animals Article Environmental DNA Metabarcoding as a Means of Estimating Species Diversity in an Urban Aquatic Ecosystem Heather J. Webster 1, Arsalan Emami-Khoyi 1, Jacobus C. van Dyk 2, Peter R. Teske 1 and Bettine Jansen van Vuuren 1,* 1 Centre for Ecological Genomics and Wildlife Conservation, Department of Zoology, University of Johannesburg, Auckland Park, Gauteng 2006, South Africa;
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[email protected] Received: 13 October 2020; Accepted: 5 November 2020; Published: 7 November 2020 Simple Summary: Cities are the fastest developing ecosystems on the planet. The rapid expansion of urban areas is typically seen as a threat to global biodiversity, yet the role of cities in protecting species that may be rare in the wild remains poorly explored. Here, we report the use of environmental DNA (eDNA) to document the species present in one of the largest urban green spaces in Johannesburg, South Africa. We document a surprisingly large number of taxonomic groups, including some rare and threatened species. Our results support the notion that urban green spaces can provide refuge to a large number of species, and the species inventory provides critical information that can be used by city parks managers to conserve green spaces. Abstract: Adaptation to environments that are changing as a result of human activities is critical to species’ survival. A large number of species are adapting to, and even thriving in, urban green spaces, but this diversity remains largely undocumented.