Anti-MGME1 Mouse mAb
Catalog # PTM-5474
General Information Images
Host species Mouse WB
Clonality Recombinant monoclonal Blocking buffer: 5% NFDM/TBST Clone number JMMR-1656 Primary ab dilution: 1:1000 Synonym C20orf72, DDK1 Primary ab incubation condition: 4°C overnight Immunogen UniProt MW (kDa) Applications Secondary ab: Goat Anti-Mouse IgG H&L species (HRP) Human Q9BQP7 39 WB Lysate: HeLa, HepG2, A549 Protein loading quantity: 20 μg Product Usage Information Exposure time: 60 s Predicted MW: 39 kDa Application Dilution Recommended species Observed MW: 35 kDa
WB 1:500-1:1000 Human Research Use
Properties For research use only, not for use in diagnostic procedures.
Storage Store at -20°C. Avoid freeze / thaw cycles.
Stability Stable for 12 months from date of receipt / reconstitution
Constituents PBS, Glycerol, BSA
Purity Protein G purified
Isotype IgG1/Kappa
Target Information
Function Metal-dependent single-stranded DNA (ssDNA)
exonuclease involved in mitochondrial genome
maintenance. Has preference for 5'-3' exonuclease
activity but is also capable of endoduclease activity on
linear substrates. Necessary for maintenance of proper
7S DNA levels. Probably involved in mitochondrial DNA
(mtDNA) repair, possibly via the processing of displaced
DNA containing Okazaki fragments during RNA-primed
DNA synthesis on the lagging strand or via processing of
DNA flaps during long-patch base excision repair.
Specifically binds 5-hydroxymethylcytosine (5hmC)-
containing DNA in stem cells.
Cellular location Mitochondrion
APPLICATIONS WB: Western blot IP: Immunoprecipitation CONTACT US China Global IHC-P: Immunohistochemistry-Paraffin ChIP: Chromatin Immunoprecipitation Tel: 400-100-1145 +1 312-802-6843 ICC/IF: Immunocytochemistry/Immunofluorescence FC: Flow Cytometry Email: [email protected] [email protected] Web: www.ptm-biolab.com.cn www.ptmbiolabs.com