PA2G4 Antibody (F54781)

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PA2G4 Antibody / EBP1 / ErbB3-binding protein 1 (F54781) Catalog No. Formulation Size F54781-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml F54781-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml Bulk quote request Availability 1-3 business days Species Reactivity Human Format Purified Clonality Polyclonal (rabbit origin) Isotype Rabbit Ig Purity Purified UniProt Q9UQ80 Localization Cytoplasmic, nuclear Applications Immunofluorescence : 1:25 Flow cytometry : 1:25 (1x10e6 cells) Immunohistochemistry (FFPE) : 1:25 Western blot : 1:500-1:1000 Limitations This PA2G4 antibody is available for research use only. IHC testing of FFPE human lung carcinoma tissue with PA2G4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining. Immunofluorescent staining of fixed and permeabilized human U-251 cells with PA2G4 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red). Western blot testing of human 1) A431 and 2) Jurkat cell lysate with PA2G4 antibody. Predicted molecular weight ~44 kDa. Flow cytometry testing of human A2058 cells with PA2G4 antibody; Blue=isotype control, Green= PA2G4 antibody. Description PA2G4 is an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. The protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. It is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. It has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Application Notes The stated application concentrations are suggested starting points. Titration of the PA2G4 antibody may be required due to differences in protocols and secondary/substrate sensitivity. Immunogen A portion of amino acids 228-255 from the human protein was used as the immunogen for the PA2G4 antibody. Storage Aliquot the PA2G4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles. Ordering: Phone:858.663.9055 | Fax:1.267.821.0800 | Email:[email protected] Copyright © NSJ Bioreagents. All rights reserved.

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EBP1/PA2G4 Rabbit Pab
Leader in Biomolecular Solutions for Life Science EBP1/PA2G4 Rabbit pAb Catalog No.: A5376 Basic Information Background Catalog No. This gene encodes an RNA-binding protein that is involved in growth regulation. This A5376 protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA Observed MW processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor 44kDa and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle Calculated MW regulatory genes through its interactions with histone deacetylases. This protein has 38kDa/43kDa been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been Category identified. Primary antibody Applications WB, IHC Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 5036 Q9UQ80 IHC 1:50 - 1:200 Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-394 of human EBP1/PA2G4 (NP_006182.2). Synonyms PA2G4;EBP1;HG4-1;p38-2G4 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using EBP1/PA2G4 antibody (A5376) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
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A Dissertation Entitled the Androgen Receptor
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10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 PA2G4 Antibody (Center R243) Blocking Peptide Synthetic peptide Catalog # BP2848d Specification PA2G4 Antibody (Center R243) Blocking PA2G4 Antibody (Center R243) Blocking Peptide - Peptide - Background Product Information PA2G4 is an RNA-binding protein that is Primary Accession Q9UQ80 involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome PA2G4 Antibody (Center R243) Blocking Peptide - Additional Information assembly and the regulation of intermediate and late steps of rRNA processing. The protein can interact with the cytoplasmic domain of Gene ID 5036 the ErbB3 receptor and may contribute to transducing growth regulatory signals. It is also Other Names a transcriptional co-repressor of androgen Proliferation-associated protein 2G4, Cell receptor-regulated genes and other cell cycle cycle protein p38-2G4 homolog, hG4-1, regulatory genes through its interactions with ErbB3-binding protein 1, PA2G4, EBP1 histone deacetylases. It has been implicated in Target/Specificity growth inhibition and the induction of The synthetic peptide sequence used to differentiation of human cancer cells. generate the antibody <a href=/products/AP2848d>AP2848d</a> PA2G4 Antibody (Center R243) Blocking was selected from the Center region of Peptide - References human PA2G4. A 10 to 100 fold molar excess to antibody is recommended. Zhang,Y., Mol. Cancer Ther. 7 (10), 3176-3186 Precise conditions should be optimized for a (2008)Zhang,Y., Cancer Lett. 265 (2), 298-306 particular assay. (2008)Okada,M., J. Biol. Chem. 282 (50), 36744-36754 (2007) Format Peptides are lyophilized in a solid powder format.
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bioRxiv preprint doi: https://doi.org/10.1101/088815; this version posted December 6, 2016. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Genome-Wide Association Study Reveals First Locus for Anorexia Nervosa and Metabolic Correlations Authors Duncan, E.L.* ... 210 additional authors Thornton, L.M. Hinney, A. Daly, M.J. Sullivan, P.F. Zeggini, E. Breen, G. Bulik, C.M.* * Corresponding authors Abstract Anorexia nervosa (AN) is a serious eating disorder characterized by restriction of energy intake relative to requirements, resulting in abnormally low body weight. It has a lifetime prevalence of approximately 1%, disproportionately affects females1,2, and has no well replicated evidence of effective pharmacological or psychological treatments despite high morbidity and mortality2. Twin studies support a genetic basis for the observed aggregation of AN in families3, with heritability estimates of 48%-74%4. Although initial genome-wide association studies (GWASs) were underpowered5,6, evidence suggested that signals for AN would be detected with increased power5. We present a GWAS of 3,495 AN cases and 10,982 controls with one genome-wide significant locus (index variant rs4622308, p=4.3x10-9) in a region (chr12:56,372,585- % 56,482,185) which includes six genes. The SNP-chip heritability (h"#$) of AN from these data is 0.20 (SE=0.02), suggesting that a substantial fraction of the twin-based heritability stems from common genetic variation.
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