DOI : 10.35124/bca.2019.19.S1.2561 Biochem. Cell. Arch. Vol. 19, Supplement 1, pp. 2561-2569, 2019 www.connectjournals.com/bca ISSN 0972-5075

EFFECT OF SALT STRESS ON SOME CALLUS CHARACTERISTIC OF CORIANDRUM SATIVUM L. IN VITRO

Shurooq Falah Hasan*, Muhson Chillab Abbas and Fadel Kadhim Kareem Faculty of Science, University of Kufa, Iraq. *e-mail : [email protected] (Received 12 March 2019, Revised 24 May 2019, Accepted 9 June 2019)

ABSTRACT : The study included the effect of NaCl salt concentrations on callus characteristics. It included sterilization of explants (Coriandrum sativum L.) and cultured on MS medium containing BA 1 mg /l and NAA 0.5 mg / l. Effect different levels of the salt NaCl (0, 2, 4g/l) were tested on culture medium (MS) to investigate their influence on callus growth and characteristics . NaCl salt treatment at 2g\l significant increase callus fresh and dry weight and protein content .While proline content, total soluble carbohydrate ,catalase enzyme (CAT) and superoxide dismutase (SOD) the significantlyincrease when the salt concentration increase. Key words : Coriander , tissue culture, salinity, fresh weight, dry weight, antioxidant enzyme, protein, carbohydrate, proline.

INTRODUCTION costs of the production (Kadhim, 2016). Coriander (Coriandrum sativum L.) is one of the Classification of coriander (Coriandrum sativum L.) chief medicinal , it has place to the Umbelliferae The taxonomical position was as the following: family () (BenFredj, 2013). Is one of the Kingdom: Plantae – . important it used in medicin, food , spice purposes and in the pharmacy (Bonab et al, 2015). Also, coriander is used Sub kingdom : Ttacheobionta – Vascular plants. headache, digestive, liver stimulant, astringent, anti- Super division: Spermatophyta – Seed plants. diabbetic, anthelmintic, diuretric, anti- pyretic, dyspeptic, Division: Angiospermae – Flowering plants. anti-inflammatory, stomatit, haemostat, conjuctivit, headache, appetizer. Diaphoretic activity so it is used in Class: Magnoliopsida – Dicotyledons. disorders of respiratory and urinary systems in the Indian Sub class : Rosidae. traditional medicine (Oudah and Ali, 2010). Salt stress Order:- Umbellales. cause cellular dehydration, osmotic stress and remove of Family: Apiacae – Carrot family. water from the cytoplasm resulting in a decrease of the cytosolic and vacuolar volumes. Salinity often generates : Coriandrum sativum L. coriander both ionic and osmotic stress in plants, resulting in The classification of Coriandrum sativum L. accumulation or decrease of definite secondary depending on Priyadarshi and Borse (2014). metabolites in plants (Akula and Ravishankar, 2011; Coriandrum sativum L have place family Madloom, 2016). NaCl is one of the greatest famous cause Umbllifereae (Apiaceae) (Verma et al, 2011) as annual used in priming on certain crops like wheat and herb plant originally from the Mediterranean region and watermelon (Sivritepe et al, 2003). MidEastern (Rahimi et al, 2013; Said et al, 2014), it is In vitro tissue culture is a beneficial tool to study the small sized anew formed leaves are lesser herb having cellular processes of the stress and its application at the several branches and sub branche (Nimish et al, 2011). cellular level, independent of regulatory mechanisms There original leaves are ovoid in shape but aerial leaves happening at the all plant level. As wall as though a little are extended shape, there flowers are white, fruit are examples of commercial production by culture of plant round in shape and divided into two round parts. cell, still a countless number from expensive secondary Plant Distribution metabolites are not possible to be produced by plant cell Coriander (Coriandrum sativum L.) is distributed cultures due to the low productivity and high procedure