DOCSLIB.ORG

Brucellosis 2014 International Research Conference

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Brucellosis 2014 International Research Conference SCHEDULE 2014 Federal Institute for Risk Assessment Brucellosis 2014 International Research Conference BERLIN | 9–12 September 2014 Imprint Abstracts Brucellosis 2014 International Research Conference All authors are responsible for the content of their respective abstracts. Federal Institute for Risk Assessment Communication and Public Relations Office Max-Dohrn-Straße 8–10 10589 Berlin Germany Berlin 2014 222 Pages Photo: flobox/Quelle: PHOTOCASE Printing: cover, content pages and bookbinding BfR-printing house Brucellosis 2014 International Research Conference 3 Welcoming Addresses Andreas Hensel President of the Federal Institute for Risk Assessment (BfR) Welcoming address by the President of the Federal Institute for Risk Assessment More than 100 years after the first description of Micrococcus melitensis by Sir David Bruce, brucellosis is still of major public health concern both in endemic and non-endemic countries all over the world. The impact on animal and human health is tremendous and eradication and control of this zoonotic disease remains a global and interdisciplinary challenge. Alt- hough eradicated in German livestock brucellosis is still a matter of interest because it has emerged as a disease among immigrants associated with diagnostic delays, possibly result- ing in treatment failures, relapses, chronic courses, focal complications, and a high case- fatality rate. The identification of health risks is the guiding principle for our work in the field of food safety and consumer protection. A total of 800 employees spare no effort to prove that no risk is more fun . I am delighted to host the Brucellosis 2014 International Research Conference at the Federal Institute for Risk Assessment, here in Berlin, the capital city of Germany, which was home and domain of so many Nobel laureates. Emil von Behring got the first Nobel Prize in Medi- cine for his work on serum therapy, especially its application against diphtheria; Robert Koch first identified pathogenic microorganisms as the cause of infectious diseases such as tuber- culosis, anthrax and cholera; and Paul Ehrlich developed the first antibiotic drug to treat syphilis and is regarded as the founder of chemotherapy. By the way, Robert Koch is proba- bly the most famous staff member of the Imperial Health Agency which is the predecessor of our institution, the Federal Institute for Risk Assessment. Berlin is a breakthrough city combining tradition and modernity and you have to pass the Brandenburger Tor to enjoy its spirit of freedom and creativity. From 9 to 12 September 2014 my team and I will do everything for you to ensure that you can feel like ‘Ich bin ein Berliner’. It is a great honor for us to welcome the Brucella research community and I wish you an in- spiring meeting with fruitful discussions. 4 Abstracts Karin Schwabenbauer Head of Directorate Animal Health, Animal Welfare of the Federal Ministry of Food and Agriculture (BMEL) President of the World Organisation for Animal Health (OIE) Welcoming address by the German Federal Ministry of Food and Agriculture The control of zoonotic diseases has been a focus of the Federal Ministry's scientific support for many years. For some time we have known that a one-sided view of animal populations, public health, food safety and the security of global trade cannot provide the large-scale suc- cess in the fight against zoonotic diseases which we wish to achieve, and which citizens ex- pect us to achieve. It is only if health authorities, veterinary authorities, the agricultural sector, the food industry, the scientific community and an informed public work together that we will succeed, in the sense of ‘One Health’, in attaining our goal of successfully controlling zoono- tic diseases. In Germany we therefore launched the ‘National Zoonotic Research’ platform in 2006 which incorporates all necessary scientific disciplines. One distinctive feature of this programme is that it only supports projects that are multidisciplinary. Germany is in the hap- py position of its cattle, sheep and goat stocks having been officially free of brucellosis since 2000 - only a few sporadic outbreaks have been reported since. This success has been the result of hard work. It is only possible to maintain this status through an ongoing, legally bind- ing national control procedure, substantial funds and a nationwide monitoring system that is still in place. This successful example of disease control can act as encouragement for, and a basis for control procedures in, those countries in which brucellosis still causes considera- ble difficulties for animals and people. However, account must be taken of the specific socio- economic and socio-cultural situation in each country. Germany, for instance, cannot be compared with countries in which the pastoralism of sheep and goats represents an im- portant means of livelihood. Or in which the health services do not have sufficient funds at their disposal. It is our duty as the international community to provide help and assistance in , one of the most prevalent food-related zoonotic diseases in the world, with the consumption of raw milk and raw-milk cheese representing the most common means of transmission. Alt- hough pasteurisation of milk is consequently a very effective measure for minimising cases of brucellosis in mankind, the best way of eradicating brucellosis in animals and mankind is to monitor animals stocks.Although Germany is deemed officially free of brucellosis, it is still particularly important, in developing more efficient methods of detection, and in particular in assessing individual animals, to achieve success at international level and thus contribute to containing these zoonotic diseases. As false positive diagnoses still lead to considerable losses in international trade, I hope - also in my capacity as President of the OIE - that this conference will provide corresponding proposals that can then be incorporated into the OIE Manual. I wish you all the best for this conference; I hope that you will have stimulating discussions that result in new ideas for successfully fighting zoonotic diseases. Brucellosis 2014 International Research Conference 5 Thomas Mettenleiter President of the Friedrich-Loeffler-Institut (FLI) Welcoming address by the President of the Friedrich-Loeffler-Institut Brucellosis accompanied mankind as a life threatening zoonosis and burden for livestock since the domestication of sheep, goats, cattle and pigs to improve quality of human life. Proof of high disease prevalence in humans can already be found in the skeletal remains of individuals from ancient Egypt or Roman Pompeii. The importance of brucellosis for modern human and veterinary public health is demonstrated by approx. 500 000 new human infec- tions worldwide every year, its rapid re-emergence in developing countries and countries struck by civil disruption, and its continuing presence around the Mediterranean basin and the Levant despite enormous efforts for control presently supported by the EU. Therefore, research on brucellosis epidemiology, prophylaxis and control is well within the mission of the Friedrich-Loeffler-Institut as Federal Research Institute for Animal Health and OIE Col- laborating Centre for Zoonoses in Europe. The OIE reference laboratory for brucellosis at FLI currently is engaged in projects in Africa, Southern America, Europe and Asia. With our new research center on the island of Riems, best equipped and modern P3 laboratories and ex- perimental animal facilities are available for cutting-edge research projects. The work of our brucellosis laboratory will also benefit from new facilities to be built at the FLI site in Jena. Personally, I am very glad that FLI can and will continue to make important contributions to worldwide control and eradication of brucellosis inspired by the idea of ‘One world – one health’. Eradication of brucellosis is feasible as demonstrated by the experiences of countries like Germany where brucellosis was eradicated successfully from domestic livestock during the last century by applying consequent veterinary measures. I am also very glad that we have the pleasure to host the Brucellosis 2014 International Research Conference in Ger- many for the first time. I wish you an inspiring meeting, a fruitful exchange of ideas and pleasant days in Berlin, the lively capital of Germany. Brucellosis 2014 International Research Conference 7 Sascha Al Da hou k, Chair Heinrich Neubauer , Co -Chair Federal Institute for Risk Assessment Friedrich -Loeffler -Ins titut Herzlich Willkommen Soo dhawaada دیآد وش Қош келді ңіздер 에 오신 것을 환영합니다 Welkom Bine ai venit वागत छ! Добро пожаловать Sànnu dà zuwà Xush kelibsiz Ласкаво просимо მოგესალმებით Тавтай морилно уу እንክዋን ደህና መጡ ยินดีต้อนรับคุณ Welcome Benvenuto Välkommen Xoş g əldiniz 迎 Bienvenido בברכה 欢 Karibu Καλώς ήλθατε Bem -vindo Mirë se erdhët Vitajte Velkommen Bienvenue ْأھً َو َ ْ ً Tervetuloa आपका वागत है Witamy Üdvözöljük բարի գալուստ Velkommen til Brucellosis 2014 International Research Conference 9 Table of Contents Final Schedule 11 Abstracts 17 Opening Lectures 17 Session 1: Taxonomy, Evolution, Emerging Species 19 Keynote Lecture: Erko Stackebrandt 19 Oral Presentations (O1) 21 Poster Presentations (P1-P7) 22 Session 2: Genomics, Proteomics and Metabolomics 29 Keynote Lecture: Stephan Köhler 29 Oral Presentations (O2-O6) 34 Poster Presentations (P8-P20) 39 Session 3: Host-Pathogen Interaction 53 Keynote Lecture: Sean Crosson 53 Oral Presentations (O7-O10) 55 Poster Presentations (P21-P37)
Load more

Recommended publications
  • Environment-Dependent Variation in Gut Microbiota of an Oviparous Lizard (Calotes Versicolor)
    animals Article Environment-Dependent Variation in Gut Microbiota of an Oviparous Lizard (Calotes versicolor) Lin Zhang 1,2,*,† , Fang Yang 3,†, Ning Li 4 and Buddhi Dayananda 5 1 School of Basic Medical Sciences, Hubei University of Chinese Medicine, Wuhan 430065, China 2 State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237, China 3 School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China; [email protected] 4 College of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, China; [email protected] 5 School of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD 4072, Australia; [email protected] * Correspondence: [email protected] † These authors contributed equally to this work. Simple Summary: The different gut sections potentially provide different habitats for gut microbiota. We found that Bacteroidetes, Firmicutes, and Proteobacteria were the three primary phyla in gut micro- biota of C. versicolor. The relative abundance of dominant phyla Bacteroidetes and Firmicutes exhibited an increasing trend from the small intestine to the large intestine, and there was a higher abun- dance of genus Bacteroides (Class: Bacteroidia), Coprobacillus and Eubacterium (Class: Erysipelotrichia), Parabacteroides (Family: Porphyromonadaceae) and Ruminococcus (Family: Lachnospiraceae), and Family Odoribacteraceae and Rikenellaceae in the hindgut, and some metabolic pathways were higher in the hindgut. Our results reveal the variations of gut microbiota composition and metabolic pathways in Citation: Zhang, L.; Yang, F.; Li, N.; different parts of the lizards’ intestine. Dayananda, B. Environment- Dependent Variation in Gut Abstract: Vertebrates maintain complex symbiotic relationships with microbiota living within their Microbiota of an Oviparous Lizard gastrointestinal tracts which reflects the ecological and evolutionary relationship between hosts and (Calotes versicolor).
    [Show full text]
  • Characterization of Environmental and Cultivable Antibiotic- Resistant Microbial Communities Associated with Wastewater Treatment
    antibiotics Article Characterization of Environmental and Cultivable Antibiotic- Resistant Microbial Communities Associated with Wastewater Treatment Alicia Sorgen 1, James Johnson 2, Kevin Lambirth 2, Sandra M. Clinton 3 , Molly Redmond 1 , Anthony Fodor 2 and Cynthia Gibas 2,* 1 Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] (A.S.); [email protected] (M.R.) 2 Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] (J.J.); [email protected] (K.L.); [email protected] (A.F.) 3 Department of Geography & Earth Sciences, University of North Carolina at Charlotte, Charlotte, NC 28223, USA; [email protected] * Correspondence: [email protected]; Tel.: +1-704-687-8378 Abstract: Bacterial resistance to antibiotics is a growing global concern, threatening human and environmental health, particularly among urban populations. Wastewater treatment plants (WWTPs) are thought to be “hotspots” for antibiotic resistance dissemination. The conditions of WWTPs, in conjunction with the persistence of commonly used antibiotics, may favor the selection and transfer of resistance genes among bacterial populations. WWTPs provide an important ecological niche to examine the spread of antibiotic resistance. We used heterotrophic plate count methods to identify Citation: Sorgen, A.; Johnson, J.; phenotypically resistant cultivable portions of these bacterial communities and characterized the Lambirth, K.; Clinton,
    [Show full text]
  • Product Information Sheet for NR-50276
    Product Information Sheet for NR-50276 Brucella melitensis, Strain 16MΔvjbR abortion and infertility. Transmission from animal to human via contact with infected animal products or through the air may lead to Malta (or undulant) fever, a long debilitating Catalog No. NR-50276 disease treatable by a prolonged course of antibiotics. This reagent is the property of the U.S. Government. Brucella species are recognized as potential agricultural, civilian, and military bioterrorism agents.4 For research use only. Not for human use. Material Provided: Contributor: Each vial contains approximately 0.7 mL of bacterial culture Thomas A. Ficht, Professor, College of Veterinary Medicine in Tryptic Soy broth supplemented with 10% glycerol. and Biological Sciences, Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas, Note: If homogeneity is required for your intended use, please USA purify prior to initiating work. Manufacturer: Packaging/Storage: BEI Resources NR-50276 was packaged aseptically, in screw-capped plastic cryovials. The product is provided frozen and should be Product Description: stored at -60°C or colder immediately upon arrival. For long- Bacteria Classification: Brucellaceae, Brucella term storage, the vapor phase of a liquid nitrogen freezer is Species: Brucella melitensis recommended. Freeze-thaw cycles should be avoided. Biotype/Biovar: 1 Strain: 16MΔvjbR Growth Conditions: Original Source: Brucella melitensis (B. melitensis), strain Note: Passage of this organism can result in the accumulation 16MΔvjbR
    [Show full text]
  • Research Collection
    Research Collection Doctoral Thesis Development and application of molecular tools to investigate microbial alkaline phosphatase genes in soil Author(s): Ragot, Sabine A. Publication Date: 2016 Permanent Link: https://doi.org/10.3929/ethz-a-010630685 Rights / License: In Copyright - Non-Commercial Use Permitted This page was generated automatically upon download from the ETH Zurich Research Collection. For more information please consult the Terms of use. ETH Library DISS. ETH NO.23284 DEVELOPMENT AND APPLICATION OF MOLECULAR TOOLS TO INVESTIGATE MICROBIAL ALKALINE PHOSPHATASE GENES IN SOIL A thesis submitted to attain the degree of DOCTOR OF SCIENCES of ETH ZURICH (Dr. sc. ETH Zurich) presented by SABINE ANNE RAGOT Master of Science UZH in Biology born on 25.02.1987 citizen of Fribourg, FR accepted on the recommendation of Prof. Dr. Emmanuel Frossard, examiner PD Dr. Else Katrin Bünemann-König, co-examiner Prof. Dr. Michael Kertesz, co-examiner Dr. Claude Plassard, co-examiner 2016 Sabine Anne Ragot: Development and application of molecular tools to investigate microbial alkaline phosphatase genes in soil, c 2016 ⃝ ABSTRACT Phosphatase enzymes play an important role in soil phosphorus cycling by hydrolyzing organic phosphorus to orthophosphate, which can be taken up by plants and microorgan- isms. PhoD and PhoX alkaline phosphatases and AcpA acid phosphatase are produced by microorganisms in response to phosphorus limitation in the environment. In this thesis, the current knowledge of the prevalence of phoD and phoX in the environment and of their taxonomic distribution was assessed, and new molecular tools were developed to target the phoD and phoX alkaline phosphatase genes in soil microorganisms.
    [Show full text]
  • 1 Preventing Laboratory-Acquired Brucellosis in the Era of MALDI-TOF
    Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 20 July 2020 doi:10.20944/preprints202007.0465.v1 1 Preventing Laboratory-acquired Brucellosis in the Era of MALDI-TOF Technology and Molecular Tests Pablo Yagupsky, MD Clinical Microbiology Laboratory, Soroka University Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel Manuscript word count: 4111 Abstract word count: 199 Corresponding Author: Pablo Yagupsky, Clinical Microbiology Laboratory, Soroka University Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel 84101. Phone number: (972) 506264359. Fax number: (972) 86403541. e-mail: [email protected] Abstract © 2020 by the author(s). Distributed under a Creative Commons CC BY license. Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 20 July 2020 doi:10.20944/preprints202007.0465.v1 2 Brucellosis is one of the most common etiologies of laboratory-acquired infections worldwide, and handling of living brucellae should be performed in a Class II biological safety cabinet. The low infecting dose, multiple portals of entry to the body, the great variety of potentially contaminated specimens, and the unspecific clinical manifestations of human infections facilitate the unintentional transmission of brucellae to laboratory personnel. Work accidents such as spillage of culture media cause only a small minority of exposures, whereas >80% of events result from unfamiliarity with the phenotypic features of the genus, misidentification of isolates, and unsafe laboratory practices such as aerosolization of bacteria and working on an open bench without protective goggles or gloves. Although the bacteriological diagnosis of brucellae by traditional methods is simple, the Gram stain and the biochemical profile of the organism, as determined by commercial kits, can be misleading, resulting in inadvertent exposure and contagion.
    [Show full text]
  • Horizontal Gene Transfer to a Defensive Symbiont with a Reduced Genome
    bioRxiv preprint doi: https://doi.org/10.1101/780619; this version posted September 24, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Horizontal gene transfer to a defensive symbiont with a reduced genome 2 amongst a multipartite beetle microbiome 3 Samantha C. Waterwortha, Laura V. Flórezb, Evan R. Reesa, Christian Hertweckc,d, 4 Martin Kaltenpothb and Jason C. Kwana# 5 6 Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin- 7 Madison, Madison, Wisconsin, USAa 8 Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution, 9 Johannes Gutenburg University, Mainz, Germanyb 10 Department of Biomolecular Chemistry, Leibniz Institute for Natural Products Research 11 and Infection Biology, Jena, Germanyc 12 Department of Natural Product Chemistry, Friedrich Schiller University, Jena, Germanyd 13 14 #Address correspondence to Jason C. Kwan, [email protected] 15 16 17 18 1 bioRxiv preprint doi: https://doi.org/10.1101/780619; this version posted September 24, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 19 ABSTRACT 20 The loss of functions required for independent life when living within a host gives rise to 21 reduced genomes in obligate bacterial symbionts. Although this phenomenon can be 22 explained by existing evolutionary models, its initiation is not well understood.
    [Show full text]
  • Taxonomic Hierarchy of the Phylum Proteobacteria and Korean Indigenous Novel Proteobacteria Species
    Journal of Species Research 8(2):197-214, 2019 Taxonomic hierarchy of the phylum Proteobacteria and Korean indigenous novel Proteobacteria species Chi Nam Seong1,*, Mi Sun Kim1, Joo Won Kang1 and Hee-Moon Park2 1Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea 2Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea *Correspondent: [email protected] The taxonomic hierarchy of the phylum Proteobacteria was assessed, after which the isolation and classification state of Proteobacteria species with valid names for Korean indigenous isolates were studied. The hierarchical taxonomic system of the phylum Proteobacteria began in 1809 when the genus Polyangium was first reported and has been generally adopted from 2001 based on the road map of Bergey’s Manual of Systematic Bacteriology. Until February 2018, the phylum Proteobacteria consisted of eight classes, 44 orders, 120 families, and more than 1,000 genera. Proteobacteria species isolated from various environments in Korea have been reported since 1999, and 644 species have been approved as of February 2018. In this study, all novel Proteobacteria species from Korean environments were affiliated with four classes, 25 orders, 65 families, and 261 genera. A total of 304 species belonged to the class Alphaproteobacteria, 257 species to the class Gammaproteobacteria, 82 species to the class Betaproteobacteria, and one species to the class Epsilonproteobacteria. The predominant orders were Rhodobacterales, Sphingomonadales, Burkholderiales, Lysobacterales and Alteromonadales. The most diverse and greatest number of novel Proteobacteria species were isolated from marine environments. Proteobacteria species were isolated from the whole territory of Korea, with especially large numbers from the regions of Chungnam/Daejeon, Gyeonggi/Seoul/Incheon, and Jeonnam/Gwangju.
    [Show full text]
  • Bacterial Diversity in Replicated Hydrogen Sulfide-Rich Streams
    Microbial Ecology https://doi.org/10.1007/s00248-018-1237-6 MICROBIOLOGY OF AQUATIC SYSTEMS Bacterial Diversity in Replicated Hydrogen Sulfide-Rich Streams Scott Hotaling1 & Corey R. Quackenbush1 & Julian Bennett-Ponsford1 & Daniel D. New2 & Lenin Arias-Rodriguez3 & Michael Tobler4 & Joanna L. Kelley1 Received: 13 January 2018 /Accepted: 23 July 2018 # Springer Science+Business Media, LLC, part of Springer Nature 2018 Abstract Extreme environments typically require costly adaptations for survival, an attribute that often translates to an elevated influence of habitat conditions on biotic communities. Microbes, primarily bacteria, are successful colonizers of extreme environments worldwide, yet in many instances, the interplay between harsh conditions, dispersal, and microbial biogeography remains unclear. This lack of clarity is particularly true for habitats where extreme temperature is not the overarching stressor, highlighting a need for studies that focus on the role other primary stressors (e.g., toxicants) play in shaping biogeographic patterns. In this study, we leveraged a naturally paired stream system in southern Mexico to explore how elevated hydrogen sulfide (H2S) influences microbial diversity. We sequenced a portion of the 16S rRNA gene using bacterial primers for water sampled from three geographically proximate pairings of streams with high (> 20 μM) or low (~ 0 μM) H2S concentrations. After exploring bacterial diversity within and among sites, we compared our results to a previous study of macroinvertebrates and fish for the same sites. By spanning multiple organismal groups, we were able to illuminate how H2S may differentially affect biodiversity. The presence of elevated H2S had no effect on overall bacterial diversity (p = 0.21), a large effect on community composition (25.8% of variation explained, p < 0.0001), and variable influence depending upon the group—whether fish, macroinvertebrates, or bacteria—being considered.
    [Show full text]
  • A Review of Brucellosis: a Recent Major Outbreak in Lebanon
    J Environ Sci Public Health 2021;5 (1):56-76 DOI: 10.26502/jesph.96120117 Review Article A Review of Brucellosis: A Recent Major Outbreak in Lebanon Alia Sabra1*, Bouchra el Masry2, Houssam Shaib2* 1One Health Program, Duke University, Durham, North Carolina, USA 2Department of Agriculture, Faculty of Agricultural and Food Sciences, American University of Beirut, Beirut, Lebanon *Corresponding Author: Houssam Shaib (PhD), Faculty of Agricultural and Food Sciences, American University of Beirut, Riad El Solh 1107-2020, PO Box 11-0236, Beirut, Lebanon, Tel: +961-1-350000; E-mail: [email protected] Alia Sabra, (MS in Environment, MS in Energy, Certificate in One Health), One Health Program, Duke University, Durham, North Carolina, E-mail: [email protected] Received: 08 January 2021; Accepted: 16 January 2021; Published: 11 February 2021 Citation: Alia Sabra, Bouchra el Masry, Houssam Shaib. A Review of Brucellosis: A Recent Major Outbreak in Lebanon. Journal of Environmental Science and Public Health 5 (2021): 56-76. Abstract with the Lebanese alimentary habits, brucellosis is Brucella infection remains the world’s most common commonly diagnosed in adults aged between 20 and bacterial zoonosis, with over half a million new cases 60 years old. This paper tailors the first annually, which brought renewed attention of this comprehensive One Health approach for the control neglected disease. This attention is highlighted in this of Brucellosis in Lebanon. Herein, a broad review to review manuscript, reporting worldwide outbreaks shed light on the complexity of Brucellosis and introducing the 5th major severely prominent discussing: the etiology; taxonomy; pathogenesis; worldwide outbreak since 2016 which occurred in epidemiology and geographic distribution of the Lebanon.
    [Show full text]
  • Supplementary Information
    K-mer similarity, networks of microbial genomes and taxonomic rank Guillaume Bernard, Paul Greenfield, Mark A. Ragan, Cheong Xin Chan. Supplementary Figures Legends # Supplementary Figure S1: P- network of prokaryote phyla using !" with k=25, based on rRNAs. Edges represent connections between isolates of two phyla. The node size is proportional to the number of isolates in a phylum. Distance threshold = 6. Supplementary Figure S2: PCA analysis performed on the raw data of the COG categories profile for each genus. Each phylum is color-coded. Supplementary Figure S3: PCA analysis performed on the raw data of the COG categories profile for each genus. Each genus is color-coded according to the number of isolates. Supplementary Figure S4: PCA analysis performed on the normalised counts of center-scaled COG categories. Each phylum is color-coded. Supplementary Tables Legends Supplementary Table S1: List of the 2785 isolates used in this analysis. Supplementary Table S2: Network analysis of the I-network for 2705 complete genomes of bacteria and archaea. Supplementary Table S3: Network analysis of the I-network for 2616 genomes of bacteria and archaea, with rRNA genes removed. Supplementary Table S4: Network analysis of the rRNA gene sequences I-network of 2616 bacterial and archaeal isolates. Supplementary Table S5: Network analysis of the plasmid genomes I-network of 921 bacterial and plasmid genomes. Supplementary Table S6: Statistics of core k-mers for 151 genera. Supplementary Table S7: COG category profiles for 16 phyla. 1 Figure S1
    [Show full text]
  • Effects of Salt Stress Levels on Nutritional Quality and Microorganisms of Alfalfa- Influenced Soil
    Effects of salt stress levels on nutritional quality and microorganisms of alfalfa- influenced soil Qiang Lu1, GenTu Ge1, DuoWen Sa1, ZhiJun Wang1, MeiLing Hou2 and Yu Shan Jia1 1 College of Grassland and Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region, China 2 College of Agriculture, Inner Mongolia University for Nationalities, Tongliao, Inner Mongolia Autonomous Region, China ABSTRACT Background. Globally, there is a large amount of salinized land. These soils have varying degrees of salt stress, causing ionic toxicity and osmotic stress on plants. However, it is not clear how different degrees of salt stress affect plant nutrients and microbial communities. Thus, a comprehensive understanding of plant major nutrients and microbial communities response to salt stress is desirable. Results. We analyzed the main nutrients of the salt-tolerant ZhongMu No. 3 alfalfa variety planted in a salt stress environment. In mild and moderate group, the protein content and fatty acid content of alfalfa were the highest, indicating the best nutritional value. The severe group of salt stress affected the growth and development of alfalfa, as manifested by a decrease in the nutritional quality of alfalfa. Pseudomonas and Sphingobacterium that from alfalfa stem and leaf endophytes also increased with an increase in salt stress. In contrast, Sphingomonas, Methylobacterium, and Rhizobium decrease with increasing salt stress. Methylobacterium and Rhizobium have extremely significant differences in response to salt stress, and Exiquobacterium also shows significant differences. Conclusions. Soil salinity would be an important factor beyond which alfalfa nutrient quality and microbial community structure change. This study identified key levels Submitted 14 November 2019 of salt stress that may affect the nutrient quality and microbial community structure.
    [Show full text]
  • Pyrosequencing Reveals a Shift in Symbiotic Bacteria Populations Across Life Stages of Bactrocera Dorsalis
    Pyrosequencing reveals a shift in symbiotic bacteria populations across life stages of Bactrocera dorsalis Awawing A. Andongma1, Lun Wan1, Yong-Cheng Dong1, Ping li2, Nicolas Desneux3, Jennifer A. White4, Chang-Ying Niu1* SUPPLEMENTARY INFORMATION Supplementary sheet 1 (S1): Bacteria Taxonomy and abundance in gut of different developmental stages of Bactrocera dorsalis OTU totalseq BDE BD1L BD3L BDP BDF BDM kingdom phylum class order family genus Otu001 18360 2723 2838 1441 6 4420 6932 Bacteria(100) Firmicutes(100) Bacilli(100) Lactobacillales(100) Enterococcaceae(100) unclassified(100) Otu002 6472 954 867 1437 3214 0 0 Bacteria(100) Proteobacteria(100) Betaproteobacteria(100) Burkholderiales(100) Comamonadaceae(100) Comamonas(100) Otu003 4890 897 1116 962 7 1671 237 Bacteria(100) Proteobacteria(100) Gammaproteobacteria(100) unclassified(100) unclassified(100) unclassified(100) Otu004 4048 655 807 745 1 1313 527 Bacteria(100) Proteobacteria(100) Deltaproteobacteria(100) Desulfovibrionales(94) unclassified(94) unclassified(94) Otu005 3977 737 759 1110 1286 63 22 Bacteria(100) Proteobacteria(100) Gammaproteobacteria(100) Enterobacteriales(100) Enterobacteriaceae(100) unclassified(91) Otu006 1367 245 230 218 0 285 389 Bacteria(100) Firmicutes(100) Bacilli(100) Lactobacillales(100) Streptococcaceae(100) Lactococcus(100) Otu007 1353 232 219 402 500 0 0 Bacteria(100) Proteobacteria(100) Betaproteobacteria(100) Burkholderiales(100) Comamonadaceae(100) unclassified(100) Otu008 1109 216 189 247 2 291 164 Bacteria(100) Bacteroidetes(100) Flavobacteria(100)
    [Show full text]