Turkish Journal of Zoology Turk J Zool (2015) 39: 185-187 http://journals.tubitak.gov.tr/zoology/ © TÜBİTAK Short Communication doi:10.3906/zoo-1312-23

Copidosoma boucheanum Ratzeburg (Hym.: ): new record from Iran

1, 2 Hossein LOTFALIZADEH *, Mehri BAB-MORAD 1 Department of Plant Protection, East Azerbaijan Research Center for Agriculture and Natural Resources, Tabriz, Iran 2 Department of Plant Protection, Research Institute of Forests and Rangelands, Tehran, Iran

Received: 12.12.2013 Accepted: 17.03.2014 Published Online: 02.01.2015 Printed: 30.01.2015

Abstract: The encyrtid species Copidosoma boucheanum Ratzeburg (Hym.: Chalcidoidea, Encyrtidae) was identified and is reported for the first time from Iran. These specimens were reared from leafroller larvae (Lep.: ) on Populus sp. as a polyembryonic endoparasitoid. Copidosoma species of Iran are listed with their distribution.

Key words: Tortricidae, Copidosoma, biological control, distribution, new record

The chalcidoid family Encyrtidae (: were dropped into 75% ethanol for further examination. Chalcidoidea), with many successful introductions, is one Preparation of specimens was conducted following Noyes of the most important biological control agents of (1982). Specific identification of specimens was done pests. The genus Copidosoma, with 192 known species according to reliable keys and descriptions (Guerrieri and (Noyes, 2013), is a large genus of this family. Species of Noyes, 2005; Japoshvili et al., 2013). this genus are polyembryonic and therefore potential External morphology was illustrated using an Olympus biocontrol agents of pests (Guerrieri and SZH equipped with a Canon A720 digital camera. The Noyes, 2005). Guerrieri and Noyes (2005) revised the specimens were deposited in the insect collection of the European species (with 56 species). Sixty-five species were Department of Plant Protection, East Azerbaijan Research reported from Europe by Noyes (2013), who presented Center for Agriculture and Natural Resources, Tabriz, their diagnosis, hosts, and taxonomic status. Japoshvili et Iran. General data regarding geographical distribution and al. (2013) reviewed the Copidosoma of Norway, reporting biology, as well as brief taxonomic comments, are given for 16 species. The molecular phylogeny of this genus’ species this species. associated with Noctuidae was studied by Zhang et al. Examination of specimens showed that this species (2008). is from the genus Copidosoma Ratzeburg (Hymenoptera: The Encyrtidae fauna of Iran was recently studied Chalcidoidea, Encyrtidae), and it was identified as (Fallahzadeh and Japoshvili, 2010; Lotfalizadeh, 2010a, Copidosoma boucheanum Ratzeburg. Based on the 2010b) and includes 132 species. Only 3 species of available literature, this species has not previously been Copidosoma have been reported previously from reported from Iran. From each parasitized larvae, 50–60 Iran (Table): (Ashmead), C. female emerged. pistacinellae Hoffer, and C. varicorne (Nees) (Fallahzadeh Copidosoma boucheanum Ratzeburg, 1844 (Figures and Japoshvili, 2013); however, we are confident that this 1A–1C) number will grow considerably in the future. Materials examined: Iran, Alborz Province, Sirachal, The second author collected some torticid larvae Research Station, 23.vi.1991, ex Tortricidae on Populus sp., (Lep.: Tortricidae) from under the bark of Populus sp. in M. Bab-Morad leg., 20 ♀♀ and 3 ♂♂. spring. Some of these larvae were parasitized. All of the Diagnosis. All of the features of the specimens that materials were subsequently transferred to the entomology we collected in Iran match well with those reported by laboratory with natural conditions similar to those of the Guerrieri and Noyes (2005), except for the following collecting area and monitored each day. The emerged characters: 1) studied specimens with 3–4 mm length are parasitoids were carefully collected using an aspirator and slightly larger than specimens examined by Guerrieri and * Correspondence: [email protected] 185 LOTFALIZADEH and BAB-MORAD / Turk J Zool

Table. List of Copidosoma species reported from Iran and their distribution.

Species Distribution in Iran Reference Copidosoma boucheanum Ratzeburg, 1844 Alborz Province (Sirachal) New record Copidosoma floridanum (Ashmead 1900) East Azerbaijan Province (Marand) Lotfalizadeh (2010b) Copidosoma pistacinellae Hoffer, 1970 Iran* Hoffer (1970), Trjapitzin (1989) Copidosoma varicorne (Nees 1834) Azerbaijan, Markazi, and Tehran provinces Chodjai (1968), Sabzevari (1968)

*: This species was originally described from Iran without exact type locality.

Figure 1. Copidosoma boucheanum: A) female in lateral view; Figure 2. Parasitized larvae of Tortricidae: A) including B) forewing; C) head and antennae in lateral view. parasitoids; B) after emergence of parasitoids.

Noyes (2005); 2) fore coxae yellow, while Guerrieri and Geographical distribution. This is the first record of C. Noyes (2005) mentioned that they varied from completely boucheanum in Iran, but it is known from Europe, Central yellow to completely brown with a metallic green sheen; Asia, and the Middle East (Noyes, 2013). 3) exerted part of ovipositor varying from 1 to 1.1 times Hosts. These specimens were reared from leafroller as long as midtibia (Figure 1A), whereas it was mentioned larvae (Lep.: Tortricidae) on Populus sp. as a polyembryonic as 0.8–1.1 times as long by Guerrieri and Noyes (2005); 4) endoparasitoid (Figures 2A and 2B). antenna with scape long and slender, about 9 times as long Copidosoma boucheanum is a of different as broad, F1 about 6.6 times as long as broad and about 2 microlepidopterous families (Gelechiidae, Lymantriidae, times as long as pedicel (Figure 1C), while it was described Tortricidae, and Yponomeutidae) (Guerrieri and Noyes, as 6.4 times as long as broad by Guerrieri and Noyes (2005). 2005).

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References

Fallahzadeh M, Japoshvili G (2010). Checklist of Iranian Encyrtids Lotfalizadeh H (2010b). Some new data and corrections on Iranian (Hymenoptera: Chalcidoidea) with descriptions of new encyrtid wasp (Hymenoptera: Chalcidoidea, Encyrtidae) species. J Insect Sci 10: 1–24. fauna. Biharean Biologist 4: 173–178. Fallahzadeh M, Japoshvili G (2013). Corrections to the list of Noyes JS (1982). Collecting and preserving chalcid wasps Encyrtidae (Hymenoptera: Chalcidoidea) from Iran. J Entomol (Hymenoptera: Chalcidoidea). J Nat Hist 16: 315–334. Res Soc 15: 117–121. Noyes JS (2013). Universal Chalcidoidea database. Available Guerrieri E, Noyes J (2005). Revision of the European species from: www.nhm.ac.uk/entomology/chalcidoids/index.html of Copidosoma Ratzeburg (Hymenoptera: Encyrtidae), [accessed 1 September 2013]. parasitoids of (Lepidoptera). System Entomol 30: Sabzevari A (1968). Lepidopterous pest on apricot. In: Proceedings of 97–174. the First Iranian Plant Protection Congress, 13–18 September Hoffer A (1970). Bemerkungen zu drei wenig bekannten Arten 1968; Karaj, Tehran University, Iran. pp. 63–80. der famile Encyrtidae (Hymenoptera: Chalcidoidea) und Trjapitzin VA (1989). Parasitic Hymenoptera of the Fam. Encyrtidae Beschreibungen der weiteren, für die Wissenschaft neuen taxa. of Palaearctics. Opredeliteli po Faune SSSR. Leningrad, USSR: Stud Entomol Forestalia 1: 191–193 (in German). Zoologicheskim Institutom Akademii Nauk SSR. Japoshvili G, Hansen LO, Guerrieri E (2013). The Norwegian species Zhang YZ, Yu F, Zhu CD (2008). A preliminary phylogenetic study of of Copidosoma Ratzeburg (Hymenoptera: Chalcidoidea: Copidosoma spp. (Hymenoptera: Encyrtidae) associated with Encyrtidae). Zootaxa 3619: 145–153. Noctuidae (Lepidoptera) based on 28S rDNA D2 sequence. Lotfalizadeh H (2010a). The genus Metaphycus Mercet, 1917 (Hym.: Acta Entomol Sinica 51: 992–996. Encyrtidae) of the Iranian fauna with description of a new species. North-Western J Zool 6: 255–261.

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