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Sponsored By 17th Annual Undergraduate Research Symposium in the Chemical and Biological Sciences The College of Natural and Mathematical Sciences; The Department of Chemistry and Biochemistry & The Department of Biological Sciences Sponsored by: National Institute of General Medical Sciences of the National Institutes of Health (NIGMS/NIH) th 17 Annual Undergraduate Research Symposium in the Chemical and Biological Sciences Table of Contents SCHEDULE OF EVENTS ................................................................................................................................. 3 MORNING POSTER SESSION AFTERNOON POSTER SESSION Biological Sciences Biological Sciences (Posters 1 - 48) (Posters 125 - 164) Group A ..................................................................... 4 Group S .................................................................. 146 Group B ................................................................... 12 Group T ................................................................. 154 Group C ................................................................... 20 Group U ................................................................. 162 Group D ................................................................... 28 Group V ................................................................. 170 Group E ................................................................... 36 Group W ................................................................ 177 Group F .................................................................... 44 Group X ................................................................. 184 Group G ................................................................... 51 Chemical Sciences Chemical Sciences (Posters 49 - 83) (Posters 165 - 205) Group H ................................................................... 59 Group Y ................................................................. 192 Group I .................................................................... 67 Group Z ................................................................. 200 Group J .................................................................... 75 Group AA .............................................................. 208 Group K ................................................................... 83 Group BB............................................................... 216 Group L ................................................................... 91 Group CC............................................................... 223 Group DD .............................................................. 231 Biochemical & Molecular Biology Biochemical & Molecular Biology (Posters 84 - 124) (Posters 206 - 245) Group M .................................................................. 99 Group EE ............................................................... 239 Group N ................................................................ 106 Group FF ............................................................... 247 Group O ................................................................ 114 Group GG .............................................................. 254 Group P ................................................................. 122 Group HH .............................................................. 261 Group Q ................................................................. 130 Group II ................................................................. 269 Group R ................................................................. 138 Group JJ ................................................................. 277 2 th 17 Annual Undergraduate Research Symposium in the Chemical and Biological Sciences Schedule of Events Time Event 8:00 am SYMPOSIUM CHECK-IN & ON-SITE REGISTRATION Lobby, University Center, 3rd Floor 8:00 am – LIGHT CONTINENTAL BREAKFAST 9:00 am UC312, University Center, 3rd Floor 9:00 am – OPENING REMARKS & WELCOME ADDRESS 9:30 am Dr. Freeman Hrabowski, President, University of Maryland, Baltimore County (UMBC) Dr. William R. LaCourse, Dean, College of Natural & Mathematical Sciences, UMBC ENG027, Engineering Bldg., (Lecture Hall 5) 9:45 am – MORNING POSTER SESSION 11:45 pm A – G: Biological Sciences H – L : Chemical Sciences M - R: Biochemistry and Molecular Biology Ballroom, University Center, 3rd Floor 10:30 am – WORKSHOPS 11:30 am Professional Communication: A very, very short introduction Making the Right Impression to ethics for scientists Susan Hindle, Career Services, UMBC Dr. Simon Stacey, Director, The Honors College UC310, University Center, 3rd Floor CASTLE, UC115, University Center, 1st Floor 11:45 pm – BUFFET LUNCH 12:35 pm (gratis for registered guests with symposium name badge) The Commons – Main Street 12:45 pm – AFTERNOON POSTER SESSION 2:45 pm S – X: Biological Sciences Y – DD : Chemical Sciences EE – JJ: Biochemistry and Molecular Biology Ballroom, University Center, 3rd Floor 1:30 pm – WORKSHOPS 2:30 pm Repeat of workshop titles and locations above 3:00 pm – PLENARY TALK 4:00 pm “Transition states, Hilde Mangold, and you” Dr. David Asai, Howard Hughes Medical institute ENG027, Engineering Bldg., (Lecture Hall 5) 4:00 pm AWARDS PRESENTATION ENG027, Engineering Bldg., (Lecture Hall 5) 3 th 17 Annual Undergraduate Research Symposium in the Chemical and Biological Sciences Morning Poster Session Group A - Biological Sciences Poster Title, Author(s) & Affiliation(s) # 1. OVEREXPRESSION OF FRUCTOSE BIS-PHOSPHATE ALDOLASE IN CHLAMYDOMONAS Abravi Rose Gbemafu1, Rudolph Park2, Amrita Madabushi1, and Stephen M. Miller2 1Baltimore City Community College, Life Science Institute, 801 West Baltimore Street, Baltimore, MD 21201 2Department of Biological Sciences, UMBC, 1000 Hilltop Circle, Baltimore, MD 21250 2. ANTI-HIV THERAPY Deirdre Johnson and Ihid Leao Department of Natural Sciences, UMES, Student Services Center Lane, Princess Anne, MD 21853 3. C-PEPTIDE SECRETIONS AND DIFFUSION FROM RAT INSULINOMA CELLS USING A 3-D PRINTED FLUIDIC PLATE DEVICE Azel King1, Chengpeng Chen2, Yueli Liu2, and Dana Spence2 1Department of Biology, Medgar Evers College, 1638 Bedford Avenue, Brooklyn, NY 11225 2Department of Chemistry, Michigan State University, 578 Shaw Lane, East Lansing, MI 48824 4. OVEREXPRESSION OF CAH1 IN CHLAMYDOMONAS REINHARDTII Nicholas Often1, Rudolph Park2, Amrita Madabushi1, and Stephen M. Miller2 1Baltimore City Community College, 2901 Liberty Heights Avenue, Baltimore, MD 21215 2Department of Biological Sciences, UMBC, 1000 Hilltop Circle, Baltimore, MD 21250 5. BLOOD SENSORS: DEVELOPMENT OF BIOSENSORS FOR THE MEASUREMENT OF FACTOR XA AND THROMBIN CONCENTRATIONS IN BLOOD Ashley N. Stewart and Peter B. Berget Department of Biological Sciences, University of the Sciences, 600 S 43rd Street, Philadelphia, PA 19106 6. DESIGNING AN ELECTROSPUN SCAFFOLD FOR TREATMENT OF DIABETICS’ WOUNDS Juliet Strauss, Jennifer R. Weiser, and W. Mark Saltzman Department of Biomedical Engineering, Yale University, 55 Prospect Street, New Haven, CT 06511 7. TESTING AFFINITY OF COMPUTATIONALLY-DESIGNED HUMANIZED ANTIBODIES TO A TUMOR ANTIGEN Hannah C. Wastyk1, Margie E. Ackerman, Ph.D. 2,3,4, Yoonjoo Choi5, Chris Bailey-Kellogg5, Ming-Ru Wu3,4, Albert Gacerez3,4, Charles Sentman3,4, and Casey K. Hua2 1Department of Chemistry and Biochemistry, University of Delaware, 102 Brown Laboratory, Newark, DE 19716 2Department of Cellular and Molecular Biology, Dartmouth College, 7560 Remsen Building, Hanover, NH, 03755 3Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755 4Department of Microbiology and Immunology, Geisel School of Medicine, 1 Rope Ferry Rd, Hanover, NH 03755 5Department of Computer Science, Dartmouth College, 6211 Sudikoff Lab, Hanover, NH 03755 4 th 17 Annual Undergraduate Research Symposium in the Chemical and Biological Sciences OVEREXPRESSION OF FRUCTOSE BIS-PHOSPHATE ALDOLASE IN CHLAMYDOMONAS Abravi Rose Gbemafu1, Rudolph Park2, Amrita Madabushi1, and Stephen M. Miller2 1Baltimore City Community College, Life Science Institute, 801 West Baltimore Street, Baltimore, MD 21201 2Department of Biological Sciences, UMBC, 1000 Hilltop Circle, Baltimore, MD 21250 Algae hold great promises for production of liquid fuels but current yields are not high enough to compete with fossil fuels. Therefore there is a need to engineer algae to accumulate more biomass more quickly. Chlamydomonas reinhardtii is the E. coli of algae—many molecular genetic tools have been developed to manipulate it and its genome is sequenced and well characterized. In this study we are focusing on the Calvin cycle, a photosynthetic pathway localized to the stroma of the chloroplast that fixes carbon dioxide and produces sugar. The enzyme fructose bis-phosphate aldolase (FBA) is believed to carry out a key control step in the Calvin cycle. FBA combines glyceraldehyde-3- phosphate and dihydroxy acetone phosphate to form fructose-1,6-bisphosphate, a precursor to the 5-carbon sugar ribulose bisphosphate (RuBP) that must be regenerated in each round of the Calvin cycle. The main aim of our study is to determine whether we can increase the growth rate of C. reinhardtii by increasing the expression of FBA. To that end we have gene synthesized the coding sequence for C. reinhardtii FBA using C. reinhardtii chloroplast codon bias and are subcloning this sequence into our chloroplast transformation vector. Progress toward this goal will be reported. After transforming and selecting for spectinomycin
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