DIATOM ALGAE (BACILLARIOPHYTA) IN WATERBODIES AND WATERCOURSES ON ( ARCHIPELAGO) S.I. Genkala,#, V.A. Gabyshevb,## aInstitute for Biology of Inland Waters of Russian Academy of Sciences Borok, Nekouz District, Yaroslavl Region, 152742, #e-mail: [email protected] bInstitute for Biological Problems of Cryolithozone of Siberian Branch of Russian Academy of Sciences Lenin Ave., 41, Yakutsk, 677980, Russia ##e-mail: [email protected] DOI: 10.31857/S0006813620080049 This electron microscopy study of phytoplankton from 12 waterbodies and watercourses on Kotelny Island (New Siberian Islands) has revealed 70 taxa of diatom algae from 28 genera, including 19 forms from 9 genera identified only to the genus. The study has significantly expanded the taxonomic spectrum of Bacillariophyta of the island both at the species (from 22 to 71) and genus (from 7 to 31) levels. Among the recorded species, 34 are new for the flora of New Siberian Islands and 22 for the flora of Yakutia (the Republic of ). The majority of the species new for the Republic are rare freshwater species preferring oligotrophic waterbodies. Keywords: Kotelny Island, Yakutiya, phytoplankton, Bacillariophyta, electron microscopy, new for flora.

Acknowledgements This study was conducted within the framework of the state assignment projects “Fundamental and applied aspects of the study on diversity of the plant world of North and Central Yakutia” (№ АААА-А17-117020110056-0) and “Systematics, diversity and phylogeny of aquatic autotrophic organisms in Russia and other regions of the world” (№ АААА-А18- 118012690095-4). The authors are grateful to Doctor of Biological Science, Professor E.V. Likhoshvai for the opportunity to use equipment of the Shared Use Center “Electron Microscopy” in Limnological Institute of the Siberian Brunch of the Russian Academy of Sciences; Chief Specialist at the Shared Use Center “Ultramicroanalysis” V.I. Egorov, and Research Engineer of the Educational, Scientific and Technological Laboratory “Graphene Nanotechnology” of the North-Eastern Federal University V.B. Timofeev for their help with SEM and TEM.

References

1 Anisimova M.A., Ivanova V.V., Pushina Z.V., Pitulko V.V. 2009. Lagunnue otlozheniya ostrova Zhokhova: vozrast, usloviya formorovaniya i znachenie dlya paleogeograficheskikh rekonstrultsiy regiona Novosibirskikh ostrovov [Zhokhov Island Lagoon Deposits, Their Age, Formation and Significance to Paleo-geographic Reconstruction of the New Siberian Islands Region]. – Izvestiya RAN. Seria geograficheskaya. P. 107–119 (In Russ.). Bondarenko N.A., Genkal S.I. 2005. On the records of lake Baikal endemic algae in mountain lakes of Zabaikalye. – Botanicheskii zhurnal. 90(9): 1389–1401 (In Russ.). Diatomovyye vodorosli SSSR (iskopayemyye i sovremennyye). T. 1. [Diatoms of the USSR (fossil and recent). Vol. 1]. 1974. Leningrad. 403 p. (In Russ.). Genkal S.I., Chekryzheva T.A, Komulaynen S.F. 2015. Diatom algae in waterbodies and watercourses of Karelia. Мoscow. 202 p. (In Russ.). Gabyshev V.A., Gabysheva O.I. 2018. Fitoplankton krupnykh rek Yakutii i sopredel'nykh territoriy Vostochnoy Sibiri [Phytoplankton of the largest rivers of Yakutia and adjacent territories of Eastern Siberia]. Novosibirsk. 414 p. (In Russ.).

Genkal S.I., Bondarenko N.A., Shur L.A. 2011. Diatomovye vodorosli ozer yuga i severa Vostochnoi Sibiri [Diatoms of lakes from south and north part of Eastern Siberia]. Rybinsk. 72 p. (In Russ.). Genkal S.I., Gabyshev V.A. 2018. New records of centric diatoms from Yakutia (Bolshoe Toko Lake): SEM morphology, ecology and distribution. – Novosti Sist. Nizsh. Rast. 52(2): 245– 252. https://doi.org/10.31111/nsnr/2018.52.2.245 Genkal S., Gabyshev V., Kulikovskiy M., Kuznetsova I. 2018. Pliocaenicus bolshetokoensis – a new species from Lake Bolschoe Toko (Yakutia, Eastern Siberia, Russia). – Diatom Research. 33(2): 145–153. https://doi.org/10.1080/0269249X.2018.1477690 Genkal S.I., Trifonova I.S. 2009. Diatom algae of the plankton of Lake Ladoga and water- bodies of its basin. Rybinsk. 72 p. (In Russ.). Genkal S.I., Vekhov N.V. 2007. Diatomovye vodorosli vodoemov Russkoy Arktiki: arkhipelag Novaya Zemlya i ostrov Vaygach [Diatom algae of water bodies in the Russian Arctic: Novaya Zemlya Archipelago and Vaigach island]. Moscow. 64 p. (In Russ.). Genkal S.I., Yarushina M.I. 2018. Diatom algae of poorly studied aquatic ecosystem in the Far North of Western Siberia. Мoscow. 212 p. Gorodkov B.N. 1956. Rastitelnost’ i pochvy o. Kotelnyi [Vegetation and Soils of Kotelny Island]. Issue 2. Moscow, Leningrad. P. 7–132 (In Russ.). Karayeva N.I., Genkal S.I. 1993. The diatom of the genus Navicula Bory (Bacillariophyta) in the Volga River. – Limnologica. 23(4): 309–321. Kharitonov V.G. 2014. Diatoms of . Magadan. 496 p. (In Russ.).

2 Kharitonov V.G., Genkal S.I. 2012. Diatoms of the Elgygytgyn Lake and its vicinities (Chukotka). Маgadan. 402 p. (In Russ.). Klimat Yakutskoy ASSR (atlas) 1968. [Climate of Yakut ASSR (atlas)]. Leningrad. 33 p. (In Russ.). Korneva L.G. 2015. Phytoplankton of Volga River basin reservoirs. Kostroma. 284 p. (In Russ.). Kosinskaya E.K. 1936. Desmidievye vodorosli iz Arktiki [Desmids from the Arctic]. – Trudy Botanicheskogo instituta AN SSSR. Ser. II. 3: 401–440 (In Russ.). Kosinskaya E.K.1956. K flore presnovodnykh vodorosley Novosibirskikh ostrovov [To the Flora of Freshwater Algae of the New Siberian Islands]. – Trudy Botanicheskogo instituta AN SSSR. Ser. II. 10: 5–32 (In Russ.). Krammer K. 1997a. Die cymbelloiden Diatomeen. Teil 1. Allgemeines und Encyonema part. – Bibliotheca Diatomologica. 36: 1–382. Krammer K. 1997b. Die cymbelloiden Diatomeen. Teil 2. Encyonema part., Encyonopsis und Cymbellopsis. – Bibliotheca Diatomologica. 7: 1–469. Krammer K. 2000. Pinnularia. – In: Diatoms of Europe. Vol. 1. P. 1–703. Krammer K. 2002. Cymbella. – In: Diatoms of Europe. Vol. 3. P. 1–584. Krammer K. 2003. Cymbopleura, Delicata, Navicymbula, Gomphocymbellopsis, Afrocymbella. – In: Diatoms of Europe. Vol. 4. P. 1–530. Krammer K., Lange-Bertalot H. 1986. Bacillariophyceae. Teil 1. Naviculaceae. – In: Die Süsswasserflora von Mitteleuropa. Bd 2/1. Stuttgart. S. 1–876. Krammer K., Lange-Bertalot H. 1988. Bacillariophyceae. Teil 2. Epithemiaceae, Bacillariaceae, Surirellaceae. Bd. 2/2. – In: Die Süsswasserflora von Mitteleuropa. Stuttgart. S. 1– 536. Krammer K., Lange-Bertalot H. 1991a. Bacillariophyceae. Teil 3: Centrales, Fragilariaceae, Eunotiaceae. Bd. 2/3. – In: Die Süsswasserflora von Mitteleuropa. Stuttgart, New York. S. 1–576. Krammer K., Lange-Bertalot H. 1991b. Bacillariophyceae. Teil. 4. Achnanthaceae, Kritische Erganzungen zu Navicula (Lineolatae) und Gomphonema. Bd. 2/4. – In: Die Süsswasserflora von Mitteleuropa. Stuttgar. P. 1–437. Kudryavtsev V.A., Dostovalov V.N. 1967. Obshchee merzlotovedenie [General permafrost]. Мoscow. 403 p. (In Russ.). Kulikovskiy M.S., Glushchenko A.M., Genkal S.I., Kuznetsova I.V. 2016. Identification book of diatoms from Russua. Yaroslavl. 804 p. (In Russ.).

3 Lange-Bertalot H. 2001. Navicula sensu stricto, 10 genera separated from Navicula sensu lato Frustulia. – In: Diatoms of Europe. Vol. 2. P. 1–526. Lange-Bertalot H., Genkal S. I. 1999. Diatoms of Siberia. I. – Iconographia Diatomologia. 6: 7–272. Lange-Bertalot H., Bak M., Witkowski A. 2011. Eunotia and some related genera. – In: Diatoms of Europe. Vol. 6. P. 1–747. Lange-Bertalot H.,Hofmann G., Werum M., Cantonati M. 2017. Freshwater benthic diatoms of Central Europe. Schmitten-Oberreifenberg. 942 p. Lange-Bertalot H., Moser G. 1994. Brachysira-Monographie der Gattung. Wichtige Indicator-species für das Gewässer-monitoring und Naviculadicta nov. gen. Ein Lösungsvorschlag zu dem Problem Navicula sensu lato onhe Navicula sensu strict. – Bibliotheca Diatomologica. 29: 1 – 212. Levkov Z. 2009. Amphora sensu lato. – In: Diatoms of Europe. Vol. 5. P. 1–916. Levkov Z. Mitić-Kopanja D., Reichardt E. 2016. The diatom genus Gomphonema from the Republik of Macedonia. – In: Diatoms of Europe. Vol. 8. P. 1–552. Levkov Z., Metzeltin D., Pavlov A. 2013. Luticola, Luticolopsis. – In: Diatoms of Europe. Vol. 7. P. 1–697. Loseva E.I., Stenins A.S., Marchenko-Vagapova T.I. 2004. Cadastre of the fossil and recent diatoms from northeastern Europe. Syktyvkar.160 p. (In Russ.). Opredelitel’ presnovodnykh vodorosley SSSR. Vyp. 4. Diatomovye vodorosli. 1951. [Key to freshwater algae of the USSR. Diatom algae. Issue 4]. Мoscow. 619 p. (In Russ.). Palagushkina O.V., Wetterich S., Schirrmeister L., Nazarova L.B. 2017. Modern and Fossil Diatom Assemblages from Bol’shoy Lyakhovsky Island (New Siberian Archipelago, Arctic Siberia). – Contemporary Problems of Ecology 10(4): 380–394. https://doi.org/10.1134/S1995425517040060 Popovskaya G.I., Genkal S.I., Likhoshway Ye.V. 2016. Diatoms of the Plankton of Lake Baikal: Atlas and Key. Novosibirsk. 180 p. Potapova M.G., Hamilton P.B., Kopyrina L.I., Sosina N.K. 2014. New and rare diatom (Bacillariophyta) species from a mountain lake in Eastern Siberia. – Phytotaxa 156(3): 100–116. http://dx.doi.org/10.11646/phytotaxa.156.3.2 Reichardt E. 1999. Zur Revision der Gattung Gomphonema. – Iconographia Diatomologica. 8: 1–203. Ushnitskaya L.A., Gorodnichev R.M., Spiridonova I.M., Pestryakova L.A. 2013. Predvaritelnaya limnologocheskaya kharakteristika vodoemov poluostrova Faddeevskiy (Novosibirslie ostrova. [Preliminary limnological characteristics of waterbodies on the Fadeevsky

4 Island (New Siberian Islands)]. – Mezhdunarodnyy zhurnal prikladnykh issledovaniy. 8–2: 189– 192 (In Russ.). Vasil’eva I.I. 1986. Vodorosli Novosibirskikh ostrovov [Algae of the New Siberian Islands]. – In: Tez. dokl. XI Vsesoyuzn. simp. «Biologicheskie problemy Severa [Biological problems of the North]. Vyp. 2. Yakutsk. P. 39–40 (In Russ.). Zakharova V.I., Kuznetsova L.V., Ivanova E.I., Vasil’eva-Kralina I.I., Gabyshev V.A., Egorova A.A., Zolotov V.I., Ivanova A.P., Ignatov M.S., Ignatova E.A., Kopyrina L.I., Krivoshapkin K.K., Mikhaleva L.G., Pestryakova L.A., Poryadina L.N., Pshennikova E.V.,

Pemigailo P.A., Sosina N.K., Sofronova E.V. 2005. Raznoobrazie rastitel’nogo mira Yakutii [Diversity of the Vegetable Kingdom in Yakutia]. Novosibirsk. 328 p.

5