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Corporate Sponsors Twelfth Annual Ohio Mass Spectrometry Symposium May 6th, 2015 ______________________________________________________________________________ Corporate Sponsors The Ohio Mass Spectrometry Consortium is pleased to acknowledge the support of the following companies for the 12th Annual Ohio Mass Spectrometry Symposium held at the Ohio Union, The Ohio State University, Columbus, OH Gold Level Thermo Fisher Scientific Waters Corp. Silver Level Advion, Inc. Agilent Technologies, Inc. Bruker Daltonics Page 1 Twelfth Annual Ohio Mass Spectrometry Symposium May 6th, 2015 ______________________________________________________________________________ TABLE OF CONTENTS AGENDA .............................................................................................................. PAGES 3 - 4 2015 OMSS ORAL ABSTRACTS ...................................................................... PAGES 6-28 Nilini Ranbaduge ...............................................................................................6 Whitney Houser ..................................................................................................8 Yi Cai .................................................................................................................9 Sean Harshman ................................................................................................11 Yue Ju ...............................................................................................................13 Lydia Cool ........................................................................................................14 Mellie Paulines ................................................................................................16 Ani Sahasrbuddhe ............................................................................................18 Beulah Solivio ..................................................................................................20 Qiuling Zheng ..................................................................................................22 Andrei Rajkovic ................................................................................................24 Ningxi Yu ..........................................................................................................25 Jing Yan ...........................................................................................................27 2015 OMSS VENDOR WORKSHOP ABSTRACTS ..................................... PAGES 30-34 Thermo Fisher Scientific, Sarah Trimpin ........................................................30 Waters Corp, John P. Shockcor .......................................................................32 Advion, Kelvin Hammond ................................................................................33 2015 OMSS POSTER ABSTRACTS ............................................................... PAGES 35-49 Sunil Badal .......................................................................................................35 Kyle Francis .....................................................................................................37 Allyson Beechy .................................................................................................39 Arpad Somogyi .................................................................................................41 Colbert Miller ..................................................................................................43 Dmytro Kulyk ...................................................................................................45 Arpad Somogyi .................................................................................................47 Aamena Parulkar .............................................................................................49 Page 2 Twelfth Annual Ohio Mass Spectrometry Symposium May 6th, 2015 ______________________________________________________________________________ AGENDA 7:30 – 8:25 am Registration, Continental Breakfast, Posters Set Up 8:25– 8:30 am Opening Remarks/Welcome Arpad Somogyi, The Ohio State University 8:30 – 9:50 am Session I (Chair: Sophie Harvey, OSU) Nilini Ranbaduge (The Ohio State University) Phosphoproteomic identification of oncogenic pathways in LKB1 dependent non-small cell lung cancer by two-dimensional LC-MS/MS Whitney Houser (University of Cincinnati) Combining recombinant ribonuclease U2 and protein phosphatase for RNA modification mapping by liquid chromatography–mass spectrometry Yi Cai (Ohio University) Integration of Electrochemistry with Ultra Performance Liquid Chromatography/Mass Spectrometry (UPLC/MS) Sean Harshman (Wright-Patterson AFB) The Use of Exhaled Breath for the Identification of Hypoxia Biomarkers Ten Minute Break 10:00 – 10:30 am Sarah Trimpin, Wayne State University, for Thermo Fisher Scientific Laserless Matrix-Assisted Ionization-Mass Spectrometry: Rapid Measurements of Small and Large Compounds on Low and High Performance Mass Spectrometers Ten Minute Break 10:40 – 11:40 pm Session II (Chair: Abraham Badu-Tawiah, OSU) Yue Ju (The Ohio State University) Surface induced dissociation ion mobility mass spectrometry localizes multisubunit protein-ligand interactions Lydia Cool (University of Akron) Fragmentation Analysis of Two Structural Isomers Mellie June Paulines (University of Cincinnati) Modified Guanosine at position 34 of placental human tRNA Asp(GUC) and tRNA Asn(GUU) 11:40 – 1:00 pm Complementary Lunch and Poster Session 1:00 – 1:30 pm John P. Shockcor University of Cambridge, Cambridge, UK, for Waters Application to an Investigation of the Effect of Prolonged Glucose Stimulation on the Lipid Profile of Mouse Heart Muscle Page 3 Twelfth Annual Ohio Mass Spectrometry Symposium May 6th, 2015 ______________________________________________________________________________ Ten Minute Break 1:40 -2:40 pm Session III (Chair: Liwen Zhang, OSU) Ani Sahasrbuddhe (The Ohio State University) Surface-Induced Dissociation/Ion Mobility of protein complexes: Interface Area and Subunit Packing Beulah Solivio (University of Cincinnati) Improving RNA Modification Mapping by Mutagenesis of RNase U2 Qiuling Zheng (Ohio University) Development of Electrochemical Cross-Linking Mass Spectrometry for Probing Protein Three-Dimensional Structures and Conformational Changes Ten Minute Break – Afternoon Refreshments (Posters Removed) 2:50 – 3:20 pm Kelvin Hammond, Advion Inc Revisiting the Single Quad Mass Spectrometer. A compact, robust, straightforward and versatile, work horse platform Ten Minute Break 3:30– 4:30 pm Session IV (Chair: Nicholas Brunelli, OSU) Andrei Rajkovic (The Ohio State University) Structural identification by Mass Spectrometry of Cyclic Rhamnosylated EF-P Ningxi Yu (University of Cincinnati) Development and validation of a tandem mass spectral library for modified oligonucleotides from transfer RNAs Jing Yan (The Ohio State University) Surface induced dissociation utilized to characterize protein Complexes trapped in the trap cell of a Q-TOF instrument Page 4 Twelfth Annual Ohio Mass Spectrometry Symposium May 6th, 2015 ______________________________________________________________________________ 2015 OMSS ORAL ABSTRACTS Page 5 Twelfth Annual Ohio Mass Spectrometry Symposium May 6th, 2015 ______________________________________________________________________________ Nilini S. Ranbaduge Department of Chemistry and Biochemistry The Ohio State University [email protected] Phosphoproteomic identification of oncogenic pathways in LKB1 dependent non-small cell lung cancer by two-dimensional LC-MS/MS Nilini S. Ranbaduge1, Joseph M. Amann2, Tadaaki Yamada2, Wang Zhen2, David P. Carbone2, Vicki H. Wysocki1 1Department of Chemistry and Biochemistry, 2Division of Medical Oncology, Department of Internal Medicine, The Ohio State University, Columbus OH Introduction Proteomic profiling of cancer cell lines can be used to identify potentially useful protein biomarkers and to reveal the connectivity between oncogenic pathways. Liver kinase-B1 (LKB1) is a tumor suppressor gene commonly found in 30-40% of non-small cell lung cancer (NSCLC). Somatic mutations of LKB1 lead to loss of its expression and respective key metabolic and regulatory functions. These signaling events are mediated mainly by AMP-activated protein kinase (AMPK). However, the comprehensive mechanism and relationship of LKB1 to NSCLC remains unclear. Here, we present our study on identifying these pathways based on differential regulation of phosphoproteins, and localization of phosphorylation events by label-free proteomics. Methods To study LKB1 signaling events we have used two LKB1-deficient cell lines HCC15, and A549 transduced with a retrovirus carrying wild-type LKB1. Controls were prepared by transducing the cell lines with a vector alone and a kinase-dead version of LKB1. Cell lysates were digested with trypsin in ProteaseMax to generate peptides. The digests were enriched for phosphopeptides with TiO2 columns. The eluate was collected for liquid chromatography coupled to mass spectrometry analysis. The samples were fractionated online into 15-fractions in high pH reverse-phase followed by low pH gradient elution (Waters NanoAcquity). The peptides were analyzed on a Thermo Orbitrap Elite. Preliminary Data This reverse-phase/reverse-phase two-dimensional separation increased the coverage of the phosphoproteome by yielding approximately 4000 more phospho-modified peptides compared to a single 2hr gradient for only 4.5 µg of the sample. In each sample,
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