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(12) Patent Application Publication (10) Pub. No.: US 2016/0295860 A1 DAGHER Et Al US 2016O295860A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2016/0295860 A1 DAGHER et al. (43) Pub. Date: Oct. 13, 2016 (54) A NOVEL COMPOSITION AND METHOD OF AOIN 25/00 (2006.01) USE TO CONTROL PATHOGENS AND AOIN 31/02 (2006.01) PREVENT DISEASES IN SEEDS (52) U.S. Cl. CPC ............... A0IN 37/16 (2013.01); A0IN 25/00 (71) Applicant: AGRI-NEO INC., Toronto (CA) (2013.01): A0IN 31/02 (2013.01); A23B 9/26 (2013.01); A23B 9/30 (2013.01); A23V (72) Inventors: Fadi DAGHER, Laval (CA); Nicholas 2002/00 (2013.01) DILLON, Toronto (CA); Kenneth Sherman UNGAR, Scarborough (CA) (57) ABSTRACT (73) Assignee: AGRI-NEO INC., Toronto, ON (CA) The invention relates to a composition of water-soluble ingredients (CWSI) which when solubilized in water (W) (21) Appl. No.: 15/038,211 and either in the presence of a wetting agent and/or in the presence of at least one agriculturally acceptable solvent, (22) PCT Fed: Nov. 13, 2014 forms a synergistic composition useful for the control of (86) PCT No.: PCT/CA2O14/051088 pathogens and/or the prevention of diseases associated with the presence of said pathogens in and/or on seeds. Said S 371 (c)(1), composition of water-soluble ingredients (CWSI) comprises (2) Date: May 20, 2016 at least one oxidizer in liquid form or solid form, or a precursor thereof in liquid or solid form, said at least one Related U.S. Application Data agriculturally acceptable solvent is soluble in water (W); the (60) Provisional application No. 61/907,560, filed on Nov. water-soluble ingredients (CWSI) is dissolved in the water 22, 2013. (W), in a weight ratio (CWSI)/(W) ranging from 1:100 to 1:4, and when present, said at least one agriculturally Publication Classification acceptable solvent represents from 2% by volume to 70% by Volume of the total Volume of the Synergistic composition to (51) Int. C. be formed. The invention also relates to a synergistic com AOIN 37/16 (2006.01) position for the control of pathogens and/or the prevention A23B 9/30 (2006.01) of diseases associated with the presence of said pathogens in A23B 9/26 (2006.01) and/or on seeds, and a method and a use of the same. Patent Application Publication Oct. 13, 2016 Sheet 1 of 3 US 2016/029586.0 A1 3,500,000 cc S 3,000,000 joio 2,500,000 rior iC 2,000,000 circ mock 1,500,000 Neo-Pire 1,000,000 Neo-Pre EEO 500,000 in +21. hrs +48 hrs --72 rS 'YYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYY Figure S s----------------------------------------------------------------------------------------------------------------------------------------------- k s 1,200,000 k k k k k k 1,000,000 800,000 600,000 400,000 200,000 Mock (H2O) Neo-Pure YYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYY Figure 2 Patent Application Publication Oct. 13, 2016 Sheet 2 of 3 US 2016/029586.0 A1 1,000,000 800,000 600,000 d 400,000 d 200,000 () --21 hrs s: Mock (H2O) Neo-Pie EtOH \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\rs 20,000,000 18,000,000 15,000,000 14,000,000 12,000,000 ifu Now 10,000,000 AAA N i 8,000,000 --------- X Ea. S. Neo-Pony 6,000,000 Sas S. Neo-P - EtOH 4,000,000 t -------------. Sri& 2,000,000 cc Sar NoScN o St. NY, KNX NX: NZ rs starting + 3 hr of in +24 his +48 hrs goint \\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\\ Figure 4 Patent Application Publication Oct. 13, 2016 Sheet 3 of 3 US 2016/029586.0 A1 20,000,000 18,000,000 16,000,000 14,000,000 12,000,000 10,000,000 N. 83tooger 3,000,000 is S 5,000,000 iss S. Š 4,000,000 iss 2,000,000 or C - Figure 5 20,000,000 cc 18,000,000 16,000,000 14,000,000 42,700,000 cu. s 12,000,000 cc 10,000,000 in 8,000,000 in 6,000,000 in 4,000,000 - 24ttoori,66;for 2,000,000 as 1430,000 O r r r JC Neo-Pure EtO Figure 6 US 2016/0295860 A1 Oct. 13, 2016 A NOVEL COMPOSITION AND METHOD OF ent application No. 2,569,025; International PCT patent USE TO CONTROL PATHOGENS AND application WO95/02330 and EP patent application No. 0 PREVENT DISEASES IN SEEDS 648 418). 0005 Also, peracetic acid (also known under the trade FIELD OF THE INVENTION name peracid) is a strong oxidizing agent which is known for having virucidal, bactericidal, fungicidal and algaecidal 0001. The invention relates to the field of agriculture, properties. Peracetic acid was patented in 1950 for the food safety and post-harvest food treatments to address the treatment of raw plant tissue, especially for the treatment of lack of useful methods to prevent bacterial contamination of fruits and vegetables, to reduce spoilage from bacteria and dry raw agricultural commodities. The novel composition fungi destined for processing (U.S. Pat. No. 2,512.640). provides unexpectedly high degree of control required for Nowadays, peracetic acid is commonly use in food process food safety. The invention also relates to a method of use ing and handling as a sanitizer for food contact surfaces and thereof. as a disinfectant for fruits, vegetables, meat and eggs (NOSB TAP Materials database compiled by OMRI, Nov. 3, 2000, 0002 More particularly, the invention relates to a com 7 pages). In the production of fruits and vegetables, per position of water-soluble ingredients, said water-soluble acetic aqueous Solutions have been Suggested to control ingredients comprising at least one oxidizer, at least one pathogenic organisms on growing plants (U.S. Pat. No. lower alcohol and optionally at least one wetting agent, 6,024,986; U.S. Pat. No. 6,165,483; and U.S. Pat. No. which when solubilized in a water-containing solvent forms 6,238,685). an aqueous, Synergistic composition useful for the control of 0006. As per Applicant’s published international patent pathogens and/or the prevention of diseases associated with application WO 2012/051699, a solution of peracetic acid the presence of said pathogens in and/or on seeds, more generated in situ in combination with a plant defence preferably edible seeds such as hemp seeds, flax seeds or enhancer demonstrated excellent anti-bactericidal and anti chia seeds. Also, the invention relates to an aqueous, Syn fungal properties. More particularly, in this international ergistic composition obtained by dissolving the above-men patent application it was evidenced a synergy of peracetic tioned composition in water, and relates to method and use acid and at least one plant defence enhancer for the control of said aqueous, Synergistic composition for the control of of pathogens in and onto growing plants. Furthermore, pathogens and/or the prevention of diseases associated with according to a particularly preferred embodiment of said the presence of said pathogens in and/or on seeds, more published international patent application WO2012/051699, preferably edible seeds such as hemp seeds, flax seeds or the presence of a Surfactant in a synergistic mix of a chia seeds. precursor of peracetic acid and potassium silicate (i.e. a SAR inducer), enhances the release of peracetic acid when BACKGROUND OF THE INVENTION admixed with water. 0007. However, concerning seeds, more particularly 0003 Published international application No. WO2007/ seeds having external shells, especially edible seeds Such as O92180 describes a fertilizer composition for application to hemp seeds, flax seeds and chia seeds, the person skilled in a seed, plant, growth medium or growth solution, said the art notes that existing compositions do not allow an composition comprising an oxidizing agent wherein bio oxidizer to cover the surface of the seeds efficiently in order available oxygen is released upon contact to the composition to kill pathogens present thereon and/or therein. with water, optionally a solvent Such as methyl ketone, 0008. Therefore, there was still a strong need for a methyl isobutyl ketone, cyclohexanonon, Xylenes, toluene, composition allowing to obtain an efficient control and/or chlorobenzene, paraffins, kerosene, white oil, alcohols, treatment of diseases associated with seeds, especially con methylnaphthalene, trimethylbenzene, trichloroethylene, cerning seeds having external shells, especially edible grains N-methyl-2-pyrrolidone and tetrahydrofurfuryl alcohol and seeds Such as hemp seeds. Also, there was a strong need (THFA), and eventually other additives selected from the for method and use allowing to control and/or treat effi group consisting of companion cations, cation reducing ciently seeds having pathogens present thereon. agents, pH modulators, nutrients, organic compounds, pen 0009. Also, it is to be noted that literature did not etrants, microorganisms, pesticides, fungicides, insecticides, recommend using alcohol on seeds because the person nematocides, herbicides, water trapping agents, enzymes, skilled in the art knows that alcohol at low dosage does not Surfactants, wetting agents, spreaders, stickers and growth kill bacteria. In this regard, reference can be made to the hormones. This published international application content of the article of Mena et al., “Influence of Ethanol on WO2007/092180 further relates to a method of use of said Probiotic and Culture Bacteria Lactobacillus bulgaricus and fertilizer composition. However, this published international Streptococcus thermophilus within a Therapeutic Product’. application does not provide a novel Solution to control the Open Journal of Medical Microbiology, 2012, 2, 70-76. In high level of pathogens on edible food commodities. this article Mena et al. showed that ethanol added to yogurt 0004 Peracetic acid (CHO) in an aqueous solution is does not affect the count of Lactobacillus sp. a mixture which is further comprising acetic acid 0010 Also, it is to be noted in addition to the teaching of (CHCOOH) and hydrogen peroxide (HO).
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