulfate, water, to form the synergistic composition; and potassium monoperSulfate, perphosphate, magnesium wherein the at least one agriculturally acceptable solvent peroxide, Zinc peroxide, urea hydrogen peroxide, per is present in the synergistic composition in Such an hydrate of urea, thiourea dioxide, or a mixture thereof; amount that it represents from 2% by volume to 70% by and Volume, of the total Volume of the Synergistic compo wherein the pH adjusting agent is Sulfuric acid, citric acid, sition. phosphoric acid, nitric acid, hydrochloric acid, glycolic 86. The synergistic composition of claim 85, wherein said acid, formic acid, acetic acid, hydrofluoric acid, nitrous at least one agriculturally acceptable solvent is an alcohol of acid, hydrocyanic acid, benzoic acid, carboxylic acid, formula ROH where R represents a linear alkyl group lactic acid, acetic acid, oxalic acid, Sulfamic acid, having from 1 to 6 carbon atoms, or a branched alkyl group phosphorous acid, dipicolinic acid, urea.HCl, boric having from 3 to 6 carbon atoms. acid, or a mixture thereof. 87. The synergistic composition of claim 86, wherein said 89. The synergistic composition of claim 88, wherein composition of water-soluble ingredients further comprises: when the at least one agriculturally acceptable solvent is (iii) at least one wetting agent, wherein the at least one selected from the group consisting of C-C alcohols and Wetting agent is: glycol ethers, said aqueous synergistic composition being an anionic Surfactant selected from the group consisting further for the prevention of the release of mucilage from of carboxylates, Sulfonates, petroleum Sulfonates, seeds. alkylbenzenesulfonates, naphthalene Sulfonates, 90. The synergistic composition of claim 89, wherein olefin Sulfonates, alkyl Sulfates, sulfated natural oils, seeds are flax seeds or chia seeds. US 2016/0295860 A1 Oct. 13, 2016 24

91. The synergistic composition of claim 89, wherein said oncillo, maya nut, mongongo, oak acorns, ogbono nut, synergistic composition is for an application to the seeds by paradise nut, pili nut, walnut, black walnut, and water spraying, Vaporizing, soaking, fumigating, or electrostatic caltrop; spraying. nut-like gymnosperm seeds selected from the group con 92. The synergistic composition of claim 91, wherein the sisting of cycads, ginkgo, Gnetum gnemon, juniper, pathogens are selected from the group consisting of viruses, monkey-puzzle, pine nuts, and podocarps; bacteria, fungi, yeasts and moulds. seeds selected from the group consisting of cempedak, 93. The synergistic composition of claim 92, for the coffee, egusi, euryale ferox, fluted pumpkin, hemp further prevention of mucilage from seeds. seed, jackfruit, lotus seed, Malabar gourd, pumpkin seed, Sunflower seed, sesame seed, and Tahini; 94. The synergistic composition of claim 91 wherein the beans selected from the group consisting of bambara pathogens are: groundnut, chickpeas, cowpeas, black eyed pea, dry Selected from the group consisting of E. Coli, Listeria beans, fava, broad beans, hyacinth bean, lablab, lentils, monocytogenes and Salmonella spp.; or lupins, Moringa oleifera, peas, peanuts, pigeon peas, Selected from the group consisting of Agrobacterium spp., Sterculia, Velvet beans, winged beans, yam beans, and Burkholderia spp., Clavibacter spp., Corynebacterium Soybeans; spp., Erwinia spp., Pseudomonas spp., Ralstonia spp., seeds for sprouting selected from the group consisting of Rhizomonas spp., Xanthomonas spp., and Xylella spp.; alfalfa, clover, fenugreek, lentil, pea, chickpea, mung O bean, soybean; oat, wheat, maize, rice, barley, rye, Selected from the group consisting of Albugo spp., Alter kamut, quinoa, amaranth, buckwheat; sesame, Sun naria spp., Armillaria spp., Aspergillus spp., Athelia flower, almond, hazelnut, linseed, peanut; brassicas, spp., Bipolaris spp., Botryosphaeria spp., Botryotinia crucifers, broccoli, cabbage, watercress, mustard, spp., Botrytis spp., Bremia spp., Capnodium spp., miZuna, radish, daikon, rocket, tatsoi and turnip; carrot, Ceratobasidium spp., Ceratocystis spp., Cercospora celery, fennel, parsley, onion, leek, green onion, me spp., Choanephora spp., Claviceps spp., Corynespora negi, spinach, lettuce, milk thistle, and lemon grass; spp., Cronartium spp., Cryphonectria spp., Cylindro seed spices selected from the group consisting of awain, cladium spp., Cytospora spp., Diaporthe spp., Diplodia carom seeds, alligator pepper, mbongo Spice, mbongo spp., Dreschlera spp., Elsinoe spp., Erexohilum spp., chobi, hepper pepper, allspice, anise, aniseed myrtle, Erysiphe spp., Eutpha spp., Exobasidium spp., annatto, borage, black cardamom, black mustard, blue Fusarium spp., Gaeumannomyces spp., Gliocladium fenugreek, blue melilot, brown mustard, caraway, car spp., Gymnosporangium spp., Heterobasidium spp., damom, celery seed, clove, coriander seed, cumin, dill Hypoxylon spp., Kutilakesa spp., Lophiodermium spp., seed, fennel, fenugreek, grains of paradise, grains of Magnaporthe spp., Melampsora spp., Monilinia spp., Selim, Kani pepper, juniper berry, kala Zeera, kalajira, Mycosphaerella spp., Myrothecia spp., Nectriella spp., black cumin, kawakawa seeds, keluak, kluwak, kepay Nematospora spp., Oidium spp., Olpidium spp., Oph ang, kokam seed, korarima, Ethiopian cardamom, false iostoma spp., Penicillium spp., Peronospora spp., Pha cardamom, mace, mahlab, Saint Lucie cherry, black kOspora spp., Phoma spp., Phomopsis spp., Phrag mustard seed, brown mustard seed, white mustard seed, midium spp., Phyllactinia spp., Physoderma spp., yellow mustard seed, nigella, kalonji, black caraway, Phytophthora spp., Plasmodiophora spp., Plasmopara black onion seed, njangsa, djansang, nutmeg, black spp., Pseudoperonospora spp., Puccinia spp., Pythium pepper seed, green pepper seed, black pepper seed, spp., Rhizoctonia spp., Rhizopus spp., Rhytisma spp., white pepper seed, star anise, Sumac, Szechuan pepper, Sclerotinia spp., Sclerotium spp., Spongospora spp., Sichuan pepper, Vanilla, and wattleseed; Synchytrium spp., Taphrina spp., Thanatephorus spp., seeds of crops transplantable from greenhouse to field and Thielaviopsis spp., Tilletia spp., Uncinula spp., Uro Selected from the group consisting of basil, bell pepper, cystis spp., Ustilago spp., Valso spp., Venturia spp., broccoflower, broccoli, brussels sprouts, cabbage, can Verticillium spp., and Xwlaria spp. taloupe, cauliflower, celery, cucumber, eggplant, head 95. The synergistic composition of claim 94, wherein the lettuce, honeydew, muskmelon, onion, radicchio, seeds are edible seeds, grains raw and/or cooked, seeds of romaine lettuce, squash, tobacco, tomato, and water transplantable crops, or seeds for sprouting, and wherein the melon; or seeds are: seeds of medical marijuana. 96. The synergistic composition of claim 95, wherein the cereals selected from the group consisting of barley, seeds are selected from the group consisting hemp, flax and fonio, maize, pearl millet, oats, palmer's grass, rice, chia seeds. rye, Sorghum, spelt, teff, triticale, wheat, and wild rice; 97. A method for the control of pathogens and/or the pseudocereals selected from the group consisting of prevention of diseases associated with the presence of said breadnut, buckwheat, cattail, chia, flax, grain amaranth, pathogens in and/or on seeds, said method comprising a step kafiiwa, pitseed goosefoot, quinoa, and wattleseed; of contacting said seeds with a synergistic composition as nuts selected from the group consisting of almonds, defined in claim 85, wherein the synergistic composition is coconuts, peanuts and cashews, beech, brazil nut, contacted with seeds in Such an amount that said at least one candlenut, cashew, chestnuts, Chinese chestnut, Sweet agriculturally acceptable solvent represents less than 10% by chestnut, coconut, colocynth, Cucurbita ficifolia, fil Volume, as final concentration of the at least one agricul bert, Gevulina avellana, hickory, pecan, shagbark turally acceptable solvent admixed with seeds. hickory, Terminalia catappa, hazelnut, Indian beech, 98. The method of claim 97, wherein in the synergistic kola nut, macadamia, Malabar chestnut, pistacia, mam composition said at least one agriculturally acceptable sol US 2016/0295860 A1 Oct. 13, 2016

vent is an alcohol of formula ROH where R represents a 101. The method of claim 97, for the further prevention of linear alkyl group having from 1 to 6 carbon atoms, or a mucilage from seeds. branched alkyl group having from 3 to 6 carbon atoms. 102. The method of claim 97, wherein the pathogens are 99. The method of claim 97, wherein the synergistic selected from the group consisting of E. Coli, Listeria composition of water-soluble ingredients further comprises: monocytogenes and Salmonella spp.; or (iii) at least one wetting agent, wherein the at least one selected from the group consisting of Agrobacterium spp., Wetting agent is: Burkholderia spp., Clavibacter spp., Corynebacterium an anionic Surfactant selected from the group consisting spp., Erwinia spp., Pseudomonas spp., Ralstonia spp., of carboxylates, Sulfonates, petroleum Sulfonates, Rhizomonas spp., Xanthomonas spp., and Xylella spp.; alkylbenzenesulfonates, naphthalene Sulfonates, O olefin Sulfonates, alkyl Sulfates, sulfated natural oils, selected from the group consisting of Albugo spp., Alter Sulfated natural fats, Sulfated esters, Sulfated alkano naria spp., Armillaria spp., Aspergillus spp., Athelia lamides, alkylphenol ethoxylates and Sulfated alky spp., Bipolaris spp., Botryosphaeria spp., Botryotinia lphenols; spp., Botrytis spp., Bremia spp., Capnodium spp., a non-ionic Surfactant selected from the group consist Ceratobasidium spp., Ceratocystis spp., Cercospora ing of alcohol ethoxylates, ethoxylated aliphatic spp., Choanephora spp., Claviceps spp., Corynespora alcohols, polyoxyethylene Surfactants, carboxylic spp., Cronartium spp., Cryphonectria spp., Cylindro esters, polyethylene glycol esters, anhydrosorbitol cladium spp., Cytospora spp., Diaporthe spp., Diplodia ester and its ethoxylated derivatives, glycol esters of spp., Dreschlera spp., Elsinoe spp., Erexohilum spp., fatty acids, carboxylic amides, monoalkanolamine Erysiphe spp., Eutypha spp., Exobasidium spp., condensates and polyoxyethylene fatty acid amides; Fusarium spp., Gaeumannomyces spp., Gliocladium a cationic Surfactant selected from the group consisting spp., Gymnosporangium spp., Heterobasidium spp., of quaternary ammonium salts, amines with amide Hypoxylon spp., Kutilakesa spp., Lophiodermium spp., linkages, polyoxyethylene alkyl and alicyclic Magnaporthe spp., Melompsora spp., Monilinia spp., amines, 4-N.N.N',N'-tetrakis substituted ethylenedi Mycosphaerella spp., Myrothecia spp., Nectriella spp., amines and 5.2-alkyl-1-hydroxyethyl 2-imidazo Nematospora spp., Oidium spp., Olpidium spp., Oph lines; iostoma spp., Penicillium spp., Peronospora spp., Pha an amphoteric Surfactant selected from the group con kospora spp., Phoma spp., Phomopsis spp., Phrag sisting of N-coco 3-aminopropionic acid and its midium spp., Phyllactinia spp., Physoderma spp., sodium salt, N-tallow 3-iminodipropionate and its Phytophthora spp., Plasmodiophora spp., Pasmopara disodium salt, N-carboxymethyl N-dimethyl N-9 spp., Pseudoperonospora spp., Puccinia spp., Pythium octadecenyl ammonium hydroxide, and N-cocoami spp., Rhizoctonia spp., Rhizopus spp., Rhytisma spp., dethyl N-hydroxyethylglycine and its sodium salt; or Sclerotinia spp., Sclerotium spp., Spongospora spp., an organic biodegradable and biobased surfactant. Synchytrium spp., Taphrina spp., Thanatephorus spp., 100. The method of claim 99, wherein the powdered Thielaviopsis spp., Tilletio spp., Uncinula spp., Uro composition of water-soluble ingredients (PCWSI) is a dry, cystis spp., Ustilago spp., Valso spp., Venturia spp., water-soluble mixture of: Verticillium spp., and Xylaria spp. i. 58% w/w of the solid hydrogen peroxide precursor: 103. The method of claim 102, wherein the seeds are ii. 18% w/w of the pH adjusting agent; edible seeds, grains raw and/or cooked, seeds of transplant iii. 20% w/w of the acetylating agent; able crops, or seeds for sprouting; and wherein the seeds are: iv. 4% w/w of a wetting agent as defined in claim 7; and cereals selected from the group consisting of barley, wherein (i), (ii) and (iii) represent the peracetic acid fonio, maize, pearl millet, oats, palmer's grass, rice, precursor, and wherein when 2 g of said dry, water rye, Sorghum, spelt, teff, triticale, wheat, and wild rice; soluble mixture of (i), (ii), (iii) and (iv) is admixed with 1000 g of water, 100 to 250 ppm of peracetic acid pseudocereals selected from the group consisting of (PAA) are generated in situ at pH 9.0+3; breadnut, buckwheat, cattail, chia, flax, grain amaranth, wherein the acylating agent is acetylsalicylic acid or kafiiwa, pitseed goosefoot, quinoa, and wattleseed; tetraacetylethylenediamine (TAED); nuts selected from the group consisting of almonds, wherein the Solid hydrogen peroxide precursor is sodium coconuts, peanuts and cashews, beech, brazil nut, perborate, Sodium percarbonate, ammonium percar candlenut, cashew, chestnuts, Chinese chestnut, Sweet bonate, sodium peroxyhydrate, calcium peroxide, chestnut, coconut, colocynth, Cucurbita ficifolia, fil Sodium peroxide, Sodium perborate monohydrate, bert, Gevulina avellana, hickory, pecan, shagbark Sodium perborate tetrahydrate, sodium persulfate, hickory, Terminalia catappa, hazelnut, Indian beech, potassium monoperSulfate, perphosphate, magnesium kola nut, macadamia, Malabar chestnut, pistacia, mam peroxide, Zinc peroxide, urea hydrogen peroxide, per oncillo, maya nut, mongongo, oak acorns, ogbono nut, hydrate of urea, thiourea dioxide, or a mixture thereof; paradise nut, pili nut, walnut, black walnut, and water and caltrop; wherein the pH adjusting agent is Sulfuric acid, citric acid, nut-like gymnosperm seeds selected from the group con phosphoric acid, nitric acid, hydrochloric acid, glycolic sisting of cycads, ginkgo, Gnetum gnemon, juniper, acid, formic acid, acetic acid, hydrofluoric acid, nitrous monkey-puzzle, pine nuts, and podocarps; acid, hydrocyanic acid, benzoic acid, carboxylic acid, seeds selected from the group consisting of cempedak, lactic acid, acetic acid, oxalic acid, Sulfamic acid, coffee, egusi, euryale ferox, fluted pumpkin, hemp phosphorous acid, dipicolinic acid, urea.HCl, boric seed, jackfruit, lotus seed, Malabar gourd, pumpkin acid, or a mixture thereof. seed, Sunflower seed, sesame seed, and Tahini; US 2016/0295860 A1 Oct. 13, 2016 26

beans selected from the group consisting of bambara black onion seed, njangsa, djansang, nutmeg, black groundnut, chickpeas, cowpeas, black eyed pea, dry pepper seed, green pepper seed, black pepper seed, beans, fava, broad beans, hyacinth bean, lablab, lentils, white pepper seed, star anise, Sumac, Szechuan pepper, lupins, Moringa oleifera, peas, peanuts, pigeon peas, Sichuan pepper, Vanilla, and wattleseed; Sterculia, Velvet beans, winged beans, yam beans, and seeds of crops transplantable from greenhouse to field and Soybeans; Selected from the group consisting of basil, bell pepper, seeds for Sprouting selected from the group consisting of broccoflower, broccoli, brussels sprouts, cabbage, can alfalfa, clover, fenugreek, lentil, pea, chickpea, mung taloupe, cauliflower, celery, cucumber, eggplant, head bean, soybean; oat, wheat, maize, rice, barley, rye, lettuce, honeydew, muskmelon, onion, radicchio, kamut, quinoa, amaranth, buckwheat; sesame, Sun romaine lettuce, squash, tobacco, tomato, and water flower, almond, hazelnut, linseed, peanut, brassicas, melon; or crucifers, broccoli, cabbage, watercress, mustard, seeds of medical marijuana. miZuna, radish, daikon, rocket, tatsoi and turnip; carrot, 104. The method of claim 103, wherein said synergistic celery, fennel, parsley, onion, leek, green onion, me composition is for an application to the seeds by spraying, negi, spinach, lettuce, milk thistle, and lemon grass; Vaporizing, Soaking, fumigating, or electrostatic spraying. seed spices selected from the group consisting of awain, 105. The method of claim 104, wherein said synergistic carom seeds, alligator pepper, mbongo Spice, mbongo composition is for an application to the seeds by spraying chobi, hepper pepper, allspice, anise, aniseed myrtle, and keeping the Synergistic composition in contact with the annatto, borage, black cardamom, black mustard, blue seeds and grains for 2 minutes to 48 hours before drying. fenugreek, blue melilot, brown mustard, caraway, car 106. The method of claim 105, wherein the synergistic damom, celery seed, clove, coriander seed, cumin, dill composition is kept in contact with the seeds and grains for seed, fennel, fenugreek, grains of paradise, grains of 24 hours before drying. Selim, Kani pepper, juniper berry, kala Zeera, kalajira, 107. The method of claim 105, wherein seeds are dried black cumin, kawakawa seeds, keluak, kluwak, kepay after contact with the Synergistic composition and contact ang, kokam seed, korarima, Ethiopian cardamom, false time, to reduce the moisture content of the seeds below 10% cardamom, mace, mahlab, Saint Lucie cherry, black and further contribute to prevent a regrowth of microorgan mustard seed, brown mustard seed, white mustard seed, 1SS. yellow mustard seed, nigella, kalonji, black caraway,