Anti-MGME1 Mouse mAb

Catalog # PTM-5474

General Information Images

Host species Mouse WB

Clonality Recombinant monoclonal Blocking buffer: 5% NFDM/TBST Clone number JMMR-1656 Primary ab dilution: 1:1000 Synonym C20orf72, DDK1 Primary ab incubation condition: 4°C overnight Immunogen UniProt MW (kDa) Applications Secondary ab: Goat Anti-Mouse IgG H&L species (HRP) Human Q9BQP7 39 WB Lysate: HeLa, HepG2, A549 loading quantity: 20 μg Product Usage Information Exposure time: 60 s Predicted MW: 39 kDa Application Dilution Recommended species Observed MW: 35 kDa

WB 1:500-1:1000 Human Research Use

Properties For research use only, not for use in diagnostic procedures.

Storage Store at -20°C. Avoid freeze / thaw cycles.

Stability Stable for 12 months from date of receipt / reconstitution

Constituents PBS, Glycerol, BSA

Purity Protein G purified

Isotype IgG1/Kappa

Target Information

Function Metal-dependent single-stranded DNA (ssDNA)

exonuclease involved in mitochondrial genome

maintenance. Has preference for 5'-3' exonuclease

activity but is also capable of endoduclease activity on

linear substrates. Necessary for maintenance of proper

7S DNA levels. Probably involved in mitochondrial DNA

(mtDNA) repair, possibly via the processing of displaced

DNA containing Okazaki fragments during RNA-primed

DNA synthesis on the lagging strand or via processing of

DNA flaps during long-patch base excision repair.

Specifically binds 5-hydroxymethylcytosine (5hmC)-

containing DNA in stem cells.

Cellular location

APPLICATIONS WB: Western blot IP: Immunoprecipitation CONTACT US China Global IHC-P: Immunohistochemistry-Paraffin ChIP: Chromatin Immunoprecipitation Tel: 400-100-1145 +1 312-802-6843 ICC/IF: Immunocytochemistry/Immunofluorescence FC: Flow Cytometry Email: [email protected] [email protected] Web: www.ptm-biolab.com.cn www.ptmbiolabs.com