2-6 May Barcelona, Spain2-6 2-6

ISEV2018 I SEV20 18 FINALABSTRA PROGRAMCT BOOK ANNUALP a lau deME C o ng re s sos de BETING arce lona - Fira M o nt juic

ANNUALMEETING ANNUALMEETING 2-6 May Barcelona, Spain2-6 2-6

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 1 1 Table of Contents

Letter from the ISEV President 3

Letter from the ISEV IOC Chair 4

Executive Board and Board of Directors 5

International Organizing Committee 6

Palau de Congressos Barcelona – Fira Montjuic Floor Plan 7

General Information 9

Plenary Speakers 11

Program Information 15

Satellite EVents 35

Abstract Directory 38

Sponsor Directory 54

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 2 Welcome from ISEV President

Dear Colleagues,

On behalf of the International Society for Extracellular Vesicles (ISEV) Board of Directors and the International Organising Committee (IOC), it is a pleasure to welcome you to ISEV2018. This year, ISEV has teamed up with GEIVEX (Spanish Society for Innovation and Investigation in Extracellular Vesicles, www. geivex.org) to organize ISEV2018. The ISEV2018 IOC, composed of ISEV and GEIVEX board members, had worked hard to prepare an interesting and dynamic program that includes educational sessions, along with the latest advances of the field - representing both fundamental and clinical aspects.

In 2018, we will host a record number of attendees at the annual ISEV meeting and this reflects the growing recognition of the field and the numbers of researchers now working on extracellular vesicles.

ISEV2018 begins with the Education Day, covering two tracks in “Biology and Technology” and “EVs in Health and Disease”. The main meeting features again this year the ‘Meet the Experts’ and ‘Experts Workshops’ sessions in addition to the national societies session – all aimed at increasing networking amongst our international community. There are satellite sessions covering “Emerging Trends in HIV, NeuroAIDS, Drug Abuse and EVs”, an “exRNA ERCC Workshop”, and a meeting of the ISEV-ISAC-ISTH EV flow cytometry workgroup. This year we introduce sessions including an Early Career Development Session on Academia and Industry in the field of EVs as well as a “Meet the Editors” session featuring presentations from journal editors relevant to the EV field.

I would like to thank the members of the IOC, led by Juan M. Falcón-Pérez, for their efforts in making ISEV2018 possible. Also, thanks to the ISEV Board of Directors for their continued commitments to growing and supporting ISEV and serving the community of EV researchers. On behalf of ISEV, we also acknowledge and thank our corporate sponsors who provide essential support for the meeting.

Don’t forget to attend the not-to-be-missed Networking Event on Saturday 5th May at 20:15 – a great opportunity to network and socialize with attendees of the meeting.

I hope you enjoy all that ISEV2018 has to offer and wish you all the best with your EV research that follows!

Regards,

Andy Hill, PhD President, ISEV

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 3 Welcome from the International Organizing Committee Chair

Dear Colleagues:

On behalf of the International Organizing Committee, it is with great pleasure that I welcome you to the the seventh Annual Meeting of the International Society for Extracellular Vesicles. It has been an honor to organize this meeting where scientists from around the globe share the latest knowledge on extracellular vesicles that represent a new paradigm both in cell biology and medicine. They are considered to offer unprecedented opportunities for future applications from new generation diagnostic or prognostic biomarkers to therapeutic applications. The International Society for Extracellular Vesicles (ISEV) was founded in 2011, and as the authoritative international community for extracellular vesicle research, it focuses on all aspects of extracellular vesicle investigations.

More than 1000 delegates will attend ISEV2018 presenting over 700 scientific presentations. The annual meeting will feature speakers covering novel and stimulating scientific areas including “Autophagy, Metabolism, Aging”, “Advances in Exosome Biology”, “Environment, Diet & Health”, “Neglected Diseases” and “Tissue Architecture”. A dedicated scientific program with 30 symposium sessions will cover all aspects of EV research frombasic research, technological development and clinic applications. The program will be completed by the Meet- the-Expert sessions on “EV-Biobanks”, “EV-Regulatory aspects”, “EV-RNA”, “EV-Biomarkers”, “EV-Immunology”, “EV-Neglected diseases”, “EV-Leukemia (Josep Carreras Foundation)”, “EV-in vivo Imaging”, and the “EV-blood roadmap”. In addition, there will be satellite sessions on Flow Cytometry (joint session with ISAC and ISTH), EV- HIV and RNA-ERCC, as well as, the meeting with the EV-related National Societies. This year also offers sessions to meet the editors of journals, and have the opportunity to listen tips from young EV-successful researchers on academic an industry tracks during the “Young EV Career Development” session. During the congress, ISEV will introduce new developments in EV-based 3D animation and outreach strategies that ISEV is supporting.

Be sure to attend the Opening Ceremony and enjoy a performance by tenor Sergi Gimenez Carreras. The networking event on Saturday evening will be held in Cupula at the Arenas de Barcelona with different entertainment options embedded in the cosmopolitan Barcelona´s environment.

ISEV is committed to creating a most memorable scientific congress. Through your participation, interest, and support, you will certainly contribute to the success of the meeting and the advancement of novel extracellular vesicle studies.

Again, welcome to Barcelona!

On behalf of the International Organizing Committee,

Juan Falcón-Pérez Chair, International Organizing Committee

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 4 About the International Society for Extracellular Vesicles

ISEV is a global society of researchers and scientists studying exosomes and microvesicles. With over 1,000 members, ISEV is the leading advocate and guide of extracellular vesicle research with a mission of advancing extracellular vesicle research globally. ISEV Executive Board and Board of Directors 2016-2018

President Executive Chair, Communications

Carolina Soekmadji Andrew Hill Dolores Di Vizio Australia Australia United States

Secretary General Past President

Hidetoshi Tahara Asia Pacific Kenneth Witwer Jan Lötvall United States Sweden

Treasurer Chair International Organizing Committee Ana Claudia Torrecilhas Brazil

Chris Gardiner United Kingdom Juan Falcón-Pérez Spain Executive Chair, Science/Meetings Alissa Weaver Members at Large United States

Marca Wauben The Netherlands Rienk Nieuwland Executive Chair, Education Hubert Yin The Netherlands China/United States

Edit Buzas Hungary Susmita Sahoo Lei Zheng United States China

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 5 2018 International Organizing Committee Members

Juan Falcón-Pérez, PhD Isabel Guerrero Hidetoshi Tahara IOC Chair UAM/CBM-SO Hiroshima University CIC bioGUNE Madrid, Spain Hiroshima, Japan Bizkaia, Spain

Andrew Hill Marca Wauben Hernando A. del Portillo ISEV President Utrecht University GEIVEX President La Trobe University Utrecht, The Netherlands ICREA at ISGlobal and IGTP Melbourne Australia Barcelona, Spain

Kenneth Witwer Antonio Marcilla Johns Hopkins University Francesc Borrás Universitat de València Baltimore, MD USA "Germans Trias i Pujol" Research Institute Valencia, Spain (IGTP),Can Ruti Campus Barcelona, Spain

María Yáñez-Mó Héctor Peinado UAM/CBM-SO Spanish National Cancer Madrid, Spain Edit Buzas Research Center – CNIO Semmelweis University Madrid, Spain Budapest, Hungary

Hubert Yin Colorado University Susmita Sahoo Boulder, Colorado Dolores DiVizio Mount Sinai School of Medicine Cedars-Sinai Medical Center New York, NY USA Los Angeles, CA USA

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 6 Hall 5 Montjuïc ROOM 9 41 sqm LEVEL 4 OFFICES 16 sqm each (X2) Auditorium ROOM 7 ROOM 5 1362 sqm 277 sqm Palau deHall Congressos 5 ROOM Barcelona 8 –Hall Fira 5 Montjuic 150 sqm ROOM 3 Floor PlanLEVEL 3 Montjuïc ROOM 6 183 sqm Halls and open areas ROOM 9 310 sqm Enclosed rooms ROOMS 1 and 2 41 sqm LEVEL 4 39 sqm each Elevators OFFICES Restrooms 16 sqm each (X2) Auditorium ROOM 7 ROOM 5 Catering outlet ROOM 4 Hall 5 1362 sqm 277 sqm Desk/ cloakroom 108 sqm Hall 5 ROOM 8 LEVEL 2 Montjuïc 150 sqm ROOM 3 VIP AREA ROOM 9 Public Access LEVEL 3 183 sqm 341 sqm 41 sqm ROOM 6 Halls and open areas 528 528 sqm LEVEL 4 310 sqm height 5,69m ROOMS 1 and 2 Enclosed rooms OFFICES 39 sqm each Elevators 16 sqm each (X2) Auditorium ROOM 7 Restrooms ROOM 5 Public Access 1362 sqm LEVEL 1 Catering outlet 277 sqm ROOM 4 Hall 5 ROOM 8 street level Desk/ cloakroom 108 sqm 150 sqm 2,28 sqm ROOM 3 LEVELheight 2 5,36m LEVEL 3 1,95 sqm ROOM 6 183 sqm VIP AREA height 2,0m Public Access Halls and open areas 310 sqm 341 sqm Enclosed rooms ROOMS 1 and 2 528 528 sqm height 5,69m 39 sqm each ROOMS 11 and12 Elevators 124 and 138 sqm Restrooms LEVEL 0 Public Access Catering outlet ROOM 4 LEVEL 1 Desk/ cloakroom 108 sqm street level 2,28 sqm LEVEL 2 height 5,36m 1,95 sqm VIP AREA Public Access height 2,0m 341 sqm 528 528 sqm height 5,69m ROOMS 11 and12 124 and 138 sqm Public Access LEVEL 0 LEVEL 1 street level 2,28 sqm height 5,36m 1,95 sqm height 2,0m

ROOMS 11 and12 124 and 138 sqm LEVEL 0

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 7 Acknowledgements ISEV gratefully acknowledges the support of the 2018 Annual Conference from our Sponsors: PLATINUM

SILVER

BRONZE

LANYARD SPONSOR NOTEPAD SPONSOR

EXHIBITORS

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 8 General Information

Abstracts Exhibits Abstracts for plenary and parallel sessions are Educational and informational exhibits will be included in the Abstract Book, which is available available for viewing during the Annual Meeting for download from the conference website: and representatives will be on hand to answer http://www.isev.org/page/ISEV2018homepage. questions. Please visit our sponsors and their exhibits, on Level 1 Foyer, as they are an integral part of the meeting. ISEV General Assembly Thursday, 3 May 08:30 - 13:30; 15:00 - 18:30 Members are strongly encouraged to attend Friday, 4 May 10:00 - 13:30; 15:00 - 18:30 the General Assembly on Saturday, 5 May, from Saturday, 5 May 10:00 - 13:30; 15:00 - 18:30 11:35 -12:30 in the Auditorium. Sunday, 6 May 08:00 - 10:30 Conference Registration / Meeting App Information Desk ISEV is pleased to announce the 2018 Annual The ISEV Annual Meeting Registration/Information Meeting App! The App is exclusively available Desk, located on Level 1 Foyer, will be open and for registered attendees. To add the App to staffed as follows: your mobile device, download “eventScribe” from Tuesday, 1 May 16:30 – 18:30 itunes or google play. Launch, click search, type ISEV2018, click on the ISEV icon to launch the Wednesday, 2 May 07:30 – 19:30 ISEV app. Click create account and enter ISEV2018 Thursday, 3 May 07:30 – 20:00 as the event code to create an account and log in. Friday, 4 May 07:30 – 20:00 Saturday, 5 May 07:30 – 20:00 Name Badges Sunday, 6 May 08:00 – 14:00 Your badge serves as your admission to all meeting events. During the events, you name badge must be worn at all times. For security Evaluations reasons, persons without badges will not be During the ISEV Annual Meeting, at the end permitted to attend events. of every day, you will receive an email link(s) to complete a daily evaluation. Photography Shortly after the meeting, ISEV will email a link Any photography, filming, taping, recording or to the evaluation form. ISEV values your feedback reproduction in any medium including the use of and you are strongly encouraged to complete tripod-based equipment of any of the programs the evaluations as this will assist in developing and/or posters presented at the Annual Meeting future conferences. without the express written consent of ISEV is strictly prohibited. Exceptions to this policy include non-flash photography and audiotape recording - using hand-held equipment for strictly personal use, are permitted if not disruptive.

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 9 General Information

Poster Presentations ISEV invites you to meet the authors of the accepted posters during the scheduled poster sessions which will be held on Level 1 Foyer of the convention center.

Authors/Presenters’ please note: ISEV will not be responsible for removing and/or returning posters. All posters not removed by 19:30 on the day of presentation will be disposed of.

Social Programme You are cordially invited to join the ISEV Board of Directors, International Organizing Committee, and fellow delegates at the following event: Networking Event Saturday, 5 May 20:15 – 23:59 Cupula at the Arenas de Barcelona The ISEV2018 Networking Event provides a great opportunity to meet the leaders and young researchers in the field of Extracellular Vesicles while both strengthening existing and initiating new collaborations. Expand your circle of colleagues and discover the latest news and insights into the research of your peers! This event will also feature, food stations, beverages, and great music!

Social Media Find out what is happening live at the meeting by following the ISEV2018 twitter team @ISEV.org. We want to hear from you so join in on the conversation by tagging your tweets with #ISEV2018. If you are sitting in one session and wondering what you’re missing in another why not tweet about it?

Speaker Ready Room To avoid any delays in presentations, speakers and presenters are asked to visit the Speaker Ready Room at least two hours prior to their presentation. The Speaker Ready Room is located in Room 8 on the Third Level. Audio-visual assistance will be available.

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 10 Plenary Speakers

ISEV is honored to present the list of esteemed speakers:

Mina J. Bissell, PhD Lawrence Berkeley National Laboratory Berkeley, CA United States

Mina J Bissell is Distinguished Scientist, the highest rank bestowed at Lawrence Berkeley National Laboratory (LBNL) and serves as Senior Advisor to the Laboratory Director on Biology. She is also Faculty of four Graduate Groups in UC Berkeley: Comparative Biochemistry, Endocrinology, Molecular Toxicology, and Bioengineering (UCSF/UCB joint program). Having challenged several established paradigms, Bissell is a pioneer in breast cancer research and her body of work has provided much impetus for the current recognition of the significant role that extracellular matrix (ECM) signaling and microenvironment play in gene expression regulation in both normal and malignant cells. Her laboratory developed novel 3D assays and techniques that demonstrate her signature phrase: after conception, “phenotype is dominant over genotype.”

Bissell earned her doctorate in microbiology and molecular genetics from Harvard Medical School, won an American Cancer Society fellowship for her postdoctoral studies, and soon after joined LBNL. She was the founding Director of the Cell and Molecular Biology Division and later the Associate Laboratory Director for all Life Sciences at Berkeley Lab where she recruited outstanding scientists and developed a strong program in cell and molecular biology and breast cancer.

Bissell has published more than 400 publications and is one of the most sought-after speakers in the field. She has received numerous honors and awards, which include: U.S. Department of Energy’s E.O. Lawrence Award, AACR’s G.H.A. Clowes Memorial Award, the Pezcoller Foundation-AACR International Award, Susan G. Komen Foundation’s Brinker Award, BCRF Foundation’s Jill Rose Award, Berkeley Lab’s inaugural Lifetime Achievement Prize, American Cancer Society’s Medal of Honor, MD Anderson Cancer Center’s highest honor – the Ernst W. Bertner Award, the Honorary Medal from the Signaling Societies in Germany, ASCB’s highest honor – the E.B. Wilson Medal, and the 2017 AACR Award for Lifetime Achievement in Cancer Research. Bissell is an inspiring mentor and in her honor, the University of Porto, Portugal established the Mina J. Bissell Award which is given every three years to a person who has dramatically changed a field. She is the recipient of Honorary Doctorates from both Pierre & Marie Curie University in Paris, France and University of Copenhagen in Denmark. Bissell is not only an elected Fellow of most U.S. honorary scientific academies, including National Academy of Sciences (NAS), National Academy of Medicine (NAM), and American Philosophical Society (APS), but she also sits on many national and international scientific boards and continues to engage in full-time research, among other scientific activities.

Dr. Bissell is presenting in Plenary Session 4 – Tissue Environment and Architecture, on Sunday, 6 May 2018, at 10:30.

11 Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 11 Plenary Speakers

Ana Maria Cuervo, MD, PhD Albert Einstein College of Medicine New York, NY United States

Ana Maria Cuervo is the R.R. Belfer Chair for Neurodegenerative Diseases, Professor in the Departments of Developmental and Molecular Biology and of Medicine of the Albert Einstein College of Medicine and co- director of the Einstein Institute for Aging Studies. She obtained her M.D. and a Ph.D. in Biochemistry and Molecular biology from the University of Valencia (Spain) and received postdoctoral training at Tufts University, Boston. In 2002, she started her laboratory at the Albert Einstein College of Medicine, where she continues her studies in the role of protein-degradation in neurodegenerative diseases and aging. Dr. Cuervo has received prestigious awards such as the P. Benson and the Keith Porter in Cell Biology, the Nathan Shock Memorial Lecture, the Vincent Cristofalo and the Bennett J. Cohen in basic aging biology and the Marshall Horwitz and the Saul Korey Prize for excellence in research and in Translational Medicine. She delivered prominent lectures such as the Robert R. Konh, the NIH Director’s, the Roy Walford, the Feodor Lynen, the Margaret Pittman, the IUBMB Award, the David H. Murdoxk, the Gerry Aurbach and the Harvey Society Lecture. She is currently co-Editor-in-Chief of Aging Cell and has been member of the NIA Scientific Council and of the NIH Council of Councils. Dr. Cuervo is presenting in Plenary Session 1 – Autophagy, Metabolism and Aging, on Thursday, 3 May 2018, at 10:00.

Daniel J. Klionsky, PhD University of Michigan Ann Arbor, MI United States Cell biologist Daniel J. Klionsky, PhD, is the Alexander G. Ruthven Professor of Life Sciences at the University of Michigan. Dr. Klionsky holds joint appointments as a faculty member at the Life Sciences Institute, where his lab is located, and in the Department of Molecular, Cellular and Developmental Biology in the College of Literature, Science, and the Arts. Working primarily with baker’s yeast (Saccharomyces cerevisiae), Dr. Klionsky’s research focuses on the cellular process known as autophagy, which literally means “self-eating.” Autophagy is the process by which cells break down cellular components to survive stress conditions such as starvation. The failure of autophagy plays a role in cancer, neurodegenerative conditions such as Parkinson and Alzheimer diseases, and other areas of human health. Using biochemistry, Dr. Klionsky’s lab has been asking questions about the individual molecules involved in the transport of proteins and cellular signaling related to autophagy. Answering these questions could help guide new therapeutic applications to treat or prevent certain diseases. A native Californian, Dr. Klionsky received an A.B. in Biology from the University of California, Los Angeles in 1980. He received his Ph.D. from Stanford University in 1986, which was followed by Helen Hay Whitney and American Cancer Society Senior Postdoctoral Fellowships at the California Institute of Technology. In 1990, Dr. Klionsky joined the faculty of the University of California, Davis; he was a Fellow of the John Simon Guggenheim Memorial Foundation in 1997. He moved to U-M as a full professor in 2000, and in 2003, because one of the founding faculty members of the U-M Life Sciences Institute. Dr. Klionsky’s passion extends to the classroom as well as the lab. He received the National Science Foundation Director’s Award for Distinguished Teaching

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 12 Plenary Speakers

Scholars in 2003. In 2006, the National Academy of Sciences named him an Education Mentor for developing “active learning” approaches to undergraduate biology courses. He was elected a Fellow of the American Association for the Advancement of Science in 2009, was named a Thompson Reuters Citation Laureate in 2013, and was awarded the van Deenen Medal from the University of Utrecht in 2015. Since the journal’s founding in 2005, Dr. Klionsky has also served as the editor-in-chief of Autophagy.

Dr. Klionsky is presenting in Plenary Session 1 – Autophagy, Metabolism and Aging, on Thursday, 3 May 2018, at 09:30.

Alex Loukas, PhD James Cook University Cairns, Australia

Alex Loukas is a NHMRC Senior Principal Research Fellow at James Cook University (JCU) in Cairns, QLD. He obtained his BSc and PhD from University of Queensland. He conducted postdoctoral work at University of Edinburgh, held an assistant professorship at George Washington University, and led a group at Queensland Institute of Medical Research before moving to JCU Cairns as a professorial tropical research leader in 2010. Loukas’ research interests focus on the molecular basis of host-parasite interactions, and exploiting that knowledge to develop (1) anti-helminth subunit vaccines, and (2) next-generation biologics for treating inflammatory and metabolic diseases using human parasite challenge models and recombinant parasite proteins. His work is currently funded by NHMRC, NIH (US) and various pharmaceutical companies.

Dr. Loukas is presenting in the Plenary Session 3 – EVs in Envrionment, Diet, and Health, on Saturday, 5 May 2018, at 10:30.

Graça Raposo, PhD Institut Curie Paris, France

Graça Raposo received her PhD in 1989 in Membrane Biology and Immunology at the University of Paris VII where she specialized in electron microscopy and membrane biology. From 1990 to 1995 she was a postdoctoral fellow at the Immunology Center in Marseille and then in the Dept of Cell Biology, Utrecht University, The Netherlands. As a CNRS Research Director she is the deputy Director of the Dept of Cell Biology and Director of the Training unit at Institut Curie. Her major research interests focus on the biogenesis and functions of exosomes and lysosome related organelles with implications in neurodegenerative disorders, lysosomal diseases and cancer. She have pioneered studies on exosomes secreted by immune cells but also other cells. Her group also started to unravel the cellular and molecular mechanisms regulating the biogenesis of melanosomes, the lysosome related organelles of epidermal melanocytes, studies that open a new avenue to modulate pigmentation in health and disease. In 2012 she was awarded the CNRS Silver Medal and in 2013 the Descartes Huygens Price from the Royal Dutch Academy of Sciences. Since 2015, she is a member of the European Molecular Biology Organization (EMBO).

Dr. Raposo is presenting in Plenary Session 2 – Advances in Exosomes Biology, on Friday, 4 May 2018, at 11:00.

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 13 Plenary Speakers

Randy Schekman, PhD University of California – Berkeley Berkeley, CA United States

Dr. Randy Schekman is a Professor in the Department of Molecular and Cell Biology, University of California, Berkeley, and an Investigator of the Howard Hughes Medical Institute. He studied the enzymology of DNA replication as a graduate student with Arthur Kornberg at Stanford University. His current interest in cellular membranes developed during a postdoctoral period with S. J. Singer at the University of California, San Diego. At Berkeley, he developed a genetic and biochemical approach to the study of eukaryotic membrane traffic. Among his awards are the Gairdner International Award, the Albert Lasker Award in Basic Medical Research and the Nobel Prize in Physiology or Medicine, which he shared with James Rothman and Thomas Südhof. He is a member of the National Academy of Sciences, the Institute of Medicine, the American Academy of Arts and Sciences, the American Philosophical Society, a Foreign Associate of the Accademia Nazionale dei Lincei, a Foreign Associate of the Royal Society of London and an Honorary Academician of the Academia Sinica. In 1999, he was elected President of the American Society for Cell Biology. In 2002 he was appointed Editor-in-Chief of the Annual Reviews of Cell and Developmental Biology. From 2006 - 2011 he served as Editor-in-Chief of the Proceedings of the NAS. In 2011, he was appointed Editor-in-Chief of an Open Access journal, eLife, sponsored by the HHMI, Wellcome Trust and the Max Planck Society.

Schekman’s laboratory investigates the mechanism of membrane protein traffic in the secretory pathway in eukaryotic cells. His approach began with a genetic and biochemical dissection of the secretory pathway in the yeast, S.cerevisiae. His lab discovered the genes and proteins that assemble proteins into the endoplasmic reticulum membrane, package proteins into coated (COPII) transport vesicles and deliver vesicles by fusion at a target membrane. The genes and proteins his lab discovered in yeast have counterparts in all eukaryotes and have been implicated in several human genetic diseases. The evolutionary conservation of the pathway discovered in Schekman’s lab encouraged the biotechnology industry to use yeast as a platform for the production of clinically important human secreted proteins. Approximately one-third of the world supply of recombinant human insulin is made by secretion in yeast and the entire world supply of recombinant hepatitis b vaccine is made in vesicles produced in yeast. As hepatitis b infection is the major cause of liver cancer in the world, this vaccine promises to reduce the incidence of liver cancer by 90%. In recent years his lab has turned from yeast to mammalian cell culture to investigate aspects of human physiology and disease that are not readily studied in yeast.

Dr. Schekman is presenting Plenary Session 2 – Advances in Exosomes Biology, on Friday, 4 May 2018, at 10:30.

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 14 Program

WEDNESDAY, 2 MAY 2018 Education Day

07:30 – 20:00 Registration/Information Desk Level 1 Foyer

The Pre-meeting Education Day is a successful tradition of the annual ISEV meetings. Focusing on particular topics within the extracellular vesicle field, select experts provide deep insight into certain basic aspects of extracellular vesicles. These educational presentations collectively illuminate burning questions and provide a comprehensive insight into the topic.

08:30 – 18:00 PARALLEL SESSIONS Room Room 6 Room 5 TRACK A: TRACK B: EVs in Biology and Technology EVs in Health and Disease 08:30 Welcome Welcome – Juan M. Falcón-Pérez, CIC bioGUNE, Spain – Hernando del Portillo, ICREA at ISGlobal and IGTP, Spain 08:35 Overview of Extracellular Vesicles (EV) Introduction to EVs in Biomarkers and Therapeutics – Edit Buzas, Semmelweis University, Hungary – Clotilde Thery, Curie Institute, France 09:00 - 10:30 SESSION 1: Biology of EVs SESSION 1: Introduction to EV as Biomarkers and Their Role in Cancer 09:00 Overview on EV Nomenclature, Biogenesis and EV Isolation and Storage for Biomarker Cargo Selection Discovery: Biobanking – María Yáñez-Mó, Universidad Autónoma de – Takahiro Ochiya, National Cancer Center, Japan Madrid, Spain 09:20 Overview on EV Uptake: Keys in the Interaction of EVs EV-based Biomarkers in Cancer with Recipient Cells – Guido Jenster, Erasmus Medical Center, – Pieter Vader, University Medical Center, The Netherlands The Netherlands 09:40 Overview on Current Methodologies Widely Used in Overview of EVs in Cancer Establishment, Progression, EV Research, Pros, Cons, and Pitfalls and Metastasis – Jan van Deun, Ghent University, Belgium – Carolina Soekmadji, QIMR Bergofer, Medical Research Institute, Australia 10:00 Chaired Q and A Discussion Session with all Speakers Chaired Q and A Discussion Session with all Speakers – María Yáñez-Mó, Universidad Autónoma de – Takahiro Ochiya, National Cancer Center, Japan Madrid, Spain 10:30 - 10:50 Coffee Break Coffee Break 10:50 - 12:20 SESSION 2: Isolation and Quantification: SESSION 2: EVs in the Immune System Methods and Challenges and Inflammation 10:50 Quality Controls of EV Preparations and Methods to EVs in Immune System Regulation Improve Their Purity – Francesc Borras, Germans Trias i Pujol Research – Aled Clayton, Cardiff University, United Kingdom Institute (IGTP), Spain 11:10 What's new in EV Isolation? Acoustics/Microfluidics EVs in Tumor Immune Response – Victor Ugaz, Texas A&M University, United States – Susanne Gabrielsson, Karolinska Institute, Sweden

To accommodate author/presenter requests and/or cancellations, the presentation numbering may be out of sequence. Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 15 Program WEDNESDAY, 2 MAY 2018

Room Room 6 Room 5 TRACK A: TRACK B: EVs in Biology and Technology EVs in Health and Disease 11:30 On-a-chip Technology for Light-based EV Detection EVs in Regenerative Neuroimmunology – Romain Quidant, ICFO, Spain – Stefano Pluchino, University of Cambridge, United Kingdom 11:50 Chaired Q and A Discussion Session with all Speakers Chaired Q and A Discussion Session with all Speakers – Aled Clayton, Cardiff University, United Kingdom – Francesc Borras, Germans Trias i Pujol Research Institute (IGTP), Spain 12:20 - 13:30 Lunch Break Palau 4 Lunch Break Palau 4 13:30 - 15:20 SESSION 3: Characterisation and Phenotyping SESSION 3: Pathogen-derived EVs of EVs 13:30 High Resolution Flow Cytometry EVs in Malaria – Jennifer Jones, NIH, United States – Hernando del Portillo, ICREA at ISGlobal and IGTP, Spain 13:50 Pitfalls in EV Electron Microscopy and EV Labelling EVs in Parasitic Helminths – Graça Raposo, Curie Institute, France – Antonio Marcilla, Universitat de València, Spain 14:10 Unravelling Exosome Biogenesis Using Super- EVs in Bacteria resolution 3D-SIM Microscopy in Fly and Human – Elena Mercadé, University of Barcelona, Spain Cell Models – Deborah Goberdhan, Oxford University, United Kingdom 14:30 Genetic Labeling Strategies to Track Endogenous, The Multifaceted Relationship between EV Un-manipulated EV and their Target Cell Types in vivo and Viruses – Ferdinando Pucci, Massachusetts General Hospital, – Esther Nolte-`t Hoen, Utrecht University, The Netherlands United States 14:50 Chaired Q and A Discussion Session with all Speakers Chaired Q and A Discussion Session with all Speakers – Jennifer Jones, NIH, United States – Hernando del Portillo, ISGlobal, Spain 15:20 Coffee Break Coffee Break 15:50 - 17:40 SESSION 4: Advance in Profiling EV Content SESSION 4: EVs in Therapeutics Applications 15:50 Dos and Don’ts in EV Proteomics and EV Surface Strategies for EV Targeting and Modification Molecule Analysis – Raymond M. Schiffelers, University Medical – Suresh Mathivanan, LaTrobe University, Australia Center Utrecht, The Netherlands 16:10 Making Sense of exRNA: The Big and The Small Bacterial Membrane Vesicles as Therapeutics – Amy Buck, University of Edinburgh, Scotland – Hanne Winther Larsen, University of Oslo, Norway 16:30 Computational Deconvolution of EV Cargo EVs as Vaccines – Aleksandar Milosavljevic, Baylor College of Medicine, – Yong Song Gho, Postech, Korea United States 16:50 Cytokines in EV and Their Analysis Regulatory and Manufacturing Aspects for Clinical – Leonid Margolis, NIH, United States Applications of EVs – Karin Pachler, SCI-TReCS, PMU, Austria 17:10 Chaired Q and A Discussion Session with all Speakers Chaired Q and A Discussion Session with all Speakers – Suresh Mathivanan, LaTrobe University, Australia – Raymond M. Schiffelers, University Medical Center Utrecht, The Netherlands Wrap up of Track A Wrap up of Track B 17:40 - 18:00 – Dave Carter, Oxford Brookes University, United Kingdom – Bernd Giebel, University Hospital Essen, Germany

Satellite Symposia Room 3 See page 35 for agendas for both Symposia

18:00 – 20:30 Emerging Trends in EVs associated with HIV & Drug Abuse Room 4

18:00 – 19:30 Research Methods for Extracellular RNA Room 3

THURSDAY, 3 MAY 2018 ISEV2018 Annual Meeting

07:30 – 20:00 Registration/Information Desk Level 1 Foyer

08:00 – 09:00 Networking Coffee Break and Exhibits Level 1 Foyer

09:00 – 09:30 Welcome Remarks/Opening Ceremony Auditorium Special performance by tenor Sergi Gimenez Carreras

09:30 – 10:30 PLENARY SESSION 1: Autophagy, Metabolism, and Aging Auditorium Session Chairs: Juan Falcón-Pérez; Andrew Hill

09:30 Introduction and Lecture: The Mechanism and Regulation of Authophagy – Daniel J. Klionsky, PhD 10:00 Introduction and Lecture: A Way Out When Selective Autophagy Fails in Aging – Ana Maria Cuervo, MD, PhD

10:30 – 10:45 Networking Coffee Break and Exhibits Level 1 Foyer

10:45 – 12:15 PARALLEL SESSIONS Room Auditorium Room 5 Room 6 SYMPOSIUM SESSION 1: SYMPOSIUM SESSION 2: SYMPOSIUM SESSION 3: EVs in Metabolic Disorders EV and The Immune System EVs as Therapeutic Agents Session Chairs: Session Chairs: Session Chairs: Juan Falcón-Pérez; Susmita Sahoo Francesc Borrás; Ester Nolte-‘t Hoen Yong Song Gho; Ewa Zuba Surma 10:45 OT01.01 – The bystander effect OT02.01 Exosomal transfer of OT03.01 Extracellular vesicles of aging microRNAs during immune synapsis released by mesenchymal stem – Ana O'Loghlen contributes to the fine-tuning of cells represent a novel therapeutic immune responses option in systemic sclerosis – Lola Fernandez Messina – Daniele Noel 11:00 OT01.02 – Protective effects of OT02.02 – DNA outside and inside of OT03.02 – Neoadjuvant caloric restriction on Alzheimer's EVs and its role in phosphorylation chemotherapy elicits a pro- Disease progression: Role for of Interferon Regulatory Factor 3 metastatic cascade through EVs choroid plexus derived – Elisa Lázaro-Ibáñez and monocytes extracellular vesicles? – Ioanna Keklikoglou – Charysse Vandendriessche

Room Auditorium Room 5 Room 6 11:15 OT01.03 – Diabetes mellitus drives OT02.03 – Cigarette smoke-exposed OT03.03 Shedding a bevacizumab extracellular vesicle secretion and monocyte-derived dentritic cells in tumour cells-derived extracellular promotes increased internalization release EVs that elicit TH1/TH17 vesicles as a new therapeutic by circulating leukocytes responses in autologous T-cells resistance mechanism in – Nicole Noren Hooten – Kenneth Witwer glioblastoma – Thomas Simon 11:30 OT01.04 – Extracellular vesicles in OT02.04 – Mesenchymal stromal OT03.04 – Synergistic effect of Type 1 diabetes: Comparison of cell extracellular vesicles modulate extracellular vesicles loaded with immunological properties of divers innate and adaptive immune cells oncolytic viruses and paclitaxel for subpopulations of extracellular at multi-organ level in a model of cancer drug delivery vesicles of the pancreatic beta bronchopulmonary dysplasia – Mariangela Garofalo cell secretome – Monica Reis – Steffi Bosch 11:45 OT01.05 – Higher levels of OT02.05 – Annexin a5(An5)-bound OT03.05 – Milk exosomes - extracellular vesicles in Type 1 extracellular vesicles (EVs) from A 'platform' nano-carrier siRNA diabetes: A cohort study of 236 mesenchymal stromal cells (MSCs) delivery patients show enhanced and specific anti- – Ramesh C. Gupta – Sara Tehrani inflammatory effects – Maurizio Muraca 12:00 OT01.06 – Characterization of OT02.06 – Human mesenchymal OT03.06 – Bovine milk-derived the exosomal proteins and their stem cells or extracellular extracellular vesicles can inhibit potential as regulators of systemic vesicles derived from these cells catabolic and inflammatory metabolism administered intra-arterially mediators in articular chondrocytes – Ruben Garcia Martin modulate immune response in a and fibroblast-like synoviocytes rat model of ischemic brain injury from osteoarthritis patients – Sylwia Dabrowska – Bartijn Pieters

12:15 – 13:30 Lunch Palau 4

12:15 – 13:30 Posters/Exhibits Level 1 Foyer

13:00 – 13:20 Projection of 2D-3D Animation: Extracellular Vesicles - The Cell’s Secret Messengers Auditorium Session Chair: Dave Carter

13:30 – 15:00 PARALLEL SESSIONS Room Auditorium Room 5 Room 6 SYMPOSIUM SESSION 4: SYMPOSIUM SESSION 5: SYMPOSIUM SESSION 6: EVs as Biomarkers EVs in Stem Cell and Cancer Growth EV Analysis by Microfluidics and Session Chairs: Session Chairs: Flow Cytometry Carla Oliveira; Takahiro Ochiya Lorraine O’Driscoll; Peter Quesenberry Session Chairs: Jennifer Jones; Victor Ugaz 13:30 OT04.01 – EV analysis of clinical OT05.01 – Oncogenic EGFR/ OT06.01 – Microfluidic device urine samples from prostate Src signaling modulates the provides a solution for a time- cancer patients functionality of exosomal β4 course analysis of EV secretion – Martin E. van Royen integrin for tumor malignancy and – Takanori Ichiki organotropic metastasis – Tang-Long Shen 13:45 OT04.02 – A novel strategy to OT05.06 – Increased hematopoietic OT06.02 – Rapid quantification liquid biopsy for early diagnosis extracellular RNAs and vesicles and characterization of individual of lethal prostate cancer in the lung during allergic airway urinary EVs using a microfluidic employing palmitoyl-proteomics of responses assay extracellular vesicles – Heather Pua – Serhii Mytnyk – Javier Mariscal 14:00 OT04.03 – Development of a OT05.03 – Oral administration of OT06.03 – Exosome nanoarray for multiplex-to-single exosome bovine milk-derived extracellular the next generation single-exosome analysis (MT-SEA) pipeline to vesicles reduce primary tumor analysis platform characterize exosomes associated burden but accelerate cancer – Kyohei Okubo with tumor progression and metastases responses to treatment – Suresh Mathivanan – Joshua Welsh 14:15 OT04.04 – Plasma extracellular OT05.04 – 3D culture of cancer cells OT06.04 – Hollow organosilica vesicle imaging by high resolution in a polysaccharide-based hydrogel beads as reference particles for flow cytometry in patients drastically alters the protein and optical detection of extracellular presenting for diagnostic EUS- RNA cargo of EVs vesicles guided pancreatic FNA – Christopher Millan – Zoltan Varga – Terry K. Morgan 14:30 OT04.05 – Exosomes and OT05.05 – lncRNA 19 from human OT06.05 – Bioengineered microvesicles contain more tumor adipose stem cells derived nanovesicles as extracellular RNA than platelets exosomes promote the vesicle-mimetics: A novel standard – Kay Brinkmann regeneration of hepatocytes by for extracellular vesicle analysis up-regulating HGF/c-Met pathway – Estefania Lozano-Andres and significantly improve survival of rats with acute liver failure – Yinpeng Jin 14:45 OT04.06 – Clinical relevance of the OT05.02 – The role of large OT06.06 – Isolation of microvesicles defining the most abundant fraction oncosomes in leukaemia and exosomes by fluorescence- of mutated oncogenic DNA and RNA – Valentina R. Minciacchi triggered FACS among different EV subtypes – Alain Brisson – Irina Nazarenko

15:00 – 15:30 Networking Coffee Break and Exhibits Level 1 Foyer

15:30 – 16:30 PARALLEL SESSIONS Room Auditorium Room 5 Room 6 ORAL WITH POSTER SESSION 1 ORAL WITH POSTER SESSION 2 ORAL WITH POSTER SESSION 3 EV Functional Studies in EV Biomarkers and Biogenesis, Signaling Different Models Subpopulations & Characterization Session Chair: Session Chair: Session Chair: Hernando del Portillo Francesc Borrás Maria Yañez-Mó 15:30 OWP 1.01 – Immunomodulatory OWP 2.01 – Isolation and phenotype OWP 3.01 – Sarco/Endoplasmic function of human mesenchymal characterization of microvesicle reticulum ATPase inhibition stromal cells-derived extracellular subpopulations from mixed activates calcium signaling pathways vesicles on Type-I interferon cells in an vitro model of lung for microvesicle biogenesis response in human plasmacytoid microvascular injury – Jack D. Taylor dendritic cells and lupus murine – Nikhil Tirlapur pDCs – Lin Kui 15:35 OWP 1.02 – Role of CD44 in OWP 2.02 – Detection and OWP 3.02 – Origin of extracellular therapeutic mesenchymal stem characterization of different vesicles released during exhaustive cell-derived extracellular vesicles neuronal and glial populations exercise for joint diseases of exosomes by surface plasmon – Alexandra Brahmer – Enrico Ragni resonance imaging – Alice Gualerzi 15:40 OWP 1.03 – Osteoblast-secreted OWP 2.03 – Microscale OWP 3.03 – Extracellular vesicles extracellular vesicles stimulate electrophoretic separations of as mediators of periphery-to-brain the expansion of CD34+ human exosomes communication in inflammation umbilical cord blood cells – Yuliya Shakalisava associated brain disorders – Jess Morhayim – Nagiua Haymour 15:45 OWP 1.04 – Prostate cancer-derived OWP 2.04 – Normalization of OWP 3.04 – Extracellular vesicles extracellular vesicles facilitate urinary extracellular vesicles deformation on surface: Some osteoclast fusion and differentiation – Charles J. Blijdorp tracks to limit it via enhancing filopodia formation – Ksenia Maximova in osteoclast precursors – Fumihiko Urabe 15:50 OWP 1.05 – Investigating the roles OWP 2.05 – Comparative analysis OWP 3.05 – Comparison of generic of macrophage colony-stimulating of raman signals between non-small fluorescent dyes for detection factor (CSF-1) and carbonic cell lung cancer (NSCLC) cell-derived of extracellular vesicles by flow anhydrase 9 (CAIX) in neratinib exosomes and their potential cytometry resistant HER2+ breast cancer cell protein markers – Leonie de Rond lines and extracellular vesicles – Hyunku Shin – Michelle C. Lowry 15:55 OWP 1.06 – Extracellular vesicles OWP 2.06 – Development of High OWP 3.06 – Role of calcium isolated from cardiosphere-derived Sensitivity Flow Cytometry for Sizing signaling in the biogenesis of cells and mesenchymal stem cells and Molecular Profiling of Individual different types of extracellular elicit distinct immunomodulatory Extracellular Vesicles Down to 40 nm vesicles derived from the same cell properties in vitro and in vivo – Xiaomei Yan – Ákos Lőrincz – Geoffrey de Couto 16:00 OWP 1.07 – Role of Wnt4 exosomes OWP 2.07 – Immunofluorescence OWP 3.07 – Unraveling the in thymic aging flow cytometry of extracellular distribution of extracellular – Krisztina Banfai vesicle surface proteins vesicles in vivo using recombinant – John Nolan tetraspanins – Stefan Vogt

To accommodate author/presenter requests and/or cancellations, the presentation numbering may be out of sequence. Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 20 Program THURSDAY, 3 MAY 2018

16:05 OWP 1.08 – Impact of pathogenic OWP 2.08 – Free flow OWP 3.08 – Evidence for selective microbes and healthy microbiota by electrophoresis allows preparation mRNA sorting into cancer exosomes Lactobacillus-derived extracellular of extracellular vesicles fractions – Mohammad Arshad.Aziz vesicles with high recovery and purity rates –Bao-Hong Lee – Christian Reiter 16:10 OWP 1.09 – Catching the hedgehog: OWP 2.09 – Isolation of extracellular OWP 3.09 – Alterations in the Unraveling hedgehog secretion vesicle-associated small RNA from miRNA cargo of HIV-infected during filopodia-mediated transport canine mitral valve interstitial macrophage-derived extracellular – Gustavo Aguilar cells using ultracentrifugation and vesicles promote pulmonary tangential flow filtration with size smooth muscle proliferation exclusion chromatography – Navneet K. Dhillon – Vicky Yang

16:30 – 17:15 The EV Blood Roadmap Auditorium 16:30 Welcome - Chris Gardiner 16:35 Introduction - Rienk Nieuwland 16:45 Biospecimen - Aled Clayton 16:50 Pre-analytical Variables - Kenneth Witwer 17:00 EV TRACK - An Hendrix 17:05 Plenary Discussion - All speakers

16:30 – 17:15 Meet the Journal Editors Room 5 Chair: Hector Peinado; Marca Wauben 16:35 Nature Communications – Simona Fiorani, Team Manager and Senior Editor 17:45 PLOS|Biology – Ines Alvarez-Garcia, Senior Editor 16:55 Science Matters – Lawrence Rajendran, Founder and CEO 17:05 Journal of Extracellular Vesicles – Peter J. Quesenberry, Co-editor in Chief

17:15 – 18:30 Thursday Poster Session 1 (PT) Level 1 Foyer PT01: EVs, Pathogens and Cross-organism Communication PT07: EV-inspired Therapeutics, Vaccines, and Clinical Trials Parasitic Infections Session Chairs: Shilpa Buch; Pia Siljander Session Chairs: Martin Jaular Lorena; Maria Elena Mercade PT08: Cardiovascular Insult PT02: EVs in Reproduction and Pregnancy Session Chairs: J. Brian Byrd; Navneet Dogra Session Chairs: Eva Colas; Maria Yañez-Mó PT09: EVs in Autoimmunity and Sepsis PT03: EV-OMICS Session Chairs: Lola Fernandez Messina; Fabiana Geraci Session Chairs: Armando de Menezen; Muller Fabbri LBT01: Methodology PT04: Tumor-stroma Interactions by EVs Session Chairs: Muthuvel Jayachandran; Theresa Whiteside Session Chairs: Carla Mazzeo; Michiel Pegtel LBT02: Cancer I PT05: EVs as Cancer Biomarkers-proteomics Session Chairs: Chiara Ciardiello; Pilar Sepulveda Session Chairs: Yves deClerck; Alicia Llorente LBT03: OMICS PT06: EVs in Cellular Differentation and Organ Development Session Chairs: Emma Guns; Elisa Lázaro-Ibáñez Session Chairs: Guilaume van Niel; Ana Gámez-Valero

18:30 – 20:00 Meet the Expert Session Auditorium RNA and EVs: The What, Why and Where of their Interaction? Session Chair: Andrew Hill 18:30 RNA in liquid biopsies – Louise Laurent, University of California - Davis, USA 18:50 Exploring the small RNA world of extracellular vesicles – Esther Nolte-‘t Hoen, Utrecht University, The Netherlands 19:10 Functional aspects of small RNAs in EVs related with aging – Joannes Grillari, Medical University of Vienna, Austria

18:30 – 20:00 Meet the Expert Session Room 5 Regulatory Aspects of EVs to Reach the Clinic Session Chair: Susmita Sahoo 18:30 Manufacturing license vs. market authorization - Crossroads for therapeutic EV development – Mario Gimona, Paracelsus Medical University, Austria 18:50 Exosomes vaccines in veterinary: A challenge – Lorenzo Fraile, University of Lleida, Spain 19:10 Regulatory aspects of EVs to reach the clinic – Xavier Luria, Drug Development and Regulation, Spain 19:30 Extracellular vesicles as medicinal product: An evolving regulatory perspective – Rosaria Giordano, Fondazione Ospedale Maggiore Policlinico, Italy

18:30 – 20:00 Meet the National Societies and Outreach Strategies Room 6 Session Chair: Isabel Guerrero This event will provide an opportunity for scientific networks and consortia focusing on extracellular vesicles to introduce themselves to the ISEV community. See agenda for this session on page 38.

To accommodate author/presenter requests and/or cancellations, the presentation numbering may be out of sequence. Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 22 Program

FRIDAY, 4 MAY 2018

07:30 – 20:00 Registration/Information Desk Level 1 Foyer

08:30 – 10:00 PARALLEL SESSIONS Room Auditorium Room 5 Room 6 SYMPOSIUM SESSION 10: SYMPOSIUM SESSION 11: SYMPOSIUM SESSION 12: EV Biogenesis and Update EVs and Metastatic Niches EV Characterization: Session Chairs: Session Chairs: State-of-the-Art Approaches Isabel Guerrero; Guillaume van Niel Vincenza Dolo; Jacky Goetz Session Chairs: Irina Nazarenko; Rienk Nieuwland 08:30 OF10.01 – Following the trafficking OF11.01 – Breast cancer-derived OF12.01 – Gold nanoparticle ring of extracellular vesicles markers extracellular vesicles modulate the and hole structures-based platforms to understand the biogenesis of activity of signaling pathways in the for capture and label-free detection different extracellular vesicles brain microenvironment of exosomes subtypes – Golnaz Morad – Duraichelvan Raju – Mathilde Mathieu 08:45 OF10.02 – A novel conserved OF11.02 – Zebrabow as in vivo model OF12.02 – Vibrational spectroscopy exosome biogenesis pathway system to monitor vesicles mediated as a tool for fingerprinting tumor mediates adaptive response to transfer in cancer exosomes microenvironmental stress in – Martin E. van Royen – Randy Carney cancer cells – Deborah Goberdhan 09:00 OF10.03 – HA-EVs are a unique OF11.03 – Pre-metastatic cancer OF12.03 – "None of us is the same species of EV with diverse properties exosomes induce immune as all of us”: Nanoscale probing of and widespread biological relevance surveillance by patrolling monocytes heterogeneity of stem-cell derived – Kirsi Rilla – Olga Volpert extracellular vesicles by resonance enhanced atomic force microscope infrared spectroscopy – Wojciech Chrzanowski 09:15 OF10.04 – The integrin Mac1/CR3 OF11.04 – Melanoma-derived OF12.04 – Membrane protein plays a central role in production exosomes reinforce metastasis by quantification on extracellular and cargo editing of EVs issued from inducing lymphangiogenesis and vesicles by surface plasmon nuetrophilic granulocytes impairing dendritic cell function resonance imaging and time- – Erzsébet Ligeti – Hector Peinado resolved fluorescence immunoassay – Frank A.W. Coumans 09:30 OF10.05 – Extracellular vesicle OF11.05 – Paracrine mechanisms OF12.05 – Proximity assays for budding is inhibited by redundant induced by large oncosomes detection and characterization regulators of TAT-5 flippase spontaneously shed by aggressive of exosomes localization and phospholipid cells to promote adhesion and – Masood Kamali-Moghaddam asymmetry invasion of prostate cancer via an – Ann M. Wehman integrin-dependent activation of FAK-AKT pathway – Chiara Ciardiello 09:45 OF10.06 – Uptake of extracellular OF11.06 – Circulating large OF12.06 – Individual EV visualization vesicles in a cell free extract EVs in plasma of patients with using TIRF microscopy for EV – Gregory Lavieu metastatic prostate cancer contain subpopulation study chromosomal DNA and report – Chungmin Han cancer-specific genomic alterations – Dolores Di Vizio

10:00 – 10:30 Networking Coffee Break and Exhibits Level 1 Foyer

10:30 – 11:45 PLENARY SESSION 2: Advances in Exosome Biology Auditorium Session Chairs: Antonio Marcilla; Marca Wauben 10:30 Introduction and Lecture: Mechanism of small RNA sorting into exosomes secreted by cultured human cells – Randy Schekman, PhD 11:00 Introduction and Lecture: Advances on the cell biology of extracellular vesicles – Graça Raposo, PhD

11:45 – 12:20 Featured Abstract Session 1 Auditorium Session Chair: Clotilde Thery 11:45 Introduction 11:50 FA.01 – Effect of tetraspanin-blocking peptides in the biogenesis of exosomes in melanoma cells – Carla Mazzeo 11:50 FA.02 – Mechanisms for the exosomal secretion and transmission of α-synuclein in the brain – Jason Howitt

12:20 – 13:45 Lunch Palau 4

12:20 – 13:45 Posters/Exhibits Level 1 Foyer

12:40 – 13:45 Young Career Development on Academy and Industry in the Field of Extracellular Vesicles Room 3 See agenda for this session on Page 36.

12:40 – 13:45 ISEV ISAC ISTH EV Flow Cytometry Work Group Room 4 This satellite event will provide insight into the scientific interaction of three big international societies, and will also give opportunity for young researchers to present the first results of this collaboration.

13:45 – 15:15 PARALLEL SESSIONS Room Auditorium Room 5 Room 6 SYMPOSIUM SESSION 13: SYMPOSIUM SESSION 14: SYMPOSIUM SESSION 15: Role of EVs in Cell-Cell Tissue Injury and Repair EVs and the Nervous System Communication Session Chairs: Session Chairs: Session Chairs: Bernd Giebel; Mariko Ikuo Andrew Hill; David Otaegui Antonella Bongiovanni; Hector Peinado 13:45 OF13.01 – Computer guided image OF14.01 – Human neural stem OF15.01 – Study of exosomal analysis of nuclear membrane cell extracellular vesicles improve microRNAs from microglia involved instability in tissues reveals clinical recovery in a porcine model of in neuroprotection in Hirudo relevance for nucleus-derived EVs ischemic stroke Medicinalis – Andries Zijlstra – Robin Webb – Quentin Lemaire 14:00 OF13.02 – 3D culture modelling OF14.02 – Hypoxia modifies the OF15.02 – Extracellular vesicle illustrates a role for EVs in mediating release of extracellular vesicles by associated microRNA-29a elicits the biomechanics of the tumour mesenchymal cells improving renal microglial inflammation and microenvironment recovery after ischemia-reperfusion synaptodendritic injury during – Nicholas Peake injury chronic methamphetamine abuse – Rafael S. Lindoso – Sowmya V. Yelamanchili 14:15 OF13.03 –Melanoma-derived OF14.03 – Human induced OF15.03 – Apolipoprotein E4 microvesicles are taken up by pluripotent stem cell extracellular compromises brain exosome lymph node resident macrophages vesicles trigger a MiRNA-dependent production and secretion and lymphatic endothelial cells and anti-inflammatory mechanism to – Katherine Y. Peng induce lymph node remodeling tackle ischemia – Lothar Dieterich – Mario Barilani 14:30 OF13.04 – GD3 ganglioside-enriched OF14.04 – Exosomal miR-29 OF15.04 – Conditional deletion of extracellular vesicles stimulate mediates the therapeutic effects Rab35 and Alix in mice to study melanocyte migration of placenta-derived mesenchymal exosomes in neuron-glia interaction – Andreia H. Otake stromal cells in Duchenne Muscular in vivo Dystrophy – Kerstin Miebach – Chaya Brodie 14:45 OF13.05 – Exosomal heparan OF14.05 – Molecular content and OF15.05 – Interrelationships sulphate proteoglycans (HSPGs) regenerative potential of EVs from between endosomal pathology and drive induction of a pro-angiogenic native and genetically modified exosomal generation and release in stromal cell phenotype induced pluripotent stem cells in neurodegenerative disorders – Alex P. Shephard heart repair in vivo – Efrat Levy – Ewa K. Zuba-Surma 15:00 OF13.06 – CD44 is a novel homing OF14.06 – Opioid-mediated OF15.06 – Immunomodulatory receptor for extracellular vesicles extracellular vesicle production and treatment approach to CNS injury: – Kai Harkonen NLRP3 inflammasome activation Role of mesenchymal stem cell cause vascular damage derived extracellular vesicles – Stephen R. Thom – Amit K. Srivastava

15:15 – 15:45 Networking Coffee Break and Exhibits Level 1 Foyer

15:45 – 16:45 PARALLEL SESSIONS Room Auditorium Room 5 Room 6 SYMPOSIUM SESSION 16: SYMPOSIUM SESSION 17: SYMPOSIUM SESSION 18: Strategies for Studying Alterations in EV Stability EV-inspired Therapeutics in Blood-related EVs and Function Veterinary Medicine Session Chairs: Session Chairs: Session Chairs: Edit Buzas; Chris Gardiner Carmen Fernandez; Marca Wauben; Hanne Winther Larsen Ana Claudia Torrecilhas 15:45 OF16.01 – Rapid isolation of artificial OF17.01 – Overexpression of miR- OF18.01 – Targeted-pig trial on liposomes and exosomes extracted 504 in glioma stem cells inhibits safety and immunogenicity of from plasma of healthy donors the oncogenic potential and serum-derived exosomes obtained utilizing a novel insulator-based the crosstalk of these cells with from porcine respiratory and dielectrophoretic device microglia via exosomal delivery reproductive virus infections – Leyla Esfandiari – Chaya Brodie – Sergio R. Montaner Tarbes 16:00 OF16.02 – Release of OF17.02 – Pathogen-derived OF18.02 – ARMMs as a versatile mitophagosomes from TRAP- extracellular vesicles mediate platform for intracellular delivery of activated platelets division of labour virulence in fatal macromolecules – Silvia H. De Paoli human fungus cryptococcus gattii – Quan Lu – Ewa Bielska 16:15 OF16.03 – What are we looking at? OF17.03 – The role of extracellular OF18.03 – Chitosan coated Extracellular vesicles, lipoproteins, vesicles in mediating radiation- extracellular vesicles as an adjuvant or both? induced bystander effects in the for immunization against salmonid – Jens B. Simonson haematopoietic system rickettsial septicemia in an adult – Katalin Lumniczky zebrafish model – Julia Tandberg 16:30 OF16.04 – Acetylcholinesterase OF17.04 – The RNA-binding protein OF18.04 – Level of extracellular activity co-isolates minimally with hnRNPA2B1 inhibits the export of vesicles, carrying the fibrinolytic small EVs and does not correlate miR-503 into exosomes activator tPA, is reduced in coronary with particle count – Jennifer Perez Boza venous blood during stimulation of – Dillon C. Muth cardiac sympathetic nerves in pigs – Trude Aspelin

16:45 – 17:15 Sponsor Presentations Auditorium Session Chair: Hector Peinado 16:45 Gold Sponsor – Beckman Coulter Life Sciences 17:00 Gold Sponsor – Particle Metrix

17:15 – 18:30 Friday Poster Session 2 (PF) Level 1 Foyer PF01: Analysis of EVs in Liquid Biopsy PF07: EVs in Diseases of the Nervous System (Storage, Preparative Studies, Spike-ins, etc) Session Chair: Eva Maria Kramer-Albers; Tine Hiorth Schøyen Session Chairs: Esperenza Gonzalez; Jaesung Park PF08: EVs in Cardiovascular Diseases and Coagulation PF02: EVs in Cancer: Surrogate Marker Session Chairs: Ramaroson Andriantsitohaina; Session Chairs: Cecilia Lasser; Sonia Melo Costanza Emanueli PF03: EVs and Stem Cells (Including Cancer) PF09: EVs, Pathogens and Cross Organism Communication Session Chairs: Alena Ivanova; Marta Monguió Session Chairs: Anush Arakelyan; Joanne Lannigan PF04: EVs and the Immune System LBF04: Pathogens Session Chairs: Mar Vales; Martin van Herwijnen Session Chairs: Dolores Bernal; Peter Nejsum PF05: EV-based Non-cancer Biomarkers LBF05: RNA Session Chairs: Anabela Cordeiro; Melissa Gualdron Session Chairs: Louise Laurent; Lorraine O’Driscoll PF06: Novel Developments in EV Isolation LBF06: Neurobiology Session Chairs: Carmen Fernandez; Felix Royo Session Chairs: Chaya Brodie; Lesley Cheng

18:30 – 20:00 Meet the Expert Session Auditorium In Vivo Imaging on EVs Session Chair: Hector Peinado 18:30 Tracking tumor extracellular vesicles in vivo at high spatio-temporal resolution – Jacky Goetz, Tumor Biomechanics Laboratory, France 18:50 Current methods to study the in vivo actions of cancer EVs – Michiel Pegtel, VU University Medical Center, The Netherlands 19:10 Exovesicles as carriers of the morphogenetic Hedgehog signal during epithelial development – Isabel Guerrero, Center of Molecular Biology, Spain 19:30 Intravital imaging of extracellular vesicle exchange in cancer – Anoek Zomer, University of Lausanne, Switzerland

18:30 – 20:00 Meet the Expert Session Room 5 EVs on Immunology and Vaccines Session Chair: Mar Vales 18:30 Studying immunity to EVs: In vitro versus in vivo – Susanne Gabrielsson, Karolinska Institute, Sweden 18:50 Plasma-derived exosomes in cancer as potential biomarkers of disease progression and immune competence – Theresa I. Whiteside, University of Pittsburgh, United States 19:10 EV-secreted and non-secreted alpha-Gal glycotopes as vaccine and biomarkers for Chagas disease – Igor C. Almeida, University of El Paso, United States 19:30 Common and specific effects of EV subtypes in immune responses, consequences for vaccine applications – Clotilde Thery, Institut Curie, France

18:30 – 20:00 Meet the Expert Session Room 6 Biobanks for EVs Session Chair: Juan Falcón-Pérez 18:30 The importance of reference controls for EVs characterization: Role of biobanks – Jose Antonio Lopez, Fundacion Instituto Valenciano de Oncologia, Spain 18:50 Applicability of extracellular vesicles for biobank research – Maiha Puhka, Institute for Molecular Medicine, Finland 19:10 Doing a biobank job and getting away with it! – Ryan Pink, Oxford Brookes University, United Kingdom 19:30 Sample collection for successful exosome based diagnostics – Johan Skog, Exosomes Diagnostics, United States

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 27 Program

SATURDAY, 5 MAY 2018

07:30 – 20:00 Registration and Information Desk Level 1 Foyer

08:30 – 10:00 PARALLEL SESSIONS Room Auditorium Room 5 Room 6 SYMPOSIUM SESSION 19: SYMPOSIUM SESSION 20: SYMPOSIUM SESSION 21: Cross-organism Communication EVs As Shuttles of Genetic Material Role of EVs in Regulating Tumor in Bacterial Infections Session Chairs: Cell Behavior Session Chairs: Kwang Pyo Kim; Kenneth Witwer Session Chairs: Igor Almeida; Cherie Blenkiron Susanne Gabrielsson; Jason Webber 08:30 OS19.01 – Transcytosis of OS20.01 – Systematic evaluation OS21.01 – IncRNA HOTAIR affects extracellular vesicles produced of techniques for the isolation and EMT and extracellular vesicle by bacillus subtilis 168 in human detection of small non-coding RNA content and function intestinal Caco-2 Cell monolayers from urine-derived extracellular – Carla J. Beckham – Ana Paula Dominquez Rubio vesicles – Elena S. Martens-Uzunova 08:45 OS19.02 – Explosive cell lysis is OS20.02 – Extracellular vesicles OS21.02 – RASSF1C oncogene required for membrane vesicle mediate the horizontal transfer of an promotes amoeboid phenotype and biogenesis in pseudomonas active LINE-1 retrotransposen invasiveness via extracellular vesicle aeruginosa biofilms – Yumi Kawamura transfer in breast cancer – Cynthia B. Whitchurch – Maria Laura Tognoli 09:00 OS19.03 – Extracellular vesicles OS20.03 – Diverse long-RNAs are OS21.03 – Role of exosomes in secreted by bacteria induce host differentially sorted into exosomes controlling directional migration of cell apoptosis secreted by mutant KRAS colorectal cancer cells – Pankaj Deo cancer cells – Alissa Weaver – Jessica Abner 09:15 OS19.04 – Nasal microbiota modifies OS20.04 – Extracellular vesicles limit OS21.04 – Hypoxia-driven changes the effects of particulate air pollution invasive potential of fibroblasts in in EV composition are autography- on plasma extracellular vesicles an autocrine mechanism mediated dependent – Jacopo Mariani by EVs microRNA – Marijke I. Zonneveld – Paulina Podszywalow-Bartnicka 09:30 OS19.05 – Amplifying host innate OS20.05 – The Impact of 3D cellular OS21.05 – Extracellular vesicles from immunity through modulation of p38, architecture in the microRNA and fibroblasts undergoing oncogene Jak2, and ALK activities and induction protein content of extracellular induced senescence are enriched in of macrophage differentiation and vesicles lysophosphatidic acid IL-6 dependent bacterial clearance – Sara Rocha – Krizia Sagini by exosomes released from yersinia pestis-infected monocytes – Ramin M. Hakami 09:45 OS19.06 – P2X1 purinergic blockade OS20.06 – Integrative pathway OS21.06 – Extracellular vesicles protects cells from microvesicles analysis on protein and miRNA transfer of the myddosome as the release and shiga toxin-mediated allows investigation of the role of proinflammatory signal from the toxicity extracellular vesicles in mediating Waldenstrom macroglobulinaemia – Karl Johansson the adaptive response of prostate lymphoma cells carrying cancer cells to androgen deprivation MyD88L265P mutation – Carolina Soekmadji – Mateja Mancek Keber

10:00 – 10:30 Networking Coffee Break and Exhibits Level 1 Foyer

10:30 – 11:00 PLENARY SESSION 3: EVs in Environment, Diet, and Health Auditorium Session Chairs: Hernando del Portillo; Kenneth Witwer 10:30 Introduction and Lecture: Parasitic worm EVs: From vaccines to anti-inflammatories – Alex Loukas, PhD

11:00 – 11:35 Featured Abstract Session 2 Auditorium Session Chair: Ramoroson Andriantsitohaina 11:00 Introduction 11:05 FA.03 – Live tracking of endogenous exosomes in vivo – Frederik Verweij 11:20 FA.04 – Spatio-temporal analysis of glia to neuron exosome transfer in vivo using CreERT2-reporter transgenic mouse models – Eva Maria Kramer-Albers

11:35 – 12:30 ISEV General Assembly Auditorium

12:30 – 13:45 Lunch Palau 4

12:30 – 13:45 Posters/Exhibits Level 1 Foyer

13:45 – 15:15 PARALLEL SESSIONS Room Auditorium Room 5 Room 6 SYMPOSIUM SESSION 22: SYMPOSIUM SESSION 23: SYMPOSIUM SESSION 24: Parasitic EVs: Mechanisms of EV Uptake EV-inspired Therapeutics From Basics to Translation and Biodistribution Session Chairs: Session Chairs: Session Chairs: Nobuyoshi Kosaka; Hubert Yin Amy Buck; Neta Regev-Rudzki Dave Carter; Maria Yañez-Mó 13:45 OS22.01 – Understanding host: OS23.01 – Casting a line to trailing OS24.01 – Dynamic bioreactor Pathogen interactions mediated by cells: A simple mechanism for systems for clinical scale production exosomes produced by the parasite polarizing signaling in the posterior of human amnion epithelial cells- trichomonas vaginalis lateral line primordium derived extracellular vesicles – Patricia J. Johnson – Damian E. Dalle Nogare – Gina D. Kusuma 1 4 : 0 0 OS22.02 – The role of extracellular OS23.02 – Exploring the role of OS24.02 – Development of vesicles in the modulation of exosomal surface glycans in cellular intracellular delivery system bases endothelial junctions in an in vitro uptake on extracellular vesicles derived model of cerebral malaria – Charles Williams from cells in acidic environments – Valery Combes – Ikuhiko Nakase 14:15 OS22.03 – Exploration of OS23.03 – α5β1 Integrinregulates OS24.03 – Towards extracellular extracellular vesicles from Ascaris cell adhesion to tumoral exosomes vesicles as versatile biogenic drug suum provides evidence of parasite- – Beatriz Cardenes delivery system: Loading method host cross talk by facilitated fusion with liposomes – Peter Nejsum of tunable membrane and inner composition – Max Piffoux 14:30 OS22.04 – Discovery of P. Vivax OS23.04 – A system of cytokines OS24.04 – Allogenicity boosts proteins in plasma derived encapsuated in extracellular vesicles exosome-induced antigen-specific exosomes from malaria-infected – Leonid Margolis humoral and cellular immunity and liver-chimeric (huHep) FRG mice mediate long-term memory in vivo – Melisa Gualdrón-Lopez – Susanne Gabrielsson

14:45 OS22.05 – Development of human OS23.05 – Plug-and-play decoration OS24.05 – RNA nanoparticle reticulocyte-derived exosomes as a of isolated EVs with nanobodies orientation to control ligand display new vaccine delivery platform against improves their cell-specific on exosomes for cancer regression plasmodium vivax malaria interactions – Daniel W. Binzel – Miriam Diaz-Varela – Sander A.A. Kooijmans 15:00 OS22.06 – Secreted extracellular OS23.06 – TGF beta-1 on extracellular OS24.06 – Mesenchymal stem vesicles from the hookworm-like vesicle surface: Scratching the surface cell-derived extracellular vesicles nematode nippostrongylus brasiliensis for orientation, origin and function delivered in a thermosensitive gel are prevents inducible colitis in mice – Ganesh V. Shelke effective healing mediators in porcine – Ramon M. Eichenberger and murine models of digestive fistula – Max Piffoux

15:15 – 15:45 Networking Coffee Break and Exhibits Level 1 Foyer

15:45 – 17:15 PARALLEL SESSIONS Room Auditorium Room 5 Room 6 SYMPOSIUM SESSION 25: SYMPOSIUM SESSION 26: SYMPOSIUM SESSION 27: EVs and Viral Infections EV-based Non-cancer Biomarkers OMICS Applied to EVs Session Chairs: Session Chairs: Session Chairs: Leonid Margolis; Alissa Weaver Carolina Soekmadji; Hidetoshi Tahara Alicia Llorente; Suresh Mathivanan 15:45 OS25.01 – Human immunodeficiency OS26.01 – Extracellular vesicle OS27.01 – A systems biology virus Tat-mediated synaptic biomarkers predict response to approach on pericardial fluid alterations - platelet derived growth experimental treatment in a clinical exosomes unravels relationships factor as a therapeutic strategy trial in Parkinson’s Disease between miRs, proteins and – Shilpa Buch – Dimitrios Kapogiannis metabolites that may play a potential role in promoting heart ischemia in diabetic patients – Costanza Emanueli 16:00 OS25.02 – Exosomal release of the OS26.02 – Validation of human OS27.02 – Quantitative proteomics human cytomegalovirus-encoded cerebrospinal fluid microRNAs as of transforming growth factor beta chemokine receptor US28 biomarkers for Alzheimer's Disease receptor Type 2- primed exosomes – Maarten P. Bebelman – Julie Saugstad derived from DNA mismatch repair- deficient colorectal tumor cells – Fabia Fricke 16:15 OS25.03 – JC polyomavirus uses OS26.03 – Identification of OS27.03 – Proteomic signature of extracellular vesicles to infect microRNAs from extracellular circulating extracellular vesicles in target cells vesicles as potential biomarkers for dilated cardiomyopathy – Jenna Morris-Love frontotemporal dementia – Ana Gámez-Valero – Laura Cervera-Carles 16:30 OS25.04 – Extracellular vesicles OS26.04 – On-chip detection, sizing OS27.04 – Tracking and capturing of released by zika infected cells carry and proteomics of extracellular bioorthogonal labeled RNA carried viral components: Implications vesicles by extracellular vesicles during for viral dissemination and – Celine Elie-caille maternal-embryo communication pathogenesis – Masoumeh Es-haghi – Armando Menezes-Neto

To accommodate author/presenter requests and/or cancellations, the presentation numbering may be out of sequence. Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 30 Program SATURDAY, 5 MAY 2018

16:45 OS25.05 – Microglia respond to OS26.05 Circulating macrophage- OS27.05 M. Tuberculosis HIV-1 protein Nef expression by derived extracellular vesicles predict immunomodulatory lipids, beyond releasing distinct extracellular post-operative myocardial infarction the wall. Global lipidomic analysis of vesicle population – Wade T. Rogers vesicles released by the bacillus and – Metka Lenassi by infected host cells – Emilie Layre 17:00 OS25.06 – Identifying novel OS26.06 Mass spectrometry OS27.06 ExRNA atlas analysis cellular components specifically analysis of urinary extracellular provides an exRNA census and incorporated into HIV versus vesicles recovered in the low reveals six types of vesicular and exosomes and other small EVs centrifugation pellet after elimination non-vesicular exRNA carrier profiles – Lorena Martin-Jaular of uromodulin by tris (2-carboxyethyl) detectable across human body fluids phosphine hydrochloride – Oscar D. Murillo – Luca Musante

5:15 pm – 6:30 pm Saturday Poster Session 3 (PS) Level 1 Foyer PS01: EVs in Tissue Injury and Repair PS07: EVs in Tumor Metastasis Session Chairs: Elizebet Ligeti; Magdalena Lorenowicz Session Chairs: Takahiro Ochiya; Carla Oliveira PS02: EV Engineering and Sorting of Cargo in EVs PS08: EVs as Cancer Biomarkers Session Chairs: Dave Carter; Gregory Lavieu Session Chairs: Irina Nazarenko; Yiyao Huang PS03: EV Biogenesis and Uptake PS09: Novel Developments in EV Characterization Session Chairs: Ana Gradilla; Frederick Verweij Session Chairs: Wojiec Chrzanowski; Miriam Diaz PS04: Novel Developments in EV Isolation LBS07: Repair and Signalling Session Chairs: Tom Driedonks; Louise Laurent Session Chairs: Geoffrey DeCouto; Costanza Emanueli PS05: EVs in the Nervous System (Neuronal Network, LBS08: Biogenesis Blood-Brain-Barrier) Session Chairs: Susanne Gabrielsson; Malene Joergensen Session Chairs: Dimitrios Kapogiannis; Javier Romero LBS09: Cancer II PS06: EVs in Metabolic Diseases and Aging Session Chairs: Valentina Minciacchi; Javier Sotillo Session Chairs: Nicole Noren Hooten; Ryou-u Takahashi

18:30 – 20:00 Meet the Expert Session Auditorium Biomarkers on EVs Session Chair: Andrew Hill 18:30 EVs biomarkers discovery and detection strategy – Lei Zheng, Southern Medical University, China 18:50 EV-mediated biomarkers for early detection of cancer – Takahiro Ochiya, National Cancer Center Research Institute, Japan 19:10 EV-related biomarkers in peritoneal dialysis – Francesc E. Borràs, Institut German Trias I Pujol, Spain 19:30 Searching for prostate cancer biomarkers in urinary exosomes – Alicia Llorente, University of Oslo, Norway

18:30 – 20:00 Meet the Expert Session Room 5 EVs in Neglected Tropical Diseases Session Chairs: Igor Almeida; Carmen Fernandez-Becerra 18:30 EVs in flatworm infections – Antonio Marcilla, Universitat de València, Spain 18:50 EVs and exRNA in parasitic nematodes: At the host interface – Amy Buck, University of Edinburgh, United Kingdom 19:10 Vesicles in protozoa infection – Ana Claudia Torrecilhas, UNIFESP, Brazil 19:30 Non-Enveloped LRV1 virus exploits the leishmania exosomal pathway for its propagation – Martin Olivier, McGill University, Canada Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 31 Program SATURDAY, 5 MAY 2018

18:30 – 20:00 Meet the Expert Session Room 6 Can Research on EVs Accelerate Clinical Impact in Leukemia? (Supported by the Fundació Josep Carreras) Session Chairs: Evaristo Feliu Frasnedo; Theresa Whiteside 18:30 Cell-cell communication between immune and tumor cells. implications for tumor cell resistance mechanisms – Beatriz Martin Antonio, Josep Carreras Leukaemia Research Institute, University of Barcelona, Spain 18:50 Leukemia exosomes and their role in defining tumor microenvironment – Stefania Raimondo, University of Palermo, Italy 19:10 Leukemia EVs: Impact on early hematopoiesis and diagnostic potentials – Bernd Geibel, University Hospital Essen, Germany 19:30 Yin and yang of extracellular vesicles in leukemia – Peter Quesenberry, Brown University, United States 20:15 – 23:59 Networking EVent Cupula at Arenas de Barcelona

To accommodate author/presenter requests and/or cancellations, the presentation numbering may be out of sequence. Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 32 Program

SUNDAY, 6 MAY 2018

08:00 – 14:00 Registration and Information Desk Level 1 Foyer

09:00 –10:30 PARALLEL SESSIONS Room Auditorium Room 5 Room 6 SYMPOSIUM SESSION 28: SYMPOSIUM SESSION 29: SYMPOSIUM SESSION 30: Late Breaking Session Late Breaking Session Late Breaking Session Session Chair: Session Chairs: Session Chair: Dolores Di Vizio Tang Long Shen Lei Zheng 09:00 LB01.01 – Blood microvesicles LB02.01 – On-chip liquid biopsy: LB03.01 – Regulation of exosome derived from neurovascular Progress in isolation of exosomes secretion via the intricate tuning of network correlate with amyloid-β and their RNA sequencing for multivesicular endosome transport deposition in the brains of prognosis of prostate cancer towards the plasma membrane postmenopausal women – Navneet Dogra – Guillaume van Niel – Muthuvel Jayachandran 09:15 LB01.02 – Graphene-oxide LB02.02 – Tissue-tropism, parasite LB03.02 – Naturally and targeted quenching-based molecular beacon cargo and signaling of spleen engineered DNA cargo in bacterial imaging for exosome-mediated fibroblasts by plasma-derived extracellular vesicles control rates transfer of neurogenic miRNA on exosomes from vivax malaria of interspecies horizontal gene microfluidic platform infections exchange and can be regulated by – Hyun Jeong Oh – Haruka Toda environmental cues – Frances Tran 09:30 LB01.03 – Menadione driven LB02.03 – Alterations in platelet LB03.03 – Harnessing extracellular oxidative stress related microvesicle membranes and formation of lipid vesicles from human red blood cells shedding associates with resilience mediators during storage for gene therapies against cancer to hypoxia in a neuronal ischaemia – Sami Valkonen – Minh TN Le model – Emma Buzzard 09:45 LB01.04 – Unravelling the role LB02.04 – Validation of purification LB03.04 – Exosomes as drug of extracellular small heat shock methods for extracellular vesicles delivery vehicles for therapeutic proteins in neuroinflammation – Jacopo Zini proteins to the brain – Joy Irobi – Elena V Batrakova

10:00 – 10:30 Networking Coffee Break and Exhibits Level 1 Foyer

10:30 – 11:00 PLENARY SESSION 4: Tissue Microenvironment and Architecture Auditorium Session Chairs: Eva Maria Kramer-Albers; Hector Peinado 10:30 Introduction and Lecture: How does a linear sequence of DNA become a 3-dimensional tissue and why cancer cells forget! – Mina Bissell, PhD

11:00 – 12:30 Featured Abstracts Sunday Auditorium Session Chair: Rienk Nieuwland 11:00 Introduction 11:05 FA3.01 – Recombinant extracellular vesicles: Biological reference material to standardize extracellular vesicle research – Edward Geeurickx 11:20 FA3.02 – A genome-wide CRISPR screen using barcoded-microRNAs enables systematic interrogation of extracellular vesicle biology – Albert Lu To accommodate author/presenter requests and/or cancellations, the presentation numbering may be out of sequence. Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 33 Program SUNDAY, 6 MAY 2018

11:35 FA3.03 – Systematic methodological evaluation of a multiplex bead-based flow cytometry assay for detection of extracellular vesicle surface signatures – André Görgens 11:50 FA3.04 – miRNAs expressed in brain and serum extracellular vesicles act as indicators of preclinical and clinical Prion Disease – Lesley Cheng 12:05 FA3.05 – Non-invasive brain delivery with hybrid extracellular vesicles (EVs) for therapy of Machado-Joseph Disease (MJD) – Patricia Albuquerque

12:30 – 12:50 Wrap Up Sessions Auditorium 12:30 Basic Science – Alissa Weaver 12:40 Clinical Aspect – J. Brian Byrd

12:50 – 13:30 Awards Ceremony and Closing Remarks Auditorium

Research Methods for Extracellular RNA exRNA – Workshop of The Extracellular RNA Communication Consortium (NIH) Session Chair: Dr. Roger P. Alexander Room 3 This event is open for all Educational Day and ISEV2018 attendees!

18:00 Opening Remarks – Roger P. Alexander, PhD 18:00 The biology of exRNAs in CSF OR Overview of biofluid RNA profiles – Julie Saugstad, PhD, OHSU, USA 18:20 Standardizing exRNA isolation protocols – Louise Laurent, PhD, UCSD, USA 18:40 Integrative analysis of exRNA datasets in the exRNA Atlas – Joel Rozowsky, PhD, Yale University 19:10 The biology of exRNA in saliva / Unique requirements for analysis of salivary exRNA data – David Wong, PhD, UCLA 19:30 Closing Remarks

Emerging Trends in EVs Associated with HIV & Drug Abuse Session Chairs: Dr. Shilpa Buch & Dr. Kenneth Witwer Room 4 This event is open for all Educational Day and ISEV2018 attendees!

18:00 Opening Remarks – Shilpa Buch, PhD 18:10 Human Immunodeficiency Virus Tat-Mediated Synaptic Alterations - Platelet Derived Growth Factor as a Therapeutic Strategy – Shilpa Buch, PhD, Professor and Vice Chair for Research, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center 18:25 Cigarette Smoke, EVs and HIV disease – Kenneth Witwer, PhD, Associate Professor, Johns Hopkins University 18:40 Plasma Neuron-derived Exosomes in HIV: Window into Neuronal Dysfunction – Lynn Pulliam, MS, PhD, Professor, Chief, Microbiology (VAMC) Laboratory Medicine, Departments of Pathology & Laboratory Medicine, University of California-San Francisco 18:55 Intranasal Delivery of Lncrna-Cox2 Sirna Loaded Exosomes as a Therapeutic Strategy for Restoring Lipopolysaccharide and Morphine Mediated Functional Impairment of Microglia – Guoku Hu, PhD, Assistant Professor, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center 19:10 Effects of Methamphetamine on EV Biogenesis and microRNA Cargo – Sowmya V. Yelamanchili, PhD, Assistant Professor, Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center 19:25 Novel communications in HIV-1/cocaine associated noninfectious communications – Navneet Kaur Dhillon, PhD, Professor, Pulmonary and Critical Care 19:40 Potential Utility Of Engineered Exosomes for Targeted Delivery of Therapeutics – Archana Gupta, PhD, Scientist II & Science and Technology Communication Specialist, Systems Biosciences 19:55 EVs carry HIV Env and Facilitate HIV Infection of Human Lymphoid Tissue – Anush Arakelyan, PhD, Staff Scientist. National Institutes of Health 20:10 Separating HIV from exosomes: not such an easy task! – Lorena Martin-Jaular, PhD, Senior post-doc Institute Curie 20:25 Closing Remarks

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 35 Young Research Development Career on the EV Field FRIDAY, 4 MAY, 12:40–13:40 Room 3

CHAIR: Delphine Muriaux CNRS research director currently at the head of the Cemipai (www.cemipai.cnrs.fr) and a principal investigator at IRIM (Institut de Recherche en Infectiologie de Montpellier) CNRS Montpellier, France. PhD in molecular and cellular biology from Paris 6 University (France) and postdoc/research assistant training in virology at the NIH (USA). Her team (“membrane domains and viral assembly” at IRIM CNRS) and research focus are more dedicated to the comprehension of molecular and cellular mechanisms of RNA enveloped viruses, virus-like particles and extracellular vesicles production.

SPEAKERS: Neta Regev-Rudzki BSc in chemistry, an MSc in biochemistry, PhD in microbiology and cell biology (2010), all at the Hebrew University in Jerusalem, Israel. Postdoc at Walter and Eliza Hall Institute of Medical Research (WEHI) in Melbourne, Australia. Discovered that malaria parasites, which live inside red blood cells, transfer episomal genes between them via EVs. October 2014, faculty of Department of Biomolecular Sciences at Weizmann Institute of Science (Israel). Her lab is focused on studying modes of cell-cell communication developed by lethal human pathogens, e.g. malaria parasites.

Davide Zocco Currently the Head of Precision Medicine at Exosomics Siena where he leads development of EV- DNA and EV-RNA targeted solutions and overseas clinical testing of assay prototypes. Davide is part of strategic planning and scouting for new technologies, as well as, internal IP strategy and pre-market launch of RUO and diagnostic kits. PhD from University College Dublin (Ireland) and a postdoctoral research fellow at Harvard Medical School (USA).

Elisa Lazaro-Ibañez MSc degree in Biotechnology in 2010 from the Polytechnic University of Valencia, Spain. Postdoc at CIPF (Valencia, Spain), focused on nucleic acid content of EVs from Glioblastoma Multiforme. Ph.D. at Helsinki University in 2017. Visiting researcher at the University of Gothenburg, Sweden. After having studied EVs in biomarker discovery for over 7 years, she moved to industry where she is currently investigating the use of EVs as drug delivery agents. Since, 2017 is postdoctoral researcher in Discovery Sciences, Discovery Biology team in the IMED Biotech Unit of AstraZeneca in Gothenburg, Sweden.

Johan Skog PhD in gene therapy and virology from Umea University, Sweden. Postdoc at Massachusetts General Hospital/Harvard Medical School where he was studying RNA in tumor stem cells. He had found that primary tumors shed RNA-containing EVs. Founder scientist and Chief Scientific Officer of the company Exosome Diagnostics Inc. that was formed in 2008 and has since then raised over $108 million and employs over 80 people. He continues to pioneer critical advancements in diagnostics and expand the field of exosome biology and has >138 applications/divisionals/or granted patents in the exosome field.

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 36 3D VIRTUAL ANIMATION ON Extracellular Vesicles Thursday, 3 May 13:00–13:20 Projection 2D Auditorium Friday, 4 May 08:30-13:45 Virtual 3D Room 1 Extracellular Vesicles The Cell’s Secret Messengers

Presenter: Virtual 3D Dave Carter Biomedical Science, Oxford Brookes University

Acknowledgments: Education Arkitek Committee studios

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 37

Meet the National Societies and Outreach Strategies Thursday, 3 May 2018 18:30 – 20:00

Room 6

Session Chair: Isabel Guerrero

18:30 Welcome Isabel Guerrero 18:35 Dutch Society Marca Wauben 18:40 German Society Irina Nazarenko 18:45 French Society Guillaume van Neil 18:50 UKEV Forum Dave Carter 18:55 GEIVEX, Spanish Society Hernando del Portillo 19:00 Portuguese EV Branch Carla Oliveira 19:05 Slovenian Network on EVs Metka Lenassi 19:10 Brazilian Society Ana Claudia Torrecilhas 19:15 Australasia Extracellular Vesicles Suresh Mathivanan 19:20 Chinese Society Lei Zheng 19:25 Japanese Society Takahiro Ochiya (JSEV President) 19:30 Korea Society for Extracellular Vesicles (KSEV) Kwang Pyo Kim 19:35 TSEV (Taiwan Society for Extracellular Vesicles) Tang-Long Shen 19:40 Expertise for funding EVs networks Cherie Blenkiron 19:45 H2020 Cost and ITN actions Lorraine O'Driscoll 19:50 Q&A

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 38 Abstract Directory Alpha Last Name First Name Poster # Poster Title A Abramov Alexandr PT06.04 Cells interactions and cells modifications via exosomes Abramowicz Agata LBT03.03 Proteomic analysis of exosomes released from irradiated head and neck cancer cells Identification of human melanoma biomarkers by comparative exoproteoma analysis of Agüera-Lorente Andrea PT05.06 melanocytes and melanoma cells Catching the Hedgehog: Unraveling Hedgehog secretion during filopodia-mediated Aguilar Gustavo LBS08.07 transport Al Jallad Hadil PT06.05 Characterization of Osteoblast -derived Exosomes of Distinct Emryonic Origin Characterization of human plasma extracellular vesicles and their role in aging-related Alberro Ainhoa PF04.06 immunosenescence and immune response miRNA profiling of circulating EVs in Myalgic Encephalomyelitis/Chronic Fatigue Almenar-Pérez Eloy PF04.05 Syndrome (ME/CFS) Modulation of exosome content through metabolic inhibitor treatment for antiviral Alonzi Rhea PF09.12 outcomes To study anti tau antibody loading and neuronal uptake efficiency of Human bone marrow Amini Azadeh PF07.03 mesenchymal stem cells derived extracellular vesicles Ando Mitsuru PS02.08 Preparation and function of CD9-integrated proteoliposomes Extracellular vesicles shedding in response to chemotherapy in melanoma promotes tumor Andrade Luciana PS08.19 growth after temozolomide treatment André-Grégoire Gwennan PT03.02 Characterization of Extracellular Vesicles from Glioblastoma Brain Tumours Critical role of Rap1 in triggering the effects of microparticles from metabolic syndrome Andriantsitohaina Ramaroson PS06.02 patients on vascular smooth muscle cell functions Immunoproteomic characterization of Outer Membrane Vesicles from high-producing Antenucci Fabio PF04.04 Actinobacillus pleuropneumoniae Antich-Rosselló Miquel PS01.17 Human platelet derived exosomes induce chondrogenic differentiation Aqil Farrukh PT07.11 Milk exosomes – Intranasal versus oral delivery of exosomes and exosomal-curcumin Human cytomegalovirus-infected cells release extracellular vesicles that carry viral Arakelyan Anush PF09.11 surface proteins Archer Fabienne PF09.10 Oncogenic retroviral protein is conveyed by extracellular vesicles in ovine lung cancer Aricha Revital LBF06.08 Development of MSC-NTF cell exosomes for the treatment of neurodegenerative diseases Rheumatoid factor is detected on circulating extracellular vesicles in a subpopulation of Arntz Onno PT09.03 rheumatoid arthritis patients with a more severe disease phenotype Artuyants Anastasiia PF09.07 Characterization of extracellular vesicles produced by vaginal microorganisms A novel two-step EV isolation from plasma using size exclusion chromatography and Askeland Anders PF06.13 antibody-mediated removal of lipoproteins Human bone marrow mesenchymal stem cells derived extracellular vesicles in vitro Assar Kashani Sara PS06.04 characterization and cytotoxicity on rat islet cells Auber Martin PF06.05 Serum-free media supplements carry miRNAs that co-purify with extracellular vesicles Ayers Lisa PT08.12 Microvesicle release during exercise-induced cardiac stress in young adult hypertension Aziz Mohammad LBS08.04 Evidence for selective mRNA sorting into cancer exosomes Treatment with intravenous immunoglobulin increases the level of small EVs in plasma of B Baek Rikke PT02.14 pregnant women with recurrent spontaneous abortions Balbi Carolina PF08.10 hAFS-EV cardioactive potential for myocardial regeneration Balint Stefan LBS07.09 T-cell synaptic ectosomes relay signals through microcluster transfer Extrusion of mesenchymal stromal cells produces EV-like vesicles that attenuate allergic Bandeira Elga PT07.01 airway inflammation Banfai Krisztina PS06.11 Role of Wnt4 exosomes in thymic aging Urinary extracellular vesicles in diabetic kidney disease: validation of preferable Barreiro Karina PS04.04 preparative techniques Bautista-Moreno Filiberto PF06.01 Characterization of RNA contained in highly purified exosomes from fetal bovine serum Bedina Zavec Apolonija LBF04.03 The response of the cells to toxin Listeriolysin O and its mutants Berganza Jesus PS09.03 Development of lateral flow test for detection of exosomes biomarkers in urine samples

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 39 Abstract Directory Alpha Last Name First Name Poster # Poster Title Plasma-derived extracellular vesicles contain mutant SOD1 in hSOD1G93A Berrone Elena PF07.08 transgenic swine Bhattacharyya Suvendra PF04.16 HuR driven extracellular export of miRNA in mammalian hepatic and immune cells Specific capture and analysis of tumor exosomes in plasma is a promising liquid biopsy Bianciardi Laura PF01.18 approach for comprehensive detection of actionable genomic alterations in prostate cancer patients Cardiac dysfunction after myocardial infarction: role of pro-inflammatory extracellular Biemmi Vanessa PT08.07 vesicles. Ischemia-related conditions induce secretion of miR-21-5p-containing extracellular Bister Nea PF07.13 vesicles that alter microglial activation Exosomal miR-486-5p, miR-181a-5p, and miR-30d-5p from hypoxic tumor cells are Bjørnetrø Tonje PS08.13 candidate circulating markers of high-risk rectal cancer Decoding the role of circulating extracellular vesicles in Chronic Fatigue Syndrome/Myalgic Blanco-López Maria del Carmen PT09.08 Encephalomyelitis: an exploratory pilot study Blenkiron Cherie PT02.07 Effects of temperature on placental extracellular vesicle characteristics S-palmitoylation is a post-translational modification of Alix that regulates its interaction Bongiovanni Antonella PS03.03 with the CD9 tetraspanin Borgovan Theo PF02.15 Initiation of Leukemia Phenotype via Extracellular Vesicles Isolation of extracellular vesicles by gel filtration: A comparison of two commonly used Borosch Sebastian PS04.18 protocols The somewhat forgotten role of isotype control antibodies in selecting and validating Botha Jaco PF01.16 phenotype markers and antibody panels for EV characterisation Small-particle flow cytometry: A new frontier in detection and characterisation of Botha Jaco PS09.13 extracellular vesicles in liquid biopsies A Mixed Lymphocyte Reaction as a Functional Assay for Extracellular Vesicles of Different Bremer Michel PF04.12 Origins Imaging flow cytometry: A potent method to identify distinct subpopulations of small Bremer Michel PS09.07 extracellular vesicles Bremer Michel PT05.18 Optimization of Storage Conditions for Extracellular Vesicles Brittain George IP 01 A nano- and microparticle mix for CytoFLEX size standardization Brittain George IP 02 A prototype CytoFLEX for high-sensitivity, multiparametric nanoparticle analysis Analysis of nuclear and mitochondrial DNA ratio in human melanoma cell line derived Brodesser Daniela PF02.10 vesicles Optimization of Flow Cytometer Settings with Fluorescently-Tagged Retrovirus for the Burger Dylan PF01.09 Analysis of Extracellular Vesicles by Nanscale Flow Cytometry Burger Dylan PF01.17 Effect of hemodialysis on circulating submicron particle levels Buschmann Dominik PF08.03 Can vesicular microRNAs predict negative perioperative outcomes in cardiac surgery? A comparison of extracellular vesicle subtypes released by triple-negative breast cancer C Cabral Joana PF02.11 and non-tumorigenic breast epithelial cells Proteomic characterization and anti-inflammatory effect of primed canine adipose Cajon Pauline PF03.18 mesenchymal stem cell conditioned medium Campos Silva Carmen PF02.08 New and improved methods for characterization of tumour-specific markers in exosomes Characterization and proteomic profile of extracellular vesicles from peritoneal dialysis Carreras-Planella Laura PF05.04 efflux Castellanos-Rizaldos Elena PF02.20 Highly sensitive detection of IDH1 R132H mutations in plasma of glioma patients Pattern of secretion in vitro of microvesicles and exosomes in equine mesenchymal stem Castro Fidel Ovidio PF03.11 cells derived from the same animals but from different tissues miR-296-5p and PDGF-BB in CD31EV cargo: novel biomarkers of vascular smooth muscle Cavallari Claudia PS06.09 cell dysfunction in diabetes Cereda Cristina PF07.07 Increased size of extracellular vesicles in Amyotrophic Lateral Sclerosis Cereda Cristina PS06.08 Microvesicles as novel biomarkers of frailty Chang Clare (Chi-Chih) PT06.07 Extracellular vesicular miRNAs in osteoblastogenesis

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 40 Abstract Directory Alpha Last Name First Name Poster # Poster Title In vitro characterization of cardiac extracellular vesicles involved in transport of the Charrier Henri PT03.07 circulating biomarkers of heart failure miR-21-5p, miR-23a-3p and miR-222-3p Protein profiling of extracellular vesicles from the oviductal fluid of sows before and after Chen Shuai PT02.13 ovulation Proteomics Discovery of Novel Plasma Exosome Biomarkers for Early Detection of Patients Cheow Esther PT05.01 at Risk for Non-small Cell Lung Cancer (NSCLC) An increased level of CD41-positive extracellular vesicles recovered by 100,000×g Chiang Chun-yi PF05.08 centrifugation from stimulated platelets Semi-quantitation and characterization of serum-derived exosomes in coronary artery Chiang Dapi PS04.19 disease by glycan recognition bead, EXÖBead Chiari Marcella PS09.12 High-visibility detection of exosomes by interferometric reflectance imaging Plasma Exosome Count is Correlated with Grade of Lung Cancer Stages; comparison Choi Byeong Hyeon LBT02.04 between Pulmonary Vein versus Peripheral Vein T-cell-derived exosomes are potential biomarkers or therapeutic targets for autoimmune Chuang Huai-Chia PT09.07 diseases Bioinformatics analysis of metabolites present in urinary exosomes identify metabolic Clos-Garcia Marc PT05.17 pathways altered in prostate cancer Cocks Alex PF02.16 Endocytosis and intracellular trafficking of prostate cancer exosomes Exosomes as biomarkers for identification quantitation and stratification of Chronic Cohen Sapir PT05.08 Lymphocytic Leukemia Protein signatures in exosome-like vesicles of uterine aspirates improve the diagnosis and Colas Eva PT05.12 stratification of endometrial cancer patients Cooks Tomer PT04.21 Mutant p53 controls tumor microenvironment via extracellular vesicles Mesenchymal Stromal Cell Derived Extracellular Vesicles Show Distinct Chondrogenesis Crossland Rachel PF03.13 MicroRNA Expression Profiles from their Parental Cells Cvjetkovic Aleksander LBT02.09 Proteomic analysis of tumor tissue resident EVs in Breast Cancer Arginase-1-containing exosomes induce suppression of antitumor in vitro and in vivo Czystowska-Kuzmicz Malgorzata PT04.16 immune response D da Silva Lira Filho Alonso PT01.01 GP63-Enriched Leishmania Exosomes Concur to Cutaneous Leishmaniasis Development. Small extracellular vesicles from follicular fluid of growing follicles from bovine are da Silveira Juliano PT02.03 enriched of precursor miRNAs Co-localization, Counting, and Size Characterization of Single Exosomes using a Direct Daaboul George PS09.20 from Sample Surface Capture Based Imaging Technique Angiogenic Activity of Menstrual Blood Mesenchymal Stromal Cell Exosomes in Wound Dalirfardouei Razieh LBS07.17 Healing Process of Diabetic Mice Extracellular vesicles isolated from cardiosphere-derived cells and mesenchymal stem cells de Couto Geoffrey LBS07.16 elicit distinct immunomodulatory properties in vitro and in vivo De Hoff Peter PF05.12 Gestational age prediction using maternal serum and placental miRNA expression Unveiling the role of EV-mediated vascular cell:cell communication in human pulmonary De la Cuesta Fernando PT08.06 hypertension Comparison of generic fluorescent dyes for detection of extracellular vesicles by flow de Rond Leonie PF01.01 cytometry De Santana Eliezer LBS07.14 The use of exosomes as an important tool to kidney recellularization Debets Donna Olivia PT03.05 Proteomic analysis of breast cancer-derived extracellular vesicles Tumor-derived exosomes contribute to a pro-tumorigenic inflammatory DeClerck Yves PT04.05 microenvironment in cancer DeCorwin-Martin Philippe PS09.15 Nanoarray for single exosome-like extracellular vesicle proteomics Cell communication via microRNA exchange between endothelial and tumour cells during Dederen Stella PT04.06 anti-cancer neoadjuvant therapy Alterations in the miRNA cargo of HIV-infected macrophage-derived extracellular vesicles Dhillon Navneet LBF05.04 promote pulmonary smooth muscle proliferation Repeatable, high-purity isolation of urinary extracellular vesicles for uro-oncological Dhondt Bert PS04.02 biomarker studies

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 41 Abstract Directory Alpha Last Name First Name Poster # Poster Title Liquid Biopsy on a Chip: Microfluidic Isolation and RNAseq analyses of cancer derived Dogra Navneet IP 08 exosomes Sequencing and reproducibility analyses of small RNA extracted from prostate cancer Dogra Navneet PS04.10 exosomes isolated using nanoDLD chip technology. Extracellular vesicles induce fibroblasts metalloproteinases expression in thyroid tumor Donadio Ana PT04.08 microenvironment Cathepsin B cysteine protease of L. donovani: role in the modulation of parasitic exosomal dos Santos Meira Camila PT01.10 proteins and TGF-β1 and arginase activities in macrophages Endogenous and exogenous loading of extracellular vesicles for therapeutic delivery of the Downie Laura PT07.14 renin-angiotensin system peptide angiotensin-(1-7) in cardiomyocyte hypertrophy Comparison of Fetal Calf Serum EV-depletion protocols indicates differences in depletion Driedonks Tom PF06.02 efficiency of miRNAs and other RNA classes Microvesicles induced in hyperglycemic conditions regulate endothelial cell stiffness and Drożdż Anna PS01.12 cell shape fluctuations Duan Wei PF06.03 How good is the gold standard-the impact of methods on exosome function study Emmanouilidi Aikaterini PT05.05 Exploiting lipidomics to unravel novel biomarkers for pancreatic cancer Ende Stefanie LBT02.11 Finding the Needle in the Haystack – Prostate Cancer Diagnostics by Liquid Biopsy Enderle Daniel PF01.08 Physical activity as pre-analytical parameter for liquid biopsies EVs from Wistar Kyoto and spontaneously hypertensive rats have differential vasodilatory Erdbrügger Uta PT08.01 effects on resistance arteries Esteves Sofia PF05.07 Use of Leishmania promastigote EVs in serological diagnosis of leishmaniasis Functionality of bone marrow mesenchymal stem cell-derived extracellular vesicles on F Fafian Labora Juan Antonio PT06.08 self-renewal Fan Hongkuan PT09.13 Endothelial Progenitor Cell Exosomes Improve the Outcome of a Murine Model of Sepsis Fang Hui PT09.06 Proinflammatory role of blister fluid-derived exosomes in bullous pemphigoid Characterization of extracellular vesicles produced by single human embryos at early Fazeli Alireza PT02.02 stages of development Feng Ye PF05.03 Urinary exosomes and the packing CCL-2 mRNA as biomarkers of IgA Nephropathy Fernández Rodríguez Amanda PT03.08 Liver-specific miRNAs are detected in exosomes from HIV/HCV patients Immunomodulatory activity of clinical grade mesenchymal stem cell-derived extracellular Fierabracci Alessandra PF04.18 vesicles on human NK cell activities Optimizing loading and expression of HChrR6 mRNA in extracellular vesicles (EVs) for side Forterre Alexis PT07.05 effect-free prodrug-mediated treatment of HER2+ve breast cancer Cell-free regenerative medicine: use of human platelet-derived extracellular vesicles to Forteza-Genestra Maria Antonia PS01.18 induce pre-osteoblast differentiation Microfluidic resistive pulse sensing (MRPS) validated as a rapid and practical method for Fraikin Jean-Luc IP 09 evaluating EV enrichment techniques Analysis of exosome concentration in blastocyst culture media by microfluidic resistive Fraikin Jean-Luc PT02.09 pulse sensing correlates with embryo implantation capacity: a pilot study. Frias Jorge LBS07.07 Identification of exosomes in the infective stage of entomopathogenic nematodes Immunophenotyping extracellular vesicles by flow cytometry using CCD-based imaging Friend Sherree PS09.16 technology Increased amounts of cancer-related membrane molecules in extracellular vesicles Furukawa Koichi PF02.13 secreted from ganglioside-enriched cancer cell lines Biodistribution and proteomics analysis of plasma-derived EVs from Fasciola hepatica G Galiano Alicia PT01.06 infections T-lymphocytes are not involved in the preventive effect of fasciola hepatica EVs in DSS- Galiano Alicia PT01.07 induced acute ulcerative colitis MicroRNA signature from plasma-derived EVs for Dementia with Lewy bodies as promising Gamez-valero Ana PF05.01 non-invasive biomarker Garai Kitti PF04.02 Correlation of exosomal miRNA- and anthropometric profile of an active lifestyle Monocyte-derived Extracellular Vesicles involve Mesenchymal Stem/Stromal Cells into Gebraad Arjen PF03.15 Tissue Remodeling

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 42 Abstract Directory Alpha Last Name First Name Poster # Poster Title MicroRNA-Containing Microvesicles of Healthy Origins: A Potential Tool for the Therapy of Georgescu Adriana LBS07.10 Atherosclerosis Geraci Fabiana PF03.01 Mesoangioblast derived extracellular vesicles have paracrine effects on different cell types Systems biology analysis reveals that several common diseases are associated with genes Gézsi András LBS08.01 involved in the biogenesis of extracellular vesicles Ghayad Sandra PF02.05 Rhabdomyosarcoma exosome proteomics yield functional role for extracellular vesicles Ex vivo expansion of umbilical cord blood-derived hematopoietic stem cells using Ghebes Corina PT06.09 extracellular vesicles of bone marrow stromal origin Ghoroghi Shima PS07.04 Contribution of Ral GTPases dependent extracellular vesicles to lung metastasis Capture and label-free detection extracellular vesicles on gold-nano-island based Ghosh Anirban PS04.13 microfluidic Lab-on-a-CHIP device using synthetic-peptide Vn96 Chitosan, a non-toxic polysaccharide based extracellular vesicle isolation technology*: Ghosh Anirban PS04.15 Potential for therapy and liquid biopsy applications microRNA expression profile in microvesicles released from genistein-treated immune Gimeno-Mallench Lucia PF06.20 cells. EVs isolation by SMART-SEC: analysis of isolated contaminants and fluorescent labelled Gonzalez Esperanza PS04.12 EVs Use of tumor-secreted exosomes to define new biomarkers and targets to prevent González Muñoz Teresa PS07.03 malignant peripheral nerve-sheath tumor progression Gourzones Claire PF01.05 Molecular drivers and markers of pancreatic cancer initiation and progression Gradilla Ana PT06.01 Driving Patched to the bottom: vesicular trafficking to polarize Hh reception. Extracellular vesicles (EVs) secreted by mesenchymal stem cells (MSCs) exert opposite Grassi Michele PT09.04 effects with respect to their cells of origin in mice with DSS-induced colitis Grillari Johannes PS06.12 Extracellular vesicles and their miRNA cargo in aging and age-associated diseases Groenewegen Nils LBT03.05 Comprehensive small RNA sequencing and analysis of Extracellular Vesicles The effect of remote ischemic conditioning on the physicochemical properties of Gu Tingting PF07.12 extracellular vesicles Raman spectroscopy for the molecular profiling of extracellular vesicles in Parkison’s Gualerzi Alice PF07.05 disease Guix Rafols Francesc Xavier LBF06.06 Analysis of tau in neuron-derived extracellular vesicles Guryev Oleg IP 05 Affinity Purification of Membrane Vesicles Glioblastoma stem cells induce an invasive phenotype in normal astrocytes via H Hallal Susannah PS07.15 extracellular vesicles Proteome analysis of glioma-derived extracellular vesicles isolated from neurosurgical Hallal Susannah PT05.14 aspirates provide markers for disease stage and progression Hansen Maria PS03.12 Bioavailability of bovine milk extracellular vesicles Hatzidaki Eleana PS02.02 EV-encapsulated small molecule inhibitor decreases viability in cancer cell lines Radio-detoxified endotoxin alters the protein profile of bone-marrow derived exosomes Hegyesi Hargita LBT03.06 and enhances the release of endothelial progenitor cells in local chest-irradiated mice Involvement of platelet αIIbβ3 integrin and downstream signaling pathways in release of Heinzmann Alexandra PF08.07 extracellular vesicles, CXCL4 and CCL5 Helmbrecht Clemens IP 15 Phenotyping of EVs by Multi-Wavelength Fluorescence Nanoparticle Tracking Analysis Henriksson Eva PS07.14 Heat Shock Factor 2 associates with cancer-derived extracellular vesicles Comparative analysis of extracellular vesicles from arterial and venous blood reveals only Hermann Stefanie PF08.02 minor differences in vesicle composition Pathological spread of TAR DNA binding protein 43 (TDP-43) in an induced pluripotent Hicks David PS05.03 stem cell model of dementia Higginbotham Samuel PF02.01 Probing the role of myofibroblast-derived extracellular vesicles in cancer Novel AC electrokinetic platform for rapid isolation and characterization of extracellular Hinestrosa Juan PS04.11 vesicles from NSCLC patients Comparison of non-coding RNA content of extracellular vesicles derived from Wharton’s Hoffman Andrew LBS07.05 Jelly, Amniotic, and Chorionic mesenchymal stem cells

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 43 Abstract Directory Alpha Last Name First Name Poster # Poster Title Flow cytometry analysis of platelet microparticles in cord blood: the effect of delay in Holada Karel PF01.12 sample preparation. The role of extracellular vesicles in mediating placental responses to maternal cellular Holder Beth PT02.15 stress Extracellular vesicles mediate a targeted translocation of functional inflammatory Hosseinkhani Baharak PT08.10 modulators between vascular endothelial cells and monocytes Hsieh Ching-Hua PT09.14 Exosomes with different surface markers present various exosomal content and function Lactobacillus plantarum-derived extracellular vesicles improved the quality and safety of Hsu Wei-Hsuan LBF04.04 tuna meat Intranasal delivery of lncRNA-Cox2 siRNA loaded exosomes as a therapeutic strategy for Hu Guoku PS05.06 restoring lipopolysaccharide and morphine mediated functional impairment of microglia Progress in separation of extracellular vesicles from brain tissue of human, macaque, and Huang Yiyao LBF06.01 mouse Huang Yiyao PF06.08 Purification of extracellular vesicles from plasma by heparin-coated magnetic beads Identifying potential biomarkers for lung cancer from the cancer derived exosomes using Huang Wei-Lun PS08.02 the nano gap-mode surface-enhanced Raman scattering (SERS) Plasma EV phenotype modulation in metastatic renal cell cancer patients receiving Huber Veronica LBT02.06 tyrosine kinase inhibitor Hunt Stuart PF02.18 Extracellular vesicle cargo is indicative of HPV status in oropharyngeal carcinoma I Ikuo Mariko PS08.15 Extracellular vesicles derived from senescent cells repress tumor growth via miRNAs Imawaka Naoko IP 13 Characteristics of PS-affinity method for isolation and detection of EVs Ingato Dominique PT07.10 Chemotherapeutic-loaded extracellular nano-vesicles produced via sulfhydryl blocking Bystander effect of exosomes derived from cervical adenocarcinoma cells in response to Inubushi Sachiko LBS09.01 irradiation Identification of androgen dependent glycosylations on the surface of extracellular Islam Md Khirul PT05.02 vesicles-derived from prostate cancer cell lines Ivanova Alena PT06.06 Secretion mechanisms of Wnt proteins Extracellular vesicles from metastatic medulloblastoma cell lines carry mRNAs known to J Jackson Hannah PS07.05 correlate with metastatic disease Modulation of microglia responses via mesenchymal stromal cells derived- extracellular Jaimes Yarua LBF06.05 vesicles. Excretion of urinary extracellular vesicles are not differ between apparently healthy Jayachandran Muthuvel PS01.05 postmenopausal women without and with histories of preeclampsia Differential expression of miRNAs in urinary extracellular vesicles from pregnant women Jayachandran Muthuvel PT02.04 with and without preeclampsia Extracellular Vesicles from Kaposi’s sarcoma-associated herpesvirus infected-human Jeon Hyungtaek LBF04.02 endothelial cells stimulate the type 1 interferon response. Regulation of exosome release in lung cancer cell lines by a lung cancer exosome-specific Jeong Hyesun PT05.04 protein 1 (LESP1) Remote loading of ester-based prodrugs and fluorescent labels using intravesicular Jiang Linglei PS02.07 hydrolases TGF-β1 silencing adipose stem cell-derived exosomes as a new therapeutic strategy for Jin Yinpeng PF03.14 liver fibrosis MicroRNA-19 in human adipose-derived stem cells exosomes rescuing acute liver failure Jin Yinpeng PT08.16 rats models through anti-inflammatory effect Johnson Suzanne PF02.04 Characterisation of large extracellular vesicles in paediatric medulloblastoma Extracellular vesicles derived from natural killer cells use multiple cytotoxic proteins and Jong Ambrose PT04.18 killing mechanisms to target cancer cells Jørgensen Malene PF06.17 Extraction and analysis of intact EVs collected from dried blood spots Regulation of therapeutic compounds in extracellular vesicles by 3D-organizing different Jung Sunyoung PF03.17 physical interactions between mesenchymal stem cells and culture matrices Jung Stephanie PF04.09 Extracellular vesicles mediate innate immune activation in Hepatitis-Virus infections Jung Jik Han PF06.15 Isolation of blood-derived exosomes by dual size-exclusion chromatography

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 44 Abstract Directory Alpha Last Name First Name Poster # Poster Title Jung Youn Jae PS01.14 Stem Cell Exosomes as a Biochemical Cue for Recovery from Skin Photo-Aging Circulating extracellular vesicles released by blood flow restricted exercise have an altered Just Jesper PT08.05 miRNA profile and induce proliferation of skeletal muscle precursor cells Extracellular vesicles-mediated epithelial cell senescence by fibroblasts in IPF K Kadota Tsukasa PS01.02 pathogenesis Kalargyrou Aikaterini PS05.09 Investigating the mechanisms of molecular exchange in between retinal neurons Outer Membrane Vesicles of E. coli mediated resistance to ampicillin by carrying resistant Kameli Nader PF09.01 genes and proteins Data-driven identification of robust extracellular vesicle subpopulation in vitro models Kang Chi-Chih PF06.07 from patient blood Heterogeneity of cell-free fetal DNA in different types of placenta-derived extracellular Kang Matthew PT02.05 vesicles Precipitation based EV purification from rat plasma co-precipitates part of protein bound Karttunen Jenni PF06.04 miRNAs Protein Biomarker Discovery in Extracellular Vesicles isolated from Plasma for Colorectal Kasahara Keiko PT05.11 Cancer VEGFR2 shed from human umbilical vein endothelial cells on inside-out extracellular Kaur Sukhbir PF08.09 vesicles Kenyon Oliver IP 06 Fluorescence and 3D Light Scatter Activated Sorting of Small Particles GABARAPL1 is required for the secretion of pro-angiogenic extracellular vesicles during Keulers Tom PT04.10 hypoxia Human Liver stem cell derived EVs abrogates fibrotic markers in TGF-β1 activated Kholia Sharad PS01.10 Fibroblasts Urinary Exosomal and cell-free DNA Detects Somatic mutation and copy number alteration Kim Kwang Hyun PF01.10 in Urothelial Carcinoma of Bladder Hydrogel Microparticle based Multiplex qRT-PCR Profiling MicroRNAs in Urinary Kim Won Jin PF01.13 Extracellular Vesicle for Renal Transplantation Rejection Quantitative proteomics of extracellular vesicles derived from isogenic metastatic and Kim Kwang Pyo PS07.07 non-metastatic breast cancer in mice models Serum exosomal microRNAs as non-invasive biomarkers for human hepatocellular Kim Gyeonghwa PS08.12 carcinoma Kim Hyoseon PT03.04 Proteomic analysis of Acute Myeloid Leukemia-derived Extracellular vesicles Improving specificity and diagnostic power in extracellular vesicles - Easily incorporate Kirchner Benedikt PT03.12 isomiR analyses in existing pipelines Quantification and phenotyping of EVs by HPLC-size exclusion chromatography with on- Kitka Diana LBT01.06 line fluorescence detection Kleinjan Marije LBT01.14 Characterization of EVs isolated from differently processed bovine milk Procardiomyogenic and proangiogenic properties of extracellular vesicles derived from Kmiotek-Wasylewska Katarzyna PT06.11 genetically modified human induced pluripotent stem cells Characterization of Bovine Milk-Derived Extracellular Vesicles as Delivery System for Kogure Akiko PT07.13 Therapeutics Biologically counteractive extracellular vesicles are produced by neutrophilic granulocytes Kolonics Ferenc PF04.08 under different conditions Konte Tilen PF09.13 Isolation of fungal extracellular vesicles and their potential role in cell-cell communication Osteosarcoma derived extracellular vesicles mediated epigenetic alterations in Kornilov Roman PF03.03 mesenchymal stem cells Kotani Ai LBF05.02 HBV derived exosome is sysemically functional Muskelin regulates PrPC exosome packaging and membrane levels and influences prion Krasemann Susanne PF07.09 disease incubation time A path to ultra-low input microRNA sequencing from urinary extracellular vesicles after Ku Anson PS04.06 acoustic trap enrichment Immunomodulatory Function of Human Mesenchymal Stromal Cells-derived Extracellular Kui Lin PF04.01 Vesicles on Type-I Interferon Response in Human Plasmacytoid Dendritic Cells and Lupus Murine pDCs

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 45 Abstract Directory Alpha Last Name First Name Poster # Poster Title Kumazaki Minami PT04.07 Phenotypic Heterogeneity in Activated Fibroblasts Created by Extracellular Vesicles Identification of serum microRNAs as diagnostic and prognostic biomarkers for breast Kuo Wen-Hong LBT02.10 cancer Cancer-testis antigens MAGE-A proteins are incorporated into extracellular vesicles Kurg Reet PF02.03 released by cells. Monitoring the potential role of circulating miR-181b-5p in minimal residual disease in Kutszegi Nóra PS08.10 pediatric acute lymphoblastic leukemia A functional assessment of affinity isolated inflammatory extracellular vesicle Kuypers Sören PS04.16 subpopulations L La Salvia Sabrina PT08.13 Specific Blood EV Phenotype in Angiotensin induced HTN in Mice Microvesicles from T cells overexpress miR-146b-5p in HIV-1 infection and repress Lacroix Romaric PT08.09 endothelial activation Isolation and characterization of serum extracellular vesicles (EVs) from Atlantic salmon Lagos Leidy PF09.08 infected with Piscirickettsia salmonis Lang Kristina PS04.09 Isolation of clinical grade exosomes by a two-step FPLC purification method Lázaro-Ibáñez Elisa LBS08.09 Biodistribution, safety and toxicity profile of engineered extracellular vesicles Le Lay Soazig PS06.01 MIF adipocytokine uses extracellular vesicles as secretion pathway Extracellular vesicles as a drug delivery platform – post-production physico-chemical Le Saux Sarah PT07.07 modification and in vitro internalization Impact of pathogenic microbes and healthy microbiota by Lactobacillus-derived Lee Bao-Hong LBF04.07 extracellular vesicles Influence of storage condition on size and concentration of exosomes derived from canine Lee Seok Hee LBT01.05 oviduct cells and their uptake in cumulus cells in a time dependent manner Lee Seulbee PF03.06 Stem cell-derived exosomes as a biomaterial source for immune modulating therapy Characterization of Extracellular Vesicles using Raman Spectroscope for Label-free Cancer Lee Wooje PS08.03 Detection Exosome-type vesicular pool of phospholipases A2 in bronchoalveolar lavage fluid of Lekka Marilena PT09.10 patients with acute respiratory distress syndrome. A new role in the dissemination of inflammation? Endothelial colony-forming cell-derived exosomes attenuate pulmonary hypertension and Lesage Flore PS01.09 hypoplasia in neonatal rats Pancreatic islet-secreted exosomal microRNA-29 family members travel to liver and Li Jing LBS07.11 promote hepatic insulin resistance Extracellular Vesicles From Human iPS-Derived Cardiovascular Progenitor Cells Stimulate Lima Correa Bruna LBS07.04 the Proliferation of Cardiomyocytes in the Injured Heart Electrochemical and optical biosensing for the detection of cancer exosomes from breast Lima Moura Silio PS08.07 cancer cells A Novel Method for Identification of Extracellular Vesicles Derived from the Blood Brain Linden Jennifer PS05.04 Barrier and Their Role in Multiple Sclerosis Pathogenesis Linnemannstoens Karen PS07.01 Extracellular Vesicles in an in vivo system for macrophage migration Distinct anti-fibrotic effects of exosomes derived from normoxia and hypoxia cultured- Liu Wenhao PF08.11 endothelial colony forming cells Liu Dong PF08.13 Exosomes from adipose-derived stem cells induce angiogenesis CAF-derived exosomes remodel ECM by targeting lung fibroblasts via integrin α2β1 at the Liu Tingjiao PS07.09 pre-metastatic niche Scalable Xeno-Free Manufacturing of Extracellular Vesicles Derived from Human Lock Lye Theng PS04.20 Mesenchymal Stem Cells Omental Fat Extracellular Vesicles Promote Gastrointestinal Cancer Aggressiveness: A Loewenstein Shelly PS07.08 Potential Novel Mechanism of Peritoneal Metastasis The protein cargo of endothelial cell-derived extracellular vesicles released in response to Lombardo Giusy PT08.14 IL-3 identifies the Wnt signaling pathway as a relevant mediator of inflammation López de las Hazas María-Carmen LBS07.12 Walnuts supplementation alter exosomal miRNA in elderly subjects Lopez-Guerrero Jose Antonio PF01.06 Characterization of EXOsomes of the Spanish POpulation of REference (EXOSPORE)

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 46 Abstract Directory Alpha Last Name First Name Poster # Poster Title Mesenchymal stromal/stem cell-derived extracellular vesicles promote human cartilage Lorenowicz Magdalena PS01.04 regeneration Role of calcium signaling in the biogenesis of different types of extracellular vesicles Lőrincz Ákos LBS08.06 derived from the same cell Lovett Jason LBS07.01 Exercise-induced muscle damage, extracellular vesicles and microRNA Optimization of an explant culture model to characterize cancer-associated exosomes in Luu Anita PT04.02 canine osteosarcoma Detection and characterisation of apoptotic tumour cell-derived extracellular vesicles Lynch Catherine PS08.06 using Raman and surface enhanced Raman spectroscopy Nanoflow cytometry: quantitative and multiparameter analysis of single extracellular M Ma Ling IP 11 vesicles (40-150 nm) Differentiating C2C12 myocytes release exosomes and shedding microvesicles that trigger Maggio Serena PF04.21 different inflammatory responses in RAW264.7 macrophages Mamoun Robert PT07.02 Specific targeting of challenging membrane proteins on exosomes and their multiple uses Mannerström Bettina PT06.10 Non-coding landscape of extracellular vesicles RNA from stem cells Mantilla- Escalante Diana LBS08.02 Lipid-modulated exosomal miRNAs High yield mechanically-induced extracellular vesicles display as efficient regenerative Marangon Iris PT08.02 effect as their parental mesenchymal stem cells or starvation-induced EVs in vitro and in vivo in a model of chronic heart failure Maternal-Placental Messaging through extracellular vesicles is impacted by particulate air Mariani Jacopo PT02.11 pollution exposure In situ microarray-based detection of intravesicular proteins in exosome-like extracellular Martel Rosalie PT03.09 vesicles Systematic evaluation of techniques for the isolation and detection of small non-coding Martens-Uzunova Elena PS04.01 RNA from urine-derived extracellular vesicles Role of extracellular vesicles released by vascular endothelium on its own damage during Martínez Rojas Pedro LBF04.06 dengue virus infection The crosstalk between human osteosarcoma and endothelial cells in vitro through tumor- Mazumdar Alekhya PS07.10 derived extracellular vesicles An optimised workflow for the isolation and purification of extracellular vesicles from small McGee Margaret PF06.19 serum volumes Pancreatic cancer stem cell exosomes orchestrate an organized intra-tumor Melo Sonia PF02.19 communication network that contributes to tumor dynamics and plasticity Proteomic and metabolomic profiling of large microvesicles for their use as cancer Menck Kerstin PT03.03 biomarkers Exosomes and microvesicles miRNAs belong to the pro-inflammatory secretome of Mensa Emanuela LBF05.01 senescent endothelial cells TEM and Cryo-TEM microscopy as a tool to elucidate prokaryotic membrane vesicle Mercade Elena PS09.10 structure Extracellular vesicles from human dental pulp stem cells as proangiogenic strategy in Merckx Greet PF03.09 tooth regeneration Virtual Biorepository (VBR): a web-based service for sharing biofluid-, tissue-, cell- and Milosavljevic Aleksandar PS08.09 other bio-samples Miskei Judith PT04.14 Exosomes contain Wnt signals that regulate vascularization in lung cancer Validation of engineered cardiac grafts for the local delivery of multifunctional Monguió-Tortajada Marta PS01.01 extracellular vesicles for myocardial repair Identification of diagnostic biomarker on Circulating Extracellular Vesicles as a Novel Moon Pyong-Gon PT05.07 Biomarker for Colon Cancer Detection. Morales Olivier PT04.19 TEx induced tDC Osteoblast-Secreted Extracellular Vesicles Stimulate the Expansion Of CD34+ Human Morhayim Jess PF03.12 Umbilical Cord Blood Cells Increased venous and intra-atrial appendicular blood plasma levels of tissue factor- Mørk Morten PF08.06 exposing extracellular vesicles in atrial fibrillation patients Moulin Veronique PS03.11 New role of Alpha-2-macroglobulin into the shedding of microvesicles

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 47 Abstract Directory Alpha Last Name First Name Poster # Poster Title Intratracheal mesenchymal stem/stromal cells (MSCs)-derived extracellular vesicles (EVs) Muraca Maurizio PS01.11 significantly improve morphological and biochemical parameters in an animal model of Bronchopulmonary Dysplasia Urinary extracellular vesicles (uEVs) have unique characteristics as demonstrated by Musante Luca PF01.04 imaging and spectral cytometry N Nagy Corina LBF06.03 Neural-derived peripheral biomarkers for antidepressant response from plasma exosomes Najrana Tanbir PT06.03 Mechanical force induced EV-miRNAs play a role in fetal lung development Nam Sung-Wook PS02.03 Fabrication of an EV sorting and sensing device using nanostructures Exploration of extracellular vesicles from Ascaris suum provides evidence of parasite-host Nejsum Peter PT01.08 cross talk Tiotropium inhibits the release of proinflammatory extracellular vesicles by acetylcholine- Neri Tommaso PT09.12 stimulated lung epithelial cells ANXA6+-EVs from CAFs support pancreatic cancer aggressiveness through a PRT99- Nigri Jeremy PT04.09 dependent internalization process Njock Makon-Sébastien PF05.05 Sputum Exosomes: promising biomarkers for Idiopathic Pulmonary Fibrosis Optimization of a size exclusion chromatography protocol to isolate plasma-derived Nobre Rui PF06.09 extracellular vesicles for transcriptional biomarkers research Nolan John LBT03.07 Immunofluorescence Flow Cytometry of Extracellular Vesicle Surface Proteins Novaes Antonio PS06.07 Exosomes from high glucose-treated mesangial cells trigger dysfunction of podocytes Nunes-Xavier Caroline PF02.09 Oncogenic role of B7-H3 from tumor cell-derived extracellular vesicles. Determination of biological and technical variability at protein level in isolated urinary O Oeyen Eline PT03.13 extracellular vesicles of healthy individuals Integrating long and short sequencing data for a global overview of the endothelial O’Grady Tina PT03.11 extracellular vesicle RNA landscape Circulating miRNAs in plasma extracellular vesicles are potentials biomarkers in resistance Oliveira Luanda PF09.09 to HIV-1 infection Efficient isolation of extracellular vesicles from blood plasma based on iodixanol density Onódi Zsófia PF06.12 gradient ultracentrifugation combined with bind-elute chromatography Harnessing the Mesenchymal Stem Cells (hMSC) Secretome to Couple the RV/PA during Ortiz Luis PS01.06 Pulmonary fibrosis (PF) Nanoparticles, Liposomes and Exosomes as microRNA delivery systems for Osorio-Querejeta Iñaki PT07.09 Neurodegenerative disease; Remyelination inductors in Multiple Sclerosis. Osteikoetxea Xabier LBT03.04 Comparative lipidomics platform reveals distinct lipid profiles in extracellular vesicles Ozawa Patricia LBT02.03 Tumorigenic capacity of exosomes isolated from TNBC cells Differential fluorescence nanoparticle tracking analysis for enumeration of the P Pachler Karin PS09.06 extracellular vesicle content in mixed particulate solutions Paden Ljubisa PF01.11 Water intake depletes concentration of extracellular vesicles in peripheral blood Maria Elisabetta Palamà PS01.07 Exosomes derived from mesenchymal stem cells as a possible therapy for osteoarthritis Federica Analyzing leukemia derived extracellular vesicle modulation of immune activity in Pando Alejandro PF04.11 lymphocytes The effect of chemotherapy induced intercellular communication on breast cancer Pantazi Paschalia PS07.11 metastasis Snail modulates extracellular vesicles-mediated interleukin release by cells constituting Papiewska-Pajak Izabela PS07.06 pre-metastatic niche in human colorectal cancer Single cancer cell detection using microflow cytometry and ultrasound-mediated Paproski Robert PS08.04 extracellular vesicle release Enhancing accuracy of clinical predictions on shifted microflow cytometry data with signal Paproski Robert PS09.11 standardization Comparing extracellular vesicle enrichment methods for use on small sample volumes: Paris Bianca PF06.18 how low can we go? Anti-inflammatory activity of exosome-mimetic nanovesicles from mesenchymal stem Park Kyong-Su PT09.02 cells in septic mice

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 48 Abstract Directory Alpha Last Name First Name Poster # Poster Title Patil Neha PF04.13 Natural Killer (NK) cells and NK-exosomes in chronic bacterial lung infection Functional characterization of mucosal extracellular vesicles of rodents following Peck Bailey PS06.03 metabolic surgery Pereira Rinaldo PF04.03 LL-37 impacts extracellular microvesicles production by murine dendritic cells Systemic Lupus Erythematosus (SLE) and Rheumatoid Arthritis (RA) have increased Pereira Rinaldo PT09.09 concentration of serum microvesicles compared to healthy patients Biofilm related sRNA are differentially encapsulated in membrane vesicles from Perez-Cruz Carla PF09.02 Pseudomonas aeruginosa PAO1 Processing of the amyloid precursor protein in the exosomal pathway: propagation of Perez-Gonzalez Rocio PF07.02 Alzheimer’s disease pathology Plasma and urine-derived exosomes reveal a microRNA signature in hypertensive patients Perez-Hernandez Javier PF08.01 with albuminuria Persa Eszter PF06.14 Isolation of bone marrow extracellular vesicles for in vivo studies in mice Perut Francesca PT04.12 Exosomes secreted by human osteosarcoma cells promote angiogenesis Pietrowska Monika LBT03.02 Proteome of exosomes released by HPV(+) and HPV(-) head and neck cancer cells Pink Desmond PF01.02 Biomarker Development and Validation for Nanoscale Flow Cytometry of Liquid Biopsies Pink Desmond PF01.19 Designing nanoscale flow cytometry assays: Fixing, Blocking and Permeabilizing Samples Pink Desmond PF07.16 Characterization of the CSPα-Extracellular Vesicle Export Pathway Polakovicova Iva PF02.14 Role of microRNA-335-5p in gastric cancer derived extracellular vesicles E Polaschek Sandra PS03.05 Kinase modifiers of exosome secretion in PDAC cells Portnoy Victoria IP 03 A novel platform for a scalable, selective, and easy method to isolate extracellular vesicles Extracellular vesicles contained in malignant ascites contribute to progression of high Pospíchalová Vendula PS07.13 grade serous ovarian carcinoma MiRNA-146a-5p in Extracellular Vesicles Facilitate the Angiogenesis in High-Grade Prieto Vila Marta PT04.11 Bladder Cancer. R Radeghieri Annalisa PS01.15 Improving cell viability by extracellular vesicles from Amniotic fluid cells Multiple myeloma-derived exosomes carry EGFR ligand and are responsible for the Raimondo Stefania PT04.03 uncoupled bone remodeling A total transcriptome analysis of extracellular vesicles derived from the intestinal protozoa Ramirez Marcel PT01.05 Giardia intestinalis Rasmussen Rikke PS04.08 Antibody aggregates: A potential pitfall in the search of rare EV-populations MicroRNA-21 over expression in umbilical cord blood hematopoietic stem cells by Razmkhah Farnaz PF03.04 leukemia microvesicles Leukemia microvesicles induce LSC specific genes over expression in umbilical cord blood Razmkhah Farnaz PF03.07 hematopoietic stem cells Regev-Rudzki Neta LBF04.01 Malaria Parasite-Derived Vesicles Associate with the NF-kB Signaling Pathway Free flow electrophoresis allows preparation of extracellular vesicles fractions with high Reiter Christian LBT01.15 recovery and purity rates Extracellular vesicle isolation and characterization in the follow up of HCV-infected Revull Meritxell PF01.07 patients treated with directly-acting antiviral agents (DAAs) Rhim Taiyoun LBT02.08 Detection of lung cancer-specific membrane proteins in plasma exosomes Human mesenchymal stromal cell-derived exosomes promote wound healing in a mouse Ribault Alexandre LBS07.03 model of radiation-induced injury Characterization of subpopulations of circulating extracellular vesicles by imaging flow Ricklefs Franz PS08.05 cytometry Characterization of extracellular vesicles by transmission electron microscopy: comparison Rikkert Linda PS09.19 of negative staining protocols Ritz Simone PF04.10 Exploring the role of Extracellular Vesicles (EVs) in immune response Analyzing novel mechanisms involved in tumor-adipose tissue crosstalk during melanoma Robado de Lope Lucía PS07.02 metastasis: role of secreted exosomes and soluble factors Rodrigues Sílvia LBT01.04 Development of a standardized exosome production process for clinical use

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 49 Abstract Directory Alpha Last Name First Name Poster # Poster Title Morphology characteristics and miRNA of extracellular vesicles secreted during Rodríguez-Alvarez Lleretny PT02.06 blastulation discriminate competent bovine blastocysts Roig Carles David LBF06.07 Exosomes modulate brain endothelial cell function in neuroinflammation The effect of aspirin daily dose change on platelet-derived microvesicles in patients after Rosinska Justyna PF07.11 ischemic stroke Microvesicles Derived from Gene Modified “Mesenkillers”: Isolation, Characterization and Rossignoli Filippo PT07.04 Anti-Cancer Potential Plasmatic extracellular vesicles released in days during high particulate matter levels are Rota Federica PS06.06 able to activate endothelial cells Roy Sabrina LBF06.02 Plasma-based detection of gliomas Royo Félix PS03.06 Modifications of the glycome of extracellular vesicles affect their biodistribution in mice The extracellular RNA-Seq processing pipeline of the Extracellular RNA Communication Rozowsky Joel PT03.10 Consortium Russell Ashley PF07.14 Exosomes and neuroinflammatory microRNAs: cytokine-specific profiles Rutman Alissa PF04.15 Characterization of the immune potential of human islet extracellular vesicles Rzepiel Andrea PF05.13 Circulating microRNAs as potential biomarkers of pediatric acute lymphoblastic leukemia Neutral Sphingomyelinase 2 influences transfer of oligomeric alpha-synuclein through S Sackmann Valerie PF07.06 exosomal pathway and its relation to oxidative stress Saini Sharanjot PS07.16 Role of exosomes in inducing neuroendocrine differentiation in advanced prostate cancer Salas-Huenuleo Edison PS03.09 A simple method to label vesicles for visualization and in vivo tracking The effect of hypoxia on extracellular vesicles secretion from renal carcinoma and normal Samoylenko Anatoliy PF02.07 embryonic kidney cells Sanada Yohei PT06.02 Articular chondrocytes-derived EVs regulate osteoclastogenesis, but not osteogenesis Chloride intracellular channel protein 4 (CLIC4) is a serological cancer biomarker released Sanchez Vanesa PT05.16 from tumor epithelial cells via extracellular vesicles and required for metastasis. A comparative study of methodologies to encapsulate gold nanoparticles into exosomes Sancho María PS02.06 for theragnostics Atherosclerotic Patients have Lower Levels of BLTR1 Expressing Microvesicles Compared to Sanden Mathilde PT08.08 Healthy Individuals Sandor Gyongyver Orsolya LBS09.03 Analyzing factors resulting in the elevated EV release of pancreatic cancer cells Sanz-Rubio David PF05.09 Circulating Exosomal microRNAs in Obstructive Sleep Apnea Coronary artery bypass graft (CABG) surgery – Does the surgery procedure affect the EV Schlingschroeder Christina PT08.03 status? A protocol for rapid extraction of high quality RNA from urinary EVs used for the detection Schlumpberger Martin IP 04 of TMPRSS2:ERG fusion transcripts in prostate cancer subjects Pilot scale and GMP compliant production in STR bioreactor and purification process of Schmittgen Thomas LBT01.02 wild-type and engineered extracellular vesicles by TFF Schnatz Andrea LBF06.04 Delivery of ribosomes from glia to neurons From bench to bedside: A systematic approach to increased laboratory exosome Schuh Christina PS04.17 production Establishment of in vitro assays to monitor the immune-modulatory capacity of MSC- Schwich Esther PF03.02 derived EVs towards cytokine and T cell response for clinical application The association of total and vesicular blood HLA-G levels with disease stage and Schwich Esther PT04.15 circulating tumor cells in ovarian cancer patients Cell-line specific influence of breast cancer-derived extracellular vesicles on the Schwich Esther PT04.20 composition and immune response of peripheral cell subsets The combination of cell and gene therapy as tool to design a new generation therapy Sepúlveda Pilar PF03.05 based on MSC’s derived exosomes. Sepúlveda Pilar PT04.13 Unraveling Notch implication in exosome mediated angiogenesis of MDA 231 Sepúlveda Pilar PS03.10 Circulating exosomes as delivery mechanism of Free Fatty Acids (cFFA) Effects of extracellular vesicles deriving from BRAF inhibitor resistant melanoma cells on Shahaj Eriomina LBS09.07 immune cells: role of immune checkpoint expression

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 50 Abstract Directory Alpha Last Name First Name Poster # Poster Title Neutrophil-derived exosome drives the autoinflammatory responses of generalized Shao Shuai PT09.05 pustular psoriasis via activating NOD2 in keratinocytes Explosive versus penetrating mechanisms of combat injury in the generation of Sharrock Anna PF08.08 prothrombotic microvesicles Shatnyeva Olga PS02.05 Designer RNA binding proteins for loading exogenous RNA into extracellular vesicles A systematic review and meta-analysis of parameters affecting the the therapeutic Shekari Faezeh PT07.08 potential of mesenchymal stem cell-derived extracellular vesicles in preclinical studies Analysis of surface glycans on extracellular vesicles using lectin array and roles of their Shimoda Asako PS09.08 glycans in cellular recognition Development of Poreless Filter for Extracellular Vesicles Isolation and Staining for Prostate Shin Hyunwoo PF06.06 Cancer Diagnosis Extracellular vesicles secreted by dendritic cells can recruit mesenchymal stem/stromal Silva Andreia PS01.16 cells: in vitro and ex vivo evidence Novel label-free method for extracellular-vesicle enrichment from biological fluids and cell Singh Prateek PS04.14 culture medium Siqueira Ionara PS06.05 The aging process alters catalase activity in circulating extracellular vesicles of Wistar rats Siupa Agnieszka IP 10 Setting Benchmarks for Robust and Reproducible Nanoparticle Tracking Analyses Profiling Extracellular Vesicles Derived From Equine Mesenchymal Stem Cells And Tendon Sivelli Giulia LBS07.15 Derived Cells For Tendon Regeneration. Evaluation of usefulness of the mini-SEC method for purification of exosomes for mass Smolarz Mateusz LBT01.11 spectrometry proteomic studies Soares Martins Tânia LBT01.10 Comparative analyses of exosome isolation methods from distinct biofluids Urinary exosomes microRNAs: a future biomarkers in lupus patients with renal Solé Marcé Cristina LBF05.03 involvement. Son Soyoung LBS07.13 Mesenchymal stem cell-derived exosomes for treating liver fibrosis Extracellular vesicles released by tumor microenvironment and clonal monocytes induce a Sophie Park PF08.04 procoagulant climate within chronic myelomonocytic leukemia tumor niche. Extracellular vesicles from the parasitic nematode Trichuris muris: new insights into host- Sotillo Javier PT01.09 parasite communications Srinivasan Srimeenakshi PF01.15 Systematic study of exRNA isolation reveals presence of distinct exRNA carriers Maternal serum extracellular RNA as noninvasive biomarkers associated with abnormally Srinivasan Srimeenakshi PT02.08 invasive placenta Generation of engineered exosomes for targeted delivery of therapeutic microRNAs in CAP Strempel Nikola PT07.12 cells Plasma nanostructuring of the tools increases the yield of extracellular vesicles in blood Stukelj Roman PF06.16 isolates Membrane metalloproteinases regulation by insertion into microdomains and extracellular Suárez Montero Henar LBS09.05 vesicles Sui Zhigang PT03.15 New sample preparation method for exosome proteome analysis Identification of novel targets for colorectal cancer liquid biopsy by proteome-wide Sumazaki Makoto PT05.15 profiling of EVs from cultured viable tumor tissues Iron restriction is central to nutritional immunity, but does it affect the extracellular Swift Simon PF09.03 vesicles of bacterial pathogens? TNF-α and opsonized particles stimulate different type of extracellular vesicle production Szeifert Viktória PF04.20 from neutrophilic granulocyte Szvicsek Zsuzsanna PF02.12 Characterizing the extracellular vesicle (EV) production of colorectal cancer cell lines The effect of IFN-γ treatment on extracellular vesicles metabolite composition in breast T Tadokoro Hiroko PT04.17 cancer cells Takahashi Akiko PS08.14 The biology of exosome derived from senescent cells Investigation of monocyte-dependent activation of endothelial cells by microvesicles in Tan Ying Ying PT08.11 vitro Ha-RasV12 induced augmented secretion of Wnt5a and EpCAM containing exosome in Tang Ming Jer LBT02.02 MDCK cells

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 51 Abstract Directory Alpha Last Name First Name Poster # Poster Title Mesenchymal stromal cell-derived extracellular vesicles (EVs) as mediators of anti- Tasso Roberta PS01.03 inflammatory effects: endorsement of macrophage polarization Tey Sze Keong PS07.12 Role of Exosomes in Liver Cancer Metastasis Proteomic Identification of Exosome-Derived FAM3C as a Potential Biomarker for Non- Thuya Win Lwin PT05.03 small Cell Lung Cancer Quantitative analysis of nucleic acids in extracellular vesicles at the single-particle level via Tian Ye PS09.05 an ultrasensitive flow cytometer Different anti-inflammatory effects of extracellular vesicles from adipose-derived Tofiño-Vian Miguel PT09.11 mesenchymal stem cell or keratinocyte cell line on osteoarthritic cartilage Toribio VIctor PS03.07 A quantitative method to measure EV uptake. Trypanosoma cruzi induce macrophage expression of inflammatory factors and release of Torrecilhas Ana PT01.03 EVs that modulate infection by the parasite Exosomes increase SH-SY5Y neuroblastoma cells radioresistance by activating the AKT Tortolici Flavia PS08.18 survival pathway Toubarro Duarte LBS07.08 Vesicular release of Interleukin-36γ is Toll-like receptor dependent Differential contribution of extracellular vesicles from different settings to thrombin Tripisciano Carla PF08.05 generation Evaluation of the preanalytical conditions on the size, concentration and characteristics of Trøseid Anne Marie PF06.10 extracellular vesicles isolated from serum, EDTA- and citrated plasmas Extracellular vesicle analysis for biomarker identification in cerebral spinal fluid and blood Trupp Miles PF05.10 from patients with Parkinson’s Disease Different expression patterns of exosomal miRNAs under Cyclosporin A and Rapamycin Tubita Valeria PS07.19 treatment in distinct aggressiveness colorectal carcinomas. The role of Extracellular Vesicles -miRNAs of AML patients in the regulation of tumor Bone Tzoran Inna PT04.04 Marrow microenvironment U Ude Arinzechukwu LBS08.03 Role of Exosomes in Chemotherapy-induced Bystander Effect A proteome-wide catalog of viable renal cell carcinoma tissue-derived EVs, toward Ueda Koji PT03.01 development of cancer liquid biopsy diagnostics The role of extracellular vesicle-mediated miR-10a transfer in bone marrow Umezu Tomohiro PT04.01 microenvironment of patients with multiple myeloma Prostate cancer-derived extracellular vesicles facilitate osteoclast fusion and Urabe Fumihiko PS06.13 differentiation via enhancing filopodia formation in osteoclast precursors Isolation and characterization of human seminal plasma exosomes: vehicles involved in V Vago Riccardo PT02.10 spermatozoa motility properties and capacitation Exploring the neuroprotective function of extracellular vesicles containing small heat Van den Broek Bram PF07.15 shock proteins (HSPB1 and HSPB8) upon neuroinflammation Van Deun Jan PS04.07 EV-TRACK: evaluation, updates and future plans Unraveling the mechanism of action of milk-derived EV by linking their proteome to van Herwijnen Martijn PT03.14 relevant signaling pathways using an unbiased comprehensive bioinformatics approach The effect of Extracellular vesicles from Staphylococcus Aureus and Staphylococcus Vazirisani Forugh PF09.05 Epidermidis on RAW264.7 macrophages Vázquez Adrián LBT02.01 Role of exosomal Connexin43 in melanoma progression Plasma extracellular vesicles in patients with HIV and type 2 diabetes; a proteomic Vestad Beate LBT03.01 approach to search for HIV comorbidity biomarkers Vidal Lorena PF07.10 Study of retinal-extracellular vesicles in a model of retinitis pigmentosa: the rd10 mouse Villysson Annie PF09.06 Shiga toxin interactions with microvesicles Vinas Jose PS03.08 Ligand-receptor Interactions in exosome targeting in ischemic acute kidney injury Vogt Stefan LBS08.05 Unraveling the distribution of extracellular vesicles in vivo using recombinant tetraspanins W W Sodar Barbara LBT01.08 Low-density lipoprotein associates with extracellular vesicles via apolipoprotein B W Sodar Barbara PS03.02 Antibiotic-induced release of small extracellular vesicles with surface-associated DNA Wahlgren Jessica PF07.01 Extracellular vesicles as part of the search for Alzheimer´s disease blood based biomarkers Exosomes Derived from Hypoxic Glioma Cells Deliver miR-25 to Normoxic Cells to Elicit Wang Jiwei LBS09.04 Chemoresistance

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 52 Abstract Directory Alpha Last Name First Name Poster # Poster Title Exosomes derived from hypoxic GBM cells deliver miR-25 to normoxic cells to elicit Wang Jiwei PF02.06 chemoresistance Extracellular vesicles derived from aged mesenchymal stem cells improve the regeneration Wang Xiaoqin PF03.08 capacity of mesenchymal stem cells UVA induced plasma membrane damage promotes shedding of EVs from melanocytes and Wäster Petra PS08.17 activates cell proliferation Differential Interaction of Platelet-Derived Extracellular Vesicles with Leukocyte Subsets in Weber Viktoria PF04.17 Human Whole Blood Inflammatory molecules are cell-to-cell transported by Exosomes in the anti-inflammatory Weisshaar Maria PF04.14 human Autologous Conditioned Serum Welsh Joshua PF01.03 An accessible method of flow cytometer scatter standardization for EV analysis. Whitehead Meredith PS06.10 Role of vascular smooth muscle cell derived-exosomes in age-related vascular amyloidosis Von Willebrand factor and thrombospondin-1 in exosomes derived from blood outgrowth Wibowo Arief PF08.12 endothelial cells in ischemic heart disease. Wiklander Oscar PB LBT01.03 Engineered Extracellular Vesicles as Therapeutic Decoys Immunomagnetic enrichment of extracellular vesicles from mouse plasma improves Wild Stefan IP 12 sensitivity of analysis An in vivo Drosophila RNAi screen for identification of secretory multivesicular body Witte Leonie PS03.04 trafficking factors Metabolomic profiling of exosomes isolated from serum of head and neck cancer patients Włosowicz Agata LBS09.02 after radiotherapy – a preliminary study Isolation of intact extracellular vesicles (EVs) and comparison of EVs isolated from urine Woo Hyun-Kyung PS04.05 and plasma Identification the exosomes from adipose-derived stem and progenitor cells for hypoxic Wu Chia Ching LBF04.05 brain injury Isolation and Small RNA Sequencing of Follicular Fluid Exosomes from Single Ovarian Wyse Brandon PT02.12 Follicles Extracellular vesicles released by B-1 cells modulates macrophages response and alters X Xander Patricia PT01.02 the course of experimental Leishmania (Leishmania) amazonensis infection Lysosomal Inhibition in Triple-Negative Breast Cancer Cells Alters Exosome Composition Xu Jing PF02.17 and Function Induction of structural and functional effects of myeloma cells after Daratumumab Y Yakymiv Yuliya LBS09.08 treatment Yamayoshi Asako PS02.04 Novel antibody-mediated drug delivery system for targeting exosomal microRNA Yamazaki Shuji IP 07 Highly efficient and easy to use exosome isolation spin column with porous glass filter Yan Yan PS01.13 Myoblast-exosome is a mediator of protective signal of remote ischemic conditioning Isolation of extracellular vesicle-associated small RNA from canine mitral valve Yang Vicky LBT01.13 interstitial cells using ultracentrifugation and tangential flow filtration with size exclusion chromatography Yang Chun PT08.04 microRNAs enriched in lymphocytic microparticles exhibit antiangiogenic effect Comparing the efficiency of exosomes isolation from prostate and pancreatic cancer cell Yieng Chin Mei PS08.01 lines using size exclusion chromatography and ExoQuick-TC Discovery of an inhibitor for EV secretion in cancer cells using a small-molecule library Yoshioka Yusuke PT07.03 approach. In vitro and in vivo effects of plant ceramide to increase exosomes capable of eliminating Yuyama Kohei PF07.04 Alzheimer’s amyloid-ß Paving the way to the profiling of endometrial extracellular vesicles (EV)/exosomes as a Zarovni Natasa PT02.01 source of non-invasive biomarkers for guiding the successful embryo implantation Oncogenes systematically reprogram the exosome biogenesis pathway to promote Z Zhang Haifeng LBS09.06 tumorigenesis Stem Cell-Derived Extracellular Vesicles as a cell-free therapy for tissue repair/ Zhang Licheng PF03.10 regeneration: a systematic review and meta-analysis of Preclinical Studies Zhang Ying PF04.07 Cdc42 mediates CpG DNA-increased cellular uptake of extracellular vesicles Zhang Xiaogang PF06.11 Isolation of extracellular vesicles from human plasma using a novel three step protocol

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 53 Abstract Directory Alpha Last Name First Name Poster # Poster Title The contribution of chronic intermittent hypoxia to OSAHS: from the perspective of serum Zhang Huina PT03.06 extracellular microvesicle proteins Droplet microfluidics enabled single-exosome-counting immunoassays for cancer Zheng Lei PS08.08 diagnostics Aggregation-Induced Emission Probe/Graphene Oxide Aptasensor for Label-Free and Zheng Lei PS09.02 “Turn-On” Fluorescent Detection of Cancerous Exosomes Serum exosome concentration as a differential diagnosis marker for pulmonary Zheng Lei PT05.09 tuberculosis and non- small cell lung cancer Zhong Wenwan LBT01.09 Single vesicle counting enabled by DNA nanostructures Molecular Profiling Plasma Extracellular Vesicle Unveils Features Associated With Breast Zhong Zhenyu LBT02.07 Cancer Aggression, Metastasis And Invasion Co-isolation and analysis of extracellular vesicle (EV)-associated DNA and cell free DNA Zocco Davide PF01.14 (cfDNA) improves the diagnostic and prognostic value of circulating BRAF V600E in metastatic melanoma patients Biological and regenerative properties of extracellular vesicles from mesenchymal stem Zuba-Surma Ewa PF03.16 cells of various origin in cardiovascular regeneration. Zuppone Stefania PS02.01 Engineering exosomes as refined drug delivery vehicles

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 54 ISEV Sponsor Directory

AGC Asahi Glass Booth 29 FiberCell Systems Inc Booth 13 U1-5-1, Marunouchi 168 West Main Street #922 Chiyoda-ku, Tokyo 100-8405 New Market, Maryland 21774 AGC Asahi Glass provides innovative glass spin column for exosome isolation. (301) 471-1269 This kit enables easy-to-use and high capture yield. AGC Asahi Glass is a [email protected] world-leading glass solution provider and supplier. Suppliers of laboratory scale hollow fiber bioreactors for easy scale up of exosome production. HFBR allows the culture of cells at high density and under in vivo like cell culture conditions for concentrated exosome Apogee Flow Systems Ltd Booth 27 production without contamination from endogenous exosomes in serum. Unit 7 Grovelands Gram quantities of exosomes can be produced. Boundary Way Hemel Hempstead, England HP27TE +44 208 123 6831 FUJIFILM Wako Pure Chemical Corporation Booth 30 [email protected] 1-2, Doshomachi 3-Chome, Chuo-Ku Flow cytometers for small particle applications: Extracellular Vesicles, flow Osaka virometry, microbiology. +81 6 6203 3741 [email protected] FUJIFILM Wako Chemical Corporation is a world-renowned supplier of high- BD Biosciences Booth 3 purity laboratory chemicals for research and development based in Japan. Terre Bonne Park - A4 Our comprehensive product portfolio includes analytical reagents, fine Route de Crassier 17 chemicals and life science products. We focus on creating new value for our Eysins, 1262 Switzerland customers and are continuously engaged in developing new technologies. BD is one of the largest global medical technology companies in the world and is advancing the world of health by improving medical discovery, diagnostics HansaBioMed Life Sciences Booth 1 and the delivery of care. BD helps customers enhance outcomes, lower costs, increase efficiencies, improve safety and expand access to health care. Akadeemia Tee 15A, 12618 Tallinn (EE) (372) 656-1996 HansaBioMed Life-Sciences LLC is a leading Company entirely dedicated to Beckman Coulter Life Sciences Booth 2 research and development of products in the field of Extracellular Vesicle Torre Realia Plaza de Europa 41-43 Sciences. Unique HansaBioMed products are our Lyophlized Exosome 4ª Planta Standards and the ExoTEST kit, a double sandwich ELISA for exosome Barcelona, Catalonia 08908 quantification from human biofluids or cell media. Beckman Coulter Life Sciences is dedicated to improving the health of people around the world. The company’s global leadership and world-class ImmunoStep Booth 10 service and support delivers sophisticated instrument systems, reagents and services to life science researchers in academic and commercial laboratories, IMMUNOSTEP S.L. enabling new discoveries in biology-based research and development. Avda. Universidad de Coimbra, s/n VAT: B37373784 SALAMANCA, Castilla y Leon 37007 Exosome Plus Booth 25 +34923294827 [email protected] 10th floor, Annex, Catholic University of Korea St. Mary’s Hospital Seocho-gu, Banpo-Daero 222 IMMUNOSTEP is a Biotech company focused in the proteomic area that Seoul, Seoul-t’ukpyolsi 06591 research, develop, produce, market reagents & technologies both for research and diagnostics for Flow Cytometry. Our product areas include Exosome Plus is a biotech company striving to achieve greatness in EV- human & mouse cell immunophenotyping, apoptosis & diagnostic kits, related field in terms of isolation, diagnosis and quality control on vesicles cell-cycle analysis, and the higher method for the senstive detection of themselves.. We are currently releasing “Exo2P”, an EV isolation kit more exosomes by flow. suited for downstream analysis and application than any other contenders in the market.

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 55 ISEV Sponsor Directory

International Society Merck KGaA Booth 21 for Extracellular Vesicles (ISEV) Booth 19 Eschenstraße 5 19 Mantua Road Taufkirchen, Germany 82024 Mt. Royal, New Jersey 08011 49 61 5172 2000 +01 856-423-1621 [email protected] [email protected] Merck KGaA of Darmstadt, Germany is a leading company for innovative and ISEV is hosting its 2019 Annual Meeting in Kyoto, Japan. Stop by the booth, top-quality high-tech products in healthcare, life science and performance meet representatives from Kyoto and learn about the history, culture, and the materials. With extensive expertise in separations, reagents, biomarker city that awaits your visit! analysis, immunoassays, flow and imaging flow cytometry, Merck continues to provide advancements for basic, applied, and pharmaceutical research and manufacturing. Join our Flow Cytometry experts at our booth and find IZON Science Ltd Booth 31 out how our innovative range of technologies can maximize the results from The Oxford Science Park, Magdalen Centre your precious samples. Seeing more, accurately! 1 Robert Robinson Avenue Oxford, England OX4 4GA Miltenyi Biotec Booth 24 +44-1865-784-630 [email protected] Friedrich-Ebert-Strasse 68 Bergisch Gladbach, Nordrhein-Westfalen 51429 Izon Science is the world leading manufacturer of EV separation and 0049220483060 characterization tools. Its qEV systems using SEC columns have rapidly [email protected] become the EV separation method favored by experts. Izon’s TRPS measurement system is the only accurate, standardisable and practical Miltenyi Biotec provides products that advance biomedical research and method of measuring complex nano-bio particles, particularly EVs. cellular therapy. Our innovative tools support research from basic research to translational research to clinical application. Our more than 25 years of expertise includes immunology, stem cell biology, and cancer. Miltenyi Biotec Macrogen Booth 12 has more than 2,000 employees in 28 countries. 10F, 254 Beotkkot-ro, Geumcheon-gu World Meridian I NanoFCM INC. Booth 20 Seoul, 08511 Republic of Korea 82-2-2180-7000 Xiamen Pioneering Park [email protected] Chuangye Bldg 701 Xiamen, Fujian 361006 Macrogen is a global precision medicine company that predicts diseases 8618060971530 through big data integrated with genomic information. We provide services in [email protected] sequencing analysis (CES, NGS), microarray, oligo synthesis, GEM (Genetically Engineered Mouse) and bioinformatics. NanoFCM provides a versatile and powerful platform - Flow NanoAnalyzer for the multiparameter analysis of single functional nanoparticles down to 7nm, which has the capacity to measure the size, concentration, and biochemical Malvern Panalytical Booth 26 properties. To our knowledge, this is the only FCM that covers the entire size Malvern Instruments range of exosomes. 30 Rue Jean Rostand Orsay Cedex, Ile-de-France 91893 France NanoView Biosciences Booth 22 33 169 351 808 [email protected] 8 St Mary Boston, Massachusetts 02215 The detection and analysis of exosomes and microvesicles can be extremely +1.617.358.4833 challenging as many have a diameter of less than 100nm and are highly [email protected] heterogeneous. Malvern Panalytical’s unique nanoparticle tracking analysis (NTA), enables both size and concentration measurements simultaneously, NanoView Biosciences is developing tools and assays to assist in the whilst visual validation gives added confidence in the results obtained. translation of EV research into clinical diagnostics. NanoView’s technology provides single vesicle sizing, counting and phenotyping without the need for sample purification or fluorescent labeling. The technology is multiplexed, user variable independent and automated for increased sample throughput.

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 56 ISEV Sponsor Directory

Norgen Biotek Corp. Booth 17 Spectradyne LLC Booth 18 3430 Schmon Parkway 23875 Madison St, Suite A Thorold, Ontario L2V 4Y6 Torrance, California 90505 (905) 227-8848 +1 603-501-3295 [email protected] [email protected] Norgen Biotek provides researchers with innovative kits for Sample Spectradyne's mission is to improve the efficacy and safety of Collection/Preservation [cf-DNA/cf-RNA from Blood/Plasma/Serum, Urine, nanotechnology-related applications by analyzing nanoparticle mixtures Saliva], Molecular Diagnostics (MDx), and RNA/DNA/Protein Purification. Our with unprecedented resolution, and at speeds up to 10,000 particles/s. Our kits feature exceptional quality, ease-of-use and sensitivity. Norgen provides nCS1 instrument, using resistive pulse sensing, provides superior information researchers worldwide with the tools to address any sample preservation and about protein aggregates, synthetic nanoparticles, exosomes, and generally preparation challenge. into any nanoparticle mixture.

Oxford Nanoimaging Ltd Booth 16 Sysmex Corporation Booth 15 Jordan Hill Road 4-4-4 Takatsukadai, Nishi-ku Oxford, , England OX2 8DR Kobe, Hyogo 651-2271 +44 (0) 1865951820 +81-78-991-6040 [email protected] Sysmex provides a unique technology for precisely quantification of Exosome Oxford Nanoimaging has redesigned super-resolution instrumentation. We with easy sample handling in corporation with JVC KENWOOD. We offer novel removed the superfluous elements of traditional fluorescent microscopes digital counting to worldwide researchers in diagnostics, pharmaceutical and by doing so have created an instrument which is optimally designed to and basic science for further advanced medicine. Sysmex is a world leader in generate high-quality single-molecule data. The instrument supports various clinical laboratory systematization and solutions. modes of operation: single-molecule localization-based super-resolution for quantitative cellular imaging, TIRF, epifluorescence, and smFRET. System Biosciences (SBI) Booth 28 2438 Embarcadero Way Particle Metrix GmbH Booth 14 Palo Alto, California 94303 Am Latumer See 13 (650) 968-2200 Meerbusch, Nordrhein-Westfalen 40668 [email protected] +49 (0) 2150-70567-90 System Biosciences is a leading provider of innovative products and services [email protected] for Exosome Research. Our exosome portfolio, with our flagship ExoQuick line, Particle Metrix GmbH manufactures NTA and in-situ DLS Dynamic Light enables basic and translational researchers to better investigate exosomes Scattering analyzers for nanoparticles in body liquids. Both techniques are and discover disease biomarkers. We also offer exosome quantitation and complementary to each other, in size and concentration range. Applied to engineering tools, plus NGS, Mass Spec and NTA services. proteins, EVs, virus like particles, the analyzers deliver particle concentration, size, surface charge and immuno-phenotype information.

Rosetta Exosome Inc. Booth 11 67 Seobinggo-ro, Yongsan-gu 103-1502, Park Tower Apt. Seoul, Seoul-t'ukpyolsi 140-762 Republic of Korea 82-2-6101-2228 Founded in 2016, Rosetta Exosome Inc. has developed integrated next- generation platform technologies for the development of innovative exosome-based diagnostics and therapeutics against intractable diseases. At ISEV 2018, Rosetta Exosome Inc. is launching ExoLutE®-a better solution for extracellular vesicle isolation from cell culture conditioned medium and biological fluids.

Annual Meeting — ISEV2018 | Barcelona, Spain | 2–6 May 2018 57