IAPT Chromosome Data 30 TAXON 68 (5) • October 2019: 1124–1130

Marhold & Kucˇera (eds.) • IAPT chromosome data 30 TAXON 68 (5) • October 2019: 1124–1130

IAPT CHROMOSOME DATA

IAPT chromosome data 30 Karol Marhold (ed.),1,2 Jaromír Kucˇera (ed.),1 Erton Mendonça de Almeida,3 Lânia I.F. Alves,4 Claudia Araneda-Beltrán,5 Carlos M. Baeza,5 Evgeny V. Banaev,6 Fabiane R.C. Batista,4 N. Ivalú Cacho,7 Olga A. Chernyagina,8 Marely Cuba-Díaz,9 Andrey S. Erst,6,10 Leonardo P. Felix,11 Eliana Regina Forni Martins,12 Maria J. Gomes de Andrade,13 Polina D. Gudkova,10,14 Raghbir Chand Gupta,15 Kuljit Kaur,15 Mandeep Kaur Aulakh,15 Aleksandr A. Korobkov,16 Vera A. Kostikova,6 Violetta V. Kotseruba,16 Denis A. Krivenko,10,17,18 Igor V. Kuzmin,19 Zhi-Min Li,20 Samara S. Matos,13 Enoque Medeiros Neto,11 André L. Melo,21 Elizaveta Yu. Mitrenina,10 Viktor O. Nachychko,22 José Achilles L. Neves,11 José Rubens Pirani,23 Nina S. Probatova,24 María V. Romero-da Cruz,23 Eduardo Ruiz-Ponce,5 Manjit Inder Singh Saggoo,15 Fabiano J. Santos,25 Géssica S. Santos,13 Pollyana K. Silva,4 Vijay Singh,15 Pamela C.S.S. Souza,25 Tod Stuessy,26,27 Alexander P. Sukhorukov,28 Hang Sun,29 Wen-Guang Sun,29,30,31 Alexander N. Tashev,32 Mariya A. Tomoshevich,6 Oscar Toro-Núñez,5 Estrella Urtubey,33 Vera L.C. Vale,34 Mariya S. Voronkova,6 Wei Wang,35 Kunli Xiang35 & Daniela C. Zappi36 1 Plant Science and Biodiversity Centre, Institute of Botany, Slovak Academy of Sciences, Dubravska cesta 9, 845 23 Bratislava, Slovak Republic 2 Department of Botany, Charles University, Benatska 2, 128 01 Praha, Czech Republic 3 Laboratory of plant Cytogenetics and Evolution, Department of Botany, Federal University of Pernambuco, Recife, Pernambuco, Brazil 4 Laboratório de Citogenética Vegetal, Instituto Nacional do Semiárido - INSA, Campina Grande, Paraíba, Brazil 5 Departamento de Botánica, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile 6 Central Siberian Botanical Garden SB RAS, Zolotodolinskaya Str. 101, 630090 Novosibirsk, Russia 7 Instituto de Biología, Universidad Nacional Autónoma de México, CDMX 04510, México 8 Kamchatka Branch of the Pacific Geographical Institute, FEB RAS, 6 Partizanskaya Str., 683000, Petropavlovsk-Kamchatskii, Russia 9 Laboratorio de Biotecnología y Estudios Ambientales, Departamento de Ciencias y Tecnología Vegetal, Escuela de Ciencias y Tecnologías, Campus Los Ángeles, Universidad de Concepción, Chile 10 National Research Tomsk State University, Laboratory of Herbarium, Lenin Ave. 36, Tomsk 634050, Russia 11 Department of Biological Sciences, Federal University of Paraíba, Areia, Paraíba, Brazil 12 Instituto de Biologia, Universidade Estadual de Campinas – UNICAMP, Brasil 13 Programa de Pós-Graduação em Biodiversidade Vegetal, Universidade do Estado da Bahia, Departamento de Educação, Campus VIII, Rua da Gangorra, 503, CHESF 48608-240, Paulo Afonso, Bahia, Brazil 14 Department of Biology, Altai State University, Lenin Ave. 61, Barnaul 656049, Russia 15 Department of Botany, Punjabi University, Patiala, 147002, India 16 V.L. Komarov Botanical Institute of the Russian Academy of Sciences, Prof. Popov Str. 2, 197376 St. Petersburg, Russia 17 Siberian Institute of Plant Physiology & Biochemistry of the Siberian Branch of the Russian Academy of Sciences, Lermontov Str. 132, 664033 Irkutsk, Russia 18 Western Baikal Protected Areas, Baikalskaya Str. 291B, 664050 Irkutsk, Russia 19 Tyumen State University, Department of Botany, 10 Semakova str., Tyumen 625023, Russia 20 School of Life Science, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Yunnan Normal University, Kunming, China 21 Universidade Federal Rural de Pernambuco, Unidade Acadêmica de Serra Talhada, Avenida Gregório Ferraz Nogueira, S/N, José Tomé de Souza Ramos, 56909-535, Serra Talhada, Pernambuco, Brazil 22 Botanical Garden of Ivan Franko National University of Lviv, 44 Cheremshyna Str., Lviv 79014, Ukraine 23 Instituto de Biociências, Universidade de São Paulo - USP, Brasil 24 Federal Scientific Center of the East Asian Terrestrial Biodiversity, FEB RAS, 159 Stoletya Prospect, 690022, Vladivostok, Russia 25 Universidade do Estado da Bahia, Departamento de Educação, Campus VIII, Rua da Gangorra, 503, CHESF 48608-240, Paulo Afonso, Bahia, Brazil 26 Herbarium and Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, 1315 Kinnear Road, Columbus, Ohio 43212, U.S.A.

All materials for the chromosome column should be submitted electronically to: Karol Marhold, [email protected]. The full version of this contribution is available in the online edition of TAXON appended to this article. The following citation format is recommended: Dorogina, O.V., Emtseva, M.V., Agafonov, A.V. & Asbaganov, S.V. 2019. IAPT chromosome data 29/1. In: Marhold, K. & al., IAPT chromosome data 29. Taxon 68: 880, E1–E2. © 2020 International Association for Plant Taxonomy

1124 Version of Record TAXON 68 (5) • October 2019: 1124–1130 Marhold & Kucˇera (eds.) • IAPT chromosome data 30

27 Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, 1030 Vienna, Austria 28 Lomonosov Moscow State University, Department of Higher Plants, Biological Faculty, Moscow 119234, Russia 29 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China 30 School of Life Science, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Yunnan Normal University, Kunming, China 31 University of Chinese Academy of Sciences, Beijing, China 32 Department of Dendrology, University of Forestry, 1797 Sofia, Bulgaria 33 Instituto de Botánica Darwinion, Labardén 200, San Isidro, Buenos Aires, Argentina 34 Programa de Pós-Graduação em Biodiversidade Vegetal, Universidade do Estado da Bahia, Departamento de Ciências Exatas e da Terra, Campus II, Rodovia Alagoinhas/Salvador, BR-110, Km 03, Alagoinhas, Bahia, Brazil 35 Institute of Botany, Chinese Academy of Sciences, State Key Laboratory of Systematic and Evolutionary Botany, Beijing, 100093, China 36 Instituto Tecnológico Vale / Museu Paraense Emílio Goeldi (MPEG), Belém, Pará, Brazil

Author information KM, https://orcid.org/0000-0002-7658-0844; JK, https://orcid.org/0000-0002-9983-7630; EMdA, https:// orcid.org/0000-0001-8033-5334; LIFA, https://orcid.org/0000-0002-4182-911X; CAB, https://orcid.org/0000-0001-6412-6700; CMB, https://orcid.org/0000-0001-9546-5721; EVB, https://orcid.org/0000-0003-1314-8429; FRCB, https://orcid.org/0000-0003- 3868-1131; NIC, https://orcid.org/0000-0003-4739-6885; OAC, https://orcid.org/0000-0003-0801-8961; MCD, https://orcid.org/ 0000-0002-2981-0328; ASE, https://orcid.org/0000-0002-4844-0254; LPF, https://orcid.org/0000-0001-9202-9828; ERFM, https://orcid.org/0000-0002-8889-4955; MJGdA, https://orcid.org/0000-0003-3112-0649; PDG, https://orcid.org/0000-0002- 6537-8018; RCG, https://orcid.org/0000-0003-0913-9917; KK, https://orcid.org/0000-0002-4011-8666; MKA, https://orcid.org/ 0000-0003-4153-6728; AAK, https://orcid.org/0000-0002-7779-3438; VAK, https://orcid.org/0000-0002-5213-2166; VVK, https://orcid.org/0000-0003-1872-2223; DAK, https://orcid.org/0000-0003-2658-1723; IVK, https://orcid.org/0000-0002-6824- 4015; ZML, https://orcid.org/0000-0002-8284-1983; SSM, https://orcid.org/0000-0003-1975-6548; EMN, https://orcid.org/0000- 0002-8121-382X; ALM, https://orcid.org/0000-0003-4267-5818; EYuM, https://orcid.org/0000-0002-8487-5714; VON, http:// orcid.org/0000-0001-6756-2823; JALN, https://orcid.org/0000-0003-0220-0746; JRP, https://orcid.org/0000-0001-7984-4457; NSP, https://orcid.org/0000-0002-3279-4824; MVRdC, https://orcid.org/0000-0001-7146-0539; ERP, https://orcid.org/0000- 0003-2342-2049; MISS, https://orcid.org/0000-0002-1907-1015; FJS, https://orcid.org/0000-0003-1128-1138; GSS, https://orcid. org/0000-0002-9360-3729; PKS, https://orcid.org/0000-0003-1684-8893; VS, https://orcid.org/0000-0002-9404-2783; PCSSS, https://orcid.org/0000-0003-1122-1047; TS, https://orcid.org/0000-0002-0124-2453; APS, https://orcid.org/0000-0003-2220- 826X; HS, https://orcid.org/0000-0001-5127-4579; WGS, https://orcid.org/0000-0001-9707-2665; ANT, https://orcid.org/0000- 0002-1979-2568; MAT, https://orcid.org/0000-0002-0307-5919; OTN, https://orcid.org/0000-0001-6598-0511; EU, https://orcid. org/0000-0003-1375-3549; VLCV, https://orcid.org/0000-0002-4055-5049; MSV, https://orcid.org/0000-0002-3208-4857; WW, https://orcid.org/0000-0001-6901-6375; KX, https://orcid.org/0000-0002-0800-6015; DCZ, https://orcid.org/0000-0001-6755-2238 DOI https://doi.org/10.1002/tax.12156

IAPT chromosome data 30/1 Alstroemeria werdermannii var. flavicans Muñoz-Schick, 2n = 16; Chile, Atacama Region, C. Baeza & G. Kottirsch 4427 (CONC). Carlos M. Baeza,* Oscar Toro-Núñez, N. Ivalú Cacho, Alstroemeria werdermannii Ehr.Bayer var. werdermannii,2n = 16; Marely Cuba-Díaz, Estrella Urtubey, Eduardo Ruiz-Ponce, Chile, Atacama Region, C. Baeza & G. Kottirsch 4425 (CONC). Claudia Araneda-Beltrán & Tod Stuessy * Address for correspondence: [email protected] ASTERACEAE Chaetanthera albiflora (Phil.) A.M.R.Davies, 2n = 24; Chile, This study was funded by projects VRID No. 318/17 (Code Coquimbo Region, C. Baeza 4378 (CONC). 217.111.063-1.0) University of Concepcion, Chile, DGAPA- Duseniella patagonica (O.Hoffm.) K.Schum., 2n = 54; Argentina, PAPIIT-IA201516, CONICET PIP 114-20110100308, and VRID- Chubut, E. Urtubey & E.M. Baztarrica 955 (CONC, SI). Asociativo 217.418.009-1.0. BRASSICACEAE All materials CHN. Neuontobotrys tarapacana (Phil.) Al-Shehbaz, 2n = 24; Chile, Antofagasta Region, O. Toro & N.I. Cacho 143 (CONC). ALSTROEMERIACEAE Alstroemeria philippii subsp. adrianae J.M.Watson & A.R.Flores, CARYOPHYLLACEAE 2n = 16; Chile, Atacama Region, C. Baeza & G. Kottirsch Colobanthus quitensis (Kunth) Bartl., 2n = 80; Chile, Magallanes 4428 (CONC). and Chilean Antartic Region, 15 Jan 2015, M. Cuba-Díaz Alstroemeria philippii Baker var. philippii,2n = 16; Chile, Atacama s.n. (CONC). Region, P. Carrasco 107 (CONC).

Version of Record 1125 Marhold & Kucˇera (eds.) • IAPT chromosome data 30 TAXON 68 (5) • October 2019: 1124–1130

MALVACEAE Aquilegia lactiflora Kar. & Kir., 2n = 14; Tajikistan, Rushon, MO Tarasa reichei (Phil.) Krapov., 2n = 12; Chile, Maule Region, 15 Jan 13 (NS). 2016, C. Sepúlveda & P. Peñailillo s.n. (CONC). Aquilegia viridiflora Pall., 2n = 14; China, Gansu, YL 2 (NS). Eranthis hyemalis (L.) Salisb., 2n = 16; Russia, Leningradskaya Oblast’, AG 1 (NS). IAPT chromosome data 30/2 *Eranthis lobulata W.T.Wang, 2n = 16+0–1B; China, Sichuan, LZ 1 (NS). Andrey S. Erst,* Elizaveta Yu. Mitrenina, Alexander P. *Eranthis longistipitata Regel, 2n = 16; Kazakhstan, Jambyl Region, Sukhorukov, Igor V. Kuzmin, Polina D. Gudkova, VK 1 (NS). Alexander N. Tashev, Kunli Xiang & Wei Wang **Eranthis sibirica DC., 2n = 28; Russia, Khakassia Republic, AE * Address for correspondence: [email protected] & TE 711 (NS); Irkutskaya Oblast’, AE, DK, EM & OC 12 (NS). 2n = 42; Russia, Irkutskaya Oblast’, AE, DK & OC 13 (NS); The study was financially supported by the Russian Science Russia, Irkutskaya Oblast’, AE, DK, EM & OC 14 (NS); Russia, Foundation (Grant Nos. 18-34-20056 mol_a_ved and 18-04-00653 Irkutskaya Oblast’, AE, DK, EM & OC 15 (NS). A to Andrey Erst and Elizaveta Mitrenina [chromosome counts and Eranthis stellata Maxim., 2n = 16; Russia, Primorskii Krai, VY microphotographing] and Grant No. 18-34-20112 mol_а_ved to 12 (NS). Polina Gudkova [observations of the herbarium material in LE and Hegemone lilacina Bunge, 2n = 16; Russia, Altai Republic, AE 433 (NS). field work in Tajikistan]) and the Scientific program (Grant Trollius altaicus C.A.Mey., 2n = 16; Russia, Altai Republic, AE No. AAAA-A16-116021660045-2 to Alexander Sukhorukov [field 442 (NS). work in Africa]). Trollius asiaticus L., 2n = 16; Russia, Novosibirskaya Oblast’, AE 457 (NS). All materials CHN; collectors: AAE = A.A. Erst, AE = A.S. Erst, AG = A. Grebenjuk, AS = A. Sukhorukov, CS = C. Shi, DK = D. Kri- ROSACEAE venko, EM = E. Mitrenina, IK = I. Kuzmin, LB = L. Bing, LL = Rosa rugosa Thunb., 2n = 14; Russia, Northwestern Federal District, L. Lian, LZ = L. Zhang, MO = M. Olonova, OC = O. Chernysheva, IK 004 (NS). PG = P. Gudkova, TE = T. Erst, VK = V. Kolbintzev, VY = V. Yaku- Rubus crataegifolius Bunge, 2n = 14; China, Beijing, AE, TE, LL, LB bov, YL = Y. Luo. &CS23(NS).

* First chromosome count for the species. ** New cytotype for the species. IAPT chromosome data 30/3

ASPARAGACEAE Kuljit Kaur,* Vijay Singh & Raghbir Chand Gupta Asparagus officinalis L., 2n = 20; Russia, Novgorodskaya Oblast’, IK *Address for correspondence: [email protected] 001 (NS). Polygonatum odoratum (Mill.) Druce, 2n = 20; Russia, Tyumens- The study was supported by financial grant under IPLS Project kaya Oblast’, IK 246 (NS). of DBT (BT/PR 4548/INF/22/146/2012), UGC-RGNF (F1-17.1/ 2011-12/RGNF-SC-PUN-1224) and UGC-BSR (Reference number: BRASSICACEAE BT/PR 4548/INF/22/146/2012). Thlaspi arvense L., 2n = 14; Russia, Tyumenskaya Oblast’, IK 016 (NS). All materials CHN; collectors: KK = Kuljit Kaur; VS = Vijay Singh; vouchers in PUN. LAMIACEAE Lamium amplexicaule L., 2n = 18; Israel, Jerusalem city, AS BRASSICACEAE 001 (MW). Sisymbrium orientale L., n=18; India, Rajasthan, KK 59768.

NYCTAGINACEAE CACTACEAE *Boerhavia cordobensis Kuntze, 2n = 52; Namibia, Windhoek, AS Opuntia elatior Mill., n = 11; India, Rajasthan, KK 60371. 002 (MW). CAMPANULACEAE POACEAE Campanula latifolia L., n = 14; India, Himachal Pradesh, VS 60123. Panicum miliaceum subsp. ruderale (Kitag.) Tzvelev, 2n = 36; Cyananthus lobatus Wall. ex Benth., n=14; India, Himachal Russia, Tyumenskaya Oblast’, IK 017 (NS). Pradesh, VS 58810. Stipa glareosa P.A.Smirn., 2n = 44; Tajikistan, Gorno-Badakhshan Autonomous Region, PG & MO 1, PG & MO 2, PG & MO 3 CAPRIFOLIACEAE (TK). Lonicera hispida Pall. ex Schult., n=9; India, Himachal Pradesh, VS 60131. RANUNCULACEAE Morina longifolia Wall. ex DC., n=17; India, Himachal Pradesh, VS Aquilegia canadensis L., 2n = 14; U.S.A., North Carolina, AAE 60103. 018 (NS). Aquilegia ecalcarata Maxim., 2n = 14; China, Gansu, YL 1 CARYOPHYLLACEAE (NS). Arenaria serpyllifolia L., n=9; India, Rajasthan, KK 59766.

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FABACEAE Artemisia arenaria DC., 2n = 36; Russia, Kaliningradskaya Oblast’, Indigofera caerulea Roxb., n=8; India, Rajasthan, KK 60418. GYuK 2018-20; Russia, Republic of Kalmykia, RRD 2018-07, RRD Tephrosia candida DC., n=22; India, Rajasthan, KK 60377. 2018-08. Artemisia austriaca Jacq., 2n = 18; Russia, Republic of Kalmykia, GENTIANACEAE RRD 2018-11, RRD 2018-13. Jaeschkea gentianoides Kurz, n=10; India, Himachal Pradesh, VS Artemisia borealis Pall., 2n = 18; Russia, Tyumenskaya Oblast’, 60092. Yamalo-Nenetskii Avtonomnyi Okrug, N.N. Pospelov 2018-06. Artemisia campestris L., 2n = 36; Russia, Leningradskaya Oblast’, LAMIACEAE GYuK 2018-25, GYuK 2018-26; Russia, Kaliningradskaya Oblast’, Lycopus europaeus L., n=11+1B; India, Himachal Pradesh, VS GYuK 2018-19, GYuK 2018-21, GYuK 2018-23, GYuK 2018-24; 60058. Russia, Saint Petersburg city, 08 Nov 2017, A.A. Korobkov s.n. Artemisia commutata Besser, 2n = 18; Russia, Republic of Sakha OLEACEAE (Yakutia), DAK 2018-29.2n = 36; Russia, Republic of Buryatia, Syringa emodii Wall. ex. G.Don, n=22; India, Himachal Pradesh, NSG 2018-65, DAK 2018-42; Russia, Republic of Bashkortostan, VS 60030. A.A. Muldeshev 2018-63. Artemisia desertorum Spreng., 2n = 18; Russia, Republic of Sakha POLYGALACEAE (Yakutia), DAK 2018-31; Russia, Zabaikalskii Krai, TET 2017- Polygala erioptera DC., n=24; India, Rajasthan, KK 59693. 11, TET 2017-12, TET 2017-13. Artemisia dolosa Krasch., 2n = 36; Russia, Russia, Republic of Bur- SCROPHULARIACEAE yatia, DAK 2018-43, DAK 2018-44. Veronica persica Poir., n=16+0−1B; India, Himachal Pradesh, VS Artemisia dracunculus L., 2n = 18; Russia, Republic of Sakha (Yaku- 60094. tia), DAK 2018-30. Artemisia frigida Willd., 2n = 36; Russia, Republic of Buryatia, DAK VERBENACEAE 2018-47, DAK 2018-48. Verbena officinalis L., n=28; India, Himachal Pradesh, VS Artemisia jacutica Drobow, 2n = 18; Russia, Republic of Sakha 58664. (Yakutia), DAK 2018-32. Artemisia lerchiana Stechm., 2n = 18; Russia, Republic of Kalmykia, RRD 2018-10a.2n = 36; Russia, Republic of Kalmykia, RRD IAPT chromosome data 30/4 2018-10b. Artemisia lithophila Turcz. ex DC., 2n = 18; Russia, Republic of Bur- Aleksandr A. Korobkov, Violetta V. Kotseruba & yatia, NSG 2018-64. Denis A. Krivenko* Artemisia mongolica (Fisch. ex Besser) Nakai, 2n = 16; Russia, *Address for correspondence: [email protected] Republic of Sakha (Yakutia), DAK 2018-37. Artemisia pauciflora Weber ex Stechm., 2n = 18; Russia, Republic of We are grateful to the scientists of the Astrakhan Nature Reserve Kalmykia, RRD 2018-12. K.V. Litvinov and N.O. Meshcheryakova as well as scientist of the Artemisia phaeolepis Krasch., 2n = 36; Russia, Republic of Buryatia, Chornye Zemli Nature Reserve V.E. Badmaev and Professor of DAK 2018-45, DAK 2018-46. Kalmyk State University R.R. Dzhapova for help in collecting material. Artemisia santonicum L., 2n = 36; Russia, Republic of Kalmykia, The study of Asteraceae was funded by RFBR, no. 16-04-00052, and RRD 2018-09. the study of Ranunculaceae was funded by RSF, no. 19-74-10082. Artemisia sieversiana Ehrh. ex Willd., 2n = 18; Russia, Irkutskaya Oblast’, DAK 2018-49. All materials CHN; collectors: ASE = A.S. Erst, AT = A. Tashev, Artemisia vulgaris L., 2n = 16; Russia, Leningradskaya Oblast’, DAK = D.A. Krivenko, EMM = E.M. Machs, GYuK = G.Yu. Konech- GYuK 2018-27. naya, NSG = N.S. Gamova, RRD = R.R. Dzhapova, TET = Aster alpinus L., 2n = 18; Russia, Republic of Buryatia, DAK T.E. Tkachuk; vouchers of Asteraceae in LE, and vouchers of Ranun- 2018-51. culaceae in NS. Aster sibiricus L., 2n = 18; Russia, Republic of Sakha (Yakutia), DAK 2018-60. ASTERACEAE (COMPOSITAE) Chamomilla suaveolens (Pursh) Rydb., 2n = 18; Russia, Republic of Achillea asiatica Serg., 2n = 18; Russia, Republic of Sakha (Yaku- Sakha (Yakutia), DAK 2018-56. tia), DAK 2018-34. Dendranthema zawadskii (Herbich) Tzvelev, 2n = 72; Russia, Achillea micrantha Willd., 2n = 18; Russia, Astrakhanskaya Oblast’, Republic of Buryatia, DAK 2018-50. EMM 2018-04, EMM 2018-02, EMM 2018-03; Russia, Republic of Heteropappus altaicus (Willd.) Novopokr., 2n = 18; Russia, Repub- Kalmykia, EMM 2018-01. lic of Sakha (Yakutia), DAK 2018-54; Russia, Republic of Bur- Achillea millefolium L., 2n = 36; Russia, Leningradskaya Oblast’, yatia, DAK 2018-52. V.I. Dorofeev 2017-08; Russia, Republic of Sakha (Yakutia), DAK Ptarmica alpina (L.) DC., 2n = 36; Russia, Republic of Sakha (Yaku- 2018-39. tia), DAK 2018-41, DAK 2018-33. Antennaria dioica (L.) Gaertn., 2n = 18; Russia, Republic of Sakha Tanacetum boreale Fisch. ex DC., 2n = 18; Russia, Republic of (Yakutia), DAK 2018-58. Sakha (Yakutia), DAK 2018-35, DAK 2018-36. Artemisia absinthium L., 2n = 18; Russia, Kaliningradskaya Tripleurospermum inodorum (L.) Sch.Bip., 2n = 18; Russia, Astra- Oblast’, GYuK 2018-22; Russia, Leningradskaya Oblast’, GYuK khanskaya Oblast’, EMM 2018-05.2n = 36; Russia, Republic of 2018-28. Sakha (Yakutia), DAK 2018-40.

Version of Record 1127 Marhold & Kucˇera (eds.) • IAPT chromosome data 30 TAXON 68 (5) • October 2019: 1124–1130

RANUNCULACEAE BRASSICACEAE Anemone nemorosa L., 2n = 30; Bulgaria, ASE & AT 02. Draba hirta L., 2n = 64; Russia, Kamchatka, O.A. Chernyagina Anemone ranunculoides L., 2n = 32; Bulgaria, ASE & AT 01. 13346. Eranthis stellata Maxim., 2n = 16; Russia, Primorskii Krai, V.Yu. Nikulin & A.Yu. Nikulin 01. CARYOPHYLLACEAE Trollius altaicus C.A.Mey., 2n = 16; Russia, Republic of Altai, ASE Melandrium album (Mill.) Garcke, 2n = 24; Russia, Kamchatka, & T.V. Erst 128. O.A. Chernyagina 13322.

CONVOLVULACEAE IAPT chromosome data 30/5 Convolvulus chinensis Ker Gawl., 2n = 48; Russia, Republic of Bur- yatia, D.A. Krivenko 13356. Viktor O. Nachychko Address for correspondence: [email protected] GERANIACEAE Geranium dissectum L., 2n = 22; Sweden, O.A. Chernyagina The study was financially supported by research grants from Ivan 13405. Franko National University of Lviv, Ukraine for the support of funda- Geranium pratense L., 2n = 28; Russia, Irkutskaya Oblast’, O.Yu. mental research at Lviv University (projects PNS-010515, PN-010115). Zavgorodnyaya 13421.

All materials CHN; collected in Ukraine; collector: VN = Viktor LAMIACEAE O. Nachychko. Prunella vulgaris L., 2n = 28; Sweden, O.A. Chernyagina 13401.

LAMIACEAE PAPAVERACEAE Salvia glutinosa L., n =8,2n = 16; VN 1428 (CWU, KW, LWKS). Chelidonium asiaticum (Hara) Krahulc., 2n = 10; Russia, Primorskii Thymus alpestris Tausch ex A.Kern., 2n = 28; VN 1374 (CWU, Krai, G.G. Probatova 13301. KW, LWKS). Thymus alternans Klokov, 2n = 56; VN 1271 (CWU, KW, LWKS). POACEAE Thymus moldavicus Klokov & Des.-Shost., 2n = 28; VN 1038 (KW, Agrostis clavata Trin., 2n = 42; Russia, Kamchatka, O.A. Chernya- LWKS, WU). gina 13395. Agrostis geminata Trin., 2n = 28; Russia, Kamchatka, O.A. Chernya- gina 13402. IAPT chromosome data 30/6 Arrhenatherum elatius (L.) P.Beauv. ex J.Presl & C.Presl, 2n = 28; Sweden, O.A. Chernyagina 13403. Nina S. Probatova* & Olga A. Chernyagina Beckmannia syzigachne (Steud.) Fernald, 2n = 14; Russia, Kam- *Address for correspondence: [email protected] chatka, O.A. Chernyagina 13385. Elymus pendulinus (Nevski) Tzvelev, 2n = 28; Russia, Primorskii The study was supported by the Federal Agency for Krai, E.B. Volynets 13283. Scientific Organizations program for support the bioresource Festuca kolymensis Drobow, 2n = 42; Russia, Kamchatka, V.V. Yaku- collections. bov 13324, 13318. Hordeum jubatum L., 2n = 28; Russia, Altaiskii Krai, D.A. Krivenko All materials CHN; vouchers in VLA. 13409. Poa alpigena Lindm., 2n = 56; Russia, Kamchatka, O.A. Chernya- ACTINIDIACEAE gina 13393. Actinidia polygama (Siebold & Zucc.) Maxim., 2n = 58; Russia, Pri- Poa fischeri Prob., 2n = 28; Russia, Kamchatka, O.A. Chernyagina morskii Krai, E.B. Volynets 13350. 13333. Poa urssulensis Trin., 2n = 28; Russia, Kamchatskii Krai, V.V. Yaku- ASTERACEAE bov 13319. Artemisia stelleriana Besser, 2n = 18; Russia, Kamchatka, O.A. Schedonorus pratensis (Huds.) P.Beauv., 2n = 14; Russia, Kam- Chernyagina 13410. chatka, O.A. Chernyagina 13400. Bidens kamtschatica Vassilcz., 2n = 48; Russia, Kamchatka, O.A. Chernyagina 13349. PRIMULACEAE Bidens parviflora Willd., 2n = 24; Russia, Primorskii Krai, N.S. Pro- Androsace septentrionalis L., 2n = 20; Russia, Kamchatka, O.A. batova & G.G. Probatova 13298. Chernyagina 13321. Leontopodium kamtschaticum Kom., 2n = 26; Russia, Kamchatka, Primula farinosa L., 2n = 18; Russia, Kamchatka, O.A. Chernyagina Korablëv 13335. 13399. Picris kamtschatica Ledeb., 2n = 10; Russia, Kamchatka, O.A. Cher- nyagina 13323. RANUNCULACEAE Solidago canadensis L., 2n = 18; Sweden, O.A. Chernyagina Hepatica nobilis Mill., 2n = 14; Sweden, O.A. Chernyagina 13344. 13328. Solidago virgaurea L., 2n = 18; Sweden, O.A. Chernyagina 13336. VIOLACEAE Taraxacum natschikense Kom., 2n = 24; Russia, Kamchatka, O.A. Viola tricolor L., 2n = 26; Russia, Kamchatka, O.A. Chernyagina Chernyagina 13326. 13327.

1128 Version of Record TAXON 68 (5) • October 2019: 1124–1130 Marhold & Kucˇera (eds.) • IAPT chromosome data 30

IAPT chromosome data 30/7 IAPT chromosome data 30/9

María V.Romero-da Cruz,* José Rubens Pirani & Eliana Regina Fabiano J. Santos, Géssica S. Santos, Pamela C.S.S. Souza, Forni Martins Samara S. Matos, André L. Melo, Vera L.C. Vale & *Address for correspondence: [email protected] Maria J. Gomes de Andrade* *Address for correspondence: [email protected] Financial support from Brazilian agency: Fundação de Amparo à Pesquisa do Estado de São Paulo (Grants 2016/065724 and Financial support from Coordenação de Aperfeiçoamento de 2014/180022). Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvol- vimento Científico e Tecnológico (CNPq), and Fundação de Apoio à RUTACEAE Pesquisa do Estado da Bahia (FAPESB, process no. PIE0023/2016 Esenbeckia irwiniana Kaastra, 2n=64; Brasil, Minas Gerais, P. Dias for M.J.G. Andrade). 526 (ESA). Esenbeckia leiocarpa Engl., 2n=64; Brasil, São Paulo, E.R.F. All materials CHN; vouchers in HUNEB. Martins & al. 30230 (UEC). Metrodorea concinna Pirani & P.Dias, 2n=ca. 54; Brasil, Bahia, LEGUMINOSAE P. Dias 557 (ESA). Subfamily Papilionoideae Tribe Dalbergieae (DC.) Cardoso & al. Zornia leptophylla (Benth.) Pittier, 2n = 20; Brazil, Bahia, F.J. San- IAPT chromosome data 30/8 tos 56. Tribe Indigofereae (Benth.) Hutch. Manjit Inder Singh Saggoo & Mandeep Kaur Aulakh* Indigofera hirsuta L., 2n = 16; Brazil, Pernambuco, F.J. Santos 19. *Address for correspondence: [email protected] Indigofera microcarpa Desv., 2n = 16; Brazil, Bahia, F.J. Santos 54. Indigofera suffruticosa Mill., 2n = 16; Brazil, Bahia, G.S. Santos 115. The study was supported by financial grant under DSA I of Tribe Millettieae Miq. UGC and UGC providing Senior Research Fellowship financial Lonchocarpus sericeus (Poir.) Kunth ex DC., 2n = 22; Brazil, Per- assistance to Mandeep Kaur Aulakh under BSR scheme for meritori- nambuco, F.J. Santos 13. ous students [award letter no. UGC/BSR F.5365]. Tephrosia purpurea (L.) Pers., 2n = 22; Brazil, Bahia, F.J. Santos 48. Tribe Phaseoleae (Bronn) DC. All materials CHN; collector: Mandeep Kaur Aulakh. Ancistrotropis peduncularis (Fawc. & Rendle) A.Delgado, 2n = 26; Brazil, Bahia, G.S. Santos 172. ADIANTACEAE Centrosema pascuorum Mart. ex Benth., 2n = 22; Brazil, Bahia, G.S. Adiantum capillus-veneris L., n = 30; India, Haryana (PUN 4827). Santos 134. n = 30+1B; India, Himachal Pradesh (PUN 4834). Clitoria fairchildiana R.A.Howard, 2n = 22; Brazil, Bahia, F.J. San- Adiantum caudatum L., n = 30; (PUN 4873). tos 01. Adiantum lunulatum Burm.f., n = 90; (PUN 4848). Macroptilium atropurpureum (DC.) Urb., 2n = 22; Brazil, Bahia, F.J. Santos 51. ASPLENIACEAE Macroptilium lathyroides (L.) Urb., 2n = 22; Brazil, Bahia, G.S. San- Asplenium trichomanes L., n = 36; India, Himachal Pradesh (PUN tos 110. 4875). n = 72; India, Himachal Pradesh (PUN 4876). n = 72+1B; Rhynchosia minima (L.) DC., 2n = 22; Brazil, Pernambuco, F.J. San- India, Himachal Pradesh (PUN 4876). tos 45.

DRYOPTERIDACEAE Polystichum squarrosum (D.Don) Fée, n = 41; India, Himachal Pra- IAPT chromosome data 30/10 desh (PUN 4877). 2n =3x = 123; India, Himachal Pradesh (PUN 4849). 2n =3x = 123+1B; India, Himachal Pradesh (PUN 4850). Pollyana K. Silva, Lânia I.F. Alves,* José Achilles L. Neves, Erton Mendonça de Almeida, Enoque Medeiros Neto, PTERIDACEAE Fabiane R.C. Batista, Daniela C. Zappi & Leonardo P. Felix Pteris cretica L., 2n = 58; India, Himachal Pradesh (PUN 4839). 2n *Address for correspodence: [email protected] = 58+1B; India, Himachal Pradesh (PUN 4840). 2n =3x = 87+1B; India, Himachal Pradesh (PUN 4842). Financial support from CNPq (Conselho Nacional de Des- envolvimento Científico e Tecnológico), UFPB (Universidade Fe- THELYPTERIDACEAE deral da Paraíba), INSA (Instituto Nacional do Semiárido). Glaphyropteridopsis erubescens (Wall.ex Hook.) Ching, n = 36; India, Himachal Pradesh (PUN 4874). All materials CHN; collected by EMA = E.M. Almeida & al.; vouchers in EAN (Herbário Prof. Jayme Coelho de Moraes).

CACTACEAE Subfamily Cactoideae, Tribe Cereeae Coleocephalocereus aureus F.Ritter, 2n = 22; Brazil, Minas Gerais, EMA 1970.

Version of Record 1129 Marhold & Kucˇera (eds.) • IAPT chromosome data 30 TAXON 68 (5) • October 2019: 1124–1130

Coleocephalocereus decumbens F.Ritter, 2n = 22; Brazil, Minas Ger- Saussurea velutina W.W.Sm., 2n =2x = 30; China, Yunnan, XM & ais, EMA 2025. WS 17-747. Coleocephalocereus goebelianus (Vaupel) Buining, 2n = 22; Brazil, Soroseris erysimoides (Hand.-Mazz.) C.Shih, 2n =4x = 32; China, Bahia, EMA 2091. Yunnan, JZ, YZ & QG 224. Coleocephalocereus purpureus (Buining & Brederoo) F.Ritter, 2n Soroseris gillii (S.Moore) Stebbins, 2n =2x = 16; China, Tibet, JZ, = 22; Brazil, Minas Gerais, EMA 2013. YZ & QG 217. Pilosocereus brasiliensis subsp. ruschianus (Buining & Brederoo) Soroseris glomerata (Decne.) Stebbins, 2n =4x = 32; China, Yun- Zappi, 2n = 22; Brazil, Bahia, EMA 1965. nan, HS SunH-07ZX-3365. Pilosocereus catingicola (Gürke) Byles & G.D.Rowley subsp. catin- Soroseris hirsuta (J.Anthony) C.Shih, 2n =2x = 16; China, Sichuan, gicola,2n = 44; Brazil, Bahia, EMA 1760. JZ 026. Pilosocereus catingicola subsp. salvadorensis (Werderm.) Zappi, 2n Soroseris hookeriana Stebbins, 2n =4x = 32; China, Sichuan, JZ, BY = 44; Brazil, Paraíba, EMA 1616. & HC 934. Pilosocereus chrysostele (Vaupel) Byles & G.D.Rowley, 2n = 22; Stebbinsia umbrella (Franch.) Lipsch., 2n =2x = 16; China, Yunnan, Brazil, Paraíba, EMA 1613. HS SunH-07ZX-3421. China, Sichuan, JZ, BY & HC 931, JZ, YZ & Pilosocereus floccosus subsp. quadricostatus (F.Ritter) Zappi, 2n QG 019. = 22; Brazil, Minas Gerais, EMA 1971. Syncalathium chrysocephalum (C.Shih) C.Shih, 2n =2x = 16; China, Pilosocereus glaucochrous (Werderm.) Byles & G.D.Rowley, 2n Tibet, JZ, WS & LQ 193, JZ, WS & LQ 217. = 22; Brazil, Bahia, EMA 1755. Syncalathium disciforme (Mattf.) Y.Ling, 2n =2x = 16; China, Qin- Pilosocereus gounellei (F.A.C.Weber ex K.Schum.) Byles & hai, JZ, WS & LQ 309, JZ, WS & LQ 327; China, Sichuan, JZ, WS & G.D.Rowley subsp. gounellei,2n = 22; Brazil, Paraíba, EMA 1591. LQ 466, JZ, WS & LQ 530. Pilosocereus gounellei subsp. zehntneri (Britton & Rose) Zappi, 2n Syncalathium kawaguchii (Kitam.) Y.Ling, 2n =2x = 16; China, = 22; Brazil, Bahia, EMA 1777. Tibet, JZ 3431, JZ 3435, JZ 3366, JZ 3305, JZ 4081, JZ 3425, Pilosocereus magnificus (Buining & Brederoo) F.Ritter, 2n = 22; JZ 3412. Brazil, Minas Gerais, EMA 2011. Youngia paleacea (Diels) Babc. & Stebbins, 2n =2x = 16; China, Pilosocereus multicostatus F.Ritter, 2n = 22; Brazil, Minas Gerais, Yunnan, HC & JY 048. EMA 1976. Youngia simulatrix (Babc.) Babc. & Stebbins, 2n = 16; China, Tibet, Pilosocereus pachycladus F.Ritter subsp. pachycladus,2n = 44; Bra- JZ 3436. zil, Minas Gerais, EMA 2000. Pilosocereus pentaedrophorus (Labour.) Byles & G.D.Rowley BRASSICACEAE subsp. pentaedrophorus,2n = 22; Brazil, Pernambuco, EMA 1654. Draba oreodoxa W.W.Sm., 2n =2x = 24; China, Yunnan, JY 053. Pilosocereus tuberculatus (Werderm.) Byles & G.D.Rowley, 2n = 22; Brazil, Pernambuco, EMA 1681. LAMIACEAE Salvia flava Forrest ex Diels, 2n =2x = 16; China, Sichuan, WS 2237. IAPT chromosome data 30/11 Salvia przewalskii Maxim., 2n =4x = 32; China, Yunnan, WS 37151.

Wen-Guang Sun, Hang Sun* & Zhi-Min Li* *Addresses for correspondence: Z.M. Li, [email protected]; IAPT chromosome data 30/12 H. Sun, [email protected] Mariya S. Voronkova, Evgeny V.Banaev,* Mariya A. Tomoshevich This work was supported by grants from the National Natural & Vera A. Kostikova Science Foundation of China (grant numbers 31670206, *Address for correspondence: [email protected] 31960046), Strategic Priority Research Program of Chinese Acad- emy of Sciences (grant number XDA 20050203), and NSFC-Yunnan All materials CHN; collectors: DKK & VAK = D.K. Kostikov joint fund to support key projects (grant number U1802232). We & V.A. Kostikova; vouchers in NSK. would like to thank Dr. Xiang-Guang Ma, Dr. Jian-Wen Zhang and Dr. Yong-Hong Zhang for their help during field research. POLYGONACEAE Atraphaxis frutescens (L.) K.Koch, 2n = 16, 18, 20, 22; Russia, All materials CHN; collectors: BY = B. Yang, HC = H.L. Chen, Republic of Tuva, DKK & VAK 3000905.2n = 16, 32, 56; Russia, HS = H. Sun, JY = J.P. Yue, JZ = J.W. Zhang, LQ = L.S. Qian, QG = Novosibirskaya Oblast’, DKK & VAK 3000901.2n = 18, 22; Rus- Q.B. Gong, TT = T.Y. Tu, WS = W.G. Sun, XM = X.G. Ma, YZ = sia, Republic of Tuva, DKK & VAK 3000906.2n = 22; Russia, Y.H. Zhang; vouchers in KUN. Republic of Khakasiya, DKK & VAK 3000904.2n = 48, 50, 52; Russia, Omskaya Oblast’, DKK & VAK 3000902.2n = 48, 64, 80; ASTERACEAE Russia, Altaiskii Krai, 3000911.2n = 58, 62, 64; Russia, Altaiskii Crepis bodinieri H.Lév., 2n =4x = 32; China, Yunnan, JZ 1043. Krai, DKK & VAK 3000903. Parasyncalathium souliei (Franch.) J.W.Zhang, Boufford & H.Sun, Atraphaxis pungens (M.Bieb.) Jaub. & Spach, 2n = 18, 20; Russia, 2n =2x = 16; China, Sichuan, JZ, YZ & QG 264, JZ, YZ & QG Republic of Tuva, DKK & VAK 3000908.2n = 18, 20, 22; 180; China, Tibet, JZ, YZ & QG 215, HC & JY 003, JZ, YZ & Russia, Republic of Tuva, DKK & VAK 3000909.2n = 42, 44, QG 234; China, Yunnan, HS SunH-07zx-2821. 48, 52, 62, 64, 66, 68; Russia, Republic of Altai, DKK & VAK Saussurea obvallata (DC) Sch.Bip., 2n =2x = 16; China, Tibet, JZ & 3000910.2n = 62, 66, 68, 72; Russia, Republic of Tuva, DKK & TT 122. VAK 3000907.

1130 Version of Record Marhold & Kucˇera (eds.) • IAPT chromosome data 30 TAXON 68 (5) • October 2019: E1–E29

IAPT CHROMOSOME DATA

IAPT chromosome data 30 – Extended version Karol Marhold (ed.),1,2 Jaromír Kucˇera (ed.),1 Erton Mendonça de Almeida,3 Lânia I.F. Alves,4 Claudia Araneda-Beltrán,5 Carlos M. Baeza,5 Evgeny V. Banaev,6 Fabiane R.C. Batista,4 N. Ivalú Cacho,7 Olga A. Chernyagina,8 Marely Cuba-Díaz,9 Andrey S. Erst,6,10 Leonardo P. Felix,11 Eliana Regina Forni Martins,12 Maria J. Gomes de Andrade,13 Polina D. Gudkova,10,14 Raghbir Chand Gupta,15 Kuljit Kaur,15 Mandeep Kaur Aulakh,15 Aleksandr A. Korobkov,16 Vera A. Kostikova,6 Violetta V. Kotseruba,16 Denis A. Krivenko,10,17,18 Igor V. Kuzmin,19 Zhi-Min Li,20 Samara S. Matos,13 Enoque Medeiros Neto,11 André L. Melo,21 Elizaveta Yu. Mitrenina,10 Viktor O. Nachychko,22 José Achilles L. Neves,11 José Rubens Pirani,23 Nina S. Probatova,24 María V. Romero-da Cruz,23 Eduardo Ruiz-Ponce,5 Manjit Inder Singh Saggoo,15 Fabiano J. Santos,25 Géssica S. Santos,13 Pollyana K. Silva,4 Vijay Singh,15 Pamela C.S.S. Souza,25 Tod Stuessy,26,27 Alexander P. Sukhorukov,28 Hang Sun,29 Wen-Guang Sun,29,30,31 Alexander N. Tashev,32 Mariya A. Tomoshevich,6 Oscar Toro-Núñez,5 Estrella Urtubey,33 Vera L.C. Vale,34 Mariya S. Voronkova,6 Wei Wang,35 Kunli Xiang35 & Daniela C. Zappi36 1 Plant Science and Biodiversity Centre, Institute of Botany, Slovak Academy of Sciences, Dubravska cesta 9, 845 23 Bratislava, Slovak Republic 2 Department of Botany, Charles University, Benatska 2, 128 01 Praha, Czech Republic 3 Laboratory of plant Cytogenetics and Evolution, Department of Botany, Federal University of Pernambuco, Recife, Pernambuco, Brazil 4 Laboratório de Citogenética Vegetal, Instituto Nacional do Semiárido - INSA, Campina Grande, Paraíba, Brazil 5 Departamento de Botánica, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile 6 Central Siberian Botanical Garden SB RAS, Zolotodolinskaya Str. 101, 630090 Novosibirsk, Russia 7 Instituto de Biología, Universidad Nacional Autónoma de México, CDMX 04510, México 8 Kamchatka Branch of the Pacific Geographical Institute, FEB RAS, 6 Partizanskaya Str., 683000, Petropavlovsk-Kamchatskii, Russia 9 Laboratorio de Biotecnología y Estudios Ambientales, Departamento de Ciencias y Tecnología Vegetal, Escuela de Ciencias y Tecnologías, Campus Los Ángeles, Universidad de Concepción, Chile 10 National Research Tomsk State University, Laboratory of Herbarium, Lenin Ave. 36, Tomsk 634050, Russia 11 Department of Biological Sciences, Federal University of Paraíba, Areia, Paraíba, Brazil 12 Instituto de Biologia, Universidade Estadual de Campinas – UNICAMP, Brasil 13 Programa de Pós-Graduação em Biodiversidade Vegetal, Universidade do Estado da Bahia, Departamento de Educação, Campus VIII, Rua da Gangorra, 503, CHESF 48608-240, Paulo Afonso, Bahia, Brazil 14 Department of Biology, Altai State University, Lenin Ave. 61, Barnaul 656049, Russia 15 Department of Botany, Punjabi University, Patiala, 147002, India 16 V.L. Komarov Botanical Institute of the Russian Academy of Sciences, Prof. Popov Str. 2, 197376 St. Petersburg, Russia 17 Siberian Institute of Plant Physiology & Biochemistry of the Siberian Branch of the Russian Academy of Sciences, Lermontov Str. 132, 664033 Irkutsk, Russia 18 Western Baikal Protected Areas, Baikalskaya Str. 291B, 664050 Irkutsk, Russia 19 Tyumen State University, Department of Botany, 10 Semakova str., Tyumen 625023, Russia 20 School of Life Science, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Yunnan Normal University, Kunming, China 21 Universidade Federal Rural de Pernambuco, Unidade Acadêmica de Serra Talhada, Avenida Gregório Ferraz Nogueira, S/N, José Tomé de Souza Ramos, 56909-535, Serra Talhada, Pernambuco, Brazil 22 Botanical Garden of Ivan Franko National University of Lviv, 44 Cheremshyna Str., Lviv 79014, Ukraine 23 Instituto de Biociências, Universidade de São Paulo - USP, Brasil 24 Federal Scientific Center of the East Asian Terrestrial Biodiversity, FEB RAS, 159 Stoletya Prospect, 690022, Vladivostok, Russia 25 Universidade do Estado da Bahia, Departamento de Educação, Campus VIII, Rua da Gangorra, 503, CHESF 48608-240, Paulo Afonso, Bahia, Brazil 26 Herbarium and Department of Evolution, Ecology, and Organismal Biology, The Ohio State University, 1315 Kinnear Road, Columbus, Ohio 43212, U.S.A. 27 Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, 1030 Vienna, Austria 28 Lomonosov Moscow State University, Department of Higher Plants, Biological Faculty, Moscow 119234, Russia 29 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China

Version of Record E1 Marhold & Kucˇera (eds.) • IAPT chromosome data 30 TAXON 68 (5) • October 2019: E1–E29

30 School of Life Science, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Yunnan Normal University, Kunming, China 31 University of Chinese Academy of Sciences, Beijing, China 32 Department of Dendrology, University of Forestry, 1797 Sofia, Bulgaria 33 Instituto de Botánica Darwinion, Labardén 200, San Isidro, Buenos Aires, Argentina 34 Programa de Pós-Graduação em Biodiversidade Vegetal, Universidade do Estado da Bahia, Departamento de Ciências Exatas e da Terra, Campus II, Rodovia Alagoinhas/Salvador, BR-110, Km 03, Alagoinhas, Bahia, Brazil 35 Institute of Botany, Chinese Academy of Sciences, State Key Laboratory of Systematic and Evolutionary Botany, Beijing, 100093, China 36 Instituto Tecnológico Vale / Museu Paraense Emílio Goeldi (MPEG), Belém, Pará, Brazil

IAPT chromosome data 30/1 There is a previous count of Buitendijk & Ramanna (1996) for this species, but it is unclear to which variety it refers to. It is likely Carlos M. Baeza,* Oscar Toro-Núñez, N. Ivalú Cacho, to have been from the most abundant, typical variety. Marely Cuba-Díaz, Estrella Urtubey, Eduardo Ruiz-Ponce, Claudia Araneda-Beltrán & Tod Stuessy **Alstroemeria werdermannii var. flavicans Muñoz-Schick *Address for correspondence: [email protected] 2n = 16, CHN. Chile, Atacama Region, Punta de Choros, 15 m, 2914′50″S, 7128′00″W, 19 Sep 2017, C. Baeza & G. Kottirsch This study was funded by projects VRID No. 318/17 (Code 4427 (CONC) [Fig. 1C]. 217.111.063-1.0) University of Concepcion, Chile, DGAPA- PAPIIT-IA201516, CONICET PIP 114-20110100308, and VRID- **Alstroemeria werdermannii Ehr.Bayer var. werdermannii Asociativo 217.418.009-1.0. 2n = 16, CHN. Chile, Atacama Region, road to Carrizalillo, km 35, 313 m, 2856′55.1″S, 7117′00.6″W, 19 Sep 2017, C. Baeza * First chromosome count for genus and species. & G. Kottirsch 4425 (CONC) [Fig. 1D]. ** First chromosome count for species. Alstroemeria werdermannii is a Chilean species consisting of ALSTROEMERIACEAE two varieties, both very restricted along the coast in the regions of **Alstroemeria philippii subsp. adrianae J.M.Watson & Atacama and Coquimbo in northern Chile (Finot & al., 2018). A.R.Flores 2n = 16, CHN. Chile, Atacama Region, alongside road La ASTERACEAE Higuera–La Serena, at km 503, close to Juan Soldado locality, Chaetanthera albiflora (Phil.) A.M.R.Davies 180 m, 2939′00″S, 7118′06″W, 20 Sep 2017, C. Baeza & G. Kot- 2n = 24, CHN. Chile, Coquimbo Region, Limarí Province, Fray tirsch 4428 (CONC) [Fig. 1A]. Jorge National Park, 259 m, 3038′28″S, 7139′38″W, 31 Oct 2013, C. Baeza 4378 (CONC) [Fig. 1E]. Alstroemeria philippii Baker var. philippii There is a previous count of 2n = 24 by Grau (1987), under the 2n = 16, CHN. Chile, Atacama Region, Comuna de Huasco, name Ch. linearis var. albiflora Phil. dry gully 3 km north of the Bronces wharf, 183 m, 2835′09″S, 7117′15″W, 09 Oct 2017, P. Carrasco 107 (CONC) [Fig. 1B].

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*Duseniella patagonica (O. Hoffm.) K.Schum. Neuontobotrys O.E.Schulz belongs to Thelypoideae, a Brassica- 2n = 54, CHN. Argentina, Chubut, Escalante Department, Pro- ceae tribe with amphitropical distribution in America (Warwick vincial Route 26, 12.6 km of crossroad with National Route & al., 2009) and lacking cytological and caryological information 3, 205 m, 4553′04.9″S, 6743′37.4″W, 30 Nov 2014, E. Urtubey for most of its South American representatives (consulted from Bras- & E.M. Baztarrica 955 (CONC, SI) [Fig. 1F]. sibase by 25 Apr 2019; Koch & al. 2012; Kiefer & al., 2014). Neuon- The monotypic genus Duseniella K.Schum. belongs to the subfam- tobotrys tarapacana is a distinctive species from the Andes, typically ily Barnadesioideae, the basal linage of the family Asteraceae. This taxon found in the Altiplano-Patagonian areas of southern Peru and north- is a little annual plant with fleshy leaves and is endemic to Argentina. ern Argentina and Chile (Al-Shehbaz, 2006). Duseniella patagonica is closely related to Chuquiraga Juss. (shrubs, ca. 25 spp.) and Doniophyton Wedd. (subshrubs, 2 spp.), and they con- CARYOPHYLLACEAE stitute a monophyletic group in the phylogeny of the subfamily (Stuessy Colobanthus quitensis (Kunth) Bartl. & al., 1996; Urtubey & Stuessy, 2001; Gruenstaeudl & al., 2009). The 2n = 80, CHN. Chile, Magallanes and Chilean Antartic Region, chromosome numbers of Barnadesioideae are only partially known: King George Island, 3–23 m, 6209′S, 5828′W, 15 Jan 2015, Arnaldoa Cabrera, n =24–27 (A. weberbaueri (Muschl.) Ferreyra; M. Cuba-Díaz s.n. (CONC) [Fig. 1H]. Stuessy & Sagastegui, 1993); Barnadesia Mutis ex L.f., n = 12, 24II This area has a high diversity of mosses and lichens along (Olsen, 1980; Strother & Panero, 1994), 2n = ca. 48, 50, 52+0–2, with high abundance of the native species C. quitensis and 52, 62 (Cristóbal, 1986; Wulff, 1995; Stuessy & al., 1996), Chuquiraga, Deschampsia antarctica E.Desv. The habitat is characterized by n = 27, 54, 2n = 54, 108 (Wulff, 1990), Dasyphyllum Kunth, 2n =54 sandy soil and a predominance of strong winds and temperatures (Watanabe & al., 2007), Doniophyton, n = 24, 25, 2n = 48, 50 (Wulff, ranging from −10C to +15C. Due to the climatic conditions, it 1984, 1990), Schlechtendalia Less., n = 8 (Hunziker & al., 1989), 2n = has been found that the soils have a low water availability 16 (Cialdella & López de Kiesling, 1981). A base number of x =9has (Molina-Montenegro & al., 2012). There is a previous chromosome been proposed for the family Asteraceae, largely because this is the count for this species by Moore (1967), which indicates 2n = modal number in many species of different tribes (Solbrig, 1977), but ca. 80 (as C. crassifolius (d’Urv.) Hook.f. = C. quitensis, Rodriguez the x = 8 and 12 in Barnadesioideae (Stuessy & al., 2009) allow inferring & al., 2018). This new chromosome count confirms the previous polyploidy in the subfamily as in Chuquiraga, Doniophyton and Dasy- report by Moore (1967). phyllum. The chromosomal number counted for Duseniella patagonica (2n = 54) matches those registered for some species of Chuquiraga MALVACEAE and Dasyphyllum. Taking into consideration the basic chromosomal **Tarasa reichei (Phil.) Krapov. numbers for Barnadesioideae of x = 8 and 12, it is probable that multiple 2n = 12, CHN. Chile, Maule Region, Linares Province, Longaví, polyploidization events in D. patagonica took place during its evolution. 2.6 km northwest of the town, 150 m, 3558′46″S, 7131′10″W, 15 Jan 2016, C. Sepúlveda & P. Peñailillo s.n. (CONC) [Fig. 1I]. BRASSICACEAE Tarasa reichei is an endemic shrub of Chile (Marticorena, *Neuontobotrys tarapacana (Phil.) Al-Shehbaz 2005), known at the end of the 19th century only from its type locality 2n = 24, CHN. Chile, Antofagasta Region, Loa Province, San in the province of Talca, Maule Region. The present known distribu- Pedro de Atacama District, road to Paso Jama, besides main road, within tion area of the species ranges from San Pedro de Alcántara in the crevices and dry water beds, 3115 m, 2254′40.4″S, 6759′39.9″W, province of Cardenal Caro (O’Higgins Region) to the slope of 09 Dec 2017, O. Toro & N.I. Cacho 143 (CONC) [Fig. 1G]. Batuco, Pencahue (Maule Region).

Fig. 1. A, Alstroemeria philippii var. adrianae,2n = 16; B, Alstroemeria philippii var. philippii,2n = 16; C, Alstroe- meria werdermannii var. flavicans,2n = 16; D, Alstroemeria werdermannii var. werdermannii,2n = 16; E, Chae- tanthera albiflora,2n = 24; F, Duse- niella patagonica,2n = 54: G, Neuonto- botrys tarapacana,2n = 24; H, Colo- banthus quitensis,2n = 80; I, Tarasa reichei,2n = 12. — Scale bars = 5 μm.

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METHODS Moore, D.M. 1967. Chromosome numbers of Falkland Islands Rhizome roots or germinated seeds (1–2 cm length) obtained angiosperms. Brit. Antarct. Surv. Bull. 14: 69–82. from individuals in each population and held in a greenhouse were Olsen, J. 1980. [Report]. In: Löve, Á. (ed.), Chromosome number cut and pre-treated with a solution of 8-hydroxyquinoline (2 mM) reports LXVII. Taxon 29: 366–367. https://doi.org/10.1002/j. for 24 h at 4C. These samples were subsequently fixed with a fresh 1996-8175.1980.tb00677.x solution of ethanol/acetic acid (3 : 1) for 24 h. Squash preparations Rodríguez, R., Marticorena, C., Alarcón, D., Baeza, C., Cavieres, from root tips were made using an acid hydrolysis pretreatment with L., Finot, V., Fuentes, N., Kiesling, A., Mihoc, M., Pauchard, HCl 0.5 N during 17 min at 42C. After washing in distilled water, A., Ruiz, E., Sánchez, P. & Marticorena, A. 2018. Catálogo de the material was stained with 1% orcein solution. Metaphase chromo- las plantas vasculares de Chile. Gayana, Bot. 75: 1– 430. https:// some plates were photographed using a Zeiss Axioskop microscope, doi.org/10.4067/S0717-66432018000100001 with an incorporated video camera (Baeza & al., 2016). Solbrig, O. 1977. Chromosomal cytology and evolution in the family Compositae. Pp. 267–281 in: Heywood, V., Harborne, J. & LITERATURE CITED Turner, B. (eds.), The biology and chemistry of the Compositae, Al-Shehbaz, I.A. 2006. The genus Sisymbrium in South America, vol. 1. London: Academic Press. with synopses of the genera Chilocardamum, Mostacillastrum, Strother, J.L. & Panero, J.L. 1994. Chromosome studies: Latin Neuontobotrys, and Polypsecadium (Brassicaceae). Darwiniana American Compositae. Amer. J. Bot. 81: 770–775. https://doi. 44: 341–358. org/10.1002/j.1537-2197.1994.tb15513.x Baeza, C., Peñailillo, P., Novoa, P., Rosas, M., Finot, V.& Ruiz, E. Stuessy, T.F. & Sagastegui, A.A. 1993. Revision de Arnaldoa 2016. Chromosome reports from plants that grow in Chile. (Compositae, Barnadesioideae), género endémico del norte de IV. Gayana, Bot. 73: 183–191. https://doi.org/10.4067/S0717- Perú. Arnaldoa 1: 9–21. 66432016000200183 Stuessy, T.F., Sang, T. & DeVore, M. 1996. Phylogeny and biogeogra- Buitendijk, J.H. & Ramanna, M.S. 1996. Giemsa C-banded karyo- phy of the subfamily Barnadesioideae with implications for early types of eight species of Alstroemeria L. and some of their evolution of the Compositae. Pp. 463–490 in: Hind, D. & Beentje, hybrids. Ann. Bot. (Oxford) 78: 449–457. https://doi.org/10. H. (eds.), Compositae: Systematics; Proceedings of the International 1006/anbo.1996.0141 Compositae Conference, Kew, 1994, vol. 1. Richmond: Royal Bota- Cialdella, A. & López de Kiesling, A. 1981. Cariología de Schlechten- nic Gardens, Kew. dahlia luzulaefolia Less. (Compositae). Darwiniana 23: 357–360. Stuessy, T.F., Urtubey, E. & Gruenstaeudl, M. 2009. Barnadesioi- Cristóbal, C. 1986. El número cromosómico de dos Compositae– deae. Pp. 215–228 in: Funk, V.A., Susanna, A., Stuessy, Mutisieae. Bol. Soc. Argent. Bot. 24: 363–380. T.F. & Bayer, R.J. (eds.), Systematics, evolution, and biogeogra- Finot, V., Baeza, C., Muñoz-Schick, M., Ruiz, E., Espejo, J., Alar- phy of Compositae. Vienna: IAPT. cón, D., Carrasco, P., Novoa, P. & Eyzaguirre, M. 2018. Guía Urtubey, E. & Stuessy, T. 2001. New hypotheses of phylogenetic de campo de las Alstroemerias Chilenas. Concepción: Corma relationships in Barnadesioideae (Asteraceae) based on morphol- Editoriales. ogy. Taxon 50: 1043–1066. https://doi.org/10.2307/1224720 Grau, J. 1987. Chromosomenzahlen chilenischer Mutisieen (Com- Warwick, S.I., Sauder, C.A., Mayer, M.S. & Al-Shehbaz, I.A. positae). Bot. Jahrb. Syst. 108: 229–237. 2009. Phylogenetic relationships in the tribes Schizopetalae Gruenstaeudl, M., Urtubey, E., Jansen, R.K., Samuel, R., Bar- and Thelypoideae (Brassicaceae) based on nuclear ribosomal fuss, M.H.J. & Stuessy, T.F. 2009. Phylogeny of Barnadesioi- ITS region and plastid ndhF DNA sequences. Botany 87: deae (Asteraceae) inferred from DNA sequence data and 961–985. https://doi.org/10.1139/B09-051 morphology. Molec. Phylogen. Evol. 51: 572–587. https://doi. Watanabe, K., Yahara, T., Hashimoto, G., Nagatani, Y., Soejima, org/10.1016/j.ympev.2009.01.023 A., Kawahara, T. & Nakazawa, M. 2007. Chromosome num- Hunziker, J., Wulff, A., Xifreda, C. & Escobar, A. 1989. Estudios bers and karyotypes in Asteraceae. Ann. Missouri Bot. Gard. cariológicos en Compositae V. Darwiniana 29: 25–39. 94: 643–654. https://doi.org/10.3417/0026-6493(2007)94[643: Kiefer, M., Schmickl, R., German, D.A., Mandáková T., Lysak, CNAKIA]2.0.CO;2 M.A., Al-Shehbaz, I.A., Franzke, A., Mummenhoff, K., Sta- Wulff, A. 1984. Estudios cromosómicos en Compuestas de las floras matakis, A. & Koch, M.A. 2014. BrassiBase: Introduction to a Patagónica y Bonaerense. Darwiniana 25: 17–26. novel knowledge database on Brassicaceae evolution. Pl. Cell Wulff, A. 1990. Estudios cromosómicos en Barnadesiinae (Muti- Physiol. 55(1): e3. https://doi.org/10.1093/pcp/pct158 sieae, Asteraceae). I. Chuquiraga y Doniophyton. Darwiniana Koch, M.A., Kiefer, M., German, D.A., Al-Shehbaz, I.A., 30: 185–193. Franzke, A., Mummenhoff, K. & Schmickl, R. 2012. Brassi- Wulff, A. 1995. El cariotipo de Barnadesia odorata (Asteraceae). Base: Tools for biological resources to study characters and Hickenia 2: 129–130. traits in Brassicaceae – version 1.1. Taxon 61: 1001–1009. https://doi.org/10.1002/tax.615007 Marticorena, A. 2005. Malvaceae (excepto Cristaria). Pp. 22–105 IAPT chromosome data 30/2 in: Marticorena, C. & Rodríguez, R. (eds.), Flora de Chile, vol. 2(3). Concepción: Universidad de Concepción. Andrey S. Erst,* Elizaveta Yu. Mitrenina, Alexander P. Molina-Montenegro, M., Torres-Díaz, C., Carrasco-Urra, F., Sukhorukov, Igor V. Kuzmin, Polina D. Gudkova, González-Silvestre, L. & Gianoli, E. 2012. Plasticidad fenotí- Alexander N. Tashev, Kunli Xiang & Wei Wang pica en dos poblaciones antárticas de Colobanthus quitensis *Address for correspondence: [email protected] (Caryophyllaceae) bajo un escenario simulado de cambio global. Gayana, Bot. 69: 152–160. https://doi.org/10.4067/ The study was financially supported by the Russian Science S0717-66432012000100015 Foundation (Grant Nos. 18-34-20056 mol_a_ved and 18-04-00653

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A to Andrey Erst and Elizaveta Mitrenina [chromosome counts and BRASSICACEAE microphotographing] and Grant No. 18-34-20112 mol_а_ved to Thlaspi arvense L. Polina Gudkova [observations of the herbarium material in LE and 2n = 14, CHN. Russia, Tyumenskaya Oblast’, Tyumenskii field work in Tajikistan]), and the Scientific program (Grant Raion, 2 km NW of Uspenka, field margin, 5705′N, 6506′E, No. AAAA-A16-116021660045-2 to Alexander Sukhorukov [field 17 Sep 2017, I. Kuzmin 016 (NS). work in Africa]). LAMIACEAE * First chromosome count for the species. Lamium amplexicaule L. ** New cytotype for the species. 2n = 18, CHN. Israel, Jerusalem city, Giv’at Ram, near Hebrew University, 15 Mar 2017, A. Sukhorukov 001 (MW) [Fig. 2B]. Mitotic metaphase chromosomes were examined in root tips of seedlings. The method followed Smirnov (1968). Chromosome NYCTAGINACEAE numbers in literature were checked using CCDB v.1.45 (Rice *Boerhavia cordobensis Kuntze & al., 2015). 2n = 52, CHN. Namibia, Windhoek, 2234′15.3″N, 1705′07.4″E, weed in the city, 12 Mar 2017, A. Sukhorukov 002 (MW). ASPARAGACEAE Asparagus officinalis L. POACEAE 2n = 20, CHN. Russia, Novgorodskaya Oblast’, Veliky Novgo- Panicum miliaceum subsp. ruderale (Kitag.) Tzvelev rod, dry meadow on the left bank of the Volkhov River, 5834′N, 2n = 36, CHN. Russia, Tyumenskaya Oblast’, Tyumenskii 3117′E, 12 Oct 2017, I. Kuzmin 001 (NS). Raion, 2 km NW of Uspenka, field margin, 5705′N, 6506′E, 17 Sep 2017, I. Kuzmin 017 (NS). Polygonatum odoratum (Mill.) Druce 2n = 20, CHN. Russia, Tyumenskaya Oblast’, Tyumenskii Stipa glareosa P.A.Smirn. Raion, base recreation Lukashina, birch-pine forest, 5718′N, 2n = 44, CHN. Tajikistan, Gorno-Badakhshan Autonomous 6458′E, 19 Sep 2017, I. Kuzmin 246 (NS) [Fig. 2A]. Region, near Murghab, 3815′16.1″N, 7402′47.5″E, stony

Fig. 2. Mitotic metaphase chromosomes: A, Polygonatum odoratum, 2n = 20; B, Lamium amplexicaule, 2n = 18; C, Stipa glareosa,2n = 44; D, Eranthis hyemalis,2n = 16; E, Eranthis lobulata,2n = 16+0–1B; F, Eranthis sibirica,2n = 28; G, Eranthis sibirica,2n = 42; H, Hegemone lilacina,2n = 16; I, Trollius asiaticus,2n = 16. — Scale bars = 10 μm.

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slopes, 18 Aug 2016, P. Gudkova & M. Olonova 1 (TK); 3810′ Hegemone lilacina Bunge 55.7″N7357′59.0″E, P. Gudkova & M. Olonova 2 (TK); 3812′ 2n = 16, CHN. Russia, Altai Republic, Ulaganskii Raion, near 40.6″N, 7441′44.2″E, P. Gudkova & M. Olonova 3 (TK) Aktash village, 5020′24.6″N, 8744′48.0″E, 12 Jul 2018, A. Erst [Fig. 2C]. 433 [Fig. 2H] (NS).

RANUNCULACEAE Trollius altaicus C.A.Mey. Aquilegia canadensis L. 2n = 16, CHN. Russia, Altai Republic, Ulaganskii Raion, near 2n = 14, CHN. U.S.A., North Carolina, Charlotte, Forest, Ulagan village, 5041′52.7″N, 8759′44.2″E, 14 Jul 2018, A. Erst 06 Apr 2016, A.A Erst 018 (NS). 442 (NS).

Aquilegia ecalcarata Maxim. Trollius asiaticus L. 2n = 14, CHN. China, Gansu, Yongdeng, near Strim, 17 Jul 2n = 16, CHN. Russia, Novosibirskaya Oblast’, Novosibirsk, 2017, Y. Luo 1 (NS). Akademgorodok, Zyrianka River, 5449′20.7″N, 8306′18.4″E, 10 Jul 2017, A. Erst 457 [Fig. 2I] (NS). Aquilegia lactiflora Kar. & Kir. 2n = 14, CHN. Tajikistan, Rushon, Rovmed River gorge ROSACEAE (a tributary of Bartang River), the neighborhood of Kishlak Rovmed, Rosa rugosa Thunb. bank of the stream 3758′30.0″N, 7202′16.8″E, 17 Aug 2017, 2n = 14, CHN. Russia, Northwestern Federal District, Saint M. Olonova 13 (NS). Petersburg, west of Vasilevsky Island, Namiv, the coast of Gulf of Finland, 07 Oct 2017, I. Kuzmin 004 (NS). Aquilegia viridiflora Pall. 2n = 14, CHN. China, Gansu, Yongdeng, near rivulet, 16 Jul Rubus crataegifolius Bunge 2017, Y. Luo 2 (NS). 2n = 14, CHN. China, Beijing, Yun Xiu Gu Forest park, rocky ledges, 4036′16.6″N, 11724′28.0″E, 22 Jul 2016, A. Erst, T. Erst, Eranthis hyemalis (L.) Salisb. L. Lian, L. Bing & C. Shi 23 (NS). 2n = 16, CHN. Russia, Leningradskaya Oblast’, Saint Peters- burg, botanical garden, 09 Apr 2017, A. Grebenjuk 1 (NS) LITERATURE CITED [Fig. 2D]. Rice, A., Glick, L., Abadi, S., Einhorn, M., Kopelman, N.M., Salman-Minkov, A., Mayzel, J., Chay, O. & Mayrose, I. Eranthis lobulata W.T.Wang 2015. The chromosome counts database (CCDB) – A commu- *2n = 16+0–1B, CHN. China, Sichuan, Jiuding Shan Mountain, nity resource of plant chromosome numbers. New Phytol. 206: 14 May 2018, L. Zhang 1 (NS) [Fig. 2E]. 19–26. https://doi.org/10.1111/nph.13191 Smirnov, J.A. 1968. Uskorennyi metod issledovaniya somatiches- Eranthis longistipitata Regel kykh khromosom plodovykh [Accelerated method for studying *2n = 16, CHN. Kazakhstan, western part of the Kirghizsky somatic chromosomes in fruit trees]. Tsitologia 10: 1132– Ridge, Botamoynak Mountains, near Taraz City, 4254′26″N, 1134. [in Russian] 7132′09″E, 900 m, 24 Mar 2017, V. Kolbinzev 1 (NS).

Eranthis sibirica DC. **2n = 28, CHN. Russia, Khakassia Republic, Tashtypskii IAPT chromosome data 30/3 Raion, near Bol’shoj On village, left bank of the Bol’shoj On River, 5204′02.1″N, 8945′33.6″E, 04 May 2018, A. Erst & T. Erst 711; Kuljit Kaur,* Vijay Singh & Raghbir Chand Gupta Irkutskaya Oblast’, Shelehovskii Raion, Kuytun River, tributary *Address for correspondence: [email protected] of Irkut River, 3 km above Shamanka village, 5203′39.2″N, 10347′01.3″E, 492 m, 20 Jun 2019, A. Erst, D. Krivenko, E. Mitre- The study was supported by financial grant under IPLS Project nina & O. Chernysheva 12 [Fig. 2F] (NS). of DBT (BT/PR 4548/INF/22/146/2012), UGC-RGNF (F1-17.1/ **2n = 42, CHN. Russia, Irkutskaya Oblast’, Slyudiankskii 2011-12/RGNF-SC-PUN-1224) and UGC-BSR (Reference number: Raion, vicinity of Slyudyanka town, mixed forest, 5138′02.94″N, BT/PR 4548/INF/22/146/2012). 10341′13.90″E, 531 m, 02 May 2019, A. Erst, D. Krivenko & O. Chernysheva 13 [Fig. 2G]; Irkutskaya Oblast’, Slyudiankskii * New chromosome report (cytotype) for the species. Raion, Utulik River, 5132′50″N, 10402′45″E, 464 m, 20 Jun ** First chromosome count for an Indian accession. 2019, A. Erst, D. Krivenko, E. Mitrenina & O. Chernysheva 14; Irkutskaya Oblast’, Slyudiankskii Raion, Burovshina River, 5137′ BRASSICACEAE 06.00″N, 10349′16.17″E, 475 m, 20 Jun 2019, A. Erst, D. Krivenko, *Sisymbrium orientale L. E. Mitrenina & O. Chernysheva 15 (NS). n=18, CHN. India, Rajasthan, Sri Ganganagar, 2955′12″N, 7352′48″E, 165 m, along roadside, 04 Mar 2012, K. Kaur 28842 Eranthis stellata Maxim. (PUN 59768) [Fig. 3A]. 2n = 16, CHN. Russia, Primorskii Krai, Vladivostok City, Aka- Previously, for this species, only chromosome numbers 2n =16 demicheskaya Street, Mongolian Oak Forest, 4311′20.4″N, 13155′ and 28 were reported from India (Jeelani & al., 2013) and 2n =14 25.4″E, 12 Apr 2018, V. Yakubov 12 (NS). only from outside of India (Strid & Franzén, 1981).

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CACTACEAE CAMPANULACEAE *Opuntia elatior Mill. **Campanula latifolia L. n = 11, CHN. India, Rajasthan, Churu, Chappar, 2749′12″N, n=14, CHN. India, Himachal Pradesh, Sirmaur, Haripurdhar, 7424′02″E, 302 m, along roadside on dry land, K. Kaur 32810 3034′48″N, 7733′01″E, 2400 m, on grassy slopes, 17 Jun 2012, (PUN 60371) [Fig. 3B]. V. Singh 33591 (PUN 60123) [Fig. 3C]. Previously, tetraploid (2n = 44) and hexaploid (2n = 66) cyto- This report agrees with the previously published reports of types were reported for this species from India (Roy & Mishra, 2n = 34 for the species from outside of India (Lövkvist & Hult- 1961; Sanjappa, 1979). gård, 1999).

Fig. 3. A Sisymbrium orientale, PMC at anaphase I, n=18 (PUN 59768); B, Opuntia elatior, PMC at metaphase I, n=11 (PUN 60371); C, Campanula latifolia, PMC at metaphase I, n=14 (PUN 60123); D, Cyananthus lobatus, PMC at diakinesis, n=14 (PUN 58810); E, Lonicera hispida, PMC at metaphase I, n=9 (PUN 60131); F, Morina longifolia, PMC at diakinesis, n=17 (PUN 60103); G, Arenaria serpyllifolia, PMC at metaphase I, n=9 (PUN 59766); H, Indigofera caerulea, PMC at metaphase I, n=8 (PUN 60418); I, Tephrosia candida, PMC at metaphase I, n=22 (PUN 60377); J, Jaeschkea gentianoides, PMC at anaphase I, n= 10 (PUN 60092); K, Lycopus europaeus, PMC at metaphase I, n=11+1B (arrow) (PUN 60058); L, Syringa emodii, PMC at metaphase I, n=22 (PUN 60030); M, Polygala erioptera, PMC at metaphase I, n=24 (PUN 59693); N, Veronica persica, PMC at metaphase I, n=16+1B (arrow) (PUN 60094); O, Verbena officinalis, PMC at diakinesis, n= 28 (PUN 58664). — Scale bars = 10 μm.

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**Cyananthus lobatus Wall. ex Benth. The present report agrees with the previous one (2n = 22) n=14, CHN. India, Himachal Pradesh, Sirmaur, Churdhar, reported from outside of India (Lövkvist & Hultgård, 1999). 3052′34″N, 7724′04″E, 3650 m, on grassy slopes, 01 Sep 2013, V. Singh 31303 (PUN 58810) [Fig. 3D]. OLEACEAE Present report agrees with the earlier one (2n = 14) from outside *Syringa emodi Wall. ex G.Don of India (Eddie & al., 2003). n=22, CHN. India, Himachal Pradesh, Sirmaur, Tisri, 3051′ 34″N, 7727′23″E, 3100 m, on rocky places, 17 Aug 2014, V. Singh CAPRIFOLIACEAE 33598 (PUN 60030) [Fig. 3L]. Lonicera hispida Pall. ex Schult. Earlier, only 2n = 46 was reported for this species from India n=9, CHN. India, Himachal Pradesh, Sirmaur, Churdhar, (Mehra, 1976). 3052′34″N, 7724′04″E, 3650 m, in rocky places, 09 Aug 2013, V. Singh 33599 (PUN 60131) [Fig. 3E]. POLYGALACEAE The present report agrees with the previous report of 2n =18 *Polygala erioptera DC. from India (Sandhu & Mann, 1989). n=24, CHN. India, Rajasthan, Udaipur, Saheliyon Ki Bari, 2436′10″N, 7341′07″E, 598 m, on moist shady place, 12 Sep **Morina longifolia Wall. ex DC. 2013, K. Kaur 31270 (PUN 59693) [Fig. 3M]. n=17, CHN. India, Himachal Pradesh, Sirmaur, Tisri, 3051′ Previously, 2n = 48 was reported from outside of India 34″N, 7727′23″E, 3100 m, along roadside, 16 Aug 2014, V. Singh (Khatoon & Ali, 1993), and 2n = 76 was reported from India 33571 (PUN 60103) [Fig. 3F]. (Sharma & Mehra, 1978). This reports agrees with the previous one (2n = 34) from outside of India (Verlaque, 1977). SCROPHULARIACEAE *Veronica persica Poir. CARYOPHYLLACEAE n=16+0–1B, CHN. India, Himachal Pradesh, Sirmaur, Tisri, *Arenaria serpyllifolia L. 3051′34″N, 7727′23″E, 3100 m, on moist places, 17 Aug 2014, n=9, CHN. India, Rajasthan, Sri Ganganagar, 2955′12″N, V. Singh 33562 (PUN 60094) [Fig. 3N]. 7352′48″E, 165 m, in Brassica field, moist land, 05 Mar 2012, Previously, 2n = 14 and 28 were reported from India K. Kaur 28840 (PUN 59766) [Fig. 3G]. (Vasudevan, 1975; Bala & Gupta, 2011), while only 2n =28 Previously, for this species, chromosome numbers 2n = 22 and was reported from outside of India (Mehrvarz & Kharabian, 30 (Kliphuis & Weiffering, 1979; Nazarova, 2004) were reported 2005). B-chromosomes are reported here for the first time for this from outside of India, while 2n = 20 was reported from India as well species. as outside of this country (Jeelani & al., 2011). VERBENACEAE FABACEAE Verbena officinalis L. Indigofera caerulea Roxb. n=28, CHN. India, Himachal Pradesh, Sirmaur, Sangrah, n=8, CHN. India, Rajasthan, Jhalawar, Gagron fort, 2437′ 3034′48″N, 7713′23″E, 1400 m, along roadside, 09 Jul 2013, 40″N, 7610′58″E, 290 m, on moist place, 23 Aug 2014, K. Kaur V. Singh 30457 (PUN 58664) [Fig. 3O]. 32859 (PUN 60418) [Fig. 3H]. An octoploid chromosome count 2n = 56 was already reported This report agrees with the previous one from India from India (Bir & Saggoo, 1979). (Sanjappa, 1983). LITERATURE CITED *Tephrosia candida DC. Bala, S. & Gupta, R.C. 2011. Cytological investigations on some n=22, CHN. India, Rajasthan, Churu, 2818′10″N, 7457′ north Indian Bicarpellatae. Cytologia 76: 261–267. https://doi. 07″E, 290 m, on dry land, 20 Oct 2013, K. Kaur 32817 (PUN org/10.1508/cytologia.76.261 60377) [Fig. 3I]. Bir, S.S. & Saggoo, M.I.S. 1979. [Reports]. In: Löve, Á. (ed.), IOPB Earlier, this species was only known to have 2n = 22 from India chromosome number reports LXV. Taxon 28: 630–631. https:// (Kumari & Bir, 1990) and 2n = 24 from outside of India (Gill doi.org/10.1002/j.1996-8175.1979.tb00530.x & Husaini, 1986). Eddie, W.M., Shulkina, T., Gaskin, J., Haberle, R.C. & Jansen, R.K. 2003. Phylogeny of Campanulaceae s.str. inferred from GENTIANACEAE ITS sequences of nuclear ribosomal DNA. Ann. Missouri Bot. *Jaeschkea gentianoides Kurz Gard. 90: 334–375. https://doi.org/10.2307/3298542 n=10, CHN. India, Himachal Pradesh, Sirmaur, Churdhar, Gill, L.S. & Husaini, S.W. 1986. Cytological observations 3052′34″N, 7724′04″E, 3650 m, on moist, shady place, 16 Aug in Leguminosae from southern Nigeria. Willdenowia 15: 2014, V. Singh 33560 (PUN 60092) [Fig. 3J]. 521–527. Previously, two cytotypes were reported for this species from India, Jeelani, S.M., Rani, S., Kumar, S., Kumari, S. & Gupta, R.C. i.e., 2n = 18 (Koul & Gohil, 1973) and 2n = 22 (Raina & Ashruf, 1981). 2011. Meiotic studies in some members of Caryophyllaceae Juss. from the western Himalayas. Acta Biol. Cracov., Ser. Bot. LAMIACEAE 53: 86–95. https://doi.org/10.2478/v10182-011-0012-5 **Lycopus europaeus L. Jeelani, S.M., Rani, S., Kumar, S., Kumari, S. & Gupta, R.C. n = 11+1B, CHN. India, Himachal Pradesh, Sirmaur, Chapdhar, 2013. Cytological studies of Brassicaceae Burn. (Cruciferae 3049′07″N, 7725′06″E, 2200 m, shady places, 16 Aug 2014, Juss.) from western Himalayas. Cytol. & Genet. 47: 20–28. V. Singh 33523 (PUN 60058) [Fig. 3K]. https://doi.org/10.3103/S0095452713010076

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Khatoon, S. & Ali, S.I. 1993. Chromosome atlas of the angiosperms of ASTERACEAE (COMPOSITAE) Pakistan. Karachi: Department of Botany, University of Karachi. Achillea asiatica Serg. Kliphuis, E. & Wieffering, J.H. 1979. [Report]. In: Löve, A. (ed.), 2n = 18, CHN. Russia, Republic of Sakha (Yakutia), Aldanskii IOPB chromosome numbers reports LXIV. Taxon 28: 398–400. Raion, 35 km along the A-360 highway to NNE from Bolshoi Kha- https://doi.org/10.1002/j.1996-8175.1979.tb00530.x tymi settlement, 1145 m, 5739′45.8″ N, 12513′05.1″ E, roadside, Koul, A.K. & Gohil, R.N. 1973. Cytotaxonomical conspectus of the 17 Aug 2017, D.A. Krivenko 2018-34 (LE). flora of Kashmir (1), Chromosome numbers of some common plants. Phyton (Horn) 15: 57–66. Achillea micrantha Willd. Kumari, S. & Bir, S.S. 1990. Karyomorphological evolution in 2n = 18, CHN. Russia, Astrakhanskaya Oblast’, Narimanovskii Papilionaceae. J. Cytol. Genet. 25: 173–219. Raion, Ilmeni (system of freshwater and saltwater lakes in the west- Lövkvist, B. & Hultgård, U.M. 1999. Chromosome numbers in ern part of the delta of the Volga River), 29 Aug 2017, E.M. Machs south Swedish vascular plants. Opera Bot. 137: 1–42. 2018-04 (LE); Russia, Astrakhanskaya Oblast’, Narimanovskii Mehra, P.N. 1976. Cytology of Himalayan hardwoods. Calcutta: Raion, Lineinoe settlement, 01 Sep 2017, E.M. Machs 2018-02 SreeSaraswaty. (LE), E.M. Machs 2018-03 (LE); Russia, Republic of Kalmykia, Mehrvarz, S.S. & Kharabian, A. 2005. Chromosome counts of Yashkulskii Raion, Hulhuta settlement, sandy mounds of Baer, some Veronica L. (Scrophulariaceae) species from Iran. Turkish 04 Sep 2017, E.M. Machs 2018-01 (LE). J. Bot. 29: 263–267. Nazarova, E. 2004. The karyological investigation of the vetch Achillea millefolium L. (Vicia L., Fabaceae) from Armenia. Fl. Rastitel’n. Rastitel’n. 2n = 36, CHN. Russia, Leningradskaya Oblast’, Vsevolozhskii Resursy Armenii 15: 95–97. Raion, Lembolovo railway station, gravelly place at base of mound, Raina, R. & Ashruf, M. 1981. [Report]. In: Löve, A. (ed.), Chromo- 10 Aug 2017, V.I. Dorofeev 2017-08 (LE); Russia, Republic of Sakha some number reports LXXII. Taxon 30: 707. https://doi.org/10. (Yakutia), outskirts of Neryungri town, 865 m, 5639′22.69″N, 1002/j.1996-8175.1981.tb04287.x 12442′09.39″E, edge of the road, 19 Aug 2017, D.A. Krivenko Roy, R.P & Mishra, N.C. 1961. Cytological studies in Cactaceae. 2018-39 (LE). Proc. Indian Sci. Congr. Assoc. 48(3): 300–301. Sandhu, P.S. & Mann, S.K. 1989. SOCGI plant chromosome num- Antennaria dioica (L.) Gaertn. ber reports – VIII. J. Cytol. Genet. 24: 179–183. 2n = 18, CHN. Russia, Republic of Sakha (Yakutia), Aldanskii Sanjappa, M. 1979. [Report]. In: Löve, A. (ed.), IOPB chromosome Raion, about 19 km S of Bolshoi Nimnyr settlement, 1167 m, number reports. LXIV. Taxon 28: 393–395. https://doi.org/10. 5751′47″N, 12531′30″E, lichen shrub birch-larch forest, 23 Aug 1002/j.1996-8175.1979.tb00530.x 2017, D.A. Krivenko 2018-58 (LE). Sanjappa, M. 1983. [Report]. In: Löve, A. (ed.), IOPB chromosome number reports LXXXI. Taxon 32: 668. https://doi.org/10.1002/ Artemisia absinthium L. j.1996-8175.1983.tb03658.x 2n = 18, CHN. Russia, Kaliningradskaya Oblast’, Zelenograds- Sharma, M.L. & Mehra, P.N. 1978. Chromosome numbers in some kii Raion, Curonian Spit, in Lesnoi village, near the lighthouse, north-west Indian species of Polygala. Cytologia 43: 589–593. 10 Nov 2017, G.Yu. Konechnaya 2018-22 (LE); Russia, Leningrads- https://doi.org/10.1508/cytologia.43.589 kaya Oblast’, Tosnenskii Raion, Tosno II railway station, railway Strid, A. & Franzén, R. 1981. [Report]. In Löve, A. (ed.), Chromo- mound, 10 Dec 2017, G.Yu. Konechnaya 2018-28 (LE). some number reports LXXIII. Taxon 30: 829–842. https://doi. org/10.1002/j.1996-8175.1981.tb04309.x Artemisia arenaria DC. Vasudevan, K.N. 1975. Contribution to the cytotaxonomy and cyto- 2n = 36, CHN. Russia, Kaliningradskaya Oblast’, Zelenograds- geography of the flora of the western Himalayas (with an attempt kii Raion, Curonian Spit, sandy terrace near bird traps, 03 Nov 2017, to compare it with the flora of the Alps). Part II. Ber. Schweiz. G.Yu. Konechnaya 2018-20 (LE); Russia, Republic of Kalmykia, Bot. Ges. 85: 210–252. vicinity of Elista city, 10 Nov 2017, R.R. Dzhapova 2018-07 (LE), Verlaque, R. 1977. [Report]. In: Löve, A. (ed.), IOPB chromosome R.R. Dzhapova 2018-08 (LE). number reports LVI. Taxon 26: 257–27. https://doi.org/10.1002/ j.1996-8175.1977.tb04167.x Artemisia austriaca Jacq. 2n = 18, CHN. Russia, Republic of Kalmykia, vicinity of Elista city, 10 Nov 2017, R.R. Dzhapova 2018-11 (LE), R.R. Dzhapova IAPT chromosome data 30/4 2018-13 (LE).

Aleksandr A. Korobkov, Violetta V. Kotseruba & Artemisia borealis Pall. Denis A. Krivenko* 2n = 18, CHN. Russia, Tyumenskaya Oblast’, Yamalo- *Address for correspondence: [email protected] Nenetskii Avtonomnyi Okrug, Priuralskii Raion, lower part of Nya- ravoihadata River, 50 m, pebbled river floodplain, 13 Aug 2017, We are grateful to the scientists of the Astrakhan Nature Reserve N.N. Pospelov 2018-06 (LE). K.V. Litvinov and N.O. Meshcheryakova as well as scientist of the Chornye Zemli Nature Reserve V.E. Badmaev and Professor of Artemisia campestris L. Kalmyk State University R.R. Dzhapova for help in collecting 2n = 36, CHN. Russia, Leningradskaya Oblast’, Tosnenskii material. The study of Asteraceae was funded by RFBR, Raion, Tosno II railway station, railway mound, 10 Dec 2017, no. 16-04-00052, and the study of Ranunculaceae was funded G.Yu. Konechnaya 2018-25 (LE), G.Yu. Konechnaya 2018-26 by RSF, no. 19-74-10082. (LE); Russia, Kaliningradskaya Oblast’, Zelenogradskii Raion,

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Curonian Spit, sandy terrace near bird traps, 03 Nov 2017, G.Yu. 2n = 36, CHN. Russia, Republic of Kalmykia, vicinity of Elista Konechnaya 2018-19 (LE), G.Yu. Konechnaya 2018-21 (LE); city, 10 Nov 2017, R.R. Dzhapova 2018-10b (LE). Russia, Saint Petersburg city, Vyborgskii Raion, sand terrace of Olginskii pond, mixed herb-sagebrush group, 08 Nov 2017, A.A. Artemisia lithophila Turcz. ex DC. Korobkov s.n. (LE); Russia, Kaliningradskaya Oblast’, Zeleno- 2n = 18, CHN. Russia, Republic of Buryatia, Dzhidinskii Raion, gradskii Raion, Curonian Spit, in Lesnoi village, near the light- foot of southern macroslope of Malyi Hamar-Daban Mt. range, Bai- house, 10 Nov 2017, G.Yu. Konechnaya 2018-23 (LE), G.Yu. kal Nature Reserve, left bank of Temnik River below the confluence Konechnaya 2018-24 (LE). of Verkhnyaya Khandagaita River, 1120 m, steep slope, southern exposure, stones, 20 Aug 2017, N.S. Gamova 2018-64 (LE). Artemisia commutata Besser 2n = 18, CHN. Russia, Republic of Sakha (Yakutia), Yakutsk Artemisia mongolica (Fisch. ex Besser) Nakai city, near the airport, 118 m, 6205′10.44″N 12944′34.47″E, 2n = 16, CHN. Russia, Republic of Sakha (Yakutia), Aldanskii mixed herb-sagebrush groups, 26 Aug 2017, D.A. Krivenko Raion, 35 km along the A-360 highway to NNE from Bolshoi Kha- 2018-29 (LE). tymi settlement, 1145 m, 5739′45.8″ N, 12513′05.1″ E, roadside, 2n = 36, CHN. Russia, Republic of Buryatia, Dzhidinskii 17 Aug 2017, D.A. Krivenko 2018-37 (LE). Raion, southern macroslope of Malyi Hamar-Daban Mt. range, Samhak cordon of Baikal Nature Reserve, steppefied meadow, Artemisia pauciflora Weber ex Stechm. 16 Aug 2017, N.S. Gamova 2018-65 (LE); Russia, Republic of 2n = 18, CHN. Russia, Republic of Kalmykia, vicinity of Elista Buryatia, Tunkinskii Raion, near Mondy village, left bank of Irkut city, 10 Nov 2017, R.R. Dzhapova 2018-12 (LE). River, 5140′15″N, 10102′10″E, riverbank, 14 Sep 2017, D.A. Krivenko 2018-42 (LE); Russia, Republic of Bashkortostan, Davle- Artemisia phaeolepis Krasch. kanovskii Raion, 0.8 km west of Yangi-Turmush village, south 2n = 36, CHN. Russia, Republic of Buryatia, Tunkinskii Raion, shore of Aslikyul lake, rocky steppe, 20 Sep 2017, A.A. Muldeshev near Mondy village, left bank of Irkut River, 5140′15″N, 10102′ 2018-63 (LE). 10″E, riverbank, 14 Sep 2017, D.A. Krivenko 2018-45 (LE), D.A. Krivenko 2018-46 (LE). Artemisia desertorum Spreng. 2n = 18, CHN. Russia, Republic of Sakha (Yakutia), Yakutsk Artemisia santonicum L. city, near the airport, 118 m, 6205′10.44″N, 12944′34.47″E, mixed 2n = 36, CHN. Russia, Republic of Kalmykia, vicinity of Elista herb-sagebrush groups, 26 Aug 2017, D.A. Krivenko 2018-31 (LE); city, 10 Nov 2017, R.R. Dzhapova 2018-09 (LE). Russia, Zabaikalskii Krai, Borzinskii Raion, near the south coast of Toreiskie lakes, mixed herb-grass steppe, 08 Sep 2015, T.E. Tkachuk Artemisia sieversiana Ehrh. ex Willd. 2017-11 (LE), T.E. Tkachuk 2017-12 (LE), T.E. Tkachuk 2017-13 2n = 18, CHN. Russia, Irkutskaya Oblast’, Irkutsk city, left bank (LE). of Angara River, Akademgorodok, 5214′55″N, 10416′26″E, ruder- alized plant groups, 19 Sep 2017, D.A. Krivenko 2018-49 (LE). Artemisia dolosa Krasch. 2n = 36, CHN. Russia, Russia, Republic of Buryatia, Tunkinskii Artemisia vulgaris L. Raion, near Mondy village, left bank of Irkut River, 5140′15″N, 2n = 16, CHN. Russia, Leningradskaya Oblast’, Tosnenskii 10102′10″E, riverbank, 14 Sep 2017, D.A. Krivenko 2018-43 Raion, Tosno II railway station, railway mound, 10 Dec 2017, (LE), D.A. Krivenko 2018-44 (LE). G.Yu. Konechnaya 2018-27 (LE).

Artemisia dracunculus L. Aster alpinus L. 2n = 18, CHN. Russia, Republic of Sakha (Yakutia), Yakutsk 2n = 18, CHN. Russia, Republic of Buryatia, Tunkinskii Raion, city, near the airport, 118 m, 6205′10.44″N, 12944′34.47″E, Mondy village, right bank of Irkut River, 5140′07″N, 10059′03″E, mixed herb-sagebrush groups, 26 Aug 2017, D.A. Krivenko mixed herb-grass slope, 14 Sep 2017, D.A. Krivenko 2018-51 (LE). 2018-30 (LE). Aster sibiricus L. Artemisia frigida Willd. 2n = 18, CHN. Russia, Republic of Sakha (Yakutia), Neryun- 2n = 36, CHN. Russia, Republic of Buryatia, Tunkinskii Raion, grinskii Raion, about 42 km SE of Chulman urban-type settlement, near Mondy village, left bank of Irkut River, 5140′15″N, 10102′ right bank of Khatma River, 881 m, 5710′11″N, 12515′41″E, 10″E, riverbank, 14 Sep 2017, D.A. Krivenko 2018-47 (LE), D.A. sedge-smallweed-iris floodplain meadow, 11 Aug 2017, D.A. Kri- Krivenko 2018-48 (LE). venko 2018-60 (LE).

Artemisia jacutica Drobow Chamomilla suaveolens (Pursh) Rydb. 2n = 18, CHN. Russia, Republic of Sakha (Yakutia), Yakutsk 2n = 18, CHN. Russia, Republic of Sakha (Yakutia), Neryun- city, near the airport, 118 m, 6205′10.44″N, 12944′34.47″E, grinskii Raion, about 32 km SE of Bolshoi Khatymi, left bank of mixed herb-sagebrush groups, 26 Aug 2017, D.A. Krivenko Tit River, 981 m, 5729′21″N, 12525′15″E, sandy riverbank, mixed 2018-32 (LE). herb-grass,19 Aug 2017, D.A. Krivenko 2018-56 (LE).

Artemisia lerchiana Stechm. Dendranthema zawadskii (Herbich) Tzvelev 2n = 18, CHN. Russia, Republic of Kalmykia, vicinity of Elista 2n = 72, CHN. Russia, Republic of Buryatia, Tunkinskii city, 10 Nov 2017, R.R. Dzhapova 2018-10a (LE). Raion, Mondy village, right bank of Irkut River, 5140′07″N,

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10059′03″E, mixed herb-grass slope, 14 Sep 2017, D.A. Krivenko IAPT chromosome data 30/5 2018-50 (LE). Viktor O. Nachychko Heteropappus altaicus (Willd.) Novopokr. Address for correspondence: [email protected] 2n = 18, CHN. Russia, Republic of Sakha (Yakutia), Yakutsk city, near the airport, 118 m, 6205′10.44″N, 12944′34.47″E, mixed The study was financially supported by research grants from herb-sagebrush groups, 26 Aug 2017, D.A. Krivenko 2018-54 (LE); Ivan Franko National University of Lviv, Ukraine for the support of Russia, Republic of Buryatia, Tunkinskii Raion, near Mondy village, fundamental research at Lviv University (projects PNS-010515, left bank of Irkut River, 5140′15″N, 10102′10″E, riverbank, 14 Sep PN-010115). 2017, D.A. Krivenko 2018-52 (LE). * First chromosome count for the species. Ptarmica alpina (L.) DC. ** First chromosome count from Ukraine. 2n = 36, CHN. Russia, Republic of Sakha (Yakutia), outskirts of Neryungri town, 865 m, 5639′22.69″N, 12442′09.39″E, roadside, LAMIACEAE 19 Aug 2017, D.A. Krivenko 2018-41 (LE); Russia, Republic of Salvia glutinosa L. Sakha (Yakutia), Aldanskii Raion, 14.5 km along the A-360 high- n =8,2n = 16, CHN. Ukraine, Lviv Region, Lviv, woodland park way to S from Bolshoi Nimnyr settlement, 1150 m, 5754′48.5″N, “Pohulyanka”, among bushes, 4949′45.4″N, 2404′00.1″E, 318 m, 12530′37.9″E, roadside, 20 Aug 2017, D.A. Krivenko 2018-33 21 Aug 2019, V.O. Nachychko VN 1428 (CWU, KW, LWKS) [Fig. 4A,B]. (LE). Thymus alpestris Tausch ex A.Kern. Tanacetum boreale Fisch. ex DC. 2n = 28, CHN. Ukraine, Transcarpathian Region, Rakhiv Dis- 2n = 18, CHN. Russia, Republic of Sakha (Yakutia), Aldanskii trict, Kvasy village, Mt. Petros, southern macroslope, among grasses, Raion, 40 km along the A-360 highway to S from Bolshoi Nimnyr 4810′19.7″N, 2425′21.8″E, 1987 m, 27 Jul 2018, V.O. Nachychko settlement, 1160 m, 5742′01″N, 12516′50″E, crude roadside, VN 1374 (CWU, KW, LWKS) [Fig. 4C]. 08 Aug 2017, D.A. Krivenko 2018-36 (LE); Russia, Republic of Sakha (Yakutia), Aldanskii Raion, 35 km along the A-360 highway **Thymus alternans Klokov to NNE from Bolshoi Khatymi settlement, 1145 m, 5739′45.8″N, 2n = 56, CHN. Ukraine, Transcarpathian Region, Rakhiv Dis- 12513′05.1″E, roadside, 17 Aug 2017, D.A. Krivenko 2018-35 trict, Kvasy village, meadow on the side of the highway, among (LE). grasses, 4810′27.3″N, 2417′05.9″E, 572 m, 13 Jul 2017, V.O. Nachychko VN 1271 (CWU, KW, LWKS) [Fig. 4D]. Tripleurospermum inodorum (L.) Sch.Bip. The presented chromosome number report is the first from 2n = 18, CHN. Russia, Astrakhanskaya Oblast’, Narimanovskii Ukraine and the second one for the species. It corresponds with the Raion, Ilmeni (system of freshwater and saltwater lakes in the west- previous report (Mártonfi & Mártonfiová, 1996) made on material ern part of the delta of the Volga River), 29 Aug 2017, E.M. Machs from the Slovak Republic, and confirms 2n = 56 for this species cur- 2018-05 (LE). rently treated as East-South-Apuseni-Carpathian subendemic 2n = 36, CHN. Russia, Republic of Sakha (Yakutia), outskirts of (Kliment & al., 2016). Neryungri town, 865 m, 5639′22.69″N, 12442′09.39″E, roadside, 13 Aug 2017, D.A. Krivenko 2018-40 (LE). *Thymus moldavicus Klokov & Des.-Shost. 2n = 28, CHN. Ukraine, Ivano-Frankivsk Region, Tlumach Dis- RANUNCULACEAE trict, Zhabokruky village, south-western outskirts, on a gypsum out- Anemone nemorosa L. crop, 4844′38.0″N, 2509′16.8″E, 284 m, 20 Jul 2015, V.O. 2n = 30, CHN. Bulgaria, Montana Province, Berkovitsa Munic- Nachychko VN 1038 (KW, LWKS, WU) [Fig. 4E]. ipality, Balkan Mt. range, Petrohan Pass, Petrohan, 4307′17.1″N, This is the first chromosome number report for this species, 2307′29.8″E, 1354 m, 10 May 2019, A.S. Erst & A. Tashev 02 which is currently treated as South-Pontian and West-Podolian (NS). endemic (Nachychko & Honcharenko, 2017).

Anemone ranunculoides L. METHODS 2n = 32, CHN. Bulgaria, Bulgaria, Sofia Province, Kostinbrod Mitotic chromosome numbers were determined from the root Municipality, Ponor, 4254′42.3″N, 2309′46.8″E, 859 m, 10 May tips of plants replanted from the field and cultivated in the Botanical 2019, A.S. Erst & A. Tashev 01 (NS). Garden of Ivan Franko National University of Lviv, Ukraine. The laboratory protocol used was described in Nachychko & al. (2019). For Eranthis stellata Maxim. determination of the meiotic chromosome number were used pollen- 2n = 16, CHN. Russia, Primorskii Krai, Vladivostok city, mother cells obtained from the anthers of fresh flower buds, and fixed Malaya Sedanka River, 4312′36″N, 13159′24″E, 16 Apr 2019, and stained in the drop of 2% aceto-orcein on the slide for microscopy. V.Yu. Nikulin & A.Yu. Nikulin 01 (NS). LITERATURE CITED Trollius altaicus C.A.Mey. Kliment, J., Turis, P. & Janišová, M. 2016. Taxa of vascular plants 2n = 16, CHN. Russia, Republic of Altai, Ulaganskii Raion, endemic to the Carpathian Mts. Preslia 88: 19–76. Aktashskii Radio repeater, 3054 m, 5020′24.0″N, 8744′55.2″E, Mártonfi, P. & Mártonfiová, L. 1996. Thymus chromosome num- 10 Aug 2018, A.S. Erst & T.V. Erst 128 (NS). bers from Carpathians and Pannonia. Thaiszia 6: 25–38.

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Fig. 4. A, Salvia glutinosa, meiotic anaphase I, n =8;B, S. glutinosa, mitotic metaphase, 2n = 16; C, Thymus alpestris, mitotic metaphase, 2n = 28; D, T. alternans, mitotic metaphase, 2n = 56; E, T. moldavicus, mitotic metaphase, 2n = 28. — Scale bars = 5 μm.

Nachychko, V. & Honcharenko, V. 2017. Botaniko-heohrafichna ACTINIDIACEAE kharakterystyka vydiv rodu Thymus L. (Lamiaceae) u flori Actinidia polygama (Siebold & Zucc.) Maxim. zakhidnykh rehioniv Ukrayiny [Botanical-geographical char- 2n = 58; CHN. Russia, Far East, Primorskii Krai, Muravëv- acterization of Thymus L. (Lamiaceae) species in the flora of Amurskii Peninsula [near Vladivostok], right riverside of the Levaya western regions of Ukraine]. Visn. Lviv Univ. Ser. Biol. 75: Sedanka (Pionerskaya) River, the upper course, Bogataya Griva Ridge, 35–47. [in Ukrainian] http://doi.org/10.30970/vlubs.2017. coniferous-broadleaved forest (Abies holophylla, Pinus koraiensis and 75.04 broadleaved species), 25 Oct 2018, E.B. Volynets 13350 (VLA). Nachychko, V.O.,Sosnovsky, Y.V.& Honcharenko, V.I. 2019. First record of Balkan Thymus jankae (Lamiaceae) from Ukraine, ASTERACEAE with taxonomic remarks on the species. Bot. Lett. 166: 41–50. Artemisia stelleriana Besser. https://doi.org/10.1080/23818107.2018.1544507 2n = 18; CHN. Russia, Far East, Kamchatka Peninsula, near the mouth of the Nalychevo River, sandy beach, 05 Sep 2018, O.A. Cher- nyagina 13410 (VLA). IAPT chromosome data 30/6 *Bidens kamtschatica Vassilcz. Nina S. Probatova* & Olga A. Chernyagina 2n = 48; CHN. Russia, Far East, Kamchatka Peninsula, the Gey- *Address for correspondence: [email protected] ser Valley, 2018, O.A. Chernyagina 13349 (VLA).

The study was supported by the Federal Agency for Scientific Bidens parviflora Willd. Organizations program for support the bioresource collections. 2n = 24; CHN. Russia, Far East, Primorskii Krai, Vladivostok city, as a weed on flowerbed by Ovchinnikova Str. 30, 17 Oct 2018, * First chromosome count for the species. N.S. Probatova & G.G. Probatova 13298 (VLA).

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*Leontopodium kamtschaticum Kom. PAPAVERACEAE 2n = 26; CHN. Russia, Far East, Kamchatka Peninsula, Ush- Chelidonium asiaticum (Hara) Krahulc. kovskii, 2018, Korablëv 13335 (VLA). 2n = 10; CHN. Russia, Far East, Primorskii Krai, Vladivostok city, Pervorechenskii District, on the way to Postysheva bus stop, on Picris kamtschatica Ledeb. lower part of the slope, 15 Nov 2018, G.G. Probatova 13301 (VLA). 2n = 10; CHN. Russia, Far East, Kamchatka Peninsula, Ust’- Bol’sheretskii Raion, at the Apachinskie hot springs, 29 Sep 2018, POACEAE O.A. Chernyagina 13323 (VLA). Agrostis clavata Trin. 2n = 42; CHN. Russia, Far East, Kamchatka Peninsula, Naly- Solidago canadensis L. chevo Lake, 2018, O.A. Chernyagina 13395 (VLA). 2n = 18; CHN. Sweden, in vicinity of Katrinholm town, near Marmorbyn village, on the margin of Picea forest, 02 Nov 2018, Agrostis geminata Trin. O.A. Chernyagina 13328 (VLA). 2n = 28; CHN. Russia, Far East, Kamchatka Peninsula, Elizovs- kii Raion, foothills of Mutnovskii Volcano, near Dachnye hot Solidago virgaurea L. springs, Aktivnye group, thermal plots, 26 Aug 2018, O.A. Chernya- 2n = 18; CHN. Sweden, in vicinity of Katrinholm town, near gina 13402 (VLA). Marmorbyn village, on the margin of Picea forest, 02 Nov 2018, O.A. Chernyagina 13336 (VLA). Arrhenatherum elatius (L.) P.Beauv. ex J.Presl & C.Presl 2n = 28; CHN. Sweden, vicinity of Katrinholm town, near Mar- *Taraxacum natschikense Kom., morbyn village, on riverside, 03 Nov 2018, O.A. Chernyagina 13403 2n = 24; CHN. Russia, Far East, Kamchatka Peninsula, Elizovs- (VLA). kii Raion, foothills of Mutnovskyi Volcano, near Dachnye hot springs, nival meadows, 26 Aug 2018, O.A. Chernyagina 13326 Beckmannia syzigachne (Steud.) Fernald (VLA). 2n = 14; CHN. Russia, Far East, Kamchatka Peninsula, Bystrinskii Raion, near Anavgai village, floodplain of the Anavgai BRASSICACEAE River, puddle on the road, 29 Aug 2018, O.A. Chernyagina 13385 Draba hirta L. (VLA). 2n = 64; CHN. Russia, Far East, Kamchatka Peninsula, outskirts of Petropavlovsk-Kamchatskii city, 2018, O.A. Chernyagina Elymus pendulinus (Nevski) Tzvelev 13346 (VLA). 2n = 28; CHN. Russia, Far East, Primorskii Krai, Muravëv- Amurskii Peninsula, near Akademgorodok [Vladivostok city], forest CARYOPHYLLACEAE margin on slope, 15 Jun 2018, E.B. Volynets 13283 (VLA). Melandrium album (Mill.) Garcke 2n = 24; CHN. Russia, Far East, Kamchatka Peninsula, Bystrins- Festuca kolymensis Drobow kii Raion, Esso town, 2018, O.A. Chernyagina 13322 (VLA). 2n = 42; CHN. Russia, Far East, Kamchatka Peninsula, Ust’- Kamchatskii Raion, SE macroslope of the Sopka Ploskaya Volcano, CONVOLVULACEAE small shrubby tundra on southern stony slope to the valley of Studë- *Convolvulus chinensis Ker Gawl. naya River, 1276 m, 23 Aug 2018, V.V. Yakubov 13324 (VLA); Kam- 2n = 48; CHN. Russia, East Siberia, Republic of Buryatia, Tar- chatka Peninsula, Ust’-Kamchatskii Raion, SE macroslope of the bagataiskii Raion, right riverside of the Selenga River, Voznesse- Sopka Ploskaya Volcano, meso-xerophytic meadows with Leonto- novka settlement, 503 m, roadside, weedy-ruderal plant community, podium on southern stony slope to the valley of Studënaya River, 13 Jul 2018, D.A. Krivenko 13356 (VLA). melkozem, 1162 m, 23 Aug 2018, V.V. Yakubov 13318 (VLA).

GERANIACEAE Hordeum jubatum L. Geranium dissectum L. 2n = 28; CHN. Russia, West Siberia, Altaiskii Krai, Pospeli- 2n = 22; CHN. Sweden, vicinity of Katrinholm town, near Mar- khinskii Raion, near Kotlyarovka settlement, weedy-ruderal plant morbyn village, Picea forest, on a huge stone, 02 Nov 2018, O.A. community, 20 Sep 2018, D.A. Krivenko 13409 (VLA). Chernyagina 13405 (VLA). Poa alpigena Lindm. Geranium pratense L. 2n = 56; CHN. Russia, Far East, Kamchatka Peninsula, Tigil’skii 2n = 28; CHN. Russia, East Siberia, Irkutskaya Oblast’, Slyu- Raion, southwards of Palana settlement, lower course of the Pyati- dyanskii Raion, W coast of Lake Baikal, 98th kilometer of the Kru- bratka River, roadside, 29 Jul 2018, O.A. Chernyagina 13393 (VLA). gobaikal’skaya railway road, right riverside of the Pylovka River, 485 m, margin of mixed forest, with miscellaneous herbs, 14 Sep Poa fischeri Prob. 2018, O.Yu. Zavgorodnyaya 13421 (VLA). 2n = 28; CHN. Russia, Far East, Kamchatka Peninsula, Tigil’s- kii Raion, Palana settlement, waste ground, 23 Jul 2018, O.A. Cher- LAMIACEAE nyagina 13333 (VLA). Prunella vulgaris L. 2n = 28; CHN. Sweden, vicinity of Katrinholm town, near Mar- Poa urssulensis Trin. morbyn village, margin of Picea forest, roadside, 02 Nov 2018, O.A. 2n = 28; CHN. Russia, Far East, Kamchatskii Krai, Penzhinskii Chernyagina 13401 (VLA). Raion, left riverside of the Penzhina River in its middle course, near

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Cape Krasnyi (northern extremity of Ushkanyi Ridge), 130 m, stony together with Raulinoa R.S.Cowan and Pilocarpus Vahl, into sub- slopes, abundant at rocks, 28 Jul 2018, V.V. Yakubov 13319 (VLA). tribe Pilocarpinae Engl. of the Galipeeae Kallunki tribe of the family Rutaceae (Pirani, 1999; Cruz & al., 2017). Esenbeckia has a wide dis- Schedonorus pratensis (Huds.) P.Beauv. tribution from Mexico to Argentina, and Metrodorea occurs in Bra- 2n = 14; CHN. Russia, Far East, Kamchatka Peninsula, Tigil’s- zil, Suriname and Bolivia (Kubitzki & al., 2011; Dias & al., 2015). kii Raion, Palana settlement, waste ground, 28 Jul 2018, O.A. Cher- However, both genera have higher specific diversity in the Brazilian nyagina 13400 (VLA). rainforests and seasonal forests. Particularly, the three species studied are endemic from three different phytogeographic domains: E. leio- PRIMULACEAE carpa from the Cerrado and Atlantic Forest domains, E. irwiniana Androsace septentrionalis L. from the Cerrado domain and M. concinna from the Caatinga domain 2n = 20; CHN. Russia, Far East, Kamchatka Peninsula, outskirts (Jardim Botânico do Rio de Janeiro, 2018). of Palana settlement, near the mouth of the Palana River, maritime Chromosome number studies are scarce for Esenbeckia and slope, forb meadow, 12 Jul 2018, O.A. Chernyagina 13321 (VLA). non-existent for Metrodorea. Thus, with the aim of increase the cytogenetic knowledge, chromosome number and heterochromatin char- Primula farinosa L. acterization were studied in E. irwiniana, E. leiocarpa and 2n = 18; CHN. Russia, Far East, Kamchatka Peninsula, outskirts M. concinna. of Palana settlement, near the mouth of the Palana River, maritime For mitotic chromosomes, root tips were pretreated with p- slope, forb meadow, 12 Jul 2018, O.A. Chernyagina 13399 (VLA). dichlorobenzene saturated solution for 2 h at room temperature, fixed in absolute ethanol/glacial acetic acid (3 : 1 v/v) for 12 h and RANUNCULACEAE stored at −20C. Root tips were digested in a 2% cellulase and Hepatica nobilis Mill. 20% pectinase solution at 37C for 30 min. For constitutive 2n = 14; CHN. Sweden, vicinity of Katrinholm town, near Mar- heterochromatin characterization, fluorescence banding (CMA/ morbyn village, margin of Picea forest, 02 Nov 2018, O.A. Chernya- DAPI) was used according to Schweizer & Ambros (1994) with gina 13344 (VLA). modifications. The somatic chromosome number found for E. irwiniana and VIOLACEAE E. leiocarpa was 2n = 64 (Fig. 5A,B) and for M. concinna was Viola tricolor L. 2n = ca. 54 (Fig. 5C). The metaphase chromosomes were relatively 2n = 26; CHN. Russia, Far East, Kamchatka Peninsula, Palana small and morphologically similar. The fluorescent banding showed settlement, owergrown dumping place, among buildings, 18 Jul two GC-rich regions (CMA+) at satellite level (Fig. 5), and the hetero- 2018, O.A. Chernyagina 13327 (VLA). chromatin pattern was conserved and almost identical for the three species with only differences in bands size. Esenbeckia irwiniana and E. leiocarpa presented the same chro- IAPT chromosome data 30/7 mosome number described for E. febrifuga (A.St.-Hil.) A.Juss ex Mart. (Kaastra, 1978), but differs from that observed by Guimarães María V.Romero-da Cruz,* José Rubens Pirani & Eliana Regina (2018) in E. irwiniana (2n = 56). However, in this last case, differ- Forni Martins ences in chromosome number must be an error in chromosome *Address for correspondence: [email protected] counting. For M. concinna, the chromosome number often observed was 2n = ca. 54. According to the reconstruction of chromosome Financial support from Brazilian agency: Fundação de Amparo number evolution in Rutaceae, Esenbeckia is in the clade with à Pesquisa do Estado de São Paulo (Grants 2016/065724 and x = 17 with a subsequent descending dysploidy to x =16 2014/180022). (Guimarães, 2018). Thus, the basic chromosome number for Esen- beckia could be postulated as x =8 or x =16(2n =8x =64or * First chromosome count for the species. 2n =4x = 64), but studies on a larger number of species are still nec- essary. In the case of Metrodorea, which is in the same clade with RUTACEAE Esenbeckia (Guimarães, 2018; Dias & al., in prep.), the basic chro- *Esenbeckia irwiniana Kaastra mosome number is more difficult to postulate. Unfortunately, there 2n=64, CNH. Brasil, Minas Gerais, Grão Mogol, 1633′21.9″S, are no chromosome data available for other genera closely related 04253′08.9″W, P. Dias 526 (ESA). to Metrodorea (i.e., Raulinoa, Helietta Tul. and Balfourodendron Corr.Mello ex Oliv.) (Dias & al., 2015; Cruz & al., 2017). Despite *Esenbeckia leiocarpa Engl. the chromosome number observed (2n = ca. 54) and that x = 9 is a 2n=64, CNH. Brasil, São Paulo, Campinas, 2253′59.9″S, frequent basic chromosome number in Rutaceae, this does not seem 4703′36″W, E.R.F. Martins & al. 30230 (UEC). to be the case for Metrodorea. A chromosome number higher than 2n = 54 appears as a more robust hypothesis. *Metrodorea concinna Pirani & P.Dias The heterochromatin is GC type with terminal position and asso- 2n=ca. 54, CNH. Brasil, Bahia, Jussiape, 1230′26.2″S, ciated to the NOR (nucleus organizing region). This pattern has 04111′06.8″W, P. Dias 557 (ESA). already been reported for several groups of plants (Guerra, 2000). The heterochromatin pattern of the Pilocarpinae species studied Esenbeckia Kunth and Metrodorea A.St.-Hil. are Neotropical seems to be conserved when compared to other Rutaceae species genera with 26 and 6 species, respectively. They have been classified, (Guerra, 1993; Moraes & al., 2007).

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Fig. 5. Mitotic chromosome metaphases of E. irwiniana, E. leiocarpa and M. concinna. Fluorescent chromosome banding with CMA/DAPI (CMA yellow fluorescence): A, Esenbeckia irwiniana; B, Esenbeckia leiocarpa; C, Metrodorea concinna. — Arrows indicate CMA+/DAPI– NOR heterochromatin. Scale bar = 10 μm.

LITERATURE CITED Schweizer, D. & Ambros, P.F. 1994. Chromosome banding: Stain Cruz, R., Duarte, M., Pirani, J.R. & Melo-de-Pinna, G.F.A. 2017. combinations for specific regions. Pp. 97–112 in: Gosden, Phylogenetic analysis and evolution of morphological characters J.R. (ed.), Chromosome analysis protocols. Methods in Molecu- in Metrodorea and related species in Rutoideae (Rutaceae). Pl. lar Biology 29. Totowa: Humana Press. https://doi.org/10.1385/ Syst. Evol. 303(7): 927–943. https://doi.org/10.1007/s00606- 0-89603-289-2:97 017-1423-x Dias, P., Udulutsch, R.G. & Pirani, J.P. 2015. Molecular phylogeny and biogeography of the South American genus Metrodorea IAPT chromosome data 30/8 (Rutaceae). Turkish J. Bot. 39: 825–834. https://doi.org/10. 3906/bot-1410-49 Manjit Inder Singh Saggoo & Mandeep Kaur Aulakh* Guerra, M. 1993. Cytogenetics of Rutaceae. V. High chromo- *Address for correspondence: [email protected] somal variability in Citrus species revealed by CMA/DAPI staining. Heredity 71: 234–241. https://doi.org/10.1038/hdy. The study was supported by financial grant under DSA I of 1993.131 UGC and UGC providing Senior Research Fellowship financial Guerra, M. 2000. Patterns of heterochromatin distribution in plant assistance to Mandeep Kaur Aulakh under BSR scheme for meritori- chromosomes. Genet. Molec. Biol. 23: 1029–1041. https://doi. ous students [award letter no. F. 5365]. org/10.1590/S1415-47572000000400049 Guimarães, G.S.R. 2018. Estudos citotaxonômicos em Sapin- * First report of B-chromosome for the species. dales: Estado da arte e evolução dos números cromossômicos. ** New chromosome report (cytotype) for the species. Dissertation, Universidade Estadual de Campinas, Campinas, Brasil. Cytological investigations have been carried out from the fronds Jardim Botânico do Rio de Janeiro 2018. Esenbeckia.In: with young sporangia fixed in Carnoy’s fixative (alcohol : chloro- Flora do Brasil 2020 em construção. Available at: http:// form : glacial acetic acid, 6 : 3 : 1, v/v) for 24 h at room temperature reflora.jbrj.gov.br/reflora/floradobrasil/FB572 (accessed 8 and then transferred to 70% alcohol and squashed in 1% Oct 2018). acetocarmine. Kaastra, R.C. 1978. [Reports]. In: Löve, Á. (ed.), IOPB chromo- Apogamy can be inferred if two distinct types of meiosis asso- some number reports LIX. Taxon 27: 53. https://doi.org/10. ciated with 8-celled and 16-celled sporangia, respectively, are 1002/j.1996-8175.1978.tb03513.x counted. In all the apogamous species, the chromosome count at Kubitzki, K., Kallunki, J.A., Duretto, M. & Wilson, P.W. 2011. meiosis is not half that of the rest of the plant body but is equal Rutaceae. Pp. 276–356 in: Kubitzki, K. (ed.), The families and to it, 2n = “n”. genera of vascular plants, vol. 10. Berlin: Springer. Moraes, A.P.,Santos, W.S.F. & Guerra, M. 2007. Karyotype diver- ADIANTACEAE sity and the origin of grapefruit. Chromosome Res. 15: 115–121. Adiantum capillus-veneris L. https://doi.org/10.1007/s10577-006-1101-2 n = 30, CHN. India, Haryana, Panchkula, Pinjore, Mughal Gar- Pirani, J.P. 1999. Estudos taxonômicos em Rutaceae: Revisão de dens, 3041′39.1524″N, 7651′38.034″E, 365 m, 25 May 2014, Helietta e Balfourodendron (Pteleinae), análise cladística de grown inside the wall crevices in shady places, M.K. Aulakh 32303 Pteleinae, sinopse de Rutaceae no Brasil. Dissertation, Univer- (PUN 4827) [Fig. 6A]. sidade de São Paulo, São Paulo, Brasil.

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Fig. 6. A, Adiantum capillus-veneris, SMC at metaphase I, n = 30 (PUN 4827); B, Adiantum capillus-veneris, SMC at metaphase I, n = 30+1B (arrow) (PUN 4834); C, Adiantum caudatum, SMC at diakinesis, n = 30 (PUN 4873); D, Adiantum lunulatum, SMC at diakinesis, 2n = “n” = 90 (PUN 4848); E, Asplenium trichomanes, SMC at metaphase I, n = 36 (PUN 4875); F, Asplenium trichomanes, SMC at metaphase I, n = 72 (PUN 4876); G, Asplenium trichomanes, SMC at metaphase I, n = 72+1B (arrow) (PUN 4876); H, Polystichum squarrosum, SMC at metaphase I, n = 41 (PUN 4877); I, Polystichum squarrosum, SMC at diakinesis, 2n =3x = 123 (PUN 4849); J, Polystichum squarrosum, SMC at diakinesis, 2n =3x = 123+1B (arrow) (PUN 4850); K, Pteris cretica, SMC at metaphase I, 2n = “n” = 58 (PUN 4839); L, Pteris cretica, SMC at metaphase I, 2n = “n” = 58+1B (arrow) (PUN 4840); M, Pteris cretica, SMC at metaphase I, 2n = “n” = 87 (PUN 4842); N, Pteris cretica, SMC at diakinesis, 2n = “n” = 87+1B (arrow) (PUN 4842); O, Glaphyropteridopsis erubescens, SMC at metaphase I, n = 36 (PUN 4874). — Scale bars = 10 μm.

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*n = 30+1B, CHN. India, Himachal Pradesh, Kullu, Naggar, 2n = “n” = 87, CHN. India, Himachal Pradesh, Chamba, Dal- Raison, 3230′17.4744″N, 7727′57.7092″E, 1435 m, 09 Jul 2014, housie, Bathri, 3234′22.728″N, 7558′46.938″E, 1720 m, 22 Sep grown in moist and shady area along streams, M.K. Aulakh 32310 2015, grown in disturbed shady area on the way to forests, M.K. (PUN 4834) [Fig. 6B]. Aulakh 32319 (PUN 4842) [Fig. 6M]. 2n = “n” = 87+1B, CHN. India, Himachal Pradesh, Chamba, Adiantum caudatum L. Dalhousie, Bathri, 3234′22.728″N, 7558′46.938″E, 1720 m, n = 30, CHN. India, Himachal Pradesh, Kangra, Samela, 3246′ 22 Sep 2015, grown in disturbed shady area on the way to forests, 55.9128″N, 7615′21.1608″E, 666 m, 04 Sep 2014, grown on open M.K. Aulakh 32319 (PUN 4842) [Fig. 6N]. slopes, M.K. Aulakh 32368 (PUN 4873) [Fig. 6C]. THELYPTERIDACEAE Adiantum lunulatum Burm.f. Glaphyropteridopsis erubescens (Wall.ex Hook.) Ching 2n = “n” = 90, CHN. India, Himachal Pradesh, Dharamshala, n = 36, CHN. India, Himachal Pradesh, Kullu, Parvati Valley, Man- Mcleodganj, Bhagsunag, 3214′23.1324″N, 7619′41.3472″E, ikaran, 3215′36.462″N, 7721′39.9816″E, 1760 m, 14 Sep 2017, grown 1896 m, 26 Jul 2016, grown in extremely moist and shady rock crev- on moist and open slopes, M.K. Aulakh 32369 (PUN 4874) [Fig. 6O]. ices, M.K. Aulakh 32360 (PUN 4848) [Fig. 6D]. LITERATURE CITED ASPLENIACEAE Ghatak, J. 1977. Biosystematic survey of Pteridophyta from Asplenium trichomanes L. Shevaroy Hills, South India. Nucleus (Calcutta) 20: 105–108. n = 36, CHN. India, Himachal Pradesh, Chamba, Dalhousie, Khullar, S.P. & Gupta, S.C. 1978. Cytotaxonomy of the genus Subhash Baoli, 3232′19.4604″N, 7558′15.5928″E, 2021 m, Polystichum in the western Himalayas. Pl. Syst. Evol. 129: 269–275. 18 Sep 2015, grown in moist and dense forests, M.K. Aulakh 32370 Khullar, S.P., Sharma, S.S. & Verma, S.C. 1988. [Report]. In: Bir, (PUN 4875) [Fig. 6E]. S.S. (ed.), SOCGIplant chromosome number reports VI. J.Cytol. n = 72, CHN. India, Himachal Pradesh, Chamba, Nainikhad, Genet. 23: 38–50. https://doi.org/10.1007/BF00982753 3229′30.7968″N, 7555′28.3764″E, 1350 m, 05 Aug 2013, grown Mehra, P.N. & Khullar, S.P. 1980. Cytotaxonomy of western Hima- on moist open slopes along streams, M.K. Aulakh 32371 (PUN layan ferns II, Gleicheniaceous series. Res. Bull. Punjab Univ., 4876) [Fig. 6F]. New Ser., Sci. 25: 135–178. *n = 72+1B, CHN. India, Himachal Pradesh, Chamba, Naini- Punetha, N. & Sen, A. 1989. Cytological observations on some spe- khad, 3229′30.7968″N, 7555′28.3764″E, 1350 m, 05 Aug 2013, cies of Pteris from Kumaon (West Himalaya). Proc. Indian grown on moist open slopes along streams, M.K. Aulakh 32371 Acad. Sci., Pl. Sci. 99: 131–134. (PUN 4876) [Fig. 6G]. Verma, S.C. 1961. Chromosome numbers in Himalayan Ferns. Res. Bull. Punjab Univ., New Ser., Sci. 12: 139–164. DRYOPTERIDACEAE Verma, S.C. & Loyal, D.S. 1960. Chromosome counts in some ferns Polystichum squarrosum (D.Don) Fée from Nainital. Curr. Sci. 29: 69–70. n = 41, CHN. India, Himachal Pradesh, Shimla, Matiana, 3112′ 36.5346″N, 7724′20.322″E, 2650 m, 25 Sep 2016, grown as wayside vegetation along the streams, M.K. Aulakh 32372 (PUN 4877) IAPT chromosome data 30/9 [Fig. 6H]. **2n =3x = 123, CHN. India, Himachal Pradesh, Shimla, Mati- Fabiano J. Santos, Géssica S. Santos, Pamela C.S.S. Souza, ana, 3112′36.5346″N, 7724′20.322″E, 2650 m, 25 Sep 2016, Samara S. Matos, André L. Melo, Vera L.C. Vale & grown as wayside vegetation along the streams, M.K. Aulakh Maria J. Gomes de Andrade* 32365 (PUN 4849) [Fig. 6I]. *Address for correspondence: [email protected] *2n =3x = 123+1B, CHN. India, Himachal Pradesh, Shimla, Matiana, 3112′36.5346″N, 7724′20.322″E, 2650 m, 25 Sep 2016, Financial support from Coordenação de Aperfeiçoamento de grown as wayside vegetation along the streams, M.K. Aulakh Pessoal de Nível Superior (CAPES), Conselho Nacional de Desen- 32366 (PUN 4850) [Fig. 6J]. volvimento Científico e Tecnológico (CNPq), and Fundação de Previously, the species was known to have chromosome counts Apoio à Pesquisa do Estado da Bahia (FAPESB, process of n = 41 (Verma & Loyal, 1960; Verma, 1961; Khullar & Gupta, no. PIE0023/2016 for M.J.G. Andrade). 1978; Mehra & Khullar, 1980; Khullar & al., 1988; Punetha & Sen, 1989) and n = 82 (Ghatak, 1977). * First chromosome count for the species.

PTERIDACEAE Chromosome counts were performed following the method by Pteris cretica L. Guerra & Souza (2002). 2n = “n” = 58, CHN. India, Himachal Pradesh, Shimla, Summer Hill, 3116′52.4952″N, 7710′23.5968″E, 2100 m, 10 Sep 2013, in LEGUMINOSAE shade in forest wet undergrowths, M.K. Aulakh 32316 (PUN 4839) Subfamily Papilionoideae [Fig. 6K]. Tribe Dalbergieae (DC.) Cardoso & al. *2n = “n” = 58+1B, CHN. India, Himachal Pradesh, Kangra, Zornia leptophylla (Benth.) Pittier Ranital, 3209′58.1508″N, 7613′31.6596″E, 680 m, 03 Sep 2014, *2n = 20, CHN. Brazil, Bahia, Paulo Afonso, road towards the on moist rocky slopes near water courses, M.K. Aulakh 32317 Belvedere Park, 0923′31″S, 3812′76″W, 29 Jun 2018, F.J. Santos (PUN 4840) [Fig. 6L]. 56 (HUNEB) [Figs. 7A, 8A].

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Fig. 7. Flowers of some of the analyzed species. A, Zornia leptophylla; B, Indigofera hirsuta; C, I. microcarpa; D, I. suffruticosa; E, Lonchocarpus sericeus; F, Tephrosia purpurea; G, Ancistrotropis peduncularis; H, Centrosema pascuorum; I, Clitoria fairchildiana; J, Macroptilium atropurpureum; K, M. lathyroides; L, Rhynchosia minima. — Photos: A–E, G, H, J–L by F.J. Santos; F by A.L. Melo; I by G.S. Santos.

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Fig. 8. Chromosome complements in some species of the Papilionoideae. A, Zornia leptophylla,2n = 20; B, Indigofera hirsuta,2n = 16; C, I. microcarpa,2n = 16; D, I. suffruticosa,2n = 16; E, Lonchocarpus sericeus,2n = 22; F, Tephrosia purpurea,2n = 22; G, Ancis- trotropis peduncularis,2n = 26; H, Centrosema pascuorum,2n=22; I, Clitoria fairchildiana,2n=22; J, Macroptilium atropurpureum,2n=22; K, M. lathyroides,2n=22; L, Rhynchosia minima,2n=22. — Arrows in F, I and L indicate satellites. Scale bar = 10 μm.

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Tribe Indigofereae (Benth.) Hutch. 2017). Most species caryologically studied in the subfamily present Indigofera hirsuta L. 2n = 16, 18, 20 and 22 as chromosome number. However, 2n =12, 2n = 16, CHN. Brazil, Pernambuco, Serra Talhada, Mata da 14, 24, 26, 28, 30, 32, 38, 40, 48, 64 and 84 are also reported Pimenteira State Park, 0757′31″S, 3817′77″W, 22 Nov 2017, F.J. (LPWG, 2017). Consequently, different basic chromosome numbers Santos 19 (HUNEB) [Figs. 7B, 8B]. are reported for Papilionoideae: x = 8, 10, 11, 12, 13, 14, 18 and 20 (Goldblatt, 1981; Poggio & al., 2008). In this work, 12 species repre- Indigofera microcarpa Desv. senting 4 tribes were studied: Dalbergieae (DC.) Cardoso & al., Indigo- 2n = 16, CHN. Brazil, Bahia, Paulo Afonso, Belvedere Park, fereae (Benth.) Hutch., Millettieae Miq. and Phaseoleae (Bronn) DC. 0923′55″S, 3812′47″W, 29 Jun 2018, F.J. Santos 54 (HUNEB) Dalbergieae comprises 1331 species in 49 genera, of which [Figs. 7C, 8C]. 25 have chromosome counts, covering 298 species. For only 12 out of the 74 Zornia J.F.Gmel. species, chromosomes are counted Indigofera suffruticosa Mill. (Lewis & al., 2005; Rice & al., 2015), all having 2n = 20, except 2n = 16, CHN. Brazil, Bahia, Paulo Afonso, road towards the for Z. diphylla (L.) Pers., for which Sareen & Trehan (1977) observed Belvedere Park, 0923′31″S, 3812′76″W, 07 May 2016, G.S. Santos 2n = 22, different from 12 other records of 2n = 20 for this species, 115 (HUNEB) [Figs. 7D, 8D]. and Z. sericea Moric., for which Gill & Husaini (1986) reported n = 11, different from the single other record of 2n = 20 (Seijo Tribe Millettieae Miq. & Vanni, 1999). In this study, the chromosome number 2n =20 Lonchocarpus sericeus (Poir.) Kunth ex DC. observed for Z. leptophylla corresponds to the first karyotypic record 2n = 22, CHN. Brazil, Pernambuco, Serra Talhada, Mata da for this species, confirming the numerical stability for the genus and Pimenteira Park, 0757′31″S, 3817′77″W, 23 Apr 2018, F.J. Santos the basic number x = 10 mentioned in the literature for this tribe. 13 (HUNEB) [Figs. 7E, 8E]. Indigofereae has approximately 768 species in 7 genera. Seven hundred species belong to Indigofera L. For this genus, chromosome Tephrosia purpurea (L.) Pers. counts for 147 species exist (Rice & al., 2015; Tozzi, 2016). The 2n = 22, CHN. Brazil, Bahia, Paulo Afonso, Belvedere Park, numbers 2n = 14, 16, 32, 48 are recorded in the literature for this 0923′55″S, 3812′47″W, 20 Jun 2018, F.J. Santos 48 (HUNEB) genus (Goldblatt, 1981; Polhill, 1981), 2n = 16 being the most fre- [Figs. 7F, 8F]. quent. In this study, 2n = 16 was reported for the three species of Indi- gofera analyzed, corroborating the previous records for these same Tribe Phaseoleae (Bronn) DC. taxa in the works of Goldblatt (1981) and Polhill (1981), as well as Ancistrotropis peduncularis (Fawc. & Rendle) A.Delgado the basic number x = 8 of the tribe (Kumari & Bir, 1990). *2n = 26, CHN. Brazil, Bahia, Euclides da Cunha, Curral Falso, Millettieae is composed of 45 genera and 909 species, and the 1025′75″S, 3905′28″W, 12 Sep 2015, G.S. Santos 172 (HUNEB) most commonly observed chromosome numbers are 2n =20or [Figs. 7G, 8G]. 22 (Schrire, 2005; Doyle, 2012). Tephrosia has 350 species, of which 54 have chromosome counts (Schrire, 2005; Rice & al., Centrosema pascuorum Mart. ex Benth. 2015), while Lonchocarpus has 120 species, being the richest genus 2n = 22, CHN. Brazil, Bahia, Paulo Afonso, Military Area, of the Millettieae tribe in the Neotropical region (Silva & Tozzi, 0923′45″S, 3813′96″W, 12 Jul 2016, G.S. Santos 134 (HUNEB) 2012), with records of chromosome counts for 57 species. Both [Figs. 7H, 8H]. Table 1. Chromosome measurements of the species studied. Clitoria fairchildiana R.A.Howard 2n = 22, CHN. Brazil, Bahia, Paulo Afonso, Universidade do Chromosome μ Estado da Bahia, Caminho das Águas, 0940′07″S, 3821′29″W, size ( m) 07 Jul 2017, F.J. Santos 01 (HUNEB) [Figs. 7I, 8I]. Species CTLH* (μm) Smaller Bigger Ancistrotropis peduncularis 16.04 0.71 2.01 Macroptilium atropurpureum (DC.) Urb. 2n = 22, CHN. Brazil, Bahia, Paulo Afonso, Capuxu Lake, Centrosema pascuorum 13.40 1.07 1.76 ′ ″ ′ ″ 09 23 76 S, 38 12 54 W, 29 Jun 2018, F.J. Santos 51 (HUNEB) Clitoria fairchildiana 35.96 1.32 4.41 [Figs. 7J, 8J]. Indigofera hirsuta 18.16 1.20 3.13 Macroptilium lathyroides (L.) Urb. Indigofera microcarpa 14.16 1.08 2.34 2n = 22, CHN. Brazil, Bahia, Paulo Afonso, Military Area, 0923′93″S, 3812′09″W, 11 Apr 2016, G.S. Santos 110 (HUNEB) Indigofera suffruticosa 17.34 2.09 3.07 [Figs. 7K, 8K]. Lonchocarpus sericeus 49.31 3.32 6.36

Rhynchosia minima (L.) DC. Macroptilium atropurpureum 28.26 1.67 4.51 2n = 22, CHN. Brazil, Pernambuco, Serra Talhada, Mata da Macroptilium lathyroides 14.23 0.89 1.94 Pimenteira Park, 0757′31″S, 3817′77″W, 23 Apr 2018, F.J. Santos Rhynchosia minima 34.70 1.95 5.08 45 (HUNEB) [Figs. 7L, 8L]. Tephrosia purpurea 61.80 3.59 9.71 Papilionoideae is the largest and most derived subfamily of Legu- Zornia leptophylla 22.83 1.54 3.37 minosae, comprising 14,000 species in 503 genera, accounting for 59% of the genera and 69% of Leguminosae species (Doyle, 2012; LPWG, * CTLH = Total Haploid Set Length.

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species of the tribe analyzed in this study have 2n = 22, confirming Polhill, R.M. 1981. Indigofereae. Pp. 289–291 in: Polhill, previous counts for Lonchocarpus sericeus and Tephrosia purpurea R.M. & Raven, P.H. (eds.), Advances in legume systematics, (Kumari & Bir, 1990; Palomino & Sousa, 2000; Rice & al., 2015) part 1. Richmond: Royal Botanic Gardens, Kew. and corroborating the basic number x = 8 for these genera. The pres- Rice, A., Glick, L., Abadi, S., Einhorn, M., Kopelman, N.M., ence of a satellite in the cells of T. purpurea (Fig. 8F) was observed. Salman-Minkov, A., Mayzel, J., Chay, O. & Mayrose, I. Phaseoleae has 89 genera and about 1580 species, some of 2015. The Chromosome Counts Database (CCDB) – A commu- which are of economic interest (Lewis & al., 2005). In this tribe, nity resource of plant chromosome numbers. New Phytol. 206: 2n = 20 or 22 are the chromosome numbers found in caryological 19–26. https://doi.org/10.1111/nph.13191 records of most species (Doyle, 2012). In this sense, the number Santos, G.S. 2017. Citogenética de espécies de Leguminosae Juss. 2n = 22 observed in this work for Centrosema pascuorum, Clitoria ocorrentes na Caatinga da porção sul da Ecorregião Raso da fairchildiana, Macroptilium atropurpureum, M. lathyroides and Catarina, Bahia, Brasil. Dissertation, Universidade do Estado Rhynchosia minima does not differ from the literature (Sinha & da Bahia, Paulo Afonso, Brasil. Roy, 1979; Pedrosa & al., 1999; Santos, 2017). However, for Ancis- Sareen, T.S. & Trehan, R. 1977. Cytological studies in some Papi- trotropis peduncularis, the number 2n = 26 was observed. It is the lionaceae. Proc. Indian Sci. Congr. Assoc. 64: 156. first chromosome record for this species. In the cell of C. fairchildi- Schrire, B.D. 2005. Tribe Millettieae. Pp. 367–445 in: Lewis, G.P., ana, two chromosomes were observed with satellite (Fig. 8I); in Schrire, B.D, Mackinder, B.A. & Lock, M. (eds.), Legumes of Rhynchosia minima, there were three satellites (Fig. 8L). the world. Richmond: Royal Botanic Gardens, Kew. All species analyzed had semi-reticulated interphase nuclei and Seijo, G. & Vanni, R. 1999. Números cromosómicos en leguminosas a proximal prophase condensation pattern, characteristics common to de Paraguay. Bol. Soc. Argent. Bot. 34: 119–122. Leguminosae. Chromosome measurements were performed, and the Silva, M.J. & Tozzi, A.M.G.A. 2012. Revisão taxonômica de Total Haploid Set Length (CTLH, Table 1) was calculated. Tephrosia Lonchocarpus s.str. (Leguminosae, Papilionoideae) do Brasil. purpurea had the highest CTLH (61.8 μm), and Centrosema pas- Acta Bot. Brasil. 26: 357–377. https://doi.org/10.1590/S0102- cuorum had the lowest CTLH (13.4 μm). With respect to variation 33062012000200012 in chromosome length, T. purpurea had the highest amplitude Sinha, S.S.N. & Roy, H. 1979. Cytological studies in the (6.12 μm), and C. pascuorum had the lowest amplitude (0.69 μm). genus Phaseolus. I. Mitotic analysis in fourteen species. These data are important because they indicate the estimated amount Cytologia 44: 191–199. https://doi.org/10.1508/cytologia. of DNA, since the species with the highest CTLH may have a higher 44.191 DNA content (Heslop-Harrison & Schwarzacher, 2011). Tozzi, A.M.G.A. 2016. Subfamília Papilionoideae. Pp. 167–397 in: Melhem, T.S., Forero, E., Fortuna-Perez, A.P., Wanderley, LITERATURE CITED M.G.L., Martins, S.E., Romanini, R.P., Pirani, J.R., Fiuza de Doyle, J.J. 2012. Polyploidy in legumes. Pp. 147–180 in: Soltis, Melo, M.M.R., Kirizawa, M., Yano, O. & Cordeiro, I. (eds.), P.S. & Soltis, D.E. (eds.), Polyploidy and genome evolution.Berlin, Flora fanerogâmica do Estado de São Paulo, vol. 8. São Paulo: Heidelberg: Springer. https://doi.org/10.1007/978-3-642-31442-1_9 Instituto de Botânica. Gill, L.S. & Husaini, S.W. 1986. Cytological observations in Legu- minosae from southern Nigeria. Willdenowia 15: 521–527. Goldblatt, P. 1981. Chromosome numbers in legumes II. Ann. IAPT chromosome data 30/10 Missouri Bot. Gard. 68: 551–557. https://doi.org/10.2307/2398889 Guerra, M. & Souza, M.J. 2002. Como observar cromossomos: Pollyana K. Silva, Lânia I.F. Alves,* José Achilles L. Neves, Um guia de técnicas em citogenética vegetal, animal e Erton Mendonça de Almeida, Enoque Medeiros Neto, humana. Ribeirão Preto, SP: FUNPEC. Fabiane R.C. Batista, Daniela C. Zappi & Leonardo P. Felix Heslop-Harrison, J.S. & Schwarzacher, T. 2011. Organisation of *Address for correspodence: [email protected] the plant genome in chromosomes. Plant J. 66: 18–33. https:// doi.org/10.1111/j.1365-313X.2011.04544.x Financial support from CNPq (Conselho Nacional de Desenvol- Kumari, S. & Bir, S.S. 1990. Karyomorphological evolution in vimento Científico e Tecnológico), UFPB (Universidade Federal da Papilionaceae. J. Cytol. Genet. 25: 173–219. Paraíba), INSA (Instituto Nacional do Semiárido). Lewis, G.P., Schrire, B.D., Mackinder, B.A. & Lock, M. (eds.) 2005. Legumes of the World. Kew: Royal Botanic Gardens. Methods for chromosome analysis are according to Guerra LPWG [Legume Phylogeny Working Group] 2017. A new sub- & Souza (2002). family classification of the Leguminosae based on a taxonomi- cally comprehensive phylogeny. Taxon 66: 44–77. https://doi. * First chromosome count for the genus. org/10.12705/661.3 ** First chromosome count for the species. Palomino, G. & Sousa, S.M. 2000. Variation of nuclear DNA content *** First chromosome count for the subspecies. in the biflorus species of Lonchocarpus (Leguminosae). Ann. Bot. (Oxford) 85: 69–76. https://doi.org/10.1006/anbo.1999.0998 CACTACEAE Pedrosa, A., Gitai, J., Silva, A.E.B., Felix, L.P. & Guerra, M. Subfamily Cactoideae 1999. Citogenética de angiospermas coletadas em Pernambuco Tribe Cereeae – V. Acta Bot. Brasil. 13: 49–60. https://doi.org/10.1590/ **Coleocephalocereus aureus F.Ritter S0102-33061999000100006 2n = 22, CNH. Brazil, Minas Gerais, Pedra Azul, 1603′08″S, Poggio, L., Espert, S.M. & Fortunato, R.H. 2008. Citogenética 4114′37″W, 708 m, 17 Dec 2016, E.M. Almeida & al. 1970 evolutiva en leguminosas americanas. Rodriguésia 59: (EAN) [Figs. 9A, 10A]. 423–433. https://doi.org/10.1590/2175-7860200859301

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***Coleocephalocereus decumbens F.Ritter ***Pilosocereus brasiliensis subsp. ruschianus (Buining & 2n = 22, CNH. Brazil, Minas Gerais, Padre Paraíso, 1708′21″S, Brederoo) Zappi 4129′49″W, 630 m, 19 Dec 2016, E.M. Almeida & al. 2025 (EAN) 2n = 22, CNH. Brazil, Bahia, Vitória da Conquista, 1504′06″S, [Figs. 9B, 10B]. 4044′07″W, 474 m, 16 Dec 2016, E.M. Almeida & al. 1965 (EAN) [Figs. 11A, 13A]. **Coleocephalocereus goebelianus (Vaupel) Buining 2n = 22, CNH. Brazil, Bahia, Jussiape, 1327′17″S, 4134′27″W, ***Pilosocereus catingicola (Gürke) Byles & G.D.Rowley 614 m, 22 Dec 2016, E.M. Almeida & al. 2091 (EAN) [Figs. 9C, subsp. catingicola 10C]. 2n = 44, CNH. Brazil, Bahia, Miguel Calmon, 1123′04″S, 4036′03″W, 541 m, 06 Dec 2016, E.M. Almeida & al. 1760 **Coleocephalocereus purpureus (Buining & Brederoo) F.Ritter (EAN) [Figs. 11B, 13B]. 2n = 22, CNH. Brazil, Minas Gerais, Itinga, 1635′49″S, 4143′ 43″W, 287 m, 18 Dec 2016, E.M. Almeida & al. 2013 (EAN) [Figs. 9D, 10D].

Fig. 9. Coleocephalocereus metaphases CMA/DAPI studied, 2n = 22. A, C. aureus; B, C. decumbens; dashed lines indicate the distention of terminal CMA+ bands; C, C. goebelianus; D, C. purpureus.

Fig. 10. Selection of analyzed Coleocepha- locereus taxa. A, C. aureus; B, C. decumbens; C, C. goebelianus; D, C. purpureus. — Photos by E.M. Almeida, J.A.L. Neves and F. Lavorato.

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***Pilosocereus catingicola subsp. salvadorensis (Werderm.) Zappi ***Pilosocereus gounellei subsp. zehntneri (Britton & Rose) 2n = 44, CHN. Brazil, Paraíba, Rio Tinto, 0647′28″S, 3455′10″W, Zappi 5 m, 18 Mar 2016, E.M. Almeida & al. 1616 (EAN) [Figs. 11C, 13C]. 2n = 22, CHN. Brazil, Bahia, Ourolândia, 1100′28″S, 4120′ 36″W, 768 m, 07 Dec 2016, E.M. Almeida & al. 1777 (EAN) Pilosocereus chrysostele (Vaupel) Byles & G.D.Rowley [Figs. 12C, 13H]. 2n = 22, CHN. Brazil, Paraíba, Assunção, 0711′58″S, 3649′24″W, 556 m, 16 Mar 2016, E.M. Almeida & al. 1613 (EAN) [Figs. 11D, 13D]. **Pilosocereus magnificus (Buining & Brederoo) F.Ritter 2n = 22, CHN. Brazil, Minas Gerais, Itinga, 1635′49″S, 4143′ ***Pilosocereus floccosus subsp. quadricostatus (F.Ritter) Zappi 43″W, 287 m, 18 Dec 2016, E.M. Almeida & al. 2011 (EAN) 2n = 22, CHN. Brazil, Minas Gerais, Pedra Azul, 1603′08″S, [Figs. 12D, 13I]. 4114′37″W, 708 m, 17 Dec 2016, E.M. Almeida & al. 1971 (EAN) [Figs. 11E, 13E]. **Pilosocereus multicostatus F.Ritter 2n = 22, CHN. Brazil, Minas Gerais, Pedra Azul, 1603′08″S, **Pilosocereus glaucochrous (Werderm.) Byles & G.D.Rowley 4114′37″W, 708 m, 17 Dec 2016, E.M. Almeida & al. 1976 (EAN) 2n = 22, CHN. Brazil, Bahia, Morro do Chapéu, 1134′12″S, [Figs. 12E, 13J]. 4107′31″W, 942 m, 06 Dec 2016, E.M. Almeida & al. 1755 (EAN) [Figs. 11F, 13F]. Pilosocereus pachycladus F.Ritter subsp. pachycladus 2n = 44, CHN. Brazil, Minas Gerais, Pedra Azul, 1559′02″S, ***Pilosocereus gounellei (F.A.C.Weber ex K.Schum.) Byles 4123′05″W, 699 m, 18 Dec 2016, E.M. Almeida & al. 2000 & G.D.Rowley subsp. gounellei (EAN) [Figs. 12F, 13K]. 2n = 22, CHN. Brazil, Paraíba, Algodão de Jandaíra, 0653′10″S, 3557′20″W, 476 m, 10 Mar 2016, E.M. Almeida & al. 1591 (EAN) [Figs. 12A,B, 13G].

Fig. 11. Pilosocereus metaphases CMA/ DAPI studied, 2n = 22 and 44. A, P. brasiliensis subsp. ruschianus; B, P. catingicola subsp. catingicola; C, P. catingicola subsp. salvadorensis; D, P. chrysostele; E, P. floccosus subsp. quadricostatus; F, P. glaucochrous.

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***Pilosocereus pentaedrophorus (Labour.) Byles & Cereeae Salm.Dyck, such as Arrojadoa Britton & Rose, Cereus G.D.Rowley subsp. pentaedrophorus Mill., Coleocephalocereus Backeb., Discocactus Pfeiff., Pilosocer- 2n = 22, CHN. Brazil, Pernambuco, Brejo da Madre de Deus, eus Byles & G.D.Rowley and Melocactus Link & Otto, with the last 0810′02″S, 3622′08″W, 770 m, 05 May 2016, E.M. Almeida two being the most speciose and of rather difficult specific delimita- & al. 1654 (EAN) [Figs. 12G, 13L]. tion. The genera studied here, Coleocephalocereus and Pilosocereus, are nowadays part of a group of a clade known as BCT (Hernandez- **Pilosocereus tuberculatus (Werderm.) Byles & G.D.Rowley Hernandez & al., 2011), including former members of Browningiae, 2n = 22, CHN. Brazil, Pernambuco, Buíque, 0831′53″S, 3715′ Cereeae and Trichocereeae tribes, and predominantly of South Ameri- 01″W, 926 m, 07 May 2016, E.M. Almeida & al. 1681 (EAN) can origin. Chromosome characters may be incorporated as informa- [Figs. 12H, 13M]. tive data to aid in the identification of species and as additional characters in phylogenetic analysis, and their configurations may also Eastern Brazil is a major centre of diversity of the family Cacta- be reflected in the possibilities of cultivated plants improvement ceae (Taylor & Zappi, 2004, 2008) with high levels of endemism (Stace, 2000). The chromosome number is a widely employed param- namely in genera formerly considered as members of the tribe eter in plant cytology and, used in association with other caryological

Fig. 12. Pilosocereus metaphases CMA/ DAPI studied, 2n = 22 and 44. A, Interphase nuclei, P. gounellei subsp. gounellei; B, P. gounellei subsp. gounellei; C, P. gounellei subsp. zehntneri; D, P. magnificus; E, P. multicostatus; F, P. pachycladus subsp. pachycladus; G, P. pentaedrophorus subsp. pentaedrophorus; H, P. tuberculatus.

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characters, such as heterochromatic patterns, may help in species CMA/DAPI bands in species of Coleocephalocereus and Pilosocer- delimitation and in the understanding of their evolution (Stebbins, eus, in order to identify heterochromatine band patterns that may be 1971; Guerra, 2000). Cytogenetic characterization using double color- characteristic for these plant groups. ation (CMA/DAPI) has allowed for detailed chromosome analyses of Thus, root tips were pretreated with 8-hydroxyquinoline plant caryotypes (Guerra, 2005), providing considerable information 0.002 M at 4C for 24 h, fixed in Carnoy 3 : 1 absolute ethanol/gla- for taxonomy (Brammer & al., 2015). This study aimed at document- cial acetic acid (v/v) for 3 h at room temperature and subsequently ing chromosome number variation and to characterize patterns of stored in freezer at −20C. To prepare the slides, the root tips were

Fig. 13. Selection of analyzed Pilosocereus taxa. A, P. brasiliensis subsp. ruschianus; B, P. catingicola subsp. catingicola; C, P. catingicola subsp. salvadorensis; D, P. chrysostele; E, P. floccosus subsp. quadricostatus; F, P. glaucochrous; G, P. gounellei subsp. gounellei; H, P. gounellei subsp. zehntneri; I, P. magnificus; J, P. multicostatus; K, P. pachycladus subsp. pachycladus; L, P. pentaedrophorus subsp. pentaedrophorus; M, P. tuberculatus. — Photos: E.M. Almeida, J.A.L. Neves and F. Lavorato.

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Table 2. Chromosomal data of the tribe Cereeae (Cactoideae–Cactaceae) species studied. Taxa 2n Variation of chromosome size (μm) Banding* CMA+/DAPI– Previous counts** Coleocephalocereus

C. aureus 22 0.81–1.52 2t+2pe – C. decumbens 22 0.82–1.71 2t+2pe – C. goebelianus 22 0.79–1.74 2t+2pe – C. purpureus 22 0.81–1.60 2t+2pe – Pilosocereus Byles & Rowley P. brasiliensis subsp. ruschianus 22 1.72–2.80 2t – P. catingicola subsp. catingicola 44 1.82–2.55 4t – P. catingicola subsp. salvadorensis 44 1.47- 2.60 4t+44pe – P. chrysostele 22 1.24–2.30 2t A14; C13; C16 P. floccosus subsp. quadricostatus 22 2.10–3.20 2t+22pe – P. glaucochrous 22 1.11–1.63 2t – P. gounellei subsp. gounellei 22 2.12–2.70 2t – P. gounellei subsp. zehntneri 22 1.30–2.20 2t – P. magnificus 22 1.54–2.60 2t – P. multicostatus 22 1.43–1.76 2t – P. pachycladus subsp. pachycladus 44 0.90–1.62 4t A14; C13; C16 P. pentaedrophorus subsp. pentaedrophorus 22 1.16–1.45 2t – P. tuberculatus 22 1.50–1.94 2t+22pe – * Chromosome banding: i = interstitial; pe = pericentromeric; t = terminal ** Previous counts: A14 = Assis & al., 2014; C13 = Castro & al., 2013; C16 = Castro & al., 2016

washed twice in distilled water and digested in an enzymatic solution from remaining species studied by their heterochromatic pericentro- containing 2% cellulase and 20% pectinase, and kept in a moist cham- meric CMA+ bands (Figs. 11E, 12H, 11C, respectively). ber at 37C for 1 h. The slides were aged for three days at room temperature and then stained for 1 h with 10 μl CMA (0.1 mg/ml) and LITERATURE CITED then with 10 μl DAPI (2 mg/ml) for 30 min, mounted in glycerol/ Assis, J.G.A., Senra, J.F.V., Botelho, R.B., Santos, McIlvaine buffer (pH 7.0) (1 : 1, v/v) and then stored for three days A.B. & Schnadelbach, A.S. 2014. Variação cromossômica in the dark for fluorochromes stabilization (Guerra & Souza, 2002). em Cactáceas. Ci. & Nat. 36: 267–278. https://doi.org/10. The best metaphases were captured in photomicroscope Zeiss with 5902/2179460X13189 Axio Cam MRC5 using Axiovision v.4.8 software. To measure the Brammer, S.P.,Toniazzo, C. & Poersch, L.B. 2015. Corantes comu- chromosomes, imageJ v.1.52p software was used. mente empregados na citogenética vegetal. Arq. Inst. Biol. (São Coleocephalocereus and Pilosocereus, both belonging to tribe Cer- Paulo) 82: 1–8. https://doi.org/10.1590/1808-1657000192013 eeae were analysed. Coleocephalocereus is endemic to eastern Brazil Castro, J.P., Souza, L.G.R., Alves, L.I.F., Silva, A.E.B., Guerra, and includes nine species, of which we analysed four (Coleocephalocer- M. & Felix, L.P. 2013. [Report]. In: Marhold, K. (ed.), IAPT/ eus aureus, C. decumbens, C. goebelianus, C. purpureus). All species IOPB chromosome data 15. Taxon 62: 1073, E1–E6. https:// studied had 2n = 22, which are new records, with two large blocks of doi.org/10.12705/625.16 CMA+ terminal bands and two smaller pericentromeric CMA+ bands Castro, J.P., Neto, E.M., Souza, L.G.R., Alves, L.I.F., Batista, (Table 2, Fig. 9). Pilosocereus includes 37 species, with 29 Brazilian F.R.C. & Felix, L.P. 2016. CMA band variability and physical native ones of which 26 are endemic to the country. Chromosome num- mapping of 5S and 45S rDNA sites in Brazilian Cactaceae: Per- bers were counted for five species and eight subspecies of the genus eskioideae and Opuntioideae. Brazil. J. Bot. 39: 613–620. Pilosocereus, with 2n = 22, except for P. catingicola subsp. catingicola, https://doi.org/10.1007/s40415-015-0248-5 P.catingicola subsp. salvadorensis and P. pachycladus subsp. pachycla- Guerra, M. 2000. Patterns of heterochromatin distribution in plant dus,where2n = 44 (Table 2, Figs. 11B,C, 12F) was counted, with two chromosomes. Genet. Molec. Biol. 23: 1029–1041. https://doi. heterochromatic terminal bands in diploid species and four in tetraploid. org/10.1590/S1415-47572000000400049 Curiously, diploid P. floccosus subsp. quadricostatus and P. tubercula- Guerra, M. 2005. Molecular cytogenetic characterization of parental tus, as well as tetraploid P. catingicola subsp. salvadorensis, differed genomes in the partial amphidiploid Triticum aestivum ×

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Thinopyrum ponticum. Genet. Molec. Biol. 28: 308–313. https:// in the gravel and scree slopes, 2941′04″N, 9830′04″E, 4290 m, doi.org/10.1590/S1415-47572005000200022 J.W. Zhang, Y.H. Zhang & Q.B. Gong 234 (KUN); China, Yunnan Guerra, M. & Souza, M.J. 2002. Como observar cromossomos – Province, Shangri-La County, in the alpine meadows, and scree Um guia de técnicas em citogenética vegetal, animal e humana. slopes, 2824′17″N, 9955′28″E, 4500 m, H. Sun SunH-07zx- Ribeirão Preto: FUNPEC. 2821 (KUN). Hernández-Hernández, T., Hernández, H.M., De-Nova, J.A., Our count confirms the diploid level for the species in agree- Puente, R., Eguiarte, L.E. & Magallón, S. 2011. Phylogenetic ment with Zhang & al. (2007, 2011). relationships and evolution of growth form in Cactaceae (Caryophyllales, Eudicotyledoneae). Amer. J. Bot. 98: 44–61. Saussurea obvallata (DC) Sch.Bip. https://doi.org/10.3732/ajb.1000129 **2n =2x = 16, CHN. China, Tibet, Riwoqê County, in the Stace, C.A. 2000. Cytology and cytogenetics as a fundamental taxo- alpine gravel and scree slopes, 3136′33″N, 9625′20″E, 4300 m, nomic resource for the 20th and 21st centuries. Taxon 49: J.W. Zhang & T.Y. Tu 122 (KUN). 451–477. https://doi.org/10.2307/1224344 We found the new chromosome number and ploidy level for this Stebbins, G.L. 1971. Chromosomal evolution in higher plants. species; previously, tetraploid level of S. obvallata was reported by London: Edward Arnold. Fujikawa (2004). Taylor, N. & Zappi, D. 2004. Cacti of eastern Brazil. Richmond: Royal Botanic Gardens, Kew. Saussurea velutina W.W.Sm. Taylor, N. & Zappi, D. 2008. Diversidade e endemismo das Cacta- *2n =2x = 30, CHN. China, Yunnan Province, Dêqên County, ceae na Cadeia do Espinhaço. Megadiversidade 4: 1–2. in the alpine screes, 2823′22″N, 9901′27″E, 4664 m, X.G. Ma & W.G. Sun 17-747 (KUN).

IAPT chromosome data 30/11 Soroseris erysimoides (Hand.-Mazz.) C.Shih **2n =4x = 32, CHN. China, Yunnan Province, Shangri-La Wen-Guang Sun, Hang Sun* & Zhi-Min Li* County, in the alpine scree slopes meadows and scree slopes, *Addresses for correspondence: Z.M. Li, [email protected]; 2831′16″N, 9957′05″E, 4620 m, J.W. Zhang, Y.H. Zhang & H. Sun, [email protected] Q.B. Gong 224 (KUN) [Fig. 14C]. This species was previously reported with diploid chromosome This work was supported by grants from the National Natural number of 2n = 16 by Zhang & al. (2007). Science Foundation of China (grant numbers 31670206, 31960046), Strategic Priority Research Program of Chinese Acad- Soroseris gillii (S.Moore) Stebbins emy of Sciences (grant number XDA 20050203), and NSFC- 2n =2x = 16, CHN. China, Tibet, Gonjo County, in the alpine Yunnan joint fund to support key projects (grant number meadows and scree slopes, 3022′13″N, 9829′44″E, 4300 m, J.W. U1802232). We would like to thank Dr. Xiang-Guang Ma, Zhang, Y.H. Zhang & Q.B. Gong 217 (KUN) [Fig. 14D]. Dr. Jian-Wen Zhang and Dr. Yong-Hong Zhang for their help during field research. Soroseris glomerata (Decne.) Stebbins 2n =4x = 32, CHN. China, Yunnan Province, Shangri-La * First chromosome count for the species. County, in the alpine screes, 2824′17″N9955′28″E, 4550 m, ** New chromosome number (cytotype) for the species. H. Sun SunH-07ZX-3365 (KUN) [Fig. 14E].

All materials fixed on seedlings produced from seeds collected Soroseris hirsuta (J.Anthony) C.Shih by us; identified by H. Sun and Z.M. Li; chromosomes were counted 2n =2x = 16, CHN. China, Sichuan Province, Kangding County, by W.G. Sun and Z.M. Li. in the alpine meadows, gravel and scree slopes, 2930′14″N, 10143′ 23″E, 4524 m, J.W. Zhang 026 (KUN) [Fig. 14F]. ASTERACEAE Crepis bodinieri H.Lév. Soroseris hookeriana Stebbins *2n =4x = 32, CHN. China, Yunnan Province, Kunming **2n =4x = 32, CHN. China, Sichuan Province, Xiangcheng County, in the forests, 2504′08″N, 10237′46″E, 2087 m, J.W. County, in the alpine scrubs and meadows, 2835′06″N, 9950′05″ Zhang 1043 (KUN) [Fig. 14A]. E, 4270 m, J.W. Zhang, B. Yang & H.L. Chen 934 (KUN) [Fig. 14G]. This species was reported previously with 2n = 16 by Zhang Parasyncalathium souliei (Franch.) J.W.Zhang, & al. (2007). We found a population of tetraploid level for this spe- Boufford & H.Sun cies. 2n =2x = 16, CHN. China, Sichuan Province, Daocheng County, in the alpine meadows, 2852′43″N, 10017′33″E, Stebbinsia umbrella (Franch.) Lipsch. 4625 m, J.W. Zhang, Y.H. Zhang & Q.B. Gong 264 (KUN) 2n =2x = 16, CHN. China, Yunnan Province, Shangri-La [Fig. 14B]; China, Sichuan Province, Baiyu County, in the scree County, in the alpine meadows, 2824′39″N9955′40″E, 4620 m, slopes, 3107′14″N, 9922′01″E, 3876 m, J.W. Zhang, Y.H. Zhang H. Sun SunH-07ZX-3421 (KUN); China, Sichuan Province, Xiang- & Q.B. Gong 180 (KUN); China, Tibet, Gonjo County, in the alpine cheng County, in the alpine meadows and scree slopes, 2852′44″ meadows and scree slopes, 3131′02″N, 9830′13″E, 4075 m, J.W. N, 10017′30″E, 4607 m, J.W. Zhang, B. Yang & H.L. Chen 931 Zhang, Y.H. Zhang & Q.B. Gong 215 (KUN); China, Tibet, Zayü (KUN) [Fig. 14H]; China, Sichuan Province, Jiulong County, in the County, in the scree slopes, 2919′24″N, 9702′06″E, 4700 m, alpine meadows and scree slopes, 2917′20″N, 10130′50″E, H.L. Chen & J.P. Yue 003 (KUN); China, Tibet, Markam County, 4473 m, J.W. Zhang, Y.H. Zhang & Q.B. Gong 019 (KUN).

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Syncalathium chrysocephalum (C.Shih) C.Shih slopes, 3213′45″N, 10236′03″E, 4215 m, J.W. Zhang, W.G. Sun 2n =2x = 16, CHN. China, Tibet, Dêngqên County, in the alpine & L.S. Qian 466 (KUN); China, Sichuan Province, Xiaojin County, meadows and scree slopes, 3134′41″N, 9535′44″E, 4848 m, J.W. in the alpine grasslands and scree slopes, 3213′45″N, 10236′03″E, Zhang, W.G. Sun & L.S. Qian 193 (KUN); China, Tibet, Riwoqê County, 4215 m, J.W. Zhang, W.G. Sun & L.S. Qian 530 (KUN). in the alpine meadows, gravel and scree slopes, 3136′37″N, 9625′12″E, 4638 m, J.W. Zhang, W.G. Sun & L.S. Qian 217 (KUN) [Fig. 14I]. Syncalathium kawaguchii (Kitam.) Y.Ling 2n =2x = 16, CHN. China, Tibet, Lhünzhub County, in the Syncalathium disciforme (Mattf.) Y.Ling alpine meadows, 3006′36″N, 9116′08″E, 4848 m, J.W. Zhang 2n =2x = 16, CHN. China, Qinhai Province, Chindu County, in 3431 (KUN); China, Tibet, Damxung County, in the alpine slopes, the alpine grasslands, gravel and scree slopes, 3309′18″N, 9716′ 3042′03″N, 9105′15″E, 4848 m, J.W. Zhang 3435 (KUN) 36″E, 4548 m, J.W. Zhang, W.G. Sun & L.S. Qian 309 (KUN); [Fig. 14K]; China, Tibet, Dêngqên County, in the alpine slopes, China, Qinhai Province, Madu County, in the alpine meadows, gravel 3016′03″N, 9639′19″E, 4848 m, J.W. Zhang 3366 (KUN); China, and scree slopes, 3407′12″N, 9739′28″E, 5029 m, J.W. Zhang, Tibet, Riwoqê County, in the alpine slopes, 3106′23″N, 9630′23″E, W.G. Sun & L.S. Qian 327 (KUN) [Fig. 14J]; China, Sichuan Prov- 4525 m, J.W. Zhang 3305 (KUN); China, Tibet, Gongbo’gyamda ince, Heishui County, in the alpine grasslands, gravel and scree County, in the alpine slopes and gravelly areas on river beaches,

Fig. 14. Photographs of mitotic metaphase chromosomes of 16 taxa from China. A, Crepis bodinieri,2n = 32; B, Parasyncalathium souliei,2n = 16; C, Soroseris erysimoides,2n = 32; D, S. gillii,2n = 16; E, S. glomerata,2n = 32; F, S. hirsuta,2n = 16; G, S. hookeriana,2n = 32; H, Stebbinsia umbrella,2n = 16; I, Syncalathium chrysocephalum,2n = 16; J, S. disciforme,2n = 16; K, S. kawaguchii,2n = 16; L, Youngia paleacea,2n = 16; M, Y. simulatrix,2n = 16; N, Draba oreodoxa,2n = 24; O, Salvia flava,2n = 16; P, S. przewalskii,2n = 32. — Scale bars = 5 μm.

E28 Version of Record TAXON 68 (5) • October 2019: E1–E29 Marhold & Kucˇera (eds.) • IAPT chromosome data 30

2950′49″N, 9221′13″E, 4786 m, J.W. Zhang 4081 (KUN); China, IAPT chromosome data 30/12 Tibet, Lhari County, in the alpine scree slopes, 3057′08″N, 9238′ 27″E, 4750 m, J.W. Zhang 3425 (KUN); China, Tibet, Biru County, Mariya S. Voronkova, Evgeny V.Banaev,* Mariya A. Tomoshevich in the alpine scree slopes, 3154′58″N, 9324′06″E, 4650 m, J.W. & Vera A. Kostikova Zhang 3412 (KUN). *Address for correspondence: [email protected]

Youngia paleacea (Diels) Babc. & Stebbins * First chromosome count from the given region. 2n =2x = 16, CHN. China, Yunnan Province, Shangri-La County, on the slope of valley, 2721′53″N9954′17″E, 3122 m, POLYGONACEAE H.L. Chen & J.P. Yue 048 (KUN) [Fig. 14L]. Atraphaxis frutescens (L.) K.Koch *2n = 16, 18, 20, 22, CHN. Russia, Republic of Tuva, Youngia simulatrix (Babc.) Babc. & Stebbins Ulug-Khemskii Raion, vicinity of Khayyrakan village, steppe, *2n = 16, CHN. China, Tibet, Damxung County, in the alpine 5133.776′N, 9303.716′E, 01 Aug 2015, D.K. Kostikov & V.A. scree slopes, 3046′32″N, 9051′06″E, 4723 m, J.W. Zhang 3436 Kostikova 3000905 (NSK). (KUN) [Fig. 14M]. *2n = 16, 32, 56, CHN. Russia, Novosibirskaya Oblast’, Ordynskii Raion, vicinity of Antonovo village, bank of the Ob River, BRASSICACEAE sandy scree, southern slope, 5405.501′N, 8124.945′E, 11 Jul 2013, Draba oreodoxa W.W.Sm. D.K. Kostikov & V.A. Kostikova 3000901 (NSK). *2n =2x = 24, CHN. China, Yunnan Province, Shangri-La *2n = 18, 22, CHN. Russia, Republic of Tuva, Barun- County, in the alpine screes, 2834′14″N9948′24″E, 4317 m, J.P. Khemchiksiy Raion, vicinity of Shekpeer village, near dry river, Yue 053 (KUN) [Fig. 14N]. sands, pebbles, steppe, 5109.712′N, 9040.322′E, 03 Aug 2015, D.K. Kostikov & V.A. Kostikova 3000906 (NSK). LAMIACEAE *2n = 22, CHN. Russia, Republic of Khakasiya, Ust’- Salvia flava Forrest ex Diels Abakanskii Raion, vicinity of Kamyshovaya village, lake Ulug-Kol 2n =2x = 16, CHN. China, Sichuan Province, Xiangcheng (bitter-salty), steppe, 5347.871′N, 9042.027′E, 06 Aug 2015, County, roadside, 2835′47″N, 9950′23″E, 3872 m, W.G. Sun D.K. Kostikov & V.A. Kostikova 3000904 (NSK). 2237 (KUN) [Fig. 14O]. *2n = 48, 50, 52, CHN. Russia, Omskaya Oblast’, Kalachinskii Raion, Kalachinsk, right bank of the Om River, sandy clayey south- Salvia przewalskii Maxim. ern slope, 5503.737′N, 7436.398′E, 05 Aug 2014, D.K. Kostikov 2n =4x = 32, CHN. China, Yunnan Province, Shangri-La & V.A. Kostikova 3000902 (NSK). County, roadside, 2754′29″N9938′07″E, 3890 m, W.G. Sun *2n = 48, 64, 80, CHN. Russia, Altaiskii Krai, Slavgorodskii 37151 (KUN) [Fig. 14P]. Raion, south-eastern slope of the shore of Lake Bol’shoe Yarovoe (bitter-salty), steppe. 5252.887′N, 7832.842′E, 14 Aug 2015, LITERATURE CITED D.K. Kostikov & V.A. Kostikova 3000911 (NSK). Fujikawa, K., Ikeda, H., Murata, K., Kobayashi, T., Nakano, T., *2n = 58, 62, 64, CHN. Russia, Altaiskii Krai, Burlinskii Raion, Ohba, H. & Wu, S.G. 2004. Chromosome numbers of fifteen vicinity of Petrovka village, the shore of the lake Bol’shoe Topol’noe species of the genus Saussurea DC. (Asteraceae) in the Hima- (bitter-salty); steppe, 5321.754′N, 7802.758′E, 13 Jul 2013, D.K. layas and the adjacent regions. J. Jap. Bot. 79: 271–280. Kostikov & V.A. Kostikova 3000903 (NSK). Zhang, J.W., Sun, H. & Nie, Z.L. 2007. Karyological studies on the Sino-Himalayan endemic Soroseris and two related genera of Atraphaxis pungens (M.Bieb.) Jaub. & Spach tribe Lactuceae (Asteraceae). Bot. J. Linn. Soc. 154: 79–87. *2n = 18, 20, CHN. Russia, Republic of Tuva, Pii-Khemskii https://doi.org/10.1111/j.1095-8339.2007.00644.x Raion, vicinity of Shivilig village, a large rocky southern slope, Zhang, J.W., Boufford, D.E. & Sun, H. 2011. Parasyncalathium 5213.434′N, 9344.148′E, 26 Jul 2015, D.K. Kostikov & V.A. J.W. Zhang, Boufford & H.Sun (Asteraceae, Cichorieae): A Kostikova 3000908 (NSK). new genus endemic to the Himalaya-Hengduan Mountains. *2n = 18, 20, 22, CHN. Russia, Republic of Tuva, Kyzylskii Taxon 60: 1678–1684. https://doi.org/10.1002/tax.606012 Raion, vicinity of Kara-Khaak village, steppe, 5152.955′N, 9428.657′E, 27 Jul 2015, D.K. Kostikov & V.A. Kostikova 3000909 (NSK). *2n = 42, 44, 48, 52, 62, 64, 66, 68, CHN. Russia, Republic of Altai, Kosh-Agachskii Raion, vicinity of Kokorya village, the right bank of the Chuya River, the southern slope of the hills, the desert steppe, 4355′41.82″N, 7619′28.20″E, 02 Aug 2013, D.K. Kostikov & V.A. Kostikova 3000910 (NSK). *2n = 62, 66, 68, 72, CHN. Russia, Republic of Tuva, Chaa-Khol’skii Raion, the shore of the Sayano- Shushenskii reservoir, steppe southern rocky slope, 5137.07′N, 9225.472′E, 12 Jul 2014, D.K. Kostikov & V.A. Kostikova 3000907 (NSK).

Version of Record E29