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Lab Manual Biology Strictly in accordance with the latest core syllabus Saraswati LAB MANUAL BIOLOGY For Class XI By Rajesh Kumar M.Sc., B.Ed. New Saraswati House (India) Pvt. Ltd. Second Floor, MGM Tower, 19, Ansari Road, Daryaganj, New Delhi-110002 Ph: 43556600 Fax: 43556688 E-mail: [email protected] Website: www.saraswatihouse.com Branches Ahmedabad: (079) 22160722 Bengaluru: (080) 26619880 Chennai: (044) 24346531 Dehradun: 09837452852 Guwahati (0361) 2457198 Hyderabad: (040) 42615566 Jaipur: (0141) 4006022 Jalandhar: (0181) 4642600, 4643600 Kochi: (0484) 4033369 Kolkata (033) 40042314 Lucknow: (0522) 4062517 Mumbai: (022) 28737050, 28737090 Patna: (0612) 2570403 Ranchi: (0651) 2244654 Reprint 2016 ISBN: 978-81-7335-558-5 SYLLABUS Evaluation Scheme Maximum Marks: 30 One Major Experiment 5 Marks One Minor Experiment 4 Marks Slide Preparation 5 Marks Spotting 7 Marks Practical Record + Viva Voce 4 Marks Project Record + Viva Voce 5 Marks Total 30 Marks A. List of Experiments 60 Periods B. Study/observation of the following (spotting) 1. Study and describe three locally available common 1. Study parts of a compound microscope. flowering plants, one from each of the families 2. Study of the specimens /slides/models and Solanaceae, Fabaceae and Liliaceae including identification with reasons—Bacteria, Oscillatoria, dissection and display of floral whorls, anther and Spirogyra, Rhizopus, Mushroom, Yeast, Liverwort, ovary to show number of chambers (floral fomulae and Moss, Fern, Pines, one monocotyledonous plant and floral diagrams). Types of root (tap and adventitious), one dicotyledonous plant and one lichen. stem (herbaceous and woody), leaf (arrangement, 3. Study of virtual specimens/slides/models and shapes, venation, simple and compound). identification with reasons—Amoeba, Hydra, Liver fluke, Ascaris, leech, earthworm, prawn, silkworm, 2. Preparation and study of T.S. of dicot and monocot honeybee, snail, starfish, shark, rohu, frog, lizard, roots and stems (primary). pigeon and rabbit. 3. Study of osmosis by potato osmometer. 4. Study of tissues and diversity in shapes and sizes of plant and animal cells (palisade cells, guard cells, 4. Study of plasmolysis in epidermal peels (e.g., Rhoeo parenchyma, collenchyma, sclerenchyma, xylem, leaves). phloem, squamous epithelium, muscle fibres and 5. Study of distribution of stomata in the upper and mammalian blood smear) through temporary/ lower surface of leaves. permanent slides. 5. Study of mitosis in onion root tip cells and animal 6. Comparative study of the rates of transpiration in cells (grasshopper) from permanent slides. the upper and lower surface of leaves. 6. Study of different modifications in root, stem and 7. Test for the presence of sugar, starch, proteins and leaves. fats. To detect them in suitable plant and animal 7. Study and identification of different types of materials. inflorescences (cymose and racemose). 8. Study of imbibition in seeds/raisins. 8. Separation of plant pigments through paper 9. Observation and comments of the experimental set- chromatography. up for showing: 9. To study the rate of respiration in flower buds/leaf (a) Anaerobic respiration tissue and germinating seeds. (b) Phototropism 10. To test the presence of urea in urine. (c) Effect of apical bud removal (d) Suction due to transpiration 11. To detect the presence of sugar in urine. 10. Study of human skeleton and different types of joints 12. To detect the presence of albumin in urine. with the help of virtual images/models only. 11. Study of external morphology of cockroach through 13. To detect the presence of bile salts in urine. models. (ii) LAB MANUAL BIOLOGY XI (2014) New.pdf 2 9/6/2014 4:57:514:59:44 PM S.No. Experiments Page No. Introduction ............................................................................................................................................................ 5 SECTION-A 1. Study and Description of Three Locally Available Common Flowering Plants ................................................. 10 2. Preparation and Study of T.S. of Dicot and Monocot Roots and Stems (Normal/Primary) ............................... 16 3. Osmosis by Potato Osmoscope/Osmometer ......................................................................................................... 24 4. Plasmolysis in Epidermal Peels ........................................................................................................................... 26 5. Distribution of Stomata in Leaf Surfaces ............................................................................................................ 29 6. Comparative Rates of Transpiration by Cobalt Chloride Method ...................................................................... 32 7. Test for Presence of Sugar, Starch, Protein and Fat in Suitable Plant and Animal Materials. ........................ 35 8. Separate Chlorophyll Pigments through Paper Chromatography ..................................................................... 41 9. Rate of Respiration in Flower Buds/Leaf Tissue Germinating Seeds ................................................................44 10. Presence of Urea in Urine Sample ...................................................................................................................... 45 11. Presence of Sugar in Urine Sample ..................................................................................................................... 47 12. Presence of Albumin (Protein) in Urine Sample ................................................................................................. 49 13. Presence of Bile Salts in Urine Sample ............................................................................................................... 51 SECTION-B (SPOTTING) 1. Study of Compound Microscope ........................................................................................................................... 54 2. Study of Plant Specimens/slides/models ............................................................................................................. 57 3. Study of Animal Specimens/slides/models .......................................................................................................... 66 4A. Study of Plant Tissues.......................................................................................................................................... 74 4B. Study of Animal Tissues ....................................................................................................................................... 81 5. Study of Mitosis Cell Division.............................................................................................................................. 86 6. Study of Modifications in Root, Stem and Leaves............................................................................................... 91 7. Study and Identification of Inflorescences (cymose and racemose) ................................................................. 103 8. Study of Imbibition in Seeds/Raisins................................................................................................................. 112 9. Anaerobic Respiration, Phototropism in Plants, Apical Bud Removal and Suction due to Transpiration.................................................................................... 114 10A. Human Skeleton/Bones ...................................................................................................................................... 119 10B. Types of Joints in Human Body ......................................................................................................................... 122 11. Study of External Morphology of Cockroach..................................................................................................... 126 (iii) LAB MANUAL BIOLOGY XI (2014) New.pdf 3 9/6/2014 4:57:514:59:44 PM SECTION C Investigatory Project Experiments 1. Transpiration and Absorption Ratio .................................................................................................................. 129 2. Qualitative Ash Analysis .................................................................................................................................... 131 3. Separation of Plastidial Pigments ..................................................................................................................... 134 3.1. Separation of Plastidial Pigments—By Capillary Analysis ...................................................................... 134 3.2. Separation of Plastidial Pigments—By Benzene ...................................................................................... 135 3.3. Separation of Yellow and Green Pigments ................................................................................................ 136 4. Heat Release During Respiration ...................................................................................................................... 137 5. Fruit Ripening .................................................................................................................................................... 140 6. Growth in Plants ................................................................................................................................................ 142 7. Smoke Density .................................................................................................................................................... 144 8. Hydrolytic
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