REPORT TWO SPECIES of GENUS: COLEPS (C. HIRTUS and C. ELONGATUS) FOUND in RESERVOIR at DHANEGOAN, OSMANABAD (MS) Pawar S

Available Online at http://www.recentscientific.com International Journal of Recent Scientific International Journal of Recent Scientific Research Research Vol. 4, Issue, 3, pp.202 -207, March, 2013 ISSN: 0976-3031 REPORT TWO SPECIES OF GENUS: COLEPS (C. HIRTUS AND C. ELONGATUS) FOUND IN RESERVOIR AT DHANEGOAN, OSMANABAD (MS) Pawar S. B and Shembekar V. S Department of Zoology and Fishery Science, Rajashri Shahu Mahavidyalaya, Latur (M.S.)

ARTICLE INFO ABSTRACT

Article History: Coleps is free-living ciliated protozoan. It is primarily a member of the Prostomatea Received 16thJanuary, 2013 (Schewiakoff, 1896). In the present investigation, author has identified and re-described Received in revised form 22th, January, 2013 two species of genus Coleps, C. hirtus and C. elongatus. It is reported from reservoir at Accepted 15th February, 2013 Dhanegoan, Osmanabad (MS). Published online 28th March, 2013

INTRODUCTION varies from species to species. There are 2- 8 spinous projection are present at or near the posterior end of the body. The member of Class Prostomatea (Schewiakoff, 1896) of Some of the species possess one or more long caudal cilia at phylum Ciliophora, are more or less ovoid with cilia in rows. the posterior end. Present author found these two species of This class includes Subclass Holotricha (Stein) in these this genus from freshwater reservoir. individuals uniform Ciliation over the body surface, nutrition C. hirtus (O.F. Muller, 1786). is holozoic or saprozoic. These holotricous ciliates are widely C. elongatus (Ehrenberg, 1838). and abundantly distributed in all fresh and salt waters. This subclass includes Order Gymnostomatida or Prorodontida Description of the species: (Corliss, 1974). In the individuals of this subclass cytostome C. hirtus (O. F. Muller, 1786) opens directly to the outside, cytopharynx with trichites. Body The Coleps hirtus was first reported by O.F.Muller 1786. The ciliature is simple, no oral cilia. This order has the Family name Coleps hirtus given by Nitzsch (1827) and Ehrenberg Colepidae (Ehrenberg, 1833) in which cytostome at anterior (1830, 1831). Coleps hirtus also describe by stein(1867), end. Body barrel shaped with pellicular plates, uniform fromental (1874), Kent(1880-1882), Mupas(1885,1888,1889), ciliation. Genus Coleps is a member of this Family. Bustschli (1887-1889), Schewiakoff(1893,1896), Roux MATERIAL AND METHOD (1901), Bhatia( 1916), Gosh (1921), Gulati(1925), Noland (1925,1936), Lepsi(1926), Sandon (1927), Khal (1935), Bick The water samples were collected from Reservoir at (1972, 1974), Mahajan & Nair (1974), foissner et al., (1994), Dhanegoan, Osmanabad (MS). The observations on ciliates Kasai (2001), Kiyose (2001,2003) who represented same were done after their movements were slowed down with genus. Shaikh (2006) reporte the C. hirtus. methyl cellulose. For fixation Schaudinn’s fluid was used and permanent preparation was made by Dry sliver impregnation The body of Coleps hirtus is barrel shaped and covered in (Klein, 1928, 1958) and tungsto phosphoric haematoxylene regularly arranged prominent ectoplasmic plates. These plates method. are composed of amorphous calcium carbonate. There are about 20 longitudinal rows of pellicular plates. It is a common RESULT AND DISCUSSION holotricha ciliate in fresh water habitats, especially in situation where algae and other plant material has begun decompose. Description of the Genus The body of the Coleps hirtus is anterior as well as posterior is The Genus Coleps was first reported by Nitzsch (1827) and rounded or slightly flattened which is tooth like projection of they are widely and abundantly distributed in all fresh and salt the plates. Body is measures about 70-110µm in length and water. Body form of this genus is constant and barrel shaped 40-63µm in width. The oral aperture is circular and apically with regularly arranged ectoplasmic plates. Cytostome at situated and it is surrounded by special plates. They are anterior end of the body and it is directly open to the outside. scavengers and the mouth, which is at the forward end of a It is surrounded by slightly longer cilia. Often spinous basket like pellicle of calcareous plates or inconspicuous. projection at or near posterior end of the body; one or more There are two main groups of plates with four meridional rows long caudal cilia ( Kudo, 1966). Pellicular plates are present, of windows each. Near about 14 to 18 longitudinal rows of which are regularly arranged in longitudinal rows and number plates. There are three spinous processes at the posterior end

* Corresponding author: Pawar S. B E-mail address: [email protected] International Journal of Recent Scientific Research, Vol. 4, Issue, 3, pp.225- 226, March, 2013 of the body. Ciliation is uniform over the whole body i.e. in References regular longitudinal kineties along the striation in the plates. S.N. Bhamare, S. V. Nikam, B. N. Jadhav (2012): Prevalence The movement of Coleps hirtus is circular and it swims and morphological details in the common freshwater rapidly. They occur in all type of water containing organic detritus. It feed up on decaying matter and in the bottom and ciliate, Coleps hirtus from Nasik district of Maharashtra. Elixir Aquaculture 45(2012) 7787-7792. surface water also. Shaikh T.T.(2006): Studies on some free-living and parasitic Description of the species ciliates Ph.D Thesis of Dr. B. A. M. Univ. Aurangabad. C. elongatous (Ehrenberg, 1838) Deshmukh N. Z. (2010): Studies on some aquatic and soil protozoa from Aurangabad Vicinity Ph. D. Thesis of Dr. The individual of this species have barrel shaped body. Which B. M.A.Univ. Aurangabad. is covered by regularly arranged prominent ectoplasmic Corliss J.O. (1959): The ciliate protozoa: Characterization pellicular plates which are made up of amorphous calcium Classification and Guide to the Literature. Oxford, New carbonate? Body is elongated and measured about 14.60µ to York etc; Pergamon Pree. 455pages. 21.5µ in width and 36.87µ to 42.99µ in length. Deshmukh Finlay B.J. Clark K.J. Cowling A.J et al (1988): On the (2010) reported this species. Body is measured about 15.89µ abundances and distribution of protozoa and their food in to 24.97µ in width and 38.59µ to 45.4µ in length. C. elongatus a productivity freshwater pond. Eur. J. protisol.23: 205- has 14-17 rows of plates. Noland reported 13 rows. 217. Anterior end of the body is somewhat flattened while posterior Bary B.M. (1950): Four new speies of fresh water ciliates end is rounded. Oral aperture is circular and surrounded by from new zealond. Zool. Publ. Victoria. Univ. Coll: 2:1- slightly long cilia. At the posterior end there are three spinous 19 (1950b) speces are observed. Body has uniform ciliation along regular Borror A. C. (1972): Revision of the order hypotrichida longitudinal rows of plates. The ciliate moves along the axis (ciliophora, protozoa). J. protozol. 19:1-23. with a moderate speed and also swims in a circular path. They Noland (1925): A review of the genus Coleps with description are commonly found in all type of water containing organic of two new species Trans Am Microsc. Soc; 44(1): 3-13. detritus. It also occurs in reservoir, lakes and ponds. They are Klein B. M. (1958): The dry sliver method and its proper use. feed on saprophytically upon other Protozoans and on algae, J. Protozol. 5:99. th flagellates and small ciliates or rotifers. Kudo R.R (1966): Protozoology 5 Ed. Charles C. Thomas Springfield, Illinios; 1174. Acknowledgement Kirby (1950): Material and methods in the study of Protozoa. Univ. of Calif. Pree. Authors are thankful to head of the Department of Zoology & Fishery Science,Rajashri Shahu Mahavidyalaya, Latur (M.S.)

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