Aeromonas Hydrophila Subsp. Dhakensis
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Delineation of Aeromonas Hydrophila Pathotypes by Dectection of Putative Virulence Factors Using Polymerase Chain Reaction and N
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by DigitalCommons@Kennesaw State University Kennesaw State University DigitalCommons@Kennesaw State University Master of Science in Integrative Biology Theses Biology & Physics Summer 7-20-2015 Delineation of Aeromonas hydrophila Pathotypes by Dectection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay John Metz Kennesaw State University, [email protected] Follow this and additional works at: http://digitalcommons.kennesaw.edu/integrbiol_etd Part of the Integrative Biology Commons Recommended Citation Metz, John, "Delineation of Aeromonas hydrophila Pathotypes by Dectection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay" (2015). Master of Science in Integrative Biology Theses. Paper 7. This Thesis is brought to you for free and open access by the Biology & Physics at DigitalCommons@Kennesaw State University. It has been accepted for inclusion in Master of Science in Integrative Biology Theses by an authorized administrator of DigitalCommons@Kennesaw State University. For more information, please contact [email protected]. Delineation of Aeromonas hydrophila Pathotypes by Detection of Putative Virulence Factors using Polymerase Chain Reaction and Nematode Challenge Assay John Michael Metz Submitted in partial fulfillment of the requirements for the Master of Science Degree in Integrative Biology Thesis Advisor: Donald J. McGarey, Ph.D Department of Molecular and Cellular Biology Kennesaw State University ABSTRACT Aeromonas hydrophila is a Gram-negative, bacterial pathogen of humans and other vertebrates. Human diseases caused by A. hydrophila range from mild gastroenteritis to soft tissue infections including cellulitis and acute necrotizing fasciitis. When seen in fish it causes dermal ulcers and fatal septicemia, which are detrimental to aquaculture stocks and has major economic impact to the industry. -
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Zagazig J. Agric. Res., Vol. 47 No. (1) 2020 179 -179197 Biotechnology Research http:/www.journals.zu.edu.eg/journalDisplay.aspx?Journalld=1&queryType=Master ISOLATION OF Aeromonas BACTERIOPHAGE AvF07 FROM FISH AND ITS APPLICATION FOR BIOLOGICAL CONTROL OF MULTIDRUG RESISTANT LOCAL Aeromonas veronii AFs 2 Nahed A. El-Wafai *, Fatma I. El-Zamik, S.A.M. Mahgoub and Alaa M.S. Atia Agric. Microbiol. Dept., Fac. Agric., Zagazig Univ., Egypt Received: 25/09/2019 ; Accepted: 25/11/2019 ABSTRACT: Aeromonas isolates from Nile tilapia fish, fish ponds and River water were identified as well as their bacteriophage specific. Also evaluation of antibacterial effect of both nanoparticles and phage therapy against the pathogenic Aeromonas veronii AFs 2. Differentiation of Aeromonas spp. was done on the basis of 25 different biochemical tests and confirmed by sequencing of 16s rRNA gene as (A. caviae AFg, A. encheleia AWz, A. molluscorum AFm, A. salmonicida AWh, A. veronii AFs 2, A. veronii bv. veronii AFi). All of the six Aeromonas strains were resistant to β-actam (amoxicillin/ lavulanic acid) antibiotics. However, the resistance to other antibiotics was variable. All Aeromonas strains were found to be resistant to ampicillin, cephalexin, cephradine, amoxicillin/clavulanic acid, rifampin and cephalothin. Sensitivity of 6 Aeromonas strains raised against 7 concentrations of chitosan nanoparticles. Using well diffusion method spherically shaped silver nanoparticles AgNPs with an average size of ~ 20 nm, showed a great antimicrobial activity against A. veronii AFs 2 and five more strains of Aeromonas spp. At the concentration of 20, 24, 32 and 40 µg/ml. Thermal inactivation point was 84 oC for phage AvF07 which was sensitive to storage at 4 oC compared with the storage at - o 20 C. -
An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity
microorganisms Review An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity Ana Fernández-Bravo and Maria José Figueras * Unit of Microbiology, Department of Basic Health Sciences, Faculty of Medicine and Health Sciences, IISPV, University Rovira i Virgili, 43201 Reus, Spain; [email protected] * Correspondence: mariajose.fi[email protected]; Tel.: +34-97-775-9321; Fax: +34-97-775-9322 Received: 31 October 2019; Accepted: 14 January 2020; Published: 17 January 2020 Abstract: The genus Aeromonas belongs to the Aeromonadaceae family and comprises a group of Gram-negative bacteria widely distributed in aquatic environments, with some species able to cause disease in humans, fish, and other aquatic animals. However, bacteria of this genus are isolated from many other habitats, environments, and food products. The taxonomy of this genus is complex when phenotypic identification methods are used because such methods might not correctly identify all the species. On the other hand, molecular methods have proven very reliable, such as using the sequences of concatenated housekeeping genes like gyrB and rpoD or comparing the genomes with the type strains using a genomic index, such as the average nucleotide identity (ANI) or in silico DNA–DNA hybridization (isDDH). So far, 36 species have been described in the genus Aeromonas of which at least 19 are considered emerging pathogens to humans, causing a broad spectrum of infections. Having said that, when classifying 1852 strains that have been reported in various recent clinical cases, 95.4% were identified as only four species: Aeromonas caviae (37.26%), Aeromonas dhakensis (23.49%), Aeromonas veronii (21.54%), and Aeromonas hydrophila (13.07%). -
Salmonicida from Aeromonas Bestiarum
RESEARCH ARTICLE INTERNATIONAL MICROBIOLOGY (2005) 8:259-269 www.im.microbios.org Antonio J. Martínez-Murcia1* Phenotypic, genotypic, and Lara Soler2 Maria José Saavedra1,3 phylogenetic discrepancies Matilde R. Chacón Josep Guarro2 to differentiate Aeromonas Erko Stackebrandt4 2 salmonicida from María José Figueras Aeromonas bestiarum 1Molecular Diagnostics Center, and Univ. Miguel Hernández, Orihuela, Alicante, Spain 2Microbiology Unit, Summary. The taxonomy of the “Aeromonas hydrophila” complex (compris- Dept. of Basic Medical Sciences, ing the species A. hydrophila, A. bestiarum, A. salmonicida, and A. popoffii) has Univ. Rovira i Virgili, Reus, Spain been controversial, particularly the relationship between the two relevant fish 3Dept. of Veterinary Sciences, pathogens A. salmonicida and A. bestiarum. In fact, none of the biochemical tests CECAV-Univ. of Trás-os-Montes evaluated in the present study were able to separate these two species. One hun- e Alto Douro, Vila Real, Portugal dred and sixteen strains belonging to the four species of this complex were iden- 4DSMZ-Deutsche Sammlung von tified by 16S rDNA restriction fragment length polymorphism (RFLP). Mikroorganismen und Zellkulturen Sequencing of the 16S rDNA and cluster analysis of the 16S–23S intergenic GmbH, Braunschwieg, Germany spacer region (ISR)-RFLP in selected strains of A. salmonicida and A. bestiarum indicated that the two species may share extremely conserved ribosomal operons and demonstrated that, due to an extremely high degree of sequence conserva- tion, 16S rDNA cannot be used to differentiate these two closely related species. Moreover, DNA–DNA hybridization similarity between the type strains of A. salmonicida subsp. salmonicida and A. bestiarum was 75.6 %, suggesting that Received 8 September 2005 they may represent a single taxon. -
Succession of Lignocellulolytic Bacterial Consortia Bred Anaerobically from Lake Sediment
bs_bs_banner Succession of lignocellulolytic bacterial consortia bred anaerobically from lake sediment Elisa Korenblum,*† Diego Javier Jimenez and A total of 160 strains was isolated from the enrich- Jan Dirk van Elsas ments. Most of the strains tested (78%) were able to Department of Microbial Ecology,Groningen Institute for grow anaerobically on carboxymethyl cellulose and Evolutionary Life Sciences,University of Groningen, xylan. The final consortia yield attractive biological Groningen,The Netherlands. tools for the depolymerization of recalcitrant ligno- cellulosic materials and are proposed for the produc- tion of precursors of biofuels. Summary Anaerobic bacteria degrade lignocellulose in various Introduction anoxic and organically rich environments, often in a syntrophic process. Anaerobic enrichments of bacte- Lignocellulose is naturally depolymerized by enzymes of rial communities on a recalcitrant lignocellulose microbial communities that develop in soil as well as in source were studied combining polymerase chain sediments of lakes and rivers (van der Lelie et al., reaction–denaturing gradient gel electrophoresis, 2012). Sediments in organically rich environments are amplicon sequencing of the 16S rRNA gene and cul- usually waterlogged and anoxic, already within a cen- turing. Three consortia were constructed using the timetre or less of the sediment water interface. There- microbiota of lake sediment as the starting inoculum fore, much of the organic detritus is probably degraded and untreated switchgrass (Panicum virgatum) (acid by anaerobic processes in such systems (Benner et al., or heat) or treated (with either acid or heat) as the 1984). Whereas fungi are well-known lignocellulose sole source of carbonaceous compounds. Addition- degraders in toxic conditions, due to their oxidative ally, nitrate was used in order to limit sulfate reduc- enzymes (Wang et al., 2013), in anoxic environments tion and methanogenesis. -
Identification and Characterization of Aeromonas Species Isolated from Ready- To-Eat Lettuce Products
Master's thesis Noelle Umutoni Identification and Characterization of Aeromonas species isolated 2019 from ready-to-eat lettuce Master's thesis products. Noelle Umutoni NTNU May 2019 Norwegian University of Science and Technology Faculty of Natural Sciences Department of Biotechnology and Food Science Identification and Characterization of Aeromonas species isolated from ready- to-eat lettuce products. Noelle Umutoni Food science and Technology Submission date: May 2019 Supervisor: Lisbeth Mehli Norwegian University of Science and Technology Department of Biotechnology and Food Science Preface This thesis covers 45 ECTS-credits and was carried out as part of the M. Sc. programme for Food and Technology at the institute of Biotechnology and Food Science, faculty of natural sciences at the Norwegian University of Science and Technology in Trondheim in spring 2019. First, I would like to express my gratitude to my main supervisor Associate professor Lisbeth Mehli. Thank you for the laughs, advice, and continuous encouragement throughout the project. Furthermore, appreciations to PhD Assistant professor Gunn Merethe Bjørge Thomassen for valuable help in the lab. Great thanks to my family and friends for their patience and encouragement these past years. Thank you for listening, despite not always understanding the context of my studies. A huge self-five to myself, for putting in the work. Finally, a tremendous thank you to Johan – my partner in crime and in life. I could not have done this without you. You kept me fed, you kept sane. I appreciate you from here to eternity. Mama, we made it! 15th of May 2019 Author Noelle Umutoni I Abstract Aeromonas spp. -
Aeromonas Strains
A Rapid MALDI-TOF MS Identification Database at Genospecies Level for Clinical and Environmental Aeromonas Strains Cinzia Benagli1*, Antonella Demarta1, AnnaPaola Caminada1, Dominik Ziegler2, Orlando Petrini1, Mauro Tonolla1,3 1 Institute of Microbiology, Bellinzona, Switzerland, 2 Mabritec AG, Riehen, Switzerland, 3 Microbial Ecology, Microbiology Unit, Plant Biology Department University of Geneva, Gene`ve, Switzerland Abstract The genus Aeromonas has undergone a number of taxonomic and nomenclature revisions over the past 20 years, and new (sub)species and biogroups are continuously described. Standard identification methods such as biochemical characterization have deficiencies and do not allow clarification of the taxonomic position. This report describes the development of a matrix-assisted laser desorption/ionisation–time of flight mass spectrometry (MALDI-TOF MS) identification database for a rapid identification of clinical and environmental Aeromonas isolates. Citation: Benagli C, Demarta A, Caminada A, Ziegler D, Petrini O, et al. (2012) A Rapid MALDI-TOF MS Identification Database at Genospecies Level for Clinical and Environmental Aeromonas Strains. PLoS ONE 7(10): e48441. doi:10.1371/journal.pone.0048441 Editor: Mikael Skurnik, University of Helsinki, Finland Received May 10, 2012; Accepted September 25, 2012; Published October 31, 2012 Copyright: ß 2012 Benagli et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: No current external funding sources for this study. Competing Interests: The authors have read the journal’s policy and have the following conflicts: Dominik Ziegler is employed by Mabritec AG. -
Investigation of the Virulence and Genomics of Aeromonas Salmonicida Strains Isolated from Human Patients T
Infection, Genetics and Evolution 68 (2019) 1–9 Contents lists available at ScienceDirect Infection, Genetics and Evolution journal homepage: www.elsevier.com/locate/meegid Research paper Investigation of the virulence and genomics of Aeromonas salmonicida strains isolated from human patients T Antony T. Vincenta,1, Ana Fernández-Bravob,1, Marta Sanchisb, Emilio Mayayob,c, ⁎⁎ ⁎ María Jose Figuerasb, , Steve J. Charettea, a Université Laval, Quebec City, QC, Canada b Universitat Rovira i Virgili, IISPV, Reus, Spain c University Hospital Joan XIII, Tarragona, Spain ARTICLE INFO ABSTRACT Keywords: The bacterium Aeromonas salmonicida is known since long time as a major fish pathogen unable to grow at 37 °C. Aeromonas salmonicida However, some cases of human infection by putative mesophilic A. salmonicida have been reported. The goal of Infection the present study is to examine two clinical cases of human infection by A. salmonicida in Spain and to in- Necrotizing fasciitis vestigate the pathogenicity in mammals of selected mesophilic A. salmonicida strains. An evaluation of the pa- Pathogenicity thogenicity in a mouse model of clinical and environmental A. salmonicida strains was performed. The genomes Type III secretion systems of the strains were sequenced and analyzed in order to find the virulence determinants of these strains. The Whole genome sequencing experimental infection in mice showed a gradient in the virulence of these strains and that some of them can cause necrotizing fasciitis and tissue damage in the liver. In addition to demonstrating significant genomic diversity among the strains studied, bioinformatics analyses permitted also to shed light on crucial elements for the virulence of the strains, like the presence of a type III secretion system in the one that caused the highest mortality in the experimental infection. -
Caracterización De Bacterias Aeromonadales Móviles Aisladas De Peces Cultivados En Uruguay
UNIVERSIDAD DE LA REPÚBLICA FACULTAD DE VETERINARIA Programa de Posgrados CARACTERIZACIÓN DE BACTERIAS AEROMONADALES MÓVILES AISLADAS DE PECES CULTIVADOS EN URUGUAY Alejandro Perretta TESIS DE MAESTRÍA EN SALUD ANIMAL URUGUAY 2016 i ii iii UNIVERSIDAD DE LA REPÚBLICA FACULTAD DE VETERINARIA Programa de Posgrados CARACTERIZACIÓN DE BACTERIAS AEROMONADALES MÓVILES AISLADAS DE PECES CULTIVADOS EN URUGUAY Alejandro Perretta _________________________ _________________________ Dr. Pablo Zunino Dra. Karina Antúnez Director de Tesis Codirectora de Tesis 2016 iv INTEGRACIÓN DEL TRIBUNAL DE DEFENSA DE TESIS Claudia Piccini; MS, PhD Departamento de Microbiología Instituto de Investigaciones Biológicas "Clemente Estable"- Ministerio de Educación y Cultura - Uruguay Laura Bentancor; MS, PhD Departamento de Bacteriología y Virología Instituto de Higiene - Facultad de Medicina Universidad de la República - Uruguay Martín Fraga; MS, PhD Plataforma de Salud Animal Instituto de Investigaciones Agropecuarias - Uruguay 2016 v vi vii DEDICATORIA …para Federíco, Anaclara y Valeria viii AGRADECIMIENTOS - A Pablo Zunino y Karina Antúnez, gracias por compartir el conocimiento conmigo, abrirme las puertas del IIBCE para poder llevar a cabo este trabajando y por la paciencia y el apoyo en todo momento. - A Daniel Carnevia por el apoyo contínuo y la colaboración brindada en cada uno de mis emprendimientos. - A todo el personal docente y no docente del Instituto de Investigaciones Pesqueras de la Facultad de Veterinaria por tantos años de apoyo y colaboración. - A Belén Branchiccela y todo el personal del Departamento de Microbiología del IIBCE por el apoyo y la colaboración en todas las tareas llevadas a cabo allí. - A Rodrigo Puentes, Uruguaysito Benavides y todo el personal del Área Inmunología de la Facultad de Veterinaria por la colaboración con las taréas de biología molecular llevadas a cabo. -
Malaysian Journal of Microbiology, Vol 12(3) 2016, Pp
Malaysian Journal of Microbiology, Vol 12(3) 2016, pp. 191-198 http://dx.doi.org/10.21161/mjm.77915 Malaysian Journal of Microbiology Published by Malaysian Society for Microbiology (In since 2011) Evaluation of identification techniques for the fish pathogen, Aeromonas hydrophila, from Indonesia Diah Kusumawaty 1,2, Adi Pancoro1, I. Nyoman P. Aryantha1 and Sony Suhandono1* 1School of Life Sciences and Technology, Bandung Institute of Technology, Indonesia. Jl. Ganesha No. 10, Bandung 40132 Indonesia. 2Dept. of Biology Education, Indonesia University of Education, Jl. Dr. Setiabudi No 229 Bandung 40154 Indonesia. Email: [email protected] Received 30 September 2015; Received in revised form 9 December 2015; Accepted 9 December 2015 ABSTRACT Aims: This study evaluated the accuracy of three methods used in the identification of Aeromonas hydrophila, a Gram- negative bacterium found in warm aquatic environments. A. hydrophila samples from Indonesia were tested using (a) SNI 7303, developed by the Indonesian government, (b) the method of Dorsch and (c) the method of Cascón. The results obtained were compared to that of the gold standard method, which used 16S rDNA sequences. Methodology and results: Based on the Indonesian government standard identification method SNI7303, we identified 56 out of 95 samples as A. hydrophila. The samples were then screened using the PCR amplification approach developed by Dorsch and Cascón. Of the 56 samples, only 20 samples were found to be positive by either the Dorsch or Cascón methods. DNA from these 20 samples was amplified using common 16S rDNA primers and the sequences compared with available 16S rDNA sequences from the GenBank. -
Pathogenicity and Antibiotics Sensitivity Profile of Aeromonas Bestiarum Used in Experimental Infection of Different Developmental Stages of Clarias Gariepinus
www.kosmospublishers.com [email protected] Research Article Journal of Aquaculture, Marine Biology & Ecology JAMBE-103 Pathogenicity and Antibiotics Sensitivity Profile of Aeromonas Bestiarum Used In Experimental Infection of Different Developmental Stages of Clarias Gariepinus Olakunle S Tiamiyu1*, Oladosu G.A1, Anifowose O.R1, Ajayi O.L2 1University of Ibadan, Nigeria 2Federal University of Agriculture Abeokuta, Nigeria Received Date: February 27, 2020; Accepted Date: March 4, 2020; Published Date: March 13, 2020 *Corresponding author: Olakunle S Tiamiyu, University of Ibadan, Nigeria. Tel: +2348060715385, +2348098715385; Email: [email protected] Summary mortality in the treated fry, juveniles and post-juveniles respectively, while the mortality rate of untreated but infected Motile Aeromonas Septicaemia (MAS) known to be the group (control) were 40%, 42% and 42% for fry, juveniles and commonest bacterial infection of cultured fish is mostly post-juveniles fish respectively. This shows Aeromonas ascribed to Aeromonas hydrophila. This study was therefore bestiarum causes high mortality in fry, juvenile and post- conducted to determine the pathogenicity of Aeromonas juvenile of Clarias gariepinus. It's however sensitive to bestiarum in fry, juvenile and post-juvenile of Clarias Enrofloxacin and Gentamicin which can be used for treatment gariepinus, and evaluate the antibiotic sensitivity profile of the of infection by Aeromonas bestiarum for now. organism for effective control. Aeromonas bestiarum was isolated from dead fry in Ijebu Ode. The organism was Introduction characterized and used for this study. Two-hundred apparently healthy fry collected from a commercial hatchery were African catfish also called African sharp tooth catfish, randomly divided into four experimental groups of 50 fry. -
Aeromonas Spp
UNIVERSIDAD AUTÓNOMA DEL ESTADO DE MÉXICO MAESTRÍA Y DOCTORADO EN CIENCIAS AGROPECUARIAS Y RECURSOS NATURALES CARACTERIZACIÓN FENOTÍPICA Y GENOTÍPICA DE AISLAMIENTOS DE Aeromonas spp. OBTENIDOS DE TRUCHA ARCOÍRIS ( Oncorhynchus mykiss ) T E S I S QUE PARA OBTENER EL GRADO DE DOCTOR EN CIENCIAS AGROPECUARIAS Y RECURSOS NATURALES PRESENTA: M. en C. VICENTE VEGA SÁNCHEZ El Cerrillo Piedras Blancas, Toluca, Estado de México. Agosto 2014 RESUMEN El desarrollo de cultivos en condiciones intensivas conlleva el riesgo de aparición de enfermedades infecciosas, las cuales pued en generar importantes pérdidas económicas; las infecciones causadas por bacterias del género Aeromonas tienen una distribución mundial, particularmente en patologías de peces en numerosos países. En México se ha reportado una prevalencia del 48.77% para este género bacteriano . El objetivo del presente trabajo fue identificar al nivel de especie, aislamientos de Aeromonas spp. utilizando métodos moleculares, conocer el patrón de sensibilidad antimicrobiana a diversos antimicrobianos e identificar genes que codifican para la resistencia antimicrobiana en los aislamientos. Las especies identificadas fueron A. veronii (29.2%), A. bestiarum (20.8%), A. hydrophila (16.7%), A. sobria (10.4%), A. media (8.3%), A. popoffii (6.2%), A. allosaccharophila (2.1%), A. caviae (2.1%), A. salmonicida (2.1%) y “ Aeromonas lusitana” (2.1%), la cual está pendiente su reporte como especie nueva. Se observó una correcta identificación entre métodos bioquímicos y secuenciación de genes housekeeping del 12% (6/50) y del 70% (35/55) entre la identificación por RFLP del gen 16S DNAr y genes que codifican para proteínas esenciales o housekeeping . Se detectó la presencia de patrones atípicos obtenidos por RFLP del gen 16S DNAr los cuales complican la correcta identificación.