1 Metal and metalloid exposure and oxidative status in free- 2 living individuals of Myotis daubentonii 3 4 Sandra R. Ruiza*, Tapio Eevaa, Mirella Kanervaa, Anna Blomberga and Thomas 5 M. Lilleyb, c 6 a Department of Biology, University of Turku, 20014 Turku, Finland 7 b Institute of Integrative Biology, University of Liverpool, Liverpool, UK 8 c Finnish Museum of Natural History, University of Helsinki, Helsinki, Finland 9 *Corresponding author:
[email protected] 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Abstract 31 Metal elements, ubiquitous in the environment, can cause negative effects in long-lived 32 organisms even after low but prolonged exposure. Insectivorous bats living near metal emission 33 sources can be vulnerable to such contaminants. Although it is known that bats can 34 bioaccumulate metals, little information exists on the effects of metal elements on their 35 physiological status. For example, oxidative status markers are known to vary after 36 detoxification processes and immune reactions. Here, for two consecutive summers, we 37 sampled individuals from a natural population of the insectivorous bat, Myotis daubentonii, 38 inhabiting a site close to a metal emission source. We quantified metals and metalloids (As, 39 Ca, Cd, Co, Cu, Mn, Ni, Pb, Se, Zn) from individual fecal pellets. We measured enzymatic 40 antioxidants (GP, CAT, SOD), total glutathione (tGSH) and ratio between reduced and 41 oxidized glutathione (GSH:GSSG) from their red blood cells together with biometrics, 42 hematocrit and parasite prevalence. In general, metal concentrations in feces of M.