Studies in the Genus Pleospora. Vii

March, 1952] WEHMEYER-PLEOSPORA 237

RCSSELL, R. S., AND R. P. MARTIN. 1949. Use of radio SHAHMAN, B. C. 1945. Leaf and bud initiation in the active phosphorus in plant nutritional studies. Nature Gramineae. Bot. Gaz. 106: 269-289. 163: 71-72. SMITH, G. F., AND H. KERSTEN. 1941. Root modifications SAX, K. 1940. An analysis of X-ray induced chromosome induced in Vieia [aba by irradiating dry seeds with aberrations in Tradescantia. Genetics 25: 4.1-68. soft X-rays. Plant Physiol. 16: 159-170.

STUDIES IN THE GENUS PLEOSPORA. VII

Lewis E. Wehmeyer

IN A PREVIOUS PAPER (Wehmeyer, 1952b), it was definitely inequilateral or curved. In P. alismatis, pointed out that the two types of septation char with similar spores, the nine-septate condition is acteristic of the leptosphaeroid and vulgaris series fixed. In P. rubicunda the ends of the ascospores in the genus Pleospora tend to converge above the are still more broadly rounded and the spores may five-septate level, and that very often both types of become eleven-septate, by the formation of secon septation appear in the same spore. In that paper, dary vulgaris septa in four of the central cells. the species with spores showing an asymmetric sep In P. morauica and P. saponariae, this type of tation were arbitrarily taken as representing the septation is well illustrated, for there is great varia leptosphaeroid series and discussed as such. bility in the septation of spores of varying maturity The present paper is to deal with those species showing this progression. The secondary vulgaris which show a similar but symmetric septation in type of septation may occur in any of the four cen the ascospore, and which present a more or less tral cells of the spores of these species, but this is parallel group. often delayed, or these septa are fainter than the The species of Pleospora with five-septate spores transverse septa first laid down. P. morauica has of the vulgaris type have already been considered a spore form similar to P. abscondita and differs (Wehmeyer, 1952a). There are some five recog from that species chiefly in the more symmetric nizable species (known to the writer) which rep mode of laying down the septa in the spore. P. resent the seven-septate condition, which arises by saponariae has larger spores approaching those of the insertion of a secondary transverse septum in P. rubicunda in form. each of the end cells of a five-septate spore. All of In table 1, data are given concerning host, and these species differ from P. trevoicola, previously spore, ascus and perithecial measurements of the discussed (Wehmeyer, 1952b), in the more con individual collections discussed. It is interesting to stantly symmetric spores. note that nearly all these species, except P. henning. Three of these species occur on monocotyledons. siana and P. saponariae, which are distinctive in P. punctata, on Scirpus, has small perithecia on other respects, have spores within a very similar limited gray spots, and ascospores which are some size range, i.e., 25--40 fL, which again corresponds what rounded above and asymmetric in form. P. to the size range previously chosen for P. herbarum, thurgoviana, on Typha, has larger perithecia on This is the most variable range of Pleospora spores, extended grayish areas of the stem, and more sym and details of form and septation must necessarily metric spores. P. heleocharidis, on grasses and be carefully considered. sedges, shows no discolored areas on the stem and Collections are referred to throughout this paper has spores with more sharply tapered ends and less by number. The data for these numbered collec constriction at the septa. tions are given at the end of the paper. On dicotyledons, P. tomentosa differs in the PLEOSPORA PUNCTATA Wehm. in Lloydia 9: 214. densely tomentose perithecia and P. henningsiana 1946.-lnasmuch as this species has already been has large perithecia of the type found in wood described by the writer, its description will not be inhabiting species, and has large, more cylindric repeated here. The spores (fig. 1) differ from ascospores. those of P. trevoicola in being larger (2~30X10 The transition to the nine-septate condition is 14",), more asymmetric in form and more con seen in P. pleosphaerioides, the ascospores of which stantly seven-septate. The perithecia are small often show a tertiary septum in each of the end (120-150fL) and on small, limited, grayish-colored cells. Its perithecia are strongly setose. areas. In P. minor there are occasional septa in addi Collections: 272 (type) on Scirpus from Wyom tion to the typical seven, showing this same ten ing. dency in a group with somewhat different form of PLEOSPORA THURGOVIANA Wegelin in Mitt. Thur ascospore; i.e., with more rounded ends and more gauischer Natur£. Ges. 12: 9. 1896.-Perithecia 200--400X 200-250"" globose to somewhat de 1 Received for publication October 27, 1951. Papers of the Department of Botany of the University of pressed, immersed beneath the somewhat grayish Michigan No. 962. discolored surface of the stem; grayish areas 3--4 238 AMERICAN JOURNAL OF BOTANY [Vol. 39 Mar<;h, 1952] WEHMEYER~PLEOSPORA 239

TABLE I. Comparison 0/ host, spore, ascus and perithecial measurements 0/ species discussed.

ColI. No. Host Spores Asci Perithecia

p. p. p. Pleospora punctata 272 Scirpus 26-30(33) X 10-14 7S-8SX21-2S 120-1050 Pleospora thurgoviana 271 Typha --_.-- 26-30X9-11 90--140X 1.3-21 300-400 Pleospora heleocharidis 330 Calamagrostis 25-35X9-12.S 90-125X21-26.S 350-400

255 Grass --- 28.5-35X 10-11 115-140X 23-25 300 353 A ira ------32-39X10-11 115-160X23-26 200-350 669 H eleocharis _____ 34-38X 10-11 80-85X 28-31 Pleospora tomentosa

277 Herbs -----~- 23-30X8..5-10.5 100-140X13-20 250-350, T 280 Castilleja _ "------_.--._------28-35X 10.5-12.5 85-108X 19-23 300-400, T 278 Rudbeckia "0. ______• _____ 26-35X9-10 78-9SX20 300-350, T Pleospora pleosphaerioides

273 Gilia ------.------._-_._._--.------32-39X9.5-12 160-200X17-19 400-500,S Pleospora hennuigsiana 283 Stems 39-44X12-14 110-160X20-35 500-700 284 Salix --- 40-44X12-14 140-160X26-35 700-900 Pleospora moravica

282 Salsola ------_.. - 23-26(33) X8-l0 106-140X12.5-14 350-500 489 Vitis -- 26.5-30X9-10 13S-150Xl4-21 350-800 286 Salix --.------26-35X8.5-9.5 140-175X16-21 500-700 Pleospora saponariae 497 Sapon.aria 48-57X 16-18 124X,3S 100-1050 Pleospora minor 276 Grass 2I.5-26.5X7-7.4 8S-170Xl4-19 2050 Pleospora alismatls 285 Alisma 26-33X7-9.5 805-110(60) X12.'s-18 300-0500 Pleospora rubicunda 346 Brachypodium 23-27 (30) X8.'s-1O IlS-130X2I.,S 250-350 287 Aconitum 26-32X9-11 160X21-25 250-350 288 Stems ___ 27-30X8-9 l25-150Xl4-19 200-400 289 Aconitum 30-39X9-12 140X21 300-400 290 Aconitum 32-37X11-13 90-110X21 250-400 P. rubicurula var. americana 0526 Pedicularis 30-38Xl1-12.5 140-160X21-26 250-300 5205 Potentilla 32-37X12's-l's 160 -180X16-21 400 em. in extent; erumpent as scattered papillate osti ity, 90-140X13-31"., imbedded among numerous oles; wall 20-40". thick, of dark brown parenchyma interthecial strips. above, thinner and lighter colored below. Spores (fig. 2) hiseriate to overlapping umserr Asci clavate, with a claw-like base and a slightly ate, fusoid-cylindric to oblong-ellipsoid, mostly thickened wall, becoming more elongate at matur- seven-septate, red-brown, symmetric, inaequilateral

Fig. 1-13.-Fig. I. Ascospores from the type eolleetion (272) of Pleospora punctata Wehm.-Fig. 2. Ascospores from the type collection (271) of Pleospora thurgooiana Wegelin.-Fig. 3. Ascospores from the type collection (669) of Pleospora heleocharidis Karst.-Fig. 4. Ascospores from the type collection (278) of Pleospora tomentosa Wehm. Fig. 5. Ascospores of Pleospora heleocharidis Karst., from collection No. 330 (b) and No. 3053 (c).-Fig. 6. Ascospores from the type collection (273) of Pleospora pleosphaerioides Wehm.-Fig. 7. Ascospores from the type eollection (286) of Pleospora moraviea (Petr.) comb. nov.-Fig. 8. Ascospores from the type collection (276) of Pleospora minor sp. nov.-Fig. 9. Ascospores of Pleospora henningsiana Ruhl., lahn & Paul, from collections No. 283 (a) and '10. 284 (b).-Fig. 10. Ascospores of Pleospora rubicunda Niessl, from collection No. 287 (a) and No. 288 (b). Fig. II. Ascospores from the type eollection (285) of Pleospora alismatis E. & E.-Fig. 12. Ascospores of Pleospora rubicunda val'. americana val'. nov., from collection No. 05205 (a) and the type collection. No. 0526 (h).-Fig. 13. Asco spores from the type eolleetion (497) of Pleospora saponariae (Frag.) comb. nov. 240 AMERICAN JOURNAL OF BOTANY [Vol. 39 or somewhat curved, slightly constricted at the er, in this material, but no clathrate spores were septa, ends bluntly rounded, 26-30X9-11,u. seen. The identity of P. punctiformis is still doubt Collections: 271 (type) on Typha from Switzer ful. land. The descriptions of P. karstenii (P. arctica) and This description is taken from what is apparently P. heleocharidis are very similar, although these type material, although the spores were originally species are supposed to be distinct. The original given as "35-40XI0-14, rarely 26XI0/-t." Nota description of P. heleocharidis gives very large tions on the packet state that it belongs in "Pleospora spores, 45-55X 13-16,u. Berlese (1900) , again punctiformis" and in "Clathrospora," but no indi from type material, gives the spores as 42-48X 13 cation of a clathrate condition of the spores was 15,u. A portion of Karsten's type collection in the seen. This species differs from P. punctata in the Riksmuseum showed a few perithecia but they were larger perithecia and larger discolored areas and all immature. Karsten's Fung. Fenn. No. 882, in in the ascospores being red-brown in color, more the Farlow Herbarium, yielded a few perithecia symmetric in form and having greater constrictions with asci 80-85X 28-311' and seven-septate, yellow at the secondary septa. brown ascospores with bluntly tapered ends and PLEOSPORA HELEOCHARIDIS Karst. in Symbolae constrictions at all septa, measuring 34-44X 10 Mycol. Fennicam 2: 72. 1874.-Perithecia 200 ll,u. The other collections included here differ 400,u in diameter, globose or somewhat depressed, from this exsiccatus only in the slightly smaller often appearing on the surface as small circular ascospores which are more pointed at the ends and black spots with a central, erumpent, papillate osti not constricted at the secondary septa. It is pos ole; wall about 30,u thick, parenchvmatic. sible that varieties on grasses and sedges may be Asci clavate, with thickened walls, base claw-like, distinguished but more collections are needed for 80--140 X 21-31,u. such a decision. Spores (fig. 3, 5) biseriate, fusoid-ellipsoid, seven PLEOSPORA TOMENTOSA Wehm. in Lloydia 9: 213. septate, yellow-brown, symmetric or slightly broad 1946.-This species has also been described by the er above, straight to inaequilateral or slightly writer. It differs from the three preceding ones curved, constricted at the central septum and some on monocotyledons in the densely tomentose peri times slightly so at the other septa, ends mostly thecia. The spores (fig. 4) are fusoid, with rather rather acutely tapered, or sometimes bluntly round blunt ends and not asymmetric. ed, two central cells usually with vulgaris type sec Collections: 277,278 (type), 280; on Castilleja ondary septa, both end cells with secondary trans and Rudbeckia, from the Tyrol and Wyoming. verse septa, 25-44X9-12,u. Rehm's collection (277) from the Tyrol has Collections: 330, 353, 355, 669 (type) ; on Heleo spores which seem to be immature, and rather ir charis, Aira, Calamagrostis and other grasses, from regularly septate, with many septa laid down at an Austria, Finland and Sweden. oblique angle, but otherwise as in the species. The use of this epithet for this group of collec PLEOSPORA HENNINGSIANA Ruhl., Jahn & Paul in tions on grasses and sedges is a provisional one Verh. Bot. Ver. Brandenburg 43: 105. 1901. only. These collections differ from P. punctata and Perithecia large, 500-900X 300-5001', scattered, P. thurgoviana in the lack of discoloration of the somewhat flattened-spheric, entirely immersed in substrate, and in the somewhat larger and more the bark and visible on the surface as small, circu acutely pointed ascospores, They have the larger lar, flattened or sunken spots, commonly with a perithecia of P. thurgoviana and the yellow-brown raised margin and a central erumpent, papillate spores of P. punctata. ostiole; wall stromatic, 20-1001' thick, of coarse There are several described species which must brown parenchyma. be considered in relation to these grass-inhabiting Asci stout-clavate, thick-walled, base claw-like, forms. These are P. karstenii (P. arctica Karst.] , 1l0-160X20-35,u, imbedded in numerous, filiform, P. punctiformis Niessl and P. heleocharidis Karst. interthecial strips. P. punctiformis, as described by Niessl (1876), dif Spores (fig. 9) biseriate, cylindric-ellipsoid, yel fers from these collections only in the rather small low-brown, seven-septate, straight, somewhat asym er spores (23-25X9-11,u) which are given as chest metric, slightly tapered toward one end, both ends nut-brown and oblong. His fig. 10, however, shows rounded, slightly constricted at all septa, vertical a fusoid spore, and he says that the spores are walls in several or all central cells, occasionally in strongly constricted at the central septum and not the end cells, but laid down separately in each cell at the other septa, which is the case in most of these and not of the vulgaris type, sometimes with a collections. Berlese (1900) figures clathrate spores gelatinous envelope, 39-44X 12-14,u. from Niessl's original collection and places this spe Collections: 283, 284; on Salix and other dead cies in Clathrospora. Collection No. 355 is labelled stems from Germany. P. punctiformis var alpina Rehm and has drawings This species is anomalous in many respects. It is of a clathrate, seven-septate spore upon the packet. placed in this series arbitrarily because of the sym There are some collapsed spores which are narrow- metric septation of the spores. The transverse septa March, 1952] WEHMEYER-PLEOSPORA 241 are laid down first, however, as in a leptosphaeroid spores which are five-septate; vulgaris type secon type. The end cells, again, may occasionally have dary septa may arise in the two central cells give vertical septa. It occurs on woody stems and has ing seven-septate spores, or in four of the central the large, thick-walled perithecia of that habitat, cells, giving nine-septate spores. Again, secondary but the spores are biseriate in a clavate ascus. transverse, or true vulgaris septation may appear Collection 283 is accompanied by a full descrip in the end cells, giving rise to an eleven-septate tion and labelled "P. henningsiana W. Ruhl. nov. spore. In this variation within the septation of the sp., exple autoris 5/98, ramis emortuis, prope Pan spore and the large perithecia, this species is simi kow (Berlin)." Sydow's collection (284) is on lar to P. abscondita, which differs only in the more Salix from "Schonhausen bei Berlin, 9/4/98, leg. asymmetric character of its spore septation. Ruhland." The type is given as on Corylus (?), Collection No. 282 is accompanied by a descrip from Wiedershonhausen, Berlin. The two collec tion and the notation "Pleospora conoidea Rehm sp. tions are identical. nov." This is crossed out and the collection is said The transition from the seven- to the nine-septate to differ from P. salsolae in the larger perithecia. condition in the spore, of the general form of that Some of the perithecia have short stout spores found in P. heleocharidis and P. tomentosa, is seen which are seven-septate and very much like those in the following species, which often has tertiary of P. salsolae, except for the lack of vertical septa septa inserted in the end cells. in the end cells. Other perithecia, however, have PLEOSPORA PLEOSPHAERIOIDES Wehm. in Lloydia longer spores with nine or eleven septa. The spores 9: 214. 1946.-This species has been previously in collection No. 489 are nearly all seven-septate, described by the writer. The spores (fig. 6) are but they are obviously immature, and as the peri 32-39X9.5-12.5p. and of the form found in P. thecial structure is identical with Petrak's species heleocharidis and P. tomentosa, being inequilateral, it is included here for the present. It is labelled rather tapered at the ends, constricted at the central "P. coronata," but it is not that species. but not the secondary septa, and red-brown in color. Petrak (1923) states that his species has creep The perithecia differ from those of P. tomentosa in ing tomentum-like hyphae on the surface of the being stiffly setose, rather than tomentose. perithecia, but neither tomentum nor setae were Collections: 273 (type) on Cilia from Wyoming. seen on this material; certainly not sufficient to This increase in the number of septa is carried place it in the genus Pyrenophora, if that genus is much further in the following species, but the spore recognized. form is still similar. The perithecia are of the The following species has a similar spore form, large type found on woody substrata. but larger spores, and a septation which is at first PLEOSPORA moravica (Petr.) comb. nov.-Py definitely leptosphaeroid, but followed by the for renophora moravica Petro in Ann. Mycol. 2: 243. mation of a vulgaris type of secondary septa in the 1923.-Perithecia 350-800X300-600p., immersed central cells, as in the preceding species. in the bark or wood and then erumpent as a stout PLEOSPORA saponarlae (Frag.) comb. nov. papillate ostiole through elliptic; blackened areas Pyrenophora saponariae Frag. in Mem. R. Soc. of the wood surface, or erumpent on decorticated Espanola Hist. Nat. 11: 95. 1919.-Perithecia scat wood as conic ostioles and flattened perithecia; wall tered, 100-150p. in diameter, membranous, im thick, particularly at the sides, 30-120p., composed mersed. Asci about 125X 35p.. of brown to greenish-black parenchyma. Spores (fig. 13) biseriate, fusoid-ellipsoid, dark Asci cylindric-clavate, becoming cylindric, thick red-brown, almost opaque, five- to eleven-septate, walled, particularly at the apex, base claw-like, inaequilateral, flattened on one side, symmetric, 135-175X 14--21p., imbedded in numerous filiform tapered toward the bluntly rounded ends, no con interthecial strips. striction or a very slight one in the middle, pri Spores (fig. 7) biseriate at first then overlapping marily five- then seven-septate, with faint vulgaris uniseriate, fusoid-ellipsoid, variable in length, five type secondary septa appearing in two or four of septate when immature, becoming seven- to eleven the central cells, resulting in nine- to eleven-septate septate by the insertion of vulgaris type septa in the spore, 48-57X 16-18p.. two central, or all the cells, straight or inaequilat Collection: 497 (type) on Saponaria from Spain. eral to slightly curved, constricted at the central This collection is labelled Pyrenophora sapo septum, but not at all or only slightly so at the sec nariae. The perithecia are scattered among the fruit ondary septa, ends tapered, 23-35X8-10p.. bodies of some other fungus, and no setae were Collections; 282, 286 (type), 489; on Salix, Sal seen upon them. The spores, however, are quite sola, Vitis, from Italy, Moravia and the Near East. distinct. The large size, inaequilateral form and There are placed here, under Petrak's species dark color are distinctive. The septation is lepto name a group of collections which have the large sphaeroid. The three primary septa are laid down perithecia and other characters of the wood-inhabit first, then the secondary septa in the end cells, and ing forms and which have extremely variable usually tertiary septa in the resulting end cells, spores. The type collection (286) has immature These first seven septa are usually distinct. A vul- 242 AMERICAN JOURNAL OF BOTANY [Vol. 39 garis type of secondary septation may also occur in thickened, base claw-like, imbedded in numerous, two or four of the central cells, but these walls are filiform, interthecial strips, 85-100 (160IXI2.5 thinner and less distinct. The species is included 18p.. here because of the symmetric septation and spore Spores (fig. 11) biseriate, becoming uniseriate, Iorm which are similar to the preceding species. fusoid to fusoid-ellipsoid, mostly nine-septate, yel The following three species differ somewhat from low-brown, symmetric, ends rounded or bluntly the preceding in their spore form, and are often tapered, inaequilateral or curved, slightly constrict found on reddish or purplish areas of the stem. ed at all septa, two central cells usually with vul The spores have more rounded ends and tend to be garis type septa, remaining cells with or without more inaequilateral or curved than in the preceed vertical walls, no vertical septa in the end cells, ing group. In P. minor and P. alismatis the spores 26--30(33) X7-9.5p.. are also narrowed at the ends and have rather con Collection: 285 (type) on Alisma plantago from spicuous constrictions at all septa, whereas in P. S. Dakota. rubicunda the spores have broader ends and little The original description of this species gives the or no constrictions at all septa. spores (22-27X 10-12 (15) p.) as somewhat shorter PLEOSPORA minor sp. nov.-Perithecia 2S0p. in and broader, but is otherwise identical with the type diameter, scattered, associated with small reddish material seen. The spores have the form of those spots upon the stem. Asci clavate, base claw-like, of P. minor, on grass, but have two extra, tertiary 85-130XI4-19p.. Spores (fig. 8) biseriate above, septa in the end cells. The spores of P. thurgo uniseriate below, fusoid-ellipsoid, seven- (rarely viana also approach those of this species in form, eight-) septate, yellow-brown, inaequilateral or but have blunter ends. slightly curved, symmetric, constricted at all septa, PLEOSPORA RUBICUNDA Niessl in Verhand. Naturf. ends bluntly tapered, the two central cells usually Ver. Brunn 14: 191. 1876.-Perithecia 250-400X with vulgaris type septation, vertical walls in other 200-300p., globose or. somewhat flattened, formed cells scattered, none in the end cells, 21.4-26.5X beneath the surface on purplish-red discolored areas 7-7.5p.. of the stem, soon erumpent as stout papillate osti Collections: 276 (type) on grass from England. oles ; walls 20-30p. thick, of flattened black par This collection (276) is labelled P. rubicunda enchyma. Niessl, but is obviously not that species, although Asci numerous, clavate to cylindric-clavate. thick the reddish spots on the stem might suggest a re walled and with a claw-like base, 90-160XI2-2.5,!, lationship. Only a few perithecia were seen, The imbedded between filiform, interthecial strips. spores are of the same form as those of P. alismatis Spores (fig. 10) biseriate to overlapping uniseri but smaller and with fewer septa. They differ from ate, stout fusoid-ellipsoid, (seven-) usually nine- or the preceding and following species in being nar eleven-septate, yellow-brown to red-brown, sym rower, more strongly constricted at the septa and metric, inaequilateral or curved, constricted at the more strongly curved or inaequilateraJ. central septum, but usually not so at the secondary Pleospora minor sp. nov.-Perithecia 2S0p. dia septa, primarily seven-septate with vulgaris-like sep metro, dispersa, in maculis rufo-purpureis. Asci ta inserted in two to four of the central cells, no clavati, 85-130p. longi, 14-19p. crassi, basi ungui vertical walls in the end cells, 23-39X 8-13p.. culiformes. Sporae sursum biseriatae, deorsum uni Collections 287, 288, 289, 290, 346; on Aconi seriatae, fusiforme-ellipsoidales, 21.5-26.5p. longae, tum, Brachypodium and other stems, from Central 7-7.5p. crassae, 7-(interdum 8-) septatae, lutei Europe. brunnae, inequilaterales vel curvatae, symmetricae, Three of these collections (287, 289, 290) are ad septa constrictae, utrinque late angustate; 2 eel given by Rehm as Pleospora anthyllidis var. aconiti, lulis centralibus cum "vulgaris" septis praedita; but the spores are "cymbiform and 10-11 septate" septis verticalibus in cellulis centralibus dispersis, as in P. rubicunda, not "ovate oblong, 13-15 sep non in cellulis terminalibus. tate," as in P. anthyllidis. All of these collections Specimen typicum (n. 276) vide infra enumera are on purplish-red spots, as given by Niessl for P. tione locorum. In caulibus graminearum, ex An rubicunda. No. 346 has spores with a somewhat glia. variable septation and lighter brown color, ap PLEOSPORA ALISMATIS E. & E. in Jour. Mycol, 8: proaching the variety americana. The spores of this 16. 1902.-Perithecia 300-500X 250-400p., some species have more broadly rounded ends and less what flattened, globose to hemispheric, appearing constriction at the septa than those of P. alismatis. on the surface as rather widely scattered, large, cir Var. americana var. nov.-Perithe<:ia as in the cular to elliptic, black spots, with a central erum species, discoloration of substrate pinkish-purple or pent, papillate ostiole; wall consisting of a thin dark reddish-brown. Spores (fig. 12) 30-38X outer layer of small-celled, brown parenchyma, 20 11-15p., light yellow-brown, slightly or not at all 30p. thick and an inner indefinite layer of hyaline constricted, septation irregular, with tertiary septa parenchyma. successively laid down and the spores, therefore, Asci clavate to cylindric-clavate, wall slightly from seven- to eleven-septate, sometimes asym- March, 1952] AMERICAN JOURNAL OF BOTANY 243 metrically so, with three septa above and five below Berlin, Sept. 4, 1898, leg. W. Ruhland. (Riksmuseet, ex the primary central septum. Herb. Sydow.)-284. P. henningsiana Ruh!. on stems, Pan kow (Berlin), May 1898, with desc, (Hiksmuseet, ex Herb. Collections: 525, 526 (type); on Potentilla and Sydow) (Type) .-285. P. alismatis E. & E. on Alisma Pedicularis, from Washington. plantago, Tacoma Park, S. Dakota, Feb. 1898, leg Griffiths. This variety is erected for two collections from (N.Y.B.G., Griff. W. Arner. Fung. 308) (Type) .-286. Mt. Rainier National Park. Their spores have the Pyrenophora moravica Petro on Salix, Miihr-Weisskirchen: same form as in the species, but may be somewhat Chorin, Aug. 25, 1922, leg. Petrak. (Riksmuseet, Petr. FI. Boh. & Mor. 1696) (Type) .-287. Pleospora anthyllidis more strongly constricted and are lighter, yellow Awd. & Niessl, var. aconiti on Aconitum, Tyrol, Sept. 1905, brown. Their septation arises in the same way as leg. Rehm. (Riksmuseet, ex Herb. Hehm.) .-288. P. rubi in the species, a secondary and tertiary transverse cunda Neissl on stems, bei Neufriedenheim, 1903, leg. septum arising in each primary end cell, and vul Rehm. (Riksmuseet, ex Herb. Rehm.) .-289. P. anthyllidis Awd. & Niess!. var. aconiti Rehm. on Aconitum napellus, garis type septa forming in the four central cells, Taschach Gletscher, Tyrol, Aug., 1875. (Hiksmuseet, ex but these vulgaris type septa are tardily and suc Herb. Rehm.) .-290. P. anthyllidis var. aconti Rehm. on cessively formed and spores can be found with Aconitum, 1885, leg. Prof. Linhart. (Hiksmuseet, ex Herb. seven-, nine- or eleven-septa, whereas in European Rehm.) .-330. Undetermined, on Calamagrostis lanceo collections all eleven septa appear early and simul lata, Sueciae: Jemtlandiae prope viam inter Storlien et StorvaIlen, July 31, 1932, A. G. Eliasson. (Riksmuseet: taneously. In this variety the septation may also Fl. Sueo.) .~346. Undetermined, on Brachypodium uelu be irregular or even asymmetric, or with transverse tina, Klingenteich im Mainecker, May 24, 1909, leg. A. septa which do not extend completely across the Ade. (Hiksmuseet) .-353. Undetermined, on Aira flexuosa, spore. One collection shows a purple-red the other Tome, Lappmark, Vassilaure, Aug. 16, 1903, leg. T. Vester gren. (Hiksmuseet : FI. Suec.) .-355. Clathrospora puncti a red-brown discoloration. [ormis var. alpina Rehm., Tyrol, Sept. 1905, leg. Rehm. Var. americana var. nov.-Perithecia ut in spe (Riksmuseet, ex Herb. Hehm.) .-489. Pleospora coronata cies. Asci clavati, 140-180ft longi, 16-26ft crassi. Niessl on Vitis sp., Italia: Conegliano, July 1879. (La Sporae 30-38ft longae, 11-15ft crassae, pallide lutei Plata Mus. No. 7186).-497. Pyrenophora saponariae Frag. brunneae, non vel paululo constrictae, 7- vel 11-, on Saponaria caespitosa, prope Montree d' Ares (Lerida}, March, 1899, leg. Font. Quer. (Herb. Jard. Bot. Madrid: irregulariter et saepe asymmetrice-septatae. Fung. No. 2920) (Type) inedit. (?) .-525 Pleospora rubi Specimen typicum (n, 526) vide infra enumera cunda var. americana Wehm. on Potentilla flabbeli/olia, tione locorum. In caulibus Pedicularis latifoliae, in Yakima Park, Mt. Rainier, Wash., 6,400 ft., Aug. 18, 1948, republica Washingtonensi. leg. E. G. Simmons No. 1951a (Wehm. Herb,) .-526. P. rubicunda val. americana Wehm. on Pedicularis Iatijolia, COLLECTIONS CITED.~271. Pleospora thurgoviana Weg. on Mt. Rainier, Wash. 6,000 ft., leg. E. G. Simmons No. 1960, Typha lati/olia, Frauenfeld, Feb. 23, 1896, leg. Wegelin. Aug. 17, 1948. (Wehm, Herb.) (Type of var.) .-669. P. (Hiksmuseet, ex Herb. Rehm.) (Typen .--272. P. punc heleocharidis Karst. on Heleocharis palustris, Runsala, tata Wehm. on Scirpus ualidus, Elk Refuge, Jackson, Wyo., June. (Fad. Herb., Karst. Fung. Fenn. 882) (Type). July 1, 1940 (Wehm. Herb. No. 1071) (Type).~273. P. pleosphaerioides Wehm. on Cilia watsoni, south of Teton Pass, Jackson, Wyo., July 11, 1940 (Wehm. Herb. No. SUMMARY 1118a) (Type) .~276. P. rubicunda Niessl on grass, Lynn, This paper concludes the discussion of the genus Anglia, lea. C. B. Plowright, 1878. (Hiksmuscet, ex Herb. Sydow) (Type of P. minor) .~277. P. hispida Niessl on Pleospora by considering the relationships of those stems, Moraine des SuIden Gletscher, July 1889, leg. Rehm. species having spores with more than five trans (Hiksmuseet, ex Herb. Rehm.) .-278. P. tomentosa Wehm. verse septa and with a symmetric, vulgaris type of on Rudbeckia occidentalis, Teton Pass, Jackson, Wyo., septation. One new species, Pleospora minor, and July 11, 1940 (Wehm. Herb. No. 1108) (Type).-280. P. one new variety, Pleospora rubicunda Niessl var. tomentosa Wehm. on Castilleja lineari/olia, Hoback-Snake river junction, Wyo., July 15, 1940. (Wehm. Herb. No. americana, are described. 1159a) .~282. P. concoidea Rehm., inedit, on Salsola rich teri, Buchara prope St. Faral (?), Sept. 1911, leg. Andrs DEPARTMENT OF BOTANY, sow. (Riksmuseet, ex Herb. Rehm. No. 242) .-283. P. UNIVERSITY OF MICHIGAl\, henningsiana Ruh!. on Salix, Park von Schonhausen bei ANN ARBOR, MICHIGAN

LITERATURE CITED BEIlLESE, A. N. 1900. leones Fungorum omnium cogni PETRAK, F. 1923. Mvkologische Notizen. VI. Ann. MycoI. torum ad usum Sylloges Saccardianae accomodates. 21: 182-335. Vol. 2. Typis Serninarii. Patavii. WEHMEYER, L. E. 1952a. Studies in the genus Pleospora. NIESSL, G. VON. 1876. Notizen iiber neue und kritische 11. Lloydia, In press. Pyrenomyceten. Vehr. Naturf. Ver. Brunn. 14: 165 ._--- -. 1952b. Studies in the /!enlls Pleospora. V. In press. 217.