Targeted gene sequencing and selection detection in ex situ collections of Brighamia insignis Christina Shehata1, Lauren Audi1,2, Hilary Noble2, Colby Witherup1,2, Jeremie Fant2, Norm Wickett2 1Northwestern University, Evanston, IL 60201; 2Chicago Botanic Garden, Glencoe, IL 60022 Introduction Results B_insignis_107_BI−001 Discussion B_insignis_UNK_BI−005 B_insignis_1_BI−006 B_insignis_132_BI−007 Brighamia insignis (family: Campanulaceae, subfamily: Lobelioideae) is a B_insignis_255_BI−014 B_insignis_255_BI−015 B_insignis_255_BI−020 While 206 genes were recovered during assembly, some genes were B_insignis_255_BI−031 B_insignis_255_BI−032 B_insignis_UNK_BI−034 B_insignis_565_BI−052 B_insignis_92_BI−073 species of caudiciform succulent endemic to Hawaii. Brighamia insignis is B_insignis_256_BI−077 B_insignis_wild_BI−083 B_insignis_wild_BI−084 not captured by the Campanulaceae probes in any of 83 samples (Figure B_insignis_wild_BI−087 B_insignis_wild_BI−095 B_insignis_wild_BI−097 B_insignis_UNK_BI−099 B_insignis_wild_BI−104 extinct in the wild, due to habitat loss, small population sizes, and the loss B_insignis_257_BI−107 B_insignis_1002_BI−109 B_insignis_106_BI−110 2). The probes were designed from Campanula erinus, a relatively distant B_insignis_UNK_BI−112 B_insignis_wild_BI−113 B_insignis_UNK_BI−124 1 B_insignis_UNK_BI−129 B_insignis_UNK_BI−131 of its native pollinator, Tinostoma smaragditis . Fortunately, due to their B_insignis_UNK_BI−1367 B_insignis_UNK_BI−139 B_insignis_MULT21_BI−153 species from Brighamia insignis, possibly explaining the lower success of B_insignis_UNK_BI−160 B_insignis_UNK_BI−201 B_insignis_UNK_BI−208 B_insignis_UNK_BI−211 B_insignis_UNK_BI−212 novel morphology, Brighamia insignis have been cultivated as ornamental B_insignis_UNK_BI−215 B_insignis_UNK_BI−218 B_insignis_UNK_BI−231 target capture for some particular probes. The overall diversity among B_insignis_UNK_BI−237 B_insignis_UNK_BI−251 B_insignis_wild_BI−252 B_insignis_wild_BI−253 B_rockii_wild_BR−008 plants and still exist in ex situ collections around the world. B_rockii_wild_BR−016 B_rockii_wild_BR−020 B_rockii_97_BR−077 B_rockii_wild_BR−098 the samples of Brighamia insignis was greater than expected, with B_rockii_wild_BR−099 B_rockii_UNK_BR−100 B_rockii_UNK_BR−101 B_rockii_UNK_HKM002 Genomic data obtained via next-generation B_insignis_UNK_HKM003 D_rhytidosperma_UNK_HKM004 B_rockii_wild_HW012 Cl_fauriei_wild_HW016 multiple points of divergence observed on the population tree along the Cl_fauriei_wild_HW025 T_kauaiensiswild_HW027 T_kauaiensiswild_HW031 D_rhytidosperma_UNK_HW040 sequencing methods provide valuable information D_rhytidosperma_UNK_HW041 D_kauensis_wild_HW042 D_kauensis_wild_HW043 Cy_pseudofauriei_wild_HW044 branch leading to the populations of Brighamia insignis (Figure 2). Cy_pseudofauriei_wild_HW046 Cy_leptostegia_wild_HW048 Cy_leptostegia_wild_HW049 Cy_leptostegia_wild_HW050 about putatively expressed regions of the genome. Cy_hardyi_wild_HW051 Cy_fissa_wild_HW052 Cy_fissa_wild_HW053 Cy_fissa_wild_HW054 93 genes were found to have an LSDnorm value greater than 0 (Figure Cy_fissa_wild_HW055 Cl_fauriei_wild_HW059 Cy_hardyi_wild_HW061 B_rockii_wild_HW063 B_rockii_wild_HW064 Identifying those genes under natural selection can D_waianaeensis_UNK_LY002 B_rockii_UNK_ML002 B_rockii_UNK_ML005 3), suggesting that these genes are adaptive candidates within the D_kauensis_UNK_NT005 B_insignis_UNK_QKP005 help conservation scientists understand the sampled populations of Brighamia insignis. While traditional population scaffold_XAEC_2031402 scaffold_XAEC_2009701 scaffold_XAEC_2085436 scaffold_XAEC_2084760 scaffold_XAEC_2014344 scaffold_XAEC_2082868 scaffold_XAEC_2083385 scaffold_XAEC_2085304 scaffold_XAEC_2014228 scaffold_XAEC_2012381 scaffold_XAEC_2013546 scaffold_XAEC_2010914 scaffold_XAEC_2085985 scaffold_XAEC_2072819 scaffold_XAEC_2005335 scaffold_XAEC_2084276 scaffold_XAEC_2085700 scaffold_XAEC_2082107 scaffold_XAEC_2081424 scaffold_XAEC_2085904 scaffold_XAEC_2006755 scaffold_XAEC_2017460 scaffold_XAEC_2000424 scaffold_XAEC_2086239 scaffold_XAEC_2082050 scaffold_XAEC_2085725 scaffold_XAEC_2009935 scaffold_XAEC_2011064 scaffold_XAEC_2001544 scaffold_XAEC_2085482 scaffold_XAEC_2079785 scaffold_XAEC_2016261 scaffold_XAEC_2081872 scaffold_XAEC_2080877 scaffold_XAEC_2011700 scaffold_XAEC_2014153 scaffold_XAEC_2083592 scaffold_XAEC_2014329 scaffold_XAEC_2006092 scaffold_XAEC_2080494 scaffold_XAEC_2085496 scaffold_XAEC_2078669 scaffold_XAEC_2001114 scaffold_XAEC_2071303 scaffold_XAEC_2086058 scaffold_XAEC_2070149 scaffold_XAEC_2003396 scaffold_XAEC_2079367 scaffold_XAEC_2005232 scaffold_XAEC_2082937 scaffold_XAEC_2015246 scaffold_XAEC_2082812 scaffold_XAEC_2081103 scaffold_XAEC_2017079 scaffold_XAEC_2005196 scaffold_XAEC_2017875 scaffold_XAEC_2013884 scaffold_XAEC_2017561 scaffold_XAEC_2001477 scaffold_XAEC_2080606 scaffold_XAEC_2082141 scaffold_XAEC_2009747 scaffold_XAEC_2014175 scaffold_XAEC_2009451 scaffold_XAEC_2081270 scaffold_XAEC_2084298 scaffold_XAEC_2082941 scaffold_XAEC_2064325 scaffold_XAEC_2080433 scaffold_XAEC_2013530 scaffold_XAEC_2004450 scaffold_XAEC_2083526 scaffold_XAEC_2015208 scaffold_XAEC_2006174 scaffold_XAEC_2057767 scaffold_XAEC_2017553 scaffold_XAEC_2001888 scaffold_XAEC_2001550 scaffold_XAEC_2000846 scaffold_XAEC_2011036 scaffold_XAEC_2037036 scaffold_XAEC_2083253 scaffold_XAEC_2017893 scaffold_XAEC_2077649 scaffold_XAEC_2084810 scaffold_XAEC_2006412 scaffold_XAEC_2084741 scaffold_XAEC_2009073 scaffold_XAEC_2017521 scaffold_XAEC_2084670 scaffold_XAEC_2006695 scaffold_XAEC_2085611 scaffold_XAEC_2086014 scaffold_XAEC_2079951 scaffold_XAEC_2083474 scaffold_XAEC_2081492 scaffold_XAEC_2079547 scaffold_XAEC_2017491 scaffold_XAEC_2083079 scaffold_XAEC_2017499 scaffold_XAEC_2017095 scaffold_XAEC_2084829 scaffold_XAEC_2085928 scaffold_XAEC_2083788 scaffold_XAEC_2012087 scaffold_XAEC_2030622 scaffold_XAEC_2011401 scaffold_XAEC_2082878 scaffold_XAEC_2083607 scaffold_XAEC_2012769 scaffold_XAEC_2083953 scaffold_XAEC_2085250 scaffold_XAEC_2016681 scaffold_XAEC_2079242 scaffold_XAEC_2084852 scaffold_XAEC_2085290 scaffold_XAEC_2084227 scaffold_XAEC_2014903 scaffold_XAEC_2009007 scaffold_XAEC_2083270 scaffold_XAEC_2074174 variation that influences the fitness of individuals. 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