SPME for GC Analysis

Getting Started with Solid Phase Microextraction

The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. Our experts can help you utilize SPME technology to maximize the benefits for virtually any application.

SPME is an innovative, solvent free technology that is fast, economical, and versatile.

SPME is a fiber coated with a liquid (polymer), a solid (sorbent), or a combination of both. The fiber coating removes the compounds from your sample by absorption in the case of liquid coatings or adsorption the case of solid coatings. Supelco services include... • Custom Capabilities SPME has gained widespread If you have special needs beyond what is listed, please inquire about a custom order. We can acceptance as the technique of preference honor many special requests for custom gauges, for many applications. coatings, field samplers, etc. • Available When You Need It • Easy Ordering • On-Time Delivery • Superior Support

TechTip Be sure to avoid overtightening GC injection port septa to avoid breaking or stripping the SPME fiber. We recommend the use of... pre-drilled septa or a septumless injector system.

SUPELCO 349

Getting Started in SPME...... 350 Introduction to SPME ...... 350 SPME Products and Accessories ...... 352 Fiber Assemblies andHolders ...... 352 SPME Samplers...... 354 SPME/HPLC Interface...... 355 SPME/HPLC Fibers andAccessories ...... 355 SPME/GC Inlet Liners...... 356 SPME Accessories...... 357 SPME Applications ...... 360 Amines...... 360 Aromatics...... 360 Explosives...... 360 Drugs ...... 361 Foods, Flavors andFragrances ...... 362 Hydrocarbons ...... 365 Pesticides...... 365 Herbicides...... 366 PCBs ...... 366 Phenols...... 366 Polymers and Oligomers ...... 367 Polynuclear Aromatic Hydrocarbons ...... 367 Semivolatiles...... 368 Solvents ...... 368 Odors ...... 369 Sulfur Compounds ...... 369 Volatiles...... 369

SUPELCO SUPELCO Solid Phase Microextraction 350

Order: 1.800.325.3010 Technical Service: 1.800.359.3041 Web: www.sigma-aldrich.com/supelco HPLC Analysis GC Analysis oi hs iretato:ASimpleSampleExtractionProcess Solid PhaseMicroextraction: Introduction toSPME Getting StartedinSPME Sample Extraction SPME offerssomeimportantadvantages: compounds, inbothliquidandgaseoussamples,foranalysisbyGC,GC/MS,orHPLC. SPME requiresnosolventsorcomplicatedapparatus.Itcanconcentratevolatileandnonvolatile The SPMEoperatingstepsaresimple: is attachedtoastainlesssteelplungersheathedbyprotectiveneedle. cases mixedwithasolidadsorbent(e.g.,divinylbenzenepolymerorporouscarbon).Thefiber An SPMEunitconsistsofalengthfusedsilicafibercoatedwithpolymermaterial,insome technique, SPME drawbacks, includinghighcostandexcessivepreparationtime.Auniquesample or supercriticalfluidextractionforsemivolatilesandnonvolatiles.Thesemethodshavevarious headspace methodsforconcentratingvolatiles;andliquid-liquidextraction,solidphase The extractionoforganiccompoundsfromasamplematrixusuallyconsistspurge-and-trapor 1 US patentno.5,691,206.European #0523092.Technology licensedexclusivelytoSupelco. ● ● ● ● ● ● ● ● ● ● ● ● ● ● ● sample Pierce Expose thefiberandclose sealing clamp. Insert theneedleintoSPME/HPLCinterfacedesorptionchamber (injectionvalveinloadposition). Retract thefiberandremoveneedle. Depress theplunger,exposingfiberinheatedzoneof injectortodesorbtheanalytesontocolumn. Insert theneedleintoGCinjectorport. Retract thefiberintoneedleandremovefrom samplevial. Analytes adsorbtothefiberin230minutes. Depress theplungertoexposefiberliquidsampleorheadspaceabovesample. With thefiberretracted,passneedlethroughsamplevialseptum. Versatile –adaptstoanyGCorHPLCsystem,canbeautomatedwithVarianautosamplers Economical andreusable–morethan50extractionsperfiberonaverage Solvent reduction–minimizestheuseofsolvents,andtheirdisposal Fast –reducessamplepreparationtimeby70% Switch theinjectionvalveto“load,” retractthefiber,andremoveneedle. Switch theinjectionvalveto“inject.” Mobilephasewillflowthroughthechamber,desorbanalytes andcarrythemtothec Expose fiber/ 1 , eliminatesmostdrawbackstoextractingorganics. Retract HPLC GC Insert fiber GC inlet Pierce into Desorb mobile Expose fiber/ fiber/ add To HPLC column Mobile Retract Retract fiber/ 796-0467 olumn.

P000106 Some typicalapplicationsforSPMEare: You Want to Extract Choose aFiberAccordingtotheAnalytes weights andpolarityoftheanalytes. Determine thetypeoffiberyouneedaccordingtomolecular HPLC interface. injection portofaGCorinthedesorptionchamberanSPME/ Analytes aredesorbedfromthefiberbyexposingin headspace extraction,usingapolymer-coatedfusedsilicafiber. immersion orheadspaceextraction,asolidsample,by In SPME,youcanadsorbanalytesfromaliquidsample,by ● ● ● ● ● ● ● ● ● ● ● ● ● ● ppt odoranalyses or drugsinurine/serum Toxicology analyses:bloodalcohol samples Forensic analysesofarson/explosives Flavor analysesoffoodproducts Environmental analysesofwatersamples polymers andsolidsamples Headspace analysisoftraceimpuritiesin Surfactants, otherindustrialapplications divinylbenzene/Carboxen onPDMSfiber. For anexpandedrangeofanalytes(C3-C20),usea50/30 Carboxen fiber. For trace-levelvolatilesanalysis,usea75µmPDMS/ HPLC. A 60µmPDMS/DVBfiberisageneralpurposefor fiber. polydimethylsiloxane/divinylbenzene (PDMS/DVB)-coated adsorbed moreefficientlyandreleasedfasterwitha65µm More volatilepolaranalytes,suchasalcoholsoramines,are 85µm polyacrylate-coatedfiber. To extractverypolaranalytesfromsamples,usean PDMS fiber. effectively extractedwitha30µmPDMSfiberor7µm Larger molecularweightorsemivolatilecompoundsaremore 100µm polydimethylsiloxane(PDMS)-coatedfiber. Low molecularweightorvolatilecompoundsusuallyrequirea the field,thenstorethemfortransporttolaboratory. sampler, allowsyoutoconcentrateorganicsfromairorwater,in A specializedtypeofmanualSPMEholder,theportable HPLC interface. The automatedholderalsoisrequiredforusewithanSPME/ sample extraction. The autosamplercontrolsfibermovement,allowingautomatic The automatedholderissimilarindesigntothemanualversion. during extractionordesorption. locking mechanismsecuresthefiberinexposedposition port. Aspringretractsthefiberintoprotectiveneedleanda the depthoffiberintroductionintosamplevialorinjection The manualholderhasanadjustableneedlegaugethatcontrols interface. Bothversionsincludethefollowingfeatures: use andoneforwithautosamplersorourSPME/HPLC SPME fiberholdersareavailableintwoversions,oneformanual hexane. 100µm, 30µm,and7µmPDMS-coatedfiberscannotbeusedwith Most ofthesefibersarecompatiblewithHPLCsolvents,butthe ● ● hub. A windowinthebarrel,toidentifyfiberbyitscolor-coded A handtightneedlehubassemblyforquickinterchangeoffibers. Getting StartedinSPME

Introduction toSPME P000144

Order: 1.800.325.3010 Technical Service: 1.800.359.3041 Web: www.sigma-aldrich.com/supelco 351 SUPELCO Solid Phase Microextraction SUPELCO Solid Phase Microextraction 352 ECITO AG A.N.PIECT O PRICE CAT. NO. PRICE CAT. NO. GAUGE DESCRIPTION 65µm polydimethylsiloxane/divinylbenzeneonaStableFlexfiber 85µm Carboxen/polydimethylsiloxaneonaStableFlexfiber 70µm Carbowax/divinylbenzeneonaStableFlexfiber 70µm Carbowax/divinylbenzeneonaStableFlexfiber 65µm polydimethylsiloxane/divinylbenzene 100µm polydimethylsiloxane 75µm Carboxen/polydimethylsiloxane Order: 1.800.325.3010 Technical Service: 1.800.359.3041 Web: www.sigma-aldrich.com/supelco SPME FiberAssemblies(pk.of3) SPME FiberAssemblies Fiber AssembliesandHolders SPME ProductsandAccessories 50µm Carbowax/templatedresin FOR SURFACTANTS ANDOTHERPOLARANALYTES (HPLCUSEONLY) 60µm polydimethylsiloxane/divinylbenzene FOR AMINESANDPOLARCOMPOUNDS(HPLCUSEONLY) 50/30µm divinylbenzene/Carboxenonpolydimethylsiloxanea FOR TRACELEVEL(MW40-275) StableFlexfiber 50/30µm divinylbenzene/Carboxenonpolydimethylsiloxanea FOR FLAVORS (VOLATILES ANDSEMIVOLATILES, C3-C20)(MW40-275) 65µm Carbowax/divinylbenzene FOR ALCOHOLSANDPOLARCOMPOUNDS(MW40-275) 30µm polydimethylsiloxane FOR NONPOLARSEMIVOLATILES (MW80-500) 7µm polydimethylsiloxane FOR NONPOLARHIGHMOLECULARWEIGHTCOMPOUNDS(MW125-600) 85µm polyacrylate FOR POLARSEMIVOLATILES (MW80-300) 65µm polydimethylsiloxane/divinylbenzene FOR VOLATILES, AMINES,ANDNITROAROMATIC COMPOUNDS(MW50-300) 100µm polydimethylsiloxane FOR VOLATILES (MW60-275) 75µm Carboxen/polydimethylsiloxane FOR GASESANDLOWMOLECULARWEIGHTCOMPOUNDS(MW30-225) Fibers are1cmlongunlessnotedotherwise. users mustorderbothaholderandfiberassembly. the typeofcoatingonfiber.Chooseassemblythatisappropriateforholder:manualorautosampler/HPLC.Firsttim reuse, simplyconditionwithsolventorheatbeforeandaftereveryanalysis.Eachassemblyhasacolor-codednotchedhubin SPME fiberassembliescanbereusedforupto100analyses,ormore,dependingontheapplicationandcaretheyaregiven. 2cm StableFlexfiber 6 5 4 3 2 1 High retentionfortraceanalysis. compared tothesamecoatingonastandardfusedsilicacore.2cmfiberassemblycontains nospring. Coating bondedtoaflexiblefusedsilicacore,yieldingmorestablecoatingonless breakablefiber. Theremaybeasligh Designed forusewithMerlinMicrosealsealingsystem;alsocanbeusedother septumless systems. Partially crosslinkedphase. Bonded phase. Nonbonded phase. 5 3 5 2 1 1 1 3 3 3 3 3 3 3 5 5 6 5 IE IE ODR530UFIBERHOLDER57331OR57347-U FIBERHOLDER57330-U FIBER 4534 70 57305 57327-U 57311 57304 57301 57326-U 24 57319 57310-U 57335-U 24 23 24 57300-U 23 57334-U 24 57318 24 23 24 4——535 57315 — 57317 — — — — — 57329-U 24 57348-U 24 57313 57337-U 24 57309 57328-U 57303 24 57336-U 57312 23 57308 24 24 57302 24 24 4 4 4 4 74- 74- 57345-U 57341-U 57346-U 57343-U 57342-U 57344-U 73- 73- 57339-U 57338-U AUL SDWT AUTOMATIC/HPLC,WITH USED MANUAL, USEDWITH t differenceinextractionselectivity dicating e SPME For i o PEHL nlss—— PRICE — CAT. NO. PRICE CAT. NO. Odors Flavorsand Kit 4–For 100µm polydimethylsiloxanecoating 50µm Carbowax/templatedresincoating 60µm polydimethylsiloxane/divinylbenzenecoating Kit 3–ForSPME/HPLCAnalysis 65µm Carbowax/divinylbenzenecoating 65µm polydimethylsiloxane/divinylbenzenecoating 75µm Carboxen/polydimethylsiloxanecoating Water PolarOrganicsin Kit 2–ForVolatile or 7µm polydimethylsiloxanecoating 100µm polydimethylsiloxanecoating coating 85µm polyacrylate Semivolatiles and Kit 1–ForVolatiles 70µm Carbowax/DVBcoating 85µm Carboxen/PDMScoating 50/30µm DVB/Carboxen/PDMScoating 65µm PDMS/DVBcoating SPME StableFlexFiber Assortment Kit DESCRIPTION 100µm polydimethylsiloxanecoating PEFbrAsrmn is – 24-gauge fibers,1fiberofeachtypeaslisted. SPME FiberAssortmentKits web site: appear beforetheApplications section. ForalistofSPMEjournalarticles,contactourTechnical Servicechemists,orvisito Applications involvingSPMEare includedintheApplicationssectionatendofthischapter. TitlesofourSPMEpublication RELATED INFORMATION 75µm Carboxen/polydimethylsiloxanecoating 65µm polydimethylsiloxane/divinylbenzenecoating www.sigma-aldrich.com/supelco Fiber AssemblyUsedWith SPMEHolders57331and57347-U Fiber AssemblyUsedWith SPMEHolder57330-U(ForManualUse) (standard 24gaugeneedle) P000812 P000813 P000815 Plain . Notched Hub Plain Hub 7960602 SPME ProductsandAccessories 72- 72- 57321-U 57320-U FIBER HOLDER 57330-U HOLDER 57331 or 57347-U 57331 or HOLDER FIBER HOLDER57330-U 72- 72- 57325-U 57324-U 57551-U 57550-U 70 537 57307 57306 SDWT USEDWITHFIBER USED WITH (23 gaugeneedle) Fiber AssembliesandHolders Notched P000816 72- 57323-U ur s

Order: 1.800.325.3010 Technical Service: 1.800.359.3041 Web: www.sigma-aldrich.com/supelco 353 SUPELCO Solid Phase Microextraction SUPELCO Solid Phase Microextraction 354

Order: 1.800.325.3010 Technical Service: 1.800.359.3041 Web: www.sigma-aldrich.com/supelco SPME PortableFieldSampler Fiber HolderforCTCCombiPAL Autosampler* Fiber HolderforAutomatedSamplingorHPLCAnalysis Fiber HolderforManualSampling SPME FiberHolders SPME Samplers SPME ProductsandAccessories 0mCroa/V 74- 57340-U 504823 504858 20638 57347-U SPME 504831 Septum Removing Tool Replacement Septa (pk. of 100) 57330-U 70µm Carbowax/DVB 65µm PDMS/DVBStableFlexFiber 100µm PDMS Fiber 75µm PDMS/Carboxen Fiber SPME PortableFieldSamplers(pk.of2) For CTC Autosampler 57331 For Varian Autosampler or HPLC Analysis For Manual Sampling Fiber Holder ECITO A.N.PRICE CAT. NO. DESCRIPTION * AutosamplerdistributedbyVarian,Leap,andGerstel port duringfiberdesorption. needle insertionintoasamplecontainer,ortheinjection Five slotsintheneedleguide/depthgaugecontroldepthof semivolatiles. larger volatiles,andaCarbowax/DVBfiberforpolar purpose PDMSfiber,aPDMS/DVBfiberforsemi-volatilesand (PDMS)/Carboxen fiberfortracelevelsofvolatiles,ageneral tory air.Fourfibersareavailable:apolydimethylsiloxane monitor typicalHPLCandGCsolventsatppblevelsinlabora- compounds inair.Inourstudies,thesamplerallowedusto The portablefieldsampleralsoefficientlydetectsorganic and isdisposedofwhenthefibernolongerusable. whole watersamples.Thesamplercanbereused50-100times, field samplerweresignificantlylowerthanlossesfromstored storage lossesforpesticidesextractedandstoredusingaportable are safelysealedbehindareplaceableseptum.Table1showsthat semivolatile compoundsfromfieldsamples.Extracted economical wayofextractingandtransportingvolatile -TheSPME portablefieldsamplerisanefficientand Air Concentrate andStoreAnalytesfromWater;SampleIndoor automated sampling. Use thisholderwithSPMEfiberassembliesthataredesignedfor requirements. Varian InstrumentDivisionforinformationconcerningsystem necessary foroperationwiththeVarianAutoSampler–contact with ourSPME/HPLCinterface.AnSPMEupgradekitis Use thisfiberholderwithaVarian8100/8200AutoSampleror The fibercanbelockedintheexposedposition. correct placementintheheatedzoneofGCinjectionport. An adjustabledepthguidepositionsthefiberforsamplingand First timeusersmustorderbothaholderandfiberassembly. reusable indefinitelyandacceptsthereplaceablefiberassembly. fiber duringanalyteadsorptionanddesorption.Theholderis The holderprotectsthecoatedfiber,andcontrolsexposureof 75- 57359-U NLT FIBER ANALYTE idn 2-4TP 8-54 -68 -8 -3 -53 -10 -84 -83 -81 -3 -68 Thionazin -15 TEPP +4 -88 -7 -74 -74 Simazine -14 Methylparathion -6 -2 -60 Parathion Phorate -98 Sulfotep Methoxychlor -3 -93 -82 -12 -83 -57 Malathion -8 Lindane -10 -12 -15 Heptachlor epoxide Famphur Endrin ketone Disulfoton DDE Atrazine for fiberreplacement Fiber holderdisassembles Samples Field SamplerisMuchHigherthanforStoredWater Table ofPesticidesExtracted/StoredinSPME 1.Recovery 3 2 1 SPME. Water samplestoredinasilanizedvial(24hours/4°C),thenextracted by Pesticides extractedbySPMEandstored on PDMSfiber(24hours/4°C). Relative toimmediateanalysis.10ppbeachpesticideinwater. % LOSSONSTORAGE PESOE PESTORED SPME STORED SPME

2 G000351 WATER

1 Fiber holder for automated sampling/HPLC 57331 3 en-%-75% -8% Mean WATER FIBER ANALYTE % LOSSONSTORAGE Fiber holder for manual sampling 57330-U

Portable field sampler 504831

P000103 material isinjectedontothecolumn(staticdesorption). to thefiber,fibercanbesoakedinmobilephasebefore (dynamic desorption).Whenanalytesaremorestronglyadsorbed Analytes oftencanberemovedviaastreamofmobilephase the SPMEfiberormobilephasearestainlesssteelVESPEL. the ferruleagainstSPMEneedle.Allsurfaceswhichcontact tight (to5000psi/35mPa)byclosingtheclampandcompressing the injectionvalvein“load”position.Theunitismadeleak- The SPMEfiberisintroducedintothedesorptionchamberwith VESPEL ferrule,andasealingclamp. stainless steelbodyandcompressioncap,adouble-tapered SPME/HPLC Interface, ReplacementParts,andAccessories SPME/HPLC Interface, Easy HPLCAnalyseswithSPME for SPME/HPLC Interface 0µ oyiehlioae533U 57323-U 100µm polydimethylsiloxane 60µm polydimethylsiloxane/divinylbenzene, 50µm Carbowax/templatedresin, Fiber Kit3(onefiberofeach) 57301 100µm polydimethylsiloxane, for volatiles 57305 85µm polyacrylate, for polar semivolatiles 57351 50µm Carbowax/templatedresin, 57353 60µm polydimethylsiloxane/divinylbenzene, 55045 SPME holderforHPLCuse 58830-U Rhebuild kit for Rheodyne valve 7125 Rotor seal for Rheodyne valve 7125 Ferrules (pk. of 10) Rheodyne valve version (includes2ferrules) SPME/HPLC Interface Fiber assembliesforHPLCuse ECITO A.N.PRICE CAT. NO. DESCRIPTION chamber includesaPEEK HPLC system.Easilyinstalledandremoved,thedesorption and adesorptionchamberthatreplacestheinjectionloopin separation. Theinterfaceconsistsofasix-portinjectionvalve the adsorbedanalytes,anddeliverthemtocolumnfor interface allowsmobilephasetocontacttheSPMEfiber,remove advantage ofthetimeandcostsavingsofferedbySPME.The The SPME/HPLCinterfaceenablesHPLCanalyststotake and beverageapplications. biological fluids–andSPMEalsohaspharmacologicalfood be effectiveformonitoringdrugsanddrugmetabolitesin Investigators inseverallaboratorieshaveshownthatSPMEcan o ufcat 71 57315 57317 for surfactants general purpose for drugs,vitamins,preservatives, 1 indefinitely. First timeusersmustorderbothholderandfiberassembly. Holderisreusable (polyetheretherketone) 1 1 73 57331 needleguide,a SPME / HPLC Interface, FibersandAccessories SPME /HPLCInterface, SPME/HPLC Interface: Operation SPME/HPLC Interface:

following publications: For moreinformationaboutSPME/HPLCanalyses,requestthe RELATED INFORMATION G000352

372 SPMEforCarbamates SPMEforFoodAntioxidantsandPreservatives SPMEforSurfactants T397121 SPMEforPAHs T396110 SPMEforExplosives T396106 T396099 T396098 To Interface To Static Desorption(noflow) o Title No. To HPLC Column SPME ProductsandAccessories Mobile Phase Valve Syringe Solvent from Pump from SPME FiberHolder Waste Needle Guide Compression Cap Septum-Piercing Double Tapered SPME Fiber

Desorption

Chamber From Interface From Solvent

Needle

Ferrule From Interface From To HPLC Column Sample Injection Valve Mobile Phase from Pump Waste

To Interface

Order: 1.800.325.3010 Technical Service: 1.800.359.3041 Web: www.sigma-aldrich.com/supelco 355 SUPELCO Solid Phase Microextraction SUPELCO Solid Phase Microextraction 356

Order: 1.800.325.3010 Technical Service: 1.800.359.3041 Web: www.sigma-aldrich.com/supelco SPME /GCInletLiners SPME ProductsandAccessories MAKE SPME/GCPEAKSSHARPER FIGURE A.NARROWBOREINLETLINERS a.N. 24154 Cat. No.: 57300-U Cat. No.: oun VOCOL,60mx0.25mmID1.5µm Column: are:helium, 40cm/sec Carrier: .Trichlorofluoromethane (50ppbeach) 5. Chloroethane 4. Bromomethane 3. Vinyl chloride 2. Chloromethane 1. vn 35°C Oven: ie:PDMS,100µm Fiber: 2 n. 230°C Inj.: 3456 P000137 2mm IDLiner(Standard) 1 1 2 Min 0.75mm IDLiner 2

26312,05 3 3 4 4 26329,01 26375,01 5 5

26358,01 G000353

26364,05

794-0051,0050 Inlet LinersforSPME Using SupelcoInletLiners Achieve SharperPeakswithSPME/GCAnalyses, ECITO A.N.PRICE CAT. NO. (5880, 5890SERIES,6890) AGILENT/HP DESCRIPTION inlet liners,request request For moreinformationaboutanalysis ofVOCsbySPME/GC, RELATED INFORMATION Flash On-Column,Varian SPMEInjector SPME INSERTS SHIMADZU GCMODELS17A INJECTOR) (SPL-17 INJECTOR) (SPL-14 SHIMADZU GCMODELS14/15A/16 (SPL-G9/15 Injector) SHIMADZU GCMODELS9A/15A/16 (Auto SystemSplit/SplitlessInjector) PERKIN-ELMER VARIAN 1093/1094SPIINJECTORS VARIAN 1078/1079SPLITLESS VARIAN 1075/1077INJECTORS by SPME,helpstoachieveexcellentchromatography. liner, combinedwithefficient,solvent-freesampleintroduction tion ofactivesamplecomponents.Usingtheappropriateinlet thoroughly deactivatesSupelcoinletlinerstominimizeadsorp- inert. Ourproprietary,high-temperaturesilanizationtechnique To minimizesamplelossorpeaktailing,theinletlinermustbe fiber (FigureA). demonstrate thatthecompoundsarerapidlydesorbedfrom The sharppeaksobtainedwiththe0.75mmIDlineralso rapidly introducesanalytesontothecolumninanarrowband. compared toaconventional,largervolume2mmIDliner,and SPME/GC, a0.75mmIDinletlinerincreaseslinearvelocity, introduction forspecificinjectiontechniques.Whenanalyzingby GC injectionportlinersaredesignedforoptimumsample k f52340 26364,05 pk. of 5 26329,25 26329,01 26329,05 26312,05 pk. of 25 pk. of 5 Each pk. of 5 k f2 67,5 26375,25 26375,01 26375,05 pk. of 25 pk. of 5 Each k f2 2392 26339,25 26339,01 26339,05 26335,25 26335,01 26335,05 pk. of 25 pk. of 5 Each pk. of 25 pk. of 5 Each 26364,25 26364,01 26364,05 26378,01 26378,05 26358,25 26358,01 26358,05 pk. of 25 pk. of 5 Each pk. of 5 Each pk. of 25 pk. of 5 Each Application NoteT394056. T196899. For moreinformationon Thermometer 40mL Vial Holder Heat/Stir Plate Manual SPMESamplingStand hroee,5 73 PRICE 57332 33313-U CAT. NO. 57358-U Thermometer, 5” Corning Z262129-1EA heat/stir plate, 120VAC 40mL Vial Holder Vial puck for 15mL vials SPME sampling stand 57357-U for 15mL vials 57333-U SPME sampling stand for 4mL vials DESCRIPTION sampling standandaheat/stirplate. For monitoringsampletemperaturewhenusingtheSPME SPME samplingofodorsorothervolatiles.Holdssix40mLvials. Use thisaluminumblockforheating/stirringduringheadspace range is40-550°C,stirring60-1200rpm. Fits compactlyonthebaseofSPMEsamplingstand.Heating 4mL unit.Notforusewithautomated/HPLCfiberholders. replacement forthe15mLunit,ortousevialswith vials. Orderthe15mLvialpuck(Cat.No. dates 4mLvialsonly;Cat.No. consistent fiberimmersiondepth.Cat.No. Holds eightvialswhilesupportingtheSPMEsyringefor SPME SamplingStand 57357-U Heat/Stir Plate accommodates 15mL 57358-U) as a as 57358-U) 57333-U

P000123 accommo- SPME InletGuide LB-2 SeptaforSPME Pre-Drilled Thermogreen Merlin MicrosealHighPressureSepta ECITO A.N.PRICE CAT. NO. 57356-U PRICE 23168 SPME inlet guide 23167 DESCRIPTION CAT. NO. 23162-U 23161 11mm (pk. of 50) 11mm (pk. of 25) 9.5mm (pk. of 50) 9.5mm (pk. of 25) DESCRIPTION ECITO A.N.PRICE 24818-U CAT. NO. 24816-U 24815-U 24814-U 1 replacement septum 1 Varian nut, 1 septum, & 24817-U 1 FOR VARIANGCMODELS3400,3800(1078,1079INJECTORS) inlet adapter 1 replacement septum 1 nut and 1 septum 1 nut and 2 septa FOR AGILENT/HPGCMODELS5800,5900SERIES,6890 DESCRIPTION chromatographs. Microseal sealingsystemandmostVarianHewlett-Packard thermal desorptionprocess.InterchangeableamongMerlin Secures theSPMEfiberholderininjectionportduring exclusive toSupelco. tures –from100°Cto350°C.Rubberformulation Extremely lowbleedoverawiderangeofinlettempera- peaks. Alreadyconditioned,ready-to-use. septum coringthatcancauseextraneous tolerance –idealforautosamplers.Reduce Easier needlepenetrationandhighpuncture at 2-100psi.Useonlywith23gaugeSPMEfiberassembly. conventional septa.Thenewhighpressureunitsallowoperation inlet system.Twosequentialsealsprovideamuchlongerlifethan standard septumandnutonacapillaryorpurgedpacked Merlin Microsealsystem,apatentedlong-lifereplacementforthe To eliminateseptumcoringduringSPMEinjections,usethe Eliminate siloxanebackground,prolongseptumlifetime. SPME ProductsandAccessories 57356-U

P000136 SPME InletGuide SPME Accessories

P000047 P000096

Order: 1.800.325.3010 Technical Service: 1.800.359.3041 Web: www.sigma-aldrich.com/supelco 357 SUPELCO Solid Phase Microextraction SUPELCO Solid Phase Microextraction 358

Order: 1.800.325.3010 Technical Service: 1.800.359.3041 Web: www.sigma-aldrich.com/supelco Vials forVarian 8200Autosampler SPME Accessories SPME ProductsandAccessories io et 00234 27364 1000 27351 100 27369-U 27120-U 1000 27032 27217 27356 27008 100 PRICE 27159 27031 27114 27111 27220-U 1000 100 100 100 100 100 1000 1000 100 CAT. NO. 100 Amber, preassembled, phenolic caps & PTFE/ silicone Clear, preassembled, phenolic caps & PTFE /silicone QTY. 15mL screwtopvials(21mmx70mm) WITH 15MLVIALHOLDERPUCK Viton Septa Viton Septa (11mm) White PTFE silicone septa White PTFE silicone septa (11mm) Phenolic cap with hole Open closuresandseptafor4mLvials Vials only, amber silanized 27006 Vials only, 27136 clear PRICE silanized Vials only, clear silanized Vials only, amber Vials only, amber 100 100 Vials only. clear CAT.Vials NO. only, clear Amber, preassembled, phenolic caps & PTFE/silicone Clear, 27297 preassembled, phenolic caps & 27296 PTFE/silicone QTY. 4mL screwtopvials(15mmx45mm) 27295 WITH 4MLVIALHOLDERPUCK 27294 DESCRIPTION 1000 100 27199 Vials forSPMESamplingStand 27198 1000 100 27300 *Autosampler distributedbyLeap,Varian andGerstel 100 Adjustable crimperfor20mmseals(5-100mL) PRICE 100 Closures (Tin 100 Plate, Magnetic Seals) with PTFE lined silicone VIALS AND20mL CLOSURES ANDACCESSORIESFORBOTH10mL 27532 27531 27539 20mL clear glass (22.6mm x 75mm) 20mL clear glass CAT.(22.6mm NO. x 75mm) 10mL clear glass (22.6mm 100 x 46mm) 100 10mL clear glass (22.6mm 100 x 46mm) 27245 ROUND BOTTOM QTY. 27286 33146-U 20mL 27385 clear glass (23mm x 75mm) 10mL clear glass (23mm x 46mm) 100 FLAT BOTTOM 36 144 DESCRIPTION 36 Headspace Vials forCTCAutosampler(CombiPAL)* 20mm PTFE/silicone septa (0.030”) 20mm seal with thin Viton septa (0.030”) 20mm seal with thin Viton septa (0.030”) CLOSURES FOR10MLVIALS Clear vials only, 10mL Crimped top Clear vials only, 10mL Crimped top 10ML SAMPLEHEADSPACE VIALS24.5MMX50MM(FORTHINSEALSDESIGNEDFORSPME) Amber, 2mL Clear, 2mL LARGE OPENING2MLVIALS(12MMODX32MM)WITHPOLYPROPYLENE CAPS,PTFE/SILICONESEPTA DESCRIPTION 21- 22316-U 1 71- 27115-U

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