An Example of an Autotroph Is
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Prokaryotes (Domains Bacteria & Archaea)
2/4/15 Prokaryotes (Domains Bacteria & Archaea) KEY POINTS 1. Decomposers: recycle organic and inorganic molecules in environment; makes them available to other organisms. 2. Essential components of symbioses. 3. Encompasses the origins of metabolism and metabolic diversity. 4. Origin of photosynthesis and formation of atmospheric Oxygen Ceno- Meso- zoic zoic ANTIQUITY Humans Paleozoic Colonization of land Animals Origin of solar system and Earth • >3.5 BILLION years old. • Alone for 2 1 4 billion years Proterozoic Archaean Prokaryotes Billions of 2 years ago3 Multicellular eukaryotes Single-celled eukaryotes Atmospheric oxygen General characteristics 1. Small: compare to 10-100µm for 0.5-5µm eukaryotic cell; single-celled; may form colonies. 2. Lack membrane- enclosed organelles. 3. Cell wall present, but different from plant cell wall. 1 2/4/15 General characteristics 4. Occur everywhere, most numerous organisms. – More individuals in a handful of soil then there are people that have ever lived. – By far more individuals in our gut than eukaryotic cells that are actually us. General characteristics 5. Metabolic diversity established nutritional modes of eukaryotes. General characteristics 6. Important decomposers and recyclers 2 2/4/15 General characteristics 6. Important decomposers and recyclers • Form the basis of global nutrient cycles. General characteristics 7. Symbionts!!!!!!! • Parasites • Pathogenic organisms. • About 1/2 of all human diseases are caused by Bacteria General characteristics 7. Symbionts!!!!!!! • Parasites • Pathogenic organisms. • Extremely important in agriculture as well. Pierce’s disease is caused by Xylella fastidiosa, a Gamma Proteobacteria. It causes over $56 million in damage annually in California. That’s with $34 million spent to control it! = $90 million in California alone. -
Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas Sp
microorganisms Article Characterization of the Aerobic Anoxygenic Phototrophic Bacterium Sphingomonas sp. AAP5 Karel Kopejtka 1 , Yonghui Zeng 1,2, David Kaftan 1,3 , Vadim Selyanin 1, Zdenko Gardian 3,4 , Jürgen Tomasch 5,† , Ruben Sommaruga 6 and Michal Koblížek 1,* 1 Centre Algatech, Institute of Microbiology, Czech Academy of Sciences, 379 81 Tˇreboˇn,Czech Republic; [email protected] (K.K.); [email protected] (Y.Z.); [email protected] (D.K.); [email protected] (V.S.) 2 Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark 3 Faculty of Science, University of South Bohemia, 370 05 Ceskˇ é Budˇejovice,Czech Republic; [email protected] 4 Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 370 05 Ceskˇ é Budˇejovice,Czech Republic 5 Research Group Microbial Communication, Technical University of Braunschweig, 38106 Braunschweig, Germany; [email protected] 6 Laboratory of Aquatic Photobiology and Plankton Ecology, Department of Ecology, University of Innsbruck, 6020 Innsbruck, Austria; [email protected] * Correspondence: [email protected] † Present Address: Department of Molecular Bacteriology, Helmholtz-Centre for Infection Research, 38106 Braunschweig, Germany. Abstract: An aerobic, yellow-pigmented, bacteriochlorophyll a-producing strain, designated AAP5 Citation: Kopejtka, K.; Zeng, Y.; (=DSM 111157=CCUG 74776), was isolated from the alpine lake Gossenköllesee located in the Ty- Kaftan, D.; Selyanin, V.; Gardian, Z.; rolean Alps, Austria. Here, we report its description and polyphasic characterization. Phylogenetic Tomasch, J.; Sommaruga, R.; Koblížek, analysis of the 16S rRNA gene showed that strain AAP5 belongs to the bacterial genus Sphingomonas M. Characterization of the Aerobic and has the highest pairwise 16S rRNA gene sequence similarity with Sphingomonas glacialis (98.3%), Anoxygenic Phototrophic Bacterium Sphingomonas psychrolutea (96.8%), and Sphingomonas melonis (96.5%). -
A Study on the Phototrophic Microbial Mat Communities of Sulphur Mountain Thermal Springs and Their Association with the Endangered, Endemic Snail Physella Johnsoni
A Study on the Phototrophic Microbial Mat Communities of Sulphur Mountain Thermal Springs and their Association with the Endangered, Endemic Snail Physella johnsoni By Michael Bilyj A thesis submitted to the Faculty of Graduate Studies in partial fulfillment of the requirements for the degree of Master of Science Department of Microbiology Faculty of Science University of Manitoba Winnipeg, Manitoba October 2011 © Copyright 2011, Michael A. Bilyj 1 Abstract The seasonal population fluctuation of anoxygenic phototrophs and the diversity of cyanobacteria at the Sulphur Mountain thermal springs of Banff, Canada were investigated and compared to the drastic population changes of the endangered snail Physella johnsoni. A new species and two strains of Rhodomicrobium were taxonomically characterized in addition to new species of Rhodobacter and Erythromicrobium. Major mat-forming organisms included Thiothrix-like species, oxygenic phototrophs of genera Spirulina, Oscillatoria, and Phormidium and purple nonsulfur bacteria Rhodobacter, Rhodopseudomonas and Rhodomicrobium. Aerobic anoxygenic phototrophs comprised upwards of 9.6 x 104 CFU/cm2 of mat or 18.9% of total aerobic heterotrophic bacterial isolates at certain sites, while maximal purple nonsulfur and purple sulfur bacteria were quantified at 3.2 x 105 and 2.0 x 106 CFU/cm2 of mat, respectively. Photosynthetic activity measurements revealed incredibly productive carbon fixation rates averaging 40.5 mg C/cm2/24 h. A temporal mismatch was observed for mat area and prokaryote-based organics to P. johnsoni population flux in a ―tracking inertia‖ manner. 2 Acknowledgements It is difficult to express sufficient gratitude to my supervisor Dr. Vladimir Yurkov for his unfaltering patience, generosity and motivation throughout this entire degree. -
Worksheet Class 7Th ( Science ) Chapter 1St Nutrition in Plants
Worksheet Class 7th ( science ) Chapter 1st Nutrition in plants 1. Autotrophic nutrition 2. Heterotrophic Nutrition The mode of nutrition in which organisms obtain their food from others ( plants and animals ) is called heterotrophic nutrition. Heterotrophs :- Organisms that are not capable of synthesising their own food and depend on other organisms for their food requirements are called heterotrophs. They are also called consumers. Heterotrophic Nutrition in plants Heterotrophic nutrition in non-green plants are of three types- (i) Saprotrophic (ii) Parasitic (iii) Symbiotic (I) Saprotrophic nutrition The mode of nutrition in which organisms take in nutrients from dead and decaying matter is called saprotrophic nutrition. Saprotrophs or saprophytes Saprotrophs are the organisms that feed on dead and decaying matter. Example :- Fungi, mushrooms Saprophytes are also called cleaners of the environment. (II) Parasitic Nutrition The mode of nutrition in which an organism lives on or inside the body of other living organism (host) is called parasitic nutrition. Parasitic plants are of two types • Total parasites • Partial parasites Total parasites These plants cannot make their own food and derive all of it from the host plant. E.g.- cuscuta (amarbel) is total stem parasite and Rafflesia is total root parasite plant. Partial parasites They have green leaves, therefore can make their food for themselves. However, they get water and minerals from host plant. E.g.- mistletoe is a partial stem parasite and sandalwood is a partial root parasite. (III) Symbiotic Nutrition Symbionts:- Two organisms living in close physical contact with each other and providing mutual benefits are called symbionts. Symbiosis:- Condition of living together is called symbiosis. -
Aerobic Respiration
Life is based on redox • All energy generation in biological systems is due to redox (reduction-oxidation) reactions Aerobic Respiration: + - C6H12O6 + 6 H2O ==> 6 CO2 + 24 H +24 e oxidation electron donor (aka energy source) + - (O2+ 4H + 4e ==> 2H2O) x6 reduction electron acceptor --------------------------------------- C6H12O6 + 6 O2 ==> 6 CO2 + 6 H2O overall reaction (24 electrons) Types of bacterial metabolisms • While eukaryotes only reduce O2 and oxidize organic compounds, prokaryotes can use a variety of electron donors and acceptors, organic and inorganic. - • Aerobic respiration: e acceptor is O2 - • Anaerobic respiration: e acceptor is not O2 • Fermentation: e- donor and acceptor are organic molecules • Chemolithotrophy: e- donor and acceptor are inorganic molecules • Phototrophy: e- donor is light and e- acceptor is either organic or inorganic all microorganisms energy source? chemical light chemotroph phototroph carbon source? carbon source? organic organic CO CO compound 2 compound 2 chemoheterotroph chemoautotroph photoheterotroph photoautotroph e- acceptor? Nitrifying and sulfur- use H O to reduce CO ? oxidizing bacteria 2 2 green non-sulfur and O Other than O 2 2 purple non-sulfur bacteria anoxygenic oxygenic photosynthesis: photosynthesis: green sulfur and most bacteria Organic Inorganic cyanobacteria compound compound purple sulfur bacteria fermentative organism anaerobic respiration: nitrate, sulfate, Fe(III) Aerobic or anaerobic respiration Chemolithotrophy Important molecules Redox Electron Carrier: for example the -
Biophysical Aspects of Resource Acquisition and Competition in Algal Mixotrophs
vol. 178, no. 1 the american naturalist july 2011 Biophysical Aspects of Resource Acquisition and Competition in Algal Mixotrophs Ben A. Ward,* Stephanie Dutkiewicz, Andrew D. Barton, and Michael J. Follows Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 Submitted November 10, 2010; Accepted March 15, 2011; Electronically published June 6, 2011 polar waters, for example, mixotrophy provides dinofla- abstract: Mixotrophic organisms combine autotrophic and het- gellates with the flexibility to endure large environmental erotrophic nutrition and are abundant in both freshwater and marine environments. Recent observations indicate that mixotrophs consti- changes during tidal and seasonal cycles (Li et al. 2000; tute a large fraction of the biomass, bacterivory, and primary pro- Litchman 2007). However, in the low-seasonality sub- duction in oligotrophic environments. While mixotrophy allows tropical oceans, where such nonequilibrium dynamics are greater flexibility in terms of resource acquisition, any advantage presumably much less important, mixotrophy remains a must be traded off against an associated increase in metabolic costs, prevalent strategy. Zubkov and Tarran (2008) recently which appear to make mixotrophs uncompetitive relative to obligate found that photosynthetic protist species, which account autotrophs and heterotrophs. Using an idealized model of cell phys- iology and community competition, we identify one mechanism by for more than 80% of the total chlorophyll in regions of which mixotrophs can effectively outcompete specialists for nutrient the North Atlantic, were also responsible for 40%–95% of elements. At low resource concentrations, when the uptake of nu- the total bacterivory. Small mixotrophs have been shown trients is limited by diffusion toward the cell, the investment in cell to be of similar importance in coastal oligotrophic waters membrane transporters can be minimized. -
Nutritional and Reproductive Strategies in a Chemosymbiotic Bivalve Living in a Tropical Intertidal Seagrass Bed
Vol. 501: 113-126, 2014 MARINE ECOLOGY PROGRESS SERIES Published March 31 doi: 10.3354/mepsl0702 Mar Ecol Prog Ser OPEN ACCESS © ® Nutritional and reproductive strategies in a chemosymbiotic bivalve living in a tropical intertidal seagrass bed Matthijs van der Geest1*, Amadou Abderahmane Sail2, Sidi Ould Ely3, Reindert W. Nauta1, Jan. A. van Gils1, Theunis Piersma1,4 1NIOZ Royal Netherlands Institute for Sea Research, PO Box 59, 1790 AB Den Burg, Texel, The Netherlands 2Mauritanian Institute for Oceanographic Research and Fisheries (IMROP), BP 22, Nouadhibou, Mauritania 3Parc National du Banc d'Arguin, BP 5355, Nouakchott, Mauritania 4Chair in Global Flyway Ecology, Animal Ecology Group, Centre for Ecological and Evolutionary Studies (CEES), University of Groningen, PO Box 11103, 9700 CC Groningen, The Netherlands ABSTRACT: Sulphide-oxidizing endosymbiont-bearing bivalves often dominate the infauna of seagrass-covered sediments, where they control sulphide levels and contribute to carbon cycling by feeding on chemosynthetically fixed carbon and suspended particulate organic matter (SPOM). Previous studies from temperate habitats suggest that SPOM availability may regulate growth and reproduction, since SPOM may be of greater nutritional value than the material provided by bacterial endosymbionts. To examine if changes in diet correlate with body condition and repro ductive activity, we studied seasonal patterns in somatic and gonadal investment and gameto- genic development in relation to nutrition in the endosymbiont-bearing bivalveLoripes lucinalis in seagrass-covered intertidal flats at a tropical study site (Banc d'Arguin, Mauritania). Carbon stable isotope analysis revealed clear seasonal cycles in the relative heterotrophic contribution to the diet of Loripes, with mean monthly values ranging from 21% in March to 39% in September. -
Nitrogen and Phosphorus Limitation of Oceanic Microbial Growth During Spring in the Gulf of Aqaba
Vol. 56: 227–239, 2009 AQUATIC MICROBIAL ECOLOGY Printed September 2009 doi: 10.3354/ame01357 Aquat Microb Ecol Published online August 27, 2009 Contribution to AME Special 2 ‘Progress and perspectives in aquatic primary productivity’ OPEN ACCESS Nitrogen and phosphorus limitation of oceanic microbial growth during spring in the Gulf of Aqaba David J. Suggett1,*, Noga Stambler2, Ondrej Prá$il3, Zbigniew Kolber4, Antonietta Quigg5, Evaristo Vázquez-Domínguez6, Tamar Zohary7, Tom Berman7, David Iluz8, Orly Levitan2, Tracy Lawson1, Efrat Meeder9,10, Boaz Lazar9,10, Edo Bar-Zeev2, Hana Medova3, Ilana Berman-Frank8 1Department of Biological Sciences, University of Essex, Colchester CO4 3SQ, UK 2Department of Geography and Environment, Bar-Ilan University, Ramat-Gan 52900, Israel 3Photosynthesis Laboratory, Institute of Microbiology ASCR, Opatovicky mlyn, 379 81 Trˇ ebonˇ, Czech Republic 4Monterey Bay Aquarium Research Institute, 7700 Sandholdt Road, Moss Landing, California 95039, USA 5Department of Marine Biology and Oceanography, Texas A&M University, Galveston, Texas 77551, USA 6CSIC, Institut de Ciències del Mar, Passeig Marítim de la Barceloneta 39-43, 08003 Barcelona, Spain 7Kinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, PO Box 447, Migdal 14950, Israel 8The Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel 9The Interuniversity Institute for Marine Sciences Coral Beach, PO Box 469, 88103 Eilat, Israel 10Institute of Earth Sciences, The Hebrew University Edmond J. Safra Campus, Jerusalem 91904, Israel ABSTRACT: Bioassay experiments were performed to identify how growth of key groups within the mi- crobial community was simultaneously limited by nutrient (nitrogen and phosphorus) availability during spring in the Gulf of Aqaba’s oceanic waters. -
IB HL Biology: Ecology Review Fall 2017 Populations 1. Define the Following Terms Associated with Population Ecology; Population and Carrying Capacity
IB HL Biology: Ecology Review Fall 2017 Populations 1. Define the following terms associated with population ecology; population and carrying capacity. 2. What processes contribute to changes in population size? 3. What are some factors which can increase the carrying capacity of a population? Decrease? 4. What is quadrat sampling? When would it be used? Communities 5. Define the following terms; community, autotroph, heterotroph, producer, primary consumer, secondary consumer, detritivore and saprotroph. 6. What is the initial energy source for all communities? 7. Be able to read food webs and determine the trophic level of different species. 8. Choose 2 regions below and determine the Simpson’s Diversity Index value for these regions. Which region is more diverse? A. An area of the Black Forest in Germany contains 134 pitch pines, 24 douglas firs, and 53 red pines. B. A meadow contains 1532 chestnut oaks, 342 black cherry trees, 12 white ash trees, and 1022 yellow birches. C. You school science classroom contains 12 beetles, 34 termites, 84 ants, 93 fleas, and 1 butterfly. D. An African park contains 15 lions, 94 giraffes, 1000 wildebeests, 50 elephants, and 5 hyenas. Choose more areas if you need more practice. 9. What is a keystone species? 10. Distinguish between primary and secondary succession. Ecosystems 11. What is an ecosystem? 12. Explain the 10% rule of energy transfer. How is the energy lost between trophic levels? 13. Review the Carbon Cycle. What are the main sources of carbon dioxide on earth? 14. Review the Nitrogen Cycle. 15. Distinguish between Gross Primary Productivity and Net Primary Productivity. -
Chapter 11 – PROKARYOTES: Survey of the Bacteria & Archaea
Chapter 11 – PROKARYOTES: Survey of the Bacteria & Archaea 1. The Bacteria 2. The Archaea Important Metabolic Terms Oxygen tolerance/usage: aerobic – requires or can use oxygen (O2) anaerobic – does not require or cannot tolerate O2 Energy usage: autotroph – uses CO2 as a carbon source • photoautotroph – uses light as an energy source • chemoautotroph – gets energy from inorganic mol. heterotroph – requires an organic carbon source • chemoheterotroph – gets energy & carbon from organic molecules …more Important Terms Facultative vs Obligate: facultative – “able to, but not requiring” e.g. • facultative anaerobes – can survive w/ or w/o O2 obligate – “absolutely requires” e.g. • obligate anaerobes – cannot tolerate O2 • obligate intracellular parasite – can only survive within a host cell The 2 Prokaryotic Domains Overview of the Bacterial Domain We will look at examples from several bacterial phyla grouped largely based on rRNA (ribotyping): Gram+ bacteria • Firmicutes (low G+C), Actinobacteria (high G+C) Proteobacteria (Gram- heterotrophs mainly) Gram- nonproteobacteria (photoautotrophs) Chlamydiae (no peptidoglycan in cell walls) Spirochaetes (coiled due to axial filaments) Bacteroides (mostly anaerobic) 1. The Gram+ Bacteria Gram+ Bacteria The Gram+ bacteria are found in 2 different phyla: Firmicutes • low G+C content (usually less than 50%) • many common pathogens Actinobacteria • high G+C content (greater than 50%) • characterized by branching filaments Firmicutes Characteristics associated with this phylum: • low G+C Gram+ bacteria -
Fermentation and Anaerobic Decomposition in a Hot Spring
Fermentation and anaerobic decomposition in a hot spring microbial mat by Karen Leigh Anderson A thesis submitted in partial fulfillment of requirements for the degree of Master of Science in Microbiology Montana State University © Copyright by Karen Leigh Anderson (1984) Abstract: Fermentation was investigated in a low sulfate hot spring microbial mat (Octopus Spring) according to current models on anaerobic decomposition. The mat was studied to determine what fermentation products accumulated, where in the mat they accumulated, and what factors affected their accumulation. Mat samples were incubated under dark anaerobic conditions to measure accumulation of fermentation products. Acetate and propionate (ca. 3:1) were the major products to accumulate in a 55°,C mat. Other products accumulated to a much lesser extent. Incubation of mat samples of varying thickness showed that fermentation occurred in the top 4mm of the mat. This has interesting implications for fermentative organisms in the mat due to the diurnal changes in mat oxygen concentrations. Fermentation measured in mat samples collected at various temperatures (50°,-70°C) showed acetate and propionate to be the major accumulation products. According to the interspecies hydrogen transfer model, the hydrogen concentration in a system affects the types of fermentation products produced. At a 65° C site, with natural high hydrogen levels, and at a 55°C site, with active methanogenesis, fermentation product accumulation was compared. There was a greater ratio of reduced fermentation products to acetate, with the exception of propionate, at 65°C. Ethanol accumulated at the 65°C site, as did lactate, though to a lesser extent. -
Hetrotrophic-Nutrition-O-Level.Pdf
Heterotrophic nutrition Heterotrophic organisms are organisms that feed on complex ready-made organic food. They use it as source of: - (i) energy for their vital activities, (ii) building materials, that is specific atoms and molecules for cell maintenance and repair and growth, (iii) vitamins (co-enzymes) that cannot be synthesised in organism but which are vital specific cellular processes. The main forms of heterotrophic nutrition include (i) holozoic, (ii) saprotrophic (or saprophytic) e.g. mould, mushroom (iii) mutualistic (iv) parasitic, although some overlap between groups may occur. Holozoic nutrition It is a type of heterotrophic nutrition involves the following processes (i) Ingestion: is taking in of complex organic food(solid or liquid). (ii) Digestion: is the breakdown of large complex insoluble organic molecules into small, simple soluble diffusible molecules. This is achieved by mechanical break down and enzymatic hydrolysis. Digestion may be either extra or intra cellular. (iii)Absorption: is the uptake of the soluble molecules from the digestion region, across a membrane and into the body tissue proper. The food may pass into the blood stream to be transported to appropriate regions within the body of the organism. (iv) Assimilation is the utilisation of the absorbed molecules by the body to provide either energy or materials to be incorporated into the body. (v) Egestion is the elimination from the body of undigested waste food materials. Animals which feed one plants are called herbivores, those that feed on other animals carnivores, and those that eat a mixed diet of animal and vegetable matter are termed omnivores. If they take in food in form of small particles the animals are microphagous feeders, for example earthworms, whereas if the food is ingested in liquid form they are, classed as fluid feeders, such as aphids and mosquitoes.