Profiling and Characterization of Embryo and Seminal

Total Page:16

File Type:pdf, Size:1020Kb

Profiling and Characterization of Embryo and Seminal Downloaded from orbit.dtu.dk on: Sep 28, 2021 Profiling and characterization of embryo and seminal fluid extracellular vesicle- associated microRNAs in Sus scrofa: a possible role in differentiation and developmental communication processes Nawaz, Muhammad; Højbøge, Tina Rødgaard; Maugeri, Marco; Mehryar, Matin; Villemoes, Klaus; Heegaard, Peter M. H.; Callesen, Henrik; Valadi, Hadi Published in: Journal of Extracellular Vesicles Publication date: 2016 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Nawaz, M., Højbøge, T. R., Maugeri, M., Mehryar, M., Villemoes, K., Heegaard, P. M. H., Callesen, H., & Valadi, H. (2016). Profiling and characterization of embryo and seminal fluid extracellular vesicle-associated microRNAs in Sus scrofa: a possible role in differentiation and developmental communication processes. Journal of Extracellular Vesicles, 5, 169-169. [PF7.16]. General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. The Fifth International Meeting of ISEV ISEV2016 Rotterdam, The Netherlands, 4 – 7 May, 2016 Abstracts ISEV2016 is organized by The Local Organizing Committee Chair Edit I Buzás (Hungary), Aled Clayton (United Kingdom), Dolores Di Vizio (USA), Juan Manuel Falcon-Perez (Spain), Guido Jenster (The Netherlands), Lorraine O’Driscoll (Ireland), Yong Song Gho (South Korea), Marjolein van Driel (The Netherlands), Hans van Leeuwen (The Netherlands), Guillaume van Niel (France), Marca HM Wauben (The Netherlands), Kenneth W Witwer (USA), María Yáñez-Mó (Spain) Together with the Executive ISEV Board (2014 – 2016) President: Jan Lötvall Secretary General: Clotilde Théry Interim Treasurer: Kenneth W Witwer Executive Chair Science / Meetings: Marca Wauben Executive Chair Education: Yong Song Gho Executive Chair Communication: Andrew Hill Members at Large: Peter Quesenberry, Kenneth W Witwer, Susmita Sahoo, Dolores Di Vizio, Chris Gardiner, Edit I Buzás, Hidetoshi Tahara, Suresh Mathivanan, Igor Kurochkin Diamond Sponsor Gold Sponsor Plus Silver Sponsors Bronze Sponsors Qiagen Additional Sponsor Wednesday, 4 May 2016 8:00 – 9:00 a.m. Registration 8:30 – 9:00 a.m. Welcome Coffee 9:00 – 9:20 a.m. Opening Sessions 9:20 – 10:30 a.m. Plenary Session 1 – EVs in Cancer Speakers: K. Pantel (Germany) and D. Lyden (USA) Room: Willem Burger 10:30 – 11:00 a.m. Networking Coffee 11:00 – 12:30 p.m. Symposium Session 1 Symposium Session 2 Symposium Session 3 EVs and Implications to EV Proteomics and Cell Biology and EVs I Cancer Therapy Lipidomics Room: Jurriaanse Room: Willem Burger Room: van Weelde 12:30 – 1:30 p.m. Lunch 1:30 – 2:15 p.m. Oral with Poster Session 1 Oral with Poster Session 2 Oral with Poster Session 3 EV Isolation, Characterization and EVs, Immunity and More EVs in Cancer and Other Stem Cells Room: van Weelde Diseases Room: Willem Burger Room: Jurriaanse 2:15 – 3:00 p.m. Symposium Session 4 Symposium Session 5 Symposium Session 6 EVs of the CNS EV Isolation EVs in Cancer Transcriptomics Room: van Weelde Room: Jurriaanse Room: Willem Burger 3:00 – 3:30 p.m. Networking Coffee 3:30 – 5:00 p.m. Biotech Sponsored session: Symposium Session 8 Biotech Sponsored session IZON CARIS Life Sciences EVs and Tumor Environment I (3:30–4:00 p.m.) (3:30–3:45 p.m.) Room: Willem Burger Symposium Session 7 Symposium Session 9 (3:45–5:00 p.m.) (4:00–5:00 p.m.) EVs in Metabolic Syndrome, Fatty EV Biomarkers for Cancer I Liver Disease and Cancer Room: Jurriaanse 5:00 – 8:00 p.m. Poster Session 1 - Cell Biology of EVs: Biogenesis and Transfer Poster Session 2 - EVs in Tumor Metastasis Poster Session 3 - Analysis of EVs in Body Fluids Poster Session 4 - EVs as Drug Delivery System for Antitumoral Therapies & Vaccination Poster Session 5 - EVs in Acute and Chronic Inflammation Poster Session 6 - EV Proteomics & Lipidomics Poster Session 7 - EV Isolation Poster Session 8 - EVs in Prostate Cancer 6:30 – 8:30 p.m. Satellite Event Meet the National and International Societies Room: Jurriaanse Thursday, 5 May 2016 8:00 – 8:45 a.m. Meet the Experts Session 1 Meet the Experts Session 2 Meet the Experts Session 3 EV Heterogeneity: Can EVs in Regenerative Medicine EVs in Infectious Disease we Overcome the Room: Jurriaanse Room: van Weelde Confusion? Room: Willem Burger 9:00 – 10:15 a.m. Symposium Session 10 Symposium Session 11 Symposium Session 12 EVs in Cross-species EV Characterization: Flow EVs in Cardiovascular Disease Communication Cytometry Room: van Weelde Room: Jurriaanse Room: Willem Burger 10:15 – 10:45 a.m. Networking Coffee 10:45 – 12:30 p.m. Plenary Session 2 - Exosomes and viruses Speakers: R. C. Gallo (USA), L. Margolis (USA), Shilpa Buch (USA) Room: Willem Burger 12:30 – 1:30 p.m. Lunch 1:30 – 3:00 p.m. Symposium Session 13 Symposium Session 14 Symposium Session 15 EVs in Remodelling and Engineering EVs EVs and Tumor Environment II Repair Room: Jurriaanse Room: Willem Burger Room: van Weelde 3:00 – 3:30 p.m. Networking Coffee 3:30 – 4:30 p.m. Symposium Session 16 Symposium Session 17 Symposium Session 18 New Techniques for EV EV Biomarkers for Cancer II EVs in Neuropathies Characterization Room: Jurriaanse Room: Willem Burger Room: van Weelde 5:00 – 8:00 p.m. Poster Session 1 - EVs, Microbes & Cross-species Communication Poster Session 2 - EVs in Cardiovascular Disease Poster Session 3 - EVs in Cardiovascular Disease and Coagulation Poster Session 4 - EVs in Stem Cells Poster Session 5 - EVs as Cancer Biomarkers Poster Session 6 - EVs in the CNS Poster Session 7 - EV Characterization from Different Sources or Different Subtypes Poster Session 8 - Late Breaking Poster Session 1 6:30 – 8:30 p.m. Satellite Event ISEV-ISAC-ISTH Workshop Room: Jurriaanse Friday, 6 May 2016 8:00 – 8:45 a.m. Meet the Experts Session 4 Meet the Experts Session 5 Meet the Experts Session 6 EVs as Modulators of Drug EV-Associated Versus Extracellular RNAs: EVs or not Resistance and Tumor Soluble Functional Molecules EVs Metastasis Room: Jurriaanse Room: van Weelde Room: Willem Burger 9:00 – 10:15 a.m. Symposium Session 19 Symposium Session 20 Symposium Session 21 EVs in the Immune System EVs in Cancer Metastasis I EVs in Reproduction and Room: Jurriaanse Room: Willem Burger Pregnancy Room: van Weelde 10:15 – 10:45 a.m. Networking Coffee 10:45 – 11:25 a.m. Plenary Session 3 – EVs in Cell Biology Speaker: F. Sanchez-Madrid (Spain) Room: Willem Burger 11:25 – 12:30 p.m. ISEV General Assembly 12:30 – 1:30 p.m. Lunch JEV Editorial Board Meeting 1:30 – 2:45 p.m. Symposium Session 22 Symposium Session 23 Symposium Session 24 EVs in Acute and Chronic EVs in Cancer Metastasis II EVs in Immune Modulation in Inflammatory Disease Room: Jurriaanse Bacterial and Parasitic Infections Room: van Weelde Room: Willem Burger 2:45 – 3:15 p.m. Networking Coffee 3:15 – 4:15 p.m. Diamond Sponsor Session Caris Life Sciences Room: Willem Burger 4:15 – 5:00 p.m. Symposium Session 25 Symposium Session 26 Symposium Session 27 EVs Viruses and Viral EVs in Cellular Differentiation and Cell Biology of EVs II Infections Development Room: Willem Burger Room: Jurriaanse Room: van Weelde 5:00 – 7:30 p.m. Poster Session 1 - Non-cancer Biomarkers Poster Session 2 - EVs and Cancer Poster Session 3 - EVs and the Immune System Poster Session 4 - EVs in the Tumor Microenvironment Poster Session 5 - MSCs & Tissue Regeneration and Morphogenesis Poster Session 6 - Novel Technical Developments in EV Characterization Poster Session 7 - Late Breaking Posters Session 2 7:30 p.m. Networking Event (registration required) Location: Laurens Church Saturday, 7 May 2016 8:00 – 8:45 a.m. Experts Meet Session 1 Experts Meet Session 2 Experts Meet Session 3 Biofluids: Blood Biofluids: Milk Biofluids: Urine 9:00 – 10:00 a.m. Late Breaking Oral Session 1 Late Breaking Oral Session 2 Late Breaking Oral Session 3 Room: Willem Burger Room: van Weelde Room: Ruys, van Ruckevorsel, Plate Zaals 10:00 – 10:30 a.m. Networking Coffee 10:30 – 11:50 a.m. Plenary Session 4 with Featured Abstracts ISEV2016 Room: Willem Burger 11:50 – 12:35 p.m. Special Achievement Award, Wrap-up Sessions, Oral and Poster Awards 12:35 – 12:45 p.m. Closing Remarks and Announcement of ISEV2017 Please note that the order and/or list of affiliations for some authors may be incorrect for the abstracts in this document. This is due to incorrect processing of the submitted data by the software used for abstract submission. Despite our efforts to manually correct as many entries as possible, and following several requests to authors for assistance, we were not able to correct the submissions with 100% accuracy. We sincerely apologize for the inconvenience to the authors and will do our utmost to ensure this problem does not recur in the future. The ISEV2016 organizers. Scientific Program ISEV 2016 meeting ABSTRACTS Wednesday May 4, 2016 Room: Willem Burger Plenary Session 1 - EVs in cancer 9:20-10:30 a.m. Chairs: Clotilde The´ry and Pascale Zimmermann Speakers: Klaus Pantel (University Medical Center Hamburg-Eppendorf, Germany) Liquid Biopsy in Cancer Patients David C.
Recommended publications
  • Attenuation of Pathogenic Immune Responses During Infection with Human and Simian Immunodeficiency Virus (HIV/SIV) by the Tetracycline Derivative Minocycline
    Attenuation of Pathogenic Immune Responses during Infection with Human and Simian Immunodeficiency Virus (HIV/SIV) by the Tetracycline Derivative Minocycline Julia L. Drewes1, Gregory L. Szeto1¤, Elizabeth L. Engle1, Zhaohao Liao1, Gene M. Shearer2,M. Christine Zink1*, David R. Graham1,3* 1 Department of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, 2 Experimental Immunology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America, 3 Johns Hopkins Bayview Proteomics Center, Department of Medicine, Division of Cardiology, Johns Hopkins School of Medicine Bayview Campus, Baltimore, Maryland, United States of America Abstract HIV immune pathogenesis is postulated to involve two major mechanisms: 1) chronic innate immune responses that drive T cell activation and apoptosis and 2) induction of immune regulators that suppress T cell function and proliferation. Both arms are elevated chronically in lymphoid tissues of non-natural hosts, which ultimately develop AIDS. However, these mechanisms are not elevated chronically in natural hosts of SIV infection that avert immune pathogenesis despite similarly high viral loads. In this study we investigated whether minocycline could modulate these pathogenic antiviral responses in non-natural hosts of HIV and SIV. We found that minocycline attenuated in vitro induction of type I interferon (IFN) and the IFN-stimulated genes indoleamine 2,3-dioxygenase (IDO1) and TNF-related apoptosis inducing ligand (TRAIL) in human plasmacytoid dendritic cells and PBMCs exposed to aldrithiol-2 inactivated HIV or infectious influenza virus. Activation- induced TRAIL and expression of cytotoxic T-lymphocyte antigen 4 (CTLA-4) in isolated CD4+ T cells were also reduced by minocycline.
    [Show full text]
  • University of Groningen Mechanics of Extracellular Vesicles
    University of Groningen Mechanics of extracellular vesicles derived from malaria parasiteinfected Red Blood Cells Sorkin, Raya; Vorselen, Daan; Ofir-Birin, Yifat; Roos, Wouter H.; MacKintosh, Fred C.; Regev-Rudzki, Neta; Wuite, Gijs J. L. Published in: Journal of Extracellular Vesicles DOI: 10.3402/jev.v5.31552 IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below. Publication date: 2016 Link to publication in University of Groningen/UMCG research database Citation for published version (APA): Sorkin, R., Vorselen, D., Ofir-Birin, Y., Roos, W. H., MacKintosh, F. C., Regev-Rudzki, N., & Wuite, G. J. L. (2016). Mechanics of extracellular vesicles derived from malaria parasiteinfected Red Blood Cells. Journal of Extracellular Vesicles, 5, 86. https://doi.org/10.3402/jev.v5.31552 Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Download date: 12-11-2019
    [Show full text]
  • Human Perivascular Stem Cell-Derived Extracellular Vesicles Mediate Bone
    RESEARCH ARTICLE Human perivascular stem cell-derived extracellular vesicles mediate bone repair Jiajia Xu1, Yiyun Wang1, Ching-Yun Hsu1, Yongxing Gao1, Carolyn Ann Meyers1, Leslie Chang1, Leititia Zhang1,2, Kristen Broderick3, Catherine Ding4, Bruno Peault4,5,6, Kenneth Witwer7,8, Aaron Watkins James1* 1Department of Pathology, Johns Hopkins University, Baltimore, United States; 2Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang, China; 3Department of Surgery, Johns Hopkins University, Baltimore, United States; 4Department of Orthopaedic Surgery, Orthopaedic Hospital Research Center, UCLA, Orthopaedic Hospital, Los Angeles, United States; 5Centre For Cardiovascular Science, University of Edinburgh, Edinburgh, United Kingdom; 6MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, United Kingdom; 7Department of Molecular and Comparative Pathobiology, Johns Hopkins University, Baltimore, United States; 8Department of Neurology, Johns Hopkins University, Baltimore, United States Abstract The vascular wall is a source of progenitor cells that are able to induce skeletal repair, primarily by paracrine mechanisms. Here, the paracrine role of extracellular vesicles (EVs) in bone healing was investigated. First, purified human perivascular stem cells (PSCs) were observed to induce mitogenic, pro-migratory, and pro-osteogenic effects on osteoprogenitor cells while in non- contact co-culture via elaboration of EVs. PSC-derived EVs shared mitogenic, pro-migratory, and pro-osteogenic properties of their parent cell. PSC-EV effects were dependent on surface- associated tetraspanins, as demonstrated by EV trypsinization, or neutralizing antibodies for CD9 or CD81. Moreover, shRNA knockdown in recipient cells demonstrated requirement for the CD9/ CD81 binding partners IGSF8 and PTGFRN for EV bioactivity. Finally, PSC-EVs stimulated bone repair, and did so via stimulation of skeletal cell proliferation, migration, and osteodifferentiation.
    [Show full text]
  • Announcing the ISEV2020 Special Achievement Award Recipients
    Thomas Jefferson University Jefferson Digital Commons Kimmel Cancer Center Faculty Papers Kimmel Cancer Center 10-1-2020 Announcing the ISEV2020 special achievement award recipients: Andrew Hill and Edit Buzás; and the recipient of the ISEV2020 special education award: Carolina Soekmadji. Kenneth W Witwer Lucia R Languino Alissa M Weaver Marca H Wauben Follow this and additional works at: https://jdc.jefferson.edu/kimmelccfp Part of the Oncology Commons Let us know how access to this document benefits ouy This Article is brought to you for free and open access by the Jefferson Digital Commons. The Jefferson Digital Commons is a service of Thomas Jefferson University's Center for Teaching and Learning (CTL). The Commons is a showcase for Jefferson books and journals, peer-reviewed scholarly publications, unique historical collections from the University archives, and teaching tools. The Jefferson Digital Commons allows researchers and interested readers anywhere in the world to learn about and keep up to date with Jefferson scholarship. This article has been accepted for inclusion in Kimmel Cancer Center Faculty Papers by an authorized administrator of the Jefferson Digital Commons. For more information, please contact: [email protected]. Received: 30 July 2020 Accepted: 8 September 2020 DOI: 10.1002/jev2.12021 EDITORIAL Announcing the ISEV special achievement award recipients: AndrewHillandEditBuzás;andtherecipientoftheISEV special education award: Carolina Soekmadji The International Society for Extracellular Vesicles (ISEV) is pleased to announce two Special Achievement Awards that were presented at the virtual ISEV2020 annual meeting, 20–22 July 2020. ISEV Special Achievement Awards are given each year to one or more individuals who have made outstanding contributions to the field of extracellular vesicle (EV) research or have per- formed extraordinary service to ISEV (Witwer, Hill, & Tahara, 2019).
    [Show full text]
  • Isct2021 Program
    MAY 26–28 ISCT2021 VIRTUAL Orchestrating Global CGT Translation Building Consensus for the Path Forward PROGRAM MAY 26–28 ISCT2021 VIRTUAL WELCOME FROM THE ISCT PRESIDENT Dear Friends and Colleagues, a day, 3 concurrent tracks), and all of which will be available for viewing on demand for the remainder of 2021. Extra effort We ARE friends AND colleagues! Just as ISCT is a Society has been invested for enhanced virtual Networking, with a full like no other, ISCT 2021 Virtual New Orleans will continue ISCT 2021 Networking Day on May 25. During the Networking our breakthrough last year in providing a virtual platform Day, you can attend the inaugural Investigators to Investors with industry leading presentation technology that is im- (I to I) Workshop, organized by the ISCT Business Models & mersive and informative, enlightening and engaging in a Investment Commercialization Sub-Committee, which will multi-dimensional platform that reflects our Society values. bring together cell and gene therapy investors for a half-day We promote innovation in translational research. We strive educational event providing unparalleled access to cell and for excellence in everything we do. We respect and support gene therapy key opinion leaders. diversity and inclusivity. We uphold the highest ethical stan- dards. We serve our members and advocate for our Society. The anchor for your ISCT program is the six plenary ses- sions. Leading off, our President Elect Jacques Galipeau Over the past year, the world has seen great tragedy and will chair the plenary “COVID-19: Understanding the Path scientific advances to meet pandemic challenges. The field Forward for Mesenchymal Stromal Cell Therapies.” For the of cell and gene therapy continues to break new ground, Presidential Plenary, representing the Commercialization advancing medical care for patients worldwide.
    [Show full text]
  • Extracellular Vesicles and Nanoerythrosomes: the Hidden Pearls of Blood Products
    Academic Dissertations from The Finnish Red Cross Blood Service number 64 SAMI VALKONEN | Extracellular Vesicles and Nanoerythrosomes: The Hidden Pearls of Blood Products Blood Nanoerythrosomes: Pearls of Extracellular and Hidden The | Vesicles SAMI VALKONEN SAMI VALKONEN Extracellular Vesicles and Nanoerythrosomes: The Hidden Pearls of Blood Products ISBN 978-952-5457-48-3 (print) ISBN 978-952-5457-49-0 (pdf) ISSN 1236-0341 FRC BS: 64 http://ethesis.helsinki.fi Turku 2019 Painosalama Oy ACADEMIC DISSERTATION, NUMBER 64 Doctoral School of Health Sciences Doctoral Programme in Biomedicine Molecular and Integrative Biosciences Research Programme Faculty of Biological and Environmental Sciences University of Helsinki and Finnish Red Cross Blood Service EXTRACELLULAR VESICLES AND NANOERYTHROSOMES: THE HIDDEN PEARLS OF BLOOD PRODUCTS Sami Valkonen ACADEMIC DISSERTATION To be presented for public examination, with the permission of the Faculty of Biological and Environmental Sciences of the University of Helsinki, in Nevanlinna Auditorium of the Finnish Red Cross Blood Service, Kivihaantie 7, Helsinki, on 19th December 2019, at 12 noon. Helsinki 2019 ACADEMIC DISSERTATIONS FROM THE FINNISH RED CROSS BLOOD SERVICE, NUMBER 64 Supervisors Adjunct Professor Pia Siljander Faculty of Biological and Environmental Sciences University of Helsinki Doctor Saara Laitinen Department of Research & Development Finnish Red Cross Blood Service Thesis Committee Associate Professor Susanna Fagerholm Faculty of Biological and Environmental Sciences University of
    [Show full text]
  • Downloaded From
    Canonical Type I IFN Signaling in Simian Immunodeficiency Virus-Infected Macrophages Is Disrupted by Astrocyte-Secreted CCL2 This information is current as of September 27, 2021. Luna Alammar Zaritsky, Lucio Gama and Janice E. Clements J Immunol published online 9 March 2012 http://www.jimmunol.org/content/early/2012/03/09/jimmun ol.1103024 Downloaded from Why The JI? Submit online. http://www.jimmunol.org/ • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average by guest on September 27, 2021 Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2012 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. Published March 9, 2012, doi:10.4049/jimmunol.1103024 The Journal of Immunology Canonical Type I IFN Signaling in Simian Immunodeficiency Virus-Infected Macrophages Is Disrupted by Astrocyte-Secreted CCL2 Luna Alammar Zaritsky,* Lucio Gama,* and Janice E. Clements*,† HIV-associated neurologic disorders are a mounting problem despite the advent of highly active antiretroviral therapy. To address mechanisms of HIV-associated neurologic disorders, we used an SIV pigtailed macaque model to study innate immune responses in brain that suppress viral replication during acute infection.
    [Show full text]
  • NCUR Proceedings
    NCUR 2021 Proceedings A Diffusion Approach for Plasma Synthesis of Superhard Tantalum Borides Physics/Astronomy - Time: Tue 3:30pm-4:30pm - Session Number: 745 Aaditya Rau, Dr. Aaron Catledge, Department of Physics, The University of Alabama at Birmingham, 1720 2nd Ave S, Birmingham, Alabama 35294 Aaditya Rau Tantalum borides are a set of refractory metal compounds that have become of interest due to their desirable mechanical, thermal, and electrical properties. While these compounds have been previously synthesized in a variety of methods, key issues related to sample size, sample integrity, and implementation feasibility limit their use. In this study, a microwave plasma chemical vapor deposition (MPCVD) reactor was used to diffuse boron into tantalum substrates using a feedgas mixture of hydrogen and diborane. Specifically, the role of substrate temperature and substrate bias in influencing the chemical composition and mechanical properties of the borided tantalum was investigated. X-ray diffraction shows that high substrate temperature is favorable for TaB2 formation, with samples made at great than 775°C having a mean surface hardness of 40 GPa along with increased strain in the tantalum body-centered cubic lattice. Once the strained tantalum becomes locally supersaturated with boron, TaB and TaB2 precipitate. The combination of precipitation hardening as well as solid solution hardening may help explain the measured superhardness, as measured by nanoindentation. Application of a negative bias voltage to the substrate during the CVD process was not found to further increase the hardness, and in some cases decreased the measured hardness significantly, possibly due to etching effects from increased ion bombardment. These results show that MPCVD is a viable method for synthesis of superhard borides based on plasma-assisted diffusion.
    [Show full text]
  • Pharmacokinetics and Biodistribution of Extracellular Vesicles Administered Intravenously and Intranasally to Macaca Nemestrina
    bioRxiv preprint doi: https://doi.org/10.1101/2021.07.28.454192; this version posted July 29, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Pharmacokinetics and biodistribution of extracellular vesicles administered intravenously and intranasally to Macaca nemestrina Authors Tom Driedonks1, Linglei Jiang1, Bess Carlson 1, Zheng Han2,3, Guanshu Liu2,3, Suzanne E. Queen1, Erin N. Shirk1, Olesia Gololobova1, Lyle Nyberg1, Gabriela Lima1, Kayla Schonvisky1, Natalie Castell1, Mitchel Stover, Selena Guerrero-Martin1,4, Riley Richardson1, Barbara Smith5, Charles P. Lai6, Jessica M. Izzi1, Eric K. Hutchinson1, Kelly A.M. Pate1,4, Kenneth W. Witwer1,7,8,* 1. Dept. Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, US 2. Russell H. Morgan Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, MD, US 3. F.M. Kirby Research Center, Kennedy Krieger Institute, Baltimore, MD, US 4. Division of ComParative Medicine and DePartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, US 5. DePt. Cell Biology, Johns HoPkins University School of Medicine, Baltimore, MD, US 6. Institute of Atomic and Molecular Sciences, Academia Sinica, TaiPei, Taiwan 7. DePt. of Neurology, Johns HoPkins University School of Medicine, Baltimore, MD, US 8. The Richman Family Precision Medicine Center of Excellence in Alzheimer’s Disease, Johns Hopkins University School of Medicine, Baltimore, MD, US *CorresPondence: [email protected] 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.07.28.454192; this version posted July 29, 2021.
    [Show full text]
  • Highlights of the São Paulo ISEV Workshop on Extracellular Vesicles in Cross-Kingdom Communication
    Journal of Extracellular Vesicles ISSN: (Print) 2001-3078 (Online) Journal homepage: http://www.tandfonline.com/loi/zjev20 Highlights of the São Paulo ISEV workshop on extracellular vesicles in cross-kingdom communication Rodrigo P. Soares, Patrícia Xander, Adriana Oliveira Costa, Antonio Marcilla, Armando Menezes-Neto, Hernando Del Portillo, Kenneth Witwer, Marca Wauben, Esther Nolte-`T Hoen, Martin Olivier, Miriã Ferreira Criado, Luis Lamberti P. da Silva, Munira Muhammad Abdel Baqui, Sergio Schenkman, Walter Colli, Maria Julia Manso Alves, Karen Spadari Ferreira, Rosana Puccia, Peter Nejsum, Kristian Riesbeck, Allan Stensballe, Eline Palm Hansen, Lorena Martin Jaular, Reidun Øvstebø, Laura de la Canal, Paolo Bergese, Vera Pereira-Chioccola, Michael W. Pfaffl, Joëlle Fritz, Yong Song Gho & Ana Claudia Torrecilhas To cite this article: Rodrigo P. Soares, Patrícia Xander, Adriana Oliveira Costa, Antonio Marcilla, Armando Menezes-Neto, Hernando Del Portillo, Kenneth Witwer, Marca Wauben, Esther Nolte- `T Hoen, Martin Olivier, Miriã Ferreira Criado, Luis Lamberti P. da Silva, Munira Muhammad Abdel Baqui, Sergio Schenkman, Walter Colli, Maria Julia Manso Alves, Karen Spadari Ferreira, Rosana Puccia, Peter Nejsum, Kristian Riesbeck, Allan Stensballe, Eline Palm Hansen, Lorena Martin Jaular, Reidun Øvstebø, Laura de la Canal, Paolo Bergese, Vera Pereira-Chioccola, Michael W. Pfaffl, Joëlle Fritz, Yong Song Gho & Ana Claudia Torrecilhas (2017) Highlights of the São Paulo ISEV workshop on extracellular vesicles in cross-kingdom communication, Journal of Extracellular Vesicles, 6:1, 1407213, DOI: 10.1080/20013078.2017.1407213 To link to this article: https://doi.org/10.1080/20013078.2017.1407213 © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
    [Show full text]
  • Summary of the ISEV Workshop on Extracellular Vesicles As Disease Biomarkers, Held in Birmingham, UK, During December 2017
    Journal of Extracellular Vesicles ISSN: (Print) 2001-3078 (Online) Journal homepage: https://www.tandfonline.com/loi/zjev20 Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017 Aled Clayton, Dominik Buschmann, J. Brian Byrd, David R. F. Carter, Lesley Cheng, Carolyn Compton, George Daaboul, Andrew Devitt, Juan Manuel Falcon-Perez, Chris Gardiner, Dakota Gustafson, Paul Harrison, Clemens Helmbrecht, An Hendrix, Andrew Hill, Andrew Hoffman, Jennifer C. Jones, Raghu Kalluri, Ji Yoon Kang, Benedikt Kirchner, Cecilia Lässer, Charlotte Lawson, Metka Lenassi, Carina Levin, Alicia Llorente, Elena S. Martens- Uzunova, Andreas Möller, Luca Musante, Takahiro Ochiya, Ryan C Pink, Hidetoshi Tahara, Marca H. M. Wauben, Jason P. Webber, Joshua A. Welsh, Kenneth W. Witwer, Hang Yin & Rienk Nieuwland To cite this article: Aled Clayton, Dominik Buschmann, J. Brian Byrd, David R. F. Carter, Lesley Cheng, Carolyn Compton, George Daaboul, Andrew Devitt, Juan Manuel Falcon-Perez, Chris Gardiner, Dakota Gustafson, Paul Harrison, Clemens Helmbrecht, An Hendrix, Andrew Hill, Andrew Hoffman, Jennifer C. Jones, Raghu Kalluri, Ji Yoon Kang, Benedikt Kirchner, Cecilia Lässer, Charlotte Lawson, Metka Lenassi, Carina Levin, Alicia Llorente, Elena S. Martens-Uzunova, Andreas Möller, Luca Musante, Takahiro Ochiya, Ryan C Pink, Hidetoshi Tahara, Marca H. M. Wauben, Jason P. Webber, Joshua A. Welsh, Kenneth W. Witwer, Hang Yin & Rienk Nieuwland (2018) Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017, Journal of Extracellular Vesicles, 7:1, 1473707, DOI: 10.1080/20013078.2018.1473707 To link to this article: https://doi.org/10.1080/20013078.2018.1473707 © 2018 The Author(s).
    [Show full text]
  • Summary of the ISEV Workshop on Extracellular Vesicles As Disease Biomarkers, Held in Birmingham, UK, During December 2017
    Journal of Extracellular Vesicles ISSN: (Print) 2001-3078 (Online) Journal homepage: http://www.tandfonline.com/loi/zjev20 Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017 Aled Clayton, Dominik Buschmann, J. Brian Byrd, David R. F. Carter, Lesley Cheng, Carolyn Compton, George Daaboul, Andrew Devitt, Juan Manuel Falcon-Perez, Chris Gardiner, Dakota Gustafson, Paul Harrison, Clemens Helmbrecht, An Hendrix, Andrew Hill, Andrew Hoffman, Jennifer C. Jones, Raghu Kalluri, Ji Yoon Kang, Benedikt Kirchner, Cecilia Lässer, Charlotte Lawson, Metka Lenassi, Carina Levin, Alicia Llorente, Elena S. Martens- Uzunova, Andreas Möller, Luca Musante, Takahiro Ochiya, Ryan C Pink, Hidetoshi Tahara, Marca H. M. Wauben, Jason P. Webber, Joshua A. Welsh, Kenneth W. Witwer, Hang Yin & Rienk Nieuwland To cite this article: Aled Clayton, Dominik Buschmann, J. Brian Byrd, David R. F. Carter, Lesley Cheng, Carolyn Compton, George Daaboul, Andrew Devitt, Juan Manuel Falcon-Perez, Chris Gardiner, Dakota Gustafson, Paul Harrison, Clemens Helmbrecht, An Hendrix, Andrew Hill, Andrew Hoffman, Jennifer C. Jones, Raghu Kalluri, Ji Yoon Kang, Benedikt Kirchner, Cecilia Lässer, Charlotte Lawson, Metka Lenassi, Carina Levin, Alicia Llorente, Elena S. Martens-Uzunova, Andreas Möller, Luca Musante, Takahiro Ochiya, Ryan C Pink, Hidetoshi Tahara, Marca H. M. Wauben, Jason P. Webber, Joshua A. Welsh, Kenneth W. Witwer, Hang Yin & Rienk Nieuwland (2018) Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017, Journal of Extracellular Vesicles, 7:1, 1473707, DOI: 10.1080/20013078.2018.1473707 To link to this article: https://doi.org/10.1080/20013078.2018.1473707 © 2018 The Author(s).
    [Show full text]