RESEARCH ARTICLE Development and characterization of a new set of genomic microsatellite markers in rice bean (Vigna umbellata (Thunb.) Ohwi and Ohashi) and their utilization in genetic diversity analysis of collections from North East India Banshanlang Iangrai1☯, Arunava Pattanayak1☯¤a*, D. Evanoreen Ann Khongwir1, a1111111111 1 1 1¤b 2 a1111111111 Gratify Pale , Emica Mary Gatphoh , Alpana Das , Nikhil Kumar Chrungoo a1111111111 1 Centre for Biotechnology, ICAR Research Complex for NEH Region, Umiam, Meghalaya, India, a1111111111 2 Department of Botany, North Eastern Hill University, Shillong, Meghalaya, India a1111111111 ☯ These authors contributed equally to this work. ¤a Current address: ICAR-Vivekananda Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand, India. ¤b Current address: CPCRI Regional Centre, Guwahati, Assam, India. *
[email protected] OPEN ACCESS Citation: Iangrai B, Pattanayak A, Khongwir DEA, Abstract Pale G, Gatphoh EM, Das A, et al. (2017) Development and characterization of a new set of Rice bean [Vigna umbellate (Thumb.) Ohwi and Ohashi] is an underutilized crop believed to genomic microsatellite markers in rice bean (Vigna umbellata (Thunb.) Ohwi and Ohashi) and their be domesticated in the Myanmar-Thailand region of Asia. In India, rice bean is mainly culti- utilization in genetic diversity analysis of collections vated in the North-Eastern Hills, which is a hotspot for biological diversity. A 5' anchored from North East India. PLoS ONE 12(7): e0179801. PCR was used to develop microsatellite markers in rice bean. Twenty-eight specific primer https://doi.org/10.1371/journal.pone.0179801 pairs were designed and employed to characterize sixty five ricebean accessions collected Editor: Manoj Prasad, National Institute of Plant from North East India.