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Diversidad Morfo-Genética De Cercospora En Soja

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Diversidad Morfo-Genética De Cercospora En Soja Latorre Rapela, María Gabriela de los Milagros - 2013 - UNIVERSIDAD NACIONAL DEL LITORAL Facultad de Bioquímica y Ciencias Biológicas Tesis para la obtención del Grado Académico de Doctor en Ciencias Biológicas Diversidad morfo-genética de Cercospora en soja. Detección precoz de la infección por C. kikuchii Bioq. María Gabriela de los Milagros Latorre Rapela Director de Tesis: Dra. María Cristina E. Lurá Co-director de Tesis: Dr. Iván S. Marcipar Lugar de realización: Cátedra de Microbiología General Facultad de Bioquímica y Ciencias Biológicas Universidad Nacional del Litoral -2012- Latorre Rapela, María Gabriela de los Milagros - 2013 - A mi familia… MARIA GABRIELA LATORRE RAPELA i Latorre Rapela, María Gabriela de los Milagros - 2013 - Deseo expresar mi profundo agradecimiento: A la Universidad Nacional del Litoral por haberme dado la oportunidad como docente de dicha casa de estudios, de crecer en mi formación científica, por el otorgamiento de la beca y el financiamiento de los proyectos que permitieron la realización de mis estudios doctorales. A la Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) por haber subsidiado parte del Proyecto PICTO 2003, lo cual fue de gran ayuda para seguir adelante con mi trabajo de tesis. A la Facultad de Bioquímica y Ciencias Biológicas por haberme permitido hacer uso de sus labora- torios y de todo el material e instrumental que en ellos se encuentran, gracias a lo cual pude llevar a cabo cada uno de los objetivos planteados en esta tesis. Al personal de la oficina de posgrado de la Facultad de Bioquímica y Ciencias Biológicas, muy especialmente a Adriana, Gachi, Anita y Luciana por todo su apoyo durante estos seis años, por estar siempre dispuestas a responder mis consultas, por su buena predisposición y por el cariño que siempre me brindaron. A los miembros del Jurado, por haber aceptado formar parte del mismo y por el interés y tiempo dedicado a la lectura de este trabajo. Muy especialmente a mi Directora, la Dra. María Cristina Lurá, por confiar en mí, por permitirme abrazar un sueño, por acompañarme incansablemente durante todos estos años, por su generosidad al brindarme sus conocimientos y su experiencia científica, por sus valiosas sugerencias y acertados aportes durante la escritura de esta tesis, por su presencia incondicional y desinteresada. Muy especialmente al Dr. Iván Marcipar por haber aceptado ser mi Co-director aún sin conocerme, por poner a mi disposición su laboratorio y todo su equipamiento, por su generosidad científica y valiosas críticas al discutir los resultados de este trabajo, por guiarme y enseñarme a trabajar en un tema desconocido, hasta ese momento, para mí. Por haber estado presente, incondicionalmente, siempre que lo necesité. A los integrantes del Programa RiiA por sus valiosos aportes en la recolección y envío de las mues- tras sin las cuales este trabajo no hubiese sido posible. A los docentes de la Cátedra de Fitopatología, especialmente a la Ing. Agr. Roxana Maumary por sus importantes aportes en el conocimiento de las enfermedades de la soja, por su colaboración en el modelo experimental de infección de plantas de soja por especies de Cercospora, y por su buena predisposición. A la Ing. Agr. Margarita Sillón por haber sido quien me enseñó a reconocer por primera vez la mi- cromorfología del hongo Cercospora y cedió amablemente las primeras plantas de soja con sinto- matología del tizón de la hoja. A los docentes del Departamento de Matemáticas: M.Sc. Elena Carrera, Lic. Stella Vaira y Lic. Mauro Colombini por sus valiosos aportes en el análisis estadístico de los resultados obtenidos en la caracterización molecular de los aislamientos fúngicos y por su buena predisposición siempre. MARIA GABRIELA LATORRE RAPELA ii Latorre Rapela, María Gabriela de los Milagros - 2013 - A la Dra. Raquel Chan por haber guiado mis primeros pasos en el camino de la biología molecular y haber puesto a mi disposición sus conocimientos y su laboratorio. Al Dr. Agustín Arce por haber brindado su tiempo y sus conocimientos en el análisis estadístico de algunos de los resultados obtenidos en la caracterización molecular de los aislamientos fúngicos. A mis queridos compañeros de la Cátedra de Microbiología General: Marice, Fernanda, Marina, José, Ayelén, Martín, Ludmila, Moni, Romina y Erica porque siempre me apoyaron, contuvieron, me alentaron, me escucharon, me aconsejaron, se alegraron de mis logros, cuando fue necesario me reemplazaron en mi actividad docente. Demostraron ser un verdadero grupo de trabajo, con un fuer- te compromiso hacia el otro, a pesar de nuestras diferencias. Sus aportes, pocos o muchos, fueron oportunos, útiles y necesarios para que yo continúe avanzando por este camino. Al Titular de la cátedra de Parasitología y Micología Bioquímico Pedro Sarsotti, por haberme brin- dado todo su apoyo a lo largo de estos años. Al Dr. Guillermo García por aclarar mis dudas siempre que lo necesité. A los docentes, becarios, cientibecarios y pasantes del Laboratorio de Tecnología Inmunológica: Cristina, Carolina, Nazarena, Iván, Gabriel, Ariel, Cecilia, Verónica, Alejandro, Lucila, Melina, Antonella y Natalia por haberme hecho sentir una integrante más del grupo de trabajo, por la ayuda desinteresada brindada en todo momento, por las risas compartidas entre ensayos de ELISA y geles de poliacrilamida, por el cariño demostrado siempre y por tantos momentos agradables que me hi- cieron pasar. Al Bioquímico Mauricio Santos, quien fuera mi cientibecario, por su destacada colaboración. A la Dra. Valeria Iacona y M.Sc. Ana María González por haberme enseñado, guiado, aconsejado, por haber sido partícipes de mi formación tanto en docencia como en investigación y por haber te- nido el placer de compartir muchos años de trabajo juntas. A todo el personal de la FBCB, quienes cumplen en silencio tareas muy valiosas y necesarias, día tras día, para que como docente e investigadora, pueda llevar adelante mi trabajo. Al grupo Misionero “San Francisco Javier” porque sus oraciones me dieron la fuerza necesaria para seguir adelante. A mi familia que me acompañó y apoyó durante todos estos años, soportando muchas veces mi mal humor. A mis amigas de toda la vida y las que me regalaron mis hijos, con quienes compartí y comparto felices momentos. A Dios y a la Virgen de los Milagros. María Gabriela MARIA GABRIELA LATORRE RAPELA iii Latorre Rapela, María Gabriela de los Milagros - 2013 - Los resultados de esta Tesis doctoral fueron difundidos a través de los siguientes trabajos científicos: Lurá, M.C.; Di Conza, J.A.; González, A.M.; Latorre Rapela, M.G.; Turino, L.; Ibañez, M.M. e Iacona, V. (2007) Detección de variabilidad genética en aislamientos de Cercospora kikuchii con- taminantes de un mismo sembradío de soja. Rev. Argent. Microbiol. 39: 11-14. González, A.M.; Turino, L.; Latorre Rapela, M.G. y Lurá, M.C. (2008) Cercospora kikuchii aislada en la provincia de Santa Fe (Argentina): variabilidad genética y producción de cercosporina in vitro. Rev. Iberoam. Micol. 25: 237-241 Lurá, M.C.; González, A.M.; Herzog, L.; Latorre Rapela, M.G.; Machuca, L.; Mancipar, I.; Mau- mary, R.; Pioli, R. (2009) Cercospora kikuchii aislada de soja. Variaciones morfológicas y genéti- cas. Medidas de prevención y detección precoz de la infección. INTA- Estación Experimental Agropecuaria Rafaela- Información Técnica de cultivos de verano. Campaña 2009 115:124-127. Lurá, M.C.; Latorre Rapela, M.G.; Vaccari, M.C., Maumary, R.; Soldano, A.; Mattio, M.; Gonzá- lez, A.M. (2011) Genetic Diversity of Cercospora kikuchii Isolates From Soybean Cultured in Ar- gentina as Revealed by Molecular Markers and Cercosporin Production. Mycopathologia 171: 361-371. Capítulo de libro Latorre Rapela, M.G.; Colombini, M.A.; González, A.M.; Vaira, S.M.; Maumary, R.; Mattio, M.C.; Carrera, E. y Lurá, M.C. (2011) Phenotypic and genotypic variability in Cercospora kikuchii isolates from Santa Fe province, Argentina. En: Soybean – Genetics and Novel Techniques for Yield Enhancement (Ed.: Krezhova D.) INTECH. Croacia, p.97-112. ISBN 978-953-307-721-5 Congresos Lurá, M.C.; Di Conza, J.A.; González, A.M.; Latorre Rapela, M.G.; Vaccari, M.C.; Turino, L.; Iba- ñez, M.M.; Mattio, M.C.; Benzzo, M.; Iacona, V. Variabilidad genética en cepas de Cercospora kikuchii. 3º Congreso de Soja del Mercosur. Rosario, Argentina, del 27 al 30 de Junio de 2006, p. 311. González, A.M.; Turino, L.; Astegiano, E; Maumary, R. Latorre, M.G.; Culzoni, M.J.; Porto, A.; Iacona, V.; Lurá, M.C. Cercospora kikuchii: producción de cercosporina in vitro. Su relación con los genotipos. 3º Congreso de Soja del Mercosur. Rosario, Argentina, del 27 al 30 de Junio de 2006, p. 318. Turino, L.; Lurá, M.C.; Latorre Rapela, M.G.; González, AM. Cercospora kikuchii aislada en la provincia de Santa Fe (Argentina): variabilidad genética y producción de cercosporina in vitro. XI Congreso Argentino de Microbiología. Córdoba, Argentina, 10 al 12 de Octubre de 2007, p. 145. MARIA GABRIELA LATORRE RAPELA iv Latorre Rapela, María Gabriela de los Milagros - 2013 - Vaccari, M.C.; Latorre Rapela, M.G.; Lurá, M.C. Cercospora kikuchii. Detección de su variación genética a través de marcadores moleculares. XI Congreso Argentino de Microbiología. Córdoba, Argentina, 10 al 12 de Octubre de 2007, p. 149. Latorre Rapela, M.G.; González, A.M.; Lurá, M.C. Caracterización fenotípica y molecular de Cer- cospora kikuchii aislada de soja en la provincia de Santa Fe. XI Congreso Argentino de Microbio- logía. Córdoba, Argentina, 10 al 12 de Octubre de 2007, p. 149. Lurá, M.; Vaccari, M.; Latorre Rapela, M.; Mattio, M. & Di Conza, J. Estudio de la variación gené- tica de Cercospora kikuchii mediante la aplicación de marcadores moleculares. 5º Congresso Bra- sileiro de Micología. Recife, Pernambuco, Brasil, 12 a 16 de Novembro de 2007, p. 201. Latorre Rapela, M.G.; Vaccari, M.C.; Di Conza, J.A. y Lurá, M.C. Estudio comparativos de varia- bilidad genética en Cercospora kikuchii mediante el uso de marcadores moleculares.
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