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Sphaerotilus Natans, Une Bactérie Filamenteuse Et Neutrophile Avec Une Relation Avec Le Fer : Mecanismes De Piégeage D’Uranium Marina Seder-Colomina

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Sphaerotilus Natans, Une Bactérie Filamenteuse Et Neutrophile Avec Une Relation Avec Le Fer : Mecanismes De Piégeage D’Uranium Marina Seder-Colomina Sphaerotilus natans, une bactérie filamenteuse et neutrophile avec une relation avec le fer : mecanismes de piégeage d’uranium Marina Seder-Colomina To cite this version: Marina Seder-Colomina. Sphaerotilus natans, une bactérie filamenteuse et neutrophile avec une re- lation avec le fer : mecanismes de piégeage d’uranium. Earth Sciences. Université Paris-Est, 2014. English. NNT : 2014PEST1070. tel-01127438 HAL Id: tel-01127438 https://tel.archives-ouvertes.fr/tel-01127438 Submitted on 7 Mar 2015 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Joint PhD degree in Environmental Technology 'RFWHXUGHO¶8QLYHUVLWp3DULV-Est 6SpFLDOLWp6FLHQFHHW7HFKQLTXHGHO¶(QYLURQQHPHQW Dottore di Ricerca in Tecnologie Ambientali Degree of Doctor in Environmental Technology Thèse ± Tesi di Dottorato ± PhD thesis Marina SEDER-COLOMINA Sphaerotilus natans, a neutrophilic iron-related filamentous bacterium: M echanisms of uranium scavenging To be defended December 1st, 2014 In front of the PhD committee: Prof. A. KAPPLER Reviewer Prof. P. BAUDA Reviewer Dr. J-J. PERNELLE Examiner Prof. P. LENS Examiner Dr. G. MORIN Promotor Prof. S. ROSSANO Promotor Dr. Hab. G. ESPOSITO Co-Promotor Dr. Hab. E.D. VAN HULLEBUSCH Co-Promotor Erasmus Joint doctorate programme in Environmental Technology for Contaminated Solids, Soils and Sediments (ETeCoS3) The first to arrive, the last to leave, and in the meanwhile, a source of inspiration for our own evolution Acknowledgments I would like to thank the European Commission for providing financial support through the Erasmus Mundus Joint Doctorate Programme ETeCoS3 (Environmental Technologies for Contaminated Solids, Soils and Sediments) under the EU grant agreement FPA n° 2010-0009 and the French Ministry of Foreign Affairs in the framework of MOY Programme under Moy Grant n°2010/038/01. I would also like to acknowledge the members of the Environmental Technologies for Contaminated Solids, Soils and Sediments (ETeCoS3) committee: Dr. Hab. Eric VAN HULLEBUSCH, Université Paris Est, UPE (France); Dr. Hab. Giovanni ESPOSITO, Università degli studi di Cassino e del Lazio Meridionale, UNICLAM (Italy) and Prof. Piet LENS, UNESCO-IHE (The Netherlands) for giving me the opportunity to participate to this joint Erasmus Mundus doctorate programme, helping me during PhD mobility and most importantly, for scientific discussions and comments specially during the fantastic summer schools. I would also like to thank the two main institutes that hosted me during these three years, the Institut de Minéralogie, Physique des Matériaux et de Cosmochimie – Université Pierre et Marie Curie, IMPMC – UPMC (France) and the Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture, Irstea (France) for giving me the chance to conduct the experimental part of my PhD research in their laboratories. I would like to deeply thank Dr. Guillaume MORIN, co-promotor of my PhD thesis in the IMPMC – UPMC for his help and inspiration during these three years. I would specially like to thank him for showing me that science is always interesting, no matter the subject studied. Indeed, he successfully introduced me into the synchrotron-based sciences even if I did not know about their existence before this PhD. I will be always grateful also to Dr. Jean-Jacques PERNELLE, my advisor in the Irstea, who has mentored me during all this time, giving me valuable advices for the molecular biology section of my PhD thesis and showing me the kindest face of science. In addition, I would like to thank Dr. Georges ONA-NGUEMA and Dr. Karim BENZERARA (IMPMC – UPMC) for their fruitful discussions, specially concerning all geomicrobiology aspects of my work. I would specially like to thank Dr. Alex GÉLABERT from the Institut de Physique du Globe de Paris, IPGP – Université Paris Diderot (France) and also Dr. Luigi FRUNZO from the Università degli studi di Napoli Federico II, UNINA (Italy) for their nice introduction on modelling. I would like to express my gratitude to Prof. Stéphanie ROSSANO (UPE) for her kind words, advice and scientific discussions that helped me so much during this PhD. I would also like to thank the external members of the jury: Prof. Andreas KAPPLER, Universität Tübingen (Germany) and Prof. Pascale BAUDA, Université de Lorraine (France) that took the time to read and evaluate my PhD work. I would like to extremely thank the scientific staff that has participated in my research. I have a special merci for Jessica BREST and Fériel SKOURI-PANET (IMPMC – UPMC) and Anne GOUBET (Irstea), the three main pillars of my research. Without these three wonderful women nothing of this could have been possible. In addition, I would like also to thank Céline FÉRARD, Sylvain LOCATI and Mélanie POINSOT for their help in the experimental setup. Thanks to the MEB-Team, Imène ESTEVE and Sébastien CHARRON (IMPMC – UPMC) for introducing me in the passionate world of Scanning Electron Microscopy. I also thank Benoît CARON [Institut des Sciences de la Terre de Paris, IsTeP – UPMC], Emmanuel AUBRY [Milieux environnementaux, transferts et interactions dans les hydrosystèmes et les sols, METIS – UPMC] and Mickael THARAUD (IPGP) for their help in ICP-MS/OES and F-AAS analyses. I thank also Olivier MATHON and Dipanjan BANERJEE for their support during XAS data acquisition at BM23 and BM26A (DUBBLE) beamlines respectively at the ESRF (France). I would like to thank now my colleagues and friends from the different places I have been during these three years, starting for those who moved as much as I did, so thanks to all ETeCoS3 students. I would like to specially acknowledge Nang for all the moments we spent together in Paris, Rohan for his always valuable advice and for the scientific discussions and collaborations that I hope will continue long time and Joana for her kind words under any circumstance. I thank the Italian team, Maria, Borislava, Anish, Flavia, Marta and Nik for all their help during the first days and the good moments spent there. Grazie mile a Fabrizia, Rosalia e Alessia per il loro aiuto e la loro ottima cucina napoletana. Moltes gràcies a totes les valencianes, Balma, Maite, Ana, Luisa, Inma, Maria, Alex, Ares i Guifré (valencians del nord) amb qui he compartit tants moments durant estos anys, i qui m’han fet tocar de peus a terra quan la recerca començava a ser massa dura. Gràcies/Merci à Malu de m’avoir appris le français avec beaucoup de patience, pour ses conseils et les soirées sushi. Merci aussi à Yohann, Jérôme et Florent pour les deux années de coloc à Bagnolet, la première maison que j’ai eu en France. Gràcies als biòlegs que van vindre des de Barcelona només per vore a presentació. Gràcies/Merci à Nicolas, pour son soutien, toujours, mais surtout pour celui pendant la préparation de l’oral. A la meua família (ma mare, mon pare, ma germana, Dolça i Roser) que des de la distància sempre m’ha donat suport per continuar endavant. Je tiens à remercier mes collègues de l’Irstea : Ahlem, Julien, Pierre, David, Sylvain et Yoan pour leur convivialité pendant ces trois années, les matchs de volley et les Friday Drinks. Un merci spécial pour Nadège, pour être toujours souriante et de qui j’ai appris tellement de choses. Je voudrais remercier aussi mes collègues de l’IMPMC pour les bons moments passés à la cantine (surtout la première année), la cafèt (résistance anti-cantine à partir de la deuxième), et les différentes sorties, voyages et bars qu’on a partagés pendant ces trois années. Je voudrais remercier spécialement l’équipe MINENV et toutes ses générations. Merci à ceux qui ont initié le lignage, la toujours souriante Gaby ; le collègue de concerts Guillaume Othmane ; Fabien, toujours omniprésent dans les conversations ; Delphine, qui ne s’arrête jamais pour rien ; Vincent, exemple de bon scientifique et meilleur être humain ; Areej, le courage en personne et que j’admirerai toujours ; Maya, toujours le bon mot à l’instant précis ; Pierre, calme et sage pour les moments importants et spécialement, je remercie Sandy Ardo (a.k.a. Tic) pour tout, absolument tout, les bons moments, les mauvais, l’accueil pre-aéroport chez elle quand je bougeais entre l’Italie et la France, le voyage merveilleux au Liban, et surtout, son amitié et son soutien au cours de ces trois années. Table of Contents Table of Contents ................................................................................................................. I! List of Figures ..................................................................................................................... V! List of Tables ....................................................................................................................... X! List of Abbreviations ....................................................................................................... XII! Abstract ........................................................................................................................... XIV! Résumé .............................................................................................................................
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