Dacryodes Edulis) Pulp During Storage Akusu, Ohwesiri Monday*; Wordu, Gabriel Oji and Obiesie Chidiebere
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Akusu, O.M et al., IJFNR, 2019; 3:20 Research Article IJFNR (2019) 3:20 International Journal of Food and Nutrition Research (ISSN:2572-8784) Changes in Microbiological Quality of Table Spreads Produced from African Pear (Dacryodes edulis) Pulp during Storage Akusu, Ohwesiri Monday*; Wordu, Gabriel Oji and Obiesie Chidiebere Department of Food Science and Technology, Rivers State University, Nkpolu Oroworukwo, P. M. B., 5080, Port Harcourt, Nigeria ABSTRACT African pear (Dacryodes edulis) pulp was extracted and *Correspondence to Author: pasteurized. The pasteurized pulp was homogenized with Akusu, Ohwesiri Monday different levels of food grade additives to form table spreads of Department of Food Science and samples A to H while sample I was left without preservative. Technology, Rivers State Universi- The spreads were packed in sealed glass containers and ty, Nkpolu Oroworukwo, P. M. B., stored at room temperature (28±20C) for 4 weeks to evaluate the changes in microbiological quality of table spread during 5080, Port Harcourt, Nigeria storage period. Samples were collected in a weekly interval to study the microbiological assay of the spread starting from week zero to the last week. High total bacteria count of 1.8x107CFU/ How to cite this article: ml was seen in sample I (spread without preservative) at week Akusu O. M, Wordu G. O and zero, this increased significantly to 8.1x108CFU/ml after 3 weeks Obiesie C. Changes in of storage and TNTC (too numerous to count) after 4 weeks of storage at 28±20C. The least growth were observed in samples Microbiological Quality of Table A and C with bacteria counts of 8.1x107CFU/ml and 3.5x107CFU/ Spreads Produced from Af-rican ml, respectively. The least fungi count of 2.0x106CFU/ml was Pear (Dacryodes edulis) Pulp noted in sample C after 4 weeks of storage while the highest during Storage. International Jour- fungi count of 4.5x107CFU/ml was seen in sample I after 4 nal of Food and Nutrition Research, weeks of storage at room temperature (28±20C). The suspected 2019; 3:20. microorganisms based on their morphology were; E. coli, Staphylococcus aureus, Salmonella specie while fungi were Penicillium specie and Aspergillus specie. Deterioration sets in significantly after two weeks storage as total bacteria and fungi counts rose above 1.0x107 and 1.5x106, respectively. The microbiological quality of the samples was stable up to the eSciPub LLC, Houston, TX USA. second week of storage except sample I (without preservative). Website: http://escipub.com/ Keywords: Microbiological Quality, Vegetable Spreads, African Pear Pulp, Storage Temperature, Nutritional Quality, Preservatives IJFNR: http://escipub.com/international-journal-of-food-nutrition-research/ 1 Akusu, O.M et al., IJFNR, 2019; 3:20 1. Introduction pulp and its oil [14] and the oil extraction processes[15]. These studies revealed Food preservation is the science of extending excellent nutritional qualities of fruit pulp and the shelf life of food, maintaining its nutritional interesting food processing properties of the quality and avoiding the growth of unwanted oils extracted from the pulp and kernel safou microorganisms [1]. It is the process of treating [16]. These have also revealed the importance and handling food to stop or slow spoilage of this fruit nutritionally, therapeutically and in which results to loss of quality, edibility or cosmetics. The pulp, the only edible part of the nutritional values and thus allow for longer fruit is particularly rich in lipids, hence, it could storage. Preservation involves preventing the be an important source of oil [17]. Besides growth of bacteria, yeast, fungi and other lipids, D. edulis pulp contains substantial microorganisms [2]. Vegetable spreads are amount of many other nutrients including spreadable product having at least 90% proteins, carbohydrates, minerals, vitamins and ingredients from nuts and pulps used in various fibres[16]). forms, such as paste and slurry [3,4]. Vegetable spreads are used like the commercially High content of protein and other essential available butter, they can be produced from nutrients in African pear spread makes it almond, cashew, hazelnut, macadamia nut, susceptible to microbiological contamination peanut, pecan, pistachio and walnut [5]. and growth. Thus, the objective of this work Vegetable spread is popular and widely was to produce table spreads from African pear accepted by consumers due to its flavour, good pulp treated with different levels of nutritional values and suitability for preservatives and to evaluate the effect of room 0 consumption either alone or in combination with temperature (28±2 C) storage on the a variety of other foods. African pear pulp has microbiological quality of the product. great potential for vegetable spread production. 2. Materials and Method The African pear tree (Dacryodes edulis) is a 2.1. Materials tropical oleiferous fruit tree that possesses Mature and good quality fruits of the African enormous potential in Africa [6]. Various parts pear (Dacryodes edulis) were purchased from of the tree are used in traditional medicine[7,8]. the fruit market in Port Harcourt and transferred The wood serves for firewood and carpentry [9]. to the Microbiological Laboratory in the The entire tree is used in Agro-forestry systems Department of Food Science and Technology, for soil conservation [10]. Dacryodes edulis fruit Rivers State University, Port Harcourt, Nigeria. is popular in the diets of many Africans. It can All apparatus and chemicals used for the study be eaten raw, roasted or boiled in hot water and were obtained from the same Laboratory and is eaten alone or used in garnishing cooked or were of analytical grade. roasted maize. It could also be used as butter 2.2. Extraction of African Pear Pulp and the to eat bread [8]. It has been found in Cameroon Formulation of the Spread recipe that when the pulp of African pear is cooked and seasoned, it serves as spread The pulp was extracted using modified [11]). According to Ayuku et al.[12], D. edulis traditional method of pear roasting. African pear has a potential to improve nutrition and food fruits were sorted and washed with tap water security. During the last three decades, more and sodium chloride solution, furthermore 0 and more studies have been conducted on D. rinsed thoroughly. They were roasted at 60 C edulis, essentially the tree and its fruit. The for 4 min in a hot air oven (model QUB scientific researches on D. edulis focused on 305010G, Gallenkamp, UK). The roasted fruits the characterisation of propagation techniques were allowed to cool for 10 min, the thin bluish- of D. edulis tree [13], the nutritive value of its black epicarp were gently removed and IJFNR: http://escipub.com/international-journal-of-food-nutrition-research/ 2 Akusu, O.M et al., IJFNR, 2019; 3:20 discarded while the soft pulps were scraped off The mixture was homogenized properly using a and recovered in sterile stainless steel plates. laboratory stirrer (model JKL 2145, REMI The extracted pulp was pasteurized by heating Motors, India) to produce the African pear at 1000C for 5 min in a stainless pot. The spread (APS) as presented in Figure 1 and pasteurized pear pulp was treated with different Table 1. levels of recommended food preservatives [18]. African Pear (Dacryodes edulis) Fruit Sorting/Cleaning Oven roasting (600C for 4 min) Cooling (10 min) Peeling (Recovery of pulp from the hard central core) Pasteurizing (1000C for 5 min) Homogenizing (with addictives/preservatives) African Pear Spread Figure1. Flow Chart for the Production of African Pear Spread Table 1. African Pear Spread (APS) Formulations SAMPLES PEAR PULP H2O PS (E200) BHT (E321) CA (E330) (g) (g) (g) (g) (g) A 100 15 0.045 B 100 15 0.035 C 100 15 0.01 D 100 15 0.005 E 100 15 0.008 F 100 15 0.005 G 100 15 0.025 0.005 H 100 15 0.005 0.008 I 100 15 Key: PS = Potassium sorbate, BHT = Butylated hydroxytoluen, CA = Citric acid. 2.3. Storage of Samples from week one, two, three and four using week The produced spreads were stored in sealed zero as the control sample. glass containers at room temperature (28±20C). 2.4. Microbiological Analysis Samples were collected at one-week interval Storage stability of the spreads were conducted for a period of four weeks for storage studies to study the changes in microbiological quality which included; total bacterial count, total fungi IJFNR: http://escipub.com/international-journal-of-food-nutrition-research/ 3 Akusu, O.M et al., IJFNR, 2019; 3:20 count and an assay of the microorganisms using the spread plate (surface plating) method. associated with the spoilage of African pear The five tubes (10-5) were used for inoculation. spreads dusring storage. From each of these tubes, 0.1ml was pipette 2.4.1. Total Bacteria and Fungi Count onto the surface of an agar plate and spread evenly using a sterile L-shaped glass spreader. Microbial assay was carried out on each of the The plates were incubated at 350C for 24 - 48 stored spread samples to test the presence of hr for NA and 300C for 96 hr for PDA. The bacteria and fungi using nutrient agar (NA) and colonies were counted using a colony counter potato dextrose agar (PDA) for total bacteria and result recorded as colony forming unit per and fungi counts, respectively. The procedure ml (CFU/ml). described by the International Commission on Microbiological Specification for Food [19] was 2.4.5. Isolation and Identification of adopted in this study. Microorganisms Associated with the Spoilage 2.4.2. Sterilization of Materials This was done through sub-culture to obtain All glassware used were sterilized by pure culture of isolates. Single colony of autoclaving at 121oC for 15 min and allowed to bacteria was randomly isolated from the mother cool before use.