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Targeted gene sequencing and selection detection in ex situ collections of Brighamia insignis Christina Shehata1, Lauren Audi1,2, Hilary Noble2, Colby Witherup1,2, Jeremie Fant2, Norm Wickett2 1Northwestern University, Evanston, IL 60201; 2Chicago Botanic Garden, Glencoe, IL 60022 Introduction Results B_insignis_107_BI−001 Discussion B_insignis_UNK_BI−005 B_insignis_1_BI−006 B_insignis_132_BI−007 Brighamia insignis (family: Campanulaceae, subfamily: Lobelioideae) is a B_insignis_255_BI−014 B_insignis_255_BI−015 B_insignis_255_BI−020 While 206 genes were recovered during assembly, some genes were B_insignis_255_BI−031 B_insignis_255_BI−032 B_insignis_UNK_BI−034 B_insignis_565_BI−052 B_insignis_92_BI−073 species of caudiciform succulent endemic to Hawaii. Brighamia insignis is B_insignis_256_BI−077 B_insignis_wild_BI−083 B_insignis_wild_BI−084 not captured by the Campanulaceae probes in any of 83 samples (Figure B_insignis_wild_BI−087 B_insignis_wild_BI−095 B_insignis_wild_BI−097 B_insignis_UNK_BI−099 B_insignis_wild_BI−104 extinct in the wild, due to habitat loss, small population sizes, and the loss B_insignis_257_BI−107 B_insignis_1002_BI−109 B_insignis_106_BI−110 2). The probes were designed from Campanula erinus, a relatively distant B_insignis_UNK_BI−112 B_insignis_wild_BI−113 B_insignis_UNK_BI−124 1 B_insignis_UNK_BI−129 B_insignis_UNK_BI−131 of its native pollinator, Tinostoma smaragditis . Fortunately, due to their B_insignis_UNK_BI−1367 B_insignis_UNK_BI−139 B_insignis_MULT21_BI−153 species from Brighamia insignis, possibly explaining the lower success of B_insignis_UNK_BI−160 B_insignis_UNK_BI−201 B_insignis_UNK_BI−208 B_insignis_UNK_BI−211 B_insignis_UNK_BI−212 novel morphology, Brighamia insignis have been cultivated as ornamental B_insignis_UNK_BI−215 B_insignis_UNK_BI−218 B_insignis_UNK_BI−231 target capture for some particular probes. The overall diversity among B_insignis_UNK_BI−237 B_insignis_UNK_BI−251 B_insignis_wild_BI−252 B_insignis_wild_BI−253 B_rockii_wild_BR−008 plants and still exist in ex situ collections around the world. B_rockii_wild_BR−016 B_rockii_wild_BR−020 B_rockii_97_BR−077 B_rockii_wild_BR−098 the samples of Brighamia insignis was greater than expected, with B_rockii_wild_BR−099 B_rockii_UNK_BR−100 B_rockii_UNK_BR−101 B_rockii_UNK_HKM002 Genomic data obtained via next-generation B_insignis_UNK_HKM003 D_rhytidosperma_UNK_HKM004 B_rockii_wild_HW012 Cl_fauriei_wild_HW016 multiple points of divergence observed on the population tree along the Cl_fauriei_wild_HW025 T_kauaiensiswild_HW027 T_kauaiensiswild_HW031 D_rhytidosperma_UNK_HW040 sequencing methods provide valuable information D_rhytidosperma_UNK_HW041 D_kauensis_wild_HW042 D_kauensis_wild_HW043 Cy_pseudofauriei_wild_HW044 branch leading to the populations of Brighamia insignis (Figure 2). Cy_pseudofauriei_wild_HW046 Cy_leptostegia_wild_HW048 Cy_leptostegia_wild_HW049 Cy_leptostegia_wild_HW050 about putatively expressed regions of the genome. Cy_hardyi_wild_HW051 Cy_fissa_wild_HW052 Cy_fissa_wild_HW053 Cy_fissa_wild_HW054 93 genes were found to have an LSDnorm value greater than 0 (Figure Cy_fissa_wild_HW055 Cl_fauriei_wild_HW059 Cy_hardyi_wild_HW061 B_rockii_wild_HW063 B_rockii_wild_HW064 Identifying those genes under natural selection can D_waianaeensis_UNK_LY002 B_rockii_UNK_ML002 B_rockii_UNK_ML005 3), suggesting that these genes are adaptive candidates within the D_kauensis_UNK_NT005 B_insignis_UNK_QKP005 help conservation scientists understand the sampled populations of Brighamia insignis. While traditional population scaffold_XAEC_2031402 scaffold_XAEC_2009701 scaffold_XAEC_2085436 scaffold_XAEC_2084760 scaffold_XAEC_2014344 scaffold_XAEC_2082868 scaffold_XAEC_2083385 scaffold_XAEC_2085304 scaffold_XAEC_2014228 scaffold_XAEC_2012381 scaffold_XAEC_2013546 scaffold_XAEC_2010914 scaffold_XAEC_2085985 scaffold_XAEC_2072819 scaffold_XAEC_2005335 scaffold_XAEC_2084276 scaffold_XAEC_2085700 scaffold_XAEC_2082107 scaffold_XAEC_2081424 scaffold_XAEC_2085904 scaffold_XAEC_2006755 scaffold_XAEC_2017460 scaffold_XAEC_2000424 scaffold_XAEC_2086239 scaffold_XAEC_2082050 scaffold_XAEC_2085725 scaffold_XAEC_2009935 scaffold_XAEC_2011064 scaffold_XAEC_2001544 scaffold_XAEC_2085482 scaffold_XAEC_2079785 scaffold_XAEC_2016261 scaffold_XAEC_2081872 scaffold_XAEC_2080877 scaffold_XAEC_2011700 scaffold_XAEC_2014153 scaffold_XAEC_2083592 scaffold_XAEC_2014329 scaffold_XAEC_2006092 scaffold_XAEC_2080494 scaffold_XAEC_2085496 scaffold_XAEC_2078669 scaffold_XAEC_2001114 scaffold_XAEC_2071303 scaffold_XAEC_2086058 scaffold_XAEC_2070149 scaffold_XAEC_2003396 scaffold_XAEC_2079367 scaffold_XAEC_2005232 scaffold_XAEC_2082937 scaffold_XAEC_2015246 scaffold_XAEC_2082812 scaffold_XAEC_2081103 scaffold_XAEC_2017079 scaffold_XAEC_2005196 scaffold_XAEC_2017875 scaffold_XAEC_2013884 scaffold_XAEC_2017561 scaffold_XAEC_2001477 scaffold_XAEC_2080606 scaffold_XAEC_2082141 scaffold_XAEC_2009747 scaffold_XAEC_2014175 scaffold_XAEC_2009451 scaffold_XAEC_2081270 scaffold_XAEC_2084298 scaffold_XAEC_2082941 scaffold_XAEC_2064325 scaffold_XAEC_2080433 scaffold_XAEC_2013530 scaffold_XAEC_2004450 scaffold_XAEC_2083526 scaffold_XAEC_2015208 scaffold_XAEC_2006174 scaffold_XAEC_2057767 scaffold_XAEC_2017553 scaffold_XAEC_2001888 scaffold_XAEC_2001550 scaffold_XAEC_2000846 scaffold_XAEC_2011036 scaffold_XAEC_2037036 scaffold_XAEC_2083253 scaffold_XAEC_2017893 scaffold_XAEC_2077649 scaffold_XAEC_2084810 scaffold_XAEC_2006412 scaffold_XAEC_2084741 scaffold_XAEC_2009073 scaffold_XAEC_2017521 scaffold_XAEC_2084670 scaffold_XAEC_2006695 scaffold_XAEC_2085611 scaffold_XAEC_2086014 scaffold_XAEC_2079951 scaffold_XAEC_2083474 scaffold_XAEC_2081492 scaffold_XAEC_2079547 scaffold_XAEC_2017491 scaffold_XAEC_2083079 scaffold_XAEC_2017499 scaffold_XAEC_2017095 scaffold_XAEC_2084829 scaffold_XAEC_2085928 scaffold_XAEC_2083788 scaffold_XAEC_2012087 scaffold_XAEC_2030622 scaffold_XAEC_2011401 scaffold_XAEC_2082878 scaffold_XAEC_2083607 scaffold_XAEC_2012769 scaffold_XAEC_2083953 scaffold_XAEC_2085250 scaffold_XAEC_2016681 scaffold_XAEC_2079242 scaffold_XAEC_2084852 scaffold_XAEC_2085290 scaffold_XAEC_2084227 scaffold_XAEC_2014903 scaffold_XAEC_2009007 scaffold_XAEC_2083270 scaffold_XAEC_2074174 variation that influences the fitness of individuals. XAEC_scaffold_XAEC_2017819 XAEC_scaffold_XAEC_2017706 XAEC_scaffold_XAEC_2083730 XAEC_scaffold_XAEC_2082801 XAEC_scaffold_XAEC_2083964 XAEC_scaffold_XAEC_2017474 XAEC_scaffold_XAEC_2082687 XAEC_scaffold_XAEC_2017899 XAEC_scaffold_XAEC_2003001 XAEC_scaffold_XAEC_2085914 XAEC_scaffold_XAEC_2016820 XAEC_scaffold_XAEC_2084049 XAEC_scaffold_XAEC_2084620 XAEC_scaffold_XAEC_2083751 XAEC_scaffold_XAEC_2007962 XAEC_scaffold_XAEC_2082878 XAEC_scaffold_XAEC_2080889 XAEC_scaffold_XAEC_2084899 XAEC_scaffold_XAEC_2006461 XAEC_scaffold_XAEC_2081183 XAEC_scaffold_XAEC_2004648 XAEC_scaffold_XAEC_2014207 XAEC_scaffold_XAEC_2082730 XAEC_scaffold_XAEC_2085672 XAEC_scaffold_XAEC_2011632 XAEC_scaffold_XAEC_2017888 XAEC_scaffold_XAEC_2085349 XAEC_scaffold_XAEC_2084964 XAEC_scaffold_XAEC_2016838 XAEC_scaffold_XAEC_2081064 XAEC_scaffold_XAEC_2084968 XAEC_scaffold_XAEC_2081539 XAEC_scaffold_XAEC_2083571 XAEC_scaffold_XAEC_2007280 XAEC_scaffold_XAEC_2080356 XAEC_scaffold_XAEC_2001087 XAEC_scaffold_XAEC_2083714 XAEC_scaffold_XAEC_2083540 XAEC_scaffold_XAEC_2082861 XAEC_scaffold_XAEC_2017484 XAEC_scaffold_XAEC_2013983 XAEC_scaffold_XAEC_2084272 XAEC_scaffold_XAEC_2081317 XAEC_scaffold_XAEC_2084904 XAEC_scaffold_XAEC_2085000 XAEC_scaffold_XAEC_2084767 XAEC_scaffold_XAEC_2014103 XAEC_scaffold_XAEC_2015748 XAEC_scaffold_XAEC_2084975 XAEC_scaffold_XAEC_2083564 XAEC_scaffold_XAEC_2084044 XAEC_scaffold_XAEC_2082272 XAEC_scaffold_XAEC_2084608 XAEC_scaffold_XAEC_2016994 XAEC_scaffold_XAEC_2014535 XAEC_scaffold_XAEC_2082633 XAEC_scaffold_XAEC_2083385 XAEC_scaffold_XAEC_2086004 XAEC_scaffold_XAEC_2086273 XAEC_scaffold_XAEC_2083002 XAEC_scaffold_XAEC_2084675 XAEC_scaffold_XAEC_2017242 XAEC_scaffold_XAEC_2081946 XAEC_scaffold_XAEC_2085817 XAEC_scaffold_XAEC_2014436 XAEC_scaffold_XAEC_2084541 XAEC_scaffold_XAEC_2084544 XAEC_scaffold_XAEC_2081519 XAEC_scaffold_XAEC_2085400 XAEC_scaffold_XAEC_2083780 XAEC_scaffold_XAEC_2085975 XAEC_scaffold_XAEC_2085788 XAEC_scaffold_XAEC_2080465 XAEC_scaffold_XAEC_2081799 XAEC_scaffold_XAEC_2082353 XAEC_scaffold_XAEC_2084955 XAEC_scaffold_XAEC_2083584 XAEC_scaffold_XAEC_2009457 XAEC_scaffold_XAEC_2015036 XAEC_scaffold_XAEC_2010142 XAEC_scaffold_XAEC_2084387 XAEC_scaffold_XAEC_2082990 XAEC_scaffold_XAEC_2000468 XAEC_scaffold_XAEC_2084829 XAEC_scaffold_XAEC_2017859 XAEC_scaffold_XAEC_2085828 XAEC_scaffold_XAEC_2084550 XAEC_scaffold_XAEC_2080609 XAEC_scaffold_XAEC_2006542 XAEC_scaffold_XAEC_2007551 XAEC_scaffold_XAEC_2084324 XAEC_scaffold_XAEC_2017292 XAEC_scaffold_XAEC_2017698 XAEC_scaffold_XAEC_2085134 XAEC_scaffold_XAEC_2085135 XAEC_scaffold_XAEC_2084528 XAEC_scaffold_XAEC_2085304 XAEC_scaffold_XAEC_2017586 XAEC_scaffold_XAEC_2086103 XAEC_scaffold_XAEC_2082201 XAEC_scaffold_XAEC_2081679 XAEC_scaffold_XAEC_2014240 XAEC_scaffold_XAEC_2014818 XAEC_scaffold_XAEC_2081723 XAEC_scaffold_XAEC_2016802 XAEC_scaffold_XAEC_2007405 XAEC_scaffold_XAEC_2084246 XAEC_scaffold_XAEC_2082742 XAEC_scaffold_XAEC_2085436 XAEC_scaffold_XAEC_2081748 XAEC_scaffold_XAEC_2085432 XAEC_scaffold_XAEC_2083404 XAEC_scaffold_XAEC_2084005 XAEC_scaffold_XAEC_2015015 XAEC_scaffold_XAEC_2011594 XAEC_scaffold_XAEC_2083748 XAEC_scaffold_XAEC_2082380 XAEC_scaffold_XAEC_2083626 XAEC_scaffold_XAEC_2086049 XAEC_scaffold_XAEC_2016380 XAEC_scaffold_XAEC_2007273 XAEC_scaffold_XAEC_2085656 XAEC_scaffold_XAEC_2009564
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  • The Bulletin, 2020 Summer-Fall Issue

    The Bulletin, 2020 Summer-Fall Issue

    Vol. XXXVI No. 2 SUMMER-FALL 2020 the bulletinof the National Tropical Botanical Garden THE BULLETIN OF NTBG | SUMMER-FALL 2020 1 contents 3 MESSAGE FROM THE CEO/DIRECTOR ON THE COVER Planting hope The endemic Dubautia-Sadleria shrubland- fernland below the summit of Kawaikini, Kaua‘i’s highest peak (5,243 ft.), is a prime features example of healthy native Hawaiian habitat free of disease and invasive species. Photo for the future… by Ken Wood 6 HOW NTBG CONTRIBUTES TO PLANT HEALTH The Bulletin is a publication for supporters by NTBG staff of the National Tropical Botanical Garden, a not-for-profit institution dedicated to tropical plant conservation, scientific During this unprecedented pause, scientists, policy makers, and 14 TWO DECADES AFTER RESTORATION, research, and education. global leaders are highlighting the opportunity to safeguard our REFLECTIONS ON PI‘ILANIHALE environment in new ways as the world reopens. In the midst of We encourage you to share this HEIAU RISING publication with your family and friends. uncertainty, our core mission of saving plants is relevant, timely, by Chipper Wichman with Mike Opgenorth If your household is receiving more than and vital to a brighter future. Please consider joining us at this one copy and you wish to receive only critical juncture by using the enclosed envelope to make your one, please inform our Development 22 NTBG AUDITS THE SEED BANK Office at our national headquarters at: contribution today. To donate online, go to ntbg.org/donate. TAKING STOCK [email protected]. by Kelli Jones National Tropical Botanical Garden 3530 Papalina Road, Kalāheo 24 GOING NATIVE IN SOUTH FLORIDA Hawai‘i 96741 USA by Craig Morell Tel.
  • 9:00 Am PLACE

    9:00 Am PLACE

    CARTY S. CHANG INTERIM CHAIRPERSON DAVID Y. IGE BOARD OF LAND AND NATURAL RESOURCES GOVERNOR OF HAWAII COMMISSION ON WATER RESOURCE MANAGEMENT KEKOA KALUHIWA FIRST DEPUTY W. ROY HARDY ACTING DEPUTY DIRECTOR – WATER AQUATIC RESOURCES BOATING AND OCEAN RECREATION BUREAU OF CONVEYANCES COMMISSION ON WATER RESOURCE MANAGEMENT STATE OF HAWAII CONSERVATION AND COASTAL LANDS CONSERVATION AND RESOURCES ENFORCEMENT DEPARTMENT OF LAND AND NATURAL RESOURCES ENGINEERING FORESTRY AND WILDLIFE HISTORIC PRESERVATION POST OFFICE BOX 621 KAHOOLAWE ISLAND RESERVE COMMISSION LAND HONOLULU, HAWAII 96809 STATE PARKS NATURAL AREA RESERVES SYSTEM COMMISSION MEETING DATE: April 27, 2015 TIME: 9:00 a.m. PLACE: Department of Land and Natural Resources Boardroom, Kalanimoku Building, 1151 Punchbowl Street, Room 132, Honolulu. AGENDA ITEM 1. Call to order, introductions, move-ups. ITEM 2. Approval of the Minutes of the June 9, 2014 N atural Area Reserves System Commission Meeting. ITEM 3. Natural Area Partnership Program (NAPP). ITEM 3.a. Recommendation to the Board of Land and Natural Resources approval for authorization of funding for The Nature Conservancy of Hawaii for $663,600 during FY 16-21 for continued enrollment in the natural area partnership program and acceptance and approval of the Kapunakea Preserve Long Range Management Plan, TMK 4-4-7:01, 4-4-7:03, Lahaina, Maui. ITEM 3.b. Recommendation to the Board of Land and Natural Resources approval for authorization of funding for The Nature Conservancy of Hawaii for $470,802 during FY 16-21 for continued enrollment in the natural area partnership program and acceptance and approval of the Pelekunu Long Range Management Plan, TMK 5-4- 3:32, 5-9-6:11, Molokai.
  • The Bulletin, 2019 Fall/Winter Issue

    The Bulletin, 2019 Fall/Winter Issue

    Vol XXXV No. 3 FALL-WINTER 2019 the bulletinof the National Tropical Botanical Garden contents From all of us at NTBG, 3 MESSAGE FROM THE CEO/DIRECTOR ON THE COVER The green, red, and white in this American here's wishing you and your family the very best holly (Ilex opaca) illustration captures features the spirit of the season. This issue of The Bulletin features works by members of holiday season and a happy new year. 4 KEEP COOL, STAY DRY, AND YOU MAY NTBG’s Florilegium Society including LIVE LONG botanical artist and instructor Wendy Hollender (cover image). by Dustin Wolkis and Marian Chau The Bulletin is a publication for supporters of the National Tropical Botanical Garden, 8 SPLITTING HAIRS (EVEN WHEN THERE a not-for-profit institution dedicated to ARE NONE) tropical plant conservation, scientific by Dr. David H. Lorence research, and education. We encourage you to share this 18 INTERVIEW: DR. NINA RØNSTED, publication with your family and friends. NTBG’S NEW DIRECTOR OF SCIENCE If your household is receiving more than one copy and you wish to receive only AND CONSERVATION one, please inform our Development Office at our national headquarters at: [email protected]. in every issue National Tropical Botanical Garden 3530 Papalina Road, Kalāheo RED LISTED Hawai‘i 96741 USA 12 Tel. (808) 332-7324 Fax (808) 332-9765 THE GREEN THUMB [email protected] 13 www. ntbg.org 16 GARDEN SPROUTS ©2019 National Tropical Botanical Garden News from around the Garden ISSN 1057-3968 All rights reserved. Photographs are the property of NTBG unless otherwise noted.