DNA Research, 2018, 25(1), 25–37 doi: 10.1093/dnares/dsx035 Advance Access Publication Date: 18 September 2017 Full Paper Full Paper Genome sequencing and comparative genomics reveal the potential pathogenic mechanism of Cercospora sojina Hara on soybean Xuming Luo1,2,†, Jidong Cao1,†, Junkai Huang1,2,†, Zongyi Wang3, Zhengyan Guo4, Yihua Chen4, Shumei Ma5,*, and Jun Liu1,2,* 1State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China, 2University of Chinese Academy of Sciences, Beijing 100049, China, 3Beijing Key Laboratory of Agricultural Product Detection and Control for Spoilage Organisms and Pesticides, Beijing University of Agriculture, Beijing 102206, China, 4State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China, and 5Department of Plant Protection, College of Agriculture Resources and Environment, Heilongjiang University, Harbin 150080, China *To whom correspondence should be addressed. Tel. þ010 6480 6131. Email:
[email protected] (S.M.);
[email protected] (J.L.) †These authors contributed equally to this work. Edited by Dr. Satoshi Tabata Received 21 May 2017; Editorial decision 13 August 2017; Accepted 16 August 2017 Abstract Frogeye leaf spot, caused by Cercospora sojina Hara, is a common disease of soybean in most soybean-growing countries of the world. In this study, we report a high-quality genome sequence of C. sojina by Single Molecule Real-Time sequencing method. The 40.8-Mb genome encodes 11,655 predicated genes, and 8,474 genes are revealed by RNA sequencing. Cercospora sojina ge- nome contains large numbers of gene clusters that are involved in synthesis of secondary metab- olites, including mycotoxins and pigments.