DOCSLIB.ORG

Antibacterial Activity of Extract and Essential Oil of Ziziphora Tenuior L

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Antibacterial Activity of Extract and Essential Oil of Ziziphora Tenuior L Medical Laboratory Journal,Nov, Dec 2016; Vol 10: No 6 Original Article Antibacterial Activity of Ziziphora Tenuior Lam. Extract and Essential Oil against Bacteria Isolated from Urogenital Tract Infections ABSTRACT Younes Anzabi (PhD) Background and Objectives: Many species of Ziziphora have been used in Department of Pathobiology, Tabriz Branch, Islamic Azad University, traditional medicine in the Azarbaijan region, northwest of Iran. This study aimed to Tabriz, Iran determine antibacterial activity of essential oil and methanol extract of Ziziphora tenuior on Arash Khaki (PhD) some pathogenic bacteria isolated from urogenital tract infections. Department of Pathobiology, Tabriz Methods: The essential oil and methanol extract of Z. tenuior were obtained by Branch, Islamic Azad University, Tabriz, Iran Clevenger and maceration methods. Under sterile conditions, the required amount of urine Corresponding author: Arash Khaki was taken from patients referred to a number of clinics in Tabriz during 2014. After Tel: +984136372274 identification of the isolates using standard microbiological methods, antimicrobial effects of E-mail: [email protected] the essential oil and methanol extract of the plant on the isolates were evaluated by Address: Tabriz Branch, Islamic determining minimum inhibitory concentration (MIC), minimum bactericidal concentration, Azad University, Tabriz, Iran and antibiogram test. Results: The MIC for essential oil of Z. tenuior was 250µg/mL for most Gram- Received : 15 Nov 2014 negative bacteria except Pseudomonas aeruginosa. Moreover, the MIC for Staphylococcus Revised: 01 Dec 2014 aureus was 250µg/mL and 500µg/mL for other strains of Staphylococcus. Accepted: 16 Dec 2014 Conclusion: Comparison of the inhibitory and bactericidal effects of the essential oil and methanol extract of Z. tenuior showed that the essential oil is able to inhibit growth of the bacteria tested even in low concentrations. Further studies are required in this regard using animal models. Keywords: Antibacterial Agents, Ziziphora tenuior, Plant Extracts, Urinary Tract Infection. This paper should be cited as: Anzabi Y, Khaki A[Antibacterial Activity of Ziziphora Tenuior Lam. Extract and Essential Oil against Bacteria Isolated from Urogenital Tract Infections] . mljgoums. 2017; 10(6): 54-59 55/ Anzabi and colleagues INTRODUCTION Plant extracts and their constituents have stored at 4 °C. For production of essential some known antibacterial effects (1). oil, Clevenger apparatus (Schott DURAN- Ziziphora tenuior from the Ziziphora genus Germany) and water distillation were used (Lamiaceae family) and breed of mint is a 5- (6-8). Bacterial strains were isolated from 15 cm tall herbaceous plant with a one-year patients with urinary tract infections referred lifetime, short stems and thin sharp leaves. to a number of clinics in Tabriz in 2014. The This plant grows wild in several areas of isolates included nine species. The essential Iran including the mountainous regions of oil and methanol extract of the plant were Azerbaijan provinces, especially in mountains individually tested against three Gram-positive of Tabriz. Four species of this plant including (Staphylococcus aureus, Staphylococcus Ziziphora clinopodioides, Ziziphora capitata, epidermidis, Staphylococcus saprophyticus) Ziziphora persica and Z. tenuior have been and six Gram-negative (Escherichia coli, identified in Iran. Mucolytic, carminative Klebsiella pneumonia, Proteus vulgaris, and stomach reinforcement activities are Enterobacter aerogenes, Citrobacter frundii among the healing effects of this plant. In and Pseudomonas aeroginosa) bacteria. A 24- some areas, the mixture of its powdered hour culture of each bacterium was used for leaves with honey is used to treat dysentery. It Preparation of bacterial suspension. First, 24 is also used as an antiseptic cold remedy, and hours before testing, the stored cultures were for treatment of stomach disorders (2, 3). In inoculated into Brain Heart Infusion agar different regions, the plant’s powder is (Merck-Germany) and incubated at 37 °C for used as garnish on yogurt and dairy products 24h. Colonies were washed with normal (4). Despite the wide range of traditional saline solution and bacterial suspensions applications of this plant for treatment of many were diluted with normal saline to reach infectious diseases, a systematic study has not turbidity equal to the 0.5 McFarland been yet performed on the antibacterial effects standards (9, 10). Disk diffusion method was of Z. tenuior on pathogenic bacteria. used to evaluate the antibacterial effects of Therefore, this in vitro study aimed to the essential oil and methanol extract of Z. determine the antibacterial activity of essential tenuior. Disks containing the extract were oil and methanol extract of Z. tenuior prepared from sterile blank disks collected from the hills around the city of manufactured by Padtan Teb Co. (Iran). The Tabriz in East Azerbaijan Province against blank disks were placed in tubes containing some pathogenic bacteria isolated from essential oil and methanol extract of Z. tenuior urinary tract infections. for 30-50 minutes. Following complete absorption, the disks were kept at 44-45 °C MATERIAL AND METHODS until completely dried (11). Then, 100 μl of Aerial parts of Z. tenuior in blooming status prepared suspension from each isolated were collected from hills located 15-20 km far bacterium was cultured separately on the from Tabriz (1700-1800m) in the East surface of Muller Hinton agar (Merck- Azerbaijan Province. A voucher specimen of Germany). The disks impregnated with the the plant was identified and preserved at the essential oil and methanol extracts of Z. Herbarium of Department of Food Hygiene at tenuior were placed with certain distance from Islamic Azad University of Tabriz, Iran. The each other and from the edge of the plate on samples were shade-dried, powdered and the surface of the medium using sterile stored at 4 ºC until in vitro testing (5). For forceps. The disks were fixed with little Preparation of plant extraction, in a specialized pressure and the plates were incubated for laboratory at the Department of Pathobiology, 24h at 37 °C. Antibacterial activity was one gram of the collected parts mixed with assessed by measuring the diameter of 100ml of 80% methanol (Merck-Germany) inhibition zone around the disks. The was extracted by maceration. The Extracts experiment was repeated three times for each were filtered by Whatman No.1 filter paper bacterium. The mean inhibition zone diameter (chm F2042-Spain). The filtrates obtained obtained from the triplicates was used as the were dried in a rotary evaporator (Stuart final diameter (12, 13). Moreover, standard RE300-England) at 40 °C. The extract was antibiotic ampicillin disk (10µg) was used as Medical Laboratory Journal, Nov, Dec 2016; Vol 10: No 6 56/ Antibacterial Activity of. positive control. Determination of minimum antibacterial effects of essential oil and inhibitory concentration (MIC) and minimum methanol extract of Z. tenuior against all bactericidal concentration (MBC): Broth bacteria except for E. coli, C. frundii and E. microdilution method was used for this aerogenes. The highest antibacterial effect purpose. In order to determine the MIC for was observed against K. pneumoniae. essential oil and methanol extract of Z. Compared with the positive control tenuior, a series of 10 test tubes was used. (ampicillin), the essential oil and methanol Eight tubes were used for testing different extract showed higher antibacterial activity concentrations of essential oil and methanol in most cases. This effect was more evident extract (according to Tables 2-5) and two in the case of essential oil. The MIC of tubes were used as controls. All test tubes essential oil for Z. tenuior was ≤125 mcg/ml were incubated at 37 °C for 24 hours. After for most Gram-negative bacteria (Table 2). the incubation period, the turbidity of tubes In addition, the MBC of essential oil for was studied for bacterial growth. This bacterial isolates tested was equal to the MIC method was repeated three times for each value. On the other hand, the MIC for Gram- bacterium. The lowest concentration of positive bacteria including S. aureus was 250 extract and essential oil that inhibited mcg/ml, and 500 mcg/ml for other bacterial growth was considered as the MIC. Staphylococcus strains (Table 2). The results A sample from the lowest concentration of also showed that the essential oil and the methanol extract and essential oil at methanol extract of Z. tenuior have no effect which no bacterial growth was observed on P. aeroginosa at the tested concentrations. was cultured by pure plate method to Among the Gram-positive bacteria, S. aureus determine the MBC (14, 15). was the most sensitive to the concentrations Tukey's test was used for comparing the of Z. tenuior essential oil tested in our study. samples. ANOVA with equal frequency was Among the six Gram-negative bacteria, K. used to determine significant differences pneumoniae showed more sensitivity to the between the means. Descriptive statistics was essential oil compared to the other species. used to compare MBC and MIC of plant The extract had inhibitory and bactericidal extract and essential oil. effects at all concentrations tested against all bacterial isolates except P. aeruginosa RESULTS (Table 2). The MBC of extract for most At 95% confidence level, there isolates was equal to their MIC values was significant difference between the (Table 3). Table 1- in vitro antibacterial activity of essential oil (10 µl/disk) and methanol extract (10 µl/disk) of Z. tenuior compared to ampicillin (10µg/disk) as the standard antibiotic Essential oil of Z. tenuior Methanol extract of Z. Ampicillin tenuior Mean diameter of inhibition Mean diameter of Mean diameter of Bacterial isolates zone (mm) ±SD inhibition zone (mm) ±SD inhibition zone (mm) ±SD S. aureus 22±0.1 19±0.1 13± 0.1 S. epidermidis 20±0.1 19.5±0.2 14±0.1 S. saprophyticus 21±0.1 19±0.1 14±0.1 E. coli 21±0.1 20±0.1 13±0.1 K. pneumonia 29±0.1 26±0.1 19±0.1 P.
Load more

Recommended publications
  • ﺑﺮﺭﺳﻰ ﺑﻴﻮﺳﻴﺴﺘﻤﺎﺗﻴﮑﻰ ﺟﻨﺲ Ziziphora اﺯ ﺧﺎﻧﻮاﺩﻩ Lamiaceae ﺩﺭ اﻳﺮاﻥ
    ﺑﺮﺭﺳﻰ ﺑﻴﻮﺳﻴﺴﺘﻤﺎﺗﻴﮑﻰ ﺟﻨﺲ Ziziphora اﺯ ﺧﺎﻧﻮاﺩﻩ Lamiaceae ﺩﺭ اﻳﺮاﻥ ﻧﺎﻡ ﺩاﻧﺸﺠﻮ: ﺭﻗﻴﻪ ﺟﻬﺎﻧﺪﻳﺪﻩ اﺳﺘﺎﺩ ﺭاﻫﻨﻤﺎ: ﻣﺮﻳﻢ ﮐﺸﺎﻭﺭﺯﻯ اﺳﺘﺎﺩ ﻣﺸﺎﻭﺭ: ﻣﻬﻮﺵ ﺳﻴﻔﻌﻠﻰ ﺩاﻧﺸﮑﺪﻩ ﻋﻠﻮﻡ ﭘﺎﻳﻪ ﺗﺎﺭﻳﺦ ﺩﻓﺎﻉ: ۱۳۸۷ ﺩاﻧﺸﮕﺎﻩ اﻟﺰﻫﺮا داﻧﺸﮕﺎه اﻟﺰﻫﺮا (س) داﻧﺸﻜﺪه ﻋﻠﻮم ﭘﺎﻳﻪ ﭘﺎﻳﺎن ﻧﺎﻣﻪ ﺟﻬﺖ اﺧﺬ درﺟﻪ ﻛﺎرﺷﻨﺎﺳﻲ ارﺷﺪ رﺷﺘﻪ زﻳﺴﺖ ﺷﻨﺎﺳﻲ ﻋﻠﻢ ﮔﻴﺎﻫﻲ- ﮔﺮاﻳﺶ ﺳﻴﺴﺘﻤﺎﺗﻴﻚ ﮔﻴﺎﻫﻲ ﻋﻨﻮان: ﺑﺮرﺳﻲ ﺑﻴﻮﺳﻴﺴﺘﻤﺎﺗﻴﻜﻲ ﺟﻨﺲ Ziziphora از ﺧﺎﻧﻮاده Lamiaceae در اﻳﺮان اﺳﺎﺗﻴﺪ راﻫﻨﻤﺎ: دﻛﺘﺮ ﻣﺮﻳﻢ ﻛﺸﺎورزي دﻛﺘﺮ زﻫﺮا ﻧﺎﻇﻢ ﺑﻜﺎﻳﻲ اﺳﺘﺎد ﻣﺸﺎور: ﻣﻬﻨﺪس ﻣﻬﻮش ﺳﻴﻔﻌﻠﻲ داﻧﺸﺠﻮ: رﻗﻴﻪ ﺟﻬﺎﻧﺪﻳﺪه ﺗﺎرﻳﺦ دﻓﺎع: 3ﺑﻬﻤﻦ ﻣﺎه 87 ﺟﻨﺲ .Ziziphora L ﺷﺎﻣﻞ ﮔﻴﺎﻫﺎﻧﻲ ﻳﻚﺳﺎﻟﻪ و ﭼﻨﺪﺳﺎﻟﻪ از ﺧﺎﻧﻮاده ﻧﻌﻨﺎع ﻣﺘﻌﻠﻖ ﺑﻪ زﻳﺮﺧﺎﻧﻮاده Nepetoideae ﻣﻲﺑﺎﺷﺪ. اﻳﻦ ﺟﻨﺲ ﻛﻪ ﺷﺎﻣﻞ ﺗﻘﺮﻳﺒﺎ 40 ﮔﻮﻧﻪ در ﻣﺪﻳﺘﺮاﻧﻪ، ﻣﻨﻄﻘﻪ اﻳﺮان و ﺗﻮران و ﺑﻪ وﻳﮋه در ﻣﺰﻛﺰ آﺳﻴﺎﺳﺖ در اﻳﺮان ﺷﺎﻣﻞ 40 ﮔﻮﻧﻪ ﻣﻲﺑﺎﺷﺪ: 4) Z. tenuior 3) Z. persica Bunge Z. clinopodioides Lam. 2) Z.capitat L. 1) L. اﻋﻀﺎي اﻳﻦ ﺟﻨﺲ ﺗﻘﺮﻳﺒﺎ در ﺗﻤﺎم ﻧﻮاﺣﻲ اﻳﺮان ﮔﺴﺘﺮدهاﻧﺪ. در اﻳﻦ ﭘﮋوﻫﺶ 37 واﺣﺪ ﺟﻤﻌﻴﺘﻲ از اﻋﻀﺎي ﮔﻮﻧﻪﻫﺎي Ziziphora از ﻧﻈﺮ ﺳﺎﺧﺘﺎر ﺗﺸﺮﻳﺤﻲ (ﺑﺮش ﻋﺮﺿﻲ و ﺳﺎﺧﺘﻤﺎن ﺑﺸﺮه ﭘﺸﺘﻲ)، رﻳﺨﺖ ﺷﻨﺎﺳﻲ، ﻣﻴﻜﺮوﻣﻮرﻓﻮﻟﻮژي و ﺷﻤﺎرش ﻛﺮوﻣﻮزوﻣﻲ ﻣﻮرد ﺑﺮرﺳﻲ ﻗﺮار ﮔﺮﻓﺘﻪاﻧﺪ. در ﺑﺨﺶ رﻳﺨﺖﺷﻨﺎﺳﻲ ﺻﻔﺎت ﻛﻤﻲ و ﻛﻴﻔﻲ ﺑﺨﺶﻫﺎي روﻳﺸﻲ و زاﻳﺸﻲ ﻫﺮ ﺟﻤﻌﻴﺖ ﻣﻄﺎﻟﻌﻪ ﺷﺪ. ﺗﺤﻠﻴﻞ آﻣﺎري ﺑﻪ ﺻﻮرت آﻧﺎﻟﻴﺰ ﭼﻨﺪ ﻣﺘﻐﻴﺮه ﺑﺎ اﺳﺘﻔﺎده از ﻧﺮماﻓﺰار .SPSS ver15. 1 ﺻﻮرت ﮔﺮﻓﺖ. در اﻳﻦ ﺟﻨﺲ ﺑﺎ اﺳﺘﻔﺎده از آﻧﺎﻟﻴﺰ ﻓﺎﻛﺘﻮر ﻣﺸﺨﺺ ﺷﺪ ﻛﻪ ﺻﻔﺎت ﺑﺴﺎك، ﺷﻜﻞ ﺑﺮگ ﻫﻤﺮاه ﮔﻞآذﻳﻦ، ﺷﻜﻞ ﮔﻞآذﻳﻦ، ﻋﺮض ﺑﺮگ ﻫﻤﺮاه ﮔﻞآذﻳﻦ، ﺑﺮگﻫﺎي ﭘﺎﻳﻪ ﮔﻞآذﻳﻦ، ﻃﻮل ﻛﺎﺳﻪ، ﻃﻮل ﺑﺮگ ﻫﻤﺮاه ﮔﻞآذﻳﻦ، ﻃﻮل ﺟﺎم، ﻧﻮع ﻛﺮك ﻛﺎﺳﻪ، ﻃﻮل ﻛﺮك ﻛﺎﺳﻪ و ﻟﻮﻟﻪ ﺟﺎم ﺻﻔﺎت اﻓﺘﺮاﻗﻲ ﻣﺤﺴﻮب ﻣﻲﺷﻮﻧﺪ. در ﺑﺮرﺳﻲ ﺳﺎﺧﺘﻤﺎن ﺗﺸﺮﻳﺤﻲ ﺻﻔﺎت وﺟﻮد ﻛﻼﻧﺸﻴﻢ ﻳﻚﻻﻳﻪ در ﺳﻄﺢ ﭘﺸﺘﻲ ﻳﺎ ﺷﻜﻤﻲ و ﻳﺎ ﻫﺮ دو ﺳﻄﺢ؛ وﺟﻮد ﭘﺎراﻧﺸﻴﻢ در ﻫﺮ دوﺳﻄﺢ و ﻳﺎ ﻓﻘﻂ ﻳﻜﻲ از ﺳﻄﻮح؛ وﺟﻮد ﻓﻴﺒﺮ در ﻫﺮ دو ﺳﻄﺢ، ﺳﻄﺢ ﭘﺸﺘﻲ و ﻳﺎ ﺳﻄﺢ ﺷﻜﻤﻲ و ﻳﺎﻓﻘﺪان آن؛ وﺟﻮد اﺳﻜﻠﺮاﻧﺸﻴﻢ ﺑﻴﻦ ﭼﻮب و آﺑﻜﺶ و ﻳﺎ ﻓﻘﺪان آن؛ وﺟﻮد ﭘﺎراﻧﺸﻴﻢ ﻧﺮدﺑﺎﻧﻲ در رﮔﺒﺮگ ﻣﺮﻛﺰي و ﺗﻌﺪاد ﺳﻠﻮل- ﻫﺎي روزﻧﻪ در واﺣﺪ ﺳﻄﺢ از ﺻﻔﺎت داراي ارزش اﻓﺘﺮاﻗﻲ ﺑﻴﻦ ﮔﻮﻧﻪﻫﺎ ﻫﺴﺘﻨﺪ.
    [Show full text]
  • Palynological Evolutionary Trends Within the Tribe Mentheae with Special Emphasis on Subtribe Menthinae (Nepetoideae: Lamiaceae)
    Plant Syst Evol (2008) 275:93–108 DOI 10.1007/s00606-008-0042-y ORIGINAL ARTICLE Palynological evolutionary trends within the tribe Mentheae with special emphasis on subtribe Menthinae (Nepetoideae: Lamiaceae) Hye-Kyoung Moon Æ Stefan Vinckier Æ Erik Smets Æ Suzy Huysmans Received: 13 December 2007 / Accepted: 28 March 2008 / Published online: 10 September 2008 Ó Springer-Verlag 2008 Abstract The pollen morphology of subtribe Menthinae Keywords Bireticulum Á Mentheae Á Menthinae Á sensu Harley et al. [In: The families and genera of vascular Nepetoideae Á Palynology Á Phylogeny Á plants VII. Flowering plantsÁdicotyledons: Lamiales (except Exine ornamentation Acanthaceae including Avicenniaceae). Springer, Berlin, pp 167–275, 2004] and two genera of uncertain subtribal affinities (Heterolamium and Melissa) are documented in Introduction order to complete our palynological overview of the tribe Mentheae. Menthinae pollen is small to medium in size The pollen morphology of Lamiaceae has proven to be (13–43 lm), oblate to prolate in shape and mostly hexacol- systematically valuable since Erdtman (1945) used the pate (sometimes pentacolpate). Perforate, microreticulate or number of nuclei and the aperture number to divide the bireticulate exine ornamentation types were observed. The family into two subfamilies (i.e. Lamioideae: bi-nucleate exine ornamentation of Menthinae is systematically highly and tricolpate pollen, Nepetoideae: tri-nucleate and hexa- informative particularly at generic level. The exine stratifi- colpate pollen). While the
    [Show full text]
  • Habitats Change in Ceylanpinar State's Farm and the Dangerous Classes of Plants
    International Journal of Science and Research (IJSR) ISSN: 2319-7064 Index Copernicus Value (2016): 79.57 | Impact Factor (2017): 7.296 Habıtats Change ın Ceylanpınar State’s Farm and the Dangerous Classes of Plants (Şanlıurfa-Turkey) Mustafa ASLAN2 1, 2Department of Biology, Faculty of Education, Harran University, 63510 Osmanbey Campus, Şanlıurfa-Turkey. Abstract: The natural flora of Ceylanpınar State Farm was studied in the years 2014 and 2015 before cultivation. During the floristic surveys, 146 species of 99 genera belonging to 18 families were recorded. 28 plant species among them are in threatened categories. Of these plants 13 are endemic and 15 are rare plants. However, the region of interest in this study has not been studied extensively and the habitats of this region have been constantly changing. In the natural flora of the region, rare and endemic plant species have been defined and their threat categories and the factors threatening these species have been determined. Keywords: Ceylanpınar State Farm, natural flora of Ceylanpınar,, rare and endemic plant species, threat categories of plants species 1. Introduction highest number of species are Astragalus 7, Bromus 5, Avena 5, Onobrychis 3, Trifolium 3 and Salvia 3 (Adigüzel Accelerated industrialization and rapid increase in human et.al 2002). population have had adverse effects on natural environments. As a result, human beings as well as the other livings have been affected. Nowadays, the environmental issues are no longer confined in national borders but regarded in international platforms. The countries of the world have assembled in many occasions to discuss the issues on environmental problems.
    [Show full text]
  • Biotechnology: an Indian Journal
    BioTechnology: An Indian Journal Research | Vol 12 Iss 8 Antimicrobial Activity of Ziziphora clinopodioides Essential Oil and Extract on Salmonella enterica, Staphylococcus aureus and Saccharomyces cerevisiae in Low Fat Mayonnaise Sinaeyan S and Sani AM* Young Researchers and Elite Club, Quchan Branch, Islamic Azad University, Quchan, Iran *Corresponding author: Sani AM, Young Researchers and Elite Club, Quchan Branch, Islamic Azad University, Quchan, Iran, E-mail: [email protected] Received: June 27, 2016; Accepted: July 05, 2016; Published: July 08, 2016 Abstract Ziziphora clinopodioides is a plant from Lamiaceae family. In this research the antimicrobial activity of Z. clinopodioides essential oil (at 0.05%) and extract (at 0.4%) were studied on Salmonella enterica, Staphylococcus aureus and Saccharomyces cerevisiae in mayonnaise during storage time at 4°C. Results showed that the antimicrobial effect of Z. clinopodioides essential oil was higher than the extract. This effect increased with increasing the concentration rate. The Z. clinopodioides essential oil was able to inhibit both gram-positive and gram negative bacteria and also the yeast. At the concentrations of Z. clinopodioides essential oil and extract we tested in the current study, we observed no unfavorable effect on the sensory parameters in mayonnaise. Keywords: Mayonnaise; Ziziphora clinopodioides; Salmonella enterica; Staphylococcus aureus; Saccharomyces cerevisia Introduction Mayonnaise is an oil in water (o/w) emulsion [1]. It is traditionally prepared by fully mixing egg yolk, oil, vinegar and spices including mustard to maintain closely packed foam of oil droplets; it may also include salt, sugar or sweeteners, and other optional ingredients. The emulsion is formed by slowly blending oil with a pre-mix that consists of egg yolk, vinegar, and mustard [2].
    [Show full text]
  • Computer-Generated Keys to the Flora of Egypt. 8. the Lamiaceae A
    16 Egypt. J. Bot. Vol. 59, No.1, pp. 209 - 232 (2019) Computer-Generated Keys to the Flora of Egypt. 8. The Lamiaceae A. El-Gazzar(1)#, A. El-Ghamery(2), A.H. Khattab(3), B.S. El-Saeid(2), A.A. El-Kady(2) (1)Botany and Microbiology Department, Faculty of Science, El-Arish University, El- Arish, N. Sinai, Egypt; (2)Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt; (3)The Herbarium, Botany Department, Faculty of Science, Cairo University, Cairo, Egypt. ANUALLY-constructed keys to many groups of the Egyptian flora are in urgent Mneed of improvement and updating. To construct a conventional substitute of the key to representatives of the Lamiaceae, a data matrix was compiled to include 48 characters recorded for each of the 52 species (with three subspecies and one variety) belonging to 24 genera which represent this family in the flora of Egypt. The 48 characters were accurately defined to cover as much of the easily observable aspects of vegetative and floral variation in the plants as possible. The data matrix was analyzed using the key-generating package of programs DELTA. The analysis produced a conventional key with a detailed description of every species in terms of the 48 characters. The key is decidedly a marked improvement over its predecessors in that it is strictly comparative. Updating the nomenclature of the plants led to the first recording of the genusThymbra in the flora of Egypt. Keywords: Conventional key, DELTA, Egypt, Flora, Lamiaceae, Thymbra. Introduction verticillasters in acropetal succession where the number of flowers per bract axil varies from 1 to The Lamiaceae Lindl.
    [Show full text]
  • MATHEMATICAL MODELING and SIMULATION of SUPERCRITICAL CO2 EXTRACTION of ZIZIPHORA TENUIOR VOLATILES Mojtaba Sinaei NOBANDEGANI1
    Anadolu Üniversitesi Bilim ve Teknoloji Dergisi A- Uygulamalı Bilimler ve Mühendislik Anadolu University Journal of Science and Technology A- Applied Sciences and Engineering 2016 - Volume: 17 Number: 1 Page: 1 - 11 DOI: 10.18038/btda.67720 Received:04 February 2015 Accepted: 06 September 2015 MATHEMATICAL MODELING AND SIMULATION OF SUPERCRITICAL CO2 EXTRACTION OF ZIZIPHORA TENUIOR VOLATILES Mojtaba Sinaei NOBANDEGANI1, Tayebeh DARBANDI2, Bizhan HONARVAR3, Mohammad Mohsen SARAFRAZ4 1Department of Chemical Engineering, International Campus, University of Sistan & Baluchestan, Chabahar, Iran 2Department of Chemical Engineering, Shahrood Branch, Islamic Azad University, Shahrood, Iran 3Department of Chemical Engineering, University of Fars Science and Research, Islamic Azad University, Iran 4School of Chemical, Gas and Petroleum Engineering, Semnan University, Semnan, Iran ABSTRACT Ziziphora Tenuior is an edible medicinal plant which belongs to Labiatae family. It is often used as a treatment for some diseases such as edema, insomnia, and hypertension in Turkey, Iran and China. The main components of the Ziziphora Tenuior essential oil are p-mentha-3-en-8-ol and pulegone. In this study, the extractions of Ziziphora essential oil has been described by a two-dimensional mathematical model, and the effects of some extraction parameter variations on the extraction yield have been examined. Amongst the said parameters were fluid flow rate, extractor diameter and length and mean particle size. Keywords: Carbon dioxide, Extraction, Supercritical Fluid, Modeling, Ziziphora Tenuior 1. INTRODUCTION The Labiatae family is a large family of herbs with some members such as peppermint, spearmint, rosemary and ziziphora. This family has aromatic species, which are used as culinary and therapeutic [1, 2]. Ziziphora is an herbaceous, flocculent, slender, edible, medicinal and erect plant with a height of 5-15 cm [3-5].
    [Show full text]
  • Beliefs of Herbal Therapies of Ilam
    © 2018 Journal of Pharmacy & Pharmacognosy Research, 6 (4), 299-317, 2018 ISSN 0719-4250 http://jppres.com/jppres Original Article | Artículo Original Beliefs of herbal therapies of the community of the Ilam city of Ilam province, Iran [Creencias de las terapias herbales de la comunidad de la ciudad de Ilam de la provincia de Ilam, Irán] Amir S. Mozaffari Nejad1,*, Mahmoud Bahmani2, Naseer A. Shah3, Sayed A. Shah4, Mahmoud Rafieian-Kopaei5,* 1Nutrition Health Research Center, Student Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. 2Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran. 3Deartment of Biosciences, COMSATS Institute of Information Technology, Islamabad, Pakistan. 4Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University Islamabad, 45320, Pakistan. 5Medical Plants Research Center, Basic Health Research Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran. *E-mail: [email protected], [email protected] Abstract Resumen Context: Documenting folk uses of wild medicinal plants provide an Contexto: La documentación del uso de la gente de las plantas silvestres important baseline for the future novel drugs development and produce una base importante para el desarrollo futuro de nuevos pharmacological evaluation. medicamentos y su evaluación farmacológica. Aims: To document the traditional ethnomedicinal knowledge of Ilam Objetivos: Documentar el conocimiento tradicional etno-medicinal de la city of Ilam Province, Iran. ciudad de Ilam de la provincia de Ilam, Irán. Methods: Semi-structured interviews were carried out in the study area Métodos: Se realizaron entrevistas semiestructuradas en el área de estudio from 190 informants to document the ethnomedicinal knowledge during de 190 informantes para documentar el conocimiento etno-medicinal 2008-2014.
    [Show full text]
  • Chemical Variation of Ziziphora Clinopodioides Subsp. Rigida (Boiss) Rech
    Journal of Medicinal Plants and By-products (2021) 1: 75-80 Original Article Chemical Variation of Ziziphora clinopodioides subsp. rigida (Boiss) Rech. f. from the Central of Iran Mohammad Hossein Mirjalili1, Fatemeh Amiri2,3 and Seyed Mostafa Goldansaz2,3* 1Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran 2Mahan Golsay Company, Yazd, Iran 3Iranian Medicinal Plants Society, Tehran, Iran Article History: Received: 28 July 2020/Accepted in revised form: 15 September 2020 © 2012 Iranian Society of Medicinal Plants. All rights reserved. Abstract Because of the special situation of medicinal plants and their natural product on human health, researchers are interested to discover useful constituents. The present research aims to discover the level of variation in oil of Ziziphora clinopodioides subsp. rigida (Boiss) Rech. f. at full flowering stage in Yazd province. All samples were approved in herbarium of Yazd University and dried out in shade and laboratory situation. Then 50 grams of each sample were powdered. In order to extract the oil of each community, the dried materials were subjected by hydro-distillation method (clavenger types). Resulted oils were dried by anhydrous sodium sulfate and kept in tightly closed vials at 4 °C before chemical analyses. The GC-FID and GC–MS analyses identified 29 compounds among which pulegone (18.1-51.4%), piperitenone (10.3- 23.3%), and 1,8-Cineole (8.6-15.6%) were the major component in all populations. It is indicated that the present result is similar to the previous study. In addition, Damgahan and Tezerjan had the highest amount of essential oil and pulegone in all studied populations, respectively.
    [Show full text]
  • Ethno Botanical Survey of Herbal Remedies Traditionally Used In
    Arom & at al ic in P l ic a n d Mashayekhan et al., Med Aromat Plants 2015, 4:3 t e s M Medicinal & Aromatic Plants DOI: 10.4172/2167-0412.1000192 ISSN: 2167-0412 ResearchResearch Article Article OpenOpen Access Access Ethno Botanical Survey of Herbal Remedies Traditionally Used in North Khorasan Province of Iran Mashayekhan A1,*, Pourmajidian MR2, Jalilvand H2, Gholami MR3 and Teimouri MS4 1Young Researchers and Elite Club, Golestan Branch, Islamic Azad University, Gorgan, Iran 2Sari Agriculture and Natural Resources, Faculty of Natural Resources, Department of Forestry, Sari, Iran 3Kowsar University of Bojnord, Bojnord, Iran 4Academic Center for Education, Culture and Research, Mashahd, Iran Abstract This study was carried out in north east of Iran to collect the information of traditionally used medicinal plants by the local communities. A total of 10 medicinal plants belonging to 6 families, have a traditional medicinal role in the treatment of severe cold, kidney disorder, hysteria, nervous affection, aroma, febrifuge, jaundice, rheumatism, gout, urinary infection, asthma and fever. Analysis showed that Lamiaceae were the most dominant families of medicinal plants in Darkesh area (18%). The most used part of the plants was the leaves at 50% and Decoction was the most popular form of treatment used. Some of the medicinal plants (Kakoti, Zereshk Kuhi, Shir Khesht) discussed in this article have new implications presented for the first time in northeastern Iran. Therefore, these findings are important for the management of aroma, febrifuge and jaundice. Local communities, especially, forest dweller heavily use these traditionally available medicinal plants for health and believe that these are easily available, less expensive and have no side effects.
    [Show full text]
  • Flora of Wadi Al-Quff Protected Area, Hebron Governorate, Palestine
    Al Sheikh & Mahassneh 47 Flora of Wadi Al-Quff Protected Area, Hebron Governorate, Palestine Banan Al Sheikhª and Mohamed Mahassnehb ª National Agricultural Research Council, Jenin, Palestine and Palestine Museum of Natural History, Bethlehem University, Bethlehem, Palestine [email protected] b Environmental Quality Authority, Ramallah, Palestine ABSTRACT We present data based on a preliminary survey of the flora of the Wadi Al-Quff Protected Area (WAQPA), Hebron Governorate in the Occupied Palestinian Territories (OPT). A total of 231 identified species plus ferns were reported through quadrates 20x20m for trees and shrubs and 1x1m for herbaceous plants. Dominant trees and shrubs include Pinus halapensis, Rhamnus palaestina, Quercus calliprinos, Sarcopotrium spinosum, and Cistus creticus. Crataegus aronia, Origanum syriacum, Pistacia lentiscus, and Styrax officinalis are less common in the reserve and need special attention. Twenty three species are rare at OPT level but were found at WAQPA and thus require protection. Key words: Wadi Al-Quff, Hebron, Protected areas, flora. INTRODUCTION Palestine enjoys a rich flora in spite of its small area due to its geographical position as a meeting point between Asia and Africa, where three phytogeographical regions intersect: Mediterranean, Irano-Turanian and Saharo-Arabian. There have been some studies of the flora of our region (Post, 1933; Zohary, 1966, 1972; Feinburn-Dothan, 1978; Zohary & Feinbrun-Dothan, 1986; Danin, 1992; Fragman et al., 1999; Al-Shaikh et al., 2000). An estimate of 2655 plant species occur in historic Palestine, while 1591 species were recorded from the West Bank (Al Sheikh et al., 2000). More recently 21 additional species were added to the flora of the West Bank (Al-Shaikh, personal communication).
    [Show full text]
  • Floristic Analysis and Biogeography of Tubiflorae in Egypt
    Volume 51(1):65-80, 2007 Acta Biológica Szegediensis http://www.sci.u-szeged.hu/ABS ARTICLE Floristic analysis and biogeography of Tubiflorae in Egypt Nahed El-Husseini1, Monier M Abd El-Ghani1*, Salah I El-Naggar2 The Herbarium, Faculty of Science, Cairo University, Giza, Egypt, 2 Botany Department, Faculty of Science, Assiut University, Egypt ABSTRACT The species distribution and biogeography of the Egyptian Tubiflorae were exam­ KEY WORDS ined in detail. We found 284 species of vascular plants belonging to 96 genera and 12 families, geographical distribution making the Egyptian Tubiflorae richer in species than that of other arid region floras: Libya and gamma diversity Saudi Arabia. The most species rich families were Scrophulariaceae, Boraginaceae, Labiatae, desert ecosystems Convolvulaceae and Solanaceae, constituting more than 85% of the total species in the order. flora The generic spectrum dominated by a suite of species-rich genera (Convolvulus, Heliotropium, endemism Veronica, Solanum, Salvia, Cuscuta, Echium, Ipomoea and Orobanche). Therophytes were the chorology most dominant life forms among the families, followed by chamaephytes and hemicryptophytes. Boraginaceae and Scrophulariaceae had the highest share of annuals. Remarkable distribution patterns of the life forms in the seven studied biogeographic zones were noticed. Trees were dominant in the Mediterranean zone, while shrubs, perennial herbs and therophytes were dominant in the Sinai. Altogether 8 endemic species and 14 near-endemics were included in the Tubiflorae of Egypt; mostly from southern Sinai. We found that Labiatae and Scrophulariaceae were the families with higher concentration of endemics. Notably, Teucrium was among the genera of the Mediterranean Africa with highest endemism.
    [Show full text]
  • The Effect of Different Levels of Altitutes on Composition and Content of Essential Oils of Ziziphora Clinopodioides in Southern of Turkey
    Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi (UTYHBD), 2015, 1(1): 1 - 6 Research article International Journal of Agriculture and Wildlife Science (IJAWS) The Effect of Different Levels of Altitutes on Composition and Content of Essential Oils of Ziziphora clinopodioides in Southern of Turkey Hasan Maral1 Hassan Taghikhani2 Alpaslan Kaya3 Saliha Kırıcı2 1Karamanoğlu Mehmetbey University, Ermenek Vocational School, Karaman, Turkey 2Çukurova University, Faculty of Agriculture, Department of Field Crops, Adana, Turkey 3 Mustafa Kemal University, Faculty of Agriculture, Department of Field Crops, Hatay, Turkey Received: 31.03.2015 Accepted: 04.06.2015 Key words: Abstract. The genus Ziziphora L. belongs to the Lamiaceae family and consists of Ziziphora clinopodioides, Ermenek, four species (Ziziphora clinopodioides, Ziziphora capitata, Ziziphora persica and essential oil Ziziphora tenuior). Z. clinopodioides is an edible medicinal plant that its leaves, flowers and stems are frequently used as wild vegetable or additive in foods to offer aroma and flavor. The plant known locally as ‘Kır nanesi’ is used in the preparation of an aromatic tea for gastrointestinal disorders and as a carminative, *Corresponding author antiseptic and wound healing material in Turkey. In this study, essential oil content e-mail: [email protected] and compositions of three Ziziphora clinopodioides plant were collected from Ermenek (Southern part of Turkey). The altitudes of collected places of plants were varied between 908 m and 1286 m. from sea level. The essential oil content of Z. clinopodioides plants was hydro-distilled by Clevenger apparatus, and they were determined between 0.12 and 0.50%. The highest essential oil content (0.50%) was obtained from 908 m.
    [Show full text]