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Factors Affecting the Vase Life of Rosa Cultivar 'Sonia': Microbiological and Scanning Electron Microscopic Investigations

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Factors Affecting the Vase Life of Rosa Cultivar 'Sonia': Microbiological and Scanning Electron Microscopic Investigations Factors affecting the vase life of Rosa cultivar 'Sonia': Microbiological and scanning electron microscopic investigations Factoren die van invloed zijn op het vaasleven van Rosa cultivar 'Sonia': Microbiologisch en raster electronenmicroscopisch onderzoek CENTRALE LANDB OUW CATALO GU S 0000 0454 3563 Promotor: Dr Ir F. M. Rombouts hoogleraar levensmiddelenmicrobiologie vakgroep levensmiddelentechnologie Henriette M. C. Put Factors affecting the vase life of Rosa cultivar 'Sonia': Microbiological and scanning electron microscopic investigations Factoren die van invloed zijn op het vaasleven van Rosa cultivar 'Sonia': Microbiologisch en raster electronenmicroscopisch onderzoek Proefschrift ter verkrijging van de graad van doctor in de landbouw- en milieuwetenschappen, op gezag van de rector magnificus, dr H. C. van der Plas, in het openbaar te verdedigen op woensdag 15me i 1991 des namiddags te vier uur in de aula van de Landbouwuniverstiteit te Wageningen. V ' . \) S v -, "-'-, ! Roos (foto Judith Tromp) BIBLIOTHEEK ,ANDBOU\ UNIVERSDŒJLB WAGENINGEN HUOÔZDl, Hit STELLINGEN 1. Bij onderzoek naar de invloed van planthormonen op het vaasleven van snijbloemen, dientme n aseptische technieken tehanteren . (ditproefschrift ,hoofdstu k1) . 2. Demicroflor a die zichontwikkel t inhe tvaaswate r van snijbloemenword t bepaald door de ecologische omstandigheden in de vaas en niet door de microflora aanvankelijk aanwezig op destengel . (ditproefschrift ,hoofdstu k2) . 3. De rolva nmicrofungi in snijbloemenvaaswater verdient meer aandacht, (ditproefschrift ,hoofdstu k 1, 2e n4) . 4. Dode-o fgeïnactiveerd emicro-organisme nspele nee nnie tt eonderschatte n rol inhe tvaasleve nva n snijbloemen. (ditproefschrift ,hoofdstu k 1e n5) . 5. Eenroo si see nroos ,i see nroos ,i see nroo s (Stein,E .1931 .In :Befor e the Flowers of Friendship Faded, Faded Friendship Faded). Ditbeteken t ook: Iedereroo sheef thaa r eigenidentiteit ,groeivermogen ,beginbesmetting . Voor statistisch betrouwbaar onderzoek is het noodzakelijk zo homogeen mogelijk uitgangsmateriaal te gebruiken, inhe t bijzonder als voor die onderzoekingen binnen de gegeven tijdsduur van het vaasleven bloemen worden opgeofferd aanperiodie k uit tevoere n analyses. (ditproefschrift ,hoofdstu k3-8) . 6. Deeffecte nva nlag eaantalle nmicro-organismen ,ster kverdund emicrobiël e pectolytische enzymen, en lage concentraties microbiële metabolieten in vaaswater opd ewaterhuishouding ,knopontplooiin g envaasleve nva n Sonia rozen, doen specifieke werkingsmechanismen vermoeden. (ditproefschrift ,hoofdstu k 5, 6e n 7; Halverson, L.J. & Stacey, G. 1986.Microbiol .Rev. , 50, 193-225). 7.Kunstbloeme nverdiene nvoorran g inziekenkamer sva noperatiepatiënte n en intensive care afdelingenva n ziekenhuizen. 8. Het begrip snijbloemvatverstopping door bacteriën wordt menigmaal ten onrechte gebruikt. Dit duidt dan op een verstoorde waterbalans waaraan andere oorzaken ten grondslag liGgen dan bacteriële verstopping van de watertransportbaan van de snijbloem . (ditproefschrift ,hoofdstu k 7; Accati Garibaldi,A . 1983.Act a Horticulturae, 138, 255-260). 9. Microbiologisch onderzoekaa nvaaswate r enstengeldele nva nsnijbloemen , zoals rozen,n a eenvaasleve nva nee nwee ko f langer, geeft geen inzicht in de ontwikkeling van de microflora gedurende de daaraan voorafgaande dagenva nhe tvaasleven ,terwij ljuis tdez ebepalen dzij nvoo rhe tverloo p vanhe t vaasleven. (Aarts, J.F.Th. 1957.Mededelinge n van de LHWageningen , 57(9)1-62). 10. De toepassing van weefselkweekmethoden voor snelle selectie en vermeerdering van siergewassen zowel als van land-, tuin- en bosbouwgewassen, dientbevorder d teworden . 11. -I nd ebijn aeen-en-negenti g jarige geschiedenisva nd eNobelprij svoo r literatuur isvij f maal eenvrou w bekroond. Dit is geenszins eenbewij s dat het literaire oeuvre van vrouwen van minder gehalte is dan datva nmannen . -Ann a Cramer (1873-1968) is niet de enige verzwegen vrouwelijke componist die onze vaderlandse muziekgeschiedenis rijk is. 12. Stat rosapristin a nomine,nomin a nuda tonemus. (Eco,U . 1983. Ilnom e della rosa) Van demen s vanwelee r blijft ons denaam . De geur, de klank, devor m verdwijnen. Naakte namen en het geschreven woord restenons . Stellingenbehoren d bij het proefschrift: Factors affecting thevas e life ofRos a cultivar 'Sonia': Microbiological and scanning electronmicroscopi c investigations. HenrietteM.C . Put Deventer-Wageningen, mei 1991 Stat rosapristina nomine, nomina nuda tenemus. De roos van weleer bestaat als naam, naakte namen houden we over. Uit: Il nome della rosa (Umberto Eco, 1983) Voor: Anton 'A rose is a rose is a rose is a rose' Gertruda Stein (1874-1946) Coverplate: Margaretha Roosenboom (1843-1896)Stil l life with roses OilPain t On Canvas, 50X 6 0cm . References: Hermes,N . 1989.Margareth a Roosenboom In: Bloemen uit de kelder: negen kunstenaressen rond deeeuwwisseling ,40-4 7 Redactie:Oele ,A. , VanRijsingen , M., Vande n Donk, H. Uitgever: Waanders, Zwolle en Gemeentemuseum, Arnhem Defoto' s bij detitelpagin a en bij detitel s van de hoofdstukken 1,8 e n 9zij n van Judith Tromp. Dezefoto' s vormen een deelva n een fotografisch bloemenproject. Defoto' s bij de titels van de hoofdstukken 2-7zij n van de auteur. ISBN90-800045-4- 5 CIP-GEGEVENS KONINKLIJKE BIBLIOTHEEK, DEN HAAG Put. Henriette Maria Christina Factors affecting the vaselif e of Rosa cultivar 'Sonia': microbiological and scanning electron microscopic investigations / Henriette Maria Christina Put. - Deventer :Vijfsprong . -111 . Proefschrift Wageningen. - Met lit. opg. - Met samenvatting in het Nederlands. ISBN 90-800045-4-5 Trefw.: rozen ;microbiologisc h onderzoek. Uitgave: VIJFSPRONG, vrouwenuitgeverij, Deventer, Nederland Kopijrecht berust bij de auteur H. M.C . Put VOORWOORD 'Sonia'rooskleurig eroo s Ind eochten dva nd e 20*apri l198 3reisd ei kva nDevente rnaa rWageningen , bezochthe tSprenge r Instituutaa nd eHaagsteeg ,e nha dee ngespre kme td e directeurdrs .G.J.H .Rijkenbarg .Ee ntweed egespre kvolgde ,di tkee rme td e afdelingshoofdene ncolleg amicrobioloo gY .d eWitte .Ee nderd egespre kzett e puntjeso pd eovereenkoms tdi ewer dgeslote ntusse nd everpakkingsindustri e Thomassen& Drijver-Verblif aN.V .(TDV )t eDevente re nhe tSprenge rInstituu t (SI)t eWageningen . Indez eovereenkoms twer donde rmee rvastgelegd :"TD Vdetacheer tHenriett e "M.C. Put,adviseu rmicrobiologi ee n-technologi evoo rmaximaa l4 dage npe r "weekbi jhe tS Ivoo rhe tverrichte nva nonderzoe kbinne nhe tkade rva nd e "doelstellingen,e ni nopdrach tva nhe tSI .Gemiddel dza lzi jéé nda gpe rwee k "beschikbaar zijn voor haar TDV-functie. Het dienstverband tussen TDV en "Henrietteblijf tvolledi gva nkracht. " Deduu rva ndez eovereenkoms t strektezic hui tva n1 oktobe r 1983to t1 januari 1987, het bereiken van de SUM-gerechtigde leeftijd (Stichting Uittreden Metaalindustrie). Daaropaansluitend werd een persoonlijke overeenkomstgeslote n tussend edirecteu rva nhe tS Ie nmi jzelve ,teneind e publicatie te realiseren van de resultaten van de uitgevoerde reeksen onderzoekingen.Dez eovereenkoms twer dbeëindig do p1 septembe r1988 . Geenroze nO Pd e(spoor)wege ntusse nDevente re nWageninge n Wel:lang ewachttijden ,vuil ewachtkamers ,tochtig eperrons ,i nvoll etreine n publicaties lezend, inslaa p sukkelen,ko uvatten. .Doc hoo konderhoudend e autotochtjes met Natalio Gorin van SI Wageningen naar NS Arnhem, en de donderdagsemaaltijde nme tChristi eBerkhols tbi jDiedenoord' sresto . Door het SJ,wer d gekozenvoo r onderzoek naar de relatie tussenmicro - organismen invaaswate re nhe tvaasleve nva nsnijbloemen ,speciaa l 'Sonia' rozen. De resultaten van dit onderzoek zijn vastgelegd in een 7-tal publicatiesdi ed eker nvorme nva ndi tproefschrift .He tonderzoekpla nkwa m totstan dn acirc a3 maande nliteratuuroriëntatie .He tpla nwer dte rdiscussi e gestelde ngeaccepteer ddoo rrespectievelijk :d eafdelin gfysiologi eva nhe t SI (hoofdO.L . Staden), deadviescommissi e 'Slerteeltprodukten'va nhe tS I (voorzitter J.A.M. Brockhoff) en de contactcommissie houdbaarheid van snijbloemen (voorzitterH .Veen) . Schieteni nd eroo s Bestuderingva nliteratuu rove rsnijbloemen ,vaaswate re nmicro-organismen , wasaanleidin ghe tonderzoekpla nt erichte no penkel eno gweini gbestudeerd e microbiologische factoren in het vaasleven van snijbloemen, en microbiologische methodieken te gebruiken die zelden zijn gebezigd bij onderzoekva nsnijbloeme ne nvaaswater .Di tbetreft :1 .d einitiël ebesmettin g vansnijbloemenstengels ;2 .d ero lva nfung ii nhe tvaasleve nva nsnijbloemen , naast die van bacteriën; 3. het gebruik van gewassen reincultures en gezuiverde of zuivere microblële metabolieten voor de bestudering van mechanismendi eleide nto tee nverkor tvaasleven ;4 .D etoepassin gva nSEM - technieken (Scanning Electron Microscope). Immers, 'verstopping' van xyleemvaten van snijbloemen zou slechts bestaan in de geest van de onderzoeker, tenzijd eonderzoeke rkijk to pd ejuist eplaats ,schrijve nParup s enMolna r (1972), Rasmussen en Carpenter (1974). Een roos op uw hoed Iederepersoo ndi edirec to f indirectheef tbijgedrage n totd e totstandkoming van dit proefschrift wordt een roos op de hoed gestoken. Dit zijn alle medewerkers vanhe t voormalige Sprenger Instituut. In het bijzonder noem ik: medewerkers van de afdeling CMK (Chemie, Microbiologie en Kwaliteit) waarbij ik was ingelijfd en die mij wegwijs maakten; de technische dienst,bibliotheek , fysiologie, statistiek, directie en personeelszaken. Ikdan kpersone n die tekstenkritisc hbekeke n o.a. (B.Boekestein,
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