Supplemental Table 1: that show altered expression in HepG2 cells in the presence of exogenously added let-7

Gene Title Symbol RefSeq Transcriptp- IDvalue(TREAp-TMENTvalue(Let7bS) - negativLog2 Reatio control1) (Let7b - negativp-value(Let7be control1) - negativLog2 Reatio control2) (Let7b - negative control2) aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) AKR1D1 NM_005989 3.28E-12 2.52E-12 -3.85007 3.59E-12 -3.73727 lin-28 homolog B (C. elegans) LIN28B NM_001004317 6.13E-15 8.29E-15 -3.29879 1.55E-15 -3.79656 high mobility group AT-hook 2 /// high mobility group AT-hook 2 HMGA2 NM_001015886 /// NM_0034833.74E-14 /// NM_0034844.29E-14 -3.06085 4.56E-14 -3.04538 HECT, C2 and WW domain containing E3 ubiquitin 2 HECW2 NM_020760 1.27E-13 6.65E-13 -2.94724 4.47E-12 -2.50907 cell division cycle 25A CDC25A NM_001789 /// NM_2015672.01E-11 7.32E-11 -2.88831 1.99E-11 -3.22735 hypothetical protein FLJ21986 FLJ21986 NM_024913 1.05E-09 5.19E-10 -2.80277 1.18E-09 -2.61084 2 (facilitated glucose transporter), member 3 SLC2A3 NM_006931 1.59E-13 3.49E-13 -2.78111 1.84E-12 -2.41734 Transcribed ------2.58E-13 1.08E-13 -2.59794 1.69E-13 -2.50248 Hypothetical protein LOC145786 LOC145786 --- 4.23E-12 1.07E-11 -2.58849 3.00E-12 -2.88135 Dicer1, Dcr-1 homolog (Drosophila) DICER1 NM_030621 /// NM_1774381.06E-08 4.37E-09 -2.5442 4.49E-09 -2.53796 mannose-binding lectin (protein C) 2, soluble (opsonic defect) MBL2 NM_000242 9.73E-10 1.48E-09 -2.53211 9.84E-10 -2.62363 cell division cycle 25A CDC25A NM_001789 /// NM_2015677.44E-14 8.65E-14 -2.49891 3.90E-14 -2.67164 hypothetical protein LOC145786 LOC145786 --- 9.68E-11 2.10E-10 -2.49462 6.21E-11 -2.7689 Dicer1, Dcr-1 homolog (Drosophila) DICER1 NM_030621 /// NM_1774382.79E-14 2.51E-14 -2.45436 3.21E-14 -2.40416 haloacid dehalogenase-like domain containing 1A HDHD1A NM_012080 9.61E-15 1.00E-14 -2.44389 1.23E-14 -2.40192 Dicer1, Dcr-1 homolog (Drosophila) DICER1 NM_030621 /// NM_1774384.46E-08 2.78E-08 -2.43541 1.49E-08 -2.57479 dual specificity phosphatase 9 DUSP9 NM_001395 8.85E-13 7.63E-13 -2.42489 6.76E-13 -2.4497 solute carrier organic anion transporter family, member 4C1 SLCO4C1 NM_180991 4.23E-10 4.73E-10 -2.41257 9.91E-11 -2.75897 solute carrier family 2 (facilitated glucose transporter), member 3 SLC2A3 NM_006931 6.99E-14 2.93E-13 -2.37509 1.06E-12 -2.13093 hyaluronan-mediated motility receptor (RHAMM) HMMR NM_012484 /// NM_0124851.53E-11 1.48E-11 -2.35937 8.98E-12 -2.46154 Putative neuronal cell adhesion molecule PUNC NM_004884 1.13E-08 1.31E-08 -2.33991 2.42E-08 -2.21604 aurora kinase B AURKB NM_004217 1.04E-11 9.53E-12 -2.29164 8.71E-12 -2.30913 solute carrier family 2 (facilitated glucose transporter), member 3 /// solute c SLC2A3 /// SLC2A14NM_006931 /// NM_1534492.53E-11 1.17E-10 -2.28343 3.73E-10 -2.06781 pleiomorphic adenoma gene-like 2 PLAGL2 NM_002657 2.13E-12 1.62E-12 -2.25936 1.43E-12 -2.28277 cyclin A2 CCNA2 NM_001237 5.19E-13 4.88E-13 -2.24473 6.29E-13 -2.19724 ubiquitin-like, containing PHD and RING finger domains, 1 UHRF1 NM_013282 6.16E-14 1.70E-13 -2.22165 2.84E-13 -2.12743 tubulin tyrosine ligase-like family, member 4 TTLL4 NM_014640 1.97E-14 1.49E-14 -2.15645 7.76E-15 -2.27775 similar to RIKEN cDNA 1200014N16 gene MGC14289 NM_080660 3.62E-13 7.78E-13 -2.14115 6.29E-13 -2.17971 E2F transcription factor 5, p130-binding E2F5 NM_001951 1.92E-13 1.19E-13 -2.12946 5.24E-13 -1.8803 CDC6 cell division cycle 6 homolog (S. cerevisiae) CDC6 NM_001254 4.82E-13 8.52E-12 -2.12602 7.53E-12 -2.14828 deiodinase, iodothyronine, type I DIO1 NM_000792 /// NM_2135931.70E-09 3.42E-09 -2.123 1.02E-09 -2.35815 denticleless homolog (Drosophila) DTL NM_016448 4.35E-11 6.78E-10 -2.07975 9.03E-10 -2.02875 RAD18 homolog (S. cerevisiae) RAD18 NM_020165 6.73E-09 6.63E-09 -2.07149 5.77E-09 -2.09708 serine/threonine kinase 6 STK6 NM_003600 /// NM_1984332.06E-13 /// NM_1984344.75E-13 /// NM_198435 ///-2.06623 NM_198436 /// 6.77E-13NM_198437 -2.00555 S-phase kinase-associated protein 2 (p45) SKP2 NM_005983 /// NM_0326371.46E-11 3.79E-10 -2.0565 1.90E-10 -2.18265 dopa decarboxylase (aromatic L-amino acid decarboxylase) DDC NM_000790 6.92E-14 3.77E-14 -2.05118 4.33E-14 -2.02728 APin protein APIN NM_017855 7.25E-11 5.75E-11 -2.04877 3.38E-11 -2.14365 DNA replication complex GINS protein PSF2 Pfs2 NM_016095 7.74E-14 3.73E-13 -2.02611 8.01E-13 -1.89993 activator of S phase kinase ASK NM_006716 7.22E-12 4.16E-12 -2.02005 3.28E-12 -2.061 solute carrier family 2 (facilitated glucose transporter), member 3 /// solute c SLC2A3 /// SLC2A14NM_006931 /// NM_1534492.55E-13 3.64E-13 -2.01819 2.97E-12 -1.69067 solute carrier family 35, member F5 SLC35F5 NM_025181 9.35E-11 3.24E-11 -2.01487 6.50E-11 -1.89889 ribonucleotide reductase M2 polypeptide RRM2 NM_001034 2.28E-15 4.66E-15 -2.01117 2.93E-15 -2.09111 CDNA FLJ11381 fis, clone HEMBA1000501 ------3.88E-09 3.07E-09 -2.01062 2.27E-09 -2.06382 hypermethylated in cancer 2 HIC2 NM_015094 1.37E-08 8.34E-09 -2.01005 4.96E-09 -2.1038 Galactosidase, beta 1 GLB1 NM_000404 1.29E-10 1.15E-10 -2.00307 3.31E-11 -2.22704 denticleless homolog (Drosophila) DTL NM_016448 6.33E-15 1.38E-13 -1.99945 9.77E-14 -2.05806 solute carrier family 2 (facilitated glucose transporter), member 3 SLC2A3 NM_006931 2.27E-12 2.57E-12 -1.99809 1.58E-11 -1.71314 GAJ protein GAJ NM_032117 5.28E-11 5.62E-11 -1.99004 3.34E-11 -2.0803 hypothetical protein LOC253842 LOC253842 --- 5.23E-10 6.50E-10 -1.98709 1.90E-10 -2.20856 putative small membrane protein NID67 NID67 NM_032947 1.09E-08 5.41E-09 -1.9577 5.69E-09 -1.9489 hyaluronan-mediated motility receptor (RHAMM) HMMR NM_012484 /// NM_0124857.93E-10 7.69E-10 -1.9564 3.72E-10 -2.08276 hypermethylated in cancer 2 HIC2 NM_015094 5.01E-11 6.78E-11 -1.95061 9.35E-11 -1.89784 Paternally expressed 3 PEG3 NM_006210 2.40E-09 2.51E-08 -1.94183 1.45E-07 -1.65871 polymerase (DNA directed), epsilon 2 (p59 subunit) POLE2 NM_002692 6.07E-14 8.40E-14 -1.93668 7.40E-14 -1.95732 proteasome (prosome, macropain) activator subunit 4 PSME4 NM_014614 9.93E-07 4.19E-05 -1.93116 2.76E-05 -2.01839 lysyl oxidase LOX NM_002317 8.52E-11 4.31E-08 -1.9294 6.44E-08 -1.8609 zinc finger, CSL-type containing 2 ZCSL2 NM_206831 8.86E-12 1.11E-11 -1.92796 4.35E-12 -2.08693 paternally expressed 3 PEG3 NM_006210 4.00E-09 1.69E-07 -1.92223 3.93E-07 -1.77804 phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) /// p PIGA NM_002641 /// NM_0204721.47E-09 /// NM_0204736.90E-10 -1.91851 7.63E-10 -1.90193 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

14 open reading frame 46 C14orf46 NM_001024674 5.54E-11 2.76E-11 -1.91644 6.40E-11 -1.78396 chromosome 7 open reading frame 23 C7orf23 NM_024315 3.17E-13 1.22E-13 -1.916 3.48E-13 -1.75473 solute carrier family 39 (zinc transporter), member 14 SLC39A14 NM_015359 7.43E-06 0.000232616 -1.88947 0.000491779 -1.72679 family with sequence similarity 54, member A FAM54A NM_138419 2.76E-13 4.73E-13 -1.8867 5.36E-13 -1.86698 microtubule associated serine/threonine kinase-like MASTL NM_032844 6.54E-11 1.15E-10 -1.88332 5.66E-11 -2.00108 suppressor of variegation 3-9 homolog 2 (Drosophila) SUV39H2 NM_024670 3.71E-11 2.14E-11 -1.87873 1.38E-11 -1.95024 hypothetical protein MGC13204 MGC13204 NM_031465 1.93E-09 9.57E-10 -1.87292 9.86E-10 -1.8682 apolipoprotein B (including Ag(x) antigen) APOB NM_000384 5.43E-05 0.0286114 -1.86773 0.0284021 -1.87074 ribonucleotide reductase M2 polypeptide RRM2 NM_001034 1.98E-11 1.68E-10 -1.86737 2.02E-10 -1.83802 nucleoporin 98kDa NUP98 NM_005387 /// NM_0163202.33E-12 /// NM_1391312.98E-12 /// NM_139132 -1.86591 4.20E-12 -1.81269 solute carrier family 6 (amino acid transporter), member 14 SLC6A14 NM_007231 1.94E-11 3.56E-09 -1.86071 2.02E-07 -1.29761 MCM10 minichromosome maintenance deficient 10 (S. cerevisiae) MCM10 NM_018518 /// NM_1827511.82E-12 2.79E-11 -1.85807 2.49E-11 -1.87586 ring finger and WD repeat domain 3 RFWD3 NM_018124 2.04E-11 1.60E-11 -1.85037 1.26E-11 -1.8882 coproporphyrinogen oxidase CPOX NM_000097 4.48E-11 5.67E-11 -1.84531 5.30E-11 -1.85604 cell division cycle associated 7 /// cell division cycle associated 7 CDCA7 NM_031942 /// NM_1458101.16E-10 7.53E-11 -1.84512 2.41E-10 -1.67017 LSM6 homolog, U6 small nuclear RNA associated (S. cerevisiae) LSM6 NM_007080 1.18E-10 6.77E-11 -1.83721 6.61E-11 -1.84092 CDC6 cell division cycle 6 homolog (S. cerevisiae) CDC6 NM_001254 1.07E-11 3.08E-10 -1.8149 2.57E-10 -1.84322 orosomucoid 1 ORM1 NM_000607 3.05E-12 2.46E-12 -1.81461 1.38E-12 -1.90468 ADP-ribosylation-like factor 6 interacting protein 6 ARL6IP6 NM_152522 2.33E-11 1.39E-10 -1.81302 3.29E-11 -2.05023 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide SIAT7B NM_006456 6.67E-15 7.75E-15 -1.80597 9.83E-15 -1.77043 dopa decarboxylase (aromatic L-amino acid decarboxylase) DDC NM_000790 1.46E-08 5.87E-09 -1.80268 8.06E-09 -1.75315 fatty acid binding protein 1, liver FABP1 NM_001443 7.98E-08 4.29E-07 -1.7991 4.44E-08 -2.21205 CDNA clone IMAGE:3462401 ------6.48E-11 4.75E-11 -1.79875 6.79E-11 -1.74481 2'-phosphodiesterase 2'-PDE NM_177966 3.68E-09 1.67E-09 -1.79628 5.48E-09 -1.61967 hypothetical protein MGC23909 MGC23909 NM_174909 6.90E-12 6.59E-11 -1.7848 4.53E-11 -1.84267 IGF-II mRNA-binding protein 1 IMP-1 NM_006546 8.65E-12 3.82E-12 -1.77639 3.59E-12 -1.78557 orosomucoid 2 ORM2 NM_000608 3.24E-11 1.80E-11 -1.77516 1.41E-11 -1.81206 replication factor C (activator 1) 5, 36.5kDa RFC5 NM_007370 /// NM_1815789.73E-11 1.08E-09 -1.76989 1.12E-09 -1.76349 serine/threonine kinase 6 STK6 NM_003600 /// NM_1984332.51E-15 /// NM_1984348.24E-15 /// NM_198435 ///-1.75624 NM_198436 /// 6.59E-15NM_198437 -1.78948 ------2.53E-12 5.69E-12 -1.74308 4.64E-12 -1.77329 signal peptidase complex subunit 3 homolog (S. cerevisiae) SPCS3 NM_021928 1.80E-09 4.90E-09 -1.74082 4.97E-09 -1.73859 phosphatidylinositol-4-phosphate 5-kinase, type I, beta PIP5K1B NM_001031687 /// NM_0035582.61E-10 9.16E-09 -1.73794 8.42E-09 -1.75078 hypothetical LOC83640 MGC2560 NM_031452 7.26E-12 2.52E-11 -1.73731 7.80E-11 -1.57759 zinc finger, CCHC domain containing 9 ZCCHC9 NM_032280 5.69E-13 2.81E-13 -1.73542 2.17E-13 -1.77374 CDNA FLJ26015 fis, clone MPC03596 ------8.53E-12 5.10E-12 -1.7338 9.59E-12 -1.64358 defective in sister chromatid cohesion homolog 1 (S. cerevisiae) DCC1 NM_024094 3.49E-09 3.63E-09 -1.72983 4.65E-09 -1.69285 S-phase kinase-associated protein 2 (p45) SKP2 NM_005983 /// NM_0326371.00E-13 1.32E-12 -1.71837 9.51E-13 -1.76707 RNA pseudouridylate synthase domain containing 3 RPUSD3 NM_173659 2.13E-11 9.20E-12 -1.71387 1.45E-11 -1.64924 MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) MCM4 NM_005914 /// NM_1827462.37E-07 6.03E-06 -1.71216 2.46E-05 -1.48451 Fidgetin FIGN NM_018086 5.33E-09 2.90E-09 -1.70097 1.85E-09 -1.76839 cyclin J CCNJ NM_019084 5.53E-09 4.98E-08 -1.69896 5.15E-08 -1.69376 E2F transcription factor 8 E2F8 NM_024680 6.03E-09 9.17E-09 -1.69216 9.81E-09 -1.68216 DNA replication complex GINS protein PSF1 PSF1 NM_021067 7.16E-13 1.15E-12 -1.68998 6.18E-13 -1.78035 WD repeat and HMG-box DNA binding protein 1 WDHD1 NM_001008396 /// NM_0070863.17E-07 8.99E-07 -1.68423 5.58E-07 -1.76124 heterogeneous nuclear ribonucleoprotein C (C1/C2) HNRPC NM_004500 /// NM_0313141.83E-10 9.18E-11 -1.68092 6.61E-11 -1.72875 Cyclin A2 CCNA2 NM_001237 6.13E-10 9.26E-10 -1.6807 4.01E-10 -1.8065 Polymerase (RNA) III (DNA directed) polypeptide G (32kD) POLR3G NM_006467 3.07E-10 1.29E-08 -1.67197 6.67E-09 -1.77231 BRCA2 and CDKN1A interacting protein BCCIP NM_016567 /// NM_0784682.02E-11 /// NM_0784698.33E-12 -1.67179 1.76E-11 -1.56936 CHK1 checkpoint homolog (S. pombe) CHEK1 NM_001274 2.66E-11 1.96E-10 -1.66818 1.28E-10 -1.73053 Full-length cDNA clone CS0DL007YI24 of B cells (Ramos cell line) Cot 25-normaliz ------2.92E-12 7.44E-12 -1.66407 2.93E-12 -1.80035 leucine zipper protein FKSG14 FKSG14 NM_022145 2.26E-08 1.37E-08 -1.65982 1.83E-08 -1.6174 hypothetical protein BC007901 LOC91461 NM_138370 7.83E-13 4.70E-13 -1.6546 1.49E-12 -1.50124 chromosome 9 open reading frame 41 C9orf41 NM_152420 3.10E-09 9.98E-09 -1.64783 1.78E-08 -1.56538 replication factor C (activator 1) 3, 38kDa RFC3 NM_002915 /// NM_1815582.88E-10 5.10E-10 -1.64637 3.61E-10 -1.696 TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa TAF5 NM_006951 2.83E-10 1.28E-10 -1.64589 1.12E-10 -1.66496 downstream neighbor of SON DONSON NM_017613 /// NM_1457947.38E-11 /// NM_1457957.29E-11 -1.64562 8.53E-11 -1.62363 breast cancer 1, early onset BRCA1 NM_007294 /// NM_0072951.74E-11 /// NM_0072961.31E-10 /// NM_007297 ///-1.64551 NM_007298 /// 3.48E-10NM_007299 -1.51353 breast cancer 2, early onset BRCA2 NM_000059 1.17E-10 2.29E-09 -1.64461 7.41E-10 -1.81395 lectin, galactoside-binding, soluble, 2 (galectin 2) /// lectin, galactoside-bin LGALS2 NM_006498 3.57E-12 2.95E-09 -1.64449 4.60E-10 -1.93185 orosomucoid 1 /// orosomucoid 2 ORM1 /// ORM2 NM_000607 /// NM_0006084.71E-13 2.98E-13 -1.64094 2.29E-13 -1.67779 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

kelch repeat and BTB (POZ) domain containing 8 KBTBD8 NM_032505 2.94E-07 3.06E-07 -1.63778 8.11E-08 -1.84904 Transcribed locus ------5.77E-10 2.61E-10 -1.63139 2.50E-10 -1.63782 kelch-like 14 (Drosophila) KLHL14 NM_020805 8.69E-11 1.16E-09 -1.6273 1.47E-09 -1.59445 Transforming growth factor, beta receptor III (betaglycan, 300kDa) TGFBR3 NM_003243 8.19E-15 4.22E-12 -1.6208 2.92E-12 -1.67202 ATPase family, AAA domain containing 2 ATAD2 NM_014109 4.37E-10 1.59E-09 -1.6198 2.32E-09 -1.56741 vaccinia related kinase 1 VRK1 NM_003384 1.40E-08 1.13E-08 -1.61929 5.56E-09 -1.72331 regulatory solute carrier protein, family 1, member 1 RSC1A1 NM_006511 1.40E-09 7.16E-10 -1.6183 5.01E-10 -1.66913 solute carrier family 16 (monocarboxylic acid transporters), member 10 SLC16A10 NM_018593 1.32E-09 4.41E-09 -1.61765 5.63E-09 -1.58333 hypothetical protein LOC201725 LOC201725 NM_001008393 3.53E-10 8.47E-09 -1.61568 1.77E-08 -1.51379 geminin, DNA replication inhibitor GMNN NM_015895 8.00E-13 3.18E-13 -1.611 3.78E-13 -1.58766 S-phase kinase-associated protein 2 (p45) SKP2 NM_005983 /// NM_0326372.17E-10 6.20E-09 -1.60746 2.66E-09 -1.73114 replication factor C (activator 1) 5, 36.5kDa RFC5 NM_007370 /// NM_1815782.70E-13 2.23E-12 -1.60502 1.40E-12 -1.67008 syntaxin 3A STX3A NM_004177 2.53E-06 7.98E-07 -1.60047 1.27E-06 -1.53116 NIMA (never in mitosis gene a)-related kinase 3 /// NIMA (never in mitosis gene NEK3 NM_002498 /// NM_1527202.81E-10 4.56E-10 -1.59866 4.47E-10 -1.60159 Cbl-interacting protein Sts-1 STS-1 NM_032873 2.21E-10 1.42E-10 -1.59182 7.44E-11 -1.68209 phosphatidylcholine transfer protein PCTP NM_021213 1.77E-10 7.49E-11 -1.58354 8.72E-11 -1.563 hypothetical protein MGC13204 MGC13204 NM_031465 1.77E-10 7.03E-11 -1.58036 8.03E-11 -1.56246 MHC class I polypeptide-related sequence B MICB NM_005931 3.41E-11 1.44E-09 -1.57712 2.42E-10 -1.83939 exportin 4 XPO4 NM_022459 1.22E-09 1.09E-09 -1.57449 5.24E-10 -1.67676 Zwilch, kinetochore associated, homolog (Drosophila) ZWILCH NM_017975 4.60E-09 3.57E-09 -1.56525 2.07E-09 -1.64142 ATPase family, AAA domain containing 2 ATAD2 NM_014109 3.38E-11 2.52E-10 -1.56497 1.56E-10 -1.63031 cell division cycle 34 CDC34 NM_004359 2.37E-12 7.59E-13 -1.56364 2.26E-12 -1.42634 nuclear receptor subfamily 1, group H, member 4 NR1H4 NM_005123 1.75E-13 3.19E-10 -1.56265 1.36E-10 -1.68099 MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) MCM4 NM_005914 /// NM_1827461.26E-09 1.60E-09 -1.5552 3.84E-09 -1.44129 solute carrier family 25, member 32 /// solute carrier family 25, member 32 SLC25A32 NM_030780 3.25E-10 2.18E-10 -1.55473 5.43E-10 -1.43752 NFKB inhibitor interacting Ras-like 1 NKIRAS1 NM_020345 2.30E-10 2.53E-10 -1.55409 5.12E-11 -1.78176 uncharacterized bone marrow protein BM039 BM039 NM_018455 2.87E-07 2.16E-07 -1.54953 3.25E-07 -1.49244 CDNA FLJ41999 fis, clone SPLEN2029683 ------1.39E-12 7.11E-13 -1.54495 7.30E-13 -1.54165 IGF-II mRNA-binding protein 1 IMP-1 NM_006546 7.01E-09 2.77E-09 -1.54188 3.13E-09 -1.52555 RAD51 associated protein 1 RAD51AP1 NM_006479 1.42E-09 1.33E-08 -1.53953 9.89E-09 -1.58022 Helicase, lymphoid-specific HELLS NM_018063 4.82E-09 7.59E-09 -1.53755 3.17E-08 -1.35487 ubiquitin carboxyl-terminal hydrolase L5 UCHL5 NM_015984 2.07E-10 8.98E-10 -1.53639 6.13E-10 -1.58781 DEP domain containing 1 DEPDC1 NM_017779 3.35E-11 3.72E-09 -1.52998 9.28E-10 -1.72629 MRS2-like, magnesium homeostasis factor (S. cerevisiae) MRS2L NM_020662 3.58E-12 1.01E-11 -1.52887 9.64E-12 -1.53449 family with sequence similarity 72, member A FAM72A NM_207418 9.03E-09 4.74E-09 -1.5278 3.69E-09 -1.56173 ATPase family, AAA domain containing 2 ATAD2 NM_014109 7.45E-08 3.85E-07 -1.52541 3.75E-06 -1.2289 mediator of RNA polymerase II transcription, subunit 6 homolog (yeast) MED6 NM_005466 1.90E-11 1.17E-11 -1.52471 1.26E-11 -1.51519 MLF1 interacting protein MLF1IP NM_024629 2.03E-10 8.86E-10 -1.52358 8.39E-10 -1.5308 timeless-interacting protein FLJ20516 NM_017858 6.28E-09 1.64E-08 -1.51974 4.37E-09 -1.70727 DNA replication factor CDT1 NM_030928 1.46E-11 2.83E-11 -1.5194 2.62E-11 -1.52933 CTP synthase CTPS NM_001905 6.52E-13 1.84E-13 -1.51909 7.51E-13 -1.34982 proliferation-associated 2G4, 38kDa PA2G4 NM_006191 1.07E-09 7.95E-10 -1.51548 8.38E-10 -1.5086 AT rich interactive domain 3A (BRIGHT- like) ARID3A NM_005224 3.54E-09 3.94E-09 -1.5126 7.73E-09 -1.42591 S100P binding protein Riken S100PBPR NM_001017406 /// NM_0227532.33E-09 8.18E-10 -1.51005 1.22E-09 -1.4586 helicase, lymphoid-specific HELLS NM_018063 6.51E-07 6.15E-07 -1.50777 9.21E-07 -1.4516 hypothetical protein FLJ20425 LYAR NM_017816 1.25E-10 5.19E-10 -1.5074 1.32E-09 -1.39083 chromosome 13 open reading frame 23 C13orf23 NM_025138 /// NM_1707196.71E-09 8.93E-09 -1.50348 2.60E-08 -1.36793 GIPC PDZ domain containing family, member 2 GIPC2 NM_017655 1.03E-06 4.24E-07 -1.50208 4.78E-07 -1.48568 dihydrofolate reductase DHFR NM_000791 7.31E-09 2.10E-07 -1.49665 2.30E-07 -1.48402 YOD1 OTU deubiquinating 1 homolog ( yeast) YOD1 NM_018566 2.06E-11 1.62E-08 -1.4894 1.58E-10 -2.22811 uncharacterized bone marrow protein BM039 BM039 NM_018455 2.40E-09 8.14E-10 -1.48869 1.30E-09 -1.42952 transforming growth factor, beta receptor III (betaglycan, 300kDa) TGFBR3 NM_003243 1.51E-12 4.91E-09 -1.48829 3.95E-09 -1.51703 MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisi MCM5 NM_006739 8.39E-10 9.41E-10 -1.48726 3.70E-09 -1.32021 TEA domain family member 4 TEAD4 NM_003213 /// NM_2014411.66E-10 /// NM_2014433.04E-10 -1.48692 6.16E-10 -1.39899 WW domain binding protein 11 WBP11 NM_016312 7.78E-10 2.64E-10 -1.48681 5.66E-10 -1.39236 putative neuronal cell adhesion molecule PUNC NM_004884 3.24E-10 1.08E-09 -1.48537 3.12E-09 -1.35466 PHD finger protein 19 PHF19 NM_001009936 /// NM_0156514.07E-08 8.93E-09 -1.48279 4.29E-08 -1.28983 MCM10 minichromosome maintenance deficient 10 (S. cerevisiae) MCM10 NM_018518 /// NM_1827514.42E-09 4.49E-07 -1.4752 2.59E-07 -1.55219 DKFZP434B0335 protein DKFZP434B0335 NM_015395 2.85E-07 4.43E-06 -1.47374 1.38E-05 -1.31602 mediator of RNA polymerase II transcription, subunit 6 homolog (yeast) MED6 NM_005466 8.31E-11 4.27E-11 -1.4714 5.82E-11 -1.43295 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

mal, T-cell differentiation protein 2 MAL2 NM_052886 2.46E-08 1.45E-08 -1.47044 8.60E-08 -1.25433 Full-length cDNA clone CL0BB014ZH04 of Neuroblastoma of Homo sapiens (human) ------2.75E-09 2.45E-09 -1.47016 1.16E-09 -1.56901 similar to RIKEN cDNA 2510006C20 gene LOC494143 NM_001008708 1.95E-11 3.51E-09 -1.46994 4.39E-09 -1.44145 metal response element binding transcription factor 2 MTF2 NM_007358 5.04E-08 2.34E-08 -1.46975 2.03E-08 -1.4888 hypothetical protein MGC4308 /// hypothetical protein MGC4308 MGC4308 NM_032359 2.17E-10 8.58E-11 -1.46946 1.96E-10 -1.36913 cell division cycle associated 2 CDCA2 NM_152562 4.76E-09 7.23E-09 -1.46591 4.24E-09 -1.53616 NIMA (never in mitosis gene a)-related kinase 3 NEK3 NM_002498 /// NM_1527201.56E-11 9.21E-12 -1.46462 2.30E-11 -1.35497 Full length insert cDNA YP99D02 ------5.07E-13 2.06E-13 -1.4627 2.32E-13 -1.44841 chromosome 9 open reading frame 41 C9orf41 NM_152420 2.71E-07 2.69E-06 -1.45984 7.74E-06 -1.31601 GrpE-like 1, mitochondrial (E. coli) GRPEL1 NM_025196 3.67E-13 1.63E-13 -1.45809 2.63E-13 -1.40063 ELL associated factor 2 EAF2 NM_018456 4.66E-11 9.97E-11 -1.45638 4.79E-11 -1.55047 cyclin E2 CCNE2 NM_057735 /// NM_0577493.06E-09 1.15E-07 -1.45261 1.37E-08 -1.75719 RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) RAD51 NM_002875 /// NM_1334873.66E-11 3.05E-09 -1.45245 3.21E-09 -1.44586 lysyl oxidase LOX NM_002317 2.71E-08 6.22E-05 -1.45127 4.59E-05 -1.49963 lamin B1 LMNB1 NM_005573 1.76E-11 3.07E-09 -1.45019 4.89E-09 -1.39214 ADP-ribosylation-like factor 6 interacting protein 6 ARL6IP6 NM_152522 1.26E-10 6.79E-11 -1.44979 5.76E-11 -1.47035 non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase) NME6 NM_005793 7.56E-10 4.39E-10 -1.44865 3.21E-10 -1.48807 hypothetical protein FLJ32745 FLJ32745 NM_144978 1.87E-11 2.24E-10 -1.44844 1.02E-10 -1.54865 nucleoporin 35kDa NUP35 NM_001008544 /// NM_1382854.73E-10 8.99E-10 -1.44823 8.41E-11 -1.77534 regulator of G-protein signalling 3 RGS3 NM_017790 /// NM_0211062.52E-09 /// NM_1307951.58E-09 /// NM_134427 ///-1.43884 NM_144488 /// 1.08E-09NM_144489 -1.48736 poly(A) binding protein, cytoplasmic 4 (inducible form) PABPC4 NM_003819 4.84E-14 1.90E-14 -1.43675 2.49E-14 -1.40467 NMDA receptor regulated 1 NARG1 NM_057175 4.13E-06 8.60E-06 -1.43307 5.77E-06 -1.49124 myofibrillogenesis regulator 1 MR-1 NM_015488 /// NM_0225723.81E-13 5.76E-13 -1.43073 3.63E-13 -1.48748 helicase, lymphoid-specific HELLS NM_018063 7.16E-10 1.02E-09 -1.42816 4.20E-09 -1.26281 translocated promoter region (to activated MET oncogene) TPR NM_003292 0.00126038 0.00332803 -1.42523 0.00901082 -1.21468 Protein kinase, cAMP-dependent, regulatory, type II, alpha PRKAR2A NM_004157 2.42E-12 9.70E-13 -1.42468 1.32E-12 -1.38857 hypothetical protein MGC11102 MGC11102 NM_032325 9.21E-11 8.19E-11 -1.42194 5.30E-11 -1.47567 sterol O-acyltransferase 2 SOAT2 NM_003578 3.98E-11 1.07E-09 -1.4215 1.07E-09 -1.42131 Dual specificity phosphatase 7 DUSP7 NM_001947 4.48E-11 1.18E-11 -1.42048 3.45E-11 -1.29669 Mesenchymal stem cell protein DSC96 ------5.15E-07 1.12E-06 -1.41895 2.17E-07 -1.65274 cyclin J CCNJ NM_019084 1.83E-09 3.55E-08 -1.41875 3.72E-08 -1.41267 TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa TAF5 NM_006951 1.37E-07 6.23E-08 -1.41728 5.48E-08 -1.43365 Early growth response 1 EGR1 NM_001964 1.95E-08 3.78E-07 -1.41406 7.40E-07 -1.32805 Fanconi anemia, complementation group D2 FANCD2 NM_001018115 /// NM_0330843.18E-09 7.34E-09 -1.41106 8.33E-09 -1.3955 G protein-coupled receptor 157 GPR157 NM_024980 1.49E-12 3.31E-12 -1.40952 7.38E-13 -1.59972 origin recognition complex, subunit 1-like (yeast) ORC1L NM_004153 1.12E-09 4.66E-09 -1.40886 6.51E-09 -1.36808 KIAA1702 protein KIAA1702 --- 5.78E-07 3.81E-06 -1.40858 1.91E-06 -1.50601 sorting nexin 5 SNX5 NM_014426 /// NM_1522272.77E-14 1.07E-14 -1.40817 1.32E-14 -1.38401 CDNA clone IMAGE:5301910 ------3.21E-08 9.90E-08 -1.40156 3.58E-08 -1.53593 NMDA receptor regulated 1 NARG1 NM_057175 2.05E-07 1.66E-07 -1.40091 5.28E-08 -1.55414 DNA replication factor CDT1 NM_030928 1.71E-09 6.92E-09 -1.40076 3.24E-09 -1.49693 chromosome condensation protein G HCAP-G NM_022346 2.30E-09 9.13E-08 -1.40035 4.44E-08 -1.4944 MCM10 minichromosome maintenance deficient 10 (S. cerevisiae) MCM10 NM_018518 /// NM_1827514.70E-12 4.72E-11 -1.39848 5.34E-11 -1.38398 Cyclin-dependent kinase 8 CDK8 NM_001260 3.14E-12 1.44E-10 -1.39665 2.31E-10 -1.34148 ankyrin repeat domain 32 ANKRD32 NM_032290 3.43E-09 1.10E-08 -1.3931 1.32E-08 -1.37147 progesterone receptor membrane component 1 PGRMC1 NM_006667 4.47E-13 1.69E-13 -1.39165 2.75E-13 -1.33545 microsomal triglyceride transfer protein (large polypeptide, 88kDa) MTP NM_000253 5.52E-11 5.00E-11 -1.39116 1.29E-11 -1.5616 mesoderm development candidate 1 MESDC1 NM_022566 9.25E-13 9.45E-13 -1.39016 1.10E-12 -1.37282 gem (nuclear organelle) associated protein 7 GEMIN7 NM_001007269 /// NM_0010072705.25E-12 /// NM_0247071.70E-12 -1.38825 3.03E-12 -1.32234 activin A receptor, type IB ACVR1B NM_004302 /// NM_0203276.34E-09 /// NM_0203281.15E-08 -1.38593 5.40E-09 -1.48078 nuclear autoantigenic sperm protein (histone-binding) NASP NM_002482 /// NM_1522987.35E-12 /// NM_1721644.85E-12 -1.38458 1.09E-11 -1.29229 DEP domain containing 1B DEPDC1B NM_018369 4.22E-12 1.46E-09 -1.38299 1.89E-09 -1.35219 DAZ interacting protein 1 DZIP1 NM_014934 /// NM_1989689.36E-11 1.36E-10 -1.38091 2.16E-10 -1.32697 KIAA0179 KIAA0179 NM_015056 5.48E-12 1.18E-12 -1.38071 5.74E-12 -1.20797 Protein kinase, cAMP-dependent, regulatory, type II, alpha PRKAR2A NM_004157 8.47E-12 2.85E-12 -1.3798 4.89E-12 -1.31813 thymopoietin TMPO NM_001032283 /// NM_0010322842.77E-09 /// NM_0032762.38E-07 -1.37963 4.14E-07 -1.31101 ubiquitin-conjugating enzyme E2T (putative) UBE2T NM_014176 1.48E-09 1.50E-09 -1.37957 3.26E-10 -1.57373 baculoviral IAP repeat-containing 3 BIRC3 NM_001165 /// NM_1829627.50E-08 8.28E-08 -1.3791 1.62E-07 -1.29775 hypothetical protein LOC201725 LOC201725 NM_001008393 1.67E-08 1.43E-07 -1.37601 4.04E-07 -1.2509 melanoma cell adhesion molecule /// melanoma cell adhesion molecule MCAM NM_006500 8.11E-13 8.71E-12 -1.3743 8.68E-12 -1.37477 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

hypothetical protein FLJ10719 FLJ10719 NM_018193 2.10E-11 5.46E-11 -1.37392 4.87E-11 -1.38747 chromosome 18 open reading frame 24 C18orf24 NM_145060 7.01E-08 3.13E-07 -1.37389 1.66E-07 -1.4562 apolipoprotein C-III /// similar to Apolipoprotein C-III precursor (Apo-CIII) APOC3 /// LOC440838NM_000040 /// XM_4965372.82E-12 3.38E-10 -1.37091 1.12E-10 -1.50704 hypothetical protein FLJ10700 FLJ10700 NM_018182 7.65E-09 5.84E-09 -1.36877 1.07E-08 -1.29786 Ankyrin repeat domain 17 ANKRD17 NM_032217 /// NM_1988899.39E-12 2.11E-11 -1.36494 2.67E-11 -1.33775 polymerase (RNA) II (DNA directed) polypeptide D POLR2D NM_004805 1.24E-10 5.74E-11 -1.36082 5.93E-11 -1.35713 early growth response 1 EGR1 NM_001964 1.96E-10 3.30E-08 -1.36046 1.94E-08 -1.42625 PDZ binding kinase PBK NM_018492 1.87E-11 4.15E-10 -1.35963 1.91E-10 -1.4532 dystrophin (muscular dystrophy, Duchenne and Becker types) DMD NM_000109 /// NM_0040066.35E-07 /// NM_0040076.19E-07 /// NM_004009 ///-1.35531 NM_004010 /// 3.07E-07NM_004011 -1.44654 DEP domain containing 1 DEPDC1 NM_017779 1.59E-11 1.02E-09 -1.35062 3.48E-10 -1.48252 gem (nuclear organelle) associated protein 7 GEMIN7 NM_001007269 /// NM_0010072701.94E-10 /// NM_0247078.14E-11 -1.34927 8.61E-11 -1.34271 chromosome 1 open reading frame 112 C1orf112 NM_018186 2.56E-10 7.28E-09 -1.34926 3.67E-09 -1.43296 MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevis MCM6 NM_005915 1.24E-09 1.69E-09 -1.34875 4.17E-09 -1.24647 enhancer of zeste homolog 2 (Drosophila) EZH2 NM_004456 /// NM_1529985.36E-09 3.56E-08 -1.34523 4.07E-08 -1.32895 MAD2 mitotic arrest deficient-like 1 (yeast) MAD2L1 NM_002358 7.65E-12 7.90E-12 -1.34404 1.35E-11 -1.28422 KIAA1462 KIAA1462 XM_166132 1.11E-07 2.09E-07 -1.34317 3.03E-07 -1.29805 ATPase family, AAA domain containing 2 ATAD2 NM_014109 2.21E-08 4.62E-08 -1.34167 5.14E-08 -1.32877 ubiquitin specific peptidase 10 USP10 NM_005153 6.96E-07 9.28E-06 -1.34126 5.65E-05 -1.11161 chromosome condensation protein G HCAP-G NM_022346 1.07E-09 9.12E-08 -1.34047 2.41E-08 -1.51076 hypothetical protein LOC283464 LOC283464 XM_290597 1.20E-11 3.12E-10 -1.34039 1.58E-10 -1.42132 activator of basal transcription 1 ABT1 NM_013375 3.67E-11 9.77E-12 -1.33929 3.18E-11 -1.21164 hypothetical protein FLJ20425 LYAR NM_017816 5.21E-13 1.13E-12 -1.33826 5.83E-12 -1.1654 RNA binding motif protein 14 RBM14 NM_006328 4.66E-06 0.000298425 -1.33824 0.000920274 -1.1633 metal response element binding transcription factor 2 MTF2 NM_007358 1.36E-08 1.26E-08 -1.33747 2.65E-08 -1.25262 hypothetical protein MGC2408 MGC2408 NM_032331 6.55E-11 3.26E-11 -1.33663 2.59E-11 -1.36327 TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor TAF5L NM_001025247 /// NM_0144099.37E-10 2.12E-10 -1.33352 1.22E-09 -1.14619 solute carrier family 5 (sodium-dependent vitamin transporter), member 6 SLC5A6 NM_021095 7.90E-14 6.94E-14 -1.33027 5.05E-14 -1.36618 CDC45 cell division cycle 45-like (S. cerevisiae) CDC45L NM_003504 3.50E-11 5.92E-11 -1.32937 9.41E-11 -1.27788 serine/threonine kinase 6 STK6 NM_003600 /// NM_1984335.04E-07 /// NM_1984341.70E-06 /// NM_198435 ///-1.32801 NM_198436 /// 7.79E-06NM_198437 -1.14454 nucleolar and coiled-body phosphoprotein 1 NOLC1 NM_004741 6.97E-14 9.20E-14 -1.32785 8.24E-14 -1.34026 IGF-II mRNA-binding protein 2 IMP-2 NM_001007225 /// NM_0065484.41E-11 5.72E-11 -1.32673 6.43E-11 -1.3137 suppressor of Ty 16 homolog (S. cerevisiae) SUPT16H NM_007192 1.39E-05 0.000357168 -1.32309 0.00130031 -1.12258 replication factor C (activator 1) 3, 38kDa RFC3 NM_002915 /// NM_1815582.87E-11 6.31E-11 -1.32174 6.34E-11 -1.32119 MCM7 minichromosome maintenance deficient 7 (S. cerevisiae) MCM7 NM_005916 /// NM_1827769.43E-12 5.53E-11 -1.31903 5.86E-11 -1.31254 PHD finger protein 19 PHF19 NM_001009936 /// NM_0156516.14E-11 2.31E-11 -1.31829 5.86E-11 -1.21796 MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) MCM4 NM_005914 /// NM_1827462.36E-11 1.64E-10 -1.31801 7.99E-10 -1.15041 RNA, U47 small nuclear RNU47 XR_000223 3.31E-09 3.55E-09 -1.31604 2.11E-09 -1.37722 Mesenchymal stem cell protein DSC96 ------3.86E-08 1.06E-07 -1.31591 4.23E-08 -1.42921 nei endonuclease VIII-like 3 (E. coli) NEIL3 NM_018248 1.43E-13 1.11E-11 -1.3144 3.23E-12 -1.45953 solute carrier family 25, member 13 (citrin) SLC25A13 NM_014251 2.21E-12 8.87E-12 -1.31304 4.57E-12 -1.38888 CHK1 checkpoint homolog (S. pombe) CHEK1 NM_001274 1.57E-11 3.84E-10 -1.31258 1.59E-10 -1.41591 MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) MCM3 NM_002388 3.62E-12 2.46E-12 -1.31064 4.02E-12 -1.25731 polo-like kinase 4 (Drosophila) PLK4 NM_014264 4.28E-10 9.12E-10 -1.30833 6.01E-10 -1.35629 progesterone receptor membrane component 1 PGRMC1 NM_006667 1.45E-10 4.58E-11 -1.30484 8.78E-11 -1.23433 synaptosomal-associated protein, 23kDa SNAP23 NM_003825 /// NM_1307985.14E-09 3.08E-09 -1.30437 1.07E-08 -1.16959 hypothetical LOC131076 LOC131076 NM_001017928 6.57E-05 9.98E-05 -1.30257 6.41E-05 -1.36762 ubiquitin carboxyl-terminal hydrolase L5 UCHL5 NM_015984 6.46E-12 3.21E-11 -1.3016 1.39E-11 -1.39732 phosphoribosyl pyrophosphate amidotransferase PPAT NM_002703 2.31E-09 3.71E-09 -1.29917 2.49E-09 -1.34513 paternally expressed 3 PEG3 NM_006210 1.79E-10 9.38E-10 -1.29809 1.70E-09 -1.23259 mutS homolog 6 (E. coli) MSH6 NM_000179 1.70E-09 1.24E-09 -1.29595 1.14E-09 -1.30582 mitochondrial carrier protein MGC4399 NM_032315 2.12E-09 6.03E-10 -1.29462 2.53E-09 -1.14344 UDP-galactose-4-epimerase GALE NM_000403 /// NM_0010082162.99E-10 1.20E-10 -1.29186 1.45E-10 -1.27157 primase, polypeptide 1, 49kDa PRIM1 NM_000946 8.84E-11 7.83E-11 -1.2903 6.78E-11 -1.30614 thyroid hormone receptor interactor 13 TRIP13 NM_004237 2.85E-11 1.36E-11 -1.2897 5.45E-11 -1.14632 NY-REN-41 antigen NY-REN-41 NM_030771 /// NM_0806543.54E-11 1.51E-11 -1.28941 1.61E-11 -1.28257 chromosome 10 open reading frame 9 C10orf9 NM_145012 /// NM_1816982.56E-06 1.02E-06 -1.2867 1.48E-06 -1.24183 transferrin receptor (p90, CD71) TFRC NM_003234 1.13E-05 0.00149973 -1.28588 0.000805411 -1.39634 hypothetical protein FLJ10534 FLJ10534 NM_018128 1.67E-09 8.20E-10 -1.28555 1.34E-09 -1.23197 solute carrier family 25, member 13 (citrin) SLC25A13 NM_014251 6.22E-06 4.38E-06 -1.28509 1.83E-06 -1.3986 MCM4 minichromosome maintenance deficient 4 (S. cerevisiae) MCM4 NM_005914 /// NM_1827462.94E-10 4.00E-10 -1.28478 1.33E-09 -1.15782 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

similar to RIKEN cDNA 8030451K01 LOC387921 NM_001012754 /// NM_0010173703.89E-09 5.62E-09 -1.28425 9.20E-10 -1.50348 alanine-glyoxylate aminotransferase 2-like 1 /// alanine-glyoxylate aminotransfe AGXT2L1 NM_031279 2.48E-08 2.99E-08 -1.28226 3.30E-08 -1.27096 fibrillarin /// fibrillarin FBL NM_001436 4.91E-12 1.74E-12 -1.28206 2.61E-12 -1.23852 Treacher Collins-Franceschetti syndrome 1 TCOF1 NM_000356 /// NM_0010086565.99E-11 /// NM_0010086572.55E-11 -1.28176 2.57E-11 -1.28086 chromosome 13 open reading frame 23 C13orf23 NM_025138 /// NM_1707191.50E-10 1.88E-10 -1.2817 9.09E-11 -1.36413 maternal embryonic leucine zipper kinase MELK NM_014791 3.94E-12 1.05E-10 -1.28129 6.60E-11 -1.33305 hypothetical protein FLJ25416 FLJ25416 NM_145018 3.50E-08 1.28E-07 -1.2804 7.16E-08 -1.34943 transmembrane protein 48 TMEM48 NM_018087 1.58E-08 5.03E-08 -1.27882 2.21E-08 -1.37623 replication factor C (activator 1) 4, 37kDa RFC4 NM_002916 /// NM_1815732.15E-10 2.00E-10 -1.27759 7.32E-10 -1.14262 secretagogin, EF-hand calcium binding protein SCGN NM_006998 1.22E-08 2.00E-08 -1.27685 8.17E-09 -1.38172 neuroblastoma RAS viral (v-ras) oncogene homolog NRAS NM_002524 8.95E-08 7.06E-08 -1.27621 2.43E-08 -1.40417 Mix1 homeobox-like 1 (Xenopus laevis) MIXL1 NM_031944 1.38E-06 7.73E-07 -1.27466 2.61E-06 -1.1352 mitogen-activated protein kinase-activated protein kinase 5 MAPKAPK5 NM_003668 /// NM_1390781.41E-09 6.48E-10 -1.27438 8.28E-10 -1.24771 hypothetical protein FLJ13912 FLJ13912 NM_022770 7.92E-10 7.42E-10 -1.27405 1.06E-09 -1.23521 Fatty acid binding protein 1, liver FABP1 NM_001443 2.69E-07 6.76E-07 -1.27371 2.82E-07 -1.38165 FLJ34306 protein /// ADP-ribosylation factor-like LOC474170 /// LOC51326NM_001006607 /// NM_0166324.57E-07 4.00E-07 -1.27245 1.13E-07 -1.42861 hypothetical protein BC008207 LOC92345 NM_138386 1.63E-09 2.31E-09 -1.27234 2.97E-09 -1.24472 thioesterase superfamily member 4 THEM4 NM_053055 /// NM_1768532.90E-10 1.23E-10 -1.27062 1.94E-10 -1.22228 unc-93 homolog A (C. elegans) UNC93A NM_018974 3.22E-09 1.09E-09 -1.27045 3.71E-09 -1.14169 nucleosome assembly protein 1-like 1 NAP1L1 NM_004537 /// NM_1392079.64E-08 3.02E-08 -1.27045 5.58E-08 -1.20259 chromodomain helicase DNA binding protein 7 CHD7 NM_017780 9.76E-12 7.43E-12 -1.26578 8.24E-12 -1.2547 ------2.80E-08 1.59E-08 -1.25601 1.42E-08 -1.26833 polymerase (RNA) I polypeptide B, 128kDa POLR1B NM_019014 2.00E-09 1.91E-09 -1.25575 3.30E-09 -1.1974 deleted in lymphocytic leukemia, 2 /// BCMS upstream neighbor-like DLEU2 /// BCMSUNLNM_006021 7.47E-07 1.68E-06 -1.25515 6.29E-07 -1.37764 nuclear receptor subfamily 1, group H, member 4 NR1H4 NM_005123 6.99E-13 2.32E-09 -1.25513 6.11E-10 -1.40894 Polymerase (RNA) II (DNA directed) polypeptide D POLR2D NM_004805 8.31E-07 2.28E-07 -1.25503 5.31E-07 -1.16085 spindle assembly 6 homolog (C. elegans) SASS6 NM_194292 8.59E-09 1.15E-08 -1.25468 2.82E-09 -1.41968 phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetas GART NM_000819 /// NM_1750851.34E-06 5.61E-06 -1.25377 2.24E-06 -1.37124 metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA) MALAT1 --- 9.62E-05 0.00340115 -1.25317 0.000817983 -1.52678 DOT1-like, histone H3 methyltransferase (S. cerevisiae) DOT1L NM_032482 2.28E-05 3.22E-05 -1.25281 8.11E-06 -1.44315 phosphoribosyl pyrophosphate amidotransferase PPAT NM_002703 5.32E-11 9.78E-11 -1.25153 4.68E-11 -1.33285 MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae) MCM2 NM_004526 3.64E-11 2.29E-11 -1.25125 4.50E-11 -1.18124 MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisi MCM5 NM_006739 2.66E-09 1.95E-09 -1.24959 1.04E-08 -1.07948 WW domain binding protein 11 WBP11 NM_016312 1.04E-10 2.73E-11 -1.24956 1.01E-10 -1.11731 MCM8 minichromosome maintenance deficient 8 (S. cerevisiae) MCM8 NM_032485 /// NM_1828025.72E-08 1.88E-07 -1.24748 1.74E-07 -1.25619 guanine nucleotide binding protein (G protein), gamma 5 GNG5 NM_005274 1.74E-13 7.64E-14 -1.2442 7.30E-14 -1.24898 UTP15, U3 small nucleolar ribonucleoprotein, homolog (yeast) UTP15 NM_032175 1.02E-07 5.06E-08 -1.24301 8.94E-08 -1.18094 ATP-binding cassette, sub-family B (MDR/TAP), member 10 ABCB10 NM_012089 2.36E-08 1.39E-08 -1.24236 1.25E-08 -1.25435 IGF-II mRNA-binding protein 1 IMP-1 NM_006546 1.09E-10 6.14E-10 -1.24169 1.75E-10 -1.38299 fatty acid binding protein 5 (psoriasis-associated) FABP5 NM_001444 1.65E-08 1.14E-08 -1.24105 3.06E-08 -1.1368 Poly(rC) binding protein 2 PCBP2 NM_005016 /// NM_0319892.06E-09 1.05E-09 -1.24097 7.19E-10 -1.28254 chromosome 10 open reading frame 3 C10orf3 NM_018131 1.58E-10 2.80E-09 -1.24036 1.13E-09 -1.34268 SHC SH2-domain binding protein 1 SHCBP1 NM_024745 1.94E-10 1.72E-08 -1.23916 5.29E-09 -1.37479 hypothetical protein LOC253842 LOC253842 --- 3.10E-09 1.19E-07 -1.23869 7.21E-09 -1.59033 peptidylprolyl (cyclophilin)-like 5 PPIL5 NM_152329 /// NM_2034663.23E-10 /// NM_2034677.06E-10 -1.23645 6.12E-10 -1.25179 hypothetical protein FLJ10534 FLJ10534 NM_018128 1.01E-10 6.63E-11 -1.23607 6.36E-11 -1.24047 reticulon 4 interacting protein 1 RTN4IP1 NM_032730 3.99E-09 1.92E-09 -1.2337 2.18E-09 -1.22027 Matrin 3 MATR3 NM_018834 /// NM_1991891.46E-06 2.65E-06 -1.23311 2.75E-06 -1.22874 CDC28 protein kinase regulatory subunit 1B CKS1B NM_001826 3.07E-14 2.45E-13 -1.23303 1.03E-13 -1.32647 ADP-ribosylation factor-like 8 ARL8 NM_178815 1.30E-05 4.54E-06 -1.23207 6.04E-06 -1.19791 B2 (plasma, carboxypeptidase U) CPB2 NM_001872 /// NM_0164131.20E-08 1.02E-08 -1.23056 5.74E-09 -1.29482 leptin receptor overlapping transcript-like 1 LEPROTL1 NM_015344 3.72E-15 1.79E-13 -1.22955 2.91E-13 -1.1802 metal response element binding transcription factor 2 MTF2 NM_007358 7.04E-10 4.33E-10 -1.22897 3.28E-10 -1.25895 c-myc binding protein MYCBP NM_012333 5.68E-11 2.05E-09 -1.22862 4.20E-09 -1.15412 family with sequence similarity 57, member A FAM57A NM_024792 7.64E-11 3.50E-10 -1.22639 5.15E-10 -1.18624 mediator of RNA polymerase II transcription, subunit 8 homolog (yeast) MED8 NM_001001651 /// NM_0010016531.04E-10 /// NM_0010016544.95E-11 /// NM_052877-1.22443 /// NM_2015423.96E-11 -1.2479 BUB1 budding uninhibited by benzimidazoles 1 homolog beta (yeast) BUB1B NM_001211 2.06E-10 7.61E-10 -1.21978 9.32E-10 -1.19856 nucleolar and coiled-body phosphoprotein 1 NOLC1 NM_004741 5.27E-12 1.92E-11 -1.2188 2.41E-11 -1.19552 spindle pole body component 25 homolog (S. cerevisiae) SPBC25 NM_020675 1.76E-11 2.82E-09 -1.21597 5.74E-10 -1.39588 RNA, U47 small nuclear /// growth arrest-specific 5 RNU47 /// GAS5 NR_002578 /// XR_0002231.59E-09 3.21E-09 -1.21512 9.58E-10 -1.34963 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

DEP domain containing 1 DEPDC1 NM_017779 1.80E-11 6.99E-10 -1.21462 1.41E-10 -1.39394 60S ribosomal protein L6 (RPL6A) NAP1L1 NM_004537 /// NM_1392078.47E-07 2.54E-07 -1.21385 4.90E-07 -1.14208 Chromosome 9 open reading frame 52 C9orf52 NM_152574 1.03E-07 1.87E-07 -1.21367 7.58E-08 -1.31727 hypothetical protein HSPC111 HSPC111 NM_016391 4.95E-11 4.40E-10 -1.21326 3.89E-10 -1.22627 lamin B receptor LBR NM_002296 /// NM_1944427.47E-10 8.38E-10 -1.21212 6.41E-10 -1.24051 solute carrier family 35, member D2 SLC35D2 NM_007001 1.87E-10 7.20E-11 -1.21192 8.78E-11 -1.19155 E2F transcription factor 6 E2F6 NM_001952 /// NM_1982561.49E-12 /// NM_1982572.64E-12 /// NM_198258 ///-1.21168 NM_198325 /// 2.17E-12NM_212540 -1.23201 synovial sarcoma, X breakpoint 2 interacting protein SSX2IP NM_014021 3.16E-11 5.15E-11 -1.21119 9.59E-12 -1.39727 CDNA clone IMAGE:6043059 ------5.19E-09 1.64E-09 -1.21043 4.29E-09 -1.11343 ERO1-like (S. cerevisiae) ERO1L NM_014584 6.51E-12 2.49E-12 -1.21016 3.69E-12 -1.1706 cyclin B1 CCNB1 NM_031966 1.56E-08 5.68E-08 -1.20871 3.02E-07 -1.03823 F-box protein 5 FBXO5 NM_012177 1.47E-10 1.21E-09 -1.20725 1.60E-09 -1.17835 flap structure-specific endonuclease 1 FEN1 NM_004111 2.85E-12 2.86E-11 -1.20682 3.78E-11 -1.17879 tubulin, epsilon 1 TUBE1 NM_016262 1.12E-07 7.21E-08 -1.20665 7.78E-08 -1.19839 serine/threonine kinase 40 STK40 NM_032017 1.25E-08 1.11E-08 -1.20604 1.11E-08 -1.20579 kinesin family member 15 KIF15 NM_020242 5.17E-11 9.83E-10 -1.20465 1.35E-09 -1.17193 Cyclin-dependent kinase 8 CDK8 NM_001260 2.57E-10 2.92E-08 -1.20309 2.47E-08 -1.22143 synaptosomal-associated protein, 23kDa SNAP23 NM_003825 /// NM_1307986.92E-10 3.17E-10 -1.20178 2.62E-09 -1.00087 TAL1 (SCL) interrupting locus SIL NM_003035 3.73E-10 1.59E-08 -1.20159 4.62E-08 -1.09275 U2-associated SR140 protein SR140 XM_031553 4.03E-12 5.27E-12 -1.20146 3.89E-12 -1.23252 brix domain containing 2 BXDC2 NM_018321 1.59E-09 1.41E-09 -1.2003 9.94E-10 -1.23716 IGF-II mRNA-binding protein 2 IMP-2 NM_001007225 /// NM_0065481.21E-06 6.62E-07 -1.20028 5.92E-07 -1.21282 polo-like kinase 4 (Drosophila) /// polo-like kinase 4 (Drosophila) PLK4 NM_014264 1.55E-06 4.18E-05 -1.19979 1.66E-05 -1.32151 WD repeat and HMG-box DNA binding protein 1 WDHD1 NM_001008396 /// NM_0070862.14E-09 3.48E-09 -1.19807 2.20E-09 -1.24702 Cell division cycle 2, G1 to S and G2 to M CDC2 NM_001786 /// NM_0333791.79E-08 1.56E-08 -1.19468 8.72E-08 -1.02399 mitogen-activated protein kinase 6 MAPK6 NM_002748 6.19E-12 4.36E-11 -1.19468 2.11E-11 -1.27061 FLJ22624 protein FLJ22624 NM_024808 4.17E-09 1.38E-08 -1.19452 3.48E-09 -1.34847 CTSC NM_001814 /// NM_1481701.68E-09 1.54E-08 -1.19055 8.33E-08 -1.02371 YOD1 OTU deubiquinating enzyme 1 homolog ( yeast) YOD1 NM_018566 3.81E-11 7.87E-08 -1.18774 5.88E-10 -1.82783 Ribosomal protein S6 RPS6 NM_001010 3.18E-07 1.95E-05 -1.18712 1.31E-05 -1.23636 KIAA1287 KIAA1287 NM_020748 1.56E-08 3.95E-09 -1.18688 1.27E-08 -1.07139 thymidine kinase 1, soluble TK1 NM_003258 2.76E-10 8.41E-11 -1.18454 2.79E-10 -1.06864 phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinoca PAICS NM_006452 1.90E-09 3.36E-09 -1.1829 2.68E-09 -1.20665 cell division cycle 2, G1 to S and G2 to M CDC2 NM_001786 /// NM_0333791.18E-10 2.10E-10 -1.18188 6.64E-10 -1.07054 Retinoblastoma binding protein 7 RBBP7 NM_002893 5.60E-08 2.65E-08 -1.18172 2.07E-08 -1.20809 RAN binding protein 1 RANBP1 NM_002882 2.54E-11 1.91E-10 -1.18096 6.37E-10 -1.06476 replication factor C (activator 1) 2, 40kDa RFC2 NM_002914 /// NM_1814711.05E-09 1.63E-09 -1.1807 1.99E-09 -1.16022 cell division cycle associated 8 CDCA8 NM_018101 6.19E-10 7.50E-10 -1.17882 2.81E-10 -1.28276 chromosome 4 open reading frame 9 C4orf9 NM_003703 2.68E-11 8.91E-11 -1.17677 1.85E-10 -1.10543 Protein kinase, cAMP-dependent, regulatory, type II, alpha PRKAR2A NM_004157 3.16E-10 1.93E-10 -1.17498 1.40E-10 -1.20791 solute carrier family 43, member 1 SLC43A1 NM_003627 6.73E-08 2.75E-07 -1.17345 9.09E-08 -1.29822 pleiomorphic adenoma gene-like 2 PLAGL2 NM_002657 6.84E-07 1.74E-07 -1.16852 5.91E-07 -1.04352 Solute carrier family 39 (zinc transporter), member 14 SLC39A14 NM_015359 1.88E-07 3.92E-06 -1.16704 1.81E-06 -1.25794 Treacher Collins-Franceschetti syndrome 1 TCOF1 NM_000356 /// NM_0010086561.50E-08 /// NM_0010086571.19E-08 -1.16674 3.71E-09 -1.29254 dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransfera DPAGT1 NM_001382 /// NM_2033161.48E-13 4.94E-14 -1.16606 9.43E-14 -1.10439 solute carrier family 20 (phosphate transporter), member 1 SLC20A1 NM_005415 7.96E-15 1.02E-14 -1.16503 5.98E-15 -1.21825 hypothetical protein FLJ21986 FLJ21986 NM_024913 1.44E-09 5.70E-08 -1.16423 2.91E-07 -1.00377 thioesterase superfamily member 4 THEM4 NM_053055 /// NM_1768538.90E-11 3.08E-11 -1.16311 5.31E-11 -1.11024 vacuolar protein sorting 54 (yeast) VPS54 NM_001005739 /// NM_0165161.06E-11 1.20E-11 -1.16164 2.33E-12 -1.33426 hypothetical protein FLJ20364 FLJ20364 NM_017785 1.71E-11 4.21E-10 -1.1602 2.28E-10 -1.22326 flap structure-specific endonuclease 1 FEN1 NM_004111 1.42E-11 1.81E-10 -1.15972 1.63E-10 -1.1699 chromosome 1 open reading frame 33 C1orf33 NM_016183 1.17E-10 1.25E-10 -1.15913 9.60E-10 -0.972718 phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinoca PAICS NM_006452 9.59E-13 4.62E-13 -1.15803 8.53E-13 -1.09982 solute carrier family 22 (organic anion transporter), member 7 SLC22A7 NM_006672 /// NM_1533204.73E-07 3.32E-06 -1.15744 2.22E-06 -1.20341 heat shock 70kDa protein 14 HSPA14 NM_016299 1.10E-11 3.77E-11 -1.15728 7.20E-11 -1.09515 c-myc binding protein MYCBP NM_012333 1.21E-09 3.36E-06 -1.1569 6.08E-06 -1.09132 shugoshin-like 2 (S. pombe) SGOL2 NM_152524 1.65E-06 3.40E-05 -1.15689 1.78E-05 -1.23764 hypothetical protein MGC24665 MGC24665 NM_152308 3.92E-09 9.85E-10 -1.15665 3.28E-09 -1.04175 BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) BUB1 NM_004336 4.53E-13 3.75E-12 -1.15626 5.12E-12 -1.12615 cell division cycle associated 5 CDCA5 NM_080668 1.08E-11 1.16E-10 -1.15624 9.62E-11 -1.17481 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

fidgetin-like 1 FIGNL1 NM_022116 6.22E-07 2.12E-07 -1.15566 3.68E-07 -1.09838 glycerol-3-phosphate dehydrogenase 1 (soluble) GPD1 NM_005276 2.10E-10 1.88E-09 -1.15472 4.45E-10 -1.3084 cell division cycle 25A CDC25A NM_001789 /// NM_2015675.96E-10 5.69E-09 -1.15469 3.90E-10 -1.45719 early growth response 1 EGR1 NM_001964 1.78E-07 1.53E-06 -1.15428 1.31E-06 -1.17121 adenylate kinase 2 AK2 NM_001625 /// NM_0134111.01E-12 1.21E-12 -1.15317 1.12E-12 -1.16002 tubulin, gamma 1 TUBG1 NM_001070 2.16E-11 6.39E-11 -1.153 5.09E-11 -1.17548 topoisomerase (DNA) II alpha 170kDa TOP2A NM_001067 4.18E-08 3.61E-06 -1.15295 4.43E-06 -1.12994 protein phosphatase 4 (formerly X), catalytic subunit PPP4C NM_002720 1.57E-11 1.68E-11 -1.1522 2.77E-11 -1.10397 suppressor of cytokine signaling 1 SOCS1 NM_003745 3.50E-08 3.93E-07 -1.15182 2.91E-08 -1.45881 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatas CTDSPL2 NM_016396 9.38E-08 6.02E-08 -1.15071 3.29E-08 -1.21467 stearoyl-CoA desaturase (delta-9-desaturase) /// stearoyl-CoA desaturase (delta- SCD NM_005063 4.66E-07 2.47E-06 -1.14921 9.72E-07 -1.25621 reticulon 4 interacting protein 1 /// reticulon 4 interacting protein 1 RTN4IP1 NM_032730 3.01E-10 2.57E-10 -1.14901 1.75E-10 -1.18767 CDC23 (cell division cycle 23, yeast, homolog) CDC23 NM_004661 3.94E-10 2.45E-09 -1.14844 5.19E-10 -1.31366 pleiomorphic adenoma gene-like 1 PLAGL1 NM_002656 /// NM_0067181.58E-06 2.00E-06 -1.14767 1.51E-06 -1.17921 of inner mitochondrial membrane 8 homolog A (yeast) TIMM8A NM_004085 1.09E-07 2.53E-08 -1.14726 9.84E-08 -1.01534 solute carrier family 17 (sodium phosphate), member 2 SLC17A2 NM_005835 2.06E-08 9.52E-07 -1.14696 1.28E-07 -1.38074 CAMP-binding guanine nucleotide exchange factor IV (cAMP-GEFIV) mRNA, clone W15, ------2.31E-10 8.50E-11 -1.14684 2.33E-10 -1.05183 barren homolog (Drosophila) BRRN1 NM_015341 1.94E-10 3.87E-10 -1.14644 3.13E-10 -1.16736 cell division cycle 2, G1 to S and G2 to M CDC2 NM_001786 /// NM_0333798.58E-12 1.70E-11 -1.14279 7.78E-11 -1.00403 muscleblind-like 3 (Drosophila) MBNL3 NM_018388 /// NM_1334867.32E-11 4.93E-11 -1.14276 2.49E-11 -1.21146 RAP1 interacting factor homolog (yeast) RIF1 NM_018151 2.83E-06 2.65E-05 -1.14222 2.20E-05 -1.16427 par-6 partitioning defective 6 homolog beta (C. elegans) PARD6B NM_032521 4.33E-10 1.54E-08 -1.14044 2.61E-09 -1.33313 zinc finger protein 200 ZNF200 NM_003454 /// NM_1980879.02E-11 /// NM_1980881.94E-10 -1.14027 2.74E-11 -1.34809 CXXC finger 6 CXXC6 NM_030625 7.22E-11 1.01E-10 -1.14 1.57E-11 -1.3357 gem (nuclear organelle) associated protein 5 GEMIN5 NM_015465 8.58E-16 5.83E-14 -1.13997 1.37E-13 -1.06126 Rab geranylgeranyltransferase, beta subunit RABGGTB NM_004582 5.06E-08 1.73E-08 -1.13971 2.96E-08 -1.08656 mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, iso MGAT4A NM_012214 3.87E-10 4.54E-10 -1.13936 1.79E-10 -1.23433 MAD2 mitotic arrest deficient-like 1 (yeast) MAD2L1 NM_002358 3.10E-08 7.48E-08 -1.13884 9.84E-08 -1.11098 Transcribed locus ------2.60E-07 2.33E-05 -1.13792 2.22E-06 -1.43875 PTX1 protein PTX1 NM_016570 1.53E-08 2.21E-08 -1.13784 3.04E-09 -1.35477 Transcribed locus, strongly similar to XP_421205.1 PREDICTED: similar to thrombo ------7.67E-13 1.81E-09 -1.13604 4.22E-09 -1.05501 chromosome 18 open reading frame 21 C18orf21 NM_031446 1.35E-10 5.00E-11 -1.13589 1.43E-10 -1.03852 HMT1 hnRNP methyltransferase-like 3 (S. cerevisiae) HRMT1L3 NM_005788 5.98E-09 3.85E-09 -1.13585 1.22E-08 -1.02619 TTK protein kinase TTK NM_003318 4.09E-07 6.56E-07 -1.13505 2.80E-07 -1.2286 regulatory solute carrier protein, family 1, member 1 RSC1A1 NM_006511 4.12E-07 1.04E-05 -1.13461 3.16E-06 -1.27692 chromosome X open reading frame 15 CXorf15 NM_018360 2.17E-06 4.68E-06 -1.13182 9.80E-06 -1.05161 GIPC PDZ domain containing family, member 2 GIPC2 NM_017655 1.24E-07 8.88E-08 -1.13124 1.08E-07 -1.11166 exportin 5 XPO5 NM_020750 2.86E-09 1.87E-09 -1.13008 8.52E-10 -1.20962 BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) BUB1 NM_004336 2.61E-08 1.26E-06 -1.1298 6.73E-07 -1.19862 origin recognition complex, subunit 6 homolog-like (yeast) ORC6L NM_014321 8.31E-09 4.08E-08 -1.12969 2.62E-08 -1.17532 ZW10 interactor ZWINT NM_001005413 /// NM_0010054141.39E-13 /// NM_0070571.65E-11 /// NM_032997-1.12848 8.18E-12 -1.19738 cell division cycle associated 1 CDCA1 NM_031423 /// NM_1456971.00E-07 1.43E-06 -1.12826 5.79E-07 -1.22843 hypothetical protein LOC253842 LOC253842 --- 5.09E-10 1.67E-08 -1.12785 4.13E-09 -1.27568 zinc finger protein 689 ZNF689 NM_138447 1.15E-10 6.87E-11 -1.12772 5.71E-11 -1.14566 cyclin B1 CCNB1 NM_031966 1.54E-11 8.73E-11 -1.12728 7.99E-11 -1.13593 similar to RPE-spondin HSUP1 XM_497769 1.63E-07 8.78E-08 -1.12634 2.68E-07 -1.01736 open reading frame 139 C6orf139 NM_018132 8.69E-11 2.73E-06 -1.12629 3.40E-08 -1.69002 cell division cycle associated 3 /// cell division cycle associated 3 CDCA3 NM_031299 4.81E-10 6.53E-09 -1.12547 3.13E-09 -1.20027 polo-like kinase 1 (Drosophila) PLK1 NM_005030 3.60E-11 1.29E-11 -1.12506 4.16E-11 -1.01807 exosome component 8 EXOSC8 NM_181503 1.04E-07 3.68E-07 -1.12244 1.31E-07 -1.23405 phosphatidylinositol glycan, class L PIGL NM_004278 1.51E-10 4.34E-11 -1.12055 1.14E-10 -1.03195 RNA terminal phosphate cyclase domain 1 RTCD1 NM_003729 3.27E-10 1.77E-10 -1.1195 1.11E-10 -1.16537 diacylglycerol O-acyltransferase homolog 1 (mouse) DGAT1 NM_012079 1.27E-08 7.54E-09 -1.11935 6.93E-09 -1.12772 discs, large homolog 7 (Drosophila) DLG7 NM_014750 1.95E-07 1.41E-06 -1.11728 8.90E-07 -1.1672 ATP synthase mitochondrial F1 complex assembly factor 1 ATPAF1 NM_022745 3.61E-10 2.73E-09 -1.11704 1.38E-09 -1.18507 solute carrier family 30, member 10 SLC30A10 NM_001004433 /// NM_0187135.92E-06 2.79E-06 -1.11583 3.01E-06 -1.10791 SMC1 structural maintenance of 1-like 1 (yeast) SMC1L1 NM_006306 3.01E-10 1.73E-10 -1.11565 1.11E-10 -1.15922 vacuolar protein sorting 54 (yeast) VPS54 NM_001005739 /// NM_0165163.41E-11 2.79E-11 -1.11449 9.25E-12 -1.22411 CDC20 cell division cycle 20 homolog (S. cerevisiae) CDC20 NM_001255 1.14E-11 1.80E-10 -1.11286 2.20E-10 -1.09415 methionine-tRNA synthetase 2 (mitochondrial) MARS2 NM_138395 1.51E-08 7.71E-09 -1.11193 5.36E-09 -1.14799 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

CDNA FLJ41107 fis, clone BLADE2007923 ------7.98E-07 1.14E-06 -1.11148 5.69E-07 -1.18661 thymidine kinase 1, soluble TK1 NM_003258 1.83E-08 5.92E-09 -1.11098 1.68E-08 -1.01311 hepatocellular carcinoma-associated gene TD26 LOC55908 NM_018687 2.00E-11 4.08E-09 -1.11015 3.73E-10 -1.36614 GrpE-like 2, mitochondrial (E. coli) GRPEL2 NM_152407 5.45E-09 2.70E-09 -1.1099 2.18E-09 -1.13082 Small nuclear RNA activating complex, polypeptide 5, 19kDa SNAPC5 NM_006049 1.45E-11 5.00E-12 -1.1098 1.72E-11 -0.999647 chromosome 21 open reading frame 45 C21orf45 NM_018944 2.62E-09 2.67E-08 -1.10842 8.45E-08 -0.99934 PTX1 protein PTX1 NM_016570 2.11E-09 1.97E-09 -1.10692 5.98E-10 -1.2275 MCM7 minichromosome maintenance deficient 7 (S. cerevisiae) MCM7 NM_005916 /// NM_1827761.06E-11 6.09E-11 -1.10675 9.35E-11 -1.06689 muted homolog (mouse) MUTED NM_201280 1.13E-09 1.05E-09 -1.10219 2.56E-10 -1.24536 interleukin-1 receptor-associated kinase 2 IRAK2 NM_001570 1.00E-08 1.85E-08 -1.1018 1.12E-08 -1.15235 KIAA1609 protein KIAA1609 NM_020947 2.94E-10 1.94E-09 -1.10135 4.44E-09 -1.02461 KIAA0101 KIAA0101 NM_001029989 /// NM_0147361.29E-11 5.12E-11 -1.09998 3.54E-11 -1.13505 breast cancer 2, early onset BRCA2 NM_000059 1.05E-08 1.48E-07 -1.09996 4.91E-08 -1.21524 F-box protein 5 FBXO5 NM_012177 6.00E-10 3.10E-09 -1.09884 8.87E-09 -1.00217 bromodomain adjacent to zinc finger domain, 1A BAZ1A NM_013448 /// NM_1826482.48E-06 0.00039615 -1.09875 0.00106086 -0.969976 deleted in lymphocytic leukemia, 2 /// BCMS upstream neighbor-like DLEU2 /// BCMSUNLNM_006021 1.38E-07 1.88E-07 -1.09745 2.22E-07 -1.08097 dyskeratosis congenita 1, dyskerin DKC1 NM_001363 7.00E-12 6.56E-10 -1.09726 2.20E-10 -1.20537 chondroitin sulfate proteoglycan 6 (bamacan) CSPG6 NM_005445 8.27E-06 0.000292432 -1.09528 0.00044782 -1.04031 forkhead box K2 FOXK2 NM_004514 /// NM_1814301.32E-07 /// NM_1814311.16E-05 -1.09363 6.53E-06 -1.15872 tyrosyl-DNA phosphodiesterase 1 TDP1 NM_001008744 /// NM_0183191.34E-08 9.97E-09 -1.09313 1.32E-08 -1.06649 DnaJ (Hsp40) homolog, subfamily B, member 9 DNAJB9 NM_012328 1.07E-10 9.04E-09 -1.09253 1.58E-09 -1.27285 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 24 SLC25A24 NM_013386 /// NM_2136512.31E-08 9.07E-09 -1.09156 1.03E-08 -1.07943 carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 9 /// carbohydrate (N- CHST9 NM_031422 1.73E-05 4.32E-05 -1.0905 1.08E-05 -1.25906 pinin, desmosome associated protein PNN NM_002687 5.05E-11 1.42E-10 -1.09032 3.59E-11 -1.22648 Transcribed locus ------1.30E-07 1.95E-07 -1.09005 2.59E-07 -1.06221 polo-like kinase 4 (Drosophila) PLK4 NM_014264 1.08E-10 6.20E-10 -1.08989 8.40E-10 -1.06172 polymerase (DNA directed), alpha POLA NM_016937 5.47E-11 4.20E-11 -1.08874 3.70E-11 -1.1006 DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 DDX18 NM_006773 2.52E-06 4.27E-05 -1.08855 1.86E-05 -1.18758 Erythrocyte membrane protein band 4.1 like 5 EPB41L5 NM_020909 5.66E-10 1.08E-07 -1.08834 1.03E-07 -1.09245 hypothetical protein FLJ20641 FLJ20641 NM_017915 7.43E-08 1.32E-06 -1.08832 1.32E-06 -1.08859 family with sequence similarity 19 (chemokine (C-C motif)-like), member A5 FAM19A5 NM_015381 8.41E-11 1.57E-10 -1.08773 3.04E-10 -1.02758 spastic ataxia of Charlevoix-Saguenay (sacsin) SACS NM_014363 5.84E-08 6.39E-08 -1.08542 3.40E-08 -1.14871 topoisomerase (DNA) II binding protein 1 TOPBP1 NM_007027 1.88E-11 5.36E-11 -1.08503 1.80E-10 -0.978186 SMC2 structural maintenance of chromosomes 2-like 1 (yeast) SMC2L1 NM_006444 9.49E-09 1.05E-07 -1.08501 2.48E-08 -1.23469 ribonucleotide reductase M1 polypeptide RRM1 NM_001033 1.52E-09 3.96E-09 -1.08408 1.62E-09 -1.17215 signal peptidase complex subunit 3 homolog (S. cerevisiae) SPCS3 NM_021928 3.36E-13 1.16E-11 -1.08371 9.12E-12 -1.10637 MRNA; cDNA DKFZp686E16168 (from clone DKFZp686E16168) ------3.68E-10 1.27E-09 -1.08353 4.14E-10 -1.19354 nucleolar and coiled-body phosphoprotein 1 NOLC1 NM_004741 4.75E-15 1.51E-15 -1.08321 2.90E-15 -1.02563 family with sequence similarity 3, member B FAM3B NM_058186 /// NM_2069648.63E-07 9.07E-07 -1.08309 1.56E-06 -1.02908 chromosome 2 open reading frame 3 C2orf3 NM_003203 1.77E-10 2.94E-10 -1.08303 1.49E-10 -1.14777 chromosome 15 open reading frame 23 C15orf23 NM_033286 2.56E-10 1.55E-09 -1.08296 1.61E-09 -1.07962 hypothetical protein FLJ11155 FLJ11155 NM_018342 7.44E-09 2.86E-08 -1.08259 1.12E-08 -1.17606 nucleolar protein 11 NOL11 NM_015462 5.96E-10 1.75E-09 -1.08244 1.20E-09 -1.11846 ATPase, H+ transporting, lysosomal V0 subunit a isoform 2 ATP6V0A2 NM_012463 1.96E-08 3.09E-08 -1.08148 2.94E-08 -1.08644 F-box protein 25 FBXO25 NM_012173 /// NM_1834201.12E-08 /// NM_1834219.98E-09 -1.08011 2.66E-09 -1.21269 hypothetical protein LOC123876 /// acyl-CoA synthetase medium-chain family membe LOC123876 /// ACSM2NM_001010845 /// NM_1826174.45E-09 3.00E-06 -1.07925 7.83E-06 -0.982146 RNA binding protein with multiple splicing RBPMS NM_001008710 /// NM_0010087112.02E-08 /// NM_0010087121.48E-08 /// NM_006867-1.07772 2.12E-08 -1.04398 solute carrier family 7 (cationic amino acid transporter, y+ system), member 6 SLC7A6 NM_003983 6.55E-08 6.72E-08 -1.07698 7.03E-08 -1.07262 hypothetical protein HSPC111 HSPC111 NM_016391 3.70E-11 1.37E-10 -1.07569 1.69E-10 -1.05695 polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa POLR3K NM_016310 1.14E-11 1.44E-11 -1.07538 1.95E-11 -1.04797 CDK5 regulatory subunit associated protein 1-like 1 CDKAL1 NM_017774 7.94E-08 1.04E-06 -1.0745 8.83E-07 -1.09102 exportin 4 XPO4 NM_022459 3.02E-09 1.32E-09 -1.07373 1.88E-09 -1.04156 U2-associated SR140 protein SR140 XM_031553 5.62E-09 2.82E-09 -1.07147 4.35E-09 -1.03175 leucine-rich repeats and immunoglobulin-like domains 3 LRIG3 NM_153377 7.18E-09 1.93E-08 -1.07012 6.25E-09 -1.18209 ras homolog gene family, member B RHOB NM_004040 3.73E-05 4.45E-05 -1.0698 2.61E-05 -1.13182 nucleoporin 160kDa NUP160 NM_015231 1.50E-08 6.27E-09 -1.06885 7.80E-09 -1.04842 nucleoporin 50kDa NUP50 NM_007172 /// NM_1536452.69E-07 /// NM_1536841.39E-06 -1.06747 1.86E-06 -1.03837 rhophilin, Rho GTPase binding protein 2 RHPN2 NM_033103 4.02E-10 7.70E-09 -1.06701 1.68E-08 -0.995866 kinetochore associated 1 KNTC1 NM_014708 2.25E-10 9.21E-10 -1.06695 6.89E-10 -1.09401 nucleolar protein 5A (56kDa with KKE/D repeat) NOL5A NM_006392 1.17E-13 3.10E-13 -1.06656 7.59E-13 -0.989173 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

Ras-GTPase-activating protein SH3-domain-binding protein G3BP NM_005754 /// NM_1983955.11E-12 2.87E-10 -1.06632 1.79E-10 -1.11014 IQ motif containing B1 /// IQ motif containing B1 IQCB1 NM_001023570 /// NM_0010235718.84E-11 5.81E-11 -1.06626 1.52E-10 -0.981899 LSM11, U7 small nuclear RNA associated LSM11 NM_173491 5.62E-09 4.99E-09 -1.06603 1.49E-09 -1.18427 hypothetical protein MGC12916 /// hypothetical protein MGC12916 MGC12916 --- 4.36E-07 1.19E-06 -1.06581 1.16E-07 -1.3234 aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl AASDHPPT NM_015423 6.14E-09 4.17E-09 -1.0656 6.10E-09 -1.03066 Eukaryotic translation initiation factor 2C, 2 EIF2C2 NM_012154 3.47E-09 2.97E-09 -1.0647 8.47E-10 -1.18724 serine palmitoyltransferase, long chain base subunit 2-like (aminotransferase 2) SPTLC2L --- 2.99E-09 1.01E-08 -1.06298 3.98E-09 -1.15353 ATPase, Cu++ transporting, beta polypeptide (Wilson disease) ATP7B NM_000053 /// NM_0010059187.37E-10 1.24E-09 -1.06283 8.73E-10 -1.09592 synovial sarcoma, X breakpoint 2 interacting protein SSX2IP NM_014021 4.70E-08 3.28E-08 -1.0626 1.27E-08 -1.15614 PAK1 interacting protein 1 PAK1IP1 NM_017906 1.19E-10 5.88E-10 -1.0598 3.80E-10 -1.10037 mutS homolog 6 (E. coli) MSH6 NM_000179 2.04E-10 1.83E-10 -1.05896 2.48E-10 -1.03184 Poly(rC) binding protein 2 PCBP2 NM_005016 /// NM_0319898.73E-10 9.82E-10 -1.05878 2.05E-10 -1.21165 U2-associated SR140 protein SR140 XM_031553 1.26E-08 6.71E-09 -1.05871 4.42E-09 -1.0982 KIAA1462 KIAA1462 XM_166132 1.49E-05 7.32E-06 -1.0587 1.36E-05 -0.994644 deleted in liver cancer 1 DLC1 NM_006094 /// NM_0247673.15E-10 /// NM_1826432.49E-10 -1.05693 1.34E-10 -1.11487 apobec-1 complementation factor ACF NM_014576 /// NM_1389321.30E-12 /// NM_1389331.54E-12 -1.05453 5.02E-13 -1.15919 primase, polypeptide 2A, 58kDa PRIM2A NM_000947 6.02E-08 6.13E-07 -1.05441 2.22E-07 -1.15845 hypothetical protein LOC283464 LOC283464 XM_290597 1.16E-10 2.03E-08 -1.0543 9.57E-10 -1.37781 chromosome 10 open reading frame 9 C10orf9 NM_145012 /// NM_1816981.63E-10 6.71E-10 -1.05399 1.45E-09 -0.9858 shugoshin-like 2 (S. pombe) SGOL2 NM_152524 4.64E-07 6.82E-07 -1.05393 3.38E-07 -1.125 DNA2 DNA replication helicase 2-like (yeast) DNA2L XM_166103 2.18E-07 1.23E-07 -1.05298 6.60E-08 -1.11375 tumor suppressor candidate 2 TUSC2 NM_007275 8.37E-12 8.82E-11 -1.05244 6.62E-11 -1.07863 Vacuolar protein sorting 33A (yeast) VPS33A NM_022916 9.46E-06 9.86E-06 -1.05209 9.10E-06 -1.0606 transcription elongation regulator 1 TCERG1 NM_006706 4.69E-08 1.37E-08 -1.05043 3.10E-08 -0.976501 ATP/GTP-binding protein HEAB NM_006831 1.07E-11 9.58E-12 -1.04979 7.94E-12 -1.06664 WD repeat and HMG-box DNA binding protein 1 WDHD1 NM_001008396 /// NM_0070866.78E-08 7.91E-08 -1.04955 7.55E-08 -1.05406 hypothetical protein MAC30 MAC30 NM_014573 1.28E-09 1.36E-09 -1.04951 3.10E-10 -1.19268 Rab geranylgeranyltransferase, beta subunit RABGGTB NM_004582 5.16E-08 4.76E-08 -1.04796 1.24E-08 -1.18163 NIMA (never in mitosis gene a)-related kinase 2 /// NIMA (never in mitosis gene NEK2 NM_002497 4.06E-09 8.49E-09 -1.04731 2.33E-08 -0.957886 chromosome 14 open reading frame 2 C14orf2 NM_004894 1.20E-09 5.25E-10 -1.04617 1.13E-09 -0.978785 nuclear fragile X mental retardation protein interacting protein 1 NUFIP1 NM_012345 2.79E-10 5.04E-09 -1.04369 4.78E-09 -1.04839 vacuolar protein sorting 54 (yeast) VPS54 NM_001005739 /// NM_0165162.06E-09 1.58E-09 -1.04367 6.51E-10 -1.12718 tumor necrosis factor (ligand) superfamily, member 10 /// tumor necrosis factor TNFSF10 NM_003810 1.38E-06 5.67E-06 -1.04181 5.21E-07 -1.30938 Fad1, flavin adenine dinucleotide synthetase, homolog (yeast) FLAD1 NM_025207 /// NM_2013981.24E-08 5.02E-09 -1.04092 5.50E-09 -1.03264 hypothetical protein LOC158563 LOC158563 --- 9.67E-12 8.56E-10 -1.04042 2.67E-10 -1.15022 cAMP responsive element binding protein 3-like 2 CREB3L2 NM_194071 4.78E-12 1.28E-10 -1.03962 7.41E-11 -1.08954 FIP1 like 1 (S. cerevisiae) FIP1L1 NM_030917 6.72E-08 3.17E-06 -1.03752 4.71E-07 -1.24356 glycogenin 2 GYG2 NM_003918 1.29E-06 4.49E-07 -1.03703 8.61E-07 -0.975819 Chromosome 6 open reading frame 160 chromosome 6 openXM_499041 reading frame 160 1.26E-05 1.34E-05 -1.03688 9.74E-06 -1.07056 replication factor C (activator 1) 2, 40kDa RFC2 NM_002914 /// NM_1814713.88E-11 1.67E-10 -1.0366 1.68E-10 -1.03604 chromosome 1 open reading frame 135 C1orf135 NM_024037 1.48E-07 8.64E-08 -1.03512 7.13E-08 -1.05323 carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 9 CHST9 NM_031422 1.70E-06 1.90E-06 -1.03471 1.54E-06 -1.0557 meiosis-specific nuclear structural 1 MNS1 NM_018365 1.50E-05 9.52E-06 -1.03467 6.06E-06 -1.08242 chromosome 13 open reading frame 1 C13orf1 NM_020456 1.95E-08 3.35E-07 -1.03433 3.26E-07 -1.0371 chromosome 1 open reading frame 25 C1orf25 NM_030934 1.62E-09 1.05E-08 -1.03421 1.36E-08 -1.01071 syntaxin 3A STX3A NM_004177 4.89E-10 4.59E-09 -1.03382 3.23E-09 -1.06627 hypothetical protein MAC30 MAC30 NM_014573 2.64E-09 2.14E-09 -1.03324 7.71E-10 -1.12907 chromosome 1 open reading frame 131 C1orf131 NM_152379 3.50E-13 4.17E-11 -1.03208 3.27E-11 -1.05369 chromosome 20 open reading frame 112 C20orf112 NM_080616 1.89E-12 6.74E-11 -1.03129 5.49E-11 -1.04943 proteasome (prosome, macropain) activator subunit 4 PSME4 NM_014614 6.06E-12 2.58E-11 -1.02839 1.05E-11 -1.10994 BRCA2 and CDKN1A interacting protein BCCIP NM_016567 /// NM_0784683.28E-07 /// NM_0784691.83E-07 -1.02831 4.10E-07 -0.954844 immunoglobulin superfamily, member 1 IGSF1 NM_001555 /// NM_2058334.54E-11 5.43E-09 -1.02794 5.59E-10 -1.2527 erythrocyte membrane protein band 4.1 like 5 EPB41L5 NM_020909 2.83E-12 3.18E-08 -1.02663 2.51E-08 -1.04853 family with sequence similarity 29, member A FAM29A NM_017645 1.44E-09 3.74E-08 -1.02646 9.77E-09 -1.15644 zuotin related factor 1 ZRF1 XM_168590 /// XM_3799094.18E-09 5.46E-09 -1.02611 1.22E-08 -0.955685 IMP4, U3 small nucleolar ribonucleoprotein, homolog (yeast) IMP4 NM_033416 5.25E-10 3.46E-10 -1.02558 5.18E-10 -0.990505 ribonucleotide reductase M1 polypeptide RRM1 NM_001033 4.49E-09 7.81E-09 -1.02532 8.38E-09 -1.01897 polymerase (RNA) I associated factor 1 PRAF1 NM_022490 1.12E-10 1.81E-10 -1.02517 3.60E-10 -0.966585 kinesin family member 23 KIF23 NM_004856 /// NM_1385552.92E-08 1.54E-07 -1.02481 5.28E-08 -1.12912 organic solute transporter beta OSTbeta NM_178859 8.12E-09 1.23E-08 -1.02405 2.26E-09 -1.18841 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

RNA binding protein with multiple splicing RBPMS NM_001008710 /// NM_0010087111.81E-09 /// NM_0010087121.57E-09 /// NM_006867-1.02064 2.71E-09 -0.973358 cyclin E2 CCNE2 NM_057735 /// NM_0577491.19E-09 1.02E-08 -1.02008 1.68E-09 -1.19449 chromosome 9 open reading frame 76 C9orf76 NM_024945 6.82E-09 2.47E-09 -1.01962 3.60E-09 -0.986764 basic transcription factor 3-like 4 BTF3L4 NM_152265 7.65E-09 1.83E-08 -1.01952 5.36E-09 -1.13612 Importin 7 IPO7 NM_006391 4.00E-10 3.83E-10 -1.0184 1.93E-10 -1.08039 thymopoietin TMPO NM_001032283 /// NM_0010322845.46E-10 /// NM_0032765.21E-07 -1.01821 4.16E-07 -1.03992 hypothetical protein MGC12916 /// hypothetical protein MGC12916 MGC12916 --- 1.20E-08 6.58E-09 -1.01567 5.55E-09 -1.03092 mitochondrial fission regulator 1 MTFR1 NM_014637 6.72E-10 7.38E-10 -1.01541 3.55E-10 -1.08166 ------5.13E-07 3.66E-06 -1.01538 7.75E-07 -1.17788 DEAD (Asp-Glu-Ala-As) box polypeptide 19A DDX19A NM_018332 4.12E-06 1.68E-06 -1.01516 2.00E-06 -0.998752 solute carrier family 35, member D2 SLC35D2 NM_007001 6.38E-11 3.42E-11 -1.01496 2.26E-11 -1.05144 zinc finger protein 267 ZNF267 NM_003414 9.14E-10 6.45E-08 -1.01487 2.24E-08 -1.11578 haptoglobin-related protein HPR NM_020995 2.52E-05 0.000284026 -1.01385 8.55E-05 -1.16306 guanosine monophosphate reductase 2 GMPR2 NM_001002000 /// NM_0010020014.52E-11 /// NM_0010020021.54E-10 /// NM_016576-1.01324 1.97E-10 -0.99213 stearoyl-CoA desaturase (delta-9-desaturase) SCD NM_005063 1.68E-08 3.76E-07 -1.01303 3.96E-08 -1.2433 AER61 glycosyltransferase AER61 NM_173654 3.09E-06 3.94E-06 -1.0121 5.79E-07 -1.21537 Thymopoietin TMPO NM_001032283 /// NM_0010322841.74E-11 /// NM_0032765.03E-10 -1.0118 1.45E-10 -1.12581 zinc finger, CCHC domain containing 10 ZCCHC10 NM_017665 1.41E-07 9.01E-07 -1.01167 3.31E-07 -1.11068 multidrug resistance-related protein /// multidrug resistance-related protein MGC13170 NM_199249 /// NM_1992502.34E-09 7.42E-09 -1.01031 9.98E-09 -0.984266 dyskeratosis congenita 1, dyskerin DKC1 NM_001363 2.13E-16 4.49E-14 -1.00962 2.31E-14 -1.06746 chromosome 10 open reading frame 6 C10orf6 NM_018121 4.41E-11 2.08E-09 -1.00957 7.34E-10 -1.10493 CSE1 chromosome segregation 1-like (yeast) CSE1L NM_001316 /// NM_1774365.04E-08 2.69E-08 -1.00839 4.85E-08 -0.956697 Solute carrier family 4, sodium bicarbonate cotransporter, member 5 SLC4A5 NM_021196 /// NM_0333231.26E-09 /// NM_1334789.94E-10 /// NM_133479 -1.00803 1.76E-09 -0.959087 transforming, acidic coiled-coil containing protein 3 TACC3 NM_006342 3.43E-10 2.91E-09 -1.00643 1.06E-09 -1.09929 Transcribed locus ------1.48E-05 1.19E-05 -1.00552 3.17E-06 -1.14611 UDP-glucose ceramide glucosyltransferase UGCG NM_003358 3.62E-10 3.23E-10 -1.00549 2.36E-10 -1.03289 hypothetical protein MAC30 MAC30 NM_014573 3.62E-09 5.14E-09 -1.00548 8.08E-10 -1.18118 TPX2, microtubule-associated, homolog (Xenopus laevis) TPX2 NM_012112 7.14E-11 7.26E-11 -1.00527 5.14E-11 -1.0353 survival of motor neuron protein interacting protein 1 SIP1 NM_001009182 /// NM_0010091837.87E-07 /// NM_0036162.89E-06 -1.00512 3.41E-07 -1.23036 Transcription factor Dp-1 TFDP1 NM_007111 1.10E-09 8.53E-08 -1.00341 2.18E-08 -1.13376 Transcribed locus ------5.22E-07 1.19E-06 -1.00335 7.35E-08 -1.29784 dimethyladenosine transferase HSA9761 NM_014473 1.09E-09 7.11E-08 -1.00315 1.06E-07 -0.967448 putatative 28 kDa protein LOC56902 NM_020143 8.79E-08 4.37E-08 -1.00255 3.83E-08 -1.01448 ring finger and FYVE-like domain containing 1 RFFL NM_001017368 /// NM_0571787.59E-10 2.01E-08 -1.00154 4.27E-09 -1.14789 stress-associated endoplasmic reticulum protein 1 SERP1 NM_014445 6.33E-12 2.12E-09 -1.00114 3.71E-09 -0.953536 small optic lobes homolog (Drosophila) SOLH NM_005632 5.80E-06 1.90E-05 -1.00076 2.84E-06 -1.20986 peptidyl prolyl isomerase H (cyclophilin H) PPIH NM_006347 2.10E-09 2.59E-09 -0.999693 1.38E-09 -1.05611 KIAA1333 KIAA1333 NM_017769 2.21E-07 1.53E-06 -0.999281 1.93E-06 -0.977586 eukaryotic translation initiation factor 4E EIF4E NM_001968 1.78E-05 1.41E-05 -0.999066 6.16E-06 -1.08553 transforming growth factor beta regulator 4 TBRG4 NM_004749 /// NM_0309001.77E-10 /// NM_1991224.24E-10 -0.99826 2.85E-10 -1.03318 UDP-glucose ceramide glucosyltransferase UGCG NM_003358 1.11E-07 2.13E-07 -0.99759 8.22E-08 -1.08806 activin A receptor, type IB ACVR1B NM_004302 /// NM_0203273.49E-09 /// NM_0203281.74E-09 -0.997157 1.31E-09 -1.02195 chromosome 20 open reading frame 129 C20orf129 NM_030919 8.12E-10 7.70E-10 -0.996593 1.13E-09 -0.964011 TDP-glucose 4,6-dehydratase TGDS NM_014305 8.58E-12 1.61E-09 -0.996511 1.25E-09 -1.01863 nucleoporin 37kDa NUP37 NM_024057 1.06E-10 2.06E-09 -0.994959 7.29E-10 -1.08893 alkylglycerone phosphate synthase AGPS NM_003659 8.23E-10 1.03E-09 -0.992589 3.77E-10 -1.08273 chromosome 14 open reading frame 94 C14orf94 NM_017815 7.40E-08 2.91E-08 -0.992099 3.21E-08 -0.983391 zinc finger protein 689 ZNF689 NM_138447 1.25E-08 6.08E-09 -0.991887 8.13E-09 -0.96685 ------9.32E-08 4.84E-06 -0.990035 4.60E-07 -1.2396 GTP binding protein 4 GTPBP4 NM_012341 2.68E-11 1.98E-10 -0.989627 1.59E-10 -1.00824 DPH5 homolog (S. cerevisiae) DPH5 NM_015958 4.83E-07 2.72E-07 -0.988319 1.86E-07 -1.02367 nucleoporin 205kDa NUP205 NM_015135 5.13E-10 2.65E-10 -0.986742 1.80E-10 -1.01995 ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F ATP6V1F NM_004231 2.60E-11 6.24E-10 -0.986324 7.21E-10 -0.974186 phosphatidylinositol glycan, class C PIGC NM_002642 /// NM_1537473.80E-09 2.50E-09 -0.984912 1.98E-09 -1.00505 coilin COIL NM_004645 1.38E-09 5.65E-10 -0.984782 7.37E-10 -0.96252 CDNA FLJ11381 fis, clone HEMBA1000501 ------3.07E-06 2.33E-06 -0.984496 2.35E-06 -0.984049 cyclin F CCNF NM_001761 3.79E-06 2.83E-06 -0.984294 8.82E-07 -1.10014 ribosomal protein S6 kinase, 90kDa, polypeptide 3 RPS6KA3 NM_004586 1.33E-09 3.22E-09 -0.984289 3.61E-09 -0.974475 hypothetical protein FLJ11155 FLJ11155 NM_018342 3.11E-06 4.77E-06 -0.982607 7.74E-07 -1.17003 BRCA1 interacting protein C-terminal helicase 1 BRIP1 NM_032043 4.59E-08 2.34E-08 -0.981947 2.96E-08 -0.961514 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

cyclin-dependent kinase 8 CDK8 NM_001260 2.51E-08 3.05E-07 -0.980928 1.28E-07 -1.06167 DnaJ (Hsp40) homolog, subfamily A, member 2 DNAJA2 NM_005880 3.69E-10 2.70E-09 -0.980541 3.45E-09 -0.959674 Proline rich membrane anchor 1 PRIMA1 NM_178004 /// NM_1780132.89E-08 3.78E-07 -0.980522 2.06E-07 -1.03688 antigen identified by monoclonal antibody Ki-67 MKI67 NM_002417 1.24E-12 1.47E-10 -0.980183 3.61E-11 -1.1049 survival of motor neuron protein interacting protein 1 SIP1 NM_001009182 /// NM_0010091832.58E-08 /// NM_0036164.99E-08 -0.979699 6.62E-09 -1.17213 integrin beta 3 binding protein (beta3-endonexin) ITGB3BP NM_014288 1.60E-06 7.33E-06 -0.979114 2.28E-06 -1.09764 liver-expressed antimicrobial peptide 2 LEAP-2 NM_052971 6.13E-07 7.30E-07 -0.978089 3.57E-07 -1.04553 GrpE-like 2, mitochondrial (E. coli) GRPEL2 NM_152407 0.000153736 0.000122243 -0.977603 0.000113679 -0.985615 coilin COIL NM_004645 1.22E-06 5.60E-06 -0.977247 3.53E-06 -1.0226 Transcribed locus ------4.85E-07 1.69E-06 -0.976666 9.74E-07 -1.02946 primase, polypeptide 2A, 58kDa PRIM2A NM_000947 5.38E-08 9.10E-08 -0.976658 1.13E-07 -0.957865 GrpE-like 1, mitochondrial (E. coli) GRPEL1 NM_025196 6.36E-13 6.85E-13 -0.975226 6.85E-13 -0.975274 coiled-coil domain containing 5 (spindle associated) CCDC5 NM_138443 1.27E-06 7.80E-07 -0.973829 4.74E-07 -1.02023 CGI-115 protein CGI-115 NM_016052 5.48E-08 3.31E-08 -0.973611 3.01E-08 -0.981967 importin 4 IPO4 NM_024658 5.86E-09 6.76E-07 -0.973463 7.50E-07 -0.96403 Fas (TNF receptor superfamily, member 6) FAS NM_000043 /// NM_1528715.58E-10 /// NM_1528725.45E-07 /// NM_152873 -0.970827/// NM_152874 /// 5.19E-07NM_152875 -0.975378 KIAA1212 KIAA1212 NM_018084 1.02E-08 4.31E-07 -0.969959 2.41E-07 -1.02344 thymopoietin TMPO NM_001032283 /// NM_0010322846.67E-08 /// NM_0032761.29E-06 -0.967925 6.60E-07 -1.0313 chromosome 1 open reading frame 55 C1orf55 NM_152608 1.70E-11 4.01E-09 -0.966865 8.52E-10 -1.10641 transcription factor A, mitochondrial TFAM NM_003201 4.71E-09 6.12E-09 -0.966103 3.69E-09 -1.00987 CCAAT/enhancer binding protein (C/EBP), delta CEBPD NM_005195 1.94E-11 8.41E-08 -0.965608 6.10E-08 -0.993949 hypothetical protein LOC158563 LOC158563 --- 5.79E-08 8.50E-07 -0.96429 3.10E-07 -1.0593 Chromosome 18 open reading frame 50 chromosome 18 open--- reading frame 50 1.93E-09 3.01E-08 -0.964103 1.98E-08 -1.00058 antigen identified by monoclonal antibody Ki-67 MKI67 NM_002417 2.89E-10 1.09E-09 -0.963646 4.96E-10 -1.03182 RRN3 RNA polymerase I transcription factor homolog (yeast) RRN3 NM_018427 7.10E-06 0.0105438 -0.963467 0.00755462 -1.02061 kinesin family member 2C KIF2C NM_006845 6.48E-11 2.44E-10 -0.962309 2.72E-10 -0.953349 vesicle-associated membrane protein 3 (cellubrevin) VAMP3 NM_004781 7.13E-16 7.84E-10 -0.962224 1.34E-10 -1.12043 phosphate cytidylyltransferase 1, choline, beta PCYT1B NM_004845 4.83E-07 4.18E-07 -0.961853 3.54E-07 -0.976765 dyskeratosis congenita 1, dyskerin DKC1 NM_001363 4.82E-12 7.28E-10 -0.961674 3.56E-10 -1.02294 CHK1 checkpoint homolog (S. pombe) CHEK1 NM_001274 3.79E-10 3.76E-08 -0.960186 3.19E-08 -0.974288 vesicle-associated membrane protein 3 (cellubrevin) VAMP3 NM_004781 7.16E-19 1.81E-13 -0.959299 8.28E-14 -1.02436 hypothetical protein LOC283377 LOC283377 NM_207344 4.77E-09 4.69E-08 -0.95919 1.71E-08 -1.04947 kinesin family member 18A /// kinesin family member 18A KIF18A NM_031217 6.11E-08 2.52E-07 -0.956975 9.78E-08 -1.04342 IGF-II mRNA-binding protein 3 IMP-3 NM_006547 3.63E-09 3.20E-09 -0.956942 2.40E-09 -0.981308 chromosome 13 open reading frame 7 C13orf7 NM_024546 2.19E-07 3.69E-06 -0.953688 1.25E-06 -1.05816 kinesin family member 2C KIF2C NM_006845 6.38E-08 2.77E-07 -0.95333 1.48E-07 -1.00993 transcription factor A, mitochondrial TFAM NM_003201 1.57E-09 2.03E-09 -0.952683 1.46E-09 -0.98022 Hypothetical protein MGC13102 MGC13102 NM_032323 2.62E-07 1.65E-06 0.950769 2.00E-07 1.15827 contactin associated protein-like 3 /// contactin associated protein-like 3B CNTNAP3 /// CNTNAP3BNM_033655 /// XM_3720971.39E-05 1.22E-05 0.952639 1.24E-05 0.950855 histone 2, H2aa HIST2H2AA NM_003516 1.97E-11 4.27E-07 0.953031 1.71E-07 1.03718 transmembrane protein with EGF-like and two follistatin-like domains 1 TMEFF1 NM_003692 4.90E-06 4.18E-06 0.954697 1.15E-06 1.08118 CDNA FLJ31683 fis, clone NT2RI2005353 ------5.94E-08 3.85E-07 0.955598 1.25E-07 1.05931 similar to Interferon-induced transmembrane protein 3 (Interferon-inducible prot LOC391020 XM_497663 8.56E-10 6.18E-09 0.955863 1.98E-09 1.05594 transgelin TAGLN NM_001001522 /// NM_0031864.90E-13 2.95E-08 0.956428 7.18E-10 1.32458 integrin, beta 5 ITGB5 NM_002213 1.32E-10 1.38E-10 0.957778 5.46E-11 1.03663 Nuclear receptor subfamily 2, group F, member 1 NR2F1 NM_005654 2.35E-09 8.10E-09 0.959268 6.20E-10 1.19991 hypothetical protein LOC283852 LOC283852 --- 2.24E-10 5.54E-10 0.959935 9.19E-11 1.11995 methionine sulfoxide reductase B3 MSRB3 NM_001031679 /// NM_1980801.78E-06 1.30E-06 0.961486 5.25E-07 1.04677 Homo sapiens, Similar to neuronal thread protein, clone IMAGE:4102657, mRNA ------1.64E-07 1.92E-07 0.961593 3.11E-08 1.1332 TNF receptor-associated factor 5 TRAF5 NM_001033910 /// NM_0046194.59E-05 /// NM_1457590.000109403 0.964539 9.84E-06 1.24426 H2A histone family, member Y H2AFY NM_004893 /// NM_1386093.05E-08 /// NM_1386101.18E-07 0.967758 2.97E-08 1.09607 plexin B2 PLXNB2 XM_371474 1.25E-07 1.15E-07 0.970704 5.65E-08 1.03494 tetratricopeptide repeat domain 3 TTC3 NM_001001894 /// NM_0033161.15E-08 4.20E-06 0.970738 2.12E-06 1.0374 ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) UBE2H NM_003344 /// NM_1826977.78E-08 9.24E-08 0.972697 7.88E-08 0.986785 chondroitin sulfate GalNAcT-2 GALNACT-2 NM_018590 6.43E-09 7.70E-09 0.974377 2.24E-09 1.0854 SRY (sex determining region Y)-box 4 SOX4 NM_003107 8.65E-07 5.12E-07 0.97619 3.42E-07 1.01345 CDNA clone IMAGE:5276760 ------0.000487614 0.000309904 0.978253 0.000135578 1.07693 Kruppel-like factor 5 (intestinal) KLF5 NM_001730 5.85E-07 4.37E-07 0.978637 4.94E-07 0.96753 limb region 1 homolog (mouse) LMBR1 NM_022458 2.84E-07 4.91E-07 0.979921 1.79E-07 1.07529 zinc finger protein 706 ZNF706 NM_016096 1.55E-06 1.65E-06 0.980015 5.59E-07 1.08563 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

septin 6 7-Sep NM_015129 /// NM_1457991.86E-13 /// NM_1458001.96E-12 /// NM_145802 0.980736 1.52E-12 1.00194 PTEN induced putative kinase 1 PINK1 NM_032409 1.16E-08 5.42E-08 0.98077 2.22E-08 1.0624 Similar to 60S ribosomal protein L35 RPL35 NM_007209 1.45E-11 8.03E-12 0.98196 4.92E-12 1.02365 iduronate 2-sulfatase (Hunter syndrome) IDS NM_000202 /// NM_0061233.09E-08 3.08E-07 0.982589 1.95E-07 1.0247 protein tyrosine phosphatase, receptor type, N polypeptide 2 PTPRN2 NM_002847 /// NM_1308421.15E-08 /// NM_1308431.06E-07 0.982667 1.07E-08 1.20577 hypothetical protein DKFZp434N035 DKFZp434N035 NM_032262 3.51E-07 4.52E-07 0.984592 6.46E-08 1.1764 met proto-oncogene (hepatocyte growth factor receptor) MET NM_000245 1.58E-10 1.13E-10 0.985526 8.62E-11 1.00876 septin 6 /// cytokine-like nuclear factor n-pac SEPT6 /// N-PAC NM_015129 /// NM_0325693.78E-11 /// NM_1457995.59E-09 /// NM_145800 ///0.98766 NM_145802 2.52E-10 1.29201 transmembrane protein 76 TMEM76 XM_372038 1.15E-09 2.37E-09 0.98798 3.39E-09 0.957731 zinc finger protein 161 ZNF161 NM_007146 9.12E-09 2.17E-07 0.989493 1.03E-08 1.30172 heparan sulfate 2-O-sulfotransferase 1 HS2ST1 NM_012262 4.96E-06 2.04E-05 0.990239 4.92E-06 1.14314 zinc finger protein 90 homolog (mouse) ZFP90 NM_133458 2.52E-09 1.17E-08 0.990245 5.35E-09 1.06089 eEF1A2 binding protein DKFZp434B1231 NM_178275 2.99E-05 5.29E-05 0.990668 2.80E-05 1.05974 platelet-derived growth factor alpha polypeptide PDGFA NM_002607 /// NM_0330239.72E-06 8.10E-05 0.990702 9.05E-05 0.978697 solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 SLC7A2 NM_001008539 /// NM_0030462.20E-09 4.38E-09 0.991347 5.39E-10 1.18931 SH3 domain binding glutamic acid-rich protein like SH3BGRL NM_003022 9.80E-08 1.11E-07 0.991428 9.86E-08 1.0024 hypothetical protein LOC161527 LOC161527 XM_496088 3.76E-09 1.94E-08 0.994667 2.56E-08 0.970169 hypothetical protein FLJ20719 FLJ20719 XM_373827 /// XM_4984274.54E-08 5.02E-08 0.995713 2.39E-08 1.06398 2',5'-oligoadenylate synthetase 1, 40/46kDa OAS1 NM_001032409 /// NM_0025347.89E-09 /// NM_0168168.42E-09 0.996278 1.03E-08 0.9788 tetratricopeptide repeat domain 3 TTC3 NM_001001894 /// NM_0033163.73E-13 2.02E-11 0.996787 9.66E-12 1.06102 platelet derived growth factor C PDGFC NM_016205 4.41E-09 2.78E-09 0.997693 1.37E-09 1.06108 insulin-like growth factor binding protein 7 IGFBP7 NM_001553 1.57E-05 3.47E-05 0.997886 2.59E-06 1.29619 protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 1 PCMTD1 NM_052937 1.14E-10 9.36E-10 0.999071 1.48E-09 0.959949 SH3-domain GRB2-like endophilin B1 SH3GLB1 NM_016009 6.76E-13 4.47E-11 1.00377 1.63E-12 1.32937 neurobeachin NBEA NM_015678 4.80E-10 1.29E-06 1.00482 9.33E-07 1.03578 hypothetical protein LOC147670 LOC147670 XM_097265 2.58E-06 5.77E-05 1.00564 6.98E-05 0.985133 hypothetical protein FLJ11259 FLJ11259 NM_018370 5.12E-09 3.97E-09 1.00586 1.36E-09 1.10425 deafness, autosomal dominant 5 DFNA5 NM_004403 3.85E-12 4.01E-11 1.00597 2.79E-11 1.03752 topoisomerase (DNA) I, mitochondrial TOP1MT NM_052963 1.31E-09 1.38E-08 1.00604 2.19E-10 1.4429 KIAA1199 KIAA1199 NM_018689 7.30E-09 1.35E-07 1.00638 2.23E-07 0.961118 Ysg2 homolog (mouse) /// Ysg2 homolog (mouse) YSG2 NM_170601 7.36E-07 4.04E-07 1.00702 6.45E-07 0.964194 myosin VI MYO6 NM_004999 1.52E-07 7.85E-08 1.00757 5.04E-08 1.04856 transforming growth factor, beta 1 (Camurati-Engelmann disease) TGFB1 NM_000660 8.70E-09 3.59E-08 1.00807 9.89E-09 1.13028 forkhead box O3A FOXO3A NM_001455 /// NM_2015592.69E-08 2.69E-08 1.00881 7.98E-09 1.1234 T-box 3 (ulnar mammary syndrome) TBX3 NM_005996 /// NM_0165691.64E-10 8.41E-09 1.00932 8.26E-10 1.23563 human immunodeficiency virus type I enhancer binding protein 2 HIVEP2 NM_006734 7.09E-10 7.94E-09 1.00989 3.36E-09 1.08905 scavenger receptor class B, member 2 SCARB2 NM_005506 6.68E-14 4.64E-13 1.01023 8.54E-13 0.959545 CD7 antigen (p41) CD7 NM_006137 2.06E-09 8.85E-08 1.01117 5.81E-08 1.05038 calpain 2, (m/II) large subunit CAPN2 NM_001748 3.33E-18 5.84E-14 1.01137 1.86E-14 1.11312 absent in melanoma 1-like AIM1L NM_017977 9.54E-08 5.93E-07 1.01154 9.83E-07 0.964636 trinucleotide repeat containing 6A TNRC6A NM_014494 /// NM_0208472.19E-11 2.64E-10 1.01335 4.49E-10 0.968328 TSC22 domain family, member 1 TSC22D1 NM_006022 /// NM_1834223.11E-13 4.02E-12 1.01348 2.02E-12 1.07436 annexin A3 ANXA3 NM_005139 2.79E-09 3.53E-09 1.01442 5.49E-10 1.19238 metallothionein 2A MT2A NM_005953 9.09E-11 7.81E-11 1.01463 4.39E-11 1.06562 nuclear receptor binding protein 2 NRBP2 NM_178564 3.89E-09 1.05E-08 1.01502 1.79E-09 1.18497 kinesin 2 KNS2 NM_005552 /// NM_1829236.31E-10 1.86E-09 1.01705 1.39E-09 1.04334 hypothetical protein FLJ11273 FLJ11273 NM_018374 2.04E-07 1.15E-07 1.02001 6.24E-08 1.07811 integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) ITGA3 NM_002204 /// NM_0055010.00108914 0.000631317 1.02065 0.000248871 1.14308 open reading frame 23 C3orf23 NM_001029839 /// NM_0010298409.05E-10 /// NM_1738261.40E-09 1.02295 5.92E-10 1.10208 Ral GEF with PH domain and SH3 binding motif 2 RALGPS2 NM_018037 /// NM_1526633.20E-08 1.79E-08 1.02554 2.54E-08 0.993937 clathrin, light polypeptide (Lcb) /// clathrin, light polypeptide (Lcb) CLTB NM_001834 /// NM_0070971.05E-10 7.43E-11 1.02555 3.05E-11 1.10639 Membrane associated DNA binding protein MNAB NM_018835 1.91E-11 3.77E-09 1.02596 2.23E-09 1.07416 hypothetical protein LOC340061 LOC340061 NM_198282 2.03E-08 1.66E-08 1.02608 8.52E-09 1.08839 chromosome 10 open reading frame 11 C10orf11 NM_032024 5.10E-10 5.41E-09 1.02664 2.95E-09 1.08278 complement component 2 C2 NM_000063 4.04E-09 2.80E-06 1.02721 1.31E-06 1.10523 vesicle-associated membrane protein 1 (synaptobrevin 1) VAMP1 NM_014231 /// NM_0168301.89E-07 /// NM_1992452.00E-07 1.02772 9.21E-08 1.10311 activated leukocyte cell adhesion molecule ALCAM NM_001627 6.06E-09 1.02E-07 1.02777 8.03E-09 1.28967 acylphosphatase 2, muscle type ACYP2 NM_138448 3.08E-10 2.02E-10 1.03172 3.17E-10 0.992602 deoxyribonuclease II, lysosomal DNASE2 NM_001375 4.65E-09 5.69E-09 1.03265 9.06E-10 1.21196 chromosome 20 open reading frame 19 C20orf19 NM_018474 4.93E-09 6.28E-09 1.03272 5.53E-09 1.04432 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

PBX/knotted 1 homeobox 1 PKNOX1 NM_004571 /// NM_1979761.04E-05 4.61E-06 1.03599 3.65E-06 1.05989 SH3-domain binding protein 5 (BTK-associated) SH3BP5 NM_001018009 /// NM_0048441.00E-07 1.21E-07 1.03609 7.36E-08 1.0834 Chromosome 2 open reading frame 17 C2orf17 NM_024293 5.05E-06 1.92E-06 1.0366 2.50E-06 1.01073 WNK deficient protein kinase 4 WNK4 NM_032387 1.34E-06 9.05E-07 1.03681 8.20E-07 1.0464 matrix-remodelling associated 7 MXRA7 NM_001008528 /// NM_0010085291.84E-12 /// NM_1985302.65E-11 1.03791 6.29E-12 1.17259 filamin binding LIM protein 1 FBLIM1 NM_001024215 /// NM_0010242163.03E-09 /// NM_0175561.08E-08 1.03967 8.82E-09 1.05815 FLJ43339 protein FLJ43339 NM_207380 5.22E-08 1.87E-07 1.04038 2.01E-08 1.27179 chromosome 16 open reading frame 45 C16orf45 NM_033201 3.97E-11 1.25E-09 1.04402 7.00E-10 1.09776 Homo sapiens, Similar to LOC169932, clone IMAGE:4499203, mRNA ------2.87E-08 2.53E-07 1.04415 5.24E-07 0.976123 inositol hexaphosphate kinase 2 IHPK2 NM_001005909 /// NM_0010059104.02E-12 /// NM_0010059111.91E-11 /// NM_0010059121.04529 /// NM_0010059135.88E-12 1.15483 protein tyrosine phosphatase type IVA, member 1 PTP4A1 NM_003463 7.09E-13 5.16E-11 1.04734 4.05E-12 1.29991 biliverdin reductase A BLVRA NM_000712 6.47E-09 2.44E-09 1.0483 3.00E-09 1.02978 arylacetamide deacetylase-like 1 AADACL1 NM_020792 9.21E-09 6.80E-09 1.04862 2.71E-09 1.13663 metallothionein 1H MT1H NM_005951 3.59E-10 1.81E-10 1.0508 1.35E-10 1.07748 glutaminase GLS NM_014905 1.72E-06 2.59E-06 1.05083 4.98E-06 0.986097 LOC440461 --- XM_498680 2.44E-06 3.79E-05 1.05096 4.72E-06 1.29944 serine/threonine kinase 17a (apoptosis-inducing) STK17A NM_004760 1.47E-11 1.48E-11 1.05205 1.34E-11 1.06093 secretory carrier membrane protein 1 SCAMP1 NM_004866 /// NM_0528222.19E-09 5.29E-08 1.05359 8.31E-08 1.01163 ring finger protein 144 RNF144 NM_014746 3.60E-11 5.80E-10 1.05483 7.09E-11 1.26332 metallothionein 1E (functional) MT1E NM_175617 5.17E-10 3.51E-10 1.05591 6.84E-10 0.996749 cell cycle progression 1 CCPG1 NM_004748 /// NM_0207395.81E-07 3.44E-07 1.05609 3.10E-07 1.06636 glucuronyl C5-epimerase GLCE NM_015554 9.04E-10 2.72E-09 1.0572 1.48E-09 1.11462 transient receptor potential cation channel, subfamily V, member 2 TRPV2 NM_016113 2.58E-05 0.000167077 1.05912 0.000132815 1.08718 disrupted in schizophrenia 1 DISC1 NM_001012957 /// NM_0010129581.05E-11 /// NM_0010129595.81E-09 /// NM_0186621.0592 5.78E-10 1.29466 STEAP family member 3 STEAP3 NM_001008410 /// NM_0182346.59E-12 /// NM_1829152.97E-09 1.05983 1.59E-10 1.36539 clathrin, light polypeptide (Lcb) CLTB NM_001834 /// NM_0070972.47E-09 2.60E-09 1.05986 6.28E-10 1.19869 alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevi ATRX NM_000489 /// NM_1382701.18E-09 /// NM_1382715.47E-08 1.06308 1.13E-08 1.2236 Similar to lymphocyte-specific protein 1 --- XM_496572 1.06E-12 1.85E-12 1.06502 8.00E-13 1.14321 MAX interactor 1 /// MAX interactor 1 MXI1 NM_001008541 /// NM_0059624.83E-13 /// NM_1304392.81E-12 1.06582 6.78E-13 1.20182 matrix metallopeptidase 7 (matrilysin, uterine) MMP7 NM_002423 0.000136966 4.71E-05 1.06629 5.59E-05 1.0468 interferon induced transmembrane protein 2 (1-8D) IFITM2 NM_006435 6.00E-10 3.11E-09 1.06697 6.49E-10 1.22251 KIAA1223 protein KIAA1223 NM_020337 6.77E-09 4.45E-09 1.06803 4.36E-09 1.0699 tribbles homolog 2 (Drosophila) TRIB2 NM_021643 6.94E-11 7.05E-08 1.06996 5.11E-08 1.10145 fibrinogen C domain containing 1 FIBCD1 NM_032843 9.06E-08 8.35E-08 1.07084 4.71E-08 1.12747 PYD and CARD domain containing PYCARD NM_013258 /// NM_1451822.25E-06 /// NM_1451832.54E-06 1.07179 7.24E-07 1.20777 RAP2B, member of RAS oncogene family RAP2B NM_002886 6.33E-08 7.77E-08 1.07268 4.28E-08 1.13162 seizure related 6 homolog (mouse)-like 2 SEZ6L2 NM_012410 /// NM_2015751.07E-06 3.35E-07 1.07381 8.00E-07 0.989989 GLI pathogenesis-related 1 (glioma) GLIPR1 NM_006851 1.78E-09 9.55E-07 1.0741 6.68E-07 1.11088 Seven in absentia homolog 1 (Drosophila) SIAH1 NM_001006610 /// NM_0030311.28E-07 5.01E-08 1.07455 5.92E-08 1.05858 cathepsin B CTSB NM_001908 /// NM_1477801.48E-09 /// NM_1477814.93E-10 /// NM_147782 ///1.07491 NM_147783 1.20E-09 0.995725 hypothetical protein LOC340109 LOC340109 XM_379322 1.86E-09 5.11E-06 1.07516 5.41E-07 1.33306 ------1.29E-10 1.18E-08 1.07616 2.16E-08 1.01964 membrane-associated ring finger (C3HC4) 2 3-Mar NM_001005415 /// NM_0010054162.78E-10 /// NM_0164964.98E-09 1.0764 1.45E-09 1.19838 chondroitin sulfate GalNAcT-2 GALNACT-2 NM_018590 1.17E-07 1.37E-07 1.07737 3.42E-08 1.22107 protein C receptor, endothelial (EPCR) PROCR NM_006404 5.99E-10 1.00E-08 1.07753 7.75E-09 1.10217 LOC440702 --- XM_496425 9.39E-09 1.29E-07 1.07802 1.68E-07 1.05268 CD58 antigen, (lymphocyte function-associated antigen 3) CD58 NM_001779 7.57E-12 3.90E-12 1.07834 8.74E-12 1.00715 nipsnap homolog 3A (C. elegans) /// nipsnap homolog 3A (C. elegans) NIPSNAP3A NM_015469 1.25E-07 6.13E-08 1.07838 5.50E-08 1.08902 CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, E CD59 NM_000611 /// NM_2033292.17E-11 /// NM_2033308.35E-11 /// NM_203331 1.07866 3.30E-11 1.16758 muscleblind-like 2 (Drosophila) MBNL2 NM_144778 /// NM_2073041.83E-11 9.44E-12 1.08002 2.02E-11 1.01257 U2AF homology motif (UHM) kinase 1 UHMK1 NM_175866 1.09E-12 8.25E-11 1.08041 1.20E-10 1.04615 Pannexin 1 PANX1 NM_015368 5.22E-09 5.06E-09 1.08077 1.07E-08 1.01194 zinc finger protein 514 ZNF514 NM_032788 2.97E-07 2.18E-07 1.08117 2.23E-07 1.07928 Meis1, myeloid ecotropic viral integration site 1 homolog 4 (mouse) MEIS4 NR_002211 0.000279661 0.000211944 1.08136 5.79E-05 1.25078 T-box 3 (ulnar mammary syndrome) TBX3 NM_005996 /// NM_0165691.65E-11 1.70E-10 1.08137 2.99E-11 1.25442 BCL2/adenovirus E1B 19kDa interacting protein 3-like /// BCL2/adenovirus E1B 19k BNIP3L NM_004331 1.70E-07 7.89E-07 1.08154 1.97E-07 1.2298 Yip1 domain family, member 4 YIPF4 NM_032312 2.57E-07 1.65E-07 1.08245 9.30E-08 1.1406 keratin 15 KRT15 NM_002275 5.74E-09 2.94E-07 1.08373 1.15E-07 1.18147 hippocalcin-like 1 HPCAL1 NM_002149 /// NM_1344211.31E-09 6.55E-09 1.08381 1.47E-08 1.00922 CAAX box 1 CXX1 NM_003928 4.05E-10 2.84E-10 1.08424 1.28E-10 1.16094 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphor SEMA3B NM_001005914 /// NM_0046361.66E-08 1.66E-08 1.08795 1.29E-08 1.11249 hypothetical protein FLJ38725 FLJ38725 NM_153218 2.19E-08 3.07E-05 1.08866 2.77E-05 1.10027 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subf SMARCA2 NM_003070 /// NM_1390451.55E-06 3.37E-06 1.08926 2.96E-07 1.37101 solute carrier family 44, member 1 SLC44A1 NM_022109 /// NM_0805461.52E-10 6.07E-10 1.09228 9.15E-10 1.05426 aldehyde dehydrogenase 9 family, member A1 ALDH9A1 NM_000696 3.32E-10 1.03E-09 1.09231 1.15E-09 1.08236 ubiquitin-conjugating enzyme E2Q (putative) 2 UBE2Q2 NM_173469 1.62E-09 8.24E-08 1.09238 5.93E-08 1.12522 secretory carrier membrane protein 1 SCAMP1 NM_004866 /// NM_0528221.37E-10 4.51E-08 1.09261 4.77E-09 1.33323 transmembrane protein 57 /// similar to hypothetical protein MGC17347 TMEM57 /// LOC159090NM_018202 /// NM_1452847.47E-09 3.49E-08 1.09304 1.59E-08 1.17232 similar to hypothetical protein MGC17347 LOC159090 NM_145284 4.80E-09 1.01E-08 1.09347 5.30E-09 1.15748 solute carrier family 6 (neurotransmitter transporter, taurine), member 6 SLC6A6 NM_003043 2.71E-10 7.16E-11 1.09367 2.17E-10 0.994739 ------5.46E-11 2.01E-09 1.09519 5.66E-11 1.48935 SAR1 gene homolog B (S. cerevisiae) SAR1B NM_001033503 /// NM_0161033.91E-08 4.46E-08 1.09622 8.43E-09 1.27093 chromosome 9 open reading frame 150 C9orf150 NM_203403 6.30E-10 9.45E-10 1.09715 6.54E-10 1.13264 glucocorticoid induced transcript 1 GLCCI1 NM_138426 1.36E-10 3.50E-08 1.09789 5.03E-08 1.06266 chromosome 10 open reading frame 38 C10orf38 NM_001010924 1.96E-15 1.95E-15 1.09915 4.68E-16 1.23846 CD58 antigen, (lymphocyte function-associated antigen 3) CD58 NM_001779 3.00E-07 2.68E-07 1.09967 1.44E-07 1.16387 histone 1, H2bc HIST1H2BC NM_003526 6.08E-10 5.25E-07 1.10077 5.98E-07 1.08744 dystonin DST NM_001723 /// NM_0155481.22E-07 /// NM_0203883.54E-08 /// NM_183380 1.1009 8.94E-08 1.01275 Chaperonin containing TCP1, subunit 8 (theta) FMO5 NM_001461 2.88E-06 1.38E-06 1.10258 4.45E-06 0.984568 CD36 antigen (collagen type I receptor, thrombospondin receptor) CD36 NM_000072 /// NM_0010015473.24E-06 /// NM_0010015482.15E-06 1.10269 9.65E-07 1.19014 pleckstrin homology-like domain, family B, member 3 PHLDB3 NM_198850 5.98E-09 3.20E-08 1.103 7.80E-08 1.01836 tetratricopeptide repeat domain 3 TTC3 NM_001001894 /// NM_0033162.57E-11 7.68E-10 1.10393 4.94E-10 1.14679 thymosin, beta 4, X-linked /// thymosin-like 3 TMSB4X /// TMSL3NM_021109 /// NM_1830491.36E-08 5.00E-09 1.10425 7.80E-09 1.06198 mucin 13, epithelial transmembrane MUC13 NM_033049 8.46E-06 1.31E-05 1.10606 1.33E-06 1.38414 KIAA0802 KIAA0802 NM_015210 2.44E-08 1.58E-08 1.10786 7.16E-09 1.18796 Platelet-derived growth factor alpha polypeptide PDGFA NM_002607 /// NM_0330236.10E-10 3.19E-09 1.10807 7.25E-09 1.03113 interferon, alpha-inducible protein (clone IFI-6-16) G1P3 NM_002038 /// NM_0228722.33E-06 /// NM_0228733.25E-06 1.10877 2.49E-06 1.13762 3'-phosphoadenosine 5'-phosphosulfate synthase 2 PAPSS2 NM_001015880 /// NM_0046701.64E-10 1.78E-07 1.10989 7.50E-07 0.971433 epithelial membrane protein 2 EMP2 NM_001424 1.96E-09 1.26E-08 1.11024 2.48E-10 1.56284 hypothetical protein Ells1 Ells1 NM_152793 1.19E-07 2.02E-07 1.11126 6.72E-08 1.22835 laminin, beta 2 (laminin S) LAMB2 NM_002292 2.78E-05 6.75E-06 1.11212 2.07E-05 0.992768 membrane-bound transcription factor peptidase, site 1 MBTPS1 NM_003791 /// NM_2012685.04E-11 1.26E-10 1.11338 1.14E-10 1.12308 latent transforming growth factor beta binding protein 3 LTBP3 NM_021070 1.68E-07 1.41E-06 1.11437 3.71E-07 1.26332 transforming, acidic coiled-coil containing protein 2 TACC2 NM_006997 /// NM_2068609.88E-09 /// NM_2068614.92E-08 /// NM_206862 1.1147 2.77E-09 1.43759 chromosome X open reading frame 12 CXorf12 NM_003492 4.93E-08 1.22E-08 1.115 4.69E-08 0.988918 membrane-associated ring finger (C3HC4) 8 9-Mar NM_001002265 /// NM_0010022662.38E-10 /// NM_1450218.74E-10 1.11615 2.05E-09 1.03663 hydroxysteroid (17-beta) dehydrogenase 2 HSD17B2 NM_002153 7.05E-11 7.34E-09 1.11732 3.46E-08 0.973922 spectrin, alpha, non-erythrocytic 1 (alpha-fodrin) SPTAN1 NM_003127 7.00E-09 4.07E-09 1.11743 2.39E-08 0.955995 nucleolar protein 3 (apoptosis repressor with CARD domain) NOL3 NM_003946 8.79E-08 2.01E-08 1.11843 8.45E-08 0.98338 telomerase-associated protein 1 TEP1 NM_007110 8.88E-11 9.87E-08 1.12028 1.20E-07 1.10035 sulfotransferase family, cytosolic, 1C, member 1 SULT1C1 NM_001056 /// NM_1768251.55E-14 2.02E-10 1.12066 6.61E-10 1.01202 Metallothionein 1M MT1K NM_176870 1.82E-08 8.42E-09 1.1208 7.52E-09 1.132 spermidine/spermine N1-acetyltransferase SAT NM_002970 1.62E-12 1.10E-12 1.12109 5.37E-13 1.19063 myosin VA (heavy polypeptide 12, myoxin) MYO5A NM_000259 5.58E-07 5.67E-07 1.1214 9.80E-07 1.06529 DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 DDX58 NM_014314 4.85E-10 4.37E-10 1.122 5.65E-10 1.09736 AT rich interactive domain 5B (MRF1-like) ARID5B NM_032199 1.38E-12 5.51E-12 1.12408 1.53E-11 1.03066 CD58 antigen, (lymphocyte function-associated antigen 3) CD58 NM_001779 1.25E-10 6.07E-11 1.12464 1.80E-10 1.02483 complement component 1, s subcomponent C1S NM_001734 /// NM_2014421.69E-10 7.61E-09 1.12548 1.57E-08 1.05567 retinol binding protein 1, cellular RBP1 NM_002899 1.37E-07 3.39E-07 1.12672 3.78E-08 1.37526 histone 1, H3h HIST1H3H NM_003536 1.70E-13 1.69E-09 1.12761 8.39E-10 1.19798 CD58 antigen, (lymphocyte function-associated antigen 3) CD58 NM_001779 1.08E-08 5.73E-09 1.12868 1.50E-08 1.03671 glycerol kinase GK NM_000167 /// NM_2033915.53E-11 2.85E-10 1.12873 6.69E-10 1.04869 calcium channel, voltage-dependent, alpha 2/delta subunit 4 CACNA2D4 NM_001005737 /// NM_0010057667.62E-10 /// NM_1723644.44E-08 1.12887 1.99E-08 1.21235 limb region 1 homolog (mouse) LMBR1 NM_022458 1.29E-07 3.43E-07 1.12926 7.48E-08 1.29779 Transcribed locus ------4.26E-07 1.01E-07 1.13021 4.36E-07 0.98821 p53 target zinc finger protein WIG1 NM_022470 /// NM_1522404.19E-07 4.04E-07 1.13146 1.49E-07 1.23994 CD58 antigen, (lymphocyte function-associated antigen 3) /// CD58 antigen, (lymp CD58 NM_001779 5.41E-11 2.65E-11 1.13163 8.26E-11 1.02705 dystonin DST NM_001723 /// NM_0155484.48E-08 /// NM_0203881.17E-08 /// NM_183380 1.13269 3.32E-08 1.03287 SMAD specific E3 ubiquitin protein ligase 2 SMURF2 NM_022739 6.09E-10 3.24E-09 1.13324 6.38E-10 1.30505 CD99 antigen-like 2 CD99L2 NM_031462 /// NM_1344452.80E-09 /// NM_1344468.98E-09 1.13353 1.22E-08 1.1037 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

transmembrane and coiled-coil domains 3 TMCO3 NM_017905 2.38E-10 8.22E-11 1.13479 1.29E-10 1.092 hypothetical protein FLJ11000 FLJ11000 NM_018295 7.90E-10 2.13E-10 1.13544 8.14E-10 1.0115 metallothionein 1F (functional) MT1F NM_005949 3.06E-09 1.72E-09 1.13587 1.10E-09 1.18038 serine carboxypeptidase 1 SCPEP1 NM_021626 7.79E-10 6.39E-10 1.13621 1.20E-09 1.07615 Homo sapiens, clone IMAGE:5225774, mRNA ------8.03E-09 9.19E-09 1.13689 3.07E-09 1.25173 purine-rich element binding protein B PURB NM_033224 2.94E-08 1.42E-08 1.13711 1.80E-08 1.11366 sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) SGCB NM_000232 4.40E-11 3.82E-11 1.1396 1.27E-11 1.25105 B-cell translocation gene 1, anti-proliferative BTG1 NM_001731 6.22E-09 7.43E-09 1.13971 3.23E-09 1.22582 muscleblind-like 2 (Drosophila) MBNL2 NM_144778 /// NM_2073042.38E-09 7.96E-10 1.14159 1.30E-09 1.09427 tetratricopeptide repeat domain 7B TTC7B NM_001010854 7.35E-09 2.61E-07 1.14337 1.17E-07 1.23061 PTEN induced putative kinase 1 PINK1 NM_032409 1.62E-10 5.12E-10 1.14481 2.66E-10 1.21091 pleckstrin homology domain containing, family H (with MyTH4 domain) member 2 PLEKHH2 NM_172069 6.58E-06 5.74E-06 1.14542 1.42E-06 1.31153 Transcribed locus, weakly similar to XP_510104.1 PREDICTED: similar to hypotheti ------3.71E-07 1.82E-06 1.14566 4.35E-06 1.05288 CGI-116 protein CGI-116 NM_016053 7.37E-09 3.16E-08 1.1469 8.07E-08 1.05429 dickkopf homolog 3 (Xenopus laevis) DKK3 NM_001018057 /// NM_0132533.62E-07 /// NM_0158812.03E-07 1.14709 2.21E-07 1.13813 Hypothetical protein LOC201895 LOC201895 NM_174921 4.60E-14 8.42E-12 1.14874 2.77E-11 1.03836 drebrin 1 DBN1 NM_004395 /// NM_0808814.98E-09 4.19E-09 1.14944 1.46E-09 1.26036 popeye domain containing 3 POPDC3 NM_022361 5.62E-07 3.44E-06 1.1499 3.06E-06 1.16318 histone 1, H1c HIST1H1C NM_005319 4.89E-13 3.27E-08 1.15016 6.73E-08 1.07789 Hypothetical protein MGC34646 MGC34646 NM_173519 2.11E-10 1.29E-10 1.15099 1.26E-10 1.15321 forkhead box O3A FOXO3A NM_001455 /// NM_2015593.57E-07 1.54E-07 1.15114 1.59E-07 1.14812 protein kinase C, alpha PRKCA NM_002737 2.27E-10 3.11E-10 1.15164 1.66E-09 0.996495 aquaporin 8 AQP8 NM_001169 6.38E-05 6.61E-05 1.15217 3.72E-05 1.2257 CDNA FLJ31683 fis, clone NT2RI2005353 ------2.04E-11 1.69E-09 1.15358 3.69E-09 1.07735 chromosome 16 open reading frame 52 C16orf52 NM_173501 3.18E-10 4.61E-09 1.15414 2.22E-09 1.2302 granulin GRN NM_001012479 /// NM_0020874.66E-11 9.93E-11 1.15454 8.18E-11 1.17385 secretory carrier membrane protein 1 SCAMP1 NM_004866 /// NM_0528225.81E-10 9.60E-08 1.15471 3.89E-09 1.53543 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), membe SERPINA3 NM_001085 5.67E-14 7.55E-09 1.15511 4.82E-10 1.46749 ATP-binding cassette, sub-family C (CFTR/MRP), member 3 ABCC3 NM_003786 /// NM_0200371.27E-06 /// NM_0200381.54E-06 1.15565 1.65E-06 1.14781 glucocorticoid induced transcript 1 GLCCI1 NM_138426 3.08E-07 8.41E-07 1.15637 1.09E-06 1.12838 KIAA1815 KIAA1815 NM_024896 3.12E-14 5.48E-13 1.1565 3.06E-13 1.21476 leukotriene B4 12-hydroxydehydrogenase LTB4DH NM_012212 1.19E-15 3.87E-12 1.15695 1.74E-11 1.01839 caveolin 2 CAV2 NM_001233 /// NM_1982121.51E-06 4.54E-07 1.15701 1.56E-06 1.0298 decay accelerating factor for complement (CD55, Cromer blood group system) DAF NM_000574 2.64E-10 3.30E-09 1.15758 5.36E-10 1.35512 zinc finger and BTB domain containing 20 ZBTB20 NM_015642 1.06E-05 9.28E-05 1.15986 0.000199498 1.0635 zinc finger protein 83 (HPF1) ZNF83 NM_018300 0.000122319 6.31E-05 1.16183 6.72E-05 1.15394 iduronate 2-sulfatase (Hunter syndrome) IDS NM_000202 /// NM_0061232.17E-06 3.06E-06 1.16411 1.10E-06 1.28436 SMAD, mothers against DPP homolog 2 (Drosophila) SMAD2 NM_001003652 /// NM_0059012.53E-09 2.22E-08 1.16571 2.39E-09 1.418 sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphor SEMA3G NM_020163 3.62E-10 8.36E-09 1.16673 7.01E-08 0.965551 Inositol polyphosphate-4-phosphatase, type I, 107kDa INPP4A NM_001566 /// NM_0040271.13E-05 1.47E-05 1.1675 5.37E-05 1.01954 Zinc finger protein 117 (HPF9) ZNF117 NM_024498 3.15E-06 1.67E-05 1.16979 3.69E-05 1.07671 tweety homolog 2 (Drosophila) TTYH2 NM_032646 /// NM_0528693.32E-06 8.44E-07 1.17068 2.44E-06 1.05806 kelch-like 24 (Drosophila) KLHL24 NM_017644 1.52E-07 6.98E-08 1.17077 7.43E-08 1.16426 proline-rich nuclear receptor coactivator 1 PNRC1 NM_006813 2.73E-09 3.23E-09 1.17168 1.63E-09 1.24373 vanin 1 /// vanin 1 VNN1 NM_004666 2.48E-06 1.16E-06 1.17219 1.96E-06 1.11501 l(3)mbt-like (Drosophila) L3MBTL NM_015478 /// NM_0321076.83E-07 3.93E-06 1.17257 3.19E-06 1.19657 tribbles homolog 2 (Drosophila) TRIB2 NM_021643 1.93E-11 8.20E-09 1.1727 9.76E-09 1.15484 excision repair cross-complementing rodent repair deficiency, complementation gr ERCC1 NM_001983 /// NM_2020014.22E-10 2.55E-10 1.17436 1.30E-10 1.24396 sphingomyelin phosphodiesterase 1, acid lysosomal (acid sphingomyelinase) SMPD1 NM_000543 /// NM_0010075932.75E-09 1.04E-09 1.17538 5.37E-09 1.0185 CDNA FLJ34585 fis, clone KIDNE2008758 ------4.16E-07 1.88E-07 1.17714 6.80E-07 1.04493 tumor necrosis factor receptor superfamily, member 11a, NFKB activator TNFRSF11A NM_003839 5.53E-06 4.61E-06 1.17727 2.02E-05 1.01448 Inositol polyphosphate-4-phosphatase, type I, 107kDa INPP4A NM_001566 /// NM_0040271.13E-07 3.45E-07 1.17758 2.50E-07 1.21314 homer homolog 3 (Drosophila) HOMER3 NM_004838 6.08E-09 2.70E-09 1.17762 2.40E-09 1.18983 aldehyde oxidase 1 AOX1 NM_001159 3.39E-10 3.44E-09 1.17865 7.43E-09 1.10169 hypothetical protein LOC283852 LOC283852 --- 1.10E-11 2.93E-11 1.17871 1.37E-11 1.25777 PREDICTED: Homo sapiens similar to GKP3 protein (LOC201989), mRNA ------1.42E-07 8.31E-07 1.17922 2.90E-06 1.04638 regulating synaptic membrane exocytosis 3 RIMS3 NM_014747 1.62E-10 8.88E-10 1.17982 4.12E-09 1.03227 activated leukocyte cell adhesion molecule ALCAM NM_001627 1.47E-10 1.08E-08 1.17985 1.71E-10 1.69029 integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) ITGA2 NM_002203 5.62E-07 4.63E-07 1.18025 2.40E-06 1.01016 SH3 domain binding glutamic acid-rich protein like 3 /// SH3 domain binding glut SH3BGRL3 NM_031286 1.69E-11 2.13E-11 1.18164 1.74E-11 1.20237 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

transmembrane and coiled-coil domains 3 TMCO3 NM_017905 2.71E-08 6.02E-09 1.18301 2.53E-08 1.04217 ATM/ATR-Substrate Chk2-Interacting Zn2+-finger protein ASCIZ NM_015251 1.63E-15 3.53E-13 1.18317 9.61E-14 1.32007 B-cell translocation gene 1, anti-proliferative BTG1 NM_001731 9.49E-09 2.34E-08 1.18466 8.81E-09 1.29168 kinesin family member C2 KIFC2 NM_145754 2.15E-07 2.92E-07 1.18519 1.07E-07 1.29924 flavin containing monooxygenase 5 FMO5 NM_001461 2.02E-09 1.82E-09 1.18766 1.78E-09 1.19034 hexokinase 1 HK1 NM_000188 /// NM_0334969.02E-09 /// NM_0334979.82E-09 /// NM_033498 ///1.18859 NM_033500 2.28E-09 1.35063 GABA(A) receptor-associated protein like 1 /// GABA(A) receptors associated prot GABARAPL1 /// GABARAPL3NM_031412 4.63E-07 1.57E-07 1.18871 1.10E-06 0.992428 glycerol kinase GK NM_000167 /// NM_2033913.67E-12 1.72E-11 1.19149 4.59E-11 1.09583 armadillo repeat containing, X-linked 3 ARMCX3 NM_016607 /// NM_1779474.75E-09 /// NM_1779483.16E-09 1.1934 1.62E-09 1.26478 guanine nucleotide binding protein (G protein), beta polypeptide 1 GNB1 NM_002074 9.56E-15 1.06E-12 1.19388 8.21E-14 1.48065 ras homolog gene family, member Q RHOQ NM_012249 4.22E-13 5.12E-11 1.19507 2.14E-11 1.28717 metallothionein 1X MT1X NM_005952 4.56E-08 1.27E-08 1.19571 3.14E-08 1.10292 STEAP family member 3 STEAP3 NM_001008410 /// NM_0182346.33E-07 /// NM_1829155.60E-06 1.19673 2.18E-07 1.62884 bromodomain adjacent to zinc finger domain, 2B BAZ2B NM_013450 6.87E-09 3.10E-09 1.19974 2.99E-09 1.20354 paired box gene 6 (aniridia, keratitis) PAX6 NM_000280 /// NM_0016042.27E-10 2.87E-10 1.20157 1.29E-10 1.28711 glycerol kinase GK NM_000167 /// NM_2033911.02E-13 6.28E-14 1.20283 1.24E-13 1.13638 trophoblast-derived noncoding RNA TncRNA --- 5.78E-11 3.76E-09 1.20297 2.50E-09 1.24647 S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murin S100A4 NM_002961 /// NM_0195542.61E-08 3.26E-08 1.20327 1.75E-08 1.27156 protein tyrosine phosphatase type IVA, member 1 PTP4A1 NM_003463 3.51E-14 1.60E-12 1.20372 8.94E-14 1.53462 lectin, galactoside-binding, soluble, 1 (galectin 1) LGALS1 NM_002305 2.55E-11 9.84E-12 1.20579 1.28E-11 1.1795 aquaporin 3 AQP3 NM_004925 3.78E-09 2.14E-09 1.20684 2.27E-08 0.98054 CD109 antigen (Gov platelet alloantigens) CD109 NM_133493 1.02E-10 3.59E-10 1.20712 5.11E-10 1.17109 decay accelerating factor for complement (CD55, Cromer blood group system) DAF NM_000574 5.01E-11 3.83E-10 1.20919 1.04E-10 1.35231 transmembrane protein 16K TMEM16K NM_018075 2.06E-09 1.45E-09 1.21115 7.05E-10 1.28899 angiotensin II receptor, type 1 AGTR1 NM_000685 /// NM_0048351.61E-06 /// NM_0095851.41E-06 /// NM_031850 ///1.21128 NM_032049 5.08E-06 1.06999 solute carrier family 16 (monocarboxylic acid transporters), member 6 SLC16A6 NM_004694 1.20E-07 3.18E-05 1.21221 4.67E-06 1.47305 alpha-2-glycoprotein 1, zinc AZGP1 NM_001185 3.93E-09 2.01E-09 1.21479 1.37E-09 1.2561 GLI pathogenesis-related 1 (glioma) GLIPR1 NM_006851 3.53E-14 3.67E-13 1.21602 4.31E-13 1.1998 hypothetical protein FLJ31401 FLJ31401 --- 2.02E-08 4.35E-08 1.22004 2.30E-07 1.04926 filamin binding LIM protein 1 FBLIM1 NM_001024215 /// NM_0010242161.29E-08 /// NM_0175561.57E-08 1.221 1.26E-08 1.24487 granulin GRN NM_001012479 /// NM_0020871.41E-11 3.55E-11 1.22156 3.67E-11 1.21825 progestin and adipoQ receptor family member V PAQR5 NM_017705 4.48E-05 2.37E-05 1.22172 4.43E-05 1.14399 tubulin, beta 2 TUBB2 NM_001069 6.19E-12 4.52E-12 1.22189 3.77E-12 1.24071 collagen, type VI, alpha 1 COL6A1 NM_001848 4.67E-07 1.57E-07 1.22682 2.81E-07 1.16279 Ubiquitin B (UBB) mRNA, 3' UTR and genetic suppressor element ------6.24E-10 9.16E-08 1.228 1.39E-08 1.45376 Ras-related GTP binding D RRAGD NM_021244 2.82E-08 2.51E-07 1.22869 2.07E-06 1.00763 CD99 antigen-like 2 CD99L2 NM_031462 /// NM_1344454.86E-08 /// NM_1344466.61E-07 1.22888 2.34E-07 1.35294 Nuclear factor I/B NFIB NM_005596 4.89E-07 4.13E-07 1.22983 1.93E-07 1.31926 transmembrane 4 L six family member 5 TM4SF5 NM_003963 1.47E-07 1.82E-07 1.2308 2.19E-07 1.21054 adenosine monophosphate deaminase (isoform E) AMPD3 NM_000480 /// NM_0010253896.65E-11 /// NM_0010253908.68E-11 1.23108 4.65E-11 1.29842 iduronate 2-sulfatase (Hunter syndrome) IDS NM_000202 /// NM_0061231.30E-10 4.80E-09 1.23173 1.28E-09 1.38213 matrix-remodelling associated 7 MXRA7 NM_001008528 /// NM_0010085295.16E-11 /// NM_1985302.53E-11 1.23205 1.94E-11 1.26036 pleckstrin homology domain containing, family M (with RUN domain) member 1 PLEKHM1 NM_014798 2.72E-09 4.79E-09 1.23357 3.01E-09 1.28461 Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) ITGA2 NM_002203 1.68E-10 2.57E-11 1.23366 3.51E-10 0.98638 mitogen-activated protein kinase kinase kinase 8 MAP3K8 NM_005204 1.54E-08 6.53E-07 1.23521 2.43E-07 1.35357 transient receptor potential cation channel, subfamily V, member 2 TRPV2 NM_016113 3.26E-09 1.74E-09 1.23606 6.31E-09 1.10484 Spermidine/spermine N1-acetyltransferase SAT NM_002970 1.64E-10 1.95E-10 1.23644 4.51E-11 1.40163 glypican 1 GPC1 NM_002081 5.12E-13 1.67E-10 1.23645 9.95E-10 1.06052 Hypothetical protein FLJ20674 FLJ20674 NM_019086 1.27E-06 4.02E-07 1.23698 8.78E-07 1.15004 chromosome 20 open reading frame 19 C20orf19 NM_018474 3.92E-06 7.32E-06 1.23738 2.99E-05 1.07144 hypothetical protein FLJ90586 FLJ90586 NM_153345 1.36E-08 4.33E-09 1.23811 7.77E-09 1.17611 KIAA1815 KIAA1815 NM_024896 1.14E-13 3.58E-10 1.23835 8.30E-11 1.40381 receptor interacting protein kinase 5 RIPK5 NM_015375 /// NM_1994623.05E-11 5.56E-10 1.23932 8.01E-10 1.20086 mesothelin MSLN NM_005823 /// NM_0134043.62E-07 9.69E-08 1.24059 3.48E-07 1.10367 glucosamine (N-acetyl)-6-sulfatase (Sanfilippo disease IIID) GNS NM_002076 2.29E-11 3.23E-11 1.24077 2.77E-11 1.25719 microtubule associated monoxygenase, calponin and LIM domain containing 2 MICAL2 NM_014632 4.82E-10 2.16E-09 1.24126 4.13E-10 1.43223 solute carrier family 23 (nucleobase transporters), member 2 SLC23A2 NM_005116 /// NM_2033273.30E-09 1.29E-09 1.24244 1.56E-09 1.22214 interferon, alpha-inducible protein (clone IFI-15K) G1P2 NM_005101 1.35E-09 1.06E-09 1.24456 3.42E-10 1.3722 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta poly YWHAH NM_003405 4.71E-12 1.38E-10 1.24505 5.11E-12 1.64805 ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) UBE2H NM_003344 /// NM_1826971.14E-12 4.36E-12 1.24544 5.56E-12 1.22001 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

protein tyrosine phosphatase, receptor type, M PTPRM NM_002845 5.47E-11 4.84E-10 1.24734 7.32E-10 1.20356 hypothetical protein LOC201175 LOC201175 NM_174919 5.11E-08 4.73E-07 1.24791 1.58E-07 1.38074 granulin GRN NM_001012479 /// NM_0020871.53E-11 4.08E-11 1.24895 6.83E-11 1.19523 osteopetrosis associated transmembrane protein 1 OSTM1 NM_014028 1.01E-08 5.00E-09 1.25149 7.71E-08 0.981373 hypothetical protein FLJ20719 FLJ20719 XM_373827 /// XM_4984276.94E-07 3.73E-07 1.25166 2.14E-07 1.31721 cathepsin D (lysosomal aspartyl peptidase) CTSD NM_001909 7.32E-08 9.94E-08 1.25172 4.38E-07 1.09249 aquaporin 11 AQP11 NM_173039 4.33E-10 2.27E-09 1.25176 5.37E-09 1.16097 guanine nucleotide binding protein (G protein), beta 5 GNB5 NM_006578 /// NM_0161947.35E-10 3.43E-09 1.25607 9.88E-09 1.14472 clathrin, light polypeptide (Lcb) CLTB NM_001834 /// NM_0070971.01E-09 8.11E-10 1.25749 2.96E-10 1.37169 galactosamine (N-acetyl)-6-sulfate sulfatase (Morquio syndrome, mucopolysacchari GALNS NM_000512 1.91E-10 2.78E-10 1.2585 2.18E-10 1.28517 cAMP responsive element binding protein 5 CREB5 NM_001011666 /// NM_0049048.76E-05 /// NM_1828981.70E-05 /// NM_1828991.26138 0.00019333 0.969063 Ras-like without CAAX 1 RIT1 NM_006912 9.44E-08 7.22E-08 1.26144 5.11E-07 1.05443 biliverdin reductase B (flavin reductase (NADPH)) BLVRB NM_000713 1.92E-09 7.01E-10 1.26329 1.09E-09 1.2159 likely ortholog of mouse gene rich cluster, C10 gene GRCC10 NM_138425 5.67E-11 6.02E-11 1.264 1.75E-11 1.40435 tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediato TNFRSF14 NM_003820 4.52E-08 2.41E-07 1.26473 9.56E-07 1.11235 jun dimerization protein 2 JDP2 NM_130469 4.03E-05 2.55E-05 1.26492 1.07E-05 1.38224 drebrin 1 DBN1 NM_004395 /// NM_0808811.26E-09 3.90E-10 1.2678 8.03E-10 1.19121 CDNA FLJ11958 fis, clone HEMBB1000996 ------4.45E-06 1.54E-06 1.2683 2.99E-06 1.19018 Eukaryotic translation initiation factor 2C, 2 EIF2C2 NM_012154 2.12E-08 5.45E-06 1.26849 4.79E-07 1.60067 p53 target zinc finger protein WIG1 NM_022470 /// NM_1522401.33E-11 1.21E-10 1.26962 4.53E-11 1.38065 cytochrome P450, family 3, subfamily A, polypeptide 5 CYP3A5 NM_000777 1.56E-05 0.000127599 1.2698 0.000322432 1.14015 frizzled homolog 6 (Drosophila) FZD6 NM_003506 1.53E-09 2.69E-10 1.27348 2.31E-09 1.05744 FLJ20160 protein FLJ20160 NM_017694 5.02E-08 4.10E-08 1.27468 1.90E-08 1.36525 zinc finger protein 90 homolog (mouse) ZFP90 NM_133458 1.92E-08 1.57E-08 1.27657 1.93E-08 1.25376 CDNA FLJ31683 fis, clone NT2RI2005353 ------1.11E-11 7.31E-10 1.27672 5.39E-10 1.31069 dystrobrevin, alpha DTNA NM_001390 /// NM_0013911.42E-06 /// NM_0013925.52E-07 /// NM_032975 ///1.27676 NM_032978 /// 9.21E-07NM_032979 1.21692 hypothetical protein LOC284356 LOC284356 --- 5.78E-08 4.93E-08 1.27753 3.65E-08 1.31244 ATPase, Class I, type 8B, member 3 ATP8B3 NM_138813 5.70E-06 4.03E-06 1.27786 1.74E-06 1.38632 collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and rec COL7A1 NM_000094 9.08E-11 1.80E-08 1.27792 1.86E-08 1.27431 N-myc (and STAT) interactor NMI NM_004688 1.31E-08 7.76E-09 1.27806 1.68E-08 1.19367 Myristoylated alanine-rich protein kinase C substrate MARCKS NM_002356 7.63E-10 1.28E-09 1.27868 4.80E-09 1.1395 carnitine palmitoyltransferase 1A (liver) CPT1A NM_001031847 /// NM_0018763.31E-11 5.63E-09 1.27893 3.58E-08 1.08575 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type SERPINE1 NM_000602 5.85E-08 1.87E-08 1.27905 3.16E-08 1.22081 myosin ID MYO1D NM_015194 5.48E-10 2.42E-08 1.27976 1.78E-08 1.31532 metallothionein 1X MT1X NM_005952 8.22E-10 5.39E-10 1.28154 3.07E-10 1.34514 HSPC159 protein HSPC159 NM_014181 2.13E-12 6.02E-10 1.28202 4.75E-11 1.59394 keratin associated protein 1-5 KRTAP1-5 NM_031957 1.32E-09 8.04E-10 1.2832 2.27E-09 1.17264 NDRG family member 4 NDRG4 NM_020465 /// NM_0229101.32E-10 3.01E-10 1.28339 5.42E-09 0.998559 ATPase, Class II, type 9A ATP9A NM_006045 7.91E-11 1.13E-10 1.28369 2.04E-10 1.22053 lectin, galactoside-binding, soluble, 3 (galectin 3) /// galectin-3 internal gen LGALS3 /// GALIG NM_002306 /// NM_1943271.22E-14 5.25E-12 1.28449 2.68E-12 1.35981 SAR1 gene homolog B (S. cerevisiae) SAR1B NM_001033503 /// NM_0161031.94E-09 2.08E-09 1.28531 4.22E-10 1.47573 HMG-box transcription factor 1 HBP1 NM_012257 4.17E-11 3.05E-11 1.28577 1.33E-10 1.13415 family with sequence similarity 61, member A FAM61A NM_015578 4.10E-14 1.77E-11 1.28714 6.66E-14 2.06152 ------4.27E-09 2.91E-08 1.28985 1.52E-09 1.67266 tumor protein p53 inducible nuclear protein 1 TP53INP1 NM_033285 6.22E-13 4.80E-11 1.28998 1.67E-10 1.15933 cysteine-rich protein 1 (intestinal) CRIP1 NM_001311 7.40E-11 3.58E-10 1.29007 3.06E-10 1.30754 DnaJ (Hsp40) homolog, subfamily C, member 12 DNAJC12 NM_021800 /// NM_2012623.39E-09 1.96E-09 1.29119 1.53E-09 1.31962 solute carrier family 23 (nucleobase transporters), member 2 SLC23A2 NM_005116 /// NM_2033271.05E-10 2.36E-10 1.29162 8.27E-11 1.41278 insulin-like growth factor binding protein 1 IGFBP1 NM_000596 /// NM_0010130295.94E-11 2.96E-07 1.2922 2.55E-07 1.31025 cortactin CTTN NM_005231 /// NM_1385654.81E-10 1.11E-09 1.29359 2.95E-10 1.45003 tumor protein p53 inducible protein 3 TP53I3 NM_004881 /// NM_1471842.19E-15 1.06E-11 1.29455 8.87E-12 1.31462 optineurin OPTN NM_001008211 /// NM_0010082124.44E-09 /// NM_0010082131.84E-08 /// NM_0219801.29457 3.64E-07 0.988287 hypothetical protein LOC144501 LOC144501 NM_182507 6.93E-10 8.39E-10 1.29539 2.42E-10 1.44177 guanylate binding protein 3 GBP3 NM_018284 3.43E-07 2.73E-07 1.29775 2.62E-06 1.04856 glucosamine (N-acetyl)-6-sulfatase (Sanfilippo disease IIID) GNS NM_002076 1.87E-12 6.02E-13 1.29866 1.10E-12 1.23409 hypothetical protein LOC162993 LOC162993 XM_091914 2.31E-06 9.45E-07 1.29965 1.13E-06 1.27784 hypothetical protein FLJ31306 FLJ31306 XM_495990 8.58E-09 4.25E-08 1.30047 1.33E-07 1.17354 aspartate beta-hydroxylase ASPH NM_004318 /// NM_0201648.29E-15 /// NM_0324662.30E-15 /// NM_032467 ///1.30551 NM_032468 2.03E-14 1.08817 plasminogen activator, urokinase PLAU NM_002658 1.83E-08 2.13E-08 1.30583 4.88E-09 1.48719 Mitogen-activated protein kinase kinase 1 interacting protein 1 MAP2K1IP1 NM_021970 6.89E-11 1.79E-08 1.30955 4.16E-09 1.48911 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

WD repeat domain 45 WDR45 NM_001029896 /// NM_0070753.60E-11 3.74E-11 1.31347 1.89E-11 1.3918 arylacetamide deacetylase (esterase) AADAC NM_001086 1.55E-09 7.04E-09 1.31555 1.48E-08 1.23214 zinc finger protein 708 (KOX8) ZNF708 NM_021269 2.08E-06 1.11E-06 1.31696 6.36E-07 1.38737 decay accelerating factor for complement (CD55, Cromer blood group system) DAF NM_000574 8.59E-11 4.41E-10 1.31896 1.24E-10 1.47105 zinc finger, DHHC-type containing 2 ZDHHC2 NM_016353 1.21E-08 1.64E-08 1.31958 1.84E-08 1.30633 POU domain, class 6, transcription factor 1 ASH1L NM_018489 3.35E-11 3.97E-12 1.32202 1.49E-10 0.971196 Hypothetical protein FLJ14681 FLJ14681 NM_032824 1.94E-10 9.15E-10 1.32401 3.25E-10 1.44777 filamin binding LIM protein 1 FBLIM1 NM_001024215 /// NM_0010242167.05E-09 /// NM_0175569.70E-09 1.32575 4.88E-09 1.40823 zinc finger protein 680 ZNF680 NM_178558 4.56E-08 6.42E-08 1.32629 3.40E-07 1.13919 RAB31, member RAS oncogene family RAB31 NM_006868 2.99E-08 8.58E-07 1.32757 1.13E-06 1.29342 synaptotagmin-like 1 SYTL1 NM_032872 4.23E-13 4.12E-10 1.33096 1.01E-10 1.50202 hypothetical protein FLJ90586 FLJ90586 NM_153345 8.62E-06 3.37E-06 1.33277 7.23E-06 1.23618 iduronate 2-sulfatase (Hunter syndrome) IDS NM_000202 /// NM_0061232.68E-10 1.76E-09 1.33314 1.45E-09 1.35581 cytoplasmic linker 2 /// cytoplasmic linker 2 CYLN2 NM_003388 /// NM_0324212.74E-09 8.06E-09 1.33327 1.77E-08 1.24378 aldehyde oxidase 1 AOX1 NM_001159 2.20E-11 1.30E-10 1.33418 5.92E-10 1.17124 glycerol kinase GK NM_000167 /// NM_2033911.21E-09 4.68E-09 1.33916 8.47E-09 1.27116 solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 SLC7A11 NM_014331 7.51E-12 1.73E-09 1.34338 6.32E-08 0.977728 amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease) APP NM_000484 /// NM_2014132.95E-07 /// NM_2014142.04E-06 1.3441 3.58E-07 1.58371 hect domain and RLD 4 HERC4 NM_001017972 /// NM_0156019.41E-11 /// NM_0220791.11E-10 1.34553 9.91E-11 1.35908 CDNA FLJ34585 fis, clone KIDNE2008758 ------3.42E-05 0.000153116 1.34638 0.00211982 0.966822 trophoblast-derived noncoding RNA TncRNA --- 4.67E-11 3.09E-09 1.34786 1.45E-09 1.43953 ras homolog gene family, member Q RHOQ NM_012249 7.34E-10 4.04E-09 1.34944 1.50E-09 1.47147 Translocation associated membrane protein 1 TRAM1 NM_014294 5.41E-05 3.67E-05 1.35108 1.34E-05 1.50056 epithelial membrane protein 2 EMP2 NM_001424 1.44E-08 6.62E-08 1.35113 5.98E-09 1.67288 selenoprotein M SELM NM_080430 4.66E-06 1.93E-06 1.35259 4.28E-06 1.25179 aspartate beta-hydroxylase ASPH NM_004318 /// NM_0201646.39E-07 /// NM_0324665.81E-07 /// NM_032467 ///1.35265 NM_032468 4.54E-07 1.38422 ATP-binding cassette, sub-family C (CFTR/MRP), member 3 ABCC3 NM_003786 /// NM_0200373.30E-14 /// NM_0200387.28E-14 1.35469 1.66E-13 1.26379 Zinc finger protein 224 ZNF224 NM_013398 1.48E-08 6.39E-09 1.35814 8.29E-09 1.32743 androgen-induced 1 AIG1 NM_016108 3.09E-11 2.06E-11 1.36048 1.42E-11 1.40405 Interleukin 6 signal transducer (gp130, oncostatin M receptor) IL6ST NM_002184 /// NM_1757671.98E-13 3.87E-12 1.36059 1.51E-12 1.47343 limkain b1 LKAP NM_014647 2.92E-09 1.97E-09 1.36175 1.40E-09 1.40257 transmembrane protein 55A TMEM55A NM_018710 5.88E-07 5.04E-06 1.36309 5.14E-06 1.36039 hypothetical protein FLJ22313 FLJ22313 NM_022373 3.32E-09 1.98E-08 1.36354 1.67E-08 1.38424 chromosome 17 open reading frame 27 C17orf27 NM_020914 3.44E-08 8.52E-08 1.36658 1.50E-07 1.29773 plasminogen activator, urokinase /// plasminogen activator, urokinase PLAU NM_002658 8.88E-10 4.10E-10 1.36935 9.38E-10 1.27491 Transcribed locus, weakly similar to NP_872601.1 tetratricopeptide repeat protei ------1.86E-07 6.47E-08 1.37148 1.04E-07 1.31413 histone 2, H2be HIST2H2BE NM_003528 4.00E-13 6.64E-09 1.37284 9.45E-09 1.33085 rabaptin, RAB GTPase binding effector protein 2 RABEP2 NM_024816 1.26E-08 1.07E-08 1.37442 6.98E-09 1.4266 guanine nucleotide binding protein (G protein), beta polypeptide 1 GNB1 NM_002074 8.70E-14 6.46E-12 1.37477 5.66E-13 1.68863 ribonucleotide reductase M2 B (TP53 inducible) RRM2B NM_015713 3.18E-10 5.21E-10 1.37529 2.19E-10 1.48186 Thymidylate synthetase TYMS NM_001071 2.53E-07 2.63E-07 1.37621 1.84E-07 1.42224 solute carrier family 22 (organic cation transporter), member 18 SLC22A18 NM_002555 /// NM_1832335.03E-11 1.05E-10 1.37778 1.22E-10 1.36024 EGF-like-domain, multiple 5 EGFL5 XM_376905 3.84E-10 6.77E-11 1.37978 6.10E-10 1.14263 solute carrier family 44, member 5 SLC44A5 NM_152697 1.17E-14 5.59E-12 1.37991 2.86E-12 1.46056 Docking protein 6 DOK6 NM_152721 2.12E-07 3.91E-07 1.38056 2.57E-07 1.43497 t-complex-associated-testis-expressed 1-like TCTE1L NM_006520 2.08E-13 1.34E-10 1.38406 1.93E-10 1.34126 hypothetical protein LOC51315 LOC51315 NM_016618 2.66E-13 2.11E-12 1.38899 1.71E-12 1.41405 guanylate binding protein 2, interferon-inducible /// guanylate binding protein GBP2 NM_004120 2.07E-09 5.36E-09 1.38959 8.32E-09 1.33698 Trophoblast-derived noncoding RNA TncRNA --- 6.40E-11 6.29E-10 1.38984 8.02E-11 1.65853 solute carrier family 26, member 11 SLC26A11 NM_173626 4.71E-11 8.03E-11 1.39042 1.86E-10 1.29405 cytochrome P450, family 4, subfamily F, polypeptide 11 CYP4F11 NM_021187 8.61E-09 1.03E-09 1.39083 3.62E-08 1.01707 forkhead box O3A FOXO3A NM_001455 /// NM_2015591.76E-13 7.03E-13 1.39085 1.65E-13 1.57148 Transcribed locus ------1.65E-09 3.21E-09 1.39106 2.81E-08 1.14869 KIAA0934 KIAA0934 NM_014974 2.67E-09 1.50E-08 1.3917 1.18E-08 1.42199 Eukaryotic translation initiation factor 2C, 2 EIF2C2 NM_012154 1.03E-10 2.38E-07 1.39336 9.35E-09 1.86379 quaking homolog, KH domain RNA binding (mouse) QKI NM_006775 /// NM_2068532.76E-09 /// NM_2068541.18E-06 /// NM_206855 1.39583 1.01E-07 1.75235 transforming growth factor beta 1 induced transcript 1 TGFB1I1 NM_015927 4.83E-09 3.59E-09 1.39749 1.98E-09 1.47171 chromosome 8 open reading frame 1 C8orf1 NM_004337 2.92E-08 4.56E-08 1.39863 1.54E-08 1.54052 AXL receptor tyrosine kinase AXL NM_001699 /// NM_0219132.61E-10 1.71E-10 1.39927 1.05E-10 1.45862 chondroitin polymerizing factor CHPF NM_024536 7.71E-10 1.12E-08 1.40051 6.53E-09 1.46913 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

praja 2, RING-H2 motif containing PJA2 NM_014819 2.09E-09 1.23E-09 1.40519 6.67E-10 1.48163 chromosome 9 open reading frame 95 C9orf95 NM_017881 8.88E-13 8.43E-13 1.40627 2.80E-13 1.54301 hypothetical protein FLJ22833 FLJ22833 NM_001031716 /// NM_0228379.86E-11 5.65E-11 1.40952 1.18E-10 1.32375 Microtubule-associated protein 1B /// Homo sapiens, clone IMAGE:5535936, mRNA MAP1B NM_005909 /// NM_0320101.53E-10 5.95E-11 1.4099 6.13E-10 1.15423 La ribonucleoprotein domain family, member 6 LARP6 NM_018357 /// NM_1979584.35E-08 1.55E-07 1.41167 2.21E-08 1.68269 guanine deaminase GDA NM_004293 7.79E-10 6.71E-10 1.41639 4.49E-10 1.46626 interleukin 10 receptor, beta IL10RB NM_000628 3.35E-09 3.58E-09 1.41934 9.17E-10 1.59789 epithelial membrane protein 2 EMP2 NM_001424 2.04E-08 4.81E-08 1.4194 1.27E-08 1.59801 Follicular lymphoma variant translocation 1 FVT1 NM_002035 4.52E-07 4.43E-07 1.42152 1.24E-07 1.59769 sequestosome 1 SQSTM1 NM_003900 1.00E-11 7.72E-10 1.42458 3.04E-09 1.26475 zinc finger protein 432 ZNF432 NM_014650 1.67E-08 1.78E-08 1.42662 1.68E-08 1.43369 chromosome 17 open reading frame 27 C17orf27 NM_020914 2.16E-07 2.28E-07 1.42863 1.74E-06 1.1812 Ras homolog gene family, member Q RHOQ NM_012249 5.38E-14 8.47E-12 1.43393 4.35E-12 1.51713 yippee-like 3 (Drosophila) YPEL3 NM_031477 8.02E-12 3.80E-09 1.44075 8.59E-10 1.63942 PHD finger protein 20-like 1 PHF20L1 NM_016018 /// NM_0248781.76E-08 /// NM_0322054.36E-08 /// NM_198513 1.44163 6.34E-08 1.39385 cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) CDKN2B NM_004936 /// NM_0784875.79E-09 2.15E-09 1.44346 6.52E-09 1.31008 SRY (sex determining region Y)-box 4 SOX4 NM_003107 3.18E-14 1.69E-14 1.44385 1.32E-14 1.4742 SMAD specific E3 ubiquitin protein ligase 2 SMURF2 NM_022739 1.11E-06 1.34E-06 1.44386 1.32E-06 1.44573 heat shock 27kDa protein 1 HSPB1 NM_001540 2.45E-11 1.00E-11 1.44651 1.09E-11 1.43684 secretory carrier membrane protein 1 SCAMP1 NM_004866 /// NM_0528222.29E-09 2.11E-07 1.44932 4.62E-08 1.663 Transcribed locus, weakly similar to XP_517454.1 PREDICTED: similar to hypotheti ------1.63E-08 2.65E-09 1.45019 2.73E-08 1.18098 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); tran MLLT11 NM_006818 3.40E-09 9.88E-10 1.45139 3.05E-09 1.31583 caveolin 2 CAV2 NM_001233 /// NM_1982124.71E-06 3.10E-06 1.45429 1.65E-06 1.5453 ATP-binding cassette, sub-family C (CFTR/MRP), member 3 ABCC3 NM_003786 /// NM_0200372.64E-10 /// NM_0200384.15E-10 1.45534 9.16E-10 1.35905 CDNA FLJ38112 fis, clone D3OST2002272 ------4.27E-11 8.17E-09 1.46597 1.96E-09 1.66084 hypothetical gene supported by BC034933; BC068085 LOC440995 XM_498955 1.02E-10 1.95E-10 1.466 1.77E-10 1.47778 hypothetical protein LOC283666 LOC283666 --- 3.33E-09 1.53E-08 1.46601 1.74E-07 1.17815 Hypothetical protein MGC34646 MGC34646 NM_173519 2.05E-13 2.71E-13 1.47108 1.26E-13 1.56904 interferon-stimulated transcription factor 3, gamma 48kDa ISGF3G NM_006084 2.57E-11 9.20E-12 1.47627 2.00E-11 1.38204 hypothetical protein FLJ22833 FLJ22833 NM_001031716 /// NM_0228376.07E-10 3.87E-10 1.4928 1.09E-09 1.3647 thymidine kinase 2, mitochondrial TK2 NM_004614 1.95E-11 3.98E-10 1.49335 6.26E-11 1.74995 hypothetical protein FLJ22833 FLJ22833 NM_001031716 /// NM_0228374.87E-07 1.71E-07 1.49448 7.50E-07 1.30348 hypothetical protein FLJ22313 FLJ22313 NM_022373 8.11E-11 3.66E-10 1.49713 1.44E-10 1.62141 SRY (sex determining region Y)-box 4 SOX4 NM_003107 4.89E-07 1.15E-06 1.49922 7.56E-07 1.56012 TIMP metallopeptidase inhibitor 2 TIMP2 NM_003255 2.68E-11 1.07E-11 1.4993 1.54E-11 1.45373 Hypothetical protein LOC201895 LOC201895 NM_174921 3.16E-11 2.25E-09 1.49955 3.11E-08 1.18986 KIAA1618 KIAA1618 NM_020954 9.54E-14 7.32E-12 1.50172 1.06E-12 1.76827 cytochrome P450, family 4, subfamily F, polypeptide 3 CYP4F3 NM_000896 4.16E-11 1.45E-10 1.50238 4.86E-09 1.10842 SRY (sex determining region Y)-box 4 SOX4 NM_003107 7.29E-10 4.80E-10 1.50319 1.26E-09 1.3826 chromosome X open reading frame 45 CXorf45 NM_024810 3.06E-09 4.09E-08 1.50366 3.44E-08 1.52714 FOS-like antigen 2 FOSL2 NM_005253 2.57E-09 1.60E-08 1.50429 2.16E-08 1.46509 intercellular adhesion molecule 2 ICAM2 NM_000873 1.50E-08 1.25E-08 1.50475 5.05E-09 1.62991 protein tyrosine phosphatase, receptor type, N polypeptide 2 PTPRN2 NM_002847 /// NM_1308421.68E-08 /// NM_1308431.83E-07 1.50494 7.60E-08 1.62978 Interleukin 6 signal transducer (gp130, oncostatin M receptor) IL6ST NM_002184 /// NM_1757672.85E-12 2.66E-11 1.50507 9.73E-12 1.63921 neutrophil cytosolic factor 2 (65kDa, chronic granulomatous disease, autosomal 2 NCF2 NM_000433 3.99E-08 2.02E-08 1.50539 2.35E-08 1.48503 cytochrome P450, family 3, subfamily A, polypeptide 7 CYP3A7 NM_000765 6.36E-12 1.53E-09 1.50714 3.54E-09 1.40076 lectin, galactoside-binding, soluble, 7 (galectin 7) LGALS7 NM_002307 3.82E-09 3.00E-09 1.50808 9.54E-10 1.66599 spermidine/spermine N1-acetyltransferase SAT NM_002970 3.08E-12 1.80E-12 1.51176 1.32E-12 1.55167 benzodiazapine receptor (peripheral) BZRP NM_000714 /// NM_0073113.26E-08 1.70E-07 1.51252 1.40E-07 1.53938 trinucleotide repeat containing 6A TNRC6A NM_014494 /// NM_0208475.91E-12 3.11E-11 1.51826 3.68E-11 1.49671 methionine sulfoxide reductase B3 MSRB3 NM_001031679 /// NM_1980807.31E-07 1.09E-06 1.51882 3.43E-07 1.69177 sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) SGCB NM_000232 7.23E-12 5.03E-11 1.51945 3.53E-11 1.56623 G protein-coupled receptor 56 GPR56 NM_005682 /// NM_2015241.01E-12 /// NM_2015255.33E-10 1.52011 3.39E-10 1.58041 trophoblast glycoprotein TPBG NM_006670 4.60E-08 1.14E-07 1.52381 8.19E-09 1.92821 Kruppel-like factor 11 KLF11 NM_003597 1.12E-11 1.59E-11 1.52643 7.02E-12 1.63577 adenylate cyclase 7 ADCY7 NM_001114 1.35E-09 1.46E-09 1.53071 6.03E-09 1.3522 zinc finger and BTB domain containing 4 ZBTB4 NM_020899 1.77E-10 1.27E-09 1.53203 7.83E-09 1.30698 nidogen 1 NID1 NM_002508 3.69E-10 2.48E-10 1.53408 1.34E-10 1.61775 yippee-like 5 (Drosophila) YPEL5 NM_016061 3.76E-13 2.29E-13 1.53482 1.63E-13 1.5794 chromosome 9 open reading frame 152 C9orf152 NM_001012993 3.01E-10 2.03E-10 1.53497 1.08E-10 1.62088 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

FOS-like antigen 2 FOSL2 NM_005253 7.53E-12 3.28E-10 1.53565 5.12E-10 1.47792 histone 1, H2ac HIST1H2AC NM_003512 2.51E-11 1.79E-09 1.53819 1.11E-09 1.60288 chromosome 19 open reading frame 33 C19orf33 NM_033520 5.92E-10 1.71E-09 1.53877 2.52E-10 1.81547 trinucleotide repeat containing 8 TNRC8 --- 1.25E-07 1.15E-07 1.54284 1.56E-07 1.50008 PQ loop repeat containing 3 PQLC3 NM_152391 1.49E-08 1.91E-08 1.54661 8.24E-09 1.66617 matrix-remodelling associated 8 MXRA8 NM_032348 1.79E-09 9.23E-10 1.54848 6.46E-10 1.59695 RAB31, member RAS oncogene family RAB31 NM_006868 1.54E-09 1.34E-08 1.55298 1.51E-08 1.53635 tumor differentially expressed 2-like TDE2L NM_178865 2.43E-10 1.16E-09 1.55532 4.70E-10 1.68121 CDNA FLJ11754 fis, clone HEMBA1005588 ------2.46E-09 1.22E-09 1.56593 1.95E-09 1.50315 cell cycle progression 1 CCPG1 NM_004748 /// NM_0207392.80E-07 7.27E-08 1.56657 4.07E-07 1.33827 mucin 15 MUC15 NM_145650 1.38E-08 3.09E-08 1.56962 1.31E-08 1.69449 TBC1 domain family, member 3 /// TBC1 domain family, member 3C TBC1D3 /// TBC1D3CNM_001001418 /// NM_0322589.16E-10 9.13E-10 1.57541 5.45E-10 1.64731 melanoma cell adhesion molecule MCAM NM_006500 1.16E-10 1.15E-10 1.57567 1.51E-10 1.53933 neuronal growth regulator 1 NEGR1 NM_173808 1.01E-07 3.36E-07 1.57744 1.56E-07 1.69212 low density lipoprotein receptor-related protein 10 LRP10 NM_014045 1.25E-10 2.26E-10 1.58861 6.32E-10 1.45392 paired box gene 6 (aniridia, keratitis) PAX6 NM_000280 /// NM_0016047.97E-10 4.83E-10 1.58944 6.65E-10 1.54629 Ras-like without CAAX 1 RIT1 NM_006912 1.65E-08 1.31E-08 1.59353 3.40E-07 1.18752 CDNA FLJ34585 fis, clone KIDNE2008758 ------1.24E-08 2.20E-09 1.59489 2.09E-08 1.30884 major vault protein MVP NM_005115 /// NM_0174584.78E-10 6.93E-10 1.59512 4.10E-10 1.66894 tripartite motif-containing 22 TRIM22 NM_006074 9.03E-12 5.10E-10 1.60181 2.79E-10 1.68738 insulin-like growth factor binding protein 4 IGFBP4 NM_001552 4.87E-10 1.82E-09 1.60628 3.18E-09 1.52996 inter-alpha (globulin) inhibitor H3 ITIH3 NM_002217 2.29E-08 9.79E-09 1.60879 2.64E-08 1.47323 metastasis associated lung adenocarcinoma transcript 1 (non-coding RNA) MALAT1 --- 1.27E-11 4.54E-12 1.61195 1.03E-11 1.50393 myosin XVB pseudogene MYO15B XM_496245 /// XR_0002221.64E-10 9.25E-11 1.61585 1.22E-10 1.57857 N-myc downstream regulated gene 1 NDRG1 NM_006096 1.24E-09 4.56E-10 1.61912 7.15E-10 1.55754 cytoglobin CYGB NM_134268 1.93E-15 1.05E-11 1.62096 2.01E-12 1.86405 RAB31, member RAS oncogene family RAB31 NM_006868 4.70E-13 4.95E-11 1.62549 1.60E-10 1.47044 alpha-2-glycoprotein 1, zinc AZGP1 NM_001185 1.87E-10 2.23E-10 1.62579 3.68E-11 1.89637 protocadherin LKC PCLKC NM_017675 9.47E-09 6.11E-09 1.62815 2.69E-08 1.42826 Nuclear factor I/B NFIB NM_005596 8.39E-08 2.02E-07 1.63247 3.03E-07 1.5728 netrin 4 NTN4 NM_021229 1.34E-12 2.41E-11 1.63551 1.81E-11 1.676 chromosome 14 open reading frame 78 C14orf78 XM_290629 2.45E-08 2.41E-08 1.64653 2.19E-08 1.66055 Ras association (RalGDS/AF-6) domain family 2 RASSF2 NM_014737 /// NM_1707733.66E-08 /// NM_1707748.36E-08 1.65041 1.56E-07 1.55978 Transcribed locus ------6.12E-08 2.37E-08 1.65041 5.24E-08 1.53721 Hypothetical protein FLJ14681 FLJ14681 NM_032824 3.78E-12 1.56E-11 1.66291 3.30E-12 1.89671 yippee-like 5 (Drosophila) YPEL5 NM_016061 2.59E-11 1.84E-11 1.66398 1.67E-11 1.67724 ELOVL family member 7, elongation of long chain fatty acids (yeast) ELOVL7 NM_024930 9.41E-12 2.94E-11 1.66719 2.86E-12 2.03129 meteorin, glial cell differentiation regulator-like METRNL NM_001004431 1.41E-08 4.31E-09 1.67078 1.87E-08 1.46818 ADP-ribosylation factor-like 7 ARL7 NM_005737 9.61E-05 3.11E-05 1.67556 4.33E-05 1.61824 microtubule-associated protein 1B MAP1B NM_005909 /// NM_0320101.09E-06 1.48E-07 1.677 3.54E-06 1.24408 hypothetical protein FLJ22313 FLJ22313 NM_022373 6.43E-10 2.26E-09 1.69159 3.79E-09 1.61735 homeodomain interacting protein kinase 3 HIPK3 NM_005734 1.59E-07 4.97E-07 1.69674 6.65E-07 1.65112 zinc finger protein 226 ZNF226 NM_001032372 /// NM_0010323732.05E-07 /// NM_0010323743.61E-07 /// NM_0010323751.69689 /// NM_0159194.51E-07 1.66257 hypothetical gene CG018 CG018 NM_052818 2.13E-08 6.86E-09 1.69815 1.32E-07 1.30387 tripartite motif-containing 2 TRIM2 NM_015271 1.37E-08 1.20E-08 1.70078 5.33E-08 1.48972 SAM domain containing 1 LOC389432 NM_001030060 6.47E-09 3.16E-09 1.71082 9.49E-09 1.5534 nidogen 1 NID1 NM_002508 7.17E-12 1.71E-11 1.71149 1.79E-12 2.07152 mucin 15 MUC15 NM_145650 2.37E-06 1.32E-06 1.71702 8.00E-07 1.80069 p300/CBP-associated factor PCAF NM_003884 3.98E-08 1.77E-08 1.72486 2.19E-08 1.69212 sodium channel, voltage-gated, type IX, alpha SCN9A NM_002977 1.16E-10 6.96E-11 1.73061 4.71E-11 1.78937 sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) SGCB NM_000232 1.58E-11 3.62E-11 1.73127 9.79E-11 1.59033 nuclear factor I/B NFIB NM_005596 6.85E-09 1.23E-08 1.73912 3.60E-08 1.58035 synaptogyrin 3 SYNGR3 NM_004209 7.11E-09 1.46E-07 1.73957 1.03E-07 1.79529 interferon, alpha-inducible protein 27 IFI27 NM_005532 1.05E-11 7.68E-12 1.74636 6.31E-12 1.77564 reversion-inducing-cysteine-rich protein with kazal motifs RECK NM_021111 2.17E-07 3.14E-08 1.75222 5.15E-07 1.35798 hypothetical protein LOC51315 LOC51315 NM_016618 1.53E-09 1.50E-08 1.7688 5.08E-09 1.94538 nuclear receptor subfamily 1, group D, member 2 NR1D2 NM_005126 1.66E-09 6.55E-10 1.76979 7.42E-10 1.75097 myosin XVB pseudogene MYO15B XM_496245 /// XR_0002221.92E-08 5.29E-09 1.77596 1.55E-08 1.61577 nexilin (F actin binding protein) NEXN NM_144573 4.88E-09 3.14E-09 1.77732 1.94E-09 1.85396 myotubularin related protein 11 MTMR11 NM_006697 /// NM_1818739.56E-12 4.42E-12 1.78745 7.34E-12 1.71226 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

RNA binding motif protein 24 RBM24 NM_153020 1.85E-11 4.95E-10 1.79043 1.34E-10 2.00256 Ras-like without CAAX 1 RIT1 NM_006912 7.99E-11 3.48E-11 1.7911 1.07E-10 1.62751 modulator of apoptosis 1 MOAP1 NM_022151 1.57E-11 1.14E-11 1.79742 4.75E-12 1.93575 myosin XVB pseudogene MYO15B XM_496245 /// XR_0002221.18E-11 5.25E-12 1.80329 5.98E-12 1.78345 S100 calcium binding protein A6 (calcyclin) S100A6 NM_014624 1.18E-12 9.99E-13 1.81263 5.12E-13 1.9175 solute carrier family 44, member 1 SLC44A1 NM_022109 /// NM_0805464.49E-11 6.02E-11 1.81337 3.90E-11 1.88182 factor II () receptor F2R NM_001992 1.60E-11 2.07E-10 1.82636 1.02E-11 2.35992 complement component 5 C5 NM_001735 1.77E-10 3.70E-10 1.82723 4.09E-10 1.81178 arrestin domain containing 3 ARRDC3 NM_020801 8.75E-09 1.38E-09 1.83145 5.16E-08 1.33129 cyclin G2 CCNG2 NM_004354 3.29E-14 3.35E-14 1.83586 8.32E-14 1.70107 melanoma cell adhesion molecule MCAM NM_006500 4.19E-11 4.54E-11 1.84633 3.11E-11 1.90667 tubulin, beta polypeptide paralog TUBB-PARALOG NM_178012 1.84E-09 1.77E-09 1.85099 8.15E-10 1.9796 sushi domain containing 2 SUSD2 NM_019601 4.32E-11 1.72E-11 1.85652 2.00E-11 1.83297 Transcribed locus ------2.50E-08 2.00E-07 1.8661 4.30E-09 2.62919 chromosome 8 open reading frame 1 C8orf1 NM_004337 6.60E-14 1.56E-13 1.87107 3.78E-14 2.108 p8 protein (candidate of metastasis 1) P8 NM_012385 7.51E-12 4.69E-12 1.87327 5.14E-12 1.85865 leukocyte-associated Ig-like receptor 2 LAIR2 NM_002288 /// NM_0212702.13E-12 8.75E-13 1.88167 1.05E-12 1.85305 FLJ20160 protein FLJ20160 NM_017694 2.76E-06 2.09E-06 1.8859 1.57E-06 1.93768 adult retina protein LOC153222 NM_153607 2.10E-05 2.10E-06 1.89464 0.000496506 1.05672 cytochrome P450, family 3, subfamily A, polypeptide 5 CYP3A5 NM_000777 1.09E-12 4.63E-11 1.89632 3.66E-11 1.93469 MRNA; cDNA DKFZp586B211 (from clone DKFZp586B211) ------4.70E-07 4.10E-07 1.89691 1.90E-06 1.64162 collagen, type IV, alpha 5 (Alport syndrome) COL4A5 NM_000495 /// NM_0333805.30E-16 /// NM_0333817.07E-15 1.90107 1.26E-14 1.81076 cytidine deaminase CDA NM_001785 3.34E-13 7.28E-13 1.91113 1.57E-12 1.79072 tropomyosin 2 (beta) TPM2 NM_003289 /// NM_2136741.16E-08 7.51E-09 1.92348 6.26E-09 1.95448 ras homolog gene family, member Q /// similar to ARHQ protein RHOQ /// LOC284988NM_012249 /// XM_2094296.02E-10 9.49E-09 1.93559 1.59E-08 1.84952 Cyclin G2 CCNG2 NM_004354 4.36E-11 4.53E-11 1.94579 6.16E-11 1.89514 Eukaryotic translation initiation factor 2C, 4 EIF2C4 NM_017629 7.74E-14 9.23E-13 1.95965 1.95E-12 1.83984 aquaporin 3 AQP3 NM_004925 7.77E-12 5.78E-12 1.97257 1.85E-11 1.78752 melanoma cell adhesion molecule MCAM NM_006500 3.18E-14 3.65E-14 1.97962 2.60E-14 2.03678 chromosome 10 open reading frame 10 C10orf10 NM_007021 1.28E-07 5.35E-08 2.00371 1.17E-07 1.86638 Homeodomain interacting protein kinase 3 HIPK3 NM_005734 6.65E-11 6.19E-11 2.00536 1.86E-11 2.2221 cyclin G2 CCNG2 NM_004354 7.53E-12 1.19E-11 2.06466 1.37E-11 2.04009 nexilin (F actin binding protein) NEXN NM_144573 1.15E-10 5.78E-11 2.0672 4.51E-11 2.11142 CD9 antigen (p24) CD9 NM_001769 2.89E-13 3.35E-13 2.07266 8.21E-14 2.33295 Nuclear factor I/B NFIB NM_005596 2.12E-10 1.45E-10 2.08388 1.45E-10 2.0829 Similar to hypothetical protein LOC231503 --- XM_496707 2.51E-08 8.15E-09 2.09064 1.62E-08 1.96763 MRNA; cDNA DKFZp586B211 (from clone DKFZp586B211) ------1.51E-08 2.99E-09 2.09364 4.79E-08 1.63842 chloride intracellular channel 3 CLIC3 NM_004669 7.52E-09 4.70E-09 2.09614 7.98E-09 2.00099 downregulated in ovarian cancer 1 DOC1 NM_014890 /// NM_1829091.96E-12 1.06E-12 2.1077 8.73E-13 2.14257 KIAA0746 protein KIAA0746 NM_015187 9.45E-11 1.77E-10 2.13476 1.08E-10 2.2271 cytochrome P450, family 3, subfamily A, polypeptide 5 CYP3A5 NM_000777 7.75E-15 8.95E-14 2.13918 9.63E-14 2.1261 Full-length cDNA clone CS0DJ001YJ05 of T cells (Jurkat cell line) Cot 10-normali ------1.14E-09 4.32E-09 2.14909 3.08E-09 2.21395 hypothetical protein FLJ39370 FLJ39370 NM_152400 1.19E-15 7.87E-15 2.17037 3.76E-16 2.7998 TIMP metallopeptidase inhibitor 2 TIMP2 NM_003255 4.16E-12 1.41E-11 2.18631 4.20E-11 1.99268 TIMP metallopeptidase inhibitor 2 TIMP2 NM_003255 2.32E-13 4.20E-13 2.19094 6.39E-13 2.11477 keratin 23 (histone deacetylase inducible) KRT23 NM_015515 /// NM_1732135.87E-12 5.97E-12 2.20397 2.03E-12 2.41481 chondroitin beta1,4 N-acetylgalactosaminyltransferase ChGn NM_018371 1.56E-09 8.19E-10 2.2216 5.61E-10 2.29548 synaptotagmin-like 2 SYTL2 NM_032379 /// NM_0329437.10E-08 /// NM_2069272.49E-08 /// NM_206928 ///2.22936 NM_206929 /// 3.70E-08NM_206930 2.15173 solute carrier family 4, sodium bicarbonate transporter-like, member 11 SLC4A11 NM_032034 5.89E-12 3.04E-12 2.24288 2.24E-12 2.3017 spondin 2, extracellular matrix protein SPON2 NM_012445 5.68E-11 2.96E-11 2.25367 2.49E-11 2.28706 CDNA clone IMAGE:5531727 ------7.55E-12 1.12E-11 2.25492 3.85E-12 2.46933 cytochrome P450, family 3, subfamily A, polypeptide 5 CYP3A5 NM_000777 3.99E-14 2.03E-12 2.28742 6.90E-12 2.06242 KIAA0746 protein KIAA0746 NM_015187 1.07E-10 1.70E-10 2.30366 7.53E-11 2.46948 ADP-ribosylation factor-like 7 ARL7 NM_005737 2.41E-09 9.19E-10 2.31313 1.31E-09 2.24279 RAS, dexamethasone-induced 1 RASD1 NM_016084 4.43E-14 2.48E-14 2.35424 2.88E-14 2.32427 CDNA clone IMAGE:5531727 ------3.38E-13 7.31E-13 2.39716 6.10E-13 2.43426 FLJ34306 protein FLJ34306 NM_199340 2.53E-09 1.59E-09 2.43393 5.90E-09 2.17094 kelch-like 24 (Drosophila) KLHL24 NM_017644 4.25E-12 2.98E-12 2.44349 3.72E-12 2.39839 downregulated in ovarian cancer 1 DOC1 NM_014890 /// NM_1829092.38E-13 1.52E-13 2.45881 8.73E-14 2.57571 lysyl oxidase-like 2 LOXL2 NM_002318 5.62E-12 3.47E-12 2.46307 3.88E-12 2.43964 Supplemental Table 1: Genes that show altered expression in HepG2 cells in the presence of exogenously added let-7

guanine nucleotide binding protein (G protein), alpha inhibiting activity polype GNAI1 NM_002069 5.59E-12 3.23E-12 2.46435 5.51E-12 2.35526 solute carrier family 40 (iron-regulated transporter), member 1 SLC40A1 NM_014585 1.25E-14 9.41E-15 2.50136 3.74E-14 2.22831 S100 calcium binding protein A2 S100A2 NM_005978 1.77E-14 3.03E-14 2.53289 2.30E-14 2.59218 guanine nucleotide binding protein (G protein), alpha inhibiting activity polype GNAI1 NM_002069 2.76E-11 3.79E-11 2.54227 3.64E-11 2.55072 tubulin, alpha 3 TUBA3 NM_006009 4.64E-14 2.07E-14 2.68247 4.16E-14 2.5296 aquaporin 3 AQP3 NM_004925 2.37E-14 4.77E-14 2.69376 2.80E-13 2.32149 matrix metallopeptidase 3 (stromelysin 1, progelatinase) MMP3 NM_002422 2.05E-14 1.89E-13 2.70638 1.12E-13 2.82748 Transcribed locus ------6.77E-10 5.30E-10 2.71501 1.84E-10 2.97383 solute carrier family 4, sodium bicarbonate transporter-like, member 11 SLC4A11 NM_032034 1.19E-12 8.73E-13 2.81055 3.34E-13 3.04711 synaptotagmin-like 2 SYTL2 NM_032379 /// NM_0329432.44E-10 /// NM_2069271.25E-10 /// NM_206928 ///2.90994 NM_206929 /// 8.83E-11NM_206930 2.99832 keratin associated protein 3-1 KRTAP3-1 NM_031958 2.35E-13 3.36E-13 2.99409 1.18E-13 3.26855 ADP-ribosylation factor-like 7 ARL7 NM_005737 8.00E-14 2.67E-14 3.07174 7.42E-14 2.81937 lymphocyte antigen 96 LY96 NM_015364 8.82E-13 7.52E-13 3.59966 5.80E-13 3.67918 keratin 20 KRT20 NM_019010 5.53E-15 4.67E-15 3.83038 4.00E-15 3.88075 aldehyde dehydrogenase 3 family, memberA1 ALDH3A1 NM_000691 1.58E-10 1.21E-10 3.93671 8.96E-11 4.0387 Supplemental Table 2: Genes that show altered expression in A549 cells in the presence of exogenously added let-7

Gene Title Gene Symbol RefSeq Transcriptp-value(TREA ID p-vTMENTalue(Let7bS) -Log2 negativ Ratioe control1)(Let7b p-- negativvalue(Let7be control1) -Log2 negativ Ratioe control2)(Let7b - negative control2) haloacid dehalogenase-like hydrolase domain containing 1A HDHD1A NM_012080 1.03E-11 1.83E-11 -2.71347 5.89E-12 -3.07909 transmembrane 4 L six family member 20 TM4SF20 NM_024795 1.59E-08 8.87E-09 -2.59749 2.87E-08 -2.27239 high mobility group AT-hook 2 /// high mobility group AT-hook 2 HMGA2 NM_001015886 /// 4.08E-10NM_003483 /// NM_0034846.53E-10 -2.11164 2.48E-10 -2.35366 dopa decarboxylase (aromatic L-amino acid decarboxylase) DDC NM_000790 2.55E-11 2.94E-11 -2.0939 1.99E-11 -2.18776 lin-28 homolog B (C. elegans) LIN28B NM_001004317 6.77E-12 1.34E-10 -2.05419 1.92E-12 -3.30099 fibrinogen beta chain FGB NM_005141 1.48E-07 4.92E-07 -2.03096 6.08E-08 -2.58588 chromosome 9 open reading frame 125 /// chromosome 9 open reading frameC9orf125 125 NM_032342 1.05E-07 1.18E-07 -1.97781 8.01E-08 -2.06729 fibrinogen beta chain FGB NM_005141 2.06E-09 5.34E-09 -1.9513 9.60E-10 -2.36822 Chromobox homolog 5 (HP1 alpha homolog, Drosophila) CBX5 NM_012117 4.31E-05 3.83E-05 -1.92991 3.55E-05 -1.94873 chromosome 6 open reading frame 176 C6orf176 XM_499048 4.55E-09 8.65E-09 -1.90363 2.47E-09 -2.1935 Dicer1, Dcr-1 homolog (Drosophila) DICER1 NM_030621 /// NM_1774388.60E-09 2.16E-08 -1.8869 4.04E-09 -2.2821 ERO1-like (S. cerevisiae) ERO1L NM_014584 8.95E-13 1.16E-12 -1.87685 6.38E-13 -2.00625 heterogeneous nuclear ribonucleoprotein C (C1/C2) HNRPC NM_004500 /// NM_0313141.13E-09 9.59E-10 -1.86869 1.17E-09 -1.82754 Dicer1, Dcr-1 homolog (Drosophila) DICER1 NM_030621 /// NM_1774388.67E-09 2.80E-08 -1.83404 3.66E-09 -2.31021 CDNA FLJ26015 fis, clone MPC03596 ------1.22E-08 1.39E-08 -1.82122 9.34E-09 -1.90555 zinc finger, CSL-type containing 2 ZCSL2 NM_206831 9.19E-10 1.79E-09 -1.78074 4.95E-10 -2.05834 CDC20-like protein FLJ37927 NM_152623 0.000276561 8.32E-05 -1.71872 0.00222672 -1.07471 RNA pseudouridylate synthase domain containing 3 RPUSD3 NM_173659 1.28E-10 1.60E-10 -1.68298 9.29E-11 -1.78871 potassium inwardly-rectifying channel, subfamily J, member 16 KCNJ16 NM_018658 /// NM_1707413.28E-08 /// NM_1707421.49E-07 -1.6595 1.22E-08 -2.20965 neuroblastoma RAS viral (v-ras) oncogene homolog NRAS NM_002524 9.78E-12 1.13E-11 -1.64907 7.64E-12 -1.72212 hypothetical LOC83640 MGC2560 NM_031452 1.96E-10 1.88E-10 -1.6382 1.81E-10 -1.64519 Solute carrier family 4, sodium bicarbonate cotransporter, member 5 SLC4A5 NM_021196 /// NM_0333232.33E-08 /// NM_1334786.71E-09 /// NM_133479-1.61994 3.73E-07 -1.02222 jub, ajuba homolog (Xenopus laevis) JUB NM_032876 /// NM_1980861.16E-09 6.76E-10 -1.58732 1.97E-09 -1.4072 nucleoporin 98kDa NUP98 NM_005387 /// NM_0163202.99E-10 /// NM_1391315.58E-10 /// NM_139132-1.58055 1.66E-10 -1.81079 hyaluronan-mediated motility receptor (RHAMM) HMMR NM_012484 /// NM_0124853.10E-10 6.22E-10 -1.57449 1.65E-10 -1.82722 organic solute transporter beta OSTbeta NM_178859 7.94E-08 2.57E-07 -1.572 3.31E-08 -1.98966 CDNA clone IMAGE:5301910 ------4.33E-08 4.04E-08 -1.56785 3.96E-08 -1.57175 Protein kinase, cAMP-dependent, regulatory, type II, alpha PRKAR2A NM_004157 1.27E-12 1.59E-12 -1.56136 9.36E-13 -1.65585 Dicer1, Dcr-1 homolog (Drosophila) DICER1 NM_030621 /// NM_1774382.26E-07 1.18E-06 -1.56028 8.02E-08 -2.1326 cell division cycle 34 CDC34 NM_004359 1.54E-08 2.01E-08 -1.55002 1.07E-08 -1.66521 Protein kinase, cAMP-dependent, regulatory, type II, alpha PRKAR2A NM_004157 6.18E-10 5.66E-10 -1.5425 5.91E-10 -1.53501 Galactosidase, beta 1 GLB1 NM_000404 1.01E-09 3.01E-09 -1.53912 4.44E-10 -1.90889 serpin peptidase inhibitor, clade B (ovalbumin), member 9 SERPINB9 NM_004155 3.83E-11 2.62E-10 -1.51528 1.30E-11 -2.12096 jub, ajuba homolog (Xenopus laevis) JUB NM_032876 /// NM_1980867.20E-09 4.97E-09 -1.50851 9.45E-09 -1.40275 fibrinogen alpha chain FGA NM_000508 /// NM_0218714.00E-11 2.29E-10 -1.50784 1.42E-11 -2.05873 dopa decarboxylase (aromatic L-amino acid decarboxylase) DDC NM_000790 8.53E-08 8.82E-08 -1.50618 7.03E-08 -1.5458 Cyclin-dependent kinase 8 CDK8 NM_001260 2.30E-09 6.36E-09 -1.50589 1.04E-09 -1.84763 vestigial-like 3 VGL-3 NM_016206 1.67E-08 1.60E-07 -1.50045 5.22E-09 -2.21808 solute carrier family 5 (sodium-dependent vitamin transporter), member 6 SLC5A6 NM_021095 2.09E-08 2.75E-08 -1.49987 1.43E-08 -1.61586 Full-length cDNA clone CS0DL007YI24 of B cells (Ramos cell line) Cot 25-normaliz------1.88E-10 7.58E-10 -1.49506 7.41E-11 -1.94048 hypothetical protein MGC13204 MGC13204 NM_031465 1.86E-10 2.29E-10 -1.49167 1.36E-10 -1.58091 transforming growth factor, beta receptor I (activin A receptor type II-like kin TGFBR1 NM_004612 1.37E-08 5.36E-09 -1.48274 5.38E-08 -1.14059 hypothetical protein MGC13204 MGC13204 NM_031465 1.54E-08 2.25E-08 -1.47902 9.77E-09 -1.62653 Transcribed locus ------5.37E-08 1.80E-07 -1.47802 2.21E-08 -1.88005 E2F transcription factor 5, p130-binding E2F5 NM_001951 1.70E-09 2.57E-09 -1.46603 1.07E-09 -1.61854 leptin receptor overlapping transcript-like 1 LEPROTL1 NM_015344 5.10E-10 3.81E-10 -1.46462 6.14E-10 -1.38822 CCAAT/enhancer binding protein (C/EBP), delta CEBPD NM_005195 3.57E-09 1.13E-08 -1.44686 1.52E-09 -1.81514 adenosine deaminase ADA NM_000022 1.71E-08 1.16E-08 -1.43997 2.28E-08 -1.33399 hyaluronan-mediated motility receptor (RHAMM) HMMR NM_012484 /// NM_0124852.77E-07 4.78E-07 -1.43976 1.55E-07 -1.64081 aurora kinase B AURKB NM_004217 1.37E-08 3.68E-08 -1.43399 6.20E-09 -1.75581 solute carrier family 7 (cationic amino acid transporter, y+ system), member 5SLC7A5 NM_003486 2.24E-08 1.20E-08 -1.4316 4.30E-08 -1.23769 proteasome (prosome, macropain) activator subunit 4 PSME4 NM_014614 8.01E-06 1.39E-05 -1.42677 4.23E-06 -1.65002 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferGALNT2ase NM_004481 5.64E-10 1.85E-09 -1.42298 2.38E-10 -1.79155 MHC class I polypeptide-related sequence B MICB NM_005931 1.42E-09 1.28E-08 -1.42164 4.50E-10 -2.07445 dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferDPAGT1a NM_001382 /// NM_2033161.64E-09 1.22E-09 -1.39882 1.97E-09 -1.32502 hypothetical protein MGC23909 MGC23909 NM_174909 5.21E-09 1.47E-08 -1.39562 2.33E-09 -1.71884 Hypothetical protein LOC285513 LOC285513 NM_198281 1.22E-07 1.88E-07 -1.38828 7.34E-08 -1.5469 Supplemental Table 2: Genes that show altered expression in A549 cells in the presence of exogenously added let-7

aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase AASDHPPT NM_015423 4.68E-09 3.91E-09 -1.38633 4.89E-09 -1.35157 vacuolar protein sorting 54 (yeast) VPS54 NM_001005739 /// 4.44E-10NM_016516 7.55E-10 -1.38335 2.60E-10 -1.5593 chromosome 10 open reading frame 9 C10orf9 NM_145012 /// NM_1816982.46E-05 1.35E-05 -1.38129 3.79E-05 -1.21371 guanine nucleotide binding protein (G protein), gamma 5 GNG5 NM_005274 1.03E-11 1.01E-11 -1.38079 9.29E-12 -1.39324 TIGA1 TIGA1 NM_053000 2.83E-05 1.29E-05 -1.37205 6.22E-05 -1.1248 adenosine deaminase ADA NM_000022 5.08E-10 3.48E-10 -1.36609 6.82E-10 -1.26669 Cyclin-dependent kinase 8 CDK8 NM_001260 5.94E-07 1.73E-06 -1.36572 2.54E-07 -1.70977 similar to RIKEN cDNA 1200014N16 gene MGC14289 NM_080660 2.73E-09 1.17E-08 -1.36354 1.05E-09 -1.79019 hypothetical protein BC014072 LOC116238 NM_138463 3.41E-11 1.32E-11 -1.36343 1.45E-10 -1.04253 Chromosome 3 open reading frame 17 C3orf17 NM_001025072 /// 5.47E-08NM_001025073 4.64E-08/// NM_015412 -1.35438 5.50E-08 -1.32829 synaptosomal-associated protein, 23kDa SNAP23 NM_003825 /// NM_1307981.40E-10 4.68E-11 -1.35369 9.82E-10 -0.962362 ADP-ribosylation-like factor 6 interacting protein 6 ARL6IP6 NM_152522 6.06E-08 1.61E-07 -1.35172 2.75E-08 -1.65588 RNA terminal phosphate cyclase domain 1 RTCD1 NM_003729 7.54E-11 1.14E-10 -1.35139 4.78E-11 -1.48964 Chromosome 9 open reading frame 3 NM_032823 0.000832262 0.000350228 -1.34965 0.00168139 -1.0712 serine/threonine kinase 6 STK6 NM_003600 /// NM_1984332.24E-11 /// NM_1984344.34E-11 /// NM_198435-1.34628 /// NM_1984361.22E-11 /// NM_198437-1.55077 progesterone receptor membrane component 1 PGRMC1 NM_006667 6.88E-11 6.36E-11 -1.34004 6.57E-11 -1.33537 reticulon 4 receptor-like 2 RTN4RL2 NM_178570 0.000741109 0.000343294 -1.32286 0.00123434 -1.09811 ERO1-like (S. cerevisiae) ERO1L NM_014584 4.27E-11 5.71E-11 -1.32073 2.96E-11 -1.42113 progesterone receptor membrane component 1 PGRMC1 NM_006667 2.55E-10 2.79E-10 -1.3082 2.07E-10 -1.35254 fibrinogen alpha chain FGA NM_000508 /// NM_0218717.64E-08 5.42E-07 -1.30642 2.52E-08 -1.86061 reticulon 4 interacting protein 1 /// reticulon 4 interacting protein 1 RTN4IP1 NM_032730 1.29E-07 1.72E-07 -1.30243 8.64E-08 -1.40952 golgi transport 1 homolog B (S. cerevisiae) GOLT1B NM_016072 5.24E-09 5.79E-09 -1.30082 4.17E-09 -1.3502 chromosome 13 open reading frame 1 C13orf1 NM_020456 2.21E-08 5.24E-08 -1.29949 1.06E-08 -1.55841 hypothetical protein LOC285943 LOC285943 --- 5.72E-08 6.43E-08 -1.29877 4.40E-08 -1.35636 chromosome 14 open reading frame 2 C14orf2 NM_004894 2.82E-10 2.48E-10 -1.29453 2.83E-10 -1.27526 pleiomorphic adenoma gene-like 2 PLAGL2 NM_002657 1.57E-10 4.78E-10 -1.29304 6.88E-11 -1.60694 galactosylceramidase (Krabbe disease) GALC NM_000153 4.09E-07 2.60E-07 -1.28963 5.74E-07 -1.17572 transketolase (Wernicke-Korsakoff syndrome) TKT NM_001064 9.63E-07 7.70E-07 -1.28645 9.91E-07 -1.24887 LSM6 homolog, U6 small nuclear RNA associated (S. cerevisiae) LSM6 NM_007080 3.71E-09 3.75E-09 -1.2864 3.20E-09 -1.3097 hypothetical protein FLJ90709 FLJ90709 NM_173514 6.28E-09 9.37E-09 -1.28623 3.96E-09 -1.41784 hypothetical protein FLJ10700 FLJ10700 NM_018182 2.00E-08 6.78E-08 -1.28589 8.27E-09 -1.63434 signal peptidase complex subunit 3 homolog (S. cerevisiae) SPCS3 NM_021928 2.11E-08 3.25E-08 -1.28302 1.29E-08 -1.4247 leucine-rich repeat-containing G protein-coupled receptor 4 LGR4 NM_018490 2.36E-07 2.34E-07 -1.28092 1.99E-07 -1.30498 nei endonuclease VIII-like 3 (E. coli) NEIL3 NM_018248 1.22E-10 1.27E-10 -1.27749 1.03E-10 -1.30745 cysteine-rich, angiogenic inducer, 61 CYR61 NM_001554 9.41E-08 5.97E-07 -1.26767 3.19E-08 -1.77785 Leptin receptor LEPR NM_001003679 /// 1.07E-07NM_001003680 4.08E-07/// NM_002303 -1.26663 4.19E-08 -1.64684 poly(A) polymerase alpha PAPOLA NM_032632 4.24E-09 3.41E-09 -1.26632 4.63E-09 -1.22322 chromosome 20 open reading frame 177 C20orf177 NM_022106 1.10E-09 1.25E-09 -1.2644 8.66E-10 -1.31756 non-metastatic cells 4, protein expressed in NME4 NM_005009 4.51E-09 3.69E-09 -1.26162 4.83E-09 -1.22375 solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 24SLC25A24 NM_013386 /// NM_2136512.41E-08 2.00E-08 -1.26098 2.49E-08 -1.22988 zinc finger, CSL-type containing 2 ZCSL2 NM_206831 9.19E-12 1.24E-11 -1.25851 6.32E-12 -1.35726 CTP synthase II CTPS2 NM_019857 /// NM_1758595.38E-10 3.51E-10 -1.25646 7.77E-10 -1.14918 transferrin receptor (p90, CD71) TFRC NM_003234 0.000328157 0.000376042 -1.25203 0.000199053 -1.36693 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminideSIAT7B NM_006456 1.79E-10 1.80E-10 -1.24645 1.57E-10 -1.26595 chromosome 10 open reading frame 9 C10orf9 NM_145012 /// NM_1816981.31E-09 1.02E-09 -1.24411 1.50E-09 -1.19138 IGF-II mRNA-binding protein 1 IMP-1 NM_006546 1.89E-08 7.89E-08 -1.24077 7.24E-09 -1.62892 fibrinogen gamma chain FGG NM_000509 /// NM_0218701.79E-07 2.35E-07 -1.23702 1.21E-07 -1.33588 Fidgetin FIGN NM_018086 1.19E-06 7.58E-06 -1.23464 3.97E-07 -1.7532 adaptor-related protein complex 1, sigma 1 subunit AP1S1 NM_001283 /// NM_0570892.20E-08 1.86E-08 -1.23081 2.25E-08 -1.20435 H2B histone family, member S H2BFS NM_017445 5.32E-07 4.89E-07 -1.22756 4.78E-07 -1.231 vacuolar protein sorting 54 (yeast) VPS54 NM_001005739 /// 1.74E-12NM_016516 4.68E-12 -1.22588 8.08E-13 -1.491 hypothetical protein LOC283464 LOC283464 XM_290597 1.26E-08 9.08E-07 -1.22287 3.29E-09 -2.33088 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferGALNT2ase NM_004481 8.35E-09 3.95E-08 -1.21057 3.09E-09 -1.61668 UDP-galactose-4-epimerase GALE NM_000403 /// NM_0010082167.80E-08 8.73E-08 -1.20636 6.00E-08 -1.25936 hypothetical protein MGC2408 MGC2408 NM_032331 2.08E-08 3.51E-08 -1.20555 1.21E-08 -1.36129 chromosome 6 open reading frame 211 C6orf211 NM_024573 2.15E-07 1.60E-07 -1.20443 2.48E-07 -1.14497 neurolysin (metallopeptidase M3 family) NLN NM_020726 8.43E-10 9.16E-10 -1.20272 6.85E-10 -1.24258 non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase) NME6 NM_005793 2.14E-08 8.23E-08 -1.20087 8.44E-09 -1.55685 Supplemental Table 2: Genes that show altered expression in A549 cells in the presence of exogenously added let-7

Kell blood group precursor (McLeod phenotype) XK NM_021083 4.28E-06 4.96E-06 -1.19909 3.01E-06 -1.27334 solute carrier family 35, member D2 SLC35D2 NM_007001 2.27E-07 1.93E-06 -1.19383 7.18E-08 -1.75184 reticulon 4 interacting protein 1 RTN4IP1 NM_032730 5.73E-07 8.89E-07 -1.19378 3.38E-07 -1.33666 zinc finger, CCHC domain containing 9 ZCCHC9 NM_032280 7.24E-08 2.13E-07 -1.18979 3.14E-08 -1.48207 homeo box A1 HOXA1 NM_005522 /// NM_1536207.78E-11 2.87E-10 -1.1874 3.16E-11 -1.52048 mitochondrial tumor suppressor 1 MTUS1 NM_001001924 /// 1.37E-06NM_001001925 6.54E-07/// NM_001001927-1.18702 /// NM_0010019313.02E-06 /// NM_020749-0.990228 IGF-II mRNA-binding protein 2 IMP-2 NM_001007225 /// 3.87E-08NM_006548 1.24E-07 -1.18386 1.63E-08 -1.49407 stress-associated endoplasmic reticulum protein 1 SERP1 NM_014445 5.78E-10 7.67E-10 -1.18256 4.00E-10 -1.27228 kynureninase (L-kynurenine hydrolase) KYNU NM_001032998 /// 1.27E-06NM_003937 3.19E-06 -1.17578 5.76E-07 -1.43972 ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C, isoform 1 ATP6V1C1 NM_001007254 /// 5.41E-09NM_001695 5.52E-09 -1.17569 4.62E-09 -1.19946 chromosome 1 open reading frame 25 C1orf25 NM_030934 1.99E-09 1.24E-09 -1.16502 3.02E-09 -1.0543 MRNA; cDNA DKFZp564C0762 (from clone DKFZp564C0762) ------7.39E-08 1.16E-07 -1.16225 4.44E-08 -1.29784 mediator of RNA polymerase II transcription, subunit 6 homolog (yeast) MED6 NM_005466 1.17E-07 1.63E-07 -1.15895 7.54E-08 -1.26681 gem (nuclear organelle) associated protein 7 GEMIN7 NM_001007269 /// 6.45E-11NM_001007270 2.46E-10/// NM_024707 -1.15517 2.59E-11 -1.4859 Cold shock domain containing E1, RNA-binding UNR NM_001007553 /// 3.87E-10NM_007158 4.71E-10 -1.15067 2.87E-10 -1.21668 solute carrier family 35, member D2 SLC35D2 NM_007001 3.53E-09 1.74E-08 -1.14982 1.29E-09 -1.54315 Phosphodiesterase 3A, cGMP-inhibited PDE3A NM_000921 2.01E-07 3.59E-07 -1.14647 1.11E-07 -1.31399 cyclin A2 CCNA2 NM_001237 1.01E-10 3.55E-10 -1.14528 4.16E-11 -1.45583 histone 1, H2bk HIST1H2BK NM_080593 7.07E-10 1.16E-09 -1.13895 4.24E-10 -1.27505 KIAA0507 KIAA0507 --- 2.63E-07 6.83E-07 -1.13835 1.19E-07 -1.3944 activin A receptor, type IB ACVR1B NM_004302 /// NM_0203272.37E-11 /// NM_0203281.63E-11 -1.13733 3.20E-11 -1.05484 cyclin-dependent kinase 8 CDK8 NM_001260 2.17E-09 9.41E-09 -1.13687 8.31E-10 -1.49507 60S ribosomal protein L6 (RPL6A) NAP1L1 NM_004537 /// NM_1392077.08E-06 6.95E-06 -1.13451 5.66E-06 -1.16305 CTP synthase CTPS NM_001905 1.73E-07 1.37E-07 -1.13418 1.84E-07 -1.09659 Vacuolar protein sorting 33A (yeast) VPS33A NM_022916 6.56E-07 1.10E-06 -1.13375 3.68E-07 -1.28889 S100P binding protein Riken S100PBPR NM_001017406 /// 5.90E-09NM_022753 5.12E-09 -1.13314 5.91E-09 -1.1148 chromosome 6 open reading frame 120 C6orf120 NM_001029863 2.41E-09 5.22E-09 -1.13223 1.22E-09 -1.33386 p18 splicing regulatory protein P18SRP NM_173829 1.52E-05 7.99E-06 -1.1313 2.60E-05 -0.977402 Protein kinase, cAMP-dependent, regulatory, type II, alpha PRKAR2A NM_004157 9.27E-10 1.03E-09 -1.12918 7.39E-10 -1.17214 activator of S phase kinase ASK NM_006716 4.73E-07 6.27E-07 -1.12798 3.12E-07 -1.22352 adaptor-related protein complex 1, sigma 1 subunit AP1S1 NM_001283 /// NM_0570894.31E-07 2.54E-07 -1.1267 6.75E-07 -1.00543 dual specificity phosphatase 16 /// dual specificity phosphatase 16 DUSP16 NM_030640 6.19E-09 3.55E-08 -1.12525 2.17E-09 -1.54484 ATG10 autophagy related 10 homolog (S. cerevisiae) ATG10 NM_031482 8.82E-07 2.02E-06 -1.12119 4.18E-07 -1.3497 NMDA receptor regulated 1 NARG1 NM_057175 6.79E-06 1.45E-05 -1.11774 3.22E-06 -1.34211 geminin, DNA replication inhibitor GMNN NM_015895 4.55E-12 7.35E-12 -1.11628 2.77E-12 -1.24458 transmembrane protein 2 TMEM2 NM_013390 5.34E-10 3.73E-09 -1.11531 1.79E-10 -1.5691 gem (nuclear organelle) associated protein 7 GEMIN7 NM_001007269 /// 5.10E-10NM_001007270 1.54E-09/// NM_024707 -1.11492 2.23E-10 -1.38496 CDNA FLJ13202 fis, clone NT2RP3004503 ------2.06E-07 2.63E-07 -1.11223 1.41E-07 -1.19481 calcium activated nucleotidase 1 CANT1 NM_138793 2.40E-08 2.01E-08 -1.11026 2.47E-08 -1.08478 KIAA1287 KIAA1287 NM_020748 6.00E-09 6.08E-09 -1.10839 5.14E-09 -1.12979 Hypothetical LOC133993 --- XM_068632 1.20E-07 1.17E-07 -1.10452 1.04E-07 -1.11896 RNA binding protein with multiple splicing RBPMS NM_001008710 /// 2.90E-08NM_001008711 7.56E-08/// NM_001008712-1.10173 /// NM_0068671.33E-08 -1.34331 solute carrier family 25, member 32 /// solute carrier family 25, member 32 SLC25A32 NM_030780 1.16E-08 1.71E-08 -1.10093 7.39E-09 -1.21074 solute carrier family 25, member 13 (citrin) SLC25A13 NM_014251 2.08E-10 8.08E-10 -1.10079 8.29E-11 -1.42093 poly(A) binding protein, cytoplasmic 4 (inducible form) PABPC4 NM_003819 7.23E-09 1.03E-08 -1.10078 4.72E-09 -1.20198 chromosome 14 open reading frame 46 C14orf46 NM_001024674 3.56E-07 3.38E-07 -1.0999 3.12E-07 -1.11005 RIO kinase 2 (yeast) RIOK2 NM_018343 6.88E-07 2.06E-06 -1.09985 2.90E-07 -1.38479 general transcription factor II, i GTF2I NM_001518 /// NM_0329990.000450116 /// NM_0330000.000284957 /// NM_033001-1.09608 0.000476165 -1.01991 CD164 antigen, sialomucin CD164 NM_006016 4.37E-10 6.98E-10 -1.09443 2.66E-10 -1.2197 MRS2-like, magnesium homeostasis factor (S. cerevisiae) MRS2L NM_020662 1.62E-10 4.82E-10 -1.09357 7.14E-11 -1.35462 cysteine-rich, angiogenic inducer, 61 CYR61 NM_001554 3.89E-08 2.31E-07 -1.09299 1.34E-08 -1.51418 Transcribed locus ------5.97E-06 6.72E-06 -1.09212 4.26E-06 -1.15429 solute carrier family 17 (anion/sugar transporter), member 5 SLC17A5 NM_012434 7.10E-10 1.83E-09 -1.0907 3.32E-10 -1.32193 ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F ATP6V1F NM_004231 2.42E-11 2.16E-11 -1.0883 2.39E-11 -1.07605 PTX1 protein PTX1 NM_016570 6.68E-08 1.63E-07 -1.08811 3.15E-08 -1.3137 nucleosome assembly protein 1-like 1 NAP1L1 NM_004537 /// NM_1392076.53E-08 6.93E-08 -1.08745 5.27E-08 -1.1222 coagulation factor II (thrombin) receptor-like 2 F2RL2 NM_004101 1.30E-07 4.10E-06 -1.08213 3.52E-08 -1.8855 STARD3 N-terminal like STARD3NL NM_032016 3.59E-11 8.59E-11 -1.07988 1.75E-11 -1.29022 Supplemental Table 2: Genes that show altered expression in A549 cells in the presence of exogenously added let-7

ring finger protein 13 RNF13 NM_007282 /// NM_1833819.77E-10 /// NM_1833828.46E-10 /// NM_183383-1.07731 /// NM_1833849.90E-10 -1.05849 phosphatidylinositol glycan, class A (paroxysmal nocturnal hemoglobinuria) ///PIGA p NM_002641 /// NM_0204721.72E-06 /// NM_0204734.03E-06 -1.0759 7.97E-07 -1.30454 vacuolar protein sorting 54 (yeast) VPS54 NM_001005739 /// 8.78E-10NM_016516 2.24E-09 -1.07138 4.13E-10 -1.29599 mediator of RNA polymerase II transcription, subunit 6 homolog (yeast) MED6 NM_005466 8.74E-09 1.86E-08 -1.06693 4.45E-09 -1.25475 pleiomorphic adenoma gene-like 1 PLAGL1 NM_002656 /// NM_0067182.23E-06 4.39E-06 -1.06676 1.12E-06 -1.25491 de-etiolated homolog 1 (Arabidopsis) DET1 NM_017996 4.94E-08 3.04E-08 -1.06566 7.52E-08 -0.960726 galactosidase, beta 1 GLB1 NM_000404 8.25E-10 5.83E-10 -1.06425 1.06E-09 -0.99459 Transcribed locus ------0.000167776 0.000461116 -1.06296 6.61E-05 -1.38232 apobec-1 complementation factor ACF NM_014576 /// NM_1389325.15E-07 /// NM_1389331.40E-06 -1.0592 2.27E-07 -1.31005 serine/threonine kinase 6 STK6 NM_003600 /// NM_1984337.57E-10 /// NM_1984342.75E-09 /// NM_198435-1.05839 /// NM_1984363.08E-10 /// NM_198437-1.35383 interleukin 11 IL11 NM_000641 9.77E-09 1.30E-06 -1.05836 2.55E-09 -2.1662 coproporphyrinogen oxidase CPOX NM_000097 8.98E-07 1.17E-06 -1.057 5.93E-07 -1.14546 insulin-like 4 (placenta) INSL4 NM_002195 2.07E-08 2.28E-08 -1.05698 1.63E-08 -1.09834 BRCA2 and CDKN1A interacting protein BCCIP NM_016567 /// NM_0784685.12E-08 /// NM_0784691.36E-07 -1.05519 2.32E-08 -1.29166 chromosome 18 open reading frame 21 C18orf21 NM_031446 2.17E-08 2.77E-08 -1.05497 1.52E-08 -1.12968 Splicing factor, /serine-rich, 46kD SRP46 NM_032102 1.20E-07 2.44E-07 -1.05478 6.17E-08 -1.23603 basic transcription factor 3-like 4 BTF3L4 NM_152265 2.02E-09 3.84E-09 -1.05027 1.10E-09 -1.20937 calcium activated nucleotidase 1 CANT1 NM_138793 7.45E-08 7.26E-08 -1.04535 6.49E-08 -1.05888 chromosome 14 open reading frame 111 C14orf111 NM_015962 0.00104121 0.00081564 -1.0398 0.000811973 -1.04049 NIMA (never in mitosis gene a)-related kinase 3 NEK3 NM_002498 /// NM_1527204.40E-06 6.34E-06 -1.03778 2.64E-06 -1.15327 mitogen-activated protein kinase kinase kinase 9 MAP3K9 NM_033141 8.97E-09 5.10E-08 -1.03526 3.15E-09 -1.42023 hypothetical protein HH114 /// hypothetical protein HH114 HH114 NM_032499 1.54E-08 2.29E-08 -1.03356 9.67E-09 -1.13986 CDNA FLJ43100 fis, clone CTONG2003100 ------1.59E-05 2.72E-05 -1.03346 8.34E-06 -1.19641 nuclear receptor subfamily 2, group F, member 6 NR2F6 NM_005234 2.23E-07 1.93E-07 -1.03187 2.16E-07 -1.01891 ------3.24E-08 2.64E-08 -1.03152 3.42E-08 -1.00145 phosphatidylcholine transfer protein PCTP NM_021213 1.93E-09 1.05E-08 -1.02979 6.89E-10 -1.40044 aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase AASDHPPT NM_015423 7.35E-08 9.23E-08 -1.02861 5.16E-08 -1.09961 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfSMARCC1 NM_003074 2.67E-09 3.32E-09 -1.0283 1.93E-09 -1.09283 zinc finger, DHHC-type containing 3 ZDHHC3 NM_016598 2.18E-10 3.13E-10 -1.02697 1.43E-10 -1.12131 KIAA1702 protein KIAA1702 --- 0.000741318 0.000941365 -1.02098 0.000401202 -1.15521 thioesterase superfamily member 4 THEM4 NM_053055 /// NM_1768533.59E-08 5.74E-08 -1.02016 2.14E-08 -1.1415 F-box protein 8 FBXO8 NM_012180 7.58E-06 5.13E-06 -1.01989 9.05E-06 -0.951908 fibroblast growth factor receptor 4 FGFR4 NM_002011 /// NM_0229632.23E-06 /// NM_2136471.94E-06 -1.01963 2.06E-06 -1.01235 acidic (leucine-rich) nuclear phosphoprotein 32 family, member A ANP32A NM_006305 0.000695738 0.000696268 -1.01837 0.000448296 -1.08496 hypothetical protein MGC11102 MGC11102 NM_032325 4.34E-10 7.07E-10 -1.01782 2.61E-10 -1.13836 AT rich interactive domain 1A (SWI- like) ARID1A NM_006015 /// NM_0184504.51E-10 /// NM_1391354.49E-10 -1.01765 3.98E-10 -1.03137 diaphanous homolog 2 (Drosophila) DIAPH2 NM_006729 /// NM_0073091.88E-07 1.40E-07 -1.01564 2.18E-07 -0.96495 Transcribed locus, moderately similar to XP_524454.1 PREDICTED: hypothetical--- pro --- 8.39E-05 0.000289447 -1.01478 3.12E-05 -1.3612 PHD finger protein 19 PHF19 NM_001009936 /// 2.22E-07NM_015651 3.47E-07 -1.0134 1.33E-07 -1.13258 stress-associated endoplasmic reticulum protein 1 SERP1 NM_014445 2.77E-09 3.15E-09 -1.01018 2.15E-09 -1.05486 family with sequence similarity 96, member A FAM96A NM_001014812 /// 4.56E-12NM_032231 4.07E-12 -1.00941 4.54E-12 -0.997208 E74-like factor 4 (ets domain transcription factor) ELF4 NM_001421 1.00E-07 1.53E-07 -1.00712 6.08E-08 -1.11959 hypothetical protein FLJ11155 FLJ11155 NM_018342 3.84E-07 3.74E-07 -1.00661 3.29E-07 -1.02185 Lysophosphatidylglycerol acyltransferase 1 LPGAT1 NM_014873 5.12E-08 6.13E-08 -1.00581 3.74E-08 -1.06424 malate dehydrogenase 2, NAD (mitochondrial) MDH2 NM_005918 4.28E-11 3.22E-11 -1.0047 5.16E-11 -0.953124 ring finger protein 13 RNF13 NM_007282 /// NM_1833811.79E-09 /// NM_1833821.88E-09 /// NM_183383-0.998348 /// NM_1833841.49E-09 -1.0249 pleiomorphic adenoma gene-like 1 PLAGL1 NM_002656 /// NM_0067182.77E-05 7.10E-05 -0.99701 1.18E-05 -1.25066 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfSMARCC1 NM_003074 6.25E-08 9.98E-08 -0.993836 3.71E-08 -1.11305 protein phosphatase 4 (formerly X), catalytic subunit PPP4C NM_002720 3.70E-08 6.15E-08 -0.993433 2.15E-08 -1.12009 Polymerase (RNA) II (DNA directed) polypeptide D POLR2D NM_004805 3.10E-07 3.11E-07 -0.992235 2.59E-07 -1.01339 sorting nexin 5 SNX5 NM_014426 /// NM_1522277.74E-11 7.01E-11 -0.991648 7.54E-11 -0.983645 mediator of RNA polymerase II transcription, subunit 8 homolog (yeast) MED8 NM_001001651 /// 7.92E-08NM_001001653 1.11E-07/// NM_001001654-0.989578 /// NM_0528775.11E-08 /// NM_201542-1.08239 family with sequence similarity 54, member A FAM54A NM_138419 2.50E-07 3.13E-07 -0.982468 1.74E-07 -1.05186 apoptosis, caspase activation inhibitor AVEN NM_020371 3.07E-07 7.46E-07 -0.981955 1.43E-07 -1.18996 polymerase (RNA) II (DNA directed) polypeptide D POLR2D NM_004805 3.43E-09 8.53E-09 -0.980353 1.63E-09 -1.1823 glycine amidinotransferase (L-arginine:glycine amidinotransferase) GATM NM_001482 0.000668536 0.000680578 -0.980307 0.000426025 -1.04848 hypothetical protein MGC18216 MGC18216 --- 1.53E-05 0.000178732 -0.980028 4.47E-06 -1.5636 Peroxisome proliferative activated receptor, gamma PPARG NM_005037 /// NM_0158694.51E-08 /// NM_1387118.27E-08 /// NM_138712-0.979451 2.47E-08 -1.12446 Supplemental Table 2: Genes that show altered expression in A549 cells in the presence of exogenously added let-7

v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian) MAFK NM_002360 2.70E-09 1.00E-08 -0.97836 1.09E-09 -1.2569 GATA zinc finger domain containing 2A p66alpha NM_017660 4.03E-06 6.84E-06 -0.978005 2.18E-06 -1.12203 coagulation factor V (proaccelerin, labile factor) F5 NM_000130 2.38E-07 3.70E-07 -0.974922 1.42E-07 -1.0891 solute carrier family 39 (zinc transporter), member 14 SLC39A14 NM_015359 0.00328188 0.00291112 -0.974225 0.0020871 -1.02814 chromosome 1 open reading frame 121 C1orf121 NM_016076 2.16E-08 2.42E-08 -0.973566 1.68E-08 -1.01499 Helicase, lymphoid-specific HELLS NM_018063 0.000331233 0.000431415 -0.972471 0.000183892 -1.09417 CDNA FLJ41999 fis, clone SPLEN2029683 ------2.45E-08 2.96E-08 -0.965035 1.79E-08 -1.02216 H1 histone family, member X H1FX NM_006026 6.36E-07 8.89E-07 -0.963653 4.03E-07 -1.05721 hypothetical protein LOC283464 LOC283464 XM_290597 5.15E-07 6.44E-05 -0.958513 1.36E-07 -2.0151 UTP15, U3 small nucleolar ribonucleoprotein, homolog (yeast) UTP15 NM_032175 9.14E-07 1.09E-06 -0.957456 6.45E-07 -1.01897 tetratricopeptide repeat domain 9C TTC9C NM_173810 8.06E-09 9.52E-09 -0.95491 6.03E-09 -1.00557 p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) PAK1 NM_002576 2.84E-06 3.29E-06 -0.95236 2.01E-06 -1.00991 estrogen receptor associated, antigen, 9 EBAG9 NM_004215 /// NM_1981201.05E-07 1.29E-07 -0.952269 7.45E-08 -1.01384 nephroblastoma overexpressed gene NOV NM_002514 3.02E-05 4.03E-05 0.951701 1.81E-05 1.05224 ------3.54E-09 1.12E-07 0.952156 9.59E-10 1.63505 dedicator of cytokinesis 11 DOCK11 NM_144658 0.000229176 0.000380939 0.9536 0.000111912 1.12668 pellino homolog 1 (Drosophila) PELI1 NM_020651 9.34E-06 1.50E-05 0.954668 5.15E-06 1.08778 cornifelin /// cornifelin CNFN NM_032488 6.68E-07 3.16E-06 0.954947 2.41E-07 1.29248 phosphatidylinositol transfer protein, cytoplasmic 1 PITPNC1 NM_012417 /// NM_1816717.98E-07 7.09E-07 0.956114 7.36E-07 0.951947 ferredoxin reductase FDXR NM_004110 /// NM_0244171.33E-08 3.27E-08 0.95672 6.30E-09 1.15351 dihydropyrimidine dehydrogenase DPYD NM_000110 0.00021167 0.000192165 0.961011 0.000158843 0.986028 SH3 domain binding glutamic acid-rich protein like 3 /// SH3 domain binding glutSH3BGRL3 NM_031286 1.01E-09 9.79E-10 0.961367 9.14E-10 0.968893 G protein-coupled receptor 161 GPR161 NM_007369 /// NM_1538329.68E-05 0.000111754 0.961936 6.18E-05 1.03942 Clone 24841 mRNA sequence ------5.57E-06 7.45E-06 0.963197 3.49E-06 1.05569 secretory carrier membrane protein 1 SCAMP1 NM_004866 /// NM_0528222.09E-05 0.000229661 0.96366 6.16E-06 1.5303 fibronectin 1 FN1 NM_002026 /// NM_0540345.11E-08 /// NM_2124745.09E-08 /// NM_2124750.965085 /// NM_2124764.38E-08 /// NM_2124780.981728 microtubule associated monoxygenase, calponin and LIM domain containing 2 MICAL2 NM_014632 6.54E-11 1.67E-10 0.965698 3.09E-11 1.1668 La ribonucleoprotein domain family, member 6 LARP6 NM_018357 /// NM_1979581.35E-06 2.51E-06 0.966255 7.06E-07 1.12276 Hypothetical protein FLJ36031 FLJ36031 NM_175884 5.63E-06 6.33E-06 0.96985 4.03E-06 1.02424 melanoma cell adhesion molecule MCAM NM_006500 4.25E-06 1.87E-05 0.970539 1.54E-06 1.31373 adenylate cyclase 1 (brain) ADCY1 NM_021116 4.76E-05 0.000218243 0.970823 1.65E-05 1.35695 hypothetical protein DKFZp434J1015 DKFZp434J1015XM_496849 /// XM_4992579.05E-05 0.000221626 0.972149 3.80E-05 1.22581 Similar to echinoderm microtubule associated protein like 5 --- XM_376056 4.91E-07 4.51E-07 0.972329 4.42E-07 0.974766 EPH receptor B2 EPHB2 NM_004442 /// NM_0174495.56E-06 6.83E-06 0.972619 3.72E-06 1.0468 FLJ43339 protein FLJ43339 NM_207380 4.40E-07 4.49E-06 0.973493 1.34E-07 1.47095 L1 cell adhesion molecule L1CAM NM_000425 /// NM_0240032.09E-08 2.84E-08 0.975777 1.40E-08 1.05769 septin 6 /// cytokine-like nuclear factor n-pac SEPT6 /// N-PACNM_015129 /// NM_0325697.67E-08 /// NM_1457996.94E-08 /// NM_1458000.97688 /// NM_1458027.19E-08 0.972828 purine-rich element binding protein B PURB NM_033224 7.22E-08 1.21E-07 0.978667 4.16E-08 1.10619 complement component 1, r subcomponent C1R NM_001733 1.37E-05 2.52E-05 0.979279 6.89E-06 1.14933 tumor necrosis factor receptor superfamily, member 25 TNFRSF25 NM_003790 /// NM_1489653.65E-05 /// NM_1489666.66E-05 /// NM_1489670.979773 /// NM_1489681.80E-05 /// NM_1489691.1565 melanoma cell adhesion molecule MCAM NM_006500 1.08E-07 2.03E-07 0.980413 5.75E-08 1.134 sulfide quinone reductase-like (yeast) SQRDL NM_021199 1.68E-09 1.61E-09 0.980606 1.53E-09 0.985596 guanine nucleotide binding protein (G protein), beta polypeptide 1 GNB1 NM_002074 8.59E-10 7.81E-09 0.981041 2.72E-10 1.43187 N-myc downstream regulated gene 1 NDRG1 NM_006096 3.33E-06 4.40E-06 0.985049 2.13E-06 1.07457 solute carrier family 44, member 1 SLC44A1 NM_022109 /// NM_0805462.19E-07 5.30E-07 0.986132 1.02E-07 1.19294 metallothionein 1G MT1G NM_005950 5.61E-08 7.99E-08 0.987492 3.60E-08 1.08181 four and a half LIM domains 2 FHL2 NM_001450 /// NM_2015551.32E-10 /// NM_2015561.31E-10 /// NM_2015570.989482 1.18E-10 1.00156 claudin 23 CLDN23 NM_194284 3.82E-05 3.19E-05 0.990274 3.37E-05 0.98372 CD9 antigen (p24) CD9 NM_001769 2.46E-09 9.52E-09 0.990434 9.75E-10 1.28095 hypothetical protein FLJ22313 FLJ22313 NM_022373 2.58E-08 9.97E-08 0.991545 1.02E-08 1.28679 RAB3B, member RAS oncogene family RAB3B NM_002867 2.24E-07 1.77E-07 0.99338 2.40E-07 0.959289 adenylate kinase 5 AK5 NM_012093 /// NM_1748587.64E-05 0.000115247 0.993779 4.11E-05 1.13615 CDNA FLJ42405 fis, clone ASTRO3000474 ------3.17E-05 4.42E-05 0.995824 1.85E-05 1.11167 ------1.01E-06 6.78E-06 0.996184 3.34E-07 1.42367 lectin, galactoside-binding, soluble, 3 (galectin 3) /// galectin-3 internal gen LGALS3 /// GALIGNM_002306 /// NM_1943273.89E-10 2.71E-09 0.99742 1.31E-10 1.4025 nexilin (F actin binding protein) NEXN NM_144573 0.000362144 0.000257278 0.998082 0.000337997 0.961052 pleckstrin homology-like domain, family B, member 3 PHLDB3 NM_198850 1.18E-08 1.01E-08 0.998405 1.19E-08 0.97997 cytochrome P450, family 3, subfamily A, polypeptide 5 CYP3A5 NM_000777 6.49E-06 7.51E-06 0.999779 4.52E-06 1.06319 Supplemental Table 2: Genes that show altered expression in A549 cells in the presence of exogenously added let-7

cytochrome P450, family 3, subfamily A, polypeptide 5 CYP3A5 NM_000777 1.71E-05 3.62E-05 1.00405 8.00E-06 1.21149 tight junction protein 2 (zona occludens 2) TJP2 NM_004817 /// NM_2016294.73E-07 3.69E-07 1.00457 5.09E-07 0.967634 twist homolog 1 (acrocephalosyndactyly 3; Saethre-Chotzen syndrome) (Drosophila)TWIST1 NM_000474 0.000323288 0.000350331 1.00928 0.000204598 1.08696 synovial sarcoma translocation gene on chromosome 18-like 1 SS18L1 NM_015558 /// NM_1989352.97E-08 8.09E-08 1.00936 1.33E-08 1.23986 TBC1 domain family, member 2 TBC1D2 NM_018421 2.52E-06 8.76E-06 1.01132 9.94E-07 1.31265 talin 1 TLN1 NM_006289 0.000610857 0.000991175 1.01546 0.000289461 1.21185 stimulated by retinoic acid gene 6 homolog (mouse) /// stimulated by retinoic STRA6ac NM_022369 3.48E-07 5.69E-07 1.01623 2.00E-07 1.14761 SEC24 related gene family, member A (S. cerevisiae) SEC24A XM_094581 6.22E-07 7.71E-07 1.01984 4.30E-07 1.092 ribonucleotide reductase M2 B (TP53 inducible) RRM2B NM_015713 4.69E-08 1.60E-07 1.0216 1.92E-08 1.30234 THUMP domain containing 1 THUMPD1 NM_017736 8.23E-06 1.38E-05 1.02205 4.42E-06 1.17462 acid phosphatase-like 2 ACPL2 NM_152282 1.00E-07 1.49E-06 1.02217 2.91E-08 1.61277 EPH receptor B2 EPHB2 NM_004442 /// NM_0174497.50E-06 9.32E-06 1.02331 4.93E-06 1.10579 synaptogyrin 3 SYNGR3 NM_004209 1.70E-06 1.02E-05 1.02426 5.74E-07 1.44479 nuclear receptor binding SET domain protein 1 NSD1 NM_022455 /// NM_1723498.16E-07 7.77E-07 1.0255 7.03E-07 1.03771 KIAA1571 protein KIAA1571 XM_371590 5.02E-07 8.65E-07 1.02566 2.79E-07 1.17076 STEAP family member 3 STEAP3 NM_001008410 /// 1.12E-08NM_018234 /// NM_1829151.55E-07 1.02825 3.31E-09 1.59385 Niemann-Pick disease, type C2 NPC2 NM_006432 3.80E-10 3.28E-10 1.02901 3.88E-10 1.00988 nucleosome assembly protein 1-like 3 NAP1L3 NM_004538 3.51E-05 2.92E-05 1.02976 3.13E-05 1.02088 Transcribed locus ------8.90E-09 3.14E-08 1.03275 3.63E-09 1.31934 leucine-rich repeat-containing G protein-coupled receptor 6 LGR6 NM_001017403 /// 3.67E-08NM_001017404 1.17E-07/// NM_021636 1.03416 1.55E-08 1.30305 solute carrier family 25, member 37 SLC25A37 NM_016612 /// NM_0185791.07E-07 9.21E-08 1.03423 1.06E-07 1.01744 chondroitin sulfate proteoglycan 5 (neuroglycan C) CSPG5 NM_006574 8.15E-05 5.30E-05 1.03726 9.27E-05 0.964625 THUMP domain containing 1 THUMPD1 NM_017736 3.71E-06 6.98E-06 1.03892 1.90E-06 1.21474 RAP2B, member of RAS oncogene family RAP2B NM_002886 5.44E-10 1.26E-09 1.04086 2.68E-10 1.23834 solute carrier family 25, member 37 SLC25A37 NM_016612 /// NM_0185799.26E-05 8.53E-05 1.04184 7.14E-05 1.06638 collagen, type IV, alpha 5 (Alport syndrome) COL4A5 NM_000495 /// NM_0333803.62E-09 /// NM_0333812.97E-09 1.04232 3.85E-09 1.01245 PYD and CARD domain containing PYCARD NM_013258 /// NM_1451821.23E-09 /// NM_1451832.86E-09 1.04253 6.07E-10 1.2414 potassium channel, subfamily K, member 1 KCNK1 NM_002245 2.25E-06 1.86E-06 1.04429 2.19E-06 1.0245 tripartite motif-containing 8 /// tripartite motif-containing 8 TRIM8 NM_030912 4.32E-08 1.89E-07 1.04604 1.63E-08 1.38537 brain-specific angiogenesis inhibitor 2 BAI2 NM_001703 3.01E-06 2.78E-06 1.04633 2.60E-06 1.05432 lecithin-cholesterol acyltransferase LCAT NM_000229 4.45E-07 5.92E-07 1.04715 2.93E-07 1.13659 cadherin 19, type 2 CDH19 NM_021153 1.49E-05 9.82E-06 1.0482 1.79E-05 0.973162 CDNA FLJ35260 fis, clone PROST2004258 ------2.79E-07 1.78E-07 1.04922 3.92E-07 0.957292 5'-nucleotidase, ecto (CD73) NT5E NM_002526 1.37E-07 2.30E-07 1.04965 7.79E-08 1.18943 Chromosome 8 open reading frame 1 C8orf1 NM_004337 1.11E-07 4.30E-06 1.05073 2.95E-08 1.87893 hypothetical protein FLJ22313 FLJ22313 NM_022373 5.71E-10 3.31E-09 1.05343 2.01E-10 1.44359 aquaporin 3 AQP3 NM_004925 3.93E-08 3.98E-08 1.05568 3.32E-08 1.07771 KDEL (Lys-Asp-Glu-Leu) containing 1 KDELC1 NM_024089 9.57E-08 4.40E-07 1.05697 3.54E-08 1.41329 sushi domain containing 2 SUSD2 NM_019601 1.05E-07 3.30E-07 1.05811 4.44E-08 1.33329 MRNA; cDNA DKFZp586B211 (from clone DKFZp586B211) ------0.000290699 0.000191518 1.05986 0.000301382 0.995991 Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain,CITED2 NM_006079 6.87E-06 1.11E-05 1.06596 3.79E-06 1.21502 plasminogen activator, tissue PLAT NM_000930 /// NM_0009313.57E-05 /// NM_0330113.37E-05 1.06683 2.81E-05 1.09144 topoisomerase (DNA) I, mitochondrial TOP1MT NM_052963 2.85E-07 3.45E-06 1.06856 8.49E-08 1.64876 four and a half LIM domains 1 FHL1 NM_001449 3.91E-08 5.63E-08 1.07478 2.50E-08 1.17938 diacylglycerol kinase, alpha 80kDa DGKA NM_001345 /// NM_2014444.67E-08 /// NM_2014454.73E-08 /// NM_2015541.07487 3.94E-08 1.09775 latent transforming growth factor beta binding protein 3 LTBP3 NM_021070 1.63E-05 5.15E-05 1.07889 6.44E-06 1.39796 neuronal guanine nucleotide exchange factor NGEF NM_019850 5.90E-07 9.57E-07 1.08105 3.39E-07 1.22059 hypothetical protein LOC284611 LOC284611 NM_001010883 1.77E-07 1.28E-07 1.08174 2.11E-07 1.02146 olfactomedin 1 OLFM1 NM_006334 /// NM_0142791.07E-05 /// NM_0581992.60E-05 1.08439 4.72E-06 1.33765 potassium intermediate/small conductance calcium-activated channel, subfamilyKCNN4 N, NM_002250 3.80E-06 2.98E-06 1.08558 3.87E-06 1.05221 guanine nucleotide binding protein (G protein), beta polypeptide 1 GNB1 NM_002074 3.33E-09 3.55E-08 1.0858 1.02E-09 1.62258 Follicular lymphoma variant translocation 1 FVT1 NM_002035 2.38E-07 2.73E-07 1.08812 1.78E-07 1.14327 chromosome 1 open reading frame 139 C1orf139 NM_001002292 /// 1.67E-06NM_024911 9.28E-07 1.09039 2.79E-06 0.956854 , serine, 1 (trypsin 1) /// protease, serine, 2 (trypsin 2) /// protease PRSS1 /// PRSS2NM_002769 /// PRSS3 /// TRNM_002770Y62.88E-05 /// NM_0027711.85E-05 /// NR_0012961.0904 3.49E-05 1.00686 four and a half LIM domains 1 FHL1 NM_001449 5.48E-06 3.69E-06 1.096 6.66E-06 1.02067 U2AF homology motif (UHM) kinase 1 UHMK1 NM_175866 1.61E-05 1.56E-05 1.09608 1.27E-05 1.12366 obscurin-like 1 OBSL1 XM_051017 7.53E-06 2.04E-05 1.10155 3.21E-06 1.38075 homeodomain interacting protein kinase 3 HIPK3 NM_005734 2.91E-05 4.61E-05 1.10261 1.57E-05 1.2627 Supplemental Table 2: Genes that show altered expression in A549 cells in the presence of exogenously added let-7

Solute carrier family 25, member 37 MSCP NM_016612 /// NM_0185794.69E-08 5.07E-08 1.10508 3.73E-08 1.14441 solute carrier family 25, member 37 SLC25A37 NM_016612 /// NM_0185798.94E-09 8.49E-09 1.10852 8.14E-09 1.11376 Ets homologous factor EHF NM_012153 2.82E-05 0.000180183 1.10929 9.08E-06 1.62547 aldehyde oxidase 1 AOX1 NM_001159 5.49E-07 4.31E-07 1.11296 5.82E-07 1.07464 capping protein (actin filament), gelsolin-like CAPG NM_001747 8.67E-09 2.30E-08 1.11687 3.98E-09 1.36258 CDNA FLJ34585 fis, clone KIDNE2008758 ------6.10E-06 3.99E-06 1.11706 7.67E-06 1.03207 dual specificity phosphatase 6 DUSP6 NM_001946 /// NM_0226523.56E-06 2.04E-06 1.11959 5.55E-06 0.992866 ring finger protein 144 RNF144 NM_014746 3.81E-09 1.21E-08 1.12632 1.62E-09 1.41296 secretory carrier membrane protein 1 SCAMP1 NM_004866 /// NM_0528226.75E-07 4.41E-06 1.12659 2.24E-07 1.60064 fibroblast growth factor binding protein 1 FGFBP1 NM_005130 5.29E-06 4.94E-06 1.12911 4.46E-06 1.14324 DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked DDX3Y NM_004660 8.58E-07 5.43E-07 1.13006 1.19E-06 1.03038 epithelial membrane protein 2 EMP2 NM_001424 1.28E-09 1.56E-08 1.13007 3.85E-10 1.71634 ribosomal protein S6 kinase, 90kDa, polypeptide 5 RPS6KA5 NM_004755 /// NM_1823987.24E-09 7.01E-09 1.13099 6.48E-09 1.14106 EGF-like-domain, multiple 4 EGFL4 NM_001410 6.38E-09 7.13E-09 1.13164 5.01E-09 1.17778 SPOC domain containing 1 SPOCD1 NM_144569 3.66E-06 2.49E-06 1.13384 4.43E-06 1.05822 lysosomal-associated membrane protein 2 LAMP2 NM_002294 /// NM_0139951.59E-07 7.25E-07 1.1349 5.85E-08 1.51894 nudix (nucleoside diphosphate linked moiety X)-type motif 15 NUDT15 NM_018283 1.12E-10 6.41E-09 1.13598 2.95E-11 2.07886 fibrillin 1 (Marfan syndrome) FBN1 NM_000138 8.56E-08 2.50E-07 1.13974 3.72E-08 1.41887 metallothionein 1X MT1X NM_005952 1.55E-08 8.18E-08 1.14039 5.56E-09 1.54913 glutamate-ammonia ligase (glutamine synthetase) GLUL NM_001033044 /// 3.22E-07NM_001033056 2.26E-07/// NM_002065 1.14047 3.97E-07 1.06849 brain expressed X-linked-like 1 BEXL1 XM_043653 8.98E-05 8.14E-05 1.15025 7.05E-05 1.17201 activated leukocyte cell adhesion molecule ALCAM NM_001627 2.89E-09 6.08E-08 1.15253 8.10E-10 1.88013 laminin, beta 3 LAMB3 NM_000228 /// NM_0010174024.57E-06 3.07E-06 1.15371 5.61E-06 1.07281 hypothetical protein LOC51315 LOC51315 NM_016618 3.87E-07 2.74E-06 1.15402 1.27E-07 1.65406 transmembrane protein 16A TMEM16A NM_018043 5.89E-06 5.38E-06 1.15567 5.06E-06 1.16421 tetratricopeptide repeat domain 7B TTC7B NM_001010854 4.17E-07 3.63E-07 1.16044 3.97E-07 1.14828 ankyrin repeat domain 22 ANKRD22 NM_144590 0.000555395 0.000401101 1.16152 0.000494063 1.12768 runt-related transcription factor 2 RUNX2 NM_001015051 /// 1.15E-06NM_001024630 8.11E-07/// NM_004348 1.16217 1.38E-06 1.09176 polymerase I and transcript release factor PTRF NM_012232 3.41E-06 2.13E-06 1.16331 4.67E-06 1.05855 aldehyde dehydrogenase 1 family, member A3 ALDH1A3 NM_000693 3.44E-05 1.62E-05 1.16836 6.92E-05 0.971771 myosin ID MYO1D NM_015194 5.71E-06 5.31E-06 1.16887 4.82E-06 1.18266 transcription elongation factor A (SII)-like 3 TCEAL3 NM_001006933 /// 1.66E-07NM_032926 2.49E-07 1.16982 1.02E-07 1.29767 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) CD47 NM_001025079 /// 5.34E-07NM_001025080 1.26E-06/// NM_001777 ///1.17016 NM_198793 2.51E-07 1.41366 Hypothetical protein FLJ14681 FLJ14681 NM_032824 1.36E-07 1.15E-07 1.17045 1.36E-07 1.1475 renal tumor antigen RAGE NM_014226 9.90E-10 2.36E-09 1.17292 4.80E-10 1.40288 hypothetical protein FLJ22313 FLJ22313 NM_022373 1.93E-07 3.61E-07 1.17514 1.03E-07 1.35852 metallothionein 1E (functional) MT1E NM_175617 7.74E-05 8.78E-05 1.17641 5.05E-05 1.26393 trinucleotide repeat containing 6A TNRC6A NM_014494 /// NM_0208476.19E-09 3.36E-09 1.17937 1.18E-08 1.02302 glutamate-ammonia ligase (glutamine synthetase) GLUL NM_001033044 /// 2.29E-07NM_001033056 1.12E-07/// NM_002065 1.1811 5.02E-07 0.992733 Ras-like without CAAX 1 RIT1 NM_006912 1.05E-05 5.52E-06 1.18193 1.80E-05 1.02268 epithelial membrane protein 1 EMP1 NM_001423 9.68E-08 7.89E-08 1.18279 1.01E-07 1.14949 tryptophan 2,3-dioxygenase TDO2 NM_005651 4.47E-06 3.61E-06 1.18366 4.40E-06 1.15571 stearoyl-CoA desaturase 5 SCD5 NM_024906 0.00033124 0.000432394 1.18555 0.000183627 1.3346 colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophagCSF2RA NM_006140 /// NM_1722452.30E-08 /// NM_1722461.81E-08 /// NM_1722471.1924 /// NM_1722482.55E-08 /// NM_1722491.14617 CD44 antigen (homing function and Indian blood group system) CD44 NM_000610 /// NM_0010013895.97E-07 /// NM_0010013902.97E-07 ///1.19315 NM_0010013911.25E-06 /// NM_0010013921.00824 formin-like 2 FMNL2 NM_001004417 /// 3.17E-10NM_001004421 2.19E-10/// NM_0010044221.1957 /// NM_0529054.25E-10 1.11 tumor necrosis factor receptor superfamily, member 11a, NFKB activator TNFRSF11A NM_003839 2.60E-08 1.14E-08 1.19952 7.59E-08 0.965895 metallothionein 1H MT1H NM_005951 3.98E-09 1.13E-08 1.20145 1.78E-09 1.47993 HtrA serine peptidase 1 HTRA1 NM_002775 3.98E-07 3.58E-07 1.20223 3.69E-07 1.198 hypothetical protein FLJ39370 FLJ39370 NM_152400 1.45E-08 4.70E-07 1.2034 3.92E-09 2.08238 chromosome 2 open reading frame 32 C2orf32 NM_015463 3.43E-07 6.89E-07 1.20348 1.76E-07 1.411 chemokine (C-C motif) ligand 26 CCL26 NM_006072 5.34E-08 1.09E-07 1.20508 2.75E-08 1.41147 insulin-like growth factor binding protein 6 IGFBP6 NM_002178 1.51E-10 4.89E-10 1.20828 6.41E-11 1.5171 solute carrier family 4, sodium bicarbonate transporter-like, member 11 SLC4A11 NM_032034 7.40E-06 1.62E-05 1.21002 3.47E-06 1.46001 Voltage gated channel like 1 VGCNL1 NM_052867 2.04E-07 1.42E-07 1.21241 2.55E-07 1.13298 chondroitin sulfate GalNAcT-2 GALNACT-2 NM_018590 6.18E-08 1.07E-07 1.21369 3.50E-08 1.3793 family with sequence similarity 61, member A FAM61A NM_015578 5.99E-09 1.68E-07 1.21578 1.64E-09 2.05954 scavenger receptor class A, member 3 SCARA3 NM_016240 /// NM_1828261.92E-07 7.22E-07 1.21673 7.54E-08 1.58096 Supplemental Table 2: Genes that show altered expression in A549 cells in the presence of exogenously added let-7

microtubule associated monoxygenase, calponin and LIM domain containing 2 MICAL2 NM_014632 4.69E-10 6.22E-10 1.21905 3.24E-10 1.31162 integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) ITGA2 NM_002203 4.50E-07 2.55E-07 1.22125 7.51E-07 1.07658 guanine deaminase GDA NM_004293 3.97E-08 6.36E-08 1.22196 2.37E-08 1.368 hypothetical protein FLJ36748 FLJ36748 NM_152406 1.10E-07 1.09E-07 1.22502 9.39E-08 1.2456 synaptotagmin XIII SYT13 NM_020826 6.95E-08 4.69E-08 1.22783 9.20E-08 1.13657 cytidine deaminase CDA NM_001785 2.03E-08 3.39E-08 1.23056 1.18E-08 1.38757 ATPase, Class II, type 9A ATP9A NM_006045 2.12E-09 3.90E-09 1.23216 1.18E-09 1.40973 stanniocalcin 1 STC1 NM_003155 2.45E-05 1.03E-05 1.23244 6.43E-05 0.979044 latent transforming growth factor beta binding protein 4 LTBP4 NM_003573 1.13E-05 1.07E-05 1.23297 9.16E-06 1.25665 MRNA; cDNA DKFZp586B211 (from clone DKFZp586B211) ------1.78E-07 1.27E-07 1.23313 2.14E-07 1.16131 transgelin TAGLN NM_001001522 /// 1.55E-07NM_003186 1.77E-06 1.23992 4.66E-08 1.89174 polymerase I and transcript release factor PTRF NM_012232 8.60E-06 4.75E-06 1.24059 1.38E-05 1.08904 secretory carrier membrane protein 1 SCAMP1 NM_004866 /// NM_0528221.14E-07 1.70E-06 1.24372 3.31E-08 1.96438 hypothetical LOC554202 LOC554202 --- 1.63E-07 1.44E-07 1.24378 1.54E-07 1.23429 obscurin-like 1 OBSL1 XM_051017 1.15E-05 1.72E-05 1.24627 6.55E-06 1.40281 interferon, gamma-inducible protein 16 IFI16 NM_005531 4.28E-07 3.07E-07 1.24753 5.09E-07 1.17592 nexilin (F actin binding protein) NEXN NM_144573 3.05E-07 1.83E-07 1.25023 4.68E-07 1.12078 colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophagCSF2RA NM_006140 /// NM_1722453.13E-08 /// NM_1722466.23E-08 /// NM_1722471.25215 /// NM_1722481.64E-08 /// NM_1722491.45838 spermidine/spermine N1-acetyltransferase SAT NM_002970 4.50E-09 4.25E-09 1.2547 4.13E-09 1.25867 Similar to 60S ribosomal protein L35 RPL35 NM_007209 1.75E-09 3.49E-09 1.25494 9.33E-10 1.45594 STEAP family member 3 STEAP3 NM_001008410 /// 1.75E-06NM_018234 /// NM_1829155.32E-06 1.25594 7.28E-07 1.5916 collagen, type V, alpha 1 COL5A1 NM_000093 8.40E-08 6.90E-08 1.25683 8.72E-08 1.22346 pleckstrin homology-like domain, family A, member 1 PHLDA1 NM_007350 2.88E-08 1.53E-08 1.26636 5.57E-08 1.09284 epithelial membrane protein 2 EMP2 NM_001424 4.18E-11 6.40E-10 1.27709 1.22E-11 1.98939 transforming growth factor, alpha TGFA NM_003236 1.73E-11 4.18E-11 1.27734 8.41E-12 1.52774 pleckstrin homology domain containing, family H (with MyTH4 domain) memberPLEKHH2 2 NM_172069 9.44E-06 4.91E-06 1.2785 1.67E-05 1.10069 Transcribed locus, weakly similar to XP_517655.1 PREDICTED: similar to KIAA0825------6.07E-08 2.63E-07 1.2794 2.29E-08 1.69336 colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophagCSF2RA NM_006140 /// NM_1722456.34E-09 /// NM_1722461.18E-08 /// NM_1722471.28425 /// NM_1722483.49E-09 /// NM_1722491.47423 dual specificity phosphatase 6 DUSP6 NM_001946 /// NM_0226524.50E-06 6.16E-06 1.28496 2.80E-06 1.41322 Transcribed locus ------7.55E-06 3.97E-06 1.28673 1.32E-05 1.11134 Ras association (RalGDS/AF-6) domain family 2 RASSF2 NM_014737 /// NM_1707731.84E-07 /// NM_1707742.29E-07 1.29246 1.29E-07 1.38077 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferGALNT12ase NM_024642 1.03E-08 1.02E-08 1.29611 9.06E-09 1.31353 C1q domain containing 1 C1QDC1 NM_001002259 /// 8.38E-12NM_023925 /// NM_0321561.62E-11 1.29697 4.58E-12 1.49384 proline-rich protein PRP2 PRP2 NM_173490 1.52E-07 1.18E-07 1.29748 1.66E-07 1.24725 KDEL (Lys-Asp-Glu-Leu) containing 2 KDELC2 NM_153705 5.94E-08 1.45E-07 1.30211 2.80E-08 1.5719 solute carrier family 22 (organic cation transporter), member 4 SLC22A4 NM_003059 2.31E-08 1.85E-08 1.30467 2.50E-08 1.26035 transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)TFAP2C NM_003222 9.19E-08 1.65E-07 1.30471 5.06E-08 1.49483 collagen, type XII, alpha 1 COL12A1 NM_004370 /// NM_0806450.000355421 0.000195224 1.30868 0.000468136 1.15907 integrin, alpha 6 ITGA6 NM_000210 5.71E-09 2.32E-09 1.30931 2.05E-08 1.02289 rabaptin, RAB GTPase binding effector protein 2 RABEP2 NM_024816 5.65E-08 3.53E-08 1.31398 8.35E-08 1.19084 zinc finger protein 680 ZNF680 NM_178558 1.27E-06 5.86E-07 1.3147 3.02E-06 1.08261 ATPase, Class VI, type 11C ATP11C NM_001010986 /// 4.28E-10NM_173694 7.35E-10 1.31495 2.49E-10 1.48453 solute carrier family 11 (proton-coupled divalent metal ion transporters), membeSLC11A2 NM_000617 1.24E-07 5.41E-08 1.31564 3.55E-07 1.05908 RasGEF domain family, member 1A RASGEF1A NM_145313 3.71E-09 2.83E-08 1.31679 1.22E-09 1.88038 Eukaryotic translation initiation factor 2C, 2 EIF2C2 NM_012154 2.03E-10 7.38E-10 1.32077 8.25E-11 1.68861 chromosome 16 open reading frame 45 C16orf45 NM_033201 1.74E-09 7.17E-10 1.32669 6.06E-09 1.04285 putative insulin-like growth factor II associated protein LOC492304 NM_001007139 0.000482833 0.000494135 1.32995 0.000312429 1.41863 lysyl oxidase-like 2 LOXL2 NM_002318 8.47E-10 9.21E-10 1.33353 6.89E-10 1.37778 cytochrome P450, family 3, subfamily A, polypeptide 5 CYP3A5 NM_000777 5.31E-06 4.30E-06 1.33747 5.17E-06 1.30816 Dedicator of cytokinesis 2 DOCK2 NM_004946 8.11E-09 7.17E-09 1.33849 7.93E-09 1.3234 CDNA clone IMAGE:4794726 ------0.000327509 0.000175583 1.34209 0.000451038 1.17794 GTP cyclohydrolase 1 (dopa-responsive dystonia) GCH1 NM_000161 /// NM_0010240249.02E-07 /// NM_0010240703.36E-07 ///1.34539 NM_0010240713.81E-06 1.01003 dual specificity phosphatase 6 DUSP6 NM_001946 /// NM_0226528.77E-06 6.47E-06 1.34783 9.36E-06 1.28846 NHS-like 1 NHSL1 XM_496826 1.67E-07 1.72E-07 1.3512 1.37E-07 1.3868 CDNA clone IMAGE:5276760 ------6.53E-05 0.000187488 1.35705 2.60E-05 1.75491 aldehyde dehydrogenase 3 family, memberA1 ALDH3A1 NM_000691 4.84E-10 1.02E-09 1.3582 2.51E-10 1.58883 metallothionein 1F (functional) MT1F NM_005949 1.99E-09 2.90E-09 1.36165 1.28E-09 1.49258 brain expressed X-linked 2 /// brain expressed X-linked 2 BEX2 NM_032621 4.84E-07 6.59E-07 1.36439 3.13E-07 1.48847 Supplemental Table 2: Genes that show altered expression in A549 cells in the presence of exogenously added let-7

phospholipase C-like 2 PLCL2 NM_015184 1.07E-05 5.45E-06 1.36544 1.95E-05 1.16757 SAR1 gene homolog B (S. cerevisiae) SAR1B NM_001033503 /// 1.09E-09NM_016103 5.63E-09 1.36634 3.94E-10 1.84366 tetraspanin 5 /// tetraspanin 5 TSPAN5 NM_005723 1.80E-08 2.00E-08 1.3684 1.41E-08 1.42342 metallothionein 2A MT2A NM_005953 4.01E-10 7.57E-10 1.36989 2.20E-10 1.57344 Transcribed locus ------4.23E-06 1.98E-06 1.37108 9.44E-06 1.13576 Transcribed locus ------2.76E-06 3.12E-06 1.37421 2.00E-06 1.44925 prickle-like 1 (Drosophila) PRICKLE1 NM_153026 1.11E-06 2.11E-06 1.37447 5.75E-07 1.60252 decay accelerating factor for complement (CD55, Cromer blood group system)DAF NM_000574 1.27E-10 1.51E-10 1.3753 9.67E-11 1.44585 transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)TFAP2C NM_003222 3.31E-09 5.92E-09 1.37588 1.86E-09 1.56784 collagen, type V, alpha 1 COL5A1 NM_000093 6.63E-06 5.17E-06 1.37921 6.70E-06 1.33671 hepatic leukemia factor HLF NM_002126 1.74E-08 1.19E-08 1.38105 2.28E-08 1.28308 SAR1 gene homolog B (S. cerevisiae) SAR1B NM_001033503 /// 1.79E-07NM_016103 1.91E-06 1.38394 5.45E-08 2.09354 sodium channel, voltage-gated, type I, beta SCN1B NM_001037 /// NM_1990371.36E-08 1.85E-08 1.38401 9.17E-09 1.49852 chromosome 6 open reading frame 141 C6orf141 NM_153344 4.81E-07 3.19E-07 1.38465 6.35E-07 1.27766 hypothetical protein LOC150759 LOC150759 XM_498456 /// XM_4995852.54E-08 3.53E-08 1.38519 1.67E-08 1.50892 Eukaryotic translation initiation factor 2C, 2 EIF2C2 NM_012154 4.43E-07 2.31E-06 1.38574 1.56E-07 1.89993 tetraspanin 5 TSPAN5 NM_005723 1.05E-07 4.02E-08 1.38668 4.32E-07 1.05454 ------2.61E-05 8.95E-06 1.3888 0.000127846 0.990585 decay accelerating factor for complement (CD55, Cromer blood group system)DAF NM_000574 2.81E-10 3.10E-10 1.39347 2.26E-10 1.44402 decay accelerating factor for complement (CD55, Cromer blood group system)DAF NM_000574 8.43E-09 1.07E-08 1.39529 5.99E-09 1.48938 influenza virus NS1A binding protein IVNS1ABP NM_006469 /// NM_0163891.73E-09 9.22E-09 1.39653 6.21E-10 1.89317 chromosome X open reading frame 45 CXorf45 NM_024810 1.81E-07 5.34E-07 1.3967 7.78E-08 1.7457 epithelial protein lost in neoplasm beta EPLIN NM_016357 2.10E-06 7.57E-07 1.39713 9.73E-06 1.02924 dedicator of cytokinesis 9 DOCK9 NM_015296 1.11E-08 7.28E-08 1.39817 3.76E-09 1.95882 Ras-like without CAAX 1 RIT1 NM_006912 2.43E-09 3.97E-09 1.40255 1.45E-09 1.57103 SH3-domain GRB2-like pseudogene 2 SH3GLP2 --- 1.33E-09 7.10E-10 1.40354 2.61E-09 1.21209 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, etaYWHAH poly NM_003405 1.71E-09 1.03E-08 1.40468 5.92E-10 1.9389 lysosomal trafficking regulator LYST NM_000081 /// NM_0010057362.60E-07 1.41E-07 1.41121 4.69E-07 1.22787 5'-nucleotidase, ecto (CD73) NT5E NM_002526 3.06E-07 1.20E-07 1.41146 1.15E-06 1.08431 influenza virus NS1A binding protein IVNS1ABP NM_006469 /// NM_0163894.80E-09 9.36E-09 1.41799 2.57E-09 1.64085 DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked DDX3Y NM_004660 9.86E-09 6.68E-09 1.41808 1.32E-08 1.31213 insulin-like growth factor binding protein 1 IGFBP1 NM_000596 /// NM_0010130292.36E-07 1.42E-07 1.4228 3.60E-07 1.27792 secretory carrier membrane protein 1 SCAMP1 NM_004866 /// NM_0528221.12E-09 2.00E-08 1.4246 3.20E-10 2.27245 hexokinase 1 HK1 NM_000188 /// NM_0334961.28E-06 /// NM_0334978.66E-07 /// NM_0334981.42672 /// NM_0335001.62E-06 1.32526 solute carrier family 25, member 37 SLC25A37 NM_016612 /// NM_0185794.67E-08 5.01E-08 1.43722 3.75E-08 1.48589 dihydropyrimidinase-like 4 DPYSL4 NM_006426 1.23E-06 7.54E-07 1.43891 1.79E-06 1.29909 KIAA1946 KIAA1946 NM_177454 1.71E-07 1.02E-06 1.44438 5.84E-08 2.01384 claudin 3 CLDN3 NM_001306 4.22E-07 7.18E-07 1.45395 2.36E-07 1.65495 zinc finger, DHHC-type containing 20 ZDHHC20 NM_153251 1.95E-08 2.92E-08 1.45875 1.22E-08 1.61091 KIAA1462 KIAA1462 XM_166132 1.32E-07 4.55E-08 1.45919 7.62E-07 1.05316 GLI pathogenesis-related 1 (glioma) GLIPR1 NM_006851 2.52E-06 8.56E-07 1.46088 1.45E-05 1.0397 superoxide dismutase 2, mitochondrial SOD2 NM_000636 /// NM_0010244651.58E-08 /// NM_0010244666.51E-09 1.47238 5.42E-08 1.15685 keratin 19 KRT19 NM_002276 4.65E-09 5.84E-09 1.47357 3.33E-09 1.57039 nanos homolog 1 (Drosophila) NANOS1 NM_001009553 /// 6.61E-08NM_199461 1.26E-07 1.49326 3.52E-08 1.72905 Transcribed locus ------3.94E-07 2.76E-07 1.49496 4.84E-07 1.40039 solute carrier family 16 (monocarboxylic acid transporters), member 2 SLC16A2 NM_006517 1.75E-08 1.22E-08 1.4961 2.25E-08 1.39506 cathepsin S CTSS NM_004079 0.00115034 0.000523381 1.50489 0.00190222 1.24001 ------9.09E-09 1.19E-08 1.50628 6.30E-09 1.61833 Hypothetical protein FLJ14681 FLJ14681 NM_032824 4.38E-10 4.31E-09 1.50824 1.37E-10 2.22286 sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) SPOCK NM_004598 6.20E-08 7.17E-08 1.50944 4.66E-08 1.58568 CUB domain containing protein 1 CDCP1 NM_022842 /// NM_1781817.83E-07 4.76E-07 1.50953 1.16E-06 1.36027 zinc finger CCCH-type containing 12C ZC3H12C XM_370654 2.34E-06 7.92E-07 1.51581 1.37E-05 1.07624 KIAA1462 KIAA1462 XM_166132 2.30E-11 1.06E-11 1.51663 6.16E-11 1.24612 ADP-ribosylation factor-like 7 ARL7 NM_005737 3.07E-11 9.06E-12 1.51891 4.42E-10 0.98322 hypothetical gene supported by BX647608 LOC399959 XM_378316 8.81E-07 4.81E-07 1.51939 1.54E-06 1.32458 diacylglycerol kinase, alpha 80kDa DGKA NM_001345 /// NM_2014442.45E-07 /// NM_2014453.44E-07 /// NM_2015541.523 1.57E-07 1.66798 FERM domain containing 6 FRMD6 NM_152330 2.63E-08 2.36E-08 1.52377 2.50E-08 1.51404 hect domain and RLD 4 HERC4 NM_001017972 /// 3.84E-09NM_015601 /// NM_0220791.43E-08 1.52411 1.54E-09 1.95988 Supplemental Table 2: Genes that show altered expression in A549 cells in the presence of exogenously added let-7

collagen, type VI, alpha 1 COL6A1 NM_001848 2.51E-08 1.71E-08 1.52867 3.34E-08 1.41584 KIAA1462 KIAA1462 XM_166132 2.75E-05 1.13E-05 1.53097 7.55E-05 1.20475 metallothionein 1X MT1X NM_005952 4.97E-11 9.05E-11 1.5405 2.80E-11 1.75616 Spermidine/spermine N1-acetyltransferase SAT NM_002970 1.13E-11 8.06E-12 1.54978 1.45E-11 1.45119 keratin 15 KRT15 NM_002275 3.72E-09 2.99E-09 1.55268 4.07E-09 1.49973 epithelial membrane protein 2 EMP2 NM_001424 2.11E-09 1.67E-08 1.55441 6.86E-10 2.22991 membrane-associated ring finger (C3HC4) 4 5-Mar NM_020814 0.000101334 3.36E-05 1.55568 0.000519702 1.07838 Similar to hypothetical protein LOC231503 --- XM_496707 1.49E-05 9.41E-06 1.55606 1.90E-05 1.42646 mucin 5, subtype B, tracheobronchial MUC5B XM_039877 0.000370837 0.000516179 1.5586 0.000196349 1.78309 reversion-inducing-cysteine-rich protein with kazal motifs RECK NM_021111 1.19E-06 3.85E-07 1.56018 8.76E-06 1.07561 GLI pathogenesis-related 1 (glioma) GLIPR1 NM_006851 8.94E-08 3.59E-08 1.56349 3.19E-07 1.21564 potassium large conductance calcium-activated channel, subfamily M, alpha memberKCNMA1 NM_001014797 /// 6.95E-09NM_002247 4.23E-09 1.56393 1.09E-08 1.40467 EGF-like-domain, multiple 3 EGFL3 XM_031401 2.37E-08 3.21E-08 1.56433 1.59E-08 1.69414 latent transforming growth factor beta binding protein 4 LTBP4 NM_003573 2.33E-06 2.46E-06 1.56732 1.80E-06 1.62672 collagen, type XII, alpha 1 COL12A1 NM_004370 /// NM_0806458.38E-06 6.97E-06 1.5769 7.84E-06 1.55436 A kinase (PRKA) anchor protein 2 /// PALM2-AKAP2 protein AKAP2 /// PALM2-NM_001004065AKAP2 /// 5.33E-07NM_007203 /// NM_1471504.73E-07 1.59286 4.96E-07 1.58398 spermidine/spermine N1-acetyltransferase SAT NM_002970 3.34E-10 3.79E-10 1.59332 2.62E-10 1.66103 butyrophilin-like 9 BTNL9 NM_152547 8.61E-08 1.16E-07 1.60656 5.73E-08 1.74249 PALM2-AKAP2 protein PALM2-AKAP2 NM_007203 /// NM_1471501.30E-08 1.07E-08 1.60875 1.38E-08 1.56251 lipoma HMGIC fusion partner LHFP NM_005780 1.09E-06 3.34E-07 1.61089 1.02E-05 1.07285 aquaporin 3 AQP3 NM_004925 9.68E-10 1.13E-09 1.61112 7.39E-10 1.69032 suppressor of cytokine signaling 3 SOCS3 NM_003955 4.84E-05 1.64E-05 1.614 0.000238049 1.14071 UL16 binding protein 2 ULBP2 NM_025217 2.79E-08 4.09E-08 1.61618 1.77E-08 1.77809 forkhead box Q1 FOXQ1 NM_033260 2.39E-08 1.36E-08 1.63008 4.10E-08 1.438 EGF-like repeats and discoidin I-like domains 3 EDIL3 NM_005711 1.86E-08 3.27E-08 1.63128 1.05E-08 1.85563 FERM domain containing 6 FRMD6 NM_152330 8.45E-07 6.67E-07 1.63664 8.86E-07 1.58299 quaking homolog, KH domain RNA binding (mouse) QKI NM_006775 /// NM_2068538.49E-09 /// NM_2068541.83E-08 /// NM_2068551.63701 4.29E-09 1.92987 sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and SEMA4Bshort cyt NM_020210 /// NM_1989251.86E-08 9.16E-09 1.63863 4.21E-08 1.37735 Transcribed locus ------5.92E-08 8.70E-07 1.64081 1.72E-08 2.57971 chromosome 6 open reading frame 141 C6orf141 NM_153344 4.46E-07 2.16E-07 1.64749 9.91E-07 1.37917 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type SERPINE2 NM_006216 3.74E-11 4.03E-11 1.65718 3.09E-11 1.70686 four and a half LIM domains 1 FHL1 NM_001449 3.49E-07 4.50E-07 1.66356 2.36E-07 1.79362 Family with sequence similarity 20, member C ITPR2 NM_002223 5.97E-10 2.28E-10 1.66639 2.61E-09 1.26675 neuron navigator 3 NAV3 NM_014903 1.20E-08 4.57E-09 1.66889 5.23E-08 1.26489 alanyl (membrane) (aminopeptidase N, aminopeptidase M, microsomalANPEP NM_001150 3.21E-06 6.84E-06 1.67009 1.54E-06 1.99677 coagulation factor II (thrombin) receptor F2R NM_001992 2.13E-08 3.44E-08 1.67341 1.26E-08 1.87578 ELOVL family member 7, elongation of long chain fatty acids (yeast) ELOVL7 NM_024930 5.94E-10 2.34E-09 1.67608 2.35E-10 2.16988 CUB domain containing protein 1 CDCP1 NM_022842 /// NM_1781813.88E-06 2.84E-06 1.68811 4.29E-06 1.60659 hyaluronan synthase 3 HAS3 NM_005329 /// NM_1386125.18E-09 1.57E-09 1.71061 5.99E-08 1.13131 solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter SLC1A1 NM_004170 3.75E-07 3.16E-07 1.71198 3.71E-07 1.68053 Full-length cDNA clone CS0DM011YC22 of Fetal liver of Homo sapiens (human)------1.08E-10 2.36E-10 1.71206 5.54E-11 2.01317 integrin, beta 4 ITGB4 NM_000213 /// NM_0010056193.84E-08 /// NM_0010057317.76E-08 1.71945 1.99E-08 2.00869 aldehyde oxidase 1 AOX1 NM_001159 2.95E-07 2.33E-07 1.73381 3.13E-07 1.67583 delta-notch-like EGF repeat-containing transmembrane DNER NM_139072 2.44E-07 1.00E-07 1.73451 7.96E-07 1.36376 CDNA clone IMAGE:5531727 ------1.48E-10 8.45E-11 1.7364 2.64E-10 1.52819 PALM2-AKAP2 protein PALM2-AKAP2 NM_007203 /// NM_1471506.03E-08 4.25E-08 1.74924 7.57E-08 1.63711 follistatin-like 1 FSTL1 NM_007085 8.34E-11 6.63E-11 1.7642 9.39E-11 1.69684 Xanthine dehydrogenase XDH NM_000379 2.69E-10 2.36E-10 1.76817 2.70E-10 1.74144 stanniocalcin 1 STC1 NM_003155 3.56E-06 1.05E-06 1.76834 4.11E-05 1.13127 interferon epsilon 1 IFNE1 NM_176891 9.99E-09 7.76E-09 1.76904 1.13E-08 1.69578 Supplemental Table 2: Genes that show altered expression in A549 cells in the presence of exogenously added let-7

Interleukin 6 signal transducer (gp130, oncostatin M receptor) IL6ST NM_002184 /// NM_1757673.64E-11 4.73E-11 1.77351 2.58E-11 1.89765 epithelial membrane protein 1 EMP1 NM_001423 1.62E-08 1.39E-08 1.77529 1.63E-08 1.74386 plasminogen activator, urokinase PLAU NM_002658 1.02E-11 5.76E-12 1.78303 1.86E-11 1.56425 phospholipid scramblase 4 PLSCR4 NM_020353 1.69E-06 4.70E-07 1.79675 3.39E-05 1.07165 Proline rich Gla (G-carboxyglutamic acid) 4 (transmembrane) PRRG4 NM_024081 1.34E-08 4.35E-09 1.79859 1.04E-07 1.2529 dickkopf homolog 3 (Xenopus laevis) DKK3 NM_001018057 /// 1.02E-09NM_013253 /// NM_0158818.66E-10 1.80661 1.05E-09 1.76854 RAB3B, member RAS oncogene family RAB3B NM_002867 4.79E-07 2.43E-07 1.81582 9.69E-07 1.54493 collagen, type XIII, alpha 1 COL13A1 NM_005203 /// NM_0807987.63E-07 /// NM_0807995.64E-07 /// NM_0808001.82489 /// NM_0808018.64E-07 /// NM_0808021.73583 Interleukin 6 signal transducer (gp130, oncostatin M receptor) IL6ST NM_002184 /// NM_1757674.15E-11 4.87E-11 1.82731 3.19E-11 1.91571 integrin, beta 4 ITGB4 NM_000213 /// NM_0010056194.65E-07 /// NM_0010057317.13E-07 1.82945 2.78E-07 2.04181 hypothetical protein FLJ11259 FLJ11259 NM_018370 2.52E-10 2.28E-10 1.8417 2.44E-10 1.82746 Kruppel-like factor 11 KLF11 NM_003597 1.38E-07 9.54E-08 1.84244 1.75E-07 1.71852 protein kinase C, delta binding protein PRKCDBP NM_145040 5.85E-07 5.58E-07 1.85431 5.06E-07 1.87571 CDNA clone IMAGE:5531727 ------8.12E-11 5.23E-11 1.85836 1.20E-10 1.69321 ring finger protein 182 RNF182 NM_152737 4.84E-09 4.05E-09 1.85883 5.04E-09 1.81362 chromosome 20 open reading frame 100 C20orf100 NM_032883 8.19E-07 4.36E-07 1.88012 1.50E-06 1.62604 guanylate binding protein 3 GBP3 NM_018284 2.07E-08 1.21E-08 1.88408 3.44E-08 1.67244 Doublecortin and CaM kinase-like 1 DCAMKL1 NM_004734 1.36E-06 3.60E-07 1.88547 9.50E-05 0.95444 collagen, type VI, alpha 2 COL6A2 NM_001849 /// NM_0581744.71E-08 /// NM_0581754.82E-08 1.91307 3.94E-08 1.95767 Adipocyte-specific adhesion molecule ASAM NM_024769 1.18E-08 1.12E-08 1.91406 1.07E-08 1.92348 5'-nucleotidase, ecto (CD73) NT5E NM_002526 6.01E-10 1.84E-10 1.92736 6.63E-09 1.28754 coronin, actin binding protein, 2B CORO2B NM_006091 7.53E-07 4.29E-07 1.93408 1.24E-06 1.70816 plasminogen activator, urokinase /// plasminogen activator, urokinase PLAU NM_002658 8.25E-09 5.07E-09 1.95014 1.28E-08 1.75611 arylacetamide deacetylase-like 1 AADACL1 NM_020792 9.21E-08 1.26E-07 1.95246 6.06E-08 2.12372 v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian) MAFF NM_012323 /// NM_1528785.75E-07 1.87E-07 1.98884 4.15E-06 1.38077 Integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor) ITGA2 NM_002203 1.88E-10 7.50E-11 2.01654 7.27E-10 1.56301 regulated in glioma RIG --- 2.95E-06 1.75E-06 2.01717 4.39E-06 1.80672 zinc finger homeobox 1b ZFHX1B NM_014795 7.01E-06 2.47E-06 2.04999 3.35E-05 1.48849 neuron navigator 3 NAV3 NM_014903 6.07E-09 2.02E-09 2.06092 4.32E-08 1.45598 SMAD specific E3 ubiquitin protein ligase 2 SMURF2 NM_022739 3.23E-10 1.39E-10 2.06545 1.01E-09 1.65298 SMAD specific E3 ubiquitin protein ligase 2 SMURF2 NM_022739 5.06E-08 4.78E-08 2.07383 4.56E-08 2.08509 Tissue factor pathway inhibitor 2 TFPI2 NM_006528 5.17E-12 3.74E-12 2.07598 6.54E-12 1.95089 SMAD specific E3 ubiquitin protein ligase 2 SMURF2 NM_022739 1.79E-08 7.73E-09 2.08824 5.42E-08 1.67305 protease, serine, 3 (mesotrypsin) PRSS3 NM_002771 5.68E-10 1.12E-09 2.09012 3.04E-10 2.41982 podocalyxin-like PODXL NM_001018111 /// 4.47E-08NM_005397 2.26E-08 2.09388 9.50E-08 1.77652 chemokine (C-X-C motif) ligand 5 CXCL5 NM_002994 5.74E-08 1.84E-08 2.14924 4.70E-07 1.48021 Homeodomain interacting protein kinase 3 HIPK3 NM_005734 5.88E-11 1.86E-10 2.22681 2.53E-11 2.78312 Fc fragment of IgG binding protein FCGBP NM_003890 1.23E-06 1.76E-06 2.23089 7.63E-07 2.46224 spermidine/spermine N1-acetyltransferase SAT NM_002970 1.01E-12 8.05E-13 2.23331 1.15E-12 2.14648 Stanniocalcin 1 STC1 NM_003155 3.04E-06 8.69E-07 2.26103 4.66E-05 1.39167 protease, serine, 3 (mesotrypsin) PRSS3 NM_002771 1.36E-08 1.84E-08 2.27741 9.11E-09 2.46697 Ankyrin repeat domain 44 LOC91526 NM_153697 1.02E-07 1.30E-07 2.30966 7.05E-08 2.47857 tumor necrosis factor, alpha-induced protein 6 TNFAIP6 NM_007115 5.87E-11 6.60E-11 2.36009 4.66E-11 2.45367 chemokine (C-X-C motif) ligand 3 CXCL3 NM_002090 8.40E-07 3.12E-07 2.36614 3.61E-06 1.77261 dickkopf homolog 3 (Xenopus laevis) DKK3 NM_001018057 /// 6.49E-08NM_013253 /// NM_0158814.26E-08 2.42852 8.93E-08 2.23122 tumor necrosis factor, alpha-induced protein 6 TNFAIP6 NM_007115 1.05E-11 1.15E-11 2.49529 8.53E-12 2.58103 homeodomain interacting protein kinase 3 HIPK3 NM_005734 1.98E-09 3.50E-09 2.56368 1.12E-09 2.91327 Ras-induced senescence 1 RIS1 NM_015444 8.25E-07 5.21E-07 2.57074 1.15E-06 2.34265 SMAD specific E3 ubiquitin protein ligase 2 SMURF2 NM_022739 1.86E-08 1.57E-08 2.57517 1.91E-08 2.51742 chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)CXCL1 NM_001511 8.03E-07 2.42E-07 2.57772 8.77E-06 1.68343 ADP-ribosylation factor 7 ARF7 NM_025047 3.49E-08 1.20E-08 2.61501 2.09E-07 1.88631 aquaporin 3 AQP3 NM_004925 2.87E-09 3.61E-09 2.73363 2.05E-09 2.91371 chemokine (C-X-C motif) ligand 2 CXCL2 NM_002089 8.16E-07 2.28E-07 2.87306 1.67E-05 1.72097 doublecortin and CaM kinase-like 1 DCAMKL1 NM_004734 1.54E-07 4.05E-08 2.93625 1.99E-05 1.41129 chemokine (C-X-C motif) ligand 5 CXCL5 NM_002994 2.92E-05 1.52E-05 3.25073 4.89E-05 2.80598 interleukin 8 IL8 NM_000584 1.42E-08 4.67E-09 3.31935 1.06E-07 2.32458 interleukin 8 IL8 NM_000584 6.83E-09 2.26E-09 3.45134 5.01E-08 2.42736 nidogen 1 NID1 NM_002508 1.83E-10 2.51E-10 3.53123 1.24E-10 3.82257 Supplemental Table 3 Gene Symbol Role RefSeq Transcript ID LCS CDK6 Kinase NM_001259 10 PLAGL2 Transcription factor NM_002657 9 2'-PDE phosphodiesterase NM_177966 5 CDC25A phophatase NM_001789 /// NM_201567 4 DZIP1 RNA binding NM_014934 /// NM_198968 4 OPRS1 Opioid receptor NM_005866 /// NM_147157 /// NM_147158 /// NM_147159 /// NM_147160 4 DLC1 RhoGAP NM_006094 /// NM_024767 /// NM_182643 3 NXT2 Nuclear transport NM_018698 3 E2F6 Transcription factor NM_001952 /// NM_198256 /// NM_198257 /// NM_198258 /// NM_198325 /// NM_212540 3 GPX7 Glutathione peroxidase NM_015696 3 SSR1 Signal sequence receptor NM_003144 3 YAP1 Transcription factor NM_006106 3 ZNF336 Transcription factor NM_022482 3 SOX9 Transcription factor NM_000346 3 RRM2 Ribonucleotide reductase NM_001034 3 STK6 Aurora A kinase NM_003158 /// NM_003600 /// NM_198433 /// NM_198434 /// NM_198435 /// NM_198436 3 FARP1 RhoGEF NM_001001715 /// NM_005766 2 FLJ21986 Unknown NM_024913 2 CBFB Transcription factor NM_001755 /// NM_022845 2 DSCR1 Calcipressin NM_004414 /// NM_203417 /// NM_203418 2 CDCA7 Novel c-Myc-responsive Gene NM_031942 /// NM_145810 2 MAP3K7IP2 kinase adaptor NM_015093 /// NM_145342 1 GTF2I/GTF2IP1 Transcription factor NM_001518 /// NM_032999 /// NM_033000 /// NM_033001 /// NM_033003 /// XR_000285 1 ARID3A Transcription factor NM_005224 1 DMD Dystrophin NM_000109 /// NM_004006 /// NM_004007 /// NM_004009 /// NM_004010 /// NM_004011 1 TUBB NM_178014 NUDT4 NM_019094 /// NM_199040 HRB NM_004504 ID2 NM_002166 ACTB NM_001101 SNX6 NM_021249 /// NM_152233 IF NM_000204 SNX5 NM_014426 /// NM_152227 CAT NM_001752 LIPA NM_000235 RNP24 NM_006815 ARS2 NM_015908 /// NM_182800 SARA1 NM_020150 ALDH5A1 NM_001080 /// NM_170740 HP NM_005143 PAPOLA NM_032632 RRBP1 NM_004587 C20orf59 NM_022082 MGC17943 NM_152261 ITGB1 NM_002211 /// NM_033666 /// NM_033667 /// NM_033668 /// NM_033669 /// NM_133376 GLUD1 NM_005271 CTSC NM_001814 /// NM_148170 MCCC2 NM_022132 TLOC1 NM_003262 ACTB NM_001101 GORASP2 NM_015530 PNN NM_002687 EIF5 NM_001969 /// NM_183004 NFIL3 NM_005384 C9orf64 NM_032307 RAB10 NM_016131 ARHGAP18 NM_033515 GRSF1 NM_002092 SNRPB2 NM_003092 /// NM_198220 FLJ21924 NM_024774 CANX NM_001746 DENR NM_003677 RAB14 NM_016322 GRLF1 NM_004491 /// NM_024342 PCYOX1 NM_016297 DENR NM_003677 HERPUD1 NM_014685 SLC35F5 NM_025181 CHP NM_007236 PS1TP4 --- CGI-48 NM_016001 LYRIC NM_178812 MAT2A NM_005911 DKFZP586L0724 NM_015462 PPAP2B NM_003713 /// NM_177414 C20orf36 NM_018257 QKI NM_006775 /// NM_206853 /// NM_206854 /// NM_206855 SC4MOL NM_006745 NM_018243 AGPS NM_003659 PNN NM_002687 LYAR NM_017816 VAMP3 NM_004781 C3 NM_000064 MAPRE1 NM_012325 G3BP NM_005754 /// NM_198395 KBTBD6 NM_152903 SMAD4 NM_005359 DNCLI2 NM_006141 SERP1 NM_014445 HMGCS1 NM_002130 SARA2 NM_016103 AK3 NM_001005353 /// NM_013410 /// NM_203464 IL6R NM_000565 /// NM_181359 XPOT NM_007235 MLLT4 NM_005936 ANP32E NM_030920 PIGW NM_178517 LAMP2 NM_002294 /// NM_013995 CDC16 NM_003903 CDW92 NM_022109 /// NM_080546 PNN NM_002687 PPP2R5E NM_006246 TIA1 NM_022037 /// NM_022173 NDFIP1 NM_030571 PPP4R1 NM_005134 PPIF NM_005729 SRP72 NM_006947 SRP68 NM_014230 ITGA6 NM_000210 LOC163590 NM_145034 CKAP4 NM_006825 MINA NM_032778 /// NM_153182 FLJ10826 NM_018233 AHCYL1 NM_006621 /// NM_014121 PLOD2 NM_000935 /// NM_182943 BRP44L NM_016098 PGM2 NM_018290 KIAA0650 --- PRPF4 NM_004697 IFNGR1 NM_000416 NR1H4 NM_005123 MGC15397 NM_080652 MGC33302 NM_152778 GIPC2 NM_017655 ANLN NM_018685 ELOVL1 NM_016031 /// NM_022821 CTSH NM_004390 /// NM_148979 OBRGRP NM_017526 CYP51A1 NM_000786 C6orf96 NM_017909 SYNCRIP NM_006372 SPR NM_003124 SRPRB NM_021203 SDC2 NM_002998 MGC15396 NM_052855 TOMM70A NM_014820 USP14 NM_005151 ME2 NM_002396 FBXO2 NM_012168 SLCO4C1 NM_018515 /// NM_180991 LOC145786 --- LOC201194 --- SKP2 NM_005983 /// NM_032637 MGC14289 NM_080660 PAH NM_000277 MGC15397 NM_080652 CEBPA NM_004364 NR1H4 NM_005123 DGAT1 NM_012079 KLHL14 --- C13orf23 NM_025138 /// NM_170719 ERO1L NM_014584 IL6R NM_000565 /// NM_181359 SLC13A5 NM_177550 MGC12916 --- C9orf41 NM_152420 MOV10 NM_020963 C14orf46 --- LOC163782 NM_181712 MAL2 NM_052886 CPN2 --- CSNK2A1 NM_001895 /// NM_177559 /// NM_177560 MGC12916 --- RAMP NM_016448 MSH6 NM_000179 SGKL NM_013257 /// NM_170709 SLC5A9 --- SLC30A10 NM_001004433 /// NM_018713 NAP1L1 NM_004537 /// NM_022348 /// NM_139207 AGXT2L1 NM_031279 Pfs2 NM_016095 CCNA2 NM_001237 MCAM NM_006500 IL6R NM_000565 /// NM_181359 GSTA1 NM_145740

Suppl. Table 4. The most affected GO categories after let-7 over-expression in the time course analysis of HepG2 cells. # # in % in the GO ID genes GO description category P value category GO:0000278 19 mitotic cell cycle 192 5.5E-08 10% GO:0051301 15 cell division 135 3.1E-07 11% GO:0000279 15 M phase 165 3.8E-06 9% GO:0007088 6 regulation of mitosis 34 8.9E-05 18% GO:0016126 5 sterol biosynthesis 24 1.5E-04 21% GO:0005525 16 GTP binding 262 2.0E-04 6% GO:0006260 11 DNA replication 138 2.2E-04 8% GO:0051325 8 interphase 76 2.5E-04 11%

* P values were calculated with hypergeometric tests to determine whether there is a significant enrichment of affected genes in a GO category when compared to all genes represented on the arrays.

36